WO2012050198A1 - 多機能分注ユニットを利用した核酸自動処理装置およびその方法 - Google Patents
多機能分注ユニットを利用した核酸自動処理装置およびその方法 Download PDFInfo
- Publication number
- WO2012050198A1 WO2012050198A1 PCT/JP2011/073697 JP2011073697W WO2012050198A1 WO 2012050198 A1 WO2012050198 A1 WO 2012050198A1 JP 2011073697 W JP2011073697 W JP 2011073697W WO 2012050198 A1 WO2012050198 A1 WO 2012050198A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nozzle
- nucleic acid
- sealing
- liquid
- container
- Prior art date
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
- C12M1/38—Temperature-responsive control
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0275—Interchangeable or disposable dispensing tips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/142—Preventing evaporation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/02—Identification, exchange or storage of information
- B01L2300/024—Storing results with means integrated into the container
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0654—Lenses; Optical fibres
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0672—Integrated piercing tool
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1888—Pipettes or dispensers with temperature control
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/143—Magnetism, e.g. magnetic label
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
- G01N2035/00742—Type of codes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
- G01N2035/00821—Identification of carriers, materials or components in automatic analysers nature of coded information
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
- G01N2035/00821—Identification of carriers, materials or components in automatic analysers nature of coded information
- G01N2035/00851—Identification of carriers, materials or components in automatic analysers nature of coded information process control parameters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0403—Sample carriers with closing or sealing means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/103—General features of the devices using disposable tips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1048—General features of the devices using the transfer device for another function
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1048—General features of the devices using the transfer device for another function
- G01N2035/1055—General features of the devices using the transfer device for another function for immobilising reagents, e.g. dried reagents
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1048—General features of the devices using the transfer device for another function
- G01N2035/1062—General features of the devices using the transfer device for another function for testing the liquid while it is in the transfer device
Definitions
- the present invention relates to a nucleic acid automatic processing apparatus using a multi-function dispensing unit and a method therefor.
- nucleic acids DNA, RNA, etc.
- fragments thereof oligonucleotides, nucleotides, etc.
- analysis of gene expression level it is possible to know the relative quantity ratio of each nucleic acid. Must be amplified. Therefore, by using a real-time PCR method and a device equipped with a thermal cycler and a spectrofluorometer, the analysis of electrophoresis is not required by detecting and analyzing the production process of DNA amplification products in PCR in real time. .
- the SPIA Single Primer Isothermal Amplification
- a linear DNA amplification method based on an isothermal reaction using a DNA / RNA chimera primer, DNA polymerase, and RNaseH has come to be used.
- Such temperature control in nucleic acid amplification and the like is performed by using a material such as aluminum for a container formed of polypropylene or the like containing the necessary reagents such as template DNA, primer, DNA polymerase, nucleotide and reaction buffer solution. It is accommodated in the accommodating part of the formed block-shaped thermostat, the metal block-shaped accommodating part is heated or cooled, and waiting until the liquid temperature has an even temperature distribution, the constant temperature or the next temperature Heating or cooling is performed (Patent Documents 1, 2, and 3).
- sealing the container for temperature control with a lid prevents entry of foreign matter from the outside, prevents leakage of liquid from the inside, and the reaction liquid in the container is heated or cooled. It is particularly necessary to eliminate the influence of outside air and outside air as much as possible until a uniform liquid temperature distribution is obtained.
- Patent Document 4 when the temperature is controlled, even if the lid is removed from the container, the lid may be in close contact with the opening of the container due to moisture, making it difficult to open the lid, and there is a possibility that rapid processing cannot be performed. . In addition, when the lid is opened, the liquid adhering to the inside of the lid may drip or scatter, which may cause contamination (Patent Document 4).
- nucleic acid amplification in order to perform nucleic acid amplification, as a premise, a very small amount of nucleic acid is extracted from a specimen, and the nucleic acid is used as a template DNA, together with various reagents, primers, DNA polymerase, nucleotides, reaction buffers, etc. For example, it is necessary to perform processing manually or using various apparatuses, and the present situation is that a specialist researcher or engineer regarding nucleic acid is required.
- a first object of the present invention is to use a multifunctional dispensing unit for processing of nucleic acids including at least nucleic acid extraction to nucleic acid amplification.
- a nucleic acid automatic processing apparatus using a multi-function dispensing unit that can be consistently automated, reduce user labor, and can be manufactured and used quickly and efficiently without expanding the scale of the apparatus, and It is to provide that method.
- the second object of the present invention is to provide an automatic nucleic acid processing apparatus using a multi-function dispensing unit and a method thereof that enables highly reliable optical measurement of a solution in a reaction vessel in which nucleic acid amplification is performed. is there.
- Its third purpose is to enable dispensing, temperature control and / or optical measurement to the reaction vessel in which nucleic acid amplification is performed without manual operation, so that the reaction vessel can be externally supplied.
- a nozzle head having one or two or more nozzles to which a dispensing tip capable of sucking and discharging a liquid by the suction and discharging mechanism is detachably mounted.
- a container group having at least one or two or more liquid storage units and one or two or more reaction containers for storing an amplification solution used for nucleic acid amplification, and relatively movable between the nozzle and the container group
- the amplifying solution and / or a sealing lid that can be transported and contained in the reaction vessel can be sealed in the reaction vessel, and the accommodation of the amplification solution in the reaction vessel is completed.
- a sealing controller that controls the suction / discharge mechanism and the moving mechanism, or the moving mechanism so that the sealing liquid and / or the sealing lid seal the amplification solution in the reaction container. It is an automatic nucleic acid processing device that uses an injection unit.
- a desorption mechanism for desorbing the dispensing tip from the nozzle on a stage provided with a nozzle head or a container group.
- a dispensing tip or the like it is accommodated in the tip accommodating portion provided in the container group in a state where the nozzle mounting opening of the dispensing tip is on the upper side.
- the nozzle can be mounted by inserting the lower end of the nozzle into the opening for mounting the nozzle by moving the nozzle and the tip accommodating portion in a direction of approaching by the vertical movement mechanism of the moving mechanism.
- the nozzle head is provided with a magnetic part capable of exerting and removing magnetic force in the dispenser chip mounted in order to extract a substance such as DNA from a specimen.
- the “desorption mechanism” for example, a plate having a hole having a diameter larger than the outer diameter of the nozzle but smaller than the thickest portion of the dispensing tip is provided in the nozzle head or the stage, There is a mechanism for detaching the mounted dispensing tip by relatively lowering or raising the plate along the axial direction of the nozzle penetrating each hole.
- the attachment / detachment is performed by interlocking the plate with a drive mechanism of a piston that slides in a cylinder as the suction / discharge mechanism, and the plate is provided on the stage. In such a case, the nozzle is moved in the vertical direction relative to the plate by the vertical movement mechanism.
- the container group provided on the stage includes a plurality of liquid storage units for storing liquids such as reaction vessels, specimens, and reagents, for example, amplification solutions used for nucleic acid amplification, the dispensing tips, and openings of the container groups. It is preferable to provide a chip accommodating portion that accommodates a chip such as a perforating chip for perforating a film provided to cover the film.
- the container group includes a microplate in which wells as a plurality of liquid storage portions are arranged in a matrix or a row (row), and a cartridge-like container in which wells as a plurality of liquid storage portions are arranged in a row. Including.
- Amplification solution means, for example, a template DNA solution, primer solution, DNA polymerase solution, nucleotide solution, reaction buffer solution or the like to be amplified when amplification is performed by PCR, and amplification is performed by SPIA These include DNA / RNA chimera primer solution, DNA polymerase solution, RNase H solution and the like.
- SPIA amplification is performed by SPIA
- DNA / RNA chimera primer solution DNA polymerase solution
- RNase H solution RNase H solution
- the “intercalation method” uses the property that fluorescent substances such as SYBR (registered trademark) GREEN I and ethidium bromide enter double-stranded DNA during the extension reaction and emit fluorescence when irradiated with excitation light. It is a method of measuring quantity. Therefore, the amplification solution contains at least the fluorescent substance and a quencher that suppresses light emission of the fluorescent substance.
- the “hybridization method” is a method of detecting only a target PCR product using a DNA probe labeled with a fluorescent substance in addition to a PCR primer. That is, the hybridized DNA (amount) is detected by hybridization of the fluorescently labeled DNA probe with the target PCR product.
- the “LUX method” utilizes the property that the fluorescent signal of a fluorescent substance labeled on an oligonucleic acid is influenced by the shape (sequence, single strand, double strand, etc.) of the oligonucleic acid.
- real-time PCR is performed using a PCR primer (LUX primer) labeled with one type of fluorescent substance and a PCR primer not labeled with anything.
- the LUX primer has a fluorescent substance labeled near the 3 ′ end and is designed to take a hairpin structure with the 5 ′ end. When the LUX primer has a hairpin structure, the quenching effect is released and the fluorescence signal increases. By measuring this signal increase, the amount of PCR product can be measured.
- the nozzle or the dispensing tip attached to the nozzle reaches these containers by the moving mechanism, and can suck and discharge liquid, or attach and detach the tip.
- Examples of materials such as a container including the reaction container and a lid include resins such as polyethylene, polypropylene, polystyrene, and acrylic, glass, metal, and metal compounds.
- the size of the container is, for example, a size that can accommodate a liquid of several ⁇ l to several hundreds of ⁇ l and can be inserted into the tip of the dispensing tip.
- the diameter of the size of one container is several millimeters to several tens of millimeters, and the depth is several millimeters to several tens of millimeters.
- the “dispensing tip” includes, for example, a large-diameter portion, a small-diameter portion, and a transition portion that communicates the large-diameter portion and the small-diameter portion. It has a mounting opening to be inserted and mounted on the nozzle, and the narrow-diameter portion has a front end opening through which liquid can flow in and out by suction and discharge of gas by the suction and discharge mechanism.
- the dispensing tip and the nozzle are made of, for example, organic materials such as resins such as polypropylene, polystyrene, polyester, and acrylic, metals such as glass, ceramics, and stainless steel, metal compounds, and inorganic materials such as semiconductors.
- the “temperature controller” has a temperature source capable of raising or lowering the temperature in the reaction container containing the liquid to be temperature controlled based on an external signal or the like.
- the block-like member is provided with, for example, a Peltier element, a heater, a cooling device, and the like. In order to perform a process such as PCR, a thermal cycler using a Peltier element is preferable as the temperature controller.
- Temperature control means that a target liquid or container is maintained at one or two or more set predetermined temperatures for a set time in accordance with a set order. It is. The temperature controller is instructed by sending a corresponding signal based on a program.
- the “predetermined temperature” is a target temperature to be reached by an object such as a target liquid.
- a nucleic acid such as DNA contained in the liquid or an oligonucleotide that is a fragment of the nucleic acid is amplified by a PCR method.
- the predetermined temperature to be set is, for example, a temperature cycle performed by the PCR method, that is, each temperature necessary for DNA denaturation, annealing or hybridization, extension, about 94 ° C., 50 ° C. to 60 ° C. Temperatures between 0 ° C., for example about 50 ° C. and about 72 ° C.
- the SPIA method it is set to a constant temperature, for example, 55 ° C.
- the predetermined temperature can be cooled by a temperature controller at a transition promoting temperature lower than these predetermined temperatures.
- a temperature controller at the time of transition from a predetermined temperature of low temperature to a predetermined temperature of high temperature, heating is performed at a temperature for promoting transition higher than these predetermined temperatures, thereby shortening the transition time and reducing one cycle time.
- transition-promoting temperature is the time required to maintain each temperature, and depends on the type of amplification method, the reagent and liquid amount used in the PCR method, the shape, material, size, thickness, etc.
- the total is, for example, several seconds to several tens of seconds
- the processing time of the entire PCR method is, for example, about several minutes to several tens of minutes.
- the transition time is also included in the predetermined time.
- the “suction / discharge mechanism” includes, for example, a cylinder, a piston sliding in the cylinder, a nut portion connected to the piston, a ball screw into which the nut portion is screwed, and the ball screw in both forward and reverse directions. It has a motor that rotates and a pump mechanism.
- the “movement mechanism” for example, there is a mechanism that enables movement between the reaction vessel, that is, a stage in which the reaction vessel is provided, and a nozzle relatively in an axial direction and a horizontal plane of the nozzle.
- the movement in the horizontal plane for example, the stage or nozzle moves along the X axis and the Y axis, the XY axis movement mechanism that moves, or the Y (X) axis movement mechanism that moves only along the Y axis or the X axis.
- As the movement of the nozzle in the axial direction there is a vertical movement mechanism provided in the nozzle head that moves the nozzle in the axial direction (Z-axis direction). An actuator to be described later is driven by this moving mechanism so as to be interlocked with the nozzle.
- “Sealing liquid and / or sealing lid” means either a sealing liquid, a sealing lid, or a sealing liquid and a sealing lid, and the temperature of the sealing lid is controlled, for example,
- the reaction vessel is sealed by closing the opening of the reaction vessel by fitting or the like, and the sealing liquid does not react with the amplification solution contained in the reaction vessel, and these solutions
- the “predetermined accommodating portion” is a liquid accommodating portion if it is a sealed liquid, and a sealed lid accommodating portion if it is a sealed lid.
- the sealing control unit needs to control the suction / discharge mechanism and the moving mechanism, and to seal with the “sealing lid”. It is necessary to control the moving mechanism.
- the sealing lid In order to transport the sealing lid to the reaction vessel, the sealing lid needs to be detachably attached to the tip of the nozzle by fitting, screwing, friction, adsorption or the like.
- the dispensing tip In order to transport the amplification solution, the dispensing tip is used after the dispensing tip is attached to the nozzle. Therefore, in order to attach the sealing lid with the same nozzle, the dispensing tip is detached. It becomes necessary to do.
- sealing liquid When sealing liquid is used, during temperature control, an oil film is formed to prevent evaporation of the amplification solution, and due to the heat insulation effect, condensation on the sealing lid is prevented, so that the sealing lid can be opened and closed easily. To. Further, it is possible to prevent the gas from being contained in the amplification solution and perform uniform temperature control.
- the use of a sealing liquid eliminates the need for a sealing lid at the time of temperature control, and air does not enter between the oil film and the amplification solution, so that the liquid and the oil film are sealed during temperature control. The mechanism to make it unnecessary becomes unnecessary.
- the structure can be simplified without the need to heat or shake the sealing lid. Even when the temperature is controlled by sealing with a sealing liquid, it is preferable to seal with a sealing lid after the temperature control in terms of management of the amplification solution.
- the “sealing control unit” includes a computer (CPU) built in the nucleic acid automatic processing apparatus and a program for driving the computer. For example, the control unit of each mechanism for driving the nozzle and the moving mechanism through a DA converter The airtight control is performed by sending to.
- the container group includes a specimen, a magnetic particle suspension in which magnetic particles capable of capturing a nucleic acid to be amplified or a fragment thereof are suspended, and separation and extraction used for separation and extraction of the amplification target. It further has two or more liquid storage units for storing a solution, and can apply and remove a magnetic field to the inside of the dispensing tips attached to the nozzles or the liquid storage units provided in the container group.
- the solution further has a magnetic force part capable of adsorbing the magnetic particles on the inner surface of the injection chip or the liquid storage part, and controls the suction / discharge mechanism, the moving mechanism, and the magnetic force part, and the solution to be amplified from the specimen Is a nucleic acid automatic processing apparatus using a multi-function dispensing unit further having an extraction control unit that separates and extracts the sample and stores it as a part of the amplification solution in the liquid storage unit.
- the “solution for separation and extraction” refers to a lysis solution that decomposes or dissolves a protein that forms a cell wall or the like contained in a specimen and causes nucleic acid or a fragment thereof to flow out of bacteria or cells, nucleic acid to the magnetic particles, or There are a buffer solution for facilitating the capture of the fragment, and a dissociation solution for dissociating the nucleic acid or the nucleic acid fragment captured by the magnetic particle from the magnetic particle. In order to separate the nucleic acid or a fragment thereof, it is preferable to repeat the suction and discharge of the mixed solution.
- a suction / discharge mechanism for sucking and discharging a gas
- a nozzle provided with one or more nozzles for detachably mounting a dispensing tip capable of sucking and discharging a liquid by the suction / discharge mechanism
- Suspended magnetic particles capable of capturing the head and one or more liquid storage units for storing the amplification solution used for nucleic acid amplification, one or more reaction vessels, the specimen, the nucleic acid to be amplified or a fragment thereof.
- Magnetic particle suspension two or more liquid storage units for storing the separation / extraction solution used for separation and extraction of the amplification target, and two or more tip storage units for storing one or two or more dispensing tips in a mountable manner
- a container group having at least, a detachable mechanism that can detach the dispensing tip from the nozzle, a moving mechanism that can relatively move between the nozzle and the container group, and the reaction volume
- a temperature controller capable of controlling the temperature for nucleic acid amplification, and a container that is housed in a predetermined container other than the reaction container of the container group and can be transported to the reaction container using the nozzle, and the reaction
- the solution for amplification contained in a container has a sealing liquid and / or a sealing lid that can be sealed in the reaction container, and a liquid storage part provided in the dispensing tip or the container group attached to the nozzle.
- a magnetic part capable of exerting and removing a magnetic field therein and capable of adsorbing the magnetic particles on an inner wall of the dispensing tip or the liquid container; the suction / discharge mechanism; the moving mechanism; the desorption mechanism;
- the dispensing tip is attached to the nozzle by controlling the magnetic force portion, and the solution to be amplified is separated and extracted from the sample and accommodated as a part of the amplification solution in the liquid accommodating portion.
- the extraction control unit desorbed from the nozzle and the storage of the amplification solution in the reaction container are completed, the sealing liquid and / or the sealing lid seal the amplification solution in the reaction container.
- An automatic nucleic acid processing apparatus using a multi-function dispensing unit having a suction / discharge mechanism and the moving mechanism, or a sealing controller for controlling the moving mechanism.
- a fourth aspect of the invention is a measuring unit capable of measuring an optical state including light emission, coloration, color change or light change generated in the amplification solution sealed in the reaction vessel by the sealing liquid and / or a sealing lid.
- the nozzle head is provided with one or more measurement ends that receive light based on the light emission, etc., and after the amplification solution containing the amplification target is sealed in the reaction vessel or when the sealing is performed.
- An automatic nucleic acid processing apparatus using a multi-function dispensing unit having a measurement control unit that enables the measurement by controlling the moving mechanism so that a measurement end approaches the reaction vessel.
- the measurement unit is provided with an excitation light irradiation unit that emits excitation light and a fluorescence light receiving function.
- the place where the measurement end is provided is, for example, a light receiving end or a light receiving end that is an end of an optical fiber included in the measurement unit as the measurement end, and an irradiation end on the tip surface of the nozzle.
- multiple types of amplification can be performed in parallel in the same conditions in a single reaction vessel. It is possible to perform multiplex PCR amplification and multiplex real-time PCR on a target by using primers labeled with a plurality of types of luminescent substances.
- the measurement end is provided in the nozzle head, the sealing liquid and / or the sealing lid have translucency, and the measurement control unit supplies the amplification solution in the reaction container. It is an automatic nucleic acid processing apparatus using a multi-function dispensing unit that controls the moving mechanism so that the optical state can be measured from the upper side through the sealed liquid and / or sealed lid.
- the measurement unit is connected to the suction / discharge mechanism and is capable of receiving or irradiating light through a tip surface of the nozzle provided with an opening of a flow tube passing through the nozzle.
- the nucleic acid automatic processing apparatus uses a multifunctional dispensing unit in which a fiber is provided so as to pass through the nozzle, and the tip surface corresponds to the measurement end.
- the flow tube is connected to the suction / discharge mechanism via a pipe line, and the optical fiber outside the nozzle is provided along a path direction different from the pipe line,
- the light receiving unit and the irradiation unit to which the optical fiber is connected can be formed compactly on the nozzle head.
- the measurement end of the measurement unit is provided in the nozzle head so as to be linked with the nozzle at a predetermined interval from the tip of the nozzle, and the opening of the reaction vessel of the container group.
- the “predetermined interval” is determined, for example, on the stage of the multi-function dispensing unit so that the nozzle and the measurement end do not simultaneously come into contact with the accommodating portion in the container group. Note that if the predetermined interval is made large so that the measurement end can be made to have a size capable of entering and irradiating the light beam with respect to the entire liquid surface of the portion containing the amplification solution, reliability can be improved from the amplification solution. High processing can be performed. For that purpose, it is necessary to have an optical opening having a size enough to cover the surface of the reaction container in which the amplification solution is accommodated.
- the predetermined interval When a plurality of sets of nozzles and measurement units are used, it is preferable to set the predetermined interval so that a measurement end corresponding to a certain nozzle does not enter a later-described dedicated area corresponding to another nozzle.
- the measurement end preferably has an optical opening that is determined according to the width and shape of the opening of the portion of the container group that accommodates the amplification solution of the reaction container. In the above measurement part, it is possible to cope with various fluorescent materials and luminescent materials by making it possible to select a plurality of types of wavelengths or wavelength bands of fluorescence or excitation light.
- the sealing control unit attaches the dispensing tip to the nozzle, and then sucks a predetermined amount of the sealing liquid from the predetermined accommodating portion of the container group with the dispensing tip.
- Multi-function for controlling the suction / discharge mechanism and the moving mechanism so as to transport the sealing liquid by discharging the sealing liquid into the reaction container containing the solution and seal the amplification solution in the reaction container.
- the temperature-controllable reaction vessel comprises a narrow tube portion or a thin tube portion in which the amplification solution is accommodated, and the thin tube portion or the thin tube portion communicates with the thin tube portion or the thin tube portion.
- a wide-mouthed tube part that has an opening wider than the opening of the thin tube part or the thin tube part and accommodates the sealing liquid, and the sealing control unit is based on the amount of the amplification solution
- An automatic nucleic acid processing apparatus using a multi-function dispensing unit that controls the amount of the sealing liquid reaching the wide-mouthed tube portion to be accommodated in the reaction vessel.
- the reason for the configuration of the reaction vessel is that, when measuring the reaction vessel sealed with the sealing liquid from above, if the spread of the sealing liquid is small, the surface tension between the inner wall of the reaction vessel This is because the surface forms a curved surface with a large curvature and light scattering occurs, which may make measurement difficult.
- the amplification solution needs to be stored in a compact container as much as possible in order to enable uniform temperature control. This is because, as described above, the shape that accommodates the sealing liquid and the portion that accommodates the amplification solution are used. *
- the sealing lid has a fitting portion that can be fitted and fitted to the tip of the nozzle, and has a desorption mechanism for detaching the sealing lid and the dispensing tip from the nozzle.
- the sealing controller attaches a dispensing tip to the nozzle and accommodates the amplification solution in the reaction container, and then removes the dispensing tip from the nozzle and attaches the sealing lid to the nozzle so that the amplification solution is added to the nozzle.
- An automatic nucleic acid processing apparatus using a multi-function dispensing unit that controls the suction / discharge mechanism, the moving mechanism, and the desorption mechanism so as to be sealed in a reaction container.
- the sealing lid is accommodated with the fitting portion facing upward so that the sealing lid can be mounted by lowering the tip of the nozzle.
- the container group includes the sealing liquid and a sealing lid
- the sealing lid includes a fitting portion that can be fitted and attached to a tip portion of the nozzle.
- the sealing control unit when the storage of the amplification solution in the reaction container is completed, after transporting the sealing liquid to the predetermined storage unit, A multi-function dispensing unit that controls the moving mechanism and the desorption mechanism so that the dispensing tip is detached from the nozzle, the sealing lid is attached to the nozzle, transported to the reaction container, and the opening is sealed; This is an automatic nucleic acid processing apparatus used.
- the sealing liquid covers the amplification solution, air can be prevented from scattering due to temperature control for heat insulation, and not only can the scattering of the amplification solution be prevented, but also translucency. Since it is possible to prevent the condensation on the sealing lid having a certain degree of reliability, highly reliable measurement from the outside can be performed.
- the relationship between the sealing liquid accommodated in the reaction container and the sealing lid attached to the reaction container is not only when they are separated from each other, but also when the sealing liquid and the sealing lid are in contact with each other, or This includes the case where a part of the sealing lid is immersed in the sealing liquid.
- the measurement can be performed with the air layer removed from the outside of the reaction vessel by performing the measurement through the immersed part.
- the sealing control unit controls the suction / discharge mechanism or the moving mechanism to press or shake the sealing lid.
- This is an automatic nucleic acid processing apparatus using a function dispensing unit.
- the pressing or shaking is performed by, for example, a moving mechanism that moves the nozzle along the Z axis.
- the suction / discharge mechanism includes a cylinder communicating with the nozzle, a piston that slides in the cylinder, and a piston drive mechanism that drives the piston
- the nozzle head is provided in conjunction with the piston.
- the operating tool has, for example, a rod shape, a cylindrical shape, a cone shape, or the like provided separately from the sealing lid, and the lower end portion of the member can contact the sealing lid.
- a heating unit for heating the sealing lid is provided at the tip of the nozzle, and the sealing control unit is configured so that the sealing lid is not contained in the reaction vessel.
- the nucleic acid automatic processing apparatus using a multi-function dispensing unit that controls the heating unit so as to heat the sealing lid after sealing the opening of the reaction vessel.
- the heating of the sealing lid by the heating unit is performed to prevent condensation during temperature control of the reaction vessel sealed by the sealing lid.
- the container group accommodates or accommodates a liquid accommodating portion group composed of a plurality of containers formed in a single line capable of accommodating or accommodating a liquid, and an instrument used by being attached to the nozzle. It consists of one or two or more storage units connected in parallel to a device storage unit group consisting of a plurality of storage units formed in a single row, and the liquid storage unit group includes at least the temperature controller.
- One or more temperature-controllable reaction vessels, separation / extraction solutions used for separation and extraction of specimens, nucleic acids or fragments thereof, magnetic particle suspensions and amplification solutions used for amplification of nucleic acids or fragments thereof are stored in advance.
- a reagent storage unit group that can be stored, and the instrument storage unit includes at least one or more dispensing tips that can be attached to the nozzle and a punching chip for punching a prepack film.
- the combination of the liquid storage unit group formed in a single row and the instrument storage unit group formed in a single row completes one process for one specimen or another liquid storage unit group in one row and 1 It has the number of liquid storage portions and the device storage portions used to complete the process for one specimen in combination with the device storage portion group formed in a row.
- the “instrument” is an instrument to be used by being attached to or supported by the nozzle of the multi-function dispensing unit.
- a predetermined biological substance is fixed inside or detachable from the outside.
- accommodating the instrument includes a case where the wall covering the instrument to be accommodated is not necessarily required and the instrument is fitted and held in the hole.
- the liquid storage unit group of the container group has an identification information display unit that displays identification information for identifying the liquid storage unit group, and the device storage unit group identifies the device storage unit group.
- the multifunctional dispensing unit has an identification information reading unit for reading the identification information displayed on the identification information display unit, and based on these identification information. It is preferable to instruct the controller to perform nucleic acid processing.
- the “identification information” includes, for example, “sample information” and “examination information”.
- the sample information is information necessary for identifying or managing the sample.
- the sample is collected.
- Patients are attributed to specimens such as animals, food, soil, sewage, for example, patient's name, age, gender, ID number, food sales location, soil collection location, collection date and time, or physical properties of the collected specimen, For example, the type of patient's blood, urine, feces, body fluid, cell, etc., the type of food, the type of soil, the type of sewage, etc.
- the information for managing the sample includes, for example, the sampler of the sample, the collection date, the person in charge of the test for the sample, the date of the test for the sample, and the like.
- “Examination information” is information indicating the contents of an examination performed on a specimen, and includes, for example, examination items such as infectious diseases (identification of influenza, foot-and-mouth disease of livestock, etc.), autoimmune diseases (collagen, etc.) Disease, DNA antibody), various gene information (for example, SNPs, base sequence determination), type of reagent used in genetic diagnosis or testing, reagent production lot number, calibration curve of reagent, type of testing instrument, structure, etc. Can be contained.
- the identification information is displayed by handwriting, when printed, by barcode, or by a QR (registered trademark) code (dimensional code in the matrix direction).
- the “identification information reading unit” is, for example, a digital camera, and captured image data can be easily taken into the memory of a computer using, for example, a USB code. This eliminates the need for an operator to input the information using a computer keyboard. Further, the data can be easily transmitted, processed or duplicated and applied in various cases. For example, the image data may be transmitted by providing a communication unit in the sample testing apparatus.
- a suction / discharge mechanism that sucks and discharges gas
- a nozzle head having two or more nozzles to which a dispensing tip is detachably mounted, one nozzle enters, and no other nozzle enters
- One or two or more liquid storage units that are arranged in two or more dedicated regions corresponding to each nozzle and store amplification solutions used for nucleic acid amplification, and magnetic particles in which magnetic particles capable of capturing nucleic acids or fragments thereof are suspended
- a container group having at least a sealing liquid and / or a sealing lid that can be transported to the reaction container and can seal the amplification solution contained in the reaction container in the reaction container;
- a moving mechanism that allows relative movement between a nozzle and each container group and restrict
- the magnetic particle suspension and the distribution by the dispensing tip are controlled. Separation and extraction of a nucleic acid or a fragment thereof from a specimen using an extraction solution, mixing of the amplification solution containing the nucleic acid or a fragment thereof extracted by the dispensing chip, the amplification by the sealing liquid and / or a sealing lid
- a multi-function dispensing unit having a nucleic acid processing control unit for sealing the solution to the reaction container, controlling the temperature, and instructing the measurement of the optical state by bringing the measurement end close to the sealed reaction container This is an automatic nucleic acid processing apparatus used.
- the nucleic acid processing control unit includes the extraction control unit, the sealing control unit, and the measurement control unit, and further controls between these control units to perform nucleic acid extraction, nucleic acid amplification, and nucleic acid measurement. Can be done consistently using a multi-function dispensing unit.
- These sealing control unit, extraction control unit, measurement control unit, and nucleic acid processing control unit are constituted by a CPU built in the nucleic acid automatic processing apparatus and a program for driving the CPU.
- the liquid can be sucked and discharged by a suction / discharge mechanism that sucks and discharges gas from one or two or more liquid storage portions that store the amplification solution used for nucleic acid amplification of the container group.
- a suction / discharge mechanism for sucking and discharging the gas
- a moving mechanism for relatively moving between the nozzle and the container group The amplification solution is transported to the reaction vessel controlled for temperature amplification for nucleic acid amplification, and the suction / discharge mechanism and the moving mechanism, or the moving mechanism from a predetermined container other than the reaction container in the container group.
- a multi-machine that transports a sealing liquid and / or a sealing lid into the reaction container using the nozzle to seal the amplification solution in the reaction container, and controls the temperature in the reaction container.
- a dispensing unit automated nucleic acid processing method using a.
- the sealing of the reaction container with the sealing lid may be performed in order to seal the sealing solution and the amplification solution in the reaction container so as not to leak during temperature control and after the temperature control is completed.
- the container group includes a specimen, a magnetic particle suspension in which magnetic particles capable of capturing a nucleic acid to be amplified or a fragment thereof are suspended, and separation and extraction used for separation and extraction of the amplification target.
- It further has two or more liquid storage units for storing a solution, and a chip storage unit for storing one or two or more dispensing tips, and the sample and the sample as the solution for separation and extraction using the dispensing tip Mixing and reacting with a solution for decomposing or dissolving the protein contained in the mixture, reacting the reaction solution with the magnetic particle suspension, causing the magnetic particles to capture the amplification target, and the nozzle
- the magnetic particles are separated by adsorbing the magnetic particles to the inner wall of the dispensing tip or the liquid storage unit by applying a magnetic field to the dispensing tip or the liquid storage unit using a magnetic part provided in the head, and the container Housed in a flock
- the dissociation liquid as another separation and extraction solution is brought into contact with the magnetic particles, the amplification target is dissociated from the magnetic particles, and the amplification target solution is placed in the liquid container as a part of the amplification solution.
- This is an automatic nucleic acid processing method using a multifunctional dispensing unit to
- the measuring end is brought close to the reaction vessel using the moving mechanism, and light in the amplification solution is emitted.
- a multi-functional dispensing unit that receives light and measures the optical state including light emission, coloration, color change, and light change generated in the amplification solution sealed in the reaction vessel and / or sealed with a sealing lid This is an automatic nucleic acid processing method.
- the measurement end provided in the nozzle head is used to seal the amplification solution in the reaction vessel and / or a sealing lid using the moving mechanism.
- the nucleic acid automatic processing method using a multifunctional dispensing unit that is positioned on the upper side of the substrate and measures the inside of the amplification solution through a light-transmitting sealing liquid and / or a sealing lid.
- the measurement step is performed by moving the measurement end provided in the nozzle head so as to be spaced apart from the tip of the nozzle at a predetermined interval and interlocking with the nozzle.
- This is an automatic nucleic acid processing method using a multi-function dispensing unit that enters or irradiates a light bundle corresponding to the size and shape of the opening of the portion containing the solution via the sealing liquid and / or the sealing lid.
- the sealing liquid is supplied to the predetermined group of the container group using the dispensing tip, the suction / discharge mechanism, and the moving mechanism.
- It is an automatic nucleic acid processing method using a multi-function dispensing unit that seals the amplification solution in the reaction container by transporting a predetermined amount from a container and discharging it into the reaction container.
- the “predetermined amount” is an amount that can cover the entire surface of the amplification solution, and is determined by the shape of the reaction vessel and the volume of the stored amplification solution.
- the sealing is performed by attaching a dispensing tip to a nozzle and storing the amplification solution in a reaction container, and then detaching the dispensing tip from the nozzle. It is a nucleic acid automatic processing method using a multi-function dispensing unit that is mounted on the nozzle at the fitting portion and transported to the reaction vessel and closes the opening of the reaction vessel.
- the sealing liquid is transported to the reaction container by a predetermined amount, and the dispensing tip is detached from the nozzle.
- a nucleic acid automatic processing method using a multi-function dispensing unit having a step of attaching the sealing lid to the nozzle, transporting it to the reaction container and fitting it into the opening.
- the “predetermined amount” is, for example, an amount that can cover the entire surface of the amplification solution and can close the reaction vessel with the sealing lid. This amount is determined based on the shape of the reaction vessel, the volume of the stored amplification solution, and the shape of the sealing lid.
- the sealing lid is attached to the nozzle and transported to the reaction container to close the opening, and the sealing lid is attached to the nozzle and pressed by the nozzle or the suction / discharge mechanism.
- the sealing lid is attached to the nozzle and pressed by the nozzle or the suction / discharge mechanism.
- it is the nucleic acid automatic processing method using the multifunctional dispensing unit which has the process to shake.
- the opening of the reaction container is closed with the sealing lid, and then the nozzle is used for temperature control of the reaction container.
- This is a nucleic acid extraction amplification processing method or the like using a multi-function dispensing unit that heats the sealing lid by heating the tip of the tube.
- the heating of the sealing lid is for preventing condensation generated on the sealing lid.
- the specimen and magnetic particle suspension provided in each of the dedicated groups in which one of the nozzles enters and the other nozzles do not enter with respect to two or more nozzles provided in the nozzle head A dispensing tip that is detachably attached to the nozzle, a suction / discharge mechanism that performs gas suction / discharge, and the nozzle;
- the sample and the separation / extraction solution are transported to the reaction vessel and mixed by using a moving mechanism that allows movement relative to the container group and restricts movement of each nozzle within the dedicated region.
- the magnetic particle suspension is mixed and reacted to cause the magnetic particles to capture nucleic acids or fragments thereof obtained from the specimen, and the magnetic particles provided in the nozzle head are used for the separation.
- the magnetic particles are adsorbed and separated on the inner wall by applying a magnetic field to the chip or the liquid container, and the nucleic acids or fragments thereof are dissociated by bringing the separated magnetic particles into contact with the dissociation liquid, thereby dissociating the nucleic acids.
- a fragment thereof is housed and mixed as a part of an amplification solution in a reaction container whose temperature can be controlled by the suction / discharge mechanism and the transfer mechanism, and the amplification solution is mixed with the suction / discharge mechanism and the transfer mechanism, or
- the reaction solution is sealed in the reaction container with a sealing liquid and / or a sealing lid accommodated in the container group by a moving mechanism, the temperature of the sealed amplification solution is controlled using the temperature controller, and the sealing liquid and / or An optical state including light emission, coloration, color change or light change generated in the amplification solution sealed in the reaction container by a sealing lid is placed at the measurement end of the measurement unit at the measurement end.
- the sealing liquid and / or the sealing lid is provided with a suction / discharge mechanism.
- the amplification solution is controlled to be sealed in the reaction vessel using an original dispensing function having a moving mechanism and a transfer mechanism, thereby providing the device with a sealing function for the amplification solution, thereby providing a large number of dispensing devices.
- nucleic acid amplification processing that requires high precision can be performed without touching the solution to be introduced into the reaction vessel, the container that contains the solution, the reaction vessel itself, and the accessories of the reaction vessel. Made it possible to perform processing. Accordingly, it is possible to perform highly reliable automatic nucleic acid processing that can reduce the manufacturing cost and reliably prevent cross contamination without increasing the scale of the apparatus.
- the separation and extraction of the nucleic acid to be amplified or the fragment thereof from the sample is also performed by using the dispensing function inherent in the multifunction dispensing unit. , Increase the work efficiency of nucleic acid amplification processing of the device, and can continuously perform a series of processing from separation and extraction of nucleic acid from specimen to amplification using the same device function continuously and without interruption . Therefore, the scale of the apparatus can be prevented from increasing, the flow of the process can be continuous and smooth, a series of processes can be performed quickly and efficiently, and an inexpensive apparatus can be provided.
- the dispensing tip is provided and the dispensing tip is accommodated in a state that can be attached to the nozzle, thereby automatically attaching the dispensing tip by using the original dispensing mechanism.
- the dispensing tip can be removed from the prepared nozzle and sealed with a sealing lid, and the dispensing tip (separation tip) has a relatively large capacity to be used for separation and extraction of the amplification target from the specimen. )
- the dispensing tip to prevent cross-contamination by attaching a dispensing tip with a relatively small volume suitable for amplification processing of the extracted amplification target, and from separation and extraction of the amplification target from the sample to amplification This can be performed more smoothly.
- the treatment based on nucleic acid amplification is originally performed for the measurement of the amplification solution sealed with the sealing liquid and / or the sealing lid. It was made possible by controlling the moving mechanism using the moving mechanism used for the dispensing function, and it was possible to measure quantitative PCR such as real-time PCR.
- the measurement function for measuring the optical state in the amplification solution that has been sealed by the sealing function is provided by using the moving mechanism of the dispensing device, and is further multifunctional.
- the sealing liquid and / or the sealing lid since the sealing liquid and / or the sealing lid has translucency, dispensing that the measurement end is located above the opening of the reaction vessel is performed. Since the optical state in the amplification solution can be reliably measured via the sealing liquid and / or the sealing lid in the same positional relationship as when performing, it is easy to control and can be combined smoothly with dispensing processing, etc. Can do.
- the optical fiber passing through the nozzle is used as the measurement unit and the tip end surface of the nozzle is used as the measurement end, it continues even after the opening of the reaction vessel is closed with the sealing liquid and / or the sealing lid. Since the measurement can be performed at the nozzle position or again at the nozzle position, the working efficiency is high. In addition, the measurement end can be measured in the same positional relationship as when dispensing is performed by positioning the nozzle above the opening of the reaction vessel, so control is much easier and it is smoothly combined with dispensing processing etc. The processing flow is natural.
- the measurement end since the measurement end is provided in the nozzle head at a predetermined interval from the nozzle and away from the nozzle, it moves in a positional relationship substantially similar to that of the nozzle when dispensing. Since the movement control using the mechanism can be performed, the control is easy and the measurement end can be provided without depending on the nozzle structure as compared with the case where the measurement end is provided in the nozzle. Based on the size and shape of the opening of the reaction container in which the amplification solution is accommodated, a signal with a sufficient amount of light can be obtained from the amplification solution, and reliable and accurate measurement can be performed.
- the amplification solution contained in the reaction vessel is sealed using a sealing liquid. Therefore, since the sealing can be performed with the same control as the normal liquid dispensing process using the suction / discharge mechanism and the moving mechanism, the dispensing process and the like can be smoothly combined and the control is easy.
- a sealed liquid when a sealed liquid is used, a liquid layer such as a liquid film is formed to prevent the reaction solution from evaporating, and a measurement end provided on the solid lid or the front end surface of the nozzle to be fitted by a heat insulating effect. Condensation is prevented, the lid of the solid is easily opened and closed, and the measurement is made clear.
- the sealing liquid eliminates the need for a solid lid during temperature control, and air does not enter between the oil film or liquid layer of the sealing liquid and the amplification solution.
- a mechanism for sealing between the amplification solution and the liquid layer (oil film) of the sealing liquid such as a pressing mechanism, becomes unnecessary.
- the structure can be simplified without the need to shake the oil film.
- the sealing liquid when used, it is generally more translucent than when the sealing lid is used, and a clear measurement can be performed. Even in the case of sealing with only the sealing liquid, it is preferable to seal with a sealing lid after temperature control.
- the amplification solution having a large specific gravity has a small cross-sectional area at the lower thin tube portion or thin tube portion
- the sealed liquid having a low specific gravity has a large liquid surface area or cross-sectional area at the upper wide-mouth tube portion.
- the measurement end preferably has an opening corresponding to the cross-sectional area above the thin tube portion or thin tube portion.
- the dispensing tip is attached to and detached, attached to the nozzle using the moving mechanism of the sealing lid, transported to the reaction container, and the reaction of the amplification solution.
- the movement mechanism and the suction / discharge mechanism are controlled by the same control as the dispensing process. Since it can be realized, smooth, continuous and consistent nucleic acid automatic processing can be realized for dispensing, sealing, and temperature control without increasing the scale of the apparatus.
- the reaction solution is sealed with the sealing lid even after the amplification process is completed, cross contamination due to leakage of the amplification solution can be reliably prevented.
- the eleventh invention or the twenty-third invention by sealing the amplification solution by combining two kinds of sealing liquid and sealing lid, the air layer between the sealing liquid and the sealing lid is eliminated, or The expansion of the air layer is prevented, and the condensation of the sealing lid is prevented by the heat insulating effect of the sealing liquid, so that reliable measurement can be performed. Therefore, it is possible to eliminate the necessity of pressing and heating the sealing lid and simplify the structure. In addition, since the reaction solution is sealed in the reaction container with the sealing lid even after the amplification process is completed, cross contamination can be reliably prevented.
- the opening of the reaction vessel can be reliably closed by pressing the sealing lid. Further, by shaking the sealing lid, the sealed state between the opening and the sealing lid can be quickly and easily released and opened. Therefore, high processing efficiency and reliability can be obtained.
- the sealing lid by heating the sealing lid, condensation can be prevented at the opening of the reaction vessel of the sealing lid even if the sealing liquid is not accommodated in the reaction vessel. Moreover, in this invention, since it carries out by heating the front-end
- At least a liquid storage unit group consisting of a plurality of storage units formed in a single row that can store or store a liquid
- at least a device that can be mounted on a nozzle and used Since there is a storage unit array in which an instrument storage unit group composed of a plurality of storage units formed in a single row is arranged in parallel, the processing of one sample is performed using the liquid storage unit group and the instrument storage unit group.
- the movement distance corresponding to the sum of the lengths of the rows in the longitudinal direction is shortened as a whole to the longer one of the liquid storage unit group or the instrument storage unit group, and only movement at regular intervals is added in the width direction. .
- the apparatus scale can be prevented from being enlarged, and the processing can be performed by moving along a straight line along the row except for the movement when the instrument is attached to and detached from the nozzle. Highly efficient processing can be performed. Further, by separating the liquid storage unit group for storing the liquid from the instrument storage unit group for storing the instrument such as a dispensing tip, the respective structures are simplified, and the storage of the liquid storage unit group and the instrument storage unit group is simplified. Facilitates storage and supply to the case.
- a dedicated area provided for each nozzle in which the nozzles do not enter each other is set, and a nucleic acid or Extraction, amplification, and measurement of the fragments can be performed simultaneously, consistently, and in parallel on multiple types of specimens using the same device, resulting in high processing efficiency, quickness, and cross-contamination. Highly reliable processing can be performed.
- the scale of the apparatus can be reduced and it can be provided at a low cost.
- FIG. 2 is a perspective view showing a first embodiment of an automatic nucleic acid processing apparatus using the multifunction dispensing unit shown in FIG. 1. It is the side view and perspective view which expand and show the nozzle head shown in FIG. It is a perspective view which expands and shows the nozzle head and moving mechanism of the multifunctional dispensing unit shown in FIG.
- FIG. 5 is a perspective view showing a nozzle of the apparatus shown in FIGS. 2 to 4, a partially enlarged view, and a perspective view partially cut away. It is sectional drawing which shows the state at the time of real-time PCR which attached the sealing lid to the nozzle shown in FIG.
- FIG. 10 is a diagram in which a dispensing tip is attached to the apparatus according to the second embodiment shown in FIG. 9. It is sectional drawing which shows the state which attached the sealing lid to the nozzle shown in FIG. 9, and sealed the reaction container. It is a top view which shows the container group example which concerns on 3rd Embodiment of the apparatus shown in FIG.
- FIG. 14 is a partially transparent perspective view of a measurement unit according to a sixth exemplary embodiment of the measurement unit illustrated in FIG. 13. It is a perspective view which concerns on the 7th Embodiment about the nucleic acid automatic processing apparatus using the multifunctional dispensing unit shown in FIG. It is a top view which expands and shows the container group provided on the stage of the multifunctional dispensing unit shown in FIG.
- FIG. 1 shows an automatic nucleic acid processing apparatus 10 using a multi-function dispensing unit according to an embodiment of the present invention.
- the nucleic acid automatic processing apparatus 10 is roughly provided in the multifunctional dispensing unit 11, the temperature controller 60, the measurement unit 54, the multifunctional dispensing unit 11, and the multifunctional dispensing unit 11.
- CPU + program 70 including a CPU, a ROM, a RAM, various external memories, a communication function such as a LAN, a program stored in the ROM, and the like for performing various controls on the temperature controller 60 and the measurement unit 54, and a liquid crystal display And an operation panel 19 having an operation section such as a touch panel.
- the multi-function dispensing unit 11 includes one or more suction / discharge mechanisms 50 for sucking and discharging gas, and one or two or more detachable mounting tips 26 that can suck and discharge liquid by the suction / discharge mechanism.
- a nozzle head 16 having a nozzle 18, a container group 20 having at least one or two or more liquid storage units and one or two or more reaction containers 22 for storing an amplification solution 33 used for nucleic acid amplification; And a moving mechanism 40 that can move with respect to the group 20.
- the nozzle head 16 has an identification information reading unit 55 such as a digital camera provided on the nozzle head 16 for reading the identification information displayed on the identification information display units 28 and 36 of the container group 20 described later. Have.
- the temperature controller 60 determines the temperature of the reaction container in the reaction container group 22 of the container group 20 that contains the liquid to be subjected to temperature control, and a later-described nucleic acid processing control unit 72 provided in the CPU + program 70. It has a temperature source that can be raised or lowered based on an instruction from.
- the measurement unit 54 can measure an optical state including light emission, coloration, color change, or light change occurring in the reaction container, and the measurement end 52 that receives light based on the light emission or the like is provided with the multi-function dispensing.
- the unit 11 is provided.
- the nozzle head 16 can be moved in the X-axis direction and the Y-axis direction with respect to the container group 20 by the moving mechanism 40, and the nozzle head 16 is moved in the Z-axis direction by the moving mechanism 40.
- the magnetic end 15 and the measurement end 52 are capable of applying a magnetic force to and removing the magnetic end.
- the nozzle 18 is provided with a heating unit 51 that heats a light-transmitting sealing lid 30 that seals the amplification solution 33 contained in the reaction vessel 22 and prevents condensation on the sealing lid 30.
- a heating unit 51 that heats a light-transmitting sealing lid 30 that seals the amplification solution 33 contained in the reaction vessel 22 and prevents condensation on the sealing lid 30.
- the container group 20 includes a liquid storage unit group 24 composed of a plurality of storage units that can store or store liquids, and a plurality of units that can store or store instruments used by being mounted on the nozzles 18 of the multi-function dispensing unit 11. It consists of one or two or more storage part series which combined and paralleled the instrument storage part group 21 which consists of this storage part.
- the liquid container group 24 includes, in addition to the reaction container group 22, one or two or more liquid container parts that contain at least the magnetic particle suspension 31, and separation and extraction used for separation and extraction of nucleic acids or fragments thereof.
- Identification information for identifying the liquid storage unit group is displayed on the identification information display unit 36 in the liquid storage unit group 24, and identification information for identifying the device storage unit group 21 is displayed in the device storage unit group 21. It is displayed on the identification information display unit 28.
- the CPU + program 70 gives instructions for a series of processes such as extraction, amplification, sealing of the amplification solution, measurement of optical state such as luminescence generated in the amplification solution, etc., for the nucleic acid or a fragment thereof.
- Various control units 76 that control the suction / discharge mechanism 50 and the moving mechanism are included.
- the nucleic acid processing control unit 72 instructs the moving mechanism 40, the suction / discharge mechanism 50 of the nozzle head 16, the desorption mechanism 53, and the magnetic force unit 15 to perform a series of processing for extracting the nucleic acid or a fragment thereof. Sealing that instructs the extraction control unit 73, the moving mechanism 40, the suction / discharge mechanism 50, or the desorption mechanism 53, and the heating unit 51 to seal the amplification solution on the reaction vessel 22 A control unit 74 and a measurement control unit 75 for instructing measurement to the measurement unit 54 are provided.
- FIG. 2 is a perspective view showing an automatic nucleic acid processing apparatus 101 using the multi-function dispensing unit according to the first embodiment.
- FIG. 2 (a) shows the appearance of the nucleic acid automatic processing apparatus 101, and a housing 14 in which a multi-function dispensing unit 111 corresponding to the multi-function dispensing unit 11 is built,
- a door 12 that covers the opening of the housing 14 so as to be openable and closable; an operation panel 191 having a liquid crystal display unit and operation keys provided on the door 12 corresponding to the operation panel 19; and an opening and closing recess 13 of the door 12
- the size of the housing 14 is, for example, about 50 cm in length, about 30 cm in width, and about 40 cm in height.
- FIG. 2B shows a state in which the door 12 is opened, and a multi-function dispensing unit 111 is provided in the housing 14, and the multi-functional dispensing unit 111 is One or two or more (1 in this example) nozzle 181 corresponding to the nozzle 18, a suction / discharge mechanism 501 corresponding to the suction / discharge mechanism 50, and a magnetic force portion 151 corresponding to the magnetic force portion 15 are provided.
- the nozzle head 161 corresponding to the nozzle head 16 and the nozzle group 181 on the stage 17 are provided in a movable region, and correspond to the container group 20 having a reaction container group in which nucleic acid or a fragment thereof is amplified.
- FIG. 3A is a side view showing a Z-axis moving mechanism 401z that moves the nozzle head 161 and the moving mechanism 401 in the Z-axis direction
- FIG. 3B is a perspective view thereof.
- the nozzle head 161 is provided connected to the head base 16a, a head base 16a connected to the head base 16a that is movable in the X, Y, and Z-axis directions with respect to the container group 201, and a dispensing tip 261.
- the Z-axis moving mechanism 401z is a mechanism that allows the head base portion 16a and members connected thereto to move in the Z-axis direction with respect to an XY-axis moving body 41 described later, and includes a Z-axis motor 42a and the Z-axis moving mechanism 401z.
- a ball screw 42 rotated by a motor 42a, a Z-axis drive plate 43 screwed with the ball screw 42 and driven along the Z-axis direction by the rotation of the ball screw, and the Z-axis motor 42a are mounted.
- an XY axis moving body 41 that supports the ball screw 42 and is movable along the X axis direction and the Y axis direction by an X axis moving mechanism 401x and a Y axis moving mechanism 401y described later.
- a magnetic part 151 having a magnet 15a provided so as to be able to come in contact with and separate from the separation tip (dispensing tip) 262 attached to the nozzle 181 is provided below the XY-axis moving body 41.
- a magnetic part 151 having a magnet 15a provided so as to be able to come in contact with and separate from the separation tip (dispensing tip) 262 attached to the nozzle 181 is provided.
- the separation chip 262 is positioned at a predetermined height, it is possible to apply a magnetic force to the inside of the separation chip 262.
- the dispensing tip 261 includes a mouth portion 261a through which the liquid can be flowed in and out by the suction / discharge mechanism 501, and a small-diameter tube 261b provided with the mouth portion 261a at the tip.
- a large-diameter pipe 261c communicating with the small-diameter pipe 261b and formed thicker than the small-diameter pipe 261b, and a mounting opening 261d provided at the end of the large-diameter pipe 261c and attached to the nozzle 181 Have As will be described later, the separation tip 262 has a capacity larger than the dispensing tip 261 and is about 1 mL.
- the suction / discharge mechanism 501 includes a P-axis motor 501a, one end connected to the P-axis motor 501a, rotationally driven by the P-axis motor 501a, and the other end supported by a ball screw 501b pivotally supported by the head base 16a.
- a P-axis drive plate 501c that is screwed with the ball screw 501b and moves up and down along the Z-axis direction by the rotation of the ball screw 501b, and a flow pipe in the nozzle 181 and a vent pipe 181d communicate with each other.
- a suction / discharge mechanism 501d provided with a slidable piston, and a piston rod 501e provided with the piston at one end and connected to the P-axis drive plate 501c at the other end.
- the magnetic part 151 includes a magnet 15a provided so as to be able to contact and separate from the small-diameter tube 262b of the dispensing tip 262 moved to a predetermined height position, and supports the magnet 15a at one end and the other end is fixed.
- reference numeral 551 denotes a digital camera as an identification information reading unit 55 for reading identification information displayed on identification information display units 281 and 361 (to be described later) of the container group 201.
- the detaching mechanism 531 provided in the nozzle head 161 is provided through the bottom plate 16b connected to the head base portion 16a and the P-axis of the suction / discharge mechanism 501.
- Two injection pins 53a which are pressed by the lowering of the drive plate 501c and can move downward, are connected at the lower ends of the injection pins 53a and are provided below the bottom plate 16b and the nozzles.
- a chip removal which is provided with a hole 53b which surrounds 181 and is movable in the axial direction and which is larger than the nozzle 181 but has a smaller inner diameter than the largest outer diameter of each of the chips 261, 262 and 271.
- a plate 53c which is provided with a hole 53b which surrounds 181 and is movable in the axial direction and which is larger than the nozzle 181 but has a smaller inner diameter than the largest outer diameter of each of the chips 261, 262 and 271.
- a plate 53c which is provided with a hole 53b which surrounds 181 and
- the detaching mechanism 531 is provided at the upper end of the injection pin 53a, and one end is attached to the head 53d contacting the P-axis drive plate 501c and the bottom plate 16b, and surrounds the injection pin 53a.
- the measurement unit 541 is incorporated in the nozzle head 161 as a whole, and a part of the measurement unit 541 is formed in the nozzle 181 and can move together with the nozzle 181.
- the unit 541b is attached to the head base portion 16a and optically connected to the nozzle 181 through optical fibers 541c, 541d, 541e, and 541f, and interlocks with the nozzle 181 and the nozzle head 161.
- FIG. 4 shows the nozzle head 161, the X-axis moving mechanism 401x, and the Y-axis moving mechanism 401y according to the first embodiment.
- the X-axis moving mechanism 401x includes an X-axis motor 46a provided on a Y-axis drive plate 45 driven by a Y-axis moving mechanism 401y described later, and the X-axis motor 46a.
- a pulley 46b that is rotationally driven, a pulley 46c that is pivotally supported by the Y-axis drive plate 45, a timing belt 46 that spans between the two pulleys 46b and 46c, and the XY-axis moving body 41 are connected to each other.
- the timing belt 46 includes an X-axis drive plate 44 that can move along the X-axis direction, and a guide rail 44 a that guides the X-axis drive plate 44 along the X-axis direction of the Y-axis drive plate 45. .
- Two shafts 48 which will be described later, pass through the two holes 47 formed in the Y-axis drive plate 45, and the Y-axis drive plate 45 is guided by the shafts 48 along the Y-axis direction. It is movable.
- the Y-axis moving mechanism 401y includes two shafts 48 supported at both ends by shaft fixing blocks 48a and 48b supported by the main body of the multi-function dispensing unit 111, Similar to the fixed block 48b, a Y-axis motor 49a supported by the main body of the multi-function dispensing unit 111, a pulley 49b rotated by the Y-axis motor 49a, and a pulley 49c supported by the main body, A timing belt 49 spanned between the two pulleys 49b and 49c, and the Y-axis drive plate 45 driven in the Y-axis direction by the timing belt 49 are provided.
- FIG. 5 shows the nozzle 181 of the nucleic acid automatic processing apparatus 101 shown in FIGS.
- the nozzle 181 can be mounted by detachably fitting a cavity 181a provided therein, a dispensing tip 261, a separating tip 262, and a drilling tip 271, and changes in the optical state of the light emission and the like.
- the lower end 181c as the tip portion provided with the measurement end 521 for receiving the light based on the above, the upper portion 181b having an outer diameter larger than the lower portion 181c, and provided so as to protrude sideways from the side surface of the upper portion 181b.
- a vent pipe 181d that communicates with the cavity 181a and allows gas to pass through the interior, and an opening at the lower end of the cavity 181a, and is provided at three locations for communicating the cavity 181a with the outside.
- the gaps 181e, 181f, and 181g have a plug 181h formed between the openings.
- the nozzle 181 further includes a heating unit 511 provided so as to surround the outer peripheral surface of the upper portion 181b, and a part of the measurement unit 541 including the measurement end 521 therein.
- a heating unit 511 provided so as to surround the outer peripheral surface of the upper portion 181b, and a part of the measurement unit 541 including the measurement end 521 therein.
- tips 521c, 521d, and 521e are exposed at the lower end surface of the plug 181h, penetrate the nozzle 181 through the cavity 181a of the nozzle 181 along the axial direction of the nozzle 181, and penetrate the nozzle 181.
- irradiation optical fibers 541c, 541d, and 541e arranged so as to form a central angle of about 120 degrees with respect to the axis of the nozzle 181 and the tip 521f are exposed at the lower end surface of the plug 181h, and the axis of the nozzle 181 And a light receiving optical fiber 541f penetrating through the cavity 181a.
- the other end of the irradiation optical fiber 541c-541e is connected to, for example, a light source irradiation unit 541b incorporating three types of light sources, and the other end of the light receiving optical fiber 541f is connected to the light receiving unit 541a.
- selection means including a plurality of filters for selecting the wavelength or wavelength band of the light input from the light receiving optical fiber 541f. According to this embodiment, a plurality of types of fluorescent substances can be identified.
- FIG. 6 shows a state at the time of measurement of real-time PCR.
- the sealing lid 301 is attached to the lower portion 181c of the nozzle 181 to close the opening of the wide-mouthed tube portion 221b of the reaction vessel 221 of the vessel group 201.
- the amplification solution 33 is accommodated in the narrow tube portion 221 a of the reaction vessel 221 and sealed in the reaction vessel 221 by the sealing lid 301.
- the temperature controller 601 is provided below the reaction vessel 221.
- the temperature controller 601 includes a heat block 601a having a recess shaped to fit with the narrow tube portion 221a of the reaction vessel 221, a Peltier element 601b capable of heating and cooling the heat block 601a, and a heat sink 601c. is doing.
- the entire measurement unit 541 is incorporated in the nozzle head 161 and interlocked with the nozzle 181 so that the optical fiber 541c, 541d, 541e, 541f is moved by the movement of the nozzle 181. Since the parts of the measurement unit 541 are not deformed or fluctuate mutually, the life of the apparatus is long.
- FIG. 7 shows the nucleic acid or fragment thereof in a state where the reaction vessel is closed during the real-time PCR measurement and the amplification solution 33 is sealed in the reaction vessel.
- FIG. 7A shows a state where the amplification solution 33 contained in the reaction vessel 220 is sealed using only the sealing liquid 29.
- the opening of the reaction vessel 220 is narrow and the surface tension of the sealing liquid 29 is smaller than that of water or the material of the vessel, a curved liquid surface having a relatively large curvature is formed. Therefore, when measuring an optical state such as light emission in the amplification solution 33 from above the sealing liquid 29, light is scattered due to the curved liquid surface, and clear and highly accurate measurement is performed. May not be possible. Therefore, in this case, it is preferable to measure the optical state from the side surface of the reaction vessel 220.
- FIG. 7B shows a state where the amplification solution 33 accommodated in the reaction vessel 221 is sealed using only the sealing liquid 29.
- the amplification solution 33 is a liquid amount stored only in the narrow tube portion 221a of the reaction vessel 221.
- the amount of liquid that reaches the wide-mouthed pipe portion 221b is used as the sealing liquid 29.
- the sealing liquid 29 spreads over a wide opening area of the wide-mouthed pipe portion 221b, the influence of the surface tension with the reaction vessel wall surface becomes relatively small, and a substantially flat liquid surface is obtained.
- the measurement using the nozzle 183 above the reaction vessel 221 can be performed with clear and high accuracy.
- the temperature controller 601 is provided below the reaction vessel 221.
- FIG. 7C shows a case where the amplification solution 33 stored in the reaction vessel 221 is sealed with the amplification solution 33 stored in the narrow tube portion 221a using both the sealing liquid 29 and the sealing lid 301.
- the bottom surface 301b of the sealing lid 301 is shown in a case where the bottom surface 301b is sealed in contact with the sealing liquid 29 via an air layer without contacting the sealing liquid 29.
- FIG. 7D shows a state in which the amplification solution 33 accommodated in the reaction vessel 221 is sealed with the amplification solution 33 accommodated in the narrow tube portion 221 a using both the sealing liquid and the sealing lid 302. .
- the bottom surface 302 a of the sealing lid 302 is translucent like the sealing lid 301.
- the central portion is recessed downward. Accordingly, in the sealed state, the sealing liquid 29 is in contact with the sealing liquid 29, and no air layer is formed between the sealing liquid 29 and the central portion of the bottom surface 302a.
- the light from the amplification solution 33 transmits clear light by removing the influence of the boundary surface between the sealing liquid 29 and the air layer, for example, the influence of scattering, refraction, reflection and the like due to surface tension. Can do.
- FIG. 8 is an enlarged plan view showing a container section 201 of a container group 201 as a first embodiment example of the container group 20 provided on the stage 17 of the multi-function dispensing unit 111.
- the container group 201 is composed of two cartridge containers 241 and 211 whose longitudinal direction is provided along the Y-axis direction and arranged in parallel along the X-axis direction.
- the cartridge container 241 is a reaction container as a liquid container unit group. And the liquid container are arranged in a row, and the cartridge container 211 is arranged in a row with various instruments used by being attached to the nozzle 181 of the multifunctional dispensing unit 111 as an instrument container group. .
- the cartridge container 241 includes four reaction containers 221 for PCR amplification as the reaction container group 22, two reaction containers 222 maintained at a predetermined temperature by a constant temperature controller 611 described later, and 10
- a reagent storage unit group 231 including four liquid storage units and a liquid storage unit group 232 including four tubes are provided.
- the volume of the reaction vessel 221 is about 200 ⁇ L, and the volume of each of the other reaction vessels, each liquid container, and the tube is about 2 mL.
- the reaction vessel 221 is used for amplification of a nucleic acid or a fragment thereof, and temperature control is performed by the temperature controller 601 based on a predetermined amplification method.
- the reaction vessel 221 is formed in two stages as shown in FIG. 6 and is provided on the lower side and is connected to the narrow tube part 221a provided on the upper side and the narrow tube part 221a provided on the upper side.
- a wide opening 221b having an opening wider than the opening of the thin tube 221a and accommodating the sealing liquid 29.
- the four reaction vessels 221 are covered with a film 221c that can be peeled by the user's hand to prevent contamination of the reaction vessels 221.
- the inner diameter of the wide-mouthed tube portion 221b is, for example, 8 mm
- the inner diameter of the opening portion of the narrow tube portion 221a is, for example, about 5 mm.
- the reagent storage unit group 231 stores seven types of separation / extraction solutions 32 as follows.
- a liquid storage part 231J containing 1.2 mL of distilled water, a liquid storage part 231G containing a dissociation liquid, a liquid storage part 231F containing 700 ⁇ L of cleaning liquid 2, a liquid storage part 231E containing 700 ⁇ L of cleaning liquid 1, and 500 ⁇ L of binding buffer liquid
- the openings of the ten reagent storage unit groups 231 are pre-packed with the respective reagents and the like by covering with a pierceable film 231a.
- a tube 232B containing 70 ⁇ L of Green Mix and an empty tube that can contain the extracted nucleic acid or fragment thereof 232A.
- a QR (registered trademark) code as identification information is attached to an identification information display unit 281.
- the identification information consists of sample information and test information, and the sample information consists of the name, ID number, or collection date of the patient from whom the sample was collected, and the test information is used to specify a test item, for example, an influenza virus. Genetic diagnosis, the type of reagent to be tested, and the reagent production lot number.
- the cartridge container 241 integrally includes a reaction container group 221, a reagent storage unit group 231 and a substrate 241a, and each tube of the liquid storage unit group 232 is attached to and detached from five holes formed in the substrate 241a. Held possible.
- the cartridge container 211 has an accommodating part 211G for accommodating a sealing lid 301 that can be attached to the lower part 181c of the nozzle 181 for sealing the amplification solution 33 accommodated by closing the opening of the reaction container 221.
- Four chip accommodating portions 211C, 211D, 211E, 211F accommodating a dispensing tip 261 having a capacity of about 200 ⁇ L, and a chip accommodating portion accommodating a separation tip 262 having a capacity of about 1 mL as a kind of dispensing tip 211B and a chip accommodating portion 211A that accommodates a perforating chip 271 capable of perforating the film 231a.
- the openings of these accommodating portions 211A-211G are covered with a film 211b, and the sealing lid 301, the four dispensing tips 261, the separating tips 262, and the punching tips 271 are previously sealed in these accommodating portions. ing.
- the film 211b can be peeled from the substrate 211a by the user's hand.
- the substrate 211a of the cartridge container 211 and the accommodating portions 211A-211G are integrally formed.
- no container is provided in a part adjacent to the part of the cartridge container 241 arranged in parallel with the reaction container group 221 in the X-axis direction.
- a tip is attached to the nozzle when the measurement is performed on the reaction vessel 221 at the measurement end. This is to avoid mounting or contact between the nozzle and the tip.
- an accommodating portion for accommodating a sealing lid 301 for sealing each reaction vessel 221 may be provided in a portion adjacent to the portion provided with the reaction vessel group 221 in the X-axis direction. good. In this case, the sealing lid 301 is attached to the reaction vessel 221 and the accommodating portion is empty.
- FIG. 9 shows an automatic nucleic acid processing apparatus 102 using a multi-function dispensing unit according to the second embodiment.
- the nucleic acid automatic processing apparatus 102 using the multi-function dispensing unit has a pipe 502a as a nozzle 182 through which a flow path 182a through which gas can pass and a suction / discharge mechanism 50 for sucking and discharging gas are used.
- a cylinder 502b that is connected to the flow path 182a of the nozzle 182 and in which a piston can slide, a piston rod 502c provided with a piston 502d at one end, and the sealing lid 301 attached to the nozzle 182.
- a measuring unit 542 capable of measuring an optical state including light emission, coloration, color change, and light change generated in the amplification solution sealed in the reaction vessel 221. .
- a dispensing tip 261, a separating tip 262, and a perforating tip 271 are detachably attached to the lower portion 182c of the nozzle 182, and the lower end thereof is light based on a change in the optical state such as the light emission. Corresponds to the measurement end 522 that receives light.
- An upper portion 182b having an outer diameter larger than that of the lower portion 182c is provided on the upper side of the nozzle 182, and the heating unit 511 is provided along an outer peripheral surface of the upper portion 182b near the lower portion 182c, and the lower portion 182c.
- the sealing lid 301 attached to the is heated to prevent condensation on the sealing lid 301.
- a light receiving optical fiber 542 c and an irradiation optical fiber 542 d extend from a lower portion 182 c as the tip portion to a gap portion 182 e provided in the middle of the upper portion 182 b along the axis of the nozzle 182.
- the flow path 182a is provided at a position where it does not pass through the gap 182e.
- the other ends of the light receiving optical fiber 542c and the irradiation optical fiber 542d are connected to the rod lens 542e inside the lower portion 182c of the nozzle 182 to form a measuring end 522.
- FIG. 10 shows a state in which the dispensing tip 261 and the separation tip 262 are mounted on the nozzle 182 of the nucleic acid automatic processing apparatus 102 using the multifunction dispensing unit according to the second embodiment.
- a cartridge container 202 is provided as a container group 20 on the stage of the apparatus 102.
- the cartridge container 202 has a plurality of holes formed in the substrate 202a, and a liquid storage part group 202b composed of a reagent storage part group 234 and a reaction container 222 integrally formed in each hole, separately from each other.
- the formed reaction vessel 221 and the sealed lid 301 formed separately are held so as to be fitted.
- the reaction vessel 221 from the right side, a sealing lid 301 accommodated in the reaction vessel 221, a reaction vessel group 222 consisting of two reaction vessels, and a reagent storage portion group 234 consisting of ten liquid storage portions.
- the fitting is held in each hole.
- the reaction vessel 221 is formed of a two-stage narrow tube portion 221a and a wide-mouth tube portion 221b.
- a heat block 601a of a temperature controller 601 is provided below the thin tube portion 221a of the reaction vessel 221, and the amplification solution sealed through the heat block 601a fitted to the outer bottom portion of the thin tube portion 221a. Temperature control is performed.
- a sealing lid 301 for sealing the amplification solution accommodated in the reaction vessel 221 is held so as to fit into a sealing lid holding hole 251 formed in the substrate 202 a, and is attached to the outer peripheral side surface of the sealing lid 301.
- An annular protrusion 301a is provided along the surface to enhance the sealing performance when fitted to the wide-mouthed tube portion 221b of the reaction vessel 221.
- At least the bottom surface 301 b of the sealing lid 301 has translucency, and the optical state in the reaction vessel 221 can be measured via the sealing lid 301.
- the concave portion on the upper side of the sealing lid 301 is a fitting portion 301c into which the lower portion 182c of the nozzle 182 can be fitted.
- reaction container group 222 and the reagent storage unit group 234 of the liquid storage unit group 202b are covered with a prepack film 202c to prevent evaporation and contamination of the liquid.
- a heat block of a constant temperature controller 611 is provided below the reaction container group 222, and temperature control is performed through the heat block fitted to the outer bottom of each liquid container of the reaction container group 222.
- the separation chip 262 attached to the nozzle 182 sucks the magnetic particle suspension 31 as shown in FIG. 10B while moving along the Y-axis direction which is the longitudinal direction of the cartridge container 202. Then, a magnetic field is applied to the small-diameter tube by the magnet 15a to attract the magnetic particles to the inner wall, and the processing is performed while moving to the next liquid storage unit.
- FIG. 11A shows the above-described sealing lid 301 accommodated in the sealing lid holding hole 251 of the cartridge container 202 which is the container group shown in FIG.
- the nozzle 182 is lowered by the Z-axis movement mechanism, so that the sealing lid 301 is fitted and attached to the lower part 182 c of the nozzle 182, and then the Y-axis movement mechanism This shows a state where the container group 202 is moved in the Y-axis direction and positioned on the reaction container 221.
- FIG. 11B shows a measurement state of real-time PCR, and the sealing lid 301 is lowered by lowering the nozzle 182 equipped with the sealing lid 301 toward the reaction vessel 221 by a Z-axis moving mechanism.
- the amplification solution 33 accommodated in the narrow tube portion 221a of the reaction vessel 221 is sealed by fitting it to the wide-mouthed tube portion 221b of the reaction vessel 221.
- the narrow tube portion 221a is fitted into the recess of the heat block 601a and the temperature is controlled.
- the measurement end 522 of the measurement unit 542 can receive light based on the change in the optical state in the reaction vessel 221 so that condensation does not occur on the bottom surface 301b of the sealing lid 301.
- the heating unit 511 is heated.
- the narrow tube portion 221a is fitted into the recess of the heat block 601a and the temperature is controlled.
- the measurement end 522 of the measurement unit 542 can receive light based on the change in the optical state in the reaction vessel 221 so that condensation does not occur on the bottom surface 301b of the sealing lid 301.
- the heating unit 511 is heated.
- the flow path 182a and the optical fibers 542c and 542d are provided so as not to contact with each other, the influence of the fluid on the optical fiber can be ignored. Further, by providing the rod lens 542e, a wide range of optical states can be measured.
- FIG. 12 shows a cartridge container 203 according to a third embodiment corresponding to the container group 20 placed on the stage 17 of the automatic nucleic acid processing apparatus 101 using the multifunctional dispensing unit shown in FIG. Show.
- the cartridge container 203 is formed by integrally molding the substrate 203a and each accommodating portion.
- a sealing lid for sealing the reaction vessel 221 from the right side and the amplification solution 33 stored by closing the opening of the reaction vessel 221 in the reaction vessel 221.
- a sealed lid accommodating portion 252 containing 301 a reaction container group 224 composed of two liquid accommodating portions, and a reagent accommodating portion group 236 composed of ten liquid accommodating portions.
- the opening of the sealing lid accommodating portion 252 that accommodates the reaction vessel 221 and the sealing lid 301 is covered with a prepack film 203d that can be peeled off by the user's hand, and the reaction vessel group 224 and the reagent etc. accommodating portion group.
- the opening 236 is covered with a prepack film 203 b that can be punched by a punching tip 271.
- FIG. 12B shows a state before peeling off the film 203d
- FIG. 12C shows a state after peeling. Note that the end 203c of the film 203d is not attached to the substrate 203a so as to be easily peeled off.
- FIG. 13 shows an automatic nucleic acid processing apparatus 103 using a multi-function dispensing unit according to the fourth embodiment.
- FIG. 13A shows the appearance of the nucleic acid automatic processing apparatus 103, which is the same as the apparatus 101 according to the first embodiment, but the contents and operations displayed on the operation panel 192 are as follows. Different. Here, the same components as those in the first embodiment are denoted by the same reference numerals and the description thereof is omitted.
- FIG. 13B shows a state in which the door 12 is opened.
- a multi-function dispensing unit 113 is provided in the housing 14, and the multi-functional dispensing unit 113 is The nozzle 183 corresponding to the nozzle 18 of 1 or 2 (1 in this example), the suction / discharge mechanism 503 corresponding to the suction / discharge mechanism 50, and the magnetic part 151 corresponding to the magnetic part 15 are provided.
- FIG. 14 shows an X-axis moving mechanism 401x for moving the nozzle head 163 and the moving mechanism 401 in the X-axis direction and a Z-axis moving mechanism 401z for moving in the Z-axis direction.
- the Y-axis moving mechanism 401y that moves is the same as that shown in FIG.
- a nozzle head 163 is provided on a head base 163a that can move in the X, Y, and Z-axis directions with respect to the container group 201, and a bottom plate 163b that is connected to the head base 163a.
- a nozzle 183 that can be mounted in each mounting opening 261d of the chip 261, the separation chip 262, and the drilling chip 271 (see FIG. 10), and the flow path of the nozzle 183 communicate with the air through the nozzle 183.
- a suction / discharge mechanism 503 capable of sucking and discharging the liquid, a detachment mechanism 531 provided on the head base 163a, which enables the detachment of a dispensing tip 261 attached to the tip of the nozzle 183, and the head base 163a.
- a measuring end 523 which is attached as a whole so as to interlock with the nozzle 183 and receives light based on the change in the optical state is provided on the nozzle 18.
- the “predetermined interval” is, for example, a length shorter than the distance between the centers of the widths of the cartridge container 241 and the cartridge container 211 shown in FIG. Length.
- the suction / discharge mechanism 503 has a P-axis motor 503a, one end connected to the P-axis motor 503a, rotationally driven by the P-axis motor 503a, and the other end pivotally supported by the head base 163a; A P-axis drive plate 503c that is screwed with the ball screw 503b and moves up and down along the Z-axis direction by the rotation of the ball screw 503b; and the nozzle 183 directly communicates with the inside so that the piston can slide. And a piston rod 503e connected to the P-axis drive plate 503c at the other end. In the nozzle 183, a flow pipe communicating with the cylinder 503d is provided along the axial direction.
- the measuring unit 543 is attached to the bottom plate 163b of the nozzle head base 163a as a whole so as to interlock with the nozzle 183.
- the measuring unit 543 includes a dark box 543j and a lower side from the dark box 543j. It has a light guide tube 543a that protrudes and allows light to pass therethrough and is provided with the measurement end 523 at the lower end.
- an irradiation unit 543c for irradiating excitation light provided at the upper end of the light guide tube 543a, a branch tube 543k branched to the side of the light guide tube 543a, and the branch tube 543k
- a light receiving unit 543d that is optically connected to the light guide tube 543a via the light source, and reflects only light having a predetermined wavelength among light incident on the measurement end 523 provided in the light guide tube 543a.
- a filter plate drive plate 543f that is connected to the filter plate 543e and can be moved up and down along the Z-axis direction, and is screwed with the drive plate 543f to move the drive plate 543f up and down.
- a reaction vessel 221 including a narrow tube portion 221a for storing an amplification solution and a wide-mouth tube portion 221b for storing the translucent sealing liquid 29 is located below the measurement end 523. Is sealed in the reaction vessel 221 by the sealing liquid 29.
- the measuring unit 543 is provided with selection means including a plurality of filters that can select the wavelength or wavelength band of the received light. A fluorescent substance can be identified.
- the entire measurement unit 543 is incorporated in the nozzle head 163 and interlocked with the nozzle 181. Accordingly, since the measuring unit 543 is not deformed by the movement of the nozzle 183, the life of the apparatus is long.
- FIG. 16 shows a measurement unit 544 according to the fifth embodiment. Similar to the measurement unit 543 according to the fourth embodiment, the measurement unit 544 is attached to the nozzle head 163 as a whole so as to interlock with the nozzle 183, and emits light and colors generated in the reaction vessel. An optical state including color change or light change can be measured, and a measurement end 524 that receives light based on the light emission or the like is separated from the tip end portion of the nozzle 183 along the X-axis direction at a predetermined interval. Provided on the lower side of the bottom plate 163b.
- the measurement unit 544 includes a dark box 544j and a light guide tube 544a that protrudes downward from the dark box 544j, allows light to pass therethrough, and is provided with the measurement end 524 at the lower end.
- a light receiving unit 544d provided so as to be connected at the upper end of the light guide tube 544a, and a predetermined wavelength of light incident from the measurement end 524 provided in the light guide tube 544a.
- the dichroic mirror 544b that reflects only the light it has is guided to the branch tube 544i and transmits light of other wavelengths, and the excitation light connected to the light guide tube 544a is irradiated through the branch tube 544i.
- FIG. 17 shows a measuring unit 545 according to the sixth embodiment. Similar to the measurement unit 543 according to the fourth embodiment and the measurement unit 544 according to the fifth embodiment, the measurement unit 545 is attached to the nozzle head 163 as a whole so as to interlock with the nozzle 183.
- the optical state including light emission, coloration, color change or light change occurring in the reaction vessel can be measured, and a measurement end 525 for receiving light based on the light emission or the like is connected to the tip end of the nozzle 183 and X
- the bottom plate 163b is connected to the head base 163a at a predetermined interval along the axial direction.
- the measurement unit 545 includes a dark box (not shown) as shown in FIG. 15 or 16, and a light guide tube 545a protruding downward from the dark box and allowing light to pass therethrough and having the measurement end 525 at the lower end.
- a dark box (not shown) as shown in FIG. 15 or 16
- a light guide tube 545a protruding downward from the dark box and allowing light to pass therethrough and having the measurement end 525 at the lower end.
- an irradiation unit 545c is provided at the upper end of the light guide tube 545a for irradiating excitation light optically connected to the light guide tube 545a, and branches to the side of the light guide tube 545a.
- a filter plate 545e a filter plate drive plate 545f connected to the filter plate 545e and capable of moving up and down along the Z-axis direction, a ball screw 545g screwed with the drive plate 545f and moved up and down by rotation.
- FIG. 18 (a) shows the appearance of the nucleic acid automatic processing apparatus 104, and a housing 141 in which a multifunctional dispensing unit 114 corresponding to the multifunctional dispensing unit 11 is built, A door 121 that covers the opening of the housing 141 so as to be openable / closable, an operation panel 193 having a liquid crystal display portion and operation keys provided on the door 121 corresponding to the operation panel, and an opening / closing recess 131 of the door 12.
- a door 121 that covers the opening of the housing 141 so as to be openable / closable
- an operation panel 193 having a liquid crystal display portion and operation keys provided on the door 121 corresponding to the operation panel
- FIG. 18B shows a state in which the door 121 is opened.
- a multi-function dispensing unit 114 is provided inside the housing 141, and the multi-functional dispensing unit 114 is Four nozzles 183 (183i, 183ii, 183iii, 183iv), four cylinders (503), and a small-diameter tube of a separation tip 262 attached to the four nozzles 183 corresponding to the magnetic force section 15.
- a reaction vessel group for amplifying nucleic acid or a fragment thereof is applied to the nozzle head 164 corresponding to the nozzle head 16 provided with the magnetic force portion 152 capable of exerting a magnetic force all at once and the four nozzles 183.
- the four container groups 201 having, and a moving mechanism 402 corresponding to the moving mechanism 40 that allows the four nozzles to move in the X-axis, Y-axis, and Z-axis directions with respect to each container group 201.
- four measuring units 543 capable of measuring an optical state including light emission, coloration, color change, or light change generated in a reaction vessel provided in the nozzle group 164 and provided in the vessel group 201. .
- FIG. 19 shows four nozzles 183 (183i, 183ii, 183iii, 183iv) provided in the nozzle head 164 for the four container groups 201 (201i, 201ii, 201iii, 201iv) provided on the stage 17. And the positional relationship by the movement of the four measurement parts 543 (543i, 543ii, 543iii, 543iv) is shown.
- Each nozzle 183i, 183ii, 183iii, 183iv is set on the stage 17 with a dedicated area 17i corresponding to the nozzle 183i into which one nozzle, for example, the nozzle 183i enters and the other nozzles 183ii, 183iii, 183iv do not enter.
- the corresponding dedicated areas 17ii, 17iii, and 17iv are set for the other nozzles 183ii, 183iii, and 183iv.
- the container groups 201i, 201ii, 201iii, 201iii, 201iv are provided in the dedicated areas 17i, 17ii, 17iii, 17iv, respectively, and the measurement ends 523i, 523ii, 523iii, 523iv belonging to the measurement units 543 are connected to the nozzle head.
- 164 provided at predetermined intervals along the longitudinal direction of the nozzle head 164, that is, the X-axis direction, from the nozzles 183i, 183ii, 183iii, and 183iv, and are included in the dedicated areas in the same manner as the nozzles. In conjunction with the nozzle.
- the “predetermined interval” refers to the cartridge containers 241 i, 241 ii, 241 iii, 241 iv of the liquid container groups belonging to the container groups 201 i, 201 ii, 201 iii, 201 iv, and the cartridge containers corresponding to the adjacent instrument container groups. This is equal to or smaller than the pitch interval between 211i, 211ii, 211iii, and 211iv and larger than the radius of the tip mounted on the reaction vessel or the nozzle.
- FIG. 19A is the maximum X coordinate position in the X-axis direction or the leftmost end of each dedicated area
- FIG. 19B is the minimum X coordinate position in the X-axis direction or the rightmost end of each dedicated area.
- These are the minimum Y coordinate position in the Y axis direction or the uppermost end of each dedicated area
- FIG. 19C is the maximum Y coordinate position in the Y axis direction or the lowermost end of each dedicated area.
- steps S1 to S16 correspond to separation and extraction processing.
- step S 1 the door 12 of the automatic nucleic acid processing apparatus 101 using the multifunction dispensing unit shown in FIG. 2 is opened, and the cartridge container 241 as the container unit 201 as the container group 20 is placed on the stage 17.
- the tubes 232A, 232B, 232C, 232D, and 232E are held in the five holes drilled in the film, and the film 221c that covers the four reaction containers 221 of the cartridge container 241 and each accommodating part of the cartridge container 211 The user peels off the film 211b covering the cartridge, and mounts the cartridge container 241 and the cartridge container 211 in parallel.
- the film 211b is peeled off so that a portion of the film 211b or the film 241c that is not attached to the substrate 211a or 241a of the cartridge container 211 or the cartridge container 241 is provided at the end of the container 211 that does not contain the liquid. If so, the possibility of internal contamination is small (eg, as shown in FIG. 12).
- step S2 after the door 12 is closed, the start of separation and extraction and amplification processing is instructed by operating the touch panel of the operation panel 191 or the like.
- the digital camera 551 serving as the identification information reading unit 55 provided in the nozzle head 161 includes the QR code displayed on the identification information display units 281 and 361 of the cartridge container 241 and the cartridge container 211.
- the identification information is read and analyzed by the identification information analysis unit 71 provided in the CPU + program 70 of the nucleic acid automatic processing apparatus 101, and the correctness of the combination of the container group 201, the purpose of processing, necessary reagents, etc. Match or mismatch is confirmed.
- the extraction control unit 73 provided in the nucleic acid processing control unit 72 of the CPU + program 70 of the apparatus 101 moves the Y-axis movement.
- the mechanism 401y is instructed to move the nozzle head 161 to move in the Y-axis direction to the Y-coordinate position of the accommodating portion 211A in which the piercing tip 271 of the cartridge container 211 is accommodated, and the X-axis moving mechanism 401x is instructed.
- the nozzle 181 is positioned so as to be directly above the accommodating portion 211A.
- the Z-axis moving mechanism 401z is instructed, and the lower portion 181c of the nozzle 181 is lowered to the mounting opening of the drilling tip 271 and fitted and mounted.
- step S5 the nozzle 181 equipped with the perforating tip 271 is moved onto the cartridge container 241 by the X-axis moving mechanism 401x, and is moved by the film 231a along the Y-axis direction using the Y-axis moving mechanism 401y. 10 pieces covered with the film 231a are moved to the liquid containing part 231A of the covered liquid containing part group 231 and drilled by the Z-axis moving mechanism 401z to lower the drilling tip 271 and then lifted again.
- the liquid container 231A-231J and the two reaction vessels 222 are repeatedly drilled.
- step S6 the nozzle 181 is moved again to the piercing tip accommodating portion 211A of the cartridge container 211 by the X-axis moving mechanism 401x, and the P-axis driving plate 501c is lowered to cause the injection of the detaching mechanism 531.
- the pin 53a By lowering the pin 53a, the chip removal plate 53c is lowered, and the drilling chip 271 is detached from the lower part 181c of the nozzle 181 and stored in the chip storage part 211A.
- step S7 the nozzle 181 is moved in the Y-axis direction along the cartridge container 211, and after reaching the accommodating portion 211B, the nozzle 181 is lowered using the Z-axis moving mechanism 401z, thereby the separation chip. 262 is attached to the lower portion 181 c of the nozzle 181. After being lifted by the Z-axis moving mechanism 401z, the separation chip 262 is moved using the Y-axis moving mechanism 401y and the X-axis moving mechanism 401x to reach the liquid container 231J, and then the Z-axis moving mechanism 401z.
- the separation chip 262 is lowered and the P-axis drive plate 501c of the suction / discharge mechanism 501 is lifted to suck 50 ⁇ L from the distilled water stored in the liquid storage portion 231J. Then, the separation chip 262 is again raised above the liquid container 231J, and then the separation chip 262 is moved by the Y-axis moving mechanism 401y so as to be positioned on the liquid container 231H. It is discharged into the liquid storage part 231H and stored as a dissociation liquid. Similarly, 350 ⁇ L of water from the liquid storage part 231J is stored in the liquid storage part 231F.
- step S7 as described above, the solution components (NaCl, SDS solution) previously stored in the liquid storage portion 231C and the liquid storage portion 231E, and the distilled water stored in the liquid storage portion 231F as described above are used.
- a predetermined amount of Isopropanol is sucked from 232C and dispensed into the liquid storage unit 231C, liquid storage unit 231E, and liquid storage unit 231F by a predetermined amount, thereby separating and extracting solution into each liquid storage unit 231C, 231E, 231F.
- binding buffer solution NaCl, SDS, i-Propanol
- washing solution 1 NaCl, SDS, i-Propanol
- washing solution 2 50% water, 50% i-Propanol
- step S8 the separation chip 262 is moved to the tube 232E in which the specimen 35 is accommodated using the Y-axis movement mechanism 401y, and then the tip of the separation chip 262 is moved using the Z-axis movement mechanism 401z.
- the specimen 35 is suspended in the liquid by being inserted into the tube 232E and repeatedly aspirating / discharging the suspension of the specimen 35 using the suction / ejection mechanism 501, and then the specimen suspension is separated. Suction into the tip 262 for use.
- the specimen suspension is moved along the Y-axis by the Y-axis moving mechanism 401y to the liquid storage unit 231A in which Lysis 1 (enzyme) is stored as the separation / extraction solution 32, and the perforated film 231a is moved. Aspiration and discharge are repeated so that the small diameter tube of the separation chip 262 is inserted through the hole and the sample suspension and the lysine lysate 1 are stirred.
- step S9 the entire amount of the agitated liquid is sucked by the separation chip 262 and accommodated in the reaction vessel 222A set to 55 ° C. by the constant temperature controller 611 to perform incubation. Thereby, the protein contained in the specimen 35 is destroyed and the molecular weight is reduced.
- the separation chip 262 is moved to the liquid container 231B by the Y-axis moving mechanism 401y while the reaction liquid remains in the reaction vessel 222A, and the Z-axis moving mechanism 401z and the suction / discharge mechanism 501 is used to suck the entire amount of the liquid stored in the liquid storage unit 231B, and the Y-axis moving mechanism 401y transfers the liquid using the separation chip 262, thereby opening the hole of the film 231a in the reaction vessel 222a.
- the reaction solution is discharged through the small diameter tube.
- step S10 the reaction solution and Lysis 2 (guanidine) as another separation and extraction solution 32 are agitated and incubated in the reaction vessel 222a set at 55 ° C. to solubilize the protein. Dissolve. After a predetermined time, all of the reaction solution is sucked into the separation chip 262, transferred to the liquid storage portion 231C by the Y-axis moving mechanism 401y, and inserted through the hole of the film 231a. Is discharged through.
- Lysis 2 guanidine
- step S11 the binding buffer solution (NaCl, SDS, i-Propanol) as the separation / extraction solution 32 stored in the liquid storage unit 231C and the reaction solution are stirred to further dissolve the solubilized protein. Dehydrated and dispersed nucleic acid or fragment thereof in solution.
- step S12 the separation tip 262 is used to insert the small diameter tube into the liquid storage portion 231C through the hole of the film 231a, and the whole amount is sucked and separated by the Z-axis moving mechanism 401z.
- the chip 262 is raised, the reaction solution is transferred to the liquid storage unit 231D, and the magnetic particle suspension 31 stored in the liquid storage unit 231D and the reaction solution are stirred.
- a cation structure is formed in which Na + ions are bonded to hydroxyl groups formed on the surface of the magnetic particles contained in the magnetic particle suspension 31. Therefore, negatively charged DNA is captured by the magnetic particles.
- step S13 the magnetic particles are adsorbed on the inner wall of the small diameter tube 262b of the separation tip 262 by bringing the magnet 15a of the magnetic force portion 151 close to the small diameter tube 262b of the separation tip 262.
- the magnetic particles are raised by the Z-axis movement mechanism 401z, and the separation tip 262 is moved to the liquid using the Y-axis movement mechanism 401y.
- the thin tube 262b is inserted through the hole of the film 231a by moving from the storage portion 231D to the liquid storage portion 231E.
- the magnetic particles are detached from the inner wall and stirred in the washing liquid 1 to wash the protein.
- the separation tip 262 is moved in a state where the magnetic particles are adsorbed on the inner wall of the small diameter tube 262b by bringing the magnet 15a of the magnetic force portion 151 again close to the small diameter tube 262b of the separation tip 262.
- the Y-axis moving mechanism 401y moves the liquid container 231E to the liquid container 231F by the Z-axis movement mechanism 401z.
- step S14 the thin tube 262b of the separation tip 262 is inserted through the hole of the film 231a using the Z-axis moving mechanism 401z.
- suction and discharge for (i-Propanol) the magnetic particles are stirred in the liquid to remove NaCl and SDS, and the protein is washed.
- the separation tip 262 is moved in a state where the magnetic particles are adsorbed on the inner wall of the small diameter tube 262b by bringing the magnet 15a of the magnetic force portion 151 again close to the small diameter tube 262b of the separation tip 262.
- the Z-axis moving mechanism 401z moves the liquid container 231F from the liquid container 231F to the liquid container 231J containing distilled water by the Y-axis moving mechanism 401y.
- step S15 the small-diameter tube 262b of the separation tip 262 is lowered through the hole by the Z-axis moving mechanism 401z, and the magnetic force is applied to the small-diameter tube 262b of the separation tip 262.
- i-Propanol is replaced with water and removed.
- step S16 the separation tip 262 is moved along the Y-axis direction by the Y-axis moving mechanism 401y, and the small-diameter tube 262b is inserted through the hole of the film 231a into the liquid storage portion 231H.
- Stirring by repeatedly sucking and discharging the magnetic particles in distilled water as the dissociation liquid in a state where the magnet 15a of the magnetic force portion 151 is separated from the small diameter tube 262b of the separation tip 262 and the magnetic force is removed, The nucleic acid or fragment thereof held by the magnetic particles is dissociated (eluted) from the magnetic particles into the liquid.
- the magnet 15a is brought close to the small-diameter tube 262b of the separation chip 262 to apply a magnetic field to the small-diameter tube so that magnetic particles are adsorbed on the inner wall, and the extracted nucleic acid or the like is contained in the liquid storage portion 231H.
- the solution to be left is left.
- the separation chip 262 is moved onto the cartridge container 211 by the X-axis moving mechanism 401x, moved to the accommodating portion 211B by the Y-axis moving mechanism 401y, and the separation in which the magnetic particles are adsorbed from the nozzle 181 by the desorption mechanism 531.
- the chip 262 is desorbed into the housing part 211B together with the magnetic particles.
- step S17 the nozzle 181 is moved along the Y axis by using the Y axis moving mechanism 401y to be positioned above the accommodating portion 211C, and the nozzle 181 is lowered by using the Z axis moving mechanism 401z.
- the nozzle 181 is fitted to the nozzle 181 by being fitted to the mounting opening of the dispensing tip 261 at the lower portion 181 c of the nozzle 181.
- the dispensing tip 261 is moved in the X-axis direction by the X-axis moving mechanism 401x and positioned on the cartridge container 241 and then moved along the Y-axis direction by the Y-axis moving mechanism 401y.
- the dispensing tip 261 is moved onto the cartridge container 211 by the X-axis moving mechanism 401x, the dispensing tip 261 is moved along the Y-axis direction by the Y-axis moving mechanism 401y, and the storage The dispensing tip 261 is attached to and detached from the accommodating part 211C by the attaching / detaching mechanism 531.
- step S18 the nozzle 181 is moved along the Y-axis direction by the Y-axis moving mechanism 401y to be positioned on the accommodating portion 211D, and the lower part 181c of the nozzle 181 is moved down by lowering the Z-axis moving mechanism 401z.
- the dispensing tip 261 is attached to the nozzle 181 by being fitted into the attachment opening of the new dispensing tip 261 accommodated in the accommodating portion 211D.
- the dispensing tip 261 is moved up to the cartridge container 241 by the X-axis moving mechanism 401x and then moved to the tube 232B by the Y-axis moving mechanism 401y, and the master mix ( For example, 40 ⁇ L of SYBR (Green Mix) is sucked and moved along the Y-axis direction using the Y-axis moving mechanism 401 y, and 10 ⁇ L is dispensed into each reaction vessel 221. Thereafter, the dispensing tip 261 is moved to the cartridge container 211 by the X-axis moving mechanism 401x, and further positioned on the accommodating portion 211D by the Y-axis moving mechanism 401y, and then the dispensing tip 261 is removed by the detaching mechanism 531. Are removed from the housing 211D. The extracted solution such as nucleic acid and the master mix are accommodated in the narrow tube portion 221a of each reaction vessel 221.
- step S19 a new dispensing tip 261 accommodated in the accommodating portion 211E is attached to the nozzle 181 in the same procedure as that shown in step S18.
- the dispensing tip 261 is moved onto the cartridge container 241 by the X-axis moving mechanism 401x, and then moved to the tube 232D by the Y-axis moving mechanism 401y, and the sealed liquid contained in the tube 232D.
- each reaction vessel 221A, 221B, 221C, 221D 80 ⁇ L of the mineral oil is sucked and moved along the Y-axis direction using the Y-axis moving mechanism 401y, and 20 ⁇ L is dispensed into each reaction vessel 221A, 221B, 221C, 221D, and each reaction vessel 221A , 221B, 221C, 221D are accommodated so as to reach the wide-mouthed tube portion 221b. Thereafter, the dispensing tip 261 is detached and discarded in the accommodating portion 211E as described above.
- step S20 the nozzle 181 moves to the accommodating portion 211G by the Y-axis moving mechanism 401y, descends by the Z-axis moving mechanism 401z, and the fitting portion of the sealing lid 301 accommodated in the accommodating portion 211G.
- the lower part 181c of the nozzle is fitted and attached to 301c.
- the nozzle 181 is moved to the cartridge container 241 by the X-axis moving mechanism 401x, and is fitted and attached only to the reaction container 221D.
- each of the reaction vessels 221A, 221B, 221C, 221D is first heated at 96 ° C. for 10 minutes by the temperature controller 601.
- the nozzle 181 is detached from the hermetic lid 301 by using the desorption mechanism 531, and is sequentially provided from the upper side of each reaction vessel 221A, 221B, 221C, 221D to the tip of the lower portion 181c of the nozzle 181.
- the change in the optical state based on the light emission generated in each of the reaction vessels 221A, 221B, 221C, 221D is measured via the measurement end 521.
- step S21 a cycle of heating the reaction vessel 221 at 96 degrees for 5 seconds and heating at 60 degrees for 15 seconds is repeated 49 times. At that time, the optical state in the reaction vessel 221 is measured via the measurement end 521 in the same manner as in step S20.
- step S22 the reaction vessel is heated at 74 ° C. for 2 minutes.
- the optical state is measured in the same manner as in steps S20 and S21.
- the measurement can be performed while being reliably closed. Further, dew condensation can be prevented by shaking the sealing lid 301. Further, when the measurement is performed through the sealing lid 301, dew condensation occurring on the sealing lid 301 can be prevented by heating the heating unit 511 provided in the nozzle 181.
- nozzle dispensing tip, separation tip, tip for drilling, container group, container, instrument, measuring end, measuring part, suction / discharge mechanism, moving mechanism, magnetic part, heating part, reaction container, sealing lid, sealing
- the configuration, shape, material, arrangement, quantity, number, and used reagents, specimens, and the like of liquids are not limited to the examples shown in the embodiments.
- the nozzle is moved with respect to the stage, it is also possible to move the stage with respect to the nozzle.
- the present invention is a field that requires treatment, inspection, and analysis of nucleic acids mainly including DNA, RNA, mRNA, rRNA, and tRNA, such as industrial fields, agricultural fields such as food, agricultural products, and marine products processing, and pharmaceutical fields. It relates to the pharmaceutical field, hygiene, insurance, disease, genetics and other medical fields, biochemistry or biology and other physical fields.
- the present invention can be used particularly for processing and analysis for handling various nucleic acids such as PCR and real-time PCR.
Abstract
Description
なお、密閉液のみならず密閉蓋を用いて密閉するのは、温度制御中の測定時、または温度制御や処理終了後に反応容器内の液体が溢れて周辺を汚染しないようにするために好ましいからである。「所定収容部」とは、密閉液であれば液収容部、密閉蓋であれば密閉蓋収容部である。
該核酸自動処理装置10は、大きくは、多機能分注ユニット11と、温度制御器60と、測定部54と、該多機能分注ユニット11、該多機能分注ユニット11内に設けられ、温度制御器60および前記測定部54について各種制御を行うCPU、ROM、RAM、各種の外部メモリ、LAN等の通信機能、およびROM等に格納されたプログラム等からなるCPU+プログラム70と、液晶ディスプレイ等の表示部や操作キー、タッチパネル等の操作部を有する操作パネル19とを有している。
該ノズル181は、内部に設けられた空洞181aと、分注チップ261、分離用チップ262、穿孔用チップ271が着脱可能に嵌合することによって装着可能で、前記発光等の光学的状態の変化に基づく光を受光する測定端521が設けられた前記先端部としての下部181cと、前記下部181cよりも大きな外径を持つ上部181bと、該上部181bの側面から側方に突出するように設けられ、前記空洞181aと連通し内部を気体が通過可能な通気管181dと、前記空洞181aの下端の開口部に嵌合するように設けられ、前記空洞181aを外部と連通させる3箇所の通気用の隙間181e,181f,181gが前記開口部との間に形成された栓181hとを有する。
該容器群201は、その長手方向がY軸方向に沿って設けられ、X軸方向に沿って並列した2つのカートリッジ容器241,211からなり、前記カートリッジ容器241は、液収容部群として反応容器や液収容部が1列状に配列され、前記カートリッジ容器211は、器具収容部群として前記多機能分注ユニット111のノズル181に装着して用いる種々の器具が1列状に配列されている。
該多機能分注ユニットを利用した核酸自動処理装置102は、内部を気体が通過可能な流路182aが貫通するノズル182と、気体の吸引および吐出を行う吸引吐出機構50として、管路502aを介して該ノズル182の前記流路182aと接続され、内部をピストンが摺動可能なシリンダ502bと、一端にピストン502dが設けられたピストンロッド502cと、前記ノズル182に装着された前記密閉蓋301を加熱するための加熱部511と、前記反応容器221内に密閉された前記増幅用溶液中に生ずる発光、呈色、変色、変光を含む光学的状態を測定可能な測定部542とを有する。
図13(a)は、該核酸自動処理装置103の外観を示すものであって、第1の実施の形態例に係る装置101と同様であるが、操作パネル192に表示される内容や操作は異なる。ここで、第1の実施の形態例と同一のものは、同一の符号で表しその説明を省略する。
ステップS17において、前記ノズル181を前記Y軸移動機構401yを用いてY軸に沿って移動させ収容部211Cの上方に位置させ、前記Z軸移動機構401zを用いて前記ノズル181を下降させて、該ノズル181の下部181cに前記分注チップ261の装着開口部に嵌合させて、該ノズル181に装着する。該分注チップ261は、前記X軸移動機構401xによって、X軸方向に移動させて前記カートリッジ容器241上に位置させた後、前記Y軸移動機構401yによりY軸方向に沿って移動させて前記液収容部231Hに位置させる。該液収容部231Hから前記核酸またはその断片を含有する溶液を前記吸引吐出機構501を用いて40μL吸引して、前記Y軸移動機構401yによって移送し、4つの前記反応容器221に順次10μLずつ分注する。その後、該分注チップ261は、前記X軸移動機構401xによって前記カートリッジ容器211上にまで移動させ、前記Y軸移動機構401yによってY軸方向に沿って該分注チップ261を移動させ、前記収容部211C上に位置させ、前記脱着機構531によって該分注チップ261を該収容部211C内に脱着する。
11,111,113,114 多機能分注ユニット
15,151 磁力部
16,161 ノズルヘッド
18,181,182,183 ノズル
19,191 操作パネル
20,(201),202,203 容器群(収容部連)
21(211) 器具収容部(群)(カートリッジ容器)
22,221 反応容器(群)
221a 細管部
221b 広口管部
23 試薬等収容部群
24(241) 液収容部(群)(カートリッジ容器)
26,261 分注チップ(群)
262 分離用チップ
29 密閉液
30,301 密閉蓋
32 分離抽出用溶液
33 増幅用溶液
40、401 移動機構
50,501 吸引吐出機構
52,521,522 測定端
53,531 脱着機構
54,541 測定部
55(551) 識別情報読取部(デジタルカメラ)
60,601 温度制御器
70 CPU+プログラム
72 核酸処理制御部
Claims (26)
- 気体の吸引および吐出を行う吸引吐出機構および該吸引吐出機構によって液体の吸引および吐出が可能な分注チップを着脱可能に装着する1または2以上のノズルが設けられたノズルヘッドと、
核酸増幅に用いる増幅用溶液を収容する1または2以上の液収容部および1または2以上の反応容器を少なくとも有する容器群と、
前記ノズルと前記容器群との間を相対的に移動可能とする移動機構と、
前記反応容器内を核酸増幅のための温度制御が可能な温度制御器と、
前記容器群の前記反応容器以外の所定収容部に収容され、前記ノズルを用いて前記反応容器にまで運搬可能であって該反応容器に収容した前記増幅用溶液を該反応容器内に密閉可能な密閉液および/または密閉蓋と、
前記反応容器への前記増幅用溶液の収容が完了すると、前記密閉液および/または密閉蓋が該増幅用溶液を前記反応容器内に密閉するように前記吸引吐出機構および前記移動機構、または前記移動機構を制御する密閉制御部と、を有する多機能分注ユニットを利用した核酸自動処理装置。 - 前記容器群には、検体、増幅対象である核酸またはその断片を捕獲可能な磁性粒子が懸濁した磁性粒子懸濁液、前記増幅対象の分離および抽出に用いる分離抽出用溶液を収容する2以上の液収容部をさらに有し、前記ノズルに装着した前記分注チップまたは前記容器群に設けられた液収容部の内部に磁場を及ぼしかつ除去することが可能で前記分注チップまたは前記液収容部の内壁に前記磁性粒子を吸着可能な磁力部をさらに有するとともに、前記吸引吐出機構、前記移動機構、および前記磁力部を制御して、前記検体から前記増幅対象の溶液を分離抽出して液収容部内に前記増幅用溶液の一部として収容する抽出制御部をさらに有する請求項1に記載の多機能分注ユニットを利用した核酸自動処理装置。
- 気体の吸引および吐出を行う吸引吐出機構および該吸引吐出機構によって液体の吸引および吐出が可能な分注チップを着脱可能に装着する1または2以上のノズルが設けられたノズルヘッドと、
核酸増幅に用いる増幅用溶液を収容する1または2以上の液収容部、1または2以上の反応容器、検体、増幅対象である核酸またはその断片を捕獲可能な磁性粒子が懸濁した磁性粒子懸濁液、前記増幅対象の分離および抽出に用いる分離抽出用溶液を収容する2以上の液収容部、および2以上の分注チップを装着可能に収容する2以上のチップ収容部を少なくとも有する容器群と、
前記分注チップを前記ノズルから脱着可能な脱着機構と、
前記ノズルと前記容器群との間を相対的に移動可能とする移動機構と、
前記反応容器内を核酸増幅のための温度制御が可能な温度制御器と、
前記ノズルに装着した前記分注チップまたは前記容器群に設けられた液収容部の内部に磁場を及ぼしかつ除去することが可能で前記分注チップまたは前記液収容部の内壁に前記磁性粒子を吸着可能な磁力部と、
前記容器群の前記反応容器以外の所定収容部に収容され、前記ノズルを用いて前記反応容器にまで運搬可能であって該反応容器に収容した前記増幅用溶液を該反応容器内に密閉可能な密閉液および/または密閉蓋と、
前記吸引吐出機構、前記移動機構、前記脱着機構および前記磁力部を制御して、前記分注チップを前記ノズルに装着して前記検体から前記増幅対象の溶液を分離抽出して液収容部内に前記増幅用溶液の一部として収容し前記分注チップを前記ノズルから脱着する抽出制御部と、
前記反応容器への前記増幅用溶液の収容が完了すると、前記密閉液および/または密閉蓋が該増幅用溶液を前記反応容器内に密閉するように前記吸引吐出機構および前記移動機構、または前記移動機構を制御する密閉制御部と、を有する多機能分注ユニットを利用した核酸自動処理装置。 - 前記密閉液および/または密閉蓋によって前記反応容器内に密閉された前記増幅用溶液中に生ずる発光、呈色、変色、変光を含む光学的状態を測定可能な測定部を有し、前記発光等に基づく光を受光する1または2以上の測定端を前記ノズルヘッドに設け、前記増幅対象を含む前記増幅用溶液を前記反応容器へ密閉した後または密閉する際に前記測定端を前記反応容器に接近させるように前記移動機構を制御して前記測定を可能とする測定制御部と、を有する請求項1乃至請求項3に記載の多機能分注ユニットを利用した核酸自動処理装置。
- 前記密閉液および/または密閉蓋は透光性を有し、前記測定制御部は、該反応容器内の前記増幅用溶液を密閉した前記密閉液および/または密閉蓋を通してその上側から前記光学的状態を測定可能とするように前記移動機構を制御する請求項4に記載の多機能分注ユニットを利用した核酸自動処理装置。
- 前記測定部は、前記吸引吐出機構と接続され前記ノズル内を通る流管の開口が設けられた前記ノズルの先端面を通して光の受光または照射可能な1または2以上の光ファイバが前記ノズル内を通るように設けられ、該先端面が前記測定端に相当する請求項4または請求項5のいずれかに記載の多機能分注ユニットを利用した核酸自動処理装置。
- 前記測定部の測定端は、前記ノズルの先端部と所定間隔を空けて離して前記ノズルと連動するように前記ノズルヘッドに設けられ、前記容器群の前記反応容器の開口部に上側から接近して前記密閉液および/または密閉蓋を介して受光または照射を可能とする請求項4または請求項5に記載の多機能分注ユニットを利用した核酸自動処理装置。
- 前記密閉制御部は、前記ノズルに前記分注チップを装着した後に、該分注チップで前記容器群の前記所定収容部から前記密閉液を所定量吸引して前記増幅用溶液が収容された前記反応容器に前記密閉液を吐出することで前記密閉液を運搬して前記増幅用溶液を反応容器内に密閉するように前記吸引吐出機構および前記移動機構を制御する請求項1乃至請求項57のいずれかに記載の多機能分注ユニットを利用した核酸自動処理装置。
- 温度制御可能な前記反応容器は、前記増幅用溶液が収容される細管部または薄管部と、該細管部または該薄管部と連通し該細管部または薄管部の上側に設けられ、前記細管部または薄管部の開口よりも広い開口を有し前記密閉液が収容される広口管部とを有するとともに、前記密閉制御部は、前記増幅用溶液量に基づいて、前記密閉液が前記広口管部にまで到達する量を前記反応容器に収容するように制御する請求項1乃至請求項8のいずれかに記載の多機能分注ユニットを利用した核酸自動処理装置。
- 前記密閉蓋は前記ノズルの先端部に嵌合して装着可能な嵌合部を有し、該密閉蓋および分注チップを前記ノズルから脱着する脱着機構を有するとともに、前記密閉制御部は分注チップをノズルに装着して前記増幅用溶液を前記反応容器に収容した後、分注チップをノズルから脱着して前記密閉蓋をノズルに装着して前記増幅用溶液を前記反応容器内に密閉するように前記吸引吐出機構、前記移動機構および前記脱着機構を制御する請求項1乃至請求項9のいずれかに記載の多機能分注ユニットを利用した核酸自動処理装置。
- 前記容器群には前記密閉液および密閉蓋を有し、該密閉蓋は前記ノズルの先端部に嵌合して装着可能な嵌合部を有し、該密閉蓋および分注チップを前記ノズルから脱着する脱着機構を有するとともに、前記密閉制御部は、前記増幅用溶液の前記反応容器内への収容が完了すると、前記密閉液を前記所定収容部に運搬した後、該分注チップをノズルから脱着して前記密閉蓋を前記ノズルに装着して該反応容器に運搬してその開口部を密閉するように前記移動機構および前記脱着機構を制御する請求項1乃至請求項10のいずれかに記載の多機能分注ユニットを利用した核酸自動処理装置。
- 前記密閉蓋が前記反応容器の開口部を閉塞した場合には、前記密閉制御部は、前記吸引吐出機構または前記移動機構を制御して該密閉蓋を押圧または振盪する請求項7乃至請求項11のいずれかに記載の多機能分注ユニットを利用した核酸自動処理装置。
- 前記密閉蓋を加熱する加熱部を、前記ノズルの先端部に設けるとともに、前記密閉制御部は前記密閉液が前記反応容器内に収容されていない場合には、該密閉蓋による前記反応容器の開口部の密閉の後に前記密閉蓋を加熱するように前記加熱部を制御する請求項1乃至請求項12のいずれかに記載の多機能分注ユニットを利用した核酸自動処理装置。
- 前記容器群は、液体を収容しまたは収容可能な1列状に形成された複数の収容部からなる液収容部群と、前記ノズルに装着して用いる器具を収容しまたは収容可能な1列状に形成される複数の収容部からなる器具収容部群とを並列に配列した1または2以上の収容部連からなり、前記液収容部群には、少なくとも前記温度制御器によって温度制御可能な1または2以上の前記反応容器、検体、核酸またはその断片の分離および抽出に用いる分離抽出用溶液、磁性粒子懸濁液および核酸若しくはその断片の増幅に用いる増幅用溶液を予め収容しまたは収容可能な試薬等収容部群を有し、前記器具収容部群には、少なくとも前記ノズルに装着可能な1または2以上の分注チップおよびプレパック用フィルムの穿孔を行う穿孔用チップを前記ノズルに装着可能な状態で収容しまたは収容可能であり、前記液収容部群には前記密閉液および/または前記器具収容部群には密閉蓋が収容された請求項1乃至請求項10のいずれかに記載の多機能分注ユニットを利用した核酸自動処理装置。
- 気体の吸引および吐出を行なう吸引吐出機構と、分注チップを着脱可能に装着する2以上のノズルを有するノズルヘッドと、1の前記ノズルが進入し他のノズルが進入しない各ノズルに対応した2以上の各専用領域内に配列され、核酸増幅に用いる増幅用溶液を収容する1または2以上の液収容部、核酸またはその断片を捕獲可能な磁性粒子が懸濁した磁性粒子懸濁液を収容する液収容部、検体を収容する液収容部、核酸またはその断片の分離および抽出に用いる分離抽出用溶液を収容する2以上の液収容部、および反応容器、前記ノズルを用いて前記反応容器にまで運搬可能であって前記反応容器に収容した前記増幅用溶液を前記反応容器内に密閉可能な密閉液および/または密閉蓋を少なくとも有する容器群と、前記各ノズルと前記各容器群との間を相対的に移動可能とするとともに各ノズルの移動を前記各専用領域内に制限する移動機構と、前記ノズルに装着された各分注チップの内壁に前記磁性粒子を吸着可能な磁力部と、前記反応容器内を核酸増幅のための温度制御が可能であって各容器群に設けられた温度制御器と、前記密閉液および/または密閉蓋によって前記反応容器内に密閉された前記増幅用溶液中に生ずる発光、呈色、変色または変光を含む光学的状態を測定可能であって、該発光等に基づく光を受光する各測定端を前記各専用領域に対応して前記ノズルヘッドに設けた測定部と、少なくとも前記吸引吐出機構、前記移動機構、前記温度制御器または前記磁力部を制御することによって、前記分注チップによる前記磁性粒子懸濁液および前記分離抽出用溶液を用いた検体からの核酸またはその断片の分離および抽出、前記分注チップによる抽出した該核酸またはその断片を含む前記増幅用溶液の混合、前記密閉液および/または密閉蓋による該増幅用溶液の前記反応容器への密閉、温度制御、および、前記測定端を前記密閉した前記反応容器に接近させて前記光学的状態の測定を各容器群に対して行なう核酸処理制御部とを有する多機能分注ユニットを利用した核酸自動処理装置。
- 容器群が有する核酸増幅に用いる増幅用溶液を収容する1または2以上の液収容部から、気体の吸引および吐出を行なう吸引吐出機構によって液体の吸引および吐出が可能な前記各ノズルに着脱可能に装着した分注チップ、前記気体の吸引吐出を行なう吸引吐出機構および前記ノズルと前記容器群との間を相対的に移動可能とする移動機構を用いて前記容器群に設けられた核酸増幅のための温度制御がされる反応容器に前記増幅用溶液を運搬し、前記吸引吐出機構および前記移動機構、または前記移動機構によって前記容器群の前記反応容器以外の所定収容部から密閉液および/または密閉蓋を前記反応容器内に前記ノズルを用いて運搬して前記増幅用溶液を前記反応容器内に密閉し、前記反応容器内を温度制御する多機能分注ユニットを利用した核酸自動処理方法。
- 前記容器群には、検体、増幅対象である核酸またはその断片を捕獲可能な磁性粒子が懸濁した磁性粒子懸濁液、前記増幅対象の分離および抽出に用いる分離抽出用溶液を収容する2以上の液収容部、および1または2以上の分注チップを収容するチップ収容部をさらに有し、前記分注チップを用いて前記検体と前記分離抽出用溶液としての該検体に含有するタンパク質を分解または溶解する溶解液とを混合して反応させ、該反応液と前記磁性粒子懸濁液とを混合して反応させて該磁性粒子に前記増幅対象を捕獲させ、前記ノズルヘッドに設けた磁力部を用いて、前記分注チップまたは液収容部内に磁場を及ぼすことで該分注チップまたは液収容部の内壁に前記磁性粒子を吸着させて磁性粒子を分離し、前記容器群に収容された他の分離抽出溶液としての解離液と前記磁性粒子とを接触させて、該磁性粒子から前記増幅対象を解離して、該増幅対象の溶液を前記増幅用溶液の一部として液収容部に収容する請求項16に記載の多機能分注ユニットを利用した核酸自動処理方法。
- 前記増幅用溶液を前記反応容器へ密閉した後または密閉する際に、測定端を前記移動機構を用いて前記反応容器に接近させて、該増幅用溶液中での光を受光して前記密閉液および/または密閉蓋で反応容器内に密閉した前記増幅用溶液中で生ずる発光、呈色、変色、変光を含む光学的状態を測定する請求項16に記載の多機能分注ユニットを利用した核酸自動処理方法。
- 前記測定工程は、前記ノズルヘッドに設けられた前記測定端を、前記移動機構を用いて、前記反応容器内に前記増幅用溶液を密閉した前記密閉液および/または密閉蓋の上側に位置させて、透光性のある密閉液および/または密閉蓋を通して前記増幅用溶液内を測定する請求項18に記載の多機能分注ユニットを利用した核酸自動処理方法。
- 前記測定工程は、前記ノズルの先端部と所定間隔を空けて離して該ノズルと連動するように前記ノズルヘッドに設けられた測定端を移動して前記反応容器の前記増幅用溶液を収容する部分の開口部の広さおよび形状に対応する光線束を前記密閉液および/または密閉蓋を介して入射しまたは照射する請求項18または請求項19に記載の多機能分注ユニットを利用した核酸自動処理方法。
- 前記密閉工程は、前記ノズルに前記分注チップを装着した後に、前記密閉液を、該分注チップ、前記吸引吐出機構および前記移動機構を用いて前記容器群の前記所定収容部から所定量運搬して前記反応容器内に吐出することで前記増幅用溶液を前記反応容器内に密閉する請求項17に記載の多機能分注ユニットを利用した核酸自動処理方法。
- 前記密閉は、分注チップをノズルに装着して前記増幅用溶液を反応容器に収容した後、該分注チップをノズルから脱着して、前記密閉蓋を、該密閉蓋の嵌合部で前記ノズルに装着して前記反応容器にまで運搬し、前記反応容器の開口部を閉塞することによって行う請求項17に記載の多機能分注ユニットを利用した核酸自動処理方法。
- 前記密閉は、前記増幅用溶液の前記反応容器内への収容が完了すると、前記密閉液を所定量前記反応容器に運搬し、さらに、該分注チップを前記ノズルから脱着し、前記密閉蓋を前記ノズルに装着して該反応容器に運搬してその開口部に嵌合する工程を有する請求項22に記載の多機能分注ユニットを利用した核酸自動処理方法。
- 前記密閉蓋を前記ノズルに装着して前記反応容器に運搬してその開口部を閉塞し、該密閉蓋を該ノズルに装着した状態で該ノズルまたは前記吸引吐出機構により押圧または振盪する工程を有する請求項18乃至請求項24に記載の多機能分注ユニットを利用した核酸自動処理方法。
- 前記密閉液が前記反応容器内に収容されていない場合には、前記反応容器の開口部を前記密閉蓋で閉塞した後、該反応容器の温度制御の際に、前記ノズルの先端部を加熱することによって前記密閉蓋を加熱する請求項16乃至請求項23のいずれかに記載の多機能分注ユニットを利用した核酸自動処理方法。
- ノズルヘッドに設けた2以上のノズルに対し、1の前記ノズルが進入し他のノズルが進入しない各専用領域内に設けられた容器群が有する検体、磁性粒子懸濁液、核酸またはその断片の分離および抽出に用いる分離抽出用溶液を収容する液収容部群から、前記ノズルに着脱可能に装着した分注チップ、気体の吸引吐出を行なう吸引吐出機構および前記ノズルと前記容器群との間を相対的に移動可能とするとともに各ノズルの移動を前記専用領域内に制限する移動機構を用いて、前記検体、および前記分離抽出用溶液としてのタンパク質を分解または溶解する溶解液を反応容器にまで運搬し混合して反応させ、前記磁性粒子懸濁液を混合して反応させ、該磁性粒子に検体から得られた増幅対象の核酸またはその断片を捕獲させ、前記ノズルヘッドに設けた磁力部によって前記分注チップまたは液収容部内に磁場を及ぼすことでその内壁に前記磁性粒子を吸着させて分離し、分離した磁性粒子と解離液とを接触させることで前記核酸またはその断片を解離し、解離した核酸またはその断片を前記増幅用溶液の一部として前記吸引吐出機構および前記移動機構によって温度制御可能な反応容器に収容して混合し、
該増幅用溶液を、前記吸引吐出機構および前記移動機構、または前記移動機構によって前記容器群に収容された密閉液および/または密閉蓋で前記反応容器に密閉し、
密閉された前記増幅用溶液について前記温度制御器を用いて温度制御し、
前記密閉液および/または密閉蓋によって前記反応容器内に密閉された前記増幅用溶液中に生ずる発光、呈色、変色または変光を含む光学的状態を測定部の測定端を、前記密閉した前記反応容器に接近させて測定する多機能分注ユニットを利用した核酸自動処理方法。
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2012538730A JP5830024B2 (ja) | 2010-10-15 | 2011-10-14 | 多機能分注ユニットを利用した核酸自動処理装置およびその方法 |
CN201180049826.1A CN103237880B (zh) | 2010-10-15 | 2011-10-14 | 利用多功能分注单元的核酸自动处理装置及其方法 |
EP11832627.1A EP2628786B1 (en) | 2010-10-15 | 2011-10-14 | Automated nucleic acid processor and automated nucleic acid processing method using multi function dispensing unit |
KR1020137012018A KR101852646B1 (ko) | 2010-10-15 | 2011-10-14 | 다기능 분주 유닛을 이용한 핵산 자동 처리장치 및 그 방법 |
US13/879,069 US9057101B2 (en) | 2010-10-15 | 2011-10-14 | Automated nucleic acid processor and automated nucleic acid processing method using multi function dispensing unit |
US14/707,900 US9797008B2 (en) | 2010-10-15 | 2015-05-08 | Automated nucleic acid processor and automated nucleic acid processing method using multi function dispensing unit |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2010233057 | 2010-10-15 | ||
JP2010-233057 | 2010-10-15 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US13/879,069 A-371-Of-International US9057101B2 (en) | 2010-10-15 | 2011-10-14 | Automated nucleic acid processor and automated nucleic acid processing method using multi function dispensing unit |
US14/707,900 Division US9797008B2 (en) | 2010-10-15 | 2015-05-08 | Automated nucleic acid processor and automated nucleic acid processing method using multi function dispensing unit |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2012050198A1 true WO2012050198A1 (ja) | 2012-04-19 |
Family
ID=45938418
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2011/073697 WO2012050198A1 (ja) | 2010-10-15 | 2011-10-14 | 多機能分注ユニットを利用した核酸自動処理装置およびその方法 |
Country Status (6)
Country | Link |
---|---|
US (2) | US9057101B2 (ja) |
EP (1) | EP2628786B1 (ja) |
JP (1) | JP5830024B2 (ja) |
KR (1) | KR101852646B1 (ja) |
CN (1) | CN103237880B (ja) |
WO (1) | WO2012050198A1 (ja) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103013814A (zh) * | 2012-12-20 | 2013-04-03 | 嘉兴凯实生物科技有限公司 | 一种核酸纯化装置 |
WO2014189085A1 (ja) * | 2013-05-21 | 2014-11-27 | ユニバーサル・バイオ・リサーチ株式会社 | シーケンサ前処理装置およびその方法 |
JP2017153405A (ja) * | 2016-02-29 | 2017-09-07 | シスメックス株式会社 | 検体前処理装置、検体前処理カートリッジおよび検体前処理方法 |
WO2018207875A1 (ja) * | 2017-05-12 | 2018-11-15 | ユニバーサル・バイオ・リサーチ株式会社 | 核酸検出用カートリッジ |
JPWO2018181481A1 (ja) * | 2017-03-28 | 2020-02-06 | ユニバーサル・バイオ・リサーチ株式会社 | 測光分注ノズルユニット、測光分注装置、および測光分注処理方法 |
JP2020042051A (ja) * | 2016-02-29 | 2020-03-19 | シスメックス株式会社 | 検体前処理カートリッジ |
WO2023032870A1 (ja) * | 2021-09-01 | 2023-03-09 | ユニバーサル・バイオ・リサーチ株式会社 | Pcr装置 |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2591198B (en) | 2014-04-04 | 2021-10-27 | It Is Int Ltd | Biochemical reaction system |
US9827567B2 (en) | 2014-04-22 | 2017-11-28 | Nanosphere, Inc. | Diagnostic cartridges having flexible seals |
CN105940094B (zh) * | 2014-06-17 | 2018-02-23 | 深圳迈瑞生物医疗电子股份有限公司 | 核酸提取装置及其工作方法 |
US20180003635A1 (en) * | 2015-01-20 | 2018-01-04 | Hyris Limited | Detector for measuring fluorescence in a liquid sample |
FR3034431B1 (fr) * | 2015-04-01 | 2019-06-07 | Ad Nucleis | Installation et procede de traitement automatise des donnees d’un robot d’extraction des acides nucleiques et d’amplification de genes par le procede pcr temps reel |
CN109070084A (zh) | 2016-03-18 | 2018-12-21 | 安德鲁联合有限公司 | 液体处理器的尖端中的珠子操作方法及装置 |
US20200187863A1 (en) * | 2017-06-23 | 2020-06-18 | Voyant Diagnostics, Inc. | Sterile Urine Collection Mechanism for Medical Diagnostic Systems |
CN107964505A (zh) * | 2017-12-28 | 2018-04-27 | 广州市宝创生物技术有限公司 | 多功能核酸检测装置 |
GB2589159B (en) | 2017-12-29 | 2023-04-05 | Clear Labs Inc | Nucleic acid sequencing apparatus |
US11459604B2 (en) | 2018-07-12 | 2022-10-04 | Luminex Corporation | Systems and methods for performing variable sample preparation and analysis processes |
JP7139931B2 (ja) * | 2018-12-14 | 2022-09-21 | 株式会社島津製作所 | 磁性体粒子操作用容器及び磁性体粒子操作用装置 |
CN110257225A (zh) * | 2019-08-06 | 2019-09-20 | 中国人民解放军军事科学院军事医学研究院 | 隔离装置及核酸提取仪 |
JP1678417S (ja) * | 2020-05-29 | 2021-02-01 | ||
CN113908900A (zh) * | 2021-09-22 | 2022-01-11 | 西安洛科电子科技股份有限公司 | 一种便于安装的无缆式智能分注仪及其安装方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07287019A (ja) * | 1994-04-09 | 1995-10-31 | Boehringer Mannheim Gmbh | 反応連鎖の無汚染処理装置および方法 |
JP2622327B2 (ja) | 1985-03-28 | 1997-06-18 | エフ.ホフマン−ラ ロシュ アクチェンゲゼルシャフト | 核酸配列の増幅手段 |
US5958349A (en) | 1997-02-28 | 1999-09-28 | Cepheid | Reaction vessel for heat-exchanging chemical processes |
JP2000511435A (ja) | 1996-06-04 | 2000-09-05 | ユニバーシティ オブ ユタ リサーチ ファウンデーション | 生物学的プロセスを実行し且つモニタリングするためのシステムと方法 |
JP2002010777A (ja) | 2000-06-30 | 2002-01-15 | Precision System Science Co Ltd | 反応容器、反応装置および反応液の温度制御方法 |
JP2005095134A (ja) * | 2003-08-20 | 2005-04-14 | Sysmex Corp | 核酸検出装置 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69634794T2 (de) * | 1995-03-20 | 2006-04-27 | PRECISION SYSTEM SCIENCE Co., Ltd., Matsudo | Verfahren und gerät für die flüssigkeitsbehandlung mittels eines verteilers |
CN1965073A (zh) * | 2004-06-02 | 2007-05-16 | 爱科来株式会社 | 核酸扩增用容器、核酸调制试剂盒和核酸分析装置 |
JP4820756B2 (ja) * | 2004-08-05 | 2011-11-24 | ユニバーサル・バイオ・リサーチ株式会社 | 反応容器、反応容器液導入装置、液導入反応測定装置、および液導入装置 |
JP4792277B2 (ja) * | 2005-11-24 | 2011-10-12 | 株式会社島津製作所 | 反応容器及び反応容器処理装置 |
US7998708B2 (en) * | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
US8418929B2 (en) * | 2007-04-06 | 2013-04-16 | Universal Bio Research Co., Ltd. | Temperature controlling apparatus and temperature controlling method |
TWI391492B (zh) | 2007-11-20 | 2013-04-01 | Quanta Comp Inc | 自動化遺傳物質處理系統及方法 |
CN101838609B (zh) * | 2010-03-15 | 2012-06-20 | 上海浩源生物科技有限公司 | 加样装置及其应用 |
-
2011
- 2011-10-14 KR KR1020137012018A patent/KR101852646B1/ko active IP Right Grant
- 2011-10-14 JP JP2012538730A patent/JP5830024B2/ja not_active Expired - Fee Related
- 2011-10-14 US US13/879,069 patent/US9057101B2/en active Active
- 2011-10-14 CN CN201180049826.1A patent/CN103237880B/zh active Active
- 2011-10-14 WO PCT/JP2011/073697 patent/WO2012050198A1/ja active Application Filing
- 2011-10-14 EP EP11832627.1A patent/EP2628786B1/en active Active
-
2015
- 2015-05-08 US US14/707,900 patent/US9797008B2/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2622327B2 (ja) | 1985-03-28 | 1997-06-18 | エフ.ホフマン−ラ ロシュ アクチェンゲゼルシャフト | 核酸配列の増幅手段 |
JPH07287019A (ja) * | 1994-04-09 | 1995-10-31 | Boehringer Mannheim Gmbh | 反応連鎖の無汚染処理装置および方法 |
JP2000511435A (ja) | 1996-06-04 | 2000-09-05 | ユニバーシティ オブ ユタ リサーチ ファウンデーション | 生物学的プロセスを実行し且つモニタリングするためのシステムと方法 |
US5958349A (en) | 1997-02-28 | 1999-09-28 | Cepheid | Reaction vessel for heat-exchanging chemical processes |
JP2002010777A (ja) | 2000-06-30 | 2002-01-15 | Precision System Science Co Ltd | 反応容器、反応装置および反応液の温度制御方法 |
JP2005095134A (ja) * | 2003-08-20 | 2005-04-14 | Sysmex Corp | 核酸検出装置 |
Non-Patent Citations (2)
Title |
---|
HIDEJI TAJIMA: "System for Automated DNA Extraction Using Magnetic Particles", JOURNAL OF MAGNETICS SOCIETY OF JAPAN, vol. 22, no. 5, 1998, pages 1010 - 1015, XP002926135 * |
See also references of EP2628786A4 |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103013814A (zh) * | 2012-12-20 | 2013-04-03 | 嘉兴凯实生物科技有限公司 | 一种核酸纯化装置 |
US10626440B2 (en) | 2013-05-21 | 2020-04-21 | Universal Bio Research Co., Ltd. | Sequencer pretreatment device and method thereof |
WO2014189085A1 (ja) * | 2013-05-21 | 2014-11-27 | ユニバーサル・バイオ・リサーチ株式会社 | シーケンサ前処理装置およびその方法 |
JPWO2014189085A1 (ja) * | 2013-05-21 | 2017-02-23 | ユニバーサル・バイオ・リサーチ株式会社 | シーケンサ前処理装置およびその方法 |
US11060081B2 (en) | 2016-02-29 | 2021-07-13 | Sysmex Corporation | Specimen pretreatment apparatus, specimen pretreatment cartridge, and specimen pretreatment method |
JP2020042051A (ja) * | 2016-02-29 | 2020-03-19 | シスメックス株式会社 | 検体前処理カートリッジ |
JP2017153405A (ja) * | 2016-02-29 | 2017-09-07 | シスメックス株式会社 | 検体前処理装置、検体前処理カートリッジおよび検体前処理方法 |
JPWO2018181481A1 (ja) * | 2017-03-28 | 2020-02-06 | ユニバーサル・バイオ・リサーチ株式会社 | 測光分注ノズルユニット、測光分注装置、および測光分注処理方法 |
JP7201241B2 (ja) | 2017-03-28 | 2023-01-10 | ユニバーサル・バイオ・リサーチ株式会社 | 測光分注ノズルユニット、測光分注装置、および測光分注処理方法 |
JPWO2018207875A1 (ja) * | 2017-05-12 | 2020-03-12 | ユニバーサル・バイオ・リサーチ株式会社 | 核酸検出用カートリッジ |
WO2018207875A1 (ja) * | 2017-05-12 | 2018-11-15 | ユニバーサル・バイオ・リサーチ株式会社 | 核酸検出用カートリッジ |
JP7165651B2 (ja) | 2017-05-12 | 2022-11-04 | ユニバーサル・バイオ・リサーチ株式会社 | 核酸検出用カートリッジ |
WO2023032870A1 (ja) * | 2021-09-01 | 2023-03-09 | ユニバーサル・バイオ・リサーチ株式会社 | Pcr装置 |
Also Published As
Publication number | Publication date |
---|---|
US9797008B2 (en) | 2017-10-24 |
KR20140006791A (ko) | 2014-01-16 |
US20150315630A1 (en) | 2015-11-05 |
KR101852646B1 (ko) | 2018-04-26 |
CN103237880A (zh) | 2013-08-07 |
JP5830024B2 (ja) | 2015-12-09 |
CN103237880B (zh) | 2016-08-03 |
US9057101B2 (en) | 2015-06-16 |
US20130288259A1 (en) | 2013-10-31 |
EP2628786A4 (en) | 2015-09-30 |
EP2628786B1 (en) | 2019-01-02 |
EP2628786A1 (en) | 2013-08-21 |
JPWO2012050198A1 (ja) | 2014-02-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5830024B2 (ja) | 多機能分注ユニットを利用した核酸自動処理装置およびその方法 | |
JP6134018B2 (ja) | 自動反応・光測定装置およびその方法 | |
JP6294823B2 (ja) | 反応容器用光測定装置およびその方法 | |
JP5991967B2 (ja) | 反応容器用光測定装置およびその方法 | |
JP5877192B2 (ja) | 反応容器およびその製造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 11832627 Country of ref document: EP Kind code of ref document: A1 |
|
DPE1 | Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101) | ||
ENP | Entry into the national phase |
Ref document number: 2012538730 Country of ref document: JP Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2011832627 Country of ref document: EP |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
ENP | Entry into the national phase |
Ref document number: 20137012018 Country of ref document: KR Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 13879069 Country of ref document: US |