WO2011154959A1 - Méthode avancée d'élaboration d'une préparation bactérienne à base de pseudomonas fluorescens pour le contrôle de différentes maladies des plantes cultivées - Google Patents

Méthode avancée d'élaboration d'une préparation bactérienne à base de pseudomonas fluorescens pour le contrôle de différentes maladies des plantes cultivées Download PDF

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Publication number
WO2011154959A1
WO2011154959A1 PCT/IN2010/000387 IN2010000387W WO2011154959A1 WO 2011154959 A1 WO2011154959 A1 WO 2011154959A1 IN 2010000387 W IN2010000387 W IN 2010000387W WO 2011154959 A1 WO2011154959 A1 WO 2011154959A1
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WIPO (PCT)
Prior art keywords
rot
pac
culture
advanced bio
fungicide according
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PCT/IN2010/000387
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English (en)
Inventor
S. Patel.Chetan
Original Assignee
Patel, Babubhai C.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Patel, Babubhai C. filed Critical Patel, Babubhai C.
Priority to PCT/IN2010/000387 priority Critical patent/WO2011154959A1/fr
Publication of WO2011154959A1 publication Critical patent/WO2011154959A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/27—Pseudomonas

Definitions

  • the present invention relates generally to a material and advanced method of making a bacterial formation using Pseudomonas fluorescens for controlling various diseases of crop plant.
  • the invention relates to a process for the production of organic and combination formulation of bio-pesticide containing Trichoderma harzianum and Pseudomonas fluorescens comprising preparation of mother culture, liquid fermentation as well as solid fermentation of T. harzianum, preparation of mother culture, liquid fermentation as well as solid fermentation of P. fluorescens separately, followed by mixing both the bio-pesticides in the proportion of 1-2: 1-2: preferably 1 : 1 to get the final combination, formulation..
  • the invention relates to a process for the production of organic formulation of bio pesticide containing Pseudomonas Fluorescens comprising preparation of Mother culture of P. fluorescens and inoculating in King"s B broth, at 30 + 1° C for 24-36 hours followed by
  • the invention relates to a novel process for commercial production of biopesticides has been invented.
  • the process involves two steps. In the first step, mass culture or stock culture of biocontrol fungi and bacteria is prepared on saw dust, soil and molasses mixture.
  • the second step involves immobilization of the bioagents on a fly ash based carrier.
  • three biopesticides based on Trichoderma harzianum, Pochonia chlamydosporia and Pseudomonas fluorescens have been developed.
  • the three biopesticides with their names and the diseases they control are as follows. 1.
  • Biowilt-X is a Biofungicide of Trichoderma harzianum to control fusarial wilt disease caused by fusarium spp. 2.
  • Bionem-X is a Bionematicide of Pochonia chlamydosporia to control root-knot disease caused by Meloidogyne spp. 3.
  • Biocomp-X is a Biofunginemacide of Pseudomonas fluorescens to control wilt disease complex caused by Meloidogyne and Fusarium spp. jointly. '
  • the invention relates to a process for mass multiplication of Pseudomonas fluorescens on cow dung comprising the steps of air drying of cow dung by spreading to form layer of 2.5 thickness under open shade for 5 to 7 days, maintaining the moisture content to 40 % by weight of air dried cow dung by adding water, maintaining the pH of cow dung between 7 to 7.5, autoclaving the cow dung of step b) at 15 lbs for 30 minutes, 2 ml to 5 ml suspension of Pseudomonas fluorescens PBAP-27 talc-based formulation (@ lg/ 100 ml; approx.
  • the invention relates to to a low cost method of producing peptides, including antimicrobial peptides (AMPs), by using microbes.
  • AMPs antimicrobial peptides
  • the subject methods enable greatly improved yields of the peptide/AMP as compared to those heretofore known in the art.
  • the subject methods also surprisingly enable the use of Pseudomonas fluorescens to produce AMPs and other peptides.
  • the subject invention provides for the production of peptides/AMPs in concatemeric precursors.
  • the subject invention also provides novel methods of assembling monomers into multimers, and of cleaving the multimers to yield active monomers.
  • the subject invention also relates to the use of these multimers fused .to carrier peptides to produce fusion proteins.
  • both the multimers and the fusion proteins (multimers with the carrier polypeptides) lack charge balancing. It has been surprisingly determined that, it is not necessary to offset the positive charges of multiple copies of AMPs in multimeric constructs. Thus, the subject invention enables the use of a wider range of multimers and carrier peptides.
  • the invention relates to purified bacterial strains that are effective for the inhibition of plant pathogens, including the fungi Rhizoctonia solani and Pythium ultimum that have been isolated. These strains are useful as biocontrol agents, and can be used to produce antifungal metabolites, such as antibiotic compounds, active against the plant pathogenic fungi Rhizoctonia solani and Pythium ultimum. Both the purified bacterial strains and the antibiotic compounds can be used as active agents for biocontrol compositions.
  • Soil borrie disease have until recently, received less attention than plant diseases affecting the shoot and foliage. However, this is not a reflection of their economic importance, but rater of the difficulties in investigating and detecting pathogens below soil level. Many soil born diseases are stress related and it is in the tropics where crop growth is particularly limited by environmental stress, predisposing crops to infection by soil borne pathogen.
  • This invention relates to a method for utilizing bacterial based product containing Pseudomonas fluoresence to control various soil borne diseases like root rot, set rot, wilt, collar rot, red rot, rhizome rot, damping off, stem rot, club rot and alike and as well as managing disease like blight, blast, all leaf spot, anthracnose and alike.
  • the present invention has been made with the foregoing background in mind and its object resides in providing a material and method of rationally producing a high quality of method for utilizing bacterial based product containing Pseudomonas fluoresence to control various soil borne diseases at the highest economical level using raw material which can be easily procured at the most inexpensive cost.
  • the inventor has invented a material and method for improving crop yields, utilizing bacterial based product containing Pseudomonas fluoresence to control various soil borne diseases like root rot, set rot, wilt, collar rot, red rot, rhizome rot, damping off, stem rot, club rot and alike and as well as managing disease like blight, blast, all leaf spot, anthracnose and alike.
  • the subject invention concerns an advanced combination of bacterial based product containing Pseudomonas fluoresence with enzymes, fats and growth promoting molecules to control soil borne diseases and manage disease like blight, blast, all leaf spot, anthracnose and alike.
  • Step-3 Growth culture (In 1000 ml flask)
  • step-2 After sufficient growth in 250 ml flask (step-2), 50 ml culture from this grown culture, is inoculated aseptically in 500 ml PAC-07 broth media in a 1000 ml capacity flask for further growth at 27 ⁇ 1 °C for at least 3 to 4 days on shaker with 150 RPM, such two flasks is prepared.
  • Composition for one liter of PAC-07 broth media contains 10 gm Beef extract, 10 gm Peptone, 5 gm NaCl and 1000 ml distilled water
  • Step-4 Seed Fermentor (In small fermentor of 10 lit capacities)
  • One (1) lit grown culture in step-3 is inoculated aseptically in small fermenter having 10 litter PAC-07 broth media, and allow for further growth at 27 ⁇ 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM.
  • Composition for one liter of PAC-07 broth media contains 10 gm Beef extract, 10 gm Peptone, 5 gm NaCl and 1000 ml distilled water)
  • Step-5 Production Fermentor (In Big fermentor of 200 lit capacities)
  • Ten (10) lit culture grown in step-4 is inoculated aseptically in Big fermenter having 200 litter PAC-07 media (Composition for one liter of PAC-07 broth media contains 10 gm Beef extract, 10 gm Peptone, 5 gm NaCl and 1000 ml distilled water), and allowed for further growth at 27 ⁇ 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM Step-6 Bacterial Separation
  • Recovered bacteria form centrifuge is mix in a set of ration with suitable carrier composed of zero TDS water etc and than homogenized it for equivalently of formulation.
  • Material prepared as above is then packed in 100 ml, 250 ml and 500 ml packing by using bottle or pouch packing machine.
  • Step-09 Distrubution to farmer,retailer,,distributor etc
  • the final material prepared has to be used in following form:

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention porte sur des matériaux et méthodes d'élaboration d'un fongicide bio avancé à partir d'une combinaison novatrice de bactéries Pseudomonas fluorescens et d'enzymes, de graisses et de molécules de promotion de croissance, pour lutter contre des maladies telluriques du type: pourridié, pourriture du semenceau, flétrissures, pourriture du collet, pourriture rouge, pourriture du rhizome, fonte des semis, pourriture de la tige, hernie etc., et pour gérer des maladies du type: brûlure, coulure, tache entière des feuilles, anthracnose etc.
PCT/IN2010/000387 2010-06-09 2010-06-09 Méthode avancée d'élaboration d'une préparation bactérienne à base de pseudomonas fluorescens pour le contrôle de différentes maladies des plantes cultivées WO2011154959A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/IN2010/000387 WO2011154959A1 (fr) 2010-06-09 2010-06-09 Méthode avancée d'élaboration d'une préparation bactérienne à base de pseudomonas fluorescens pour le contrôle de différentes maladies des plantes cultivées

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IN2010/000387 WO2011154959A1 (fr) 2010-06-09 2010-06-09 Méthode avancée d'élaboration d'une préparation bactérienne à base de pseudomonas fluorescens pour le contrôle de différentes maladies des plantes cultivées

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WO2011154959A1 true WO2011154959A1 (fr) 2011-12-15

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3844893A (en) * 1973-02-12 1974-10-29 Phillips Petroleum Co Microbial synthesis
US3911112A (en) * 1973-07-10 1975-10-07 Ayers Mckenna And Harrison Ltd Antibiotic substances and preparation
US5260302A (en) * 1989-10-17 1993-11-09 Imperial Chemical Industries Plc Antifungal microorganism
US5711945A (en) * 1995-05-11 1998-01-27 Regents Of The University Of Minnesota Pitch degradation with Pseudomonas fluorescens
US6280719B1 (en) * 1997-02-05 2001-08-28 Hyung-Won Suh Antifungal biocontrol agents, a process for preparing and treating the same
US6495362B1 (en) * 1996-09-03 2002-12-17 Council Of Scientific & Industrial Research Biologically pure culture of bacteria which suppresses diseases caused by pathogens in chickpea crops and a culture of bacteria comprising a strain of Pseudomonas fluorescens
WO2007054691A1 (fr) * 2005-11-08 2007-05-18 The University Of Surrey Materiaux de bioremediation
WO2008139488A1 (fr) * 2007-05-10 2008-11-20 Department Of Biotechnology Procédé de production d'une composition organique de biopesticide à base de pseudomonas fluorescens
US20080286853A1 (en) * 2006-02-16 2008-11-20 Department Of Biotechnology (Dbt) Process for Producing a Bio-Pesticide Composition Containing Trichoderma Harzianum and Pseudomonas Fluorescens

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3844893A (en) * 1973-02-12 1974-10-29 Phillips Petroleum Co Microbial synthesis
US3911112A (en) * 1973-07-10 1975-10-07 Ayers Mckenna And Harrison Ltd Antibiotic substances and preparation
US5260302A (en) * 1989-10-17 1993-11-09 Imperial Chemical Industries Plc Antifungal microorganism
US5711945A (en) * 1995-05-11 1998-01-27 Regents Of The University Of Minnesota Pitch degradation with Pseudomonas fluorescens
US6495362B1 (en) * 1996-09-03 2002-12-17 Council Of Scientific & Industrial Research Biologically pure culture of bacteria which suppresses diseases caused by pathogens in chickpea crops and a culture of bacteria comprising a strain of Pseudomonas fluorescens
US6280719B1 (en) * 1997-02-05 2001-08-28 Hyung-Won Suh Antifungal biocontrol agents, a process for preparing and treating the same
WO2007054691A1 (fr) * 2005-11-08 2007-05-18 The University Of Surrey Materiaux de bioremediation
US20080286853A1 (en) * 2006-02-16 2008-11-20 Department Of Biotechnology (Dbt) Process for Producing a Bio-Pesticide Composition Containing Trichoderma Harzianum and Pseudomonas Fluorescens
WO2008139488A1 (fr) * 2007-05-10 2008-11-20 Department Of Biotechnology Procédé de production d'une composition organique de biopesticide à base de pseudomonas fluorescens

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NAUTIYAL: "A Method for Selection and Characterization of Rhizosphere-Competent Bacteria of Chickpea.", CURRENT MICROBIOLOGY, vol. 34, no. 1, January 1997 (1997-01-01), pages 12 - 17 *
VIDHYASEKARAN ET AL.: "Development of Formulations of Pseudomonas fluorescens for Control of Chickpea Wilt", PLANT DISEASE, vol. 79, no. 8, August 1995 (1995-08-01), pages 782 - 786 *

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