WO2011150726A1 - 一种干细胞过滤筛选富集并快速复合装置 - Google Patents

一种干细胞过滤筛选富集并快速复合装置 Download PDF

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Publication number
WO2011150726A1
WO2011150726A1 PCT/CN2011/073262 CN2011073262W WO2011150726A1 WO 2011150726 A1 WO2011150726 A1 WO 2011150726A1 CN 2011073262 W CN2011073262 W CN 2011073262W WO 2011150726 A1 WO2011150726 A1 WO 2011150726A1
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WIPO (PCT)
Prior art keywords
stem cell
filter
enrichment
screening enrichment
composite device
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PCT/CN2011/073262
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English (en)
French (fr)
Inventor
戴尅戎
干耀恺
梁栋科
李涛
林森
Original Assignee
上海康德莱企业发展集团医疗器械有限公司
上海交通大学医学院附属第九人民医院
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Application filed by 上海康德莱企业发展集团医疗器械有限公司, 上海交通大学医学院附属第九人民医院 filed Critical 上海康德莱企业发展集团医疗器械有限公司
Publication of WO2011150726A1 publication Critical patent/WO2011150726A1/zh

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/38—Removing constituents from donor blood and storing or returning remainder to body, e.g. for transfusion
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/02—Blood transfusion apparatus
    • A61M1/0281—Apparatus for treatment of blood or blood constituents prior to transfusion, e.g. washing, filtering or thawing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2202/00—Special media to be introduced, removed or treated
    • A61M2202/04—Liquids
    • A61M2202/0413—Blood
    • A61M2202/0429—Red blood cells; Erythrocytes
    • A61M2202/0437—Blood stem cells

Definitions

  • the invention relates to a medical device, in particular to a bone marrow stem cell filtration screening enrichment and rapid compounding device. Background technique
  • the autologous stem cell enrichment technology (without in vitro culture) has been directly replanted to avoid immunological rejection, and the enriched autologous stem cells can realize their directional induction and tissue regeneration using the local microenvironment.
  • the treatment of some diseases Its greatest advantage is that it does not require in vitro culture and can be performed concurrently during surgery, avoiding the technical problems of complex operations and related ethical issues, which cannot be replaced and compared by traditional methods.
  • Hematopoietic stem cell transplantation is currently used to treat hematological diseases; lymphocytes are modified by in vitro screening, co-culture and purification techniques to prevent graft versus host or adoptive immunotherapy for hematological tumors or solid tumors; enrichment and in vitro of bone marrow mesenchymal stem cells Amplification technology promotes bone and cartilage repair; transplantation of endothelial progenitor cells for coronary heart disease, dendritic cell transplantation for tumor and islet cell transplantation
  • BMSCs bone marrow stem cells
  • Animal experiments of BMSCs to promote bone repair have obtained relatively positive results, but clinical treatment is still subject to many restrictions.
  • the object of the present invention is to overcome the deficiencies of the prior art and to provide a stem cell filtration screening enrichment and rapid composite device which can achieve high concentration of bone marrow stem cells during surgery without in vitro culture.
  • a technical solution for achieving the above object is: a stem cell filtration screening enrichment and rapid composite device, comprising a filter, a two-way tee plate connected to the filter, and two ends respectively and the two-way three-way An extension tube to which the plate and the filter are attached, a blood circulation driving device provided on the extension tube, and a porous material.
  • the filter comprises a jacket and a filter casing, the jacket comprising a casing and a back cover detachably joined together, the casing having a cylindrical shape with a front end bottom wall, and the rear cover having a rear end bottom wall Cylindrical, respectively, a through hole is formed in the center of the bottom wall;
  • the filter box has a cylindrical shape, and has a mesh box cover and a mesh box bottom, and the inner cavity is a stem cell of adjustable volume Screening the enrichment chamber, which is inserted at the rear of the inner cavity of the outer casing;
  • the porous material is disposed in a stem cell screening enrichment chamber of the filter cartridge, the shape of the porous material matching the shape of the stem cell screening enrichment chamber.
  • the above stem cell filtration is selected as an enrichment and rapid composite device, wherein the porous material is allogeneic bone, collagen, degradable artificial bone or surface-modified cell scaffold material.
  • the stem cell screening enrichment chamber of the filter cartridge is adjusted in volume by an adjusting member, and the adjusting member is a length of three minutes of the length of the filter cartridge.
  • the above-described stem cell filtration screening enrichment and rapid composite device wherein the outer casing and the rear cover are detachably joined together by an engaging device, and the engaging device includes a female thread provided on an outer peripheral surface of a rear portion of the outer casing and A male thread is provided on the inner peripheral surface of the cover to be screwed to the female thread on the outer casing.
  • the stem cell filtration screening enrichment and rapid composite device described above wherein both ends of the extension tube are connected to the through hole of the filter front end and the two-way tee plate through a male luer connector.
  • the above-described stem cell filtration screening enrichment and rapid composite device wherein the blood circulation driving device may be a peristaltic pump.
  • the invention relates to the technical scheme of the enriched and rapid composite device for stem cell filtration screening, which integrates the autologous stem cell in vitro screening and enrichment and the composite device, and can realize the biological compound with high concentration of bone marrow stem cells in the operation without in vitro culture.
  • Active material which has the following characteristics:
  • the stem cell filtration screening enrichment and rapid composite device of the present invention can obtain bone marrow stem cells enriched from bone marrow blood by using different porous materials, and at the same time, after being compounded with artificial bone or other biological materials, the affected area is returned. Perform bone repair and promote bone regeneration; this method improves the efficiency of treatment and expands the scope of use;
  • the stem cell filtration screening enrichment and rapid composite device of the present invention can be used for the treatment of diseases requiring bone repair, such as bone defects, orthopedics, nonunion, spine and joints, such as orthopedics, orthopedics, neurosurgery, oral and maxillofacial surgery. Fusion, bone cyst, bone graft around the prosthesis, skull repair, etc.
  • Figure 1 is a front view of the stem cell filtration screening enrichment and rapid composite device of the present invention
  • FIG. 2 is a cross-sectional view of the filter in the stem cell filtration screening enrichment and rapid composite device of the present invention
  • FIG. 3 is a view showing the working state of the stem cell filtration screening enrichment and rapid composite device of the present invention.
  • the stem cell filtration screening enrichment and rapid composite device of the present invention comprises a filter 1, a two-way tee plate 2, and an extension a tube 3, a blood circulation driving device 4 and a porous material 5, wherein one end of the two-way tee plate 2 is connected to the rear end of the filter 1; the length of the extension tube 3 can be adjusted according to the use occasion
  • the two ends of the extension tube are respectively connected to the through hole at the front end of the filter 1 and the other end of the two-way tee plate 2 through the male luer connector 30;
  • the blood circulation driving device 4 may be a peristaltic pump; It can be locked to prevent the pressure inside the tube 3 from being too high and falling off during the peristaltic pumping process.
  • the filter 1 includes a jacket 11, a filter box 12 and an adjusting member 13, wherein the outer casing 11 includes a casing 111 and a rear cover 112 detachably coupled together, and the material of the casing 111 is PC.
  • the filter case 12 has a cylindrical shape, and its length is smaller than the length of the outer casing 111, and has a filter box cover 121 and a filter box bottom 122, the inner cavity of which is an adjustable volume stem cell screening enrichment cavity, which is inserted at the rear of the inner cavity of the outer casing 111;
  • the adjusting member 13 is used for adjusting the volume of the stem cell screening enrichment chamber of the filter cartridge 12, and the adjusting member 13 is a cylindrical shape having a length which is two-thirds to one-half of the length of the filter cartridge 12, and its outer surface A female thread is disposed on the inner peripheral surface of the filter cartridge 12, and a male screw threaded with the female thread of the adjusting member 120 is disposed to engage the adjusting member 13 in the inner cavity of the filter cartridge 12, and the bottom of the filter box 122 is located at the front end of the adjusting member 13;
  • the porous material 5 is used as a cell scaffold as a screening enrichment medium for bone marrow stem cells; the porous material 5 may be allogeneic bone, collagen, degradable artificial bone and surface-modified cell scaffold material, and its shape and screening enrichment of the filter cartridge 12 The shape of the cavity is matched and placed in the enrichment chamber of the filter cartridge 12;
  • the mesh box cover 121 and the filter box bottom 122 are respectively located at the front and rear ends of the porous material 5 for fixing the porous material 5 to prevent the porous material 5 from being The movement, and the bone marrow blood can pass smoothly.
  • the material of the filter box cover 121 and the filter box bottom 122 is PE or nylon.
  • the inner side of the rear end bottom wall of the rear cover 112 is provided with a nesting 1121 whose outer diameter matches the inner diameter of the adjusting member 13, and when the rear cover 112 is screwed onto the outer casing 111, the rear cover 112 The nesting 1121 is inserted over the rear inner cavity surface of the adjusting member 13 to stabilize the filter cartridge 12.
  • the working principle of the stem cell filtration screening enrichment and rapid composite device of the present invention is as follows:
  • a filter-like cover 121 and a filter-like case bottom 122 of a certain aperture are selected as needed.
  • the rear cover 112 on the device 1 and the filter cartridge 12 is removed to place the porous material 5 into the screening enrichment chamber, and the adjusting member 13 in the filter cartridge 12 is rotated to adjust the size of the screening enrichment chamber and to make the filter box
  • the bottom 122 compresses the porous material 5 and then reinserts the filter cartridge 12 into the outer casing 11.
  • One of the two-way tee plate 2 is opened as a blood injection port 21, the blood injection port 21 is connected to a puncture device 6 to connect the blood bag, and the other switch is also opened as the exhaust port 22, and the anti-coagulated bone marrow blood is opened.
  • the blood Slowly pushing into the extension tube 3 from the injection port 21, the blood enters the filter 1 through the extension tube 3 in the direction of the arrow in FIG. 3, and the blood inlet port 21 and the row of the two-way tee plate 2 are closed after the filter 1 is filled with blood.
  • the gas port 22 allows the blood to pass through the filter-like lid 121 located at the front end of the screening enrichment chamber of the filter cartridge 12, and is subjected to screening and enrichment of the stem cells through the porous material 5 in the screening enrichment chamber of the filter 1 and simultaneously compounding
  • the switch of the two-way tee plate 2 is opened as a recovery port, and the blood after the enrichment of the stem cells is recovered through the recovery port, and finally opened.
  • the filter 1 takes out the porous material 5 enriched for stem cells for use.
  • the present invention is a dry cell filtration screening enrichment and rapid composite device, and can obtain bone marrow stem cells enriched from bone marrow blood by using different porous materials, and can be compounded with biological porous materials such as artificial bone, and replanted in The affected area can be used for bone repair and bone regeneration.
  • the stem cell filtration screening of the present invention can be used to screen the enriched and rapid composite device without the need for cell culture in vitro, and the operation of the clinical cell therapy can be simplified and safe and effective. Emergency cases can be directly treated with enriched stem cells without waiting.

Description

一种干细胞过滤筛选富集并快速复合装置 技术领域
本发明涉及一种医疗器械， 具体涉及一种骨髓干细胞过滤筛选富集并快速 复合装置。 背景技术
近年来研究发现的自体干细胞富集技术（不经过体外培养）， 直接回植， 不但能避免免疫排斥反应， 而且富集的自体干细胞可以利用局部的微环境实现 其定向诱导和组织再生， 由此对一些疾病的治疗带来了希望。 其最大的优点是 无需体外培养， 可在手术同期进行， 避免了复杂操作的技术问题和相关的伦理 学问题， 这是传统方法无法替代和比拟的。
目前通过造血干细胞移植治疗血液系统疾病； 通过体外筛选、 共培养和純 化技术改良淋巴细胞， 防范移植物抗宿主或进行过继免疫治疗血液系统肿瘤或 实体瘤； 骨髓间充质干细胞的富集和体外扩增技术促进骨与软骨的修复； 血管 内皮祖细胞的移植治疗冠心病， 树突状细胞移植治疗肿瘤、 胰岛细胞移植治疗
I 型糖尿病、 角膜缘干细胞移植修复角膜、 神经干细胞移植治疗脑外伤和帕金 森等疾病等诸多方面的理论技术均在不断的开发研究。 由此可见， 干细胞的医 学应用的前景巨大， 是对传统治疗模式的一场革命。
随着干细胞研究的不断深入， 以骨髓干细胞（ bone marrow s tem ce l l s , BMSCs) 作为种子细胞的细胞治疗受到越来越多的关注。 BMSCs 促进骨修复的 动物实验已获得较为肯定的效果， 但进行临床治疗仍受到诸多限制。 第一， 单 純用自体骨髓血进行治疗时， 因单位体积骨髓血中 BMSCs 量少而使疗效降低。 第二， 若采用细胞体外培养扩增， 则患者要等待一段时间才能进行回植手术， 一般约需要 3 周时间，不能满足临床急诊或及时治疗的需要； 且体外培养必须 避免各种微生物的污染， 对环境和设备的要求较高， 因此增加了治疗费用； 另 外体外培养常以胎牛血清为培养基， 这也带来了临床细胞治疗的伦理学争议； 若不用体外培养即能在术中获得高浓度的 BMSCs ,则有可能提供一种新的途径， 促进细胞治疗在临床的应用。 发明内容
本发明的目的是为了克服现有技术的不足， 提供一种干细胞过滤筛选富集 并快速复合装置， 它可以实现不用体外培养即能在术中获得高浓度的骨髓干细 胞。
实现上述目的的技术方案是： 一种干细胞过滤筛选富集并快速复合装置， 包括一过滤器、 一与所述过滤器连接的两联三通板、 一两端分别与所述两联三 通板及所述过滤器连接的延长管、 一设在所述延长管上的血液循环驱动装置， 及一多孔材料。
所述过滤器包括外套及一过滤盒， 所述外套包括可拆卸地接合在一起的外 壳和后盖，所述外壳呈有前端底壁的圓筒形，所述后盖呈有后端底壁的圓筒形， 它们的底壁中央分别设有一通孔； 所述过滤盒呈一圓筒形， 它具有滤网状盒盖 和滤网状盒底， 它的内腔为可调节体积大小的干细胞筛选富集腔， 它插置在所 述外壳的内腔后部；
所述多孔材料安置在所述过滤盒的干细胞筛选富集腔中， 该多孔材料的形 状与干细胞筛选富集腔的形状匹配。
上述的干细胞过滤歸选富集并快速复合装置， 其中， 所述多孔材料为异体 骨、 胶原、 可降解人工骨或经表面修饰的细胞支架材料。
上述的干细胞过滤筛选富集并快速复合装置， 其中， 所述过滤盒的干细胞 筛选富集腔是通过一调节件来调节体积大小的， 该调节件为一长度是所述过滤 盒长度的三分之二至二分之一的圓筒形， 它的外表面上设有阴螺紋， 所述过滤 盒的内周面上设有与所述调节件的阴螺紋相螺合的阳螺紋， 以使所述调节件接 合在所述过滤盒的内腔中， 所述滤网状盒底位于所述调节件的前端。
上述的干细胞过滤筛选富集并快速复合装置， 其中， 所述外壳和所述后盖 是通过接合装置可拆卸地接合在一起的， 该接合装置包括设在外壳后部外周面 上的阴螺紋及设在盖的内周面上的与所述外壳上的阴螺紋相螺合的阳螺紋。 上述的干细胞过滤筛选富集并快速复合装置， 其中， 所述延长管的两端通 过公鲁尔接头与所述过滤器前端的通孔和所述两联三通板连接。
上述的干细胞过滤筛选富集并快速复合装置， 其中， 所述血液循环驱动装 置可以是蠕动泵。
本发明的干细胞过滤筛选富集并快速复合装置的技术方案， 它集自体干细 胞体外筛选富集、 复合材料装置于一体， 可以实现不用体外培养即能在术中获 得复合有高浓度骨髓干细胞的生物活性材料， 它具有以下特点：
(1)采用全封闭、 一次性管路， 全部操作在手术室内完成， 保证了无菌、 安全、 无交叉感染；
(2)本发明的干细胞过滤筛选富集并快速复合装置， 通过采用不同的多孔 材料， 能从骨髓血中筛选获得富集骨髓干细胞， 同时与人工骨或其他生物材料 复合后， 回植患处， 进行骨修复、促进骨再生； 该种方法提高了治疗的有效率， 拓展了使用范围；
(3)采用本发明的干细胞过滤筛选富集并快速复合装置， 无须进行细胞体 外培养， 急诊的病例无需等待即可直接进行富集干细胞治疗；
(4)本发明的干细胞过滤筛选富集并快速复合装置可用于骨科、 整形外科、 神经外科、 口腔颌面外科等常见需要骨修复的疾病的治疗， 如骨缺损、骨不连、 脊柱和关节融合、 骨囊肿、 假体周围植骨、 面颅骨修复等。 附图说明
图 1为本发明的干细胞过滤筛选富集并快速复合装置的主视图；
图 2为本发明的干细胞过滤筛选富集并快速复合装置中过滤器的剖视图； 图 3为本发明的干细胞过滤筛选富集并快速复合装置的工作状态图。 具体实施方式
下面通过具体地实施例并结合附图对本发明的技术方案进行说明： 请参阅图 1 , 本发明的干细胞过滤筛选富集并快速复合装置， 包括一过滤 器 1、 两联三通板 2、 延长管 3、 血液循环驱动装置 4及多孔材料 5 , 其中， 两 联三通板 2的一端与过滤器 1后端连接； 延长管 3的长度可以根据使用场合调 接； 延长管的两端分别通过公鲁尔接头 30与过滤器 1前端的通孔和两联三通 板 2的另一端连接； 血液循环驱动装置 4可以是蠕动泵； 公鲁尔接头 30最好 能够锁定， 防止在蠕动泵在蠕动加压的过程中延长管 3内压力过高而脱落。
再请参阅图 2 , 过滤器 1包括外套 11、 一过滤盒 12及一调节件 13 , 其中， 外套 11包括可拆卸地接合在一起的一外壳 111和一后盖 112 ,外壳 111的 材料为 PC (聚碳酸酯）， 它呈有前端底壁的圓筒形， 后盖 112呈有后端底壁的 圓筒形， 它们的底壁中央分别设有一通孔 1110、 1120; 外壳 111的后部外周面 上设有阴螺紋，后盖 112的内周面上设有与外壳 111的阴螺紋相螺合的阳螺紋； 过滤盒 12呈一圓筒形， 它的长度小于外壳 111的长度， 它具有滤网状盒 盖 121和滤网状盒底 122 , 它的内腔为可调节体积大小的干细胞筛选富集腔， 它插置在外壳 111的内腔后部；
调节件 13用来调节过滤盒 12的干细胞筛选富集腔的体积大小， 该调节件 13为一长度是过滤盒 12长度的三分之二至二分之一的圓筒形， 它的外表面上 设有阴螺紋， 过滤盒 12的内周面上设有与调节件 120的阴螺紋相螺合的阳螺 紋， 以使调节件 13接合在过滤盒 12的内腔中， 滤网状盒底 122位于调节件 13 的前端；
多孔材料 5作为细胞支架用作骨髓干细胞的筛选富集媒介； 多孔材料 5可 以为异体骨、 胶原、 可降解人工骨及经表面修饰的细胞支架材料， 它的形状与 过滤盒 12的筛选富集腔的形状匹配并被安置在过滤盒 12的富集腔中；
当多孔材料 5放置在过滤盒 12的筛选富集腔中时， 滤网状盒盖 121和滤 网状盒底 122分别位于多孔材料 5的前后端， 用以固定多孔材料 5 , 防止多孔 材料 5的移动， 并且可使骨髓血液顺利通过。
滤网状盒盖 121和滤网状盒底 122的材料为 PE或尼龙。
在一个实施例中， 后盖 112 的后端底壁内侧面上设有一外径与调节件 13 的内径匹配的嵌套 1121 , 当后盖 112旋合在外壳 111上时， 后盖 112上的嵌套 1121插套在调节件 13的后部内腔面上， 以稳定过滤盒 12。
再请参阅图 3 , 本发明的干细胞过滤筛选富集并快速复合装置的工作原理 疋：
根据需要选择一定孔径的滤网状盒盖 121和滤网状盒底 122。 先旋下过滤 器 1上的后盖 112 , 并卸下过滤盒 12将多孔材料 5放入筛选富集腔中， 旋转过 滤盒 12内的调节件 13 , 可以调节筛选富集腔的大小并使滤网状盒底 122压紧 多孔材料 5 , 然后重新将过滤盒 12装入外套 11中。 打开两联三通板 2中的一 个开关作为血液注入口 21 , 该血液注入口 21连接一穿刺器 6以连接血液袋， 另一个开关也打开作为排气口 22 , 把带抗凝的骨髓血从注入口 21緩慢推入延 长管 3 , 血液沿如图 3中的箭头方向通过延长管 3进入过滤器 1 , 过滤器 1注 满血液后关闭两联三通板 2的血液注入口 21及排气口 22 , 使血液经过位于过 滤盒 12的筛选富集腔的前端的滤网状盒盖 121后， 在过滤器 1的筛选富集腔 中通过多孔材料 5进行干细胞的筛选富集并同时复合生物材料， 经过约数十次 循环完成对骨髓干细胞的筛选富集后， 打开两联三通板 2的其中的一个开关作 为回收口， 使经过干细胞富集后的血液经回收口回收， 最后打开过滤器 1将富 集干细胞的多孔材料 5取出备用。
由此可见， 本发明是干细胞过滤筛选富集并快速复合装置， 可以通过采用 不同的多孔材料， 从骨髓血中筛选获得富集骨髓干细胞， 同时能与人工骨等生 物多孔材料复合， 回植于患处， 可以进行骨修复、 促进骨再生。 采用本发明的 干细胞过滤筛选富集并快速复合装置， 无需进行细胞体外培养， 可在手上同期 进行， 简化临床细胞治疗的操作， 安全有效。 急诊的病例无需等待即可直接进 行富集干细胞治疗。
本技术领域中的技术人员应当认识到， 以上的实施例仅是用来说明本发 明， 而并非用作为对本发明的限定， 只要在本发明的实质精神范围内， 对以上 所述实施例的变化、 变型都将落在本发明的权利要求书范围内。

Claims

1. 一种干细胞过滤歸选富集并快速复合装置， 包括一过滤器、 一与所述 过滤器连接的两联三通板、 一两端分别与所述两联三通板及所述过滤器连接的 延长管及一设在所述延长管上的血液循环驱动装置， 其特征在于， 所述复合装 置还包括一多孔材料， 所述过滤器包括外套及一过滤盒，
所述外套包括可拆卸地接合在一起的外壳和后盖， 所述外壳呈有前端底壁 的圓筒形，所述后盖呈有后端底壁的圓筒形，它们的底壁中央分别设有一通孔； 所述过滤盒呈一圓筒形， 它具有滤网状盒盖和滤网状盒底， 它的内腔为可 调节体积大小的干细胞筛选富集腔， 它插置在所述外壳的内腔后部；
所述多孔材料安置在所述过滤盒的干细胞筛选富集腔中， 该多孔材料的形 状与干细胞筛选富集腔的形状匹配。
2. 根据权利要求 1 所述的干细胞过滤筛选富集并快速复合装置， 其特征 在于， 所述多孔材料为异体骨、 胶原、 可降解人工骨或经表面修饰的细胞支架 材料。
3. 根据权利要求 1 所述的干细胞过滤筛选富集并快速复合装置， 其特征 在于， 所述过滤盒的干细胞筛选富集腔是通过一调节件来调节体积大小的， 该 调节件为一长度是所述过滤盒长度的三分之二至二分之一的圓筒形， 它的外表 面上设有阴螺紋， 所述过滤盒的内周面上设有与所述调节件的阴螺紋相螺合的 阳螺紋， 以使所述调节件接合在所述过滤盒的内腔中， 所述滤网状盒底位于所 述调节件的前端。
4. 根据权利要求 1 所述的干细胞过滤筛选富集并快速复合装置， 其特征 在于， 所述外壳和所述后盖是通过接合装置可拆卸地接合在一起的， 该接合装 置包括设在外壳后部外周面上的阴螺紋及设在盖的内周面上的与所述外壳上 的阴螺紋相螺合的阳螺紋。
5. 根据权利要求 1 所述的干细胞过滤筛选富集并快速复合装置， 其特征 在于， 所述延长管的两端通过公鲁尔接头与所述过滤器前端的通孔和所述两联 三通板连接。
6. 根据权利要求 1 所述的千细胞过滤筛选富集并快速复合装置， 其特征 在于， 所述血液循环驱动装置可以是蠕动泵。
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