WO2012033334A3 - 부탄올 생성능이 개선된 재조합 미생물 및 이를 이용한 부탄올의 제조방법 - Google Patents

부탄올 생성능이 개선된 재조합 미생물 및 이를 이용한 부탄올의 제조방법 Download PDF

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WO2012033334A3
WO2012033334A3 PCT/KR2011/006607 KR2011006607W WO2012033334A3 WO 2012033334 A3 WO2012033334 A3 WO 2012033334A3 KR 2011006607 W KR2011006607 W KR 2011006607W WO 2012033334 A3 WO2012033334 A3 WO 2012033334A3
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butanol
recombinant microorganism
coa
enzyme
produce
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WO2012033334A2 (ko
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이상엽
최용준
박진환
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한국과학기술원
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Publication of WO2012033334A3 publication Critical patent/WO2012033334A3/ko

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    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/07—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with an iron-sulfur protein as acceptor (1.2.7)
    • C12Y102/07007—3-Methyl-2-oxobutanoate dehydrogenase (ferredoxin) (1.2.7.7)
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
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    • C12R2001/15—Corynebacterium
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    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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Abstract

본 발명은 부탄올 생성능이 개선된 재조합 미생물 및 이를 이용한 부탄올의 제조방법에 관한 것으로서, 더욱 상세하게는 L-발린의 전구체인 2-ketoisovalerate를 중간체(intermediate)로 하여 부탄올을 생합성하는 재조합 미생물 및 이를 이용한 부탄올의 제조방법에 관한 것이다. 본 발명에 따른 재조합 미생물은 L-발린의 전구체인 2-ketoisovalerate의 생성능을 가지는 미생물에, 2-ketoisovalerate를 isobutyryl-CoA로 전환시키는 효소인 VorABC 효소를 코딩하는 유전자, isobutyryl-CoA를 butyryl-CoA로 전환시키는 효소를 코딩하는 유전자, butyryl-CoA를 butyraldehyde로 전환시키는 효소를 코딩하는 유전자 및 butyraldehyde를 butanol로 전환시키는 효소를 코딩하는 유전자가 도입되어 있는 것을 특징으로 한다. 본 발명에 따른 재조합 미생물은 기존의 Clostridium acetobutylicum과달리성장이용이하고, 추가 대사흐름 조작으로 인한 균주개량의 가능성이 크므로 부탄올의 산업적 생성 미생물로 추가 대사흐름 조작으로 인한 균주개량의 가능성이 크므로 부탄올의 산업적 생성 미생물로 유용하며, 2-ketoisovalerate를 isobutyryl-CoA로 전환시키는 과정을 단순화시킴으로써 부탄올 생성능이 개선된 재조합 미생물을 제공하는 효과가 있다.
PCT/KR2011/006607 2010-09-07 2011-09-07 부탄올 생성능이 개선된 재조합 미생물 및 이를 이용한 부탄올의 제조방법 WO2012033334A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100087550A KR101697368B1 (ko) 2010-09-07 2010-09-07 부탄올 생성능이 개선된 재조합 미생물 및 이를 이용한 부탄올의 제조방법
KR10-2010-0087550 2010-09-07

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WO2012033334A2 WO2012033334A2 (ko) 2012-03-15
WO2012033334A3 true WO2012033334A3 (ko) 2012-08-23

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Publication number Priority date Publication date Assignee Title
US11692207B2 (en) 2016-05-05 2023-07-04 Newpek S.A. De C.V. Enzymatic methods for butanol production

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100832740B1 (ko) * 2007-01-17 2008-05-27 한국과학기술원 분지쇄 아미노산 생성능이 개선된 변이 미생물 및 이를이용한 분지쇄 아미노산의 제조방법
KR20080060220A (ko) * 2006-12-15 2008-07-01 바이오퓨얼켐 주식회사 부탄올 생성능이 개선된 재조합 변이 미생물 및 이를이용한 부탄올의 제조방법
KR20110002130A (ko) * 2009-07-01 2011-01-07 한국과학기술원 부탄올 생성능을 가지는 재조합 미생물 및 이를 이용한 부탄올의 제조방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080060220A (ko) * 2006-12-15 2008-07-01 바이오퓨얼켐 주식회사 부탄올 생성능이 개선된 재조합 변이 미생물 및 이를이용한 부탄올의 제조방법
KR20080070807A (ko) * 2006-12-15 2008-07-31 바이오퓨얼켐 주식회사 Butyryl-CoA를 중간체로 하여 부탄올을 생합성하는 능력을가지는 박테리아를 이용하여 부탄올을 제조하는 방법
KR100832740B1 (ko) * 2007-01-17 2008-05-27 한국과학기술원 분지쇄 아미노산 생성능이 개선된 변이 미생물 및 이를이용한 분지쇄 아미노산의 제조방법
KR20110002130A (ko) * 2009-07-01 2011-01-07 한국과학기술원 부탄올 생성능을 가지는 재조합 미생물 및 이를 이용한 부탄올의 제조방법

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHO, A. ET AL.: "Prediction of novel synthetic pathways for the production of desired chemicals.", BMC SYSTEMS BIOLOGY., vol. 4, 28 March 2010 (2010-03-28), pages 35 - 50 *

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KR101697368B1 (ko) 2017-01-17
KR20120025262A (ko) 2012-03-15

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