WO2011146394A1 - Stimulus responsive polymers for the purification of biomolecules - Google Patents
Stimulus responsive polymers for the purification of biomolecules Download PDFInfo
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- WO2011146394A1 WO2011146394A1 PCT/US2011/036648 US2011036648W WO2011146394A1 WO 2011146394 A1 WO2011146394 A1 WO 2011146394A1 US 2011036648 W US2011036648 W US 2011036648W WO 2011146394 A1 WO2011146394 A1 WO 2011146394A1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/08—Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/12—Macromolecular compounds
- B01J41/14—Macromolecular compounds obtained by reactions only involving unsaturated carbon-to-carbon bonds
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F26/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen
- C08F26/02—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen by a single or double bond to nitrogen
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/08—Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/12—Macromolecular compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/08—Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/12—Macromolecular compounds
- B01J41/13—Macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/32—Extraction; Separation; Purification by precipitation as complexes
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F126/00—Homopolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen
- C08F126/02—Homopolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen by a single or double bond to nitrogen
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F26/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen
- C08F26/02—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen by a single or double bond to nitrogen
- C08F26/04—Diallylamine
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F8/00—Chemical modification by after-treatment
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F8/00—Chemical modification by after-treatment
- C08F8/02—Alkylation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
Definitions
- the present invention relates to polymers useful for protein purification.
- the present invention relates, at least in part, to stimulus responsive polymers useful for the purification of a target molecule from a sample containing the target molecule and one and more impurities.
- separation and/or purification processes that are currently being used for proteins, include at least the following steps: cell lysis to recover an intracellular protein or recovery of a protein from the media in case of a secreted protein; removal of cells and cellular debris using differential centrifugation or filtration to obtain a clarified sample containing the protein of interest; and use of a variety of chromatography media in a multi-step process to separate a protein of interest from the various impurities in the sample.
- polymers including polyelectrolytes
- polyelectrolytes have been employed in one or more steps for the purification of biomolecules, especially proteins.
- polyelectrolytes in flocculation to purify proteins is well established (see, e.g., International PCT Patent Application No. WO2008/091740). This can be accomplished with a wide range of polymers, with the only required general characteristic being the polymer must have some level of interaction with a species of interest (e.g., a target molecule or an impurity).
- species of interest e.g., a target molecule or an impurity
- the most common methodology is the use of polymers containing ion species, such as polyelectrolytes.
- polyelectrolytes are added to the protein mixture and purification is achieved via selective flocculation of one or more components of the mixture.
- a critical drawback of this approach is that carefully controlled levels of polyelectrolytes have to be added in order to avoid residual polymer contamination (e.g., when polymer level too high) or inefficient flocculation (e.g., when polymer level too low). Because ion exchange and other charged chromatography media are commonly used in the purification of proteins, residual polyelectrolytes can potentially bind to the media used in downstream purification steps, thereby fouling and complicating the process.
- the present invention provides novel polyelectrolyte based stimulus responsive polymers which are easily scalable and operate over a wide range of pH and conductivity, thereby enabling their use in the purification of a wide array of biomolecules including, e.g., therapeutic proteins.
- a stimulus responsive polymer comprising a polyelectrolyte backbone comprising one or more hydrophobic groups, where the polymer is capable of binding and precipitating a biomolecule of interest in a sample following the addition of a stimulus.
- the polyelectrolyte backbone of a polymer according to the present invention comprises at least two monomelic units or at least three monomelic units. In some embodiments, at least 50% of the monomelic units comprise a charge. In other embodiments, each monomelic unit of the polyelectrolyte backbone comprises a charge.
- a stimulus responsive polymer according to the present invention comprises a polyamine backbone.
- one or more hydrophobic groups is a phenyl group.
- the stimulus responsive polymers according to the present invention are useful for purifying a desired target molecule and do so by separating the desired target molecule from one or more undesirable entities present in a sample along with the desired target molecule.
- a stimulus responsive polymer according to the present invention binds and precipitates a biomolecule of interest, which itself is the desirable target molecule bound and precipitated by the stimulus responsive polymer.
- a stimulus responsive polymer binds and precipitates a biomolecule of interest, which is an undesirable entity present in a sample along with the desirable target molecule.
- the biomolecule of interest is a therapeutic polypeptide (i.e., the desirable target molecule).
- the therapeutic polypeptide is an antibody (e.g., a monoclonal antibody).
- the biomolecule of interest is selected from the group consisting of host cell protein, DNA, R A, lipids, viruses, endotoxins, cell culture additives, whole cells and cellular debris.
- a polymer according to the present invention is responsive to a stimulus which is a complex forming salt.
- the stimulus responsive polymers are unique and inventive over the polymers described in the prior art, in that they supplant or improve upon one or more steps in a purification process, thereby to substantially increase the overall purity of a target molecule desired to be purified or separated from one or more undesirable entities.
- a method of increasing the purity of a target molecule comprises the steps of: (a) providing a sample comprising a target molecule and one or more impurities; (b) contacting the sample with a stimulus responsive polymer comprising a polyelectrolyte backbone comprising one or more hydrophobic groups attached to the backbone under a first set of conditions suitable for the polymer to bind the target molecule in solution, thereby to form a complex of polymer and the target molecule; and (c) adding a stimulus to the sample under a second set of conditions suitable to precipitate the complex out of solution, where the precipitation of the complex results in the separation of the target molecule from one or more impurities, thereby to increase the purity of the target molecule.
- the method further comprises the step of recovering a target molecule from the complex.
- a stimulus responsive polymer according to the present invention binds and precipitates one or more impurities, instead of the target molecule, where the precipitation of a complex of the polymer and one or more impurities results in the separation of the target molecule from the one or more impurities, thereby to increase the purity of the target molecule.
- such a method comprises the steps of: (a) providing a sample comprising a target molecule and one or more impurities; (b) contacting the sample with a stimulus responsive polymer comprising a polyelectrolyte backbone comprising one or more hydrophobic groups attached to the backbone under a first set of conditions suitable for the polymer to bind the one or more impurities, thereby to form a complex of polymer and the one or more impurities; and (c) adding a stimulus to the sample under a second set of conditions suitable to precipitate the complex, where the precipitation of the complex results in the separation of the target molecule from one or more impurities, thereby to increase the purity of the target molecule.
- a stimulus responsive polymer according to the present invention comprises the following structure:
- R 1 and R 2 are charged groups which form a part of the polyelectrolyte backbone (B); and R 3 is a hydrophobic group attached to a charged group in the backbone.
- the ratio of y monomelic units (i.e., having a hydrophobic group attached to the backbone) to the total number of monomelic units (i.e. , sum of x and y monomelic units) represents the "percent of hydrophobic modification" of the polymer.
- a stimulus responsive polymer according to the present invention comprises the following structure:
- x, y and z are monomelic units in the polymer
- R 1 , R 2 and R 3 are charged groups which form a part of the polyelectrolyte backbone (B);
- R4 is a hydrophobic group attached to a charged group in the backbone;
- Rs is a functional group attached to a charged group in the backbone.
- the ratio of y monomelic units (i.e., having hydrophobic group attached to backbone) to the total number of monomelic units in the polymer (i.e., sum of x, y and z monomelic units) represents the "percent hydrophobic modification" of the polymer.
- the ratio of z monomelic units (i.e., having a functional group attached to a charged group on the backbone) to the total number of monomelic units (i.e., sum of x, y and z units) represents the "percent functional group modification" of the polymer.
- a polymer encompassed by the present invention may have "n" number of any of monomelic units x, y or z, described herein, where n is equal or greater than two.
- a stimulus responsive polymer according to the present invention comprises the following structure:
- R 1 , R 2 are aliphatic amine groups (e.g., primary or secondary and/or aromatic amines) which form a part of a carbon containing backbone of a polyelectrolyte; and R 3 is a hydrophobic group attached to the amine group R 2 and contains 4 or more carbon atoms (e.g., an alkyl group, an alkenyl group, an aralkenyl group or a fluorocarbon group).
- the ratio of y (i.e., monomelic units having a hydrophobic group attached to a charged group in the polyelectrolyte backbone) to x (i.e., unmodified charged group in the polyelectrolyte backbone) is 0.01 to 0.75 or 0.05 to 0.75. Accordingly, the percent hydrophobic group modification would be between 1% to 75% or between 5% to 75% of the total polyelectrolyte monomelic units (i.e., x + y).
- a stimulus responsive polymer according to the present invention comprises the following structure:
- R 1 , R 2 and R 3 are aliphatic amine groups which form a part of the carbon containing polyelectrolyte backbone (e.g., primary or secondary amines and/or aromatic amines);
- R 4 is a hydrophobic group containing 4 or more carbon atoms and selected from alkenyl, aralkyl and aralkenyl groups; and
- R 5 is a hydrophobic group containing 4 or more 4 carbon atoms and selected from an alkyl or a fluorocarbon group.
- T e ratio of y monomelic units to the total number of polyelectrolyte monomelic units is between 0.01 and 0.75.
- the ratio of z monomelic units to the total number of polyelectrolyte monomelic units is between 0.05 and 0.5 or between 0.01 and 0.5. Accordingly, the percent hydrophobic group modification is between 1 % and 75% or between 5% and 75% and the percent functional group modification is between 1% and 50% or between 5% and 50%.
- Additional methods of using the stimulus responsive polymers according to the present invention include methods which enable the purification of a target molecule or product of interest (e.g., an antibody) while minimizing the amount of residual polymer in the sample.
- a method of separating a target molecule (e.g., an antibody) from one or more impurities using a stimulus responsive polymer according to the present invention while minimizing residual amounts of polymer comprises the steps of: (a) providing a sample comprising a target molecule and one or more impurities; (b) contacting the sample with a stimulus responsive polymer under a first set of conditions suitable for the polymer to bind the one or more impurities, thereby to form a first complex of polymer and one or more impurities, where the first set of conditions comprise adjusting pH or salt concentration of the sample before or after the addition of the polymer; (c) precipitating the first complex from the sample under a second set of conditions; (d) contacting the sample with
- the target molecule is an antibody.
- the antibody is a monoclonal antibody.
- recovering the target molecule in various methods according to the present invention comprises a chromatography step.
- recovering the target molecule comprises a filtration step.
- methods according to the present invention may include two or more steps which employ a stimulus responsive polymer according to the invention.
- a stimulus responsive polymer may be used to precipitate one or more impurities in one step of the purification process and same or different polymer may be used to precipitate a target molecule or the desired product in a different step of the process.
- the one or more impurities is selected from the group consisting of host cell protein, DNA, R A, antibody aggregates, viruses, endotoxins, whole cells, cellular debris and cell culture additives.
- Figure 1 is a schematic depicting the reaction of a polyallylamine polymer with benzylchloride
- Figure 2 depicts the reaction scheme for hexanoic acid and tert-butyl modified polyallylamine (HC-t-BuMPAA)
- Figure 3 is a graph depicting the effect of sodium chloride on the multivalent ion stimulus and pH responsiveness of hexanoic acid and tert-butyl modified polyallylamine (HC-t-BuMPAA).
- the X-axis depicts pH and Y-axis depicts turbidity of the centrate (i.e., output of a centrifuge).
- Figure 4 depicts the synthesis of polyvinylamine, as described in
- Figure 5 depicts a reaction for the deprotection of polyamine after polymerization.
- Figure 6 depicts a reaction of polyvinylamine with benzylchloride .
- Figure 7 depicts a polymerization and reaction scheme to form a multivalent ion stimulus responsive copolymer.
- Figure 8 depicts an NMR spectrum for modified polyvinylamine
- Figure 9 depicts an NMR spectrum for modified polyallylamine from
- FIG. 10 depicts a graph demonstrating the effect of polymer dosing for a non-stimulus responsive polymer (e.g., chitosan) and a stimulus responsive polymer (e.g., benzyl modified polyallylamine) on centrate turbidity.
- the X-axis is the polymer dose (wt%) and the Y-axis is the centrate turbidity (NTU), as described in Example 37.
- Figure 11 depicts a graph demonstrating the effect of polymer dose in the presence and absence of a stimulus for a benzyl polyallylamine stimulus responsive polymer.
- the X-axis is the polymer dose in wt% and Y-axis is the centrate turbidity (NTU), as described in Example 38.
- Figure 12 depicts a typical scheme used for the purification of biomolecules.
- Figure 13 depicts a purification scheme including a stimulus responsive polymer used to improve the clarification of the cell culture.
- the stimulus responsive polymer removes one or more impurities, however, the polymer does not bind the desired target molecule.
- Figure 14 depicts a purification scheme including a stimulus responsive polymer used to improve the clarification of the cell culture.
- the stimulus responsive polymer removes one or more impurities via flocculation, however, the polymer does not bind the desired target molecule and the residual polymer is removed by adding a stimulus after clarification.
- Figure 15 depicts a purification scheme including a stimulus responsive polymer used to improve the clarification of the cell culture.
- the stimulus responsive polymer removes one or more impurities, however, the polymer does not bind the target molecule and the residual polymer is removed by an additional adsorptive filtration step following clarification.
- the present invention provides, at least in part, a novel and improved stimulus responsive polymer comprising a polyelectrolyte backbone modified with one or more hydrophobic groups, where the polymer solubility can be altered by the addition of a stimulus.
- the stimulus responsive polymers and methods of using the same described herein are more efficient than those described in the prior art, in that, they provide an improved range of pH for purification of desired target molecules, including, e.g., proteins, as well as removal of undesirable entities such as impurities, e.g., host cell proteins, DNA, R A, lipids, endotoxins, cell culture additives, cells and cellular debris.
- the polymers described herein are responsive to low concentrations of simple multivalent salts, thereby allowing for improved scalability and reduced conductivity relative to existing salt responsive polymers.
- the polymers according to the present invention can effectively remove whole cells, cellular debris as well as other soluble impurities from cell culture media.
- the polymers can also effectively remove impurities from protein mixtures containing a protein of interest and one or more impurities. Further, various polymers described herein are able to effectively capture target molecules and proteins/products of interest in a sample, thereby to separate them from one or more impurities present in the sample and increase the purity of the target molecule.
- Also encompassed by the present invention are methods of using the polymers described herein for purification of target molecules, e.g., therapeutic proteins, using a wide array of conditions.
- the stimulus responsive polymers described herein can be used for the binding and precipitation of either a desired target molecule, e.g., a therapeutic protein or a desirable product, or an undesired entity, e.g., one or more impurities including, e.g., host cell protein, DNA, RNA, lipids, endotoxins, cell culture additives, whole cells and cellular debris.
- a desired target molecule e.g., a therapeutic protein or a desirable product
- an undesired entity e.g., one or more impurities including, e.g., host cell protein, DNA, RNA, lipids, endotoxins, cell culture additives, whole cells and cellular debris.
- a biomolecule of interest whether it is the desired target molecule or an undesired entity.
- the selection of a particular stimulus responsive polymer to use, as described herein, is determined based on what the polymer is intended to bind. For example, in case of a biomolecule of interest which possesses a net negative charge at a pH above its pi (e.g., whole cells, cellular debris, DNA, endotoxins, and proteins), a stimulus responsive polymer comprising a polyelectrolyte backbone which is cationic (i.e., positively charged) is desirable to use. On the other hand, in case of a biomolecule of interest which comprises a net positive charge at a pH below its pi (e.g. proteins), a stimulus responsive polymer comprising a polyelectrolyte backbone which is anionic (i.e., negatively charged) is desirable to use.
- a positive charge could be inherent to the polymer under conditions used during the purification process or the positive charge can be generated with a change in pH which renders the stimulus responsive polymer charged.
- An important parameter affecting the overall recovery of a biomolecule is the ratio of hydrophobic modification groups to the remaining unmodified charged groups in the polyelectrolyte backbone. For example, as the percentage of hydrophobic groups increases so does the resulting loss of the biomolecule through non-specific interactions. Therefore, for a given biomolecule, a specific ratio of charged groups to hydrophobic groups can be used in order to maximize biomolecule recovery.
- a high percentage of hydrophobic groups may limit polymer solubility and the effectiveness of charged groups on the polyelectrolyte backbone.
- modification of a charged amine group in the backbone of the polyelectrolyte polyallylamine with benzyl chloride results in a secondary amine which is charged under a wide array of pH conditions.
- benzyl modification adds a steric bulk to the amine group which can affect charge-charge interactions.
- modification of a charged group in a polyelectrolyte backbone with a hydrophobic group can result in a reduction in the number of charged groups.
- modification of an amine group of polyallylamine with benzyl chloride results in the formation of an amide linkage which is not a charged group, thereby resulting in a reduction in the number of charged groups in the backbone. Accordingly, a reduction in the number of charged groups can also affect both the polymer's solubility as well as the polymer's ability to bind through charge-charge interactions.
- hybrid polymers may also be synthesized, which comprise a polyelectrolyte backbone which is cationic and modified with one or more hydrophobic as well as anionic groups.
- unmodified groups on the cationic polyelectrolyte are responsive to a complex forming salt, while the anionic modification groups on the backbone can bind a biomolecule of interest which possesses a net positive charge.
- the ratio of unmodified cationic groups in the polyelectrolyte backbone to anionic and hydrophobic groups is important to determine the solubility and stimulus necessary for the polymer to complex and capture the biomolecule of interest. For example, too few unmodified cationic groups in the polyelectrolyte backbone can result in limited to no response to stimulus. Whereas, too few anionic groups can limit the ability to capture a biomolecule of interest.
- the requisite level of modification of polyelectrolyte backbone by hydrophobic groups as well as the type and amount of stimulus that is used can be determined based on the biomolecule of interest to be purified using the polymer as well as the conditions used, and the inherent solubility and molecular weight of the polymer backbone. For example, in order to minimize the amount of stimulus used, e.g., a multivalent salt, it is desirable to have more hydrophobic groups attached to the polyelectrolyte backbone. Alternatively, increased amount of multivalent ion stimulus reduces the extent of the hydrophobic modification or percent hydrophobic
- hydrophobic modification required. In some instances, it may be possible to completely eliminate the need for hydrophobic modification, for example, if the multivalent ion stimulus is at a very high concentration. Alternatively, increasing the polymer molecular weight reduces the inherent solubility of the polyelectrolyte backbone and may allow for low (5 % or less) or no polyelectrolyte hydrophobic modification. However, the elimination of hydrophobic modification can increase the amount of residual polymer for lower molecular weight or more soluble polymer backbones at high polymer doses.
- the extent of hydrophobic modification on a polyelectrolyte backbone ranges from 1% to 85% or 5% to 50%. Accordingly, depending on the biomolecule of interest to be bound by a stimulus responsive polymer, the percentage of hydrophobic modification is at least 1%, 2%, 3%, 4%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80% or 85%.
- a complex forming salt is used as a stimulus.
- the concentration of the complex forming salt ranges from 2mM to 500mM, or from 25mM to lOOmM.
- Exemplary complex forming salts include but are not limited to multivalent ions such as, e.g., citrate, phosphate, sulfate and EDTA, and ion-association salts such as perchlorate, dodecyl sulfate sodium salt, dodecyl benzene sulfate, Fe(II)-4-chloro-2-nitrophenol anion, tetraphenyl borate sodium salt and hexanitrodiphenol amine (see.
- multivalent ions such as, e.g., citrate, phosphate, sulfate and EDTA
- ion-association salts such as perchlorate, dodecyl sulfate sodium salt, dodecyl benzene sulfate, Fe(I
- the amount of a complex forming salt which is required to induce precipitation depends on factors such as, e.g., pH, polymer concentration and concentration of biomolecule of interest in a sample. For example, some
- polyelectrolytes such as polyallylamine have a charge density which varies with pH (level of amine protonation). As the pH is increased, the level of charge density is reduced so the degree of stimulus required to induce precipitation will be different than at a lower pH or higher charge density state.
- a stimulus responsive polymer according to the present invention maybe added to a feedstock containing a target molecule or a target molecule containing sample, in the form of a solid or in the form of a liquid.
- the final polymer concentration is generally between 0.01% and 2%.
- a mixture of a polymer and a biomolecule of interest is generated followed by the addition of a stimulus, e.g., a complex forming salt such as a multivalent anion.
- the amount of stimulus may depend on the polymer concentration. For example, a polymer concentration of 2% will require a higher amount of stimulus required to induce polymer precipitation. It is important that the stimulus is applied in the correct or in a slight excess amount in order to ensure complete polymer precipitation through the stimulus response. This is in contrast to polymer flocculation, where overdosing leads to problematic residual polymer.
- the present invention can be used in a variety of purification schemes.
- the stimulus responsive polymer can be beneficial at any step of the process, although the preferred use is at the beginning of the process during clarification or during capture of the target molecule.
- a single stimulus responsive polymer or mixture of polymers can be added in one or more steps and subsequently precipitated using one or more stimuli.
- the stimulus can be applied before, during or after the polymer associates with the biomolecule of interest (i.e., one or more impurities or a desired target molecule). Also, the stimulus can be applied before, during or after the removal of the precipitate, which is generally in solid form.
- the precipitate can be subsequently removed using one or more techniques known in the art and/or those described herein such as, e.g., filtration, settling, centrifugation or any other solid/liquid separation method or a combination of methods in simultaneous, parallel or series separation schemes.
- Addition of the stimulus responsive polymer can be accomplished in several ways.
- the cell culture media may be adjusted to a desired condition prior to the addition of a stimulus responsive polymer, for example adjustment (e.g., reduction) of pH and/or conductivity.
- the stimulus responsive polymer can then be added to the cell culture media and mixed.
- the stimulus responsive polymer can be added in a liquid or a solid format.
- the polymer containing solution itself can be formulated just that it adjusts the pH of the cell culture media to a desired condition.
- the stimulus responsive polymer can be dissolved in a concentrated acetic acid solution.
- the concentration of this acetic acid solution can be altered based on the volume, fermentation solution condition and protein concentration in order to provide the necessary pH adjustment upon addition of the stimulus responsive polymer, thereby to result in the desired polymer concentration and solution pH.
- the stimulus responsive polymer can be added in concentrations where spontaneous flocculation occurs, typically in the range of 0.01 to 0.1% wt polymer or 0.01 to 0.5 % wt polymer, depending on the type and percent solids, such that the solution becomes cloudy and begins to form a precipitate.
- the stimulus responsive polymer can be added in concentrations where spontaneous flocculation does not occur but polymer- biomolecule association does occur, e.g., typically, in the range of 0.5% to 2% wt polymer, and the solution could be clear or slightly cloudy or more turbid than the original solution.
- the stimulus responsive polymer can be added in concentrations where a mixture of spontaneous flocculation and polymer-biomolecule association occurs.
- a stimulus responsive polymer according to the present invention is added to the cell culture after fermentation is complete and polymer is formulated to bind a biomolecule of interest which is not the desired target molecule.
- a stimulus responsive polymer is added to the cell culture under a first set of conditions, e.g., conditions which may be adjusted before, during or after the addition of the polymer which binds the biomolecule of interest.
- a stimulus is added under a second set of conditions, thereby generating a precipitate which includes the biomolecule of interest (e.g., one or more impurities such as cells, cellular debris, host cell proteins, DNA, endotoxins, and viruses).
- the solid precipitate can be subsequently removed by centrifugation and/or filtration, thereby resulting in a clarified cell culture fluid.
- the resulting clarified cell culture fluid may be subsequently passed through a capture step using a chromatography media to bind the desired target molecule.
- the target molecule may subsequently be eluted from the capture step. Accordingly, in some cases, by using a stimulus responsive polymer according to the present invention, which enables removal of one or more impurities at the clarification step, number of additional steps may be reduced eliminated or modified.
- a stimulus responsive polymer is added to the cell culture under conditions where the polymer binds a biomolecule of interest, which is not the target molecule.
- a flocculate which includes the biomolecule of interest is allowed to form.
- the solid containing the biomolecule of interest is removed by primary clarification.
- the resulting cell culture fluid is collected and a stimulus is applied to precipitate residual polymer.
- the precipitated residual polymer is subsequently removed by secondary clarification.
- the resulting clarified cell culture solution is passed through a capture step using a chromatography media to bind the target molecule. It may be possible to remove the residual polymer using the addition of a stimulus at any of the purification steps following primary clarification. Also, it may be possible to add a stimulus to remove residual polymer at any step or add one or more stimuli multiple times throughout the process.
- a stimulus responsive polymer is added to the cell culture fluid after fermentation is complete under conditions suitable for the polymer to bind one or more impurities, such that the polymer does not bind the target molecule.
- a stimulus is added which forms a solid precipitate with one or more impurities.
- the solid precipitate is removed by centrifugation and/or filtration.
- the resulting clarified cell culture solution is passed through a filter which is capable of binding the stimulus responsive polymer.
- a filter which can bind the stimulus responsive polymer For example, a filter with similar properties to the biomolecule of interest which is to be bound by the polymer may be provided. Alternatively, a filter possessing a charged group that has the same charge as the biomolecule of interest and a charge opposite that of the stimulus responsive polymer may be used.
- Membranes, packed beds or filters may also be used to remove a polymer from solution.
- an anionic membrane containing sulfonic acid groups could be used to remove a polyamine stimulus responsive polymer.
- a filter with binding properties similar to the stimulus used to precipitate the polymer can be used. If the stimulus is a multivalent anion, then providing a filter with a surface that contains multivalent anions can remove the polymer from solution.
- a membrane modified with polyvinylphosphate could bind to polyamines in much the same way that phosphate ions can complex with polyamines, thereby to induce precipitation.
- beads (e.g., polymethacrylate) modified with polyvinylphosphate could be used.
- the resulting solution is passed through a capture step using a chromatography media to bind the target biomolecule. It is possible to remove the polymer using a chromatography media, depth filter or other porous material that can bind the stimulus responsive polymer. It is also possible to remove the polymer with adsorptive means at a single step or more than one or multiple steps. Further, it is possible to remove the polymer via adsorptive means at any step after the polymer's addition to the mixture.
- the stimulus responsive polymer can be used to substitute or enhance both the clarification and capture steps.
- two separate stimulus responsive polymers could be used; the first polymer which binds one or more impurities but does not bind the target molecule and a second polymer which binds the target molecule. These two polymers could be applied in separate steps or a single step.
- a single polymer could be used which has functional groups capable of binding the target molecule and a polyelectrolyte backbone capable of binding one or more impurities. The single polymer could bind the target molecule and one or more impurities in a single step or multiple steps.
- Subsequent elution of the target molecule from the polymer may occur after several different precipitation stimulus additions or washing steps.
- the stimulus responsive polymer can be added after the capture step and used to clarify suspended solids and impurities which result from virus inactivation or other steps after the capture step.
- the stimulus responsive polymer can also substitute for or enhance polishing steps.
- a virus inactivation step i.e., exposure of solution to low pH, surfactants or heat
- the solution conditions may be adjusted and the sample processed through a series of polishing steps (i.e., one or more of ion exchange, hydrophobic interaction, mixed mode and others).
- the solution may then undergo a series of filtration steps, including virus filtration and ultrafiltration or diafiltration.
- stimulus or “stimuli,” as used interchangeably herein, is meant to refer to a physical or chemical change in the environment which results in a response by a stimulus responsive polymer according to the present invention. Accordingly, the present invention provides novel polymers which are responsive to a stimulus and which stimulus results in a change in the solubility of the polymer.
- a stimulus comprises addition of a complexing agent or a complex forming salt to a sample.
- a stimulus is generally added after the addition of a polymer to a sample. Although, the stimulus may also be added during or before addition of a polymer to a sample.
- polymer refers to a molecule formed by covalent linkage of two or more monomer units. These monomer units can be synthetic or occur in nature.
- the polymers formed by the repeating units can be linear or branched. Examples of polymers include, but are not limited to, polyethylene glycol, polypropylene glycol, polyethylene, polyallylamine, polyvinylalcohol, polystyrene and copolymers (e.g. polystyrene-co-polypyridine, polyacrylic acid-co-methyl methacrylate, pluronics, PF68 etc).
- polymers comprise a polytelectrolyte backbone.
- copolymers which may be used in the methods according to the present invention, where the copolymers are responsive to a stimulus.
- the monomelic units are of the same type, whereas, a copolymer will usually have different types of monomelic units.
- the term "stimulus responsive polymer,” as used herein, is a polymer or copolymer which exhibits a change in a physical and/or chemical property after the addition of a stimulus.
- a typical stimulus response is a change in the polymer's solubility.
- the polymer poly(N-isopropylacrylamide) is water soluble at temperatures below about 35 °C, but become insoluble in water at temperatures of about 35 °C.
- a stimulus responsive polymer is a polyallylamine or a polyvinylamine polymer which is responsive to a multivalent ion stimulus (e.g, phosphate stimulus).
- polyelectrolyte backbone refers to a carbon containing polymer comprising two or more monomelic units, where at least 50% of the units, or at least 55% of the units, or at least 60% of the units, or at least 65% of the units, or at least 70% of the units, or at least 75% of the units, or at least 80% of the units, or at least 85% of the units, or at least 90% of the units, or at least 95% of the units, contain a charged functionality.
- at least 50% of the monomelic units include a charged group which forms a part of the unit.
- a polyelectrolyte backbone described herein contains at least two or more monomeric units, where each of the units contains a charged functionality.
- the polyelectrolyte backbone of polymers in which each of the monomeric units contains a charged functionality, such polymers may be referred to as referred to as "continuous polyelectrolytes.”
- Exemplary polyelectrolytes include, but are not limited to, polyallylamine, polyvinylamine, polyacrylic acid, polyethyleneimine, chitosan, and polyvinylphosphoric acid. It is also contemplated that one or more entities, which are different from the monomeric units, may be linked to polyelectrolyte backbone.
- hydrophobic group refers to a nonpolar entity or chemical group, which has little to no affinity for water.
- exemplary hydrophobic groups include, but are not limited to, phenyl groups, tertiary butyl groups, cyclic hydrocarbons, polycylic aliphatic hydrocarbons, polycyclic aromatic
- stimulus responsive polymers comprise a polyelectrolyte backbone having one or more hydrophobic group attached to a charged group in the backbone.
- the number of hydrophobic groups attached to the polyelectrolyte backbone is important for altering the polymer solubility, thereby to improve the stimulus responsiveness of the polymer.
- the percentage of charged groups in a polyelectrolyte backbone which are modified with a hydrophobic group is generally referred to as "percent hydrophobic modification" of the polymer. Accordingly, in various embodiments, the percent hydrophobic modification is important and ranges 1% to 85% or from 5% to 85%.
- the percent hydrophobic modification may be at least l%,or at least 2%, or at least 3%, or at least 4% or at least 5%, or at least 6%, or at least 7%, or at least 8%, or at least 9%, or at least 10%, or at least 15%, or at least 20%, or at least 25%, or at least 30%, or at least 35%, or at least 40%, or at least 45%, or at least 50%, or at least 55%, or at least 60%, or at least 65%, or at least 70%, or at least 75%, or at least 80%, or at least 85%.
- percent hydrophobic modification generally refers the ratio of unmodified polyelectrolyte charged groups to hydrophobic group modified polyelectrolyte charged groups as a percent of the total polyelectrolyte monomelic units in a polyelectrolyte polymer backbone.
- polyelectrolyte backbone further has a charged group attached to the hydrophobic group, which is distinct entity from the charged group in the backbone.
- alkyl generally refers to a straight or branched hydrocarbon chain.
- Straight chain or branched chain hydrocarbon chains refer to any substituted or unsubstituted acyclic carbon-containing compounds, including, e.g., alkanes, alkenes and alkynes.
- alkyl groups include lower alkyl, for example, methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, tert-butyl or iso-hexyl; upper alkyl, for example, n-heptyl, n-octyl, iso-octyl, nonyl, decyl, and the like; lower alkylene, for example, ethylene, propylene, propylyne, butylene, butadiene, pentene, n-hexene or iso-hexene; and upper alkylene, for example, n-heptene, n-octene, iso-octene, nonene, decene and the like.
- An ordinary skill in the art would be familiar with numerous straight, i.e., linear, as well as branched alkyl groups, which are encompassed by the present invention.
- alkyl groups may also contain various substituents in which one or more hydrogen atoms is replaced by a functional group.
- functional groups include but not limited to, carboxylic, sulfonic, phosphonic groups and the like.
- alkenyl refers to a straight or branched hydrocarbon chain, where at least one of the carbon-carbon linkages is a carbon-carbon double bond.
- aralkyr refers to an alkyl group which is terminally substituted with at least one aryl group.
- aralkenyl refers to an alkenyl group which is terminally substituted with at least one aryl group.
- aryl refers to a hydrocarbon ring bearing a system of conjugated double bonds, often comprising at least six n (pi) electrons.
- aryl groups include, but are not limited to, phenyl, naphthyl, anisyl, toluyl and xylenyl.
- fluorocarbon refers to a straight or branched carbon chain in which one or more hydrogen atoms is replaced by a fluorine group.
- Straight chain or branched chain fluorocarbon chain generally refers to any substituted or unsubstituted acyclic carbon-containing compound, including, e.g., alkanes, alkenes and alkynes.
- alkyl groups include lower alkyl, for example, methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, tert-butyl or iso-hexyl; upper alkyl, for example, n-heptyl, n-octyl, iso-octyl, nonyl, decyl, and the like; lower alkylene, for example, ethylene, propylene, propylyne, butylene, butadiene, pentene, n-hexene or iso- hexene; and upper alkylene, for example, n-heptene, n-octene, iso-octene, nonene, decene and the like.
- lower alkyl for example, methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl
- alkyl groups may also contain various substituents in which one or more hydrogen or one or more fluorine atoms is replaced by a functional group.
- functional groups include, but are not limited to, carboxylic, sulfonic, phosphonic groups and the like.
- a functional group is a group which imparts additional functionality to a polymer described herein.
- a functional group is a group, which is distinct from a hydrophobic group, and is also attached to the polyelectrolyte backbone, e.g., to a charged group in the backbone.
- a functional group can be a ligand for changing the binding properties of the polymer, for example, carboxylic acid, sulfonic acid, sulfate, primary amine, quaternary amine and diethylamino groups.
- the functional group can also alter properties or provide additional desired properties to the polymer such as, for example, altering a stimulus response or making the polymer responsive to a second stimulus.
- exemplary functional groups for altering stimulus response behavior include, but are not limited to, carboxylic acid group (pH responsive), pyridine group (pH responsive) and N-isopropylacrylamido group (temperature response).
- ligand generally refers to an entity which provides a specific binding capability for another entity.
- ligands include, but are not limited to, ion exchange groups, bioaffinity or biospecific groups, hydrophobic groups, thiophilic interaction groups, chelate or chelating groups, groups having so called pi-pi interactions with target compounds, hydrogen bonding groups, and hydrophilic groups.
- flocculation refers to the addition of a flocculant, such as a polymer described herein, to a solution in order to remove one or more suspended insoluble or soluble impurities.
- a flocculant such as a polymer described herein
- the polymer must be added to the solution at a concentration which allows for spontaneous formation of insoluble aggregates which can be removed from solution via typical solid-liquid separation methods.
- composition refers to a mixture of a target molecule or a desired product to be purified using one or more stimulus responsive polymers described herein along with one or more undesirable entities or impurities.
- the sample comprises feedstock or cell culture media into which a target molecule or a desired product is secreted.
- the sample comprises a target molecule (e.g., a therapeutic protein or an antibody) along with one or more impurities (e.g., host cell proteins, DNA, RNA, lipids, cell culture additives, cells and cellular debris).
- impurities e.g., host cell proteins, DNA, RNA, lipids, cell culture additives, cells and cellular debris.
- the sample comprises a target molecule of interest which is secreted into the cell culture media.
- a sample from which a target molecule is to be purified using one or more stimulus responsive polymers described herein is "partially purified" prior to contacting the sample with a stimulus responsive polymer. Partial purification may be accomplished, for example, by subjecting the sample to one or more purification steps, such as, e.g., one or more non-affinity chromatography steps.
- the target molecule may be separated from one or more undesirable entities or impurities either by precipitating the one or more impurities or by precipitating the target molecule.
- a stimulus responsive polymer according to the present invention binds to a biomolecule of interest which itself is a target molecule or product (e.g., a target protein or polypeptide), under a first set of conditions and precipitates the target molecule under a second set of conditions, e.g., upon the addition of a stimulus to the sample.
- a biomolecule of interest is a molecule other than a target molecule.
- the biomolecule of interest bound by a stimulus responsive polymer described herein may be a molecule which is not desired to be associated with a target molecule in a sample.
- a stimulus responsive polymer according to the present invention binds and precipitates one or more of host cell proteins, DNA, whole cells, cellular debris, viruses, endotoxins, and/or cell culture additives, upon the addition of a stimulus.
- a target molecule e.g., a target protein or polypeptide
- a polymer described herein could be purified using a polymer described herein either by precipitating the desired target molecule or by precipitating one or more undesirable entities (e.g., one or more impurities) which may be present in a sample containing the desired target molecule.
- biomolecule of interest refers to any molecule which is bound and precipitated by a stimulus responsive polymer described herein.
- the biomolecule of interest can be a desired target molecule such as, for example, a desired product or polypeptide of interest (e.g., an antibody), or it can be an undesirable entity, which needs to be removed from a sample containing the desired target molecule.
- undesirable entities include but are not limited to, for example, one or more impurities selected from host cell protein, DNA, RNA, protein aggregates, cell culture additives, viruses, endotoxins, whole cells and cellular debris.
- target molecule generally refer to a polypeptide or product of interest, which is desired to be purified or separated from one or more undesirable entities, e.g., one or more impurities, which may be present in a sample containing the polypeptide or product of interest.
- protein of interest generally refer to a therapeutic protein or polypeptide, including but not limited to, an antibody that is to be purified using a stimulus responsive polymer according to the present invention.
- polypeptide or "protein,” generally refers to peptides and proteins having more than about ten amino acids.
- a stimulus responsive polymer described herein is used to separate a protein or polypeptide from one or more undesirable entities present in a sample along with the protein or polypeptide.
- the one or more entities are one or more impurities which may be present in a sample along with the protein or polypeptide being purified.
- a stimulus responsive polymer described herein specifically binds and precipitates a protein or polypeptide of interest upon the addition of a stimulus to the sample.
- a stimulus responsive polymer described herein binds to and precipitates an entity other than the protein or polypeptide of interest such as, for example, host cell proteins, DNA, viruses, whole cells, cellular debris and cell culture additives, upon the addition of a stimulus.
- a protein or polypeptide being purified using a stimulus responsive polymer described herein is a mammalian protein, e.g., a therapeutic protein or a protein which may be used in therapy.
- exemplary proteins include, but are not limited to, for example, renin; a growth hormone, including human growth hormone and bovine growth hormone; growth hormone releasing factor;
- parathyroid hormone parathyroid hormone; thyroid stimulating hormone; lipoproteins; alpha- 1 -antitrypsin; insulin A-chain; insulin B-chain; proinsulin; follicle stimulating hormone; calcitonin; luteinizing hormone; glucagon; clotting factors such as factor VniC, factor IX, tissue factor, and von Willebrands factor; anti-clotting factors such as Protein C; atrial natriuretic factor; lung surfactant; a plasminogen activator, such as urokinase or human urine or tissue-type plasminogen activator (t-PA); bombesin; thrombin; hemopoietic growth factor; tumor necrosis factor -alpha and -beta; enkephalinase; RANTES (regulated on activation normally T-cell expressed and secreted); human macrophage inflammatory protein (MIP-1 -alpha); a serum albumin such as human serum albumin; Muellerian-inhibiting substance; relax
- a protein or polypeptide purified using a smart polymer according to the present invention is an antibody, functional fragment or variant thereof.
- a protein of interest is a recombinant protein containing an Fc region of an immunoglobulin.
- immunoglobulin refers to a protein having a basic four-polypeptide chain structure consisting of two heavy and two light chains, said chains being stabilized, for example, by interchain disulfide bonds, which has the ability to specifically bind antigen.
- single- chain immunoglobulin or “single-chain antibody” (used interchangeably herein) refers to a protein having a two-polypeptide chain structure consisting of a heavy and a light chain, said chains being stabilized, for example, by interchain peptide linkers, which has the ability to specifically bind antigen.
- domain refers to a globular region of a heavy or light chain polypeptide comprising peptide loops (e.g., comprising 3 to 4 peptide loops) stabilized, for example, by ⁇ -pleated sheet and/or intrachain disulfide bond. Domains are further referred to herein as “constant” or “variable”, based on the relative lack of sequence variation within the domains of various class members in the case of a “constant” domain, or the significant variation within the domains of various class members in the case of a “variable” domain.
- Antibody or polypeptide "domains" are often referred to interchangeably in the art as antibody or polypeptide "regions”.
- the “constant” domains of antibody light chains are referred to interchangeably as “light chain constant regions”, “light chain constant domains”, “CL” regions or “CL” domains.
- the “constant” domains of antibody heavy chains are referred to interchangeably as “heavy chain constant regions”, “heavy chain constant domains”, “CH” regions or “CH” domains.
- the “variable” domains of antibody light chains are referred to interchangeably as “light chain variable regions”, “light chain variable domains", “VL” regions or “VL” domains.
- the “variable” domains of antibody heavy chains are referred to interchangeably as “heavy chain variable regions”, “heavy chain variable domains", “VH” regions or “VH” domains.
- Immunoglobulins or antibodies may be monoclonal or polyclonal and may exist in monomelic or polymeric form, for example, IgM antibodies which exist in pentameric form and/or IgA antibodies which exist in monomelic, dimeric or multimeric form. Immunoglobulins or antibodies may also include multispecific antibodies (e.g., bispecific antibodies), and antibody fragments so long as they retain, or are modified to comprise, a ligand-specific binding domain.
- the term "fragment” refers to a part or portion of an antibody or antibody chain comprising fewer amino acid residues than an intact or complete antibody or antibody chain. Fragments can be obtained via chemical or enzymatic treatment of an intact or complete antibody or antibody chain.
- Fragments can also be obtained by recombinant means. When produced recombinantly, fragments may be expressed alone or as part of a larger protein called a fusion protein. Exemplary fragments include Fab, Fab', F(ab')2, Fc and/or Fv fragments. Exemplary fusion proteins include Fc fusion proteins.
- an immunoglobulin or antibody is directed against an immunoglobulin or antibody
- the antigen is a biologically important polypeptide and administration of the antibody to a mammal suffering from a disease or disorder can result in a therapeutic benefit in that mammal.
- antibodies directed against nonpolypeptide antigens are also contemplated.
- the antigen is a polypeptide, it may be a transmembrane molecule (e.g. receptor) or a ligand such as a growth factor.
- the term "monoclonal antibody” as used herein refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies comprising the population are identical except for possible naturally occurring mutations that may be present in minor amounts. Monoclonal antibodies are highly specific, being directed against a single antigenic site. Furthermore, in contrast to conventional (polyclonal) antibody preparations which typically include different antibodies directed against different determinants (epitopes), each monoclonal antibody is directed against a single determinant on the antigen.
- the modifier "monoclonal” indicates the character of the antibody as being obtained from a substantially homogeneous population of antibodies, and is not to be construed as requiring production of the antibody by any particular method.
- the monoclonal antibodies to be used in accordance with the present invention may be made by the hybridoma method first described by Kohler et al., Nature 256:495 (1975), or may be made by recombinant DNA methods (see, e.g., U.S. Pat. No. 4,8 6,567).
- “Monoclonal antibodies” may also be isolated from phage antibody libraries using the techniques described in Clackson et al., Nature 352:624-628 (1991) and Marks et al., J. Mol. Biol. 222:581-597 (1991), for example.
- Monoclonal antibodies may further include “chimeric” antibodies
- immunoglobulins in which a portion of the heavy and/or light chain is identical with or homologous to corresponding sequences in antibodies derived from a particular species or belonging to a particular antibody class or subclass, while the remainder of the chain(s) is identical with or homologous to corresponding sequences in antibodies derived from another species or belonging to another antibody class or subclass, as well as fragments of such antibodies, so long as they exhibit the desired biological activity (U.S. Pat. No. 4,81 ,567; and Morrison et al., Proc. Natl. Acad. Sci. USA 81 :6851-6855 (1984)).
- hypervariable region when used herein refers to the amino acid residues of an antibody which are responsible for antigen-binding.
- the hypervariable region comprises amino acid residues from a "complementarity determining region" or "CDR" (i.e. residues 24-34 (LI), 50-56 (L2) and 89-97 (L3) in the light chain variable domain and 31-35 (HI), 50-65 (H2) and 95-102 (H3) in the heavy chain variable domain; abat et al., Sequences of Proteins of Immunological Interest, 5 th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991)) and/or those residues from a "hypervariable loop" (i.e.
- Humanized forms of non-human (e.g., murine) antibodies are chimeric antibodies which contain minimal sequence derived from non-human immunoglobulin.
- humanized antibodies are human immunoglobulins (recipient antibody) in which hypervariable region residues of the recipient are replaced by hypervariable region residues from a non-human species (donor antibody) such as mouse, rat, rabbit or nonhuman primate having the desired specificity, affinity, and capacity.
- donor antibody such as mouse, rat, rabbit or nonhuman primate having the desired specificity, affinity, and capacity.
- Fv framework region (FR) residues of the human immunoglobulin are replaced by corresponding non-human residues.
- humanized antibodies may comprise residues which are not found in the recipient antibody or in the donor antibody. These modifications are made to further refine antibody performance.
- the humanized antibody will comprise substantially all of at least one, and typically two, variable domains, in which all or substantially all of the hypervariable loops correspond to those of a non-human immunoglobulin and all or substantially all of the FR regions are those of a human immunoglobulin sequence.
- the humanized antibody optionally also will comprise at least a portion of an immunoglobulin constant region (Fc), typically that of a human immunoglobulin.
- Fc immunoglobulin constant region
- an antibody which is separated or purified using a stimulus responsive polymer according to the present invention is a therapeutic antibody.
- therapeutic antibodies include, for example, trastuzumab
- HERCEPTTNTM Genentech, Inc., Carter et al (1992) Proc. Natl. Acad. Sci. USA, 89:4285-4289; U.S. Pat. No. 5,725,856
- anti-CD20 antibodies such as chimeric anti- CD20 "C2B8" U.S. Pat. No. 5,736,137
- rituximab RITUXANTM
- ocrelizumab a chimeric or humanized variant of the 2H7 antibody
- anti-VEGF antibodies including humanized and/or affinity matured anti-VEGF antibodies such as the humanized anti-VEGF antibody huA4.6.1 bevacizumab (AVASTINTM, Genentech, Inc., Kim et al (1992) Growth Factors 7:53-64, WO 96/30046, WO 98/45331); anti-PSCA antibodies (WO 01/40309); anti-CD40 antibodies, including S2C6 and humanized variants thereof (WO 00/75348); anti-CDl la (U.S.
- anti-CD4 antibodies such as the cM-7412 antibody (Choy et al (1996) Arthritis Rheum 39(l):52-56); anti-CD52 antibodies such as CAMPATH-1H
- anti-Fc receptor antibodies such as the M22 antibody directed against Fc gamma RI as in Graziano et al (1995) J. Immunol. 155(10):4996-5002; anti-carcinoembryonic antigen (CEA) antibodies such as hMN-14 (Sharkey et al (1995) Cancer Res. 55(23Suppl): 5935s-5945s; antibodies directed against breast epithelial cells including huBrE-3, hu-Mc 3 and CHL6 (Ceriani et al
- anti-CD33 antibodies such as Hu M195 (Jurcic et al (1995) Cancer Res 55(23 Suppl):5908s-5910s and CMA-676 or CDP771; anti-CD22 antibodies such as LL2 or LymphoCide (Juweid et al (1995) Cancer Res 55(23 Suppl):5899s-5907s); anti-EpCAM antibodies such as 17-1 A (PANOREXTM); anti-GpIIb/IIIa antibodies such as abciximab or c7E3 Fab (REOPROTM); anti-RSV antibodies such as MEDI-493 (SYNAGISTM); anti-CMV antibodies such as
- PROTOVIRTM anti-fflV antibodies
- PR0542 anti-hepatitis antibodies
- anti-hepatitis antibodies such as the anti-Hep B antibody OSTAVIRTM
- anti-CA 125 antibody OvaRex anti-idiotypic GD3 epitope antibody BEC2
- anti-alpha v beta3 antibody VITAXINTM anti-human renal cell carcinoma antibody such as ch-G250; ING-1; anti-human 17-1 A antibody (3622W94); anti-human colorectal tumor antibody (A33); anti-human melanoma antibody R24 directed against GD3 ganglioside; anti-human squamous-cell carcinoma (SF-25); and anti-human leukocyte antigen (HLA) antibodies such as Smart ID10 and the anti-HLA DR antibody Oncolym (Lym-1).
- HLA leukocyte antigen
- contaminant refers to any foreign or objectionable material, including a biological macromolecule such as a DNA, an RNA, one or more host cell proteins (HCPs or CHOPs), endotoxins, viruses, lipids and one or more additives which may be present in a sample containing a protein or polypeptide of interest (e.g., an antibody) being separated from one or more of the foreign or objectionable molecules using a stimulus responsive polymer according to the present invention.
- a biological macromolecule such as a DNA, an RNA, one or more host cell proteins (HCPs or CHOPs), endotoxins, viruses, lipids and one or more additives which may be present in a sample containing a protein or polypeptide of interest (e.g., an antibody) being separated from one or more of the foreign or objectionable molecules using a stimulus responsive polymer according to the present invention.
- HCPs or CHOPs host cell proteins
- endotoxins e.g., an antibody
- a stimulus responsive polymer described herein binds and precipitates a protein or polypeptide of interest from a sample containing the protein or polypeptide of interest and one or more impurities. In other embodiments, a stimulus responsive polymer described herein binds and precipitates one or more impurities, thereby to separate the polypeptide or protein of interest from one or more impurities.
- chinese hamster ovary cell protein and "CHOP,” as used interchangeably herein, refer to a mixture of host cell proteins ("HCP") derived from a Chinese hamster ovary (“CHO") cell culture.
- HCP or CHOP is generally present as an impurity in a cell culture medium or lysate (e.g., a harvested cell culture fluid containing a protein or polypeptide of interest (e.g., an antibody or immunoadhesin expressed in a CHO cell).
- a cell culture medium or lysate e.g., a harvested cell culture fluid containing a protein or polypeptide of interest (e.g., an antibody or immunoadhesin expressed in a CHO cell).
- the amount of CHOP present in a mixture comprising a protein of interest provides a measure of the degree of purity for the protein of interest.
- the amount of CHOP in a protein mixture is expressed in parts per million relative to the amount of the protein of interest in the mixture.
- HCP refers to the proteins, other than target protein, found in a lysate of the host cell.
- cell culture additive refers to a molecule
- a stimulus responsive polymer binds and precipitates one or more cell culture additives.
- Exemplary cell culture additives include anti-foam agents, antibiotics, dyes and nutrients.
- CHOP ppm (CHOP ng ml)/(protein of interest mg ml).
- ppm refers to (CHOP ng)/(protein of interest mg)).
- the terms "isolating,” “purifying” and “separating,” are used interchangeably herein, in the context of purifying a target molecule (e.g., a polypeptide or protein of interest) from a composition or sample comprising the target molecule and one or more impurities, using a stimulus responsive polymer described herein.
- a target molecule e.g., a polypeptide or protein of interest
- the degree of purity of the target molecule in a sample is increased by removing (completely or partially) one or more impurities from the sample by using a stimulus responsive polymer, as described herein,.
- the degree of purity of the target molecule in a sample is increased by precipitating the target molecule away from one or more impurities in the sample.
- a purification process additionally employs one or more "chromatography steps.” Typically, these steps may be carried out, if necessary, after the separation of a target molecule from one or more undesired entities using a stimulus responsive polymer according to the present invention.
- a "purification step" to isolate, separate or purify a polypeptide or protein of interest using a stimulus responsive polymer described herein may be part of an overall purification process resulting in a
- composition or sample which term is used herein to refer to a composition or sample comprising less than 100 ppm HCP in a composition comprising the protein of interest, alternatively less than 90 ppm, less than 80 ppm, less than 70 ppm, less than 60 ppm, less than 50 ppm, less than 40 ppm, less than 30 ppm, less than 20 ppm, less than 10 ppm, less than 5 ppm, or less than 3 ppm of HCP.
- the term "clarification step,” as used herein, generally refers to one or more initial steps in the purification of biomolecules.
- the clarification step generally comprises removal of cells and/or cellular debris using one or more steps including any of the following alone or various combinations thereof, e.g., centrifugation and depth filtration, precipitation, flocculation and settling.
- Clarification step generally involves the removal of one or more undesirable entities and is typically performed prior to a step involving capture of the desired target molecule.
- Another key aspect of clarification is the removal of soluble and insoluble components in a sample which may later on result in the fouling of a sterile filter in a purification process, thereby making the overall purification process more economical.
- the present invention provides an improvement over the conventional clarification steps commonly used in various purification schemes, as demonstrated by reduced turbidity/impurities and higher throughput of downstream filters, described in the Examples herein.
- chromatography refers to any kind of technique which separates an analyte of interest (e.g., a target molecule) from other molecules present in a mixture. Usually, the analyte of interest is separated from other molecules as a result of differences in rates at which the individual molecules of the mixture migrate through a stationary medium under the influence of a moving phase, or in bind and elute processes.
- chromatography resin or “chromatography media” are used interchangeably herein and refer to any kind of phase (e.g., a solid phase) which separates an analyte of interest (e.g., a target molecule) from other molecules present in a mixture.
- the analyte of interest is separated from other molecules as a result of differences in rates at which the individual molecules of the mixture migrate through a stationary solid phase under the influence of a moving phase, or in bind and elute processes.
- chromatography media include, for example, cation exchange resins, affinity resins, anion exchange resins, anion exchange membranes, hydrophobic interaction resins and ion exchange monoliths.
- capture step generally refers to a method used for binding a target molecule with a stimulus responsive polymer or a
- the target molecule is
- the capture step can be conducted using a chromatography media, such as a resin, membrane or monolith, or a polymer, such as a stimulus responsive polymer, polyelectolyte or polymer which binds the target molecule.
- a chromatography media such as a resin, membrane or monolith
- a polymer such as a stimulus responsive polymer, polyelectolyte or polymer which binds the target molecule.
- salt refers to a compound formed by the interaction of an acid and a base.
- Various salts which may be used in various buffers employed in the methods described herein include, but are not limited to, acetate (e.g. sodium acetate), citrate (e.g., sodium citrate), chloride (e.g., sodium chloride), sulphate (e.g., sodium sulphate), or a potassium salt.
- acetate e.g. sodium acetate
- citrate e.g., sodium citrate
- chloride e.g., sodium chloride
- sulphate e.g., sodium sulphate
- a multivalent salt is used as a stimulus which results in changing the solubility of a polymer responsive to the salt stimulus, usually resulting in precipitating the polymer out of solution.
- exemplary multivalent salts which may be used include, for example, phosphate and sulfate.
- a multivalent salt when used a stimulus as described herein, may be added as a stand alone reagent to a sample containing a biomolecule of interest along with a stimulus responsive polymer.
- the salt may be attached to a substrate, e.g., a membrane.
- a membrane is modified with polyvinylphosphate, which is a multivalent salt containing polymer coating.
- a multivalent ion used as a stimulus, as described herein, is considered less detrimental to protein structure and stability, relative to other stimuli such as temperature and pH.
- multivalent salts are capable of interacting with one or more entities, thereby to form an associated species or complex. Accordingly, such salts may also be referred to as "complex forming salts.”
- complex forming salts and their resultant complexes are multivalent cations such as Cu 2+ and Ca 2+ and their complexes with the carboxylic acid groups found in ethylenediaminetetraacetate; multivalent anions such as phosphate (PC 3- ) and citrate and their complexes with primary amines found in polyallylamine; and ion-associating salts such as perchlorate, dodecyl sulfate and dodecyl benzene sulfonate and their complexes with primary amines found in polyallylamine.
- an "ion-associating salt” is a univalent (cation or anion), bulky and charge-dispersed salt.
- an ion-associating salt is used as a stimulus which results in changing the solubility of a polymer responsive to the salt stimulus, thereby resulting in precipitating the polymer out of solution.
- Exemplary ion- associating salts which may be used include, for example, perchlorate, dodecyl sulfate, dodecyl benzene sulfonate, tetraphenyl borate and hexanitrodiphenol amine.
- solvent generally refers to a liquid substance capable of dissolving or dispersing one or more other substances to provide a solution.
- Solvents include aqueous and organic solvents, where useful organic solvents include a non-polar solvent, ethanol, methanol, isopropanol, acetonitrile, hexylene glycol, propylene glycol, and 2,2-thiodiglycol.
- pi or "isoelectric point" of a polypeptide, as used interchangeably herein, refers to the pH at which the polypeptide's positive charge balances its negative charge, pi can be calculated from the net charge of the amino acid residues or sialic acid residues of attached carbohydrates of the polypeptide or can be determined by isoelectric focusing.
- IgG was spiked to a concentration of 1.3 g L.
- the level of host cell proteins (HCP) was found to be 8300 ng mL using an ELISA (Cygnus # CM015).
- the pH of the unclarified cell culture was pH 7.2.
- Example 2 Synthesis of a stimulus responsive polymer comprising a
- a stimulus responsive polymer comprising a polyallylamine (BzMPAA) backbone modified with a hydrophobic group was synthesized.
- a polymer comprising a cationic polyelectrolyte backbone modified with a hydrophobic group was synthesized using a mixture of 10.3 g of 40% wt linear polyallylamine (NITTOBO, 150 kD), 2 g of lithium hydroxide and 20 mL of 50% water/methanol, which was stirred until well mixed.
- a solution of 2.1 mL of benzyl chloride in 15 mL of methanol was added to the polymer solution. The resulting mixture was heated at 60 °C for 14 hours.
- the benzyl modified polyallylamine was precipitated at the end of the reaction period as a result of thermodynamic
- Figure 1 depicts a schematic of the reaction of the polyallylamine polyelectrolye polymer with a hydrophobic group, i.e., benzylchloride.
- a 10% solution of BzMPAA was prepared by dissolving 1 Og of the polymer from Example 2 in 90g of 1M acetic acid with continuous agitation at room temperature for 16 hours. The resulting viscous solution was slightly hazy.
- BzMPAA in clarification of a non-expressing cell culture fluid rccFi
- BzMPAA from Example 3 was added in amounts of 0.2g, 0.3g, 0.4g and 0.5g to a 5mL sample of the unclarified cell culture fluid (CCF) from Example 1.
- the samples were mixed at room temperature for 2 minutes. Since polymer addition reduced the pH to a range of pH 4.5 to 5.5, the pH of the mixtures were adjusted using 2M Tris base to a pH of 7.
- 0.043g of potassium phosphate dibasic was added in order to precipitate the polymer-target molecule, cell and cell debris complex.
- the precipitate, in the form of a dispersed solid suspension was mixed continuously for five minutes.
- the precipitate was then collected via centrifugation (4000 rpm for 1 minute).
- the supernatant from each sample was then filtered through a 0.2um Durapore® filter.
- the resulting purification is detailed in Table 1 below.
- BzMPAA from Example 3 was added to four samples (0.4g each) containing 5mL of the unclarified cell culture fluid from Example 1. The samples were mixed at room temperature for 2 minutes. Since polymer addition reduced the pH to a range of pH 4.5 to 5.5, the pH of the mixtures were adjusted using 2M Tris base to pHs of 5.5, 6.5 7.5 and 8.5, respectively. To the resulting solution, 0.043g of potassium phosphate dibasic was added in order to precipitate the polymer-target molecule, cell and cell debris complex. The precipitate, which was in the form of a dispersed solid suspension, was mixed continuously for five minutes. The precipitate was then collected via centrifugation (4000 rpm for 1 minute). The supernatant from each sample was then filtered through a 0.2um Durapore® filter. The resulting purification is detailed in Table 1 below, which describes BzMPAA purification of spiked non- expressing CHO CCF.
- Example 6 Assaying for the purity levels resulting from the use of BzMPAA in
- Example 4 and Example 5 were assayed for IgG recovery using an affinity Protein A analytical HPLC assay.
- the level of IgG in solution was alternatively measured using an analytical Protein A column. Specifically, a Poros A/20 Protein A column (Applied Biosystems) was equilibrated with PBS, eluted with 0.1M lysine (pH 2) and cleaned with 6M guanidine HCl.
- An IgG standard curve was created using a series of varying injection volumes of polyclonal IgG (Seracare). Samples were injected and IgG concentrations determined from the standard curve.
- Example 4 and Example 5 were also assayed for DNA using a standard pico green assay and Herring sperm DNA as a standard.
- DG44 DG44 cell line
- MAb Monoclonal antibody
- HCP host cell proteins
- the solution was then diluted with H 2 O to a final volume of 400mL (1% polymer solution), potassium dibasic phosphate (K 2 HPO4) (3.48g) is added under stirring and pH of the solution was adjusted to pH 6.8 to precipitate the modified polymer.
- K 2 HPO4 potassium dibasic phosphate
- PAA polyallylamine
- NITTOBO 150 kD; 40% wt/wt.
- Benzyl chloride 3.23g, 2.94mL was then added and stirred for few minutes at room temperature and subsequently heated to 60°C overnight for 17 hours. The reaction was then cooled to room temperature and solvent was removed. The precipitated polymer was washed with water then stirred in 1M aqueous AcOH solution (40mL) until complete solubilization is achieved.
- the solution was then diluted with H 2 O to a final volume of 400mL (1% polymer solution), potassium dibasic phosphate (K 2 HPO4) (3.48g) is added under stirring and pH of the solution was adjusted to pH 6.8 to precipitate the modified polymer.
- K 2 HPO4 potassium dibasic phosphate
- Example 11 Synthesis of diphenyl modified polvallylamine (DPhMPAA) [00133]
- DPhMPAA diphenyl modified polvallylamine
- the chloro-diphenyl methane (3.68g, 3.23mL) was subsequently added, stirred for few minutes at room temperature then heated to 60°C overnight for 17 hours. The reaction was subsequently allowed to cool to room temperature and the solvent was removed. The precipitated polymer was washed with water and stirred in 1M aqueous AcOH solution (40mL).
- polymer was filtered out, washed with H 2 O and dried in a vacuum oven overnight at 60°C.
- the polymer was collected by filtration over a fritted funnel and finally dried overnight in a vacuum oven overnight at 50-60°C.
- 3,4-dichlorobenzyl chloride (0.57g, 0.40mL) was subsequently added, stirred for few minutes at room temperature and then heated to 60°C overnight for 21 hours.
- the polymer was collected by filtration over a fritted funnel and finally dried overnight in a vacuum oven at 50-60°C.
- phenyl-ben2yl modified polyallylamine was synthesized as follows. 4.7g of polyallylamine (PAA) (Nittobo, 150 kD; 40% wt./wt.) was placed in a 100 mL round bottom flask and a solution of 3.34g of sodium hydroxide (1.2 Eq. per monomer) in 40mL 50/50 H 2 O/1,2 dimethoxyethane (DME) was added at room temperature under magnetic stirring and in small amounts. 4- phenylbenzyl chloride (lg) was then added and the mixture heated overnight at 55°C for 20 hours. The reaction was subsequently allowed to cool to room temperature,
- PAA polyallylamine
- DME dimethoxyethane
- phenyl-benzyl modified polyallylamine was synthesized as follows. 2.8g polyallylamine (PAA) (Nittobo, 150 kD; 40% wt./wt.) was placed in a 100 mL round bottom flask and a solution of 3.34g of sodium hydroxide (1.2 Eq. per monomer) in 24mL 50/50 H 2 O/1,2 dimethoxyethane (DME) was added at room temperature under magnetic stirring and in small amounts. 4- phenylbenzyl chloride (lg) was subsequently added and the mixture was heated at 55°C overnight for 20 hours.
- PAA polyallylamine
- DME dimethoxyethane
- the reaction was subsequently allowed to cool to room temperature, DME was removed under vacuum at 60-70°C and the remaining solvent was removed from the precipitated polymer. This latter was washed with water then stirred in 1M aqueous AcOH solution (32mL) and stirred overnight to complete the solubilization.
- the solution was subsequently diluted with H 2 O to a final volume of 200mL (1% polymer solution), potassium dibasic phosphate (K2HPO 4 ) (1.74g) was added under stirring and pH of the solution was adjusted to pH 7.0 to precipitate the purified polymer.
- the polymer was collected by filtration over a fritted funnel and finally dried overnight in a vacuum at 50-60°C.
- stimulus responsive polymers described above in Examples 8-16, were evaluated for clarification of CCF, preparation of which is described above in Example 7.
- a polymer solution from Examples 8-16 was added in amounts of 0.2g, 0.3g, 0.4g and 0.5g to a 5mL sample of the unclarified cell culture fluid. The samples were mixed at room temperature for 2 minutes. Since polymer addition reduced the pH to a range of pH 4.5 to 5.5, the pH of the mixtures were adjusted using 2M Tris base to a pH of 7. To the resulting solution, 0.043g of potassium phosphate dibasic was added in order to precipitate the polymer-target molecule, cell and cell debris complex.
- the precipitate in the form of a dispersed solid suspension, was mixed continuously for five minutes. The precipitate was then collected via centrifugation (4000 rpm for 1 minute). The supernatant from each sample was subsequently filtered through a 0.2 ⁇ Durapore® filter. The resulting purification is detailed in Table 2.
- Example 18 Clarification of CCF with polymers at different PH
- Example 19 Clarification of CCF using polymers responsive to different levels of multivalent ion stimulus
- polymers described in Examples 8-15 were evaluated for the clarification of CCF, using different amounts of multivalent ion stimulus. Specifically, polymers from Examples 8-15 were added to four samples (0.4g each) containing 5mL of the unclarified cell culture fluid, described in Example 7. The samples were mixed at room temperature for 2 minutes. Since polymer addition reduced the pH to a range of pH 4.5 to 5.5, the pH of the mixtures were adjusted using 2M Tris base to pHs of 5.5, 6.5 7.5 and 8.5, respectively.
- Example 20 Clarification of CCF using a commonly used flocculant
- polymers described in Examples 8-16 were assayed for IgG recovery using an affinity Protein A analytical HPLC assay.
- the level of IgG in solution was alternatively measured using an analytical Protein A column.
- a Poros A/20 Protein A column (Applied Biosystems) was equilibrated with PBS, eluted with 0.1M lysine (pH 2) and cleaned with 6M guanidine HCl.
- An IgG standard curve was created using a series of varying injection volumes of polyclonal IgG (Seracare). Samples were injected and IgG concentrations determined from the standard curve.
- Samples from Examples 8-16 were assayed host cell proteins (HCP) using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Cygnus Technologies Inc., Southport, N.C., Cygnus # 3G). Samples from Examples 8-16 were assayed for DNA using a standard pico green assay and Herring sperm DNA as a standard. To assess the reduction of cells and cell debris, the turbidity was measured after centrifugation for 1 minute at 4000 rpm.
- HCP host cell proteins
- ELISA enzyme-linked immunosorbent assay
- a benzyl modified polyethyleneimine stimulus responsive polymer was synthesized as follows. lOg PEI (Aldrich, 750 kD; 50% wt./wt.) is placed in a 100 mL round bottom flask and a solution of ⁇ 3.34g of sodium hydroxide (1.2 Eq. per monomer) in 25mL H 2 O is added at room temperature under magnetic stirring and in small amounts. Benzyl chloride (2.30g, 2.09mL) is then added, stirred for few minutes at room temperature and then heated overnight at 60°C for 17 hours. The reaction is then allowed to cool to room temperature and the solvent is removed.
- PEI Aldrich, 750 kD; 50% wt./wt.
- the precipitated polymer is washed with water then stirred in 1 M aqueous AcOH solution (40mL) until complete solubilization is achieved.
- the solution is subsequently diluted with H 2 O to a final volume of 400mL (1% polymer solution), potassium dibasic phosphate (K 2 HPO4) (3.48g) is added under stirring and pH of the solution is adjusted to pH 6.8 to precipitate the modified polymer.
- K 2 HPO4 potassium dibasic phosphate
- a benzyl modified polyvinylamine was synthesized as follows. 32g of Poly(vinylamine) (PVA) hydrochloride (MW 83,500,
- Example 24 Comparison of modified and unmodified polymer in clarification in presence of stimulus [00146]
- Polyallyamine (PAA, Nittobo, 150 kD; 40% wt./wt.) and the polymer from Example 10 (Bz-MPAA) were added to an aqueous solution to create a final polymer concentration of 0.2%wt and 0.4%, respectively.
- Potassium hydrogen phosphate was used as a stimulus and was added to the polymer solutions of PAA and Example 10 in varying amounts and the turbidity and nature of the aggregates formed was recorded. The results are shown in Table 3 below.
- Example 25 Comparison of modified and unmodified polymer in clarification in presence of a stimulus
- Unmodified polymers polyallyamine (PAA, Nittobo, 150 kD; 40% wt/wt.), polyethyleneimine (PEI, Aldrich, 750 kD; 50% w /wt.), polyvinyl amine (Poly(vinylamine) (PVA) hydrochloride, MW 83,500, Air Products and Chemicals Inc.) and the modified polymers from Examples 10 (Bz-MPAA), Example 22 (BzMPEI) and Example 23 (BzMPVA) were added to an aqueous solution to create a final polymer concentration of 0.2% and 0.4% by weight, respectively. Potassium hydrogen phosphate (150mM) was used as a stimulus and was added to each of 0.4% polymer solutions and the turbidity and nature of the aggregates formed was recorded. The results are shown in Table 4.
- Example 28 Clarification of CCF using the polymer HC-t-BuMPAA at different polymer concentrations
- unclarified CCF was clarified using a polymer according to the present invention as follows. 178, 356, or 534 ⁇ of HC-t- BuMPAA from Example 26 was added to 5ml of un-clarified cell culture fluid from Example 1 containing 25 mM sodium phosphate and adjusted to pH 8.7 using 25 ⁇ of 3Macetic acid. After the addition of the polymer, final pH of the solution was titrated to 7.2 using 3M acetic acid, thereby precipitating the polymer-cells complex.
- the precipitate in the form of a dispersed solid suspension, was mixed continuously for another 5 min. The precipitate was then collected by centrifugation (4000 rpm for 1 min) and the supernatant was filtered through a 0.2 ⁇ Durapore® filter. Regardless of the concentration of the polymer used, the process resulted in 100% Mab recovery.
- a stimulus responsive polymer comprised of repeat units containing primary amines was synthesized from a monomer as follows. 165 g of deionized water and 22.5 g of N-vinylformamide (NVF) (SIGMA-ALDRICH, 98%) was placed in a 250 niL round bottom flask. The flask was equipped with a magnetic stirrer and a N 2 dipstick. The solution was stirred and purged with N 2 for 0.5 hours followed by heating to 45 °C over an additional 0.5 hours with continuous purging.
- NVF N-vinylformamide
- Initiator solution was prepared by adding 0.288g of 2,2' Azobis(2-amidinopropane)dihydrochloride (ABAP) (Aldrich) to 10 mL of deionized water and dissolved. To the 250 mL round bottom flask the initiator solution was added under N 2 atmosphere. The solution was heated at 55 °C for 1 hour, followed by heating at 65 °C for 2 hours, further followed by heating at 75 °C for 1 hour with vigorous stirring under nitrogen. A viscous, homogenous solution was obtained and allowed to cool to room temperature. The viscosity was determined by Brookfield Viscosity DV-II+ Pro Visocometer (setting 1 0 RP , 45% torque, spindle #34).
- ABSP 2,2' Azobis(2-amidinopropane)dihydrochloride
- the viscosity of the resulting solution was 278-350 centipoise (cP).
- the solution was transferred to a 500 mL flask and diluted with 330 mL of H 2 0 and 40 g of 50% NaOH was added with stirring. The solution was heated at 85 °C for 8 hours.
- a small sample was tested for sensitivity to a phosphate stimulus by adding a drop of 2 molar sodium phosphate to the hydrolyzed polymer and observing a white solid precipitate from the solution upon addition of the phosphate ion.
- 25% HCl was added drop wise, until a pH of around 2 was reached.
- the solution was stirred vigorously overnight and a homogenous yellow solution was obtained.
- 100 mL of 4 molar NaOH was added with stirring along with 500 mL isopropyl alcohol.
- the polymer was isolated by addition of with deionized water. The solid polymer was dried over night in a vacuum oven at 65 °C.
- the partially dry polymer was frozen with liquid nitrogen and ground to a fine powder and further dried in a vacuum oven at 65 °C for 24 hours. Finally, 40.7 g of a dry powder was recovered and dissolved in 1 molar acetic acid to a final concentration of 5% w/w.
- the resulting polymers were each isolated from the individual reaction solutions by bringing the volume of the reaction solution to 500 mL with deionized water, adjusting the pH to 8 with 25% HCl, and adding 100 g of 2 molar sodium phosphate. Upon addition of the phosphate ion, a solid precipitate was collected via vacuum filtration. The solid from each reaction was individually washed with deionized water and dissolved into 300 mL of 1 molar acetic acid.
- the polymer was further purified by adjusting the individual solutions to pH 7.4, precipitating the polymer with drop wise addition of 2 molar sodium phosphate, filtering the resulting solids, washing the solids with water, followed isopropyl alcohol, and drying in a vacuum oven at 65 °C for 2 days.
- Each sample of dried polymer was frozen with liquid nitrogen and ground to a fine powder.
- the mass of dried polymer recovered was 1.44, 2.12, and 2.47 g for jars 1 , 2, and 3, respectively.
- the individual polymers were each dissolved to make a 2% solution in 1 molar acetic acid.
- Example 31 Deprotection of amines via hydrolysis of a very high molecular weight
- Very high molecular weight polymer generally refers to a polymer having a molecular weight equal or greater than lOOOKDa.
- a stimuli responsive polymer comprised of repeat units containing primary amines was prepared as follows.
- a 2 liter glass jar 40 g of poly(N- vinylacetamide)-linear homopolymer (POLYSCIENCES, INC.) with an average molecular weight of 4,060 kDa was dissolved into 0.8 liter of deionized water by vigorous stirring for 16 hours.
- 140 g of concentrated HCl was added with continuous stirring over 1 hour.
- the jar was lightly capped and the solution was heated to 99 °C for 5 days with intermittent rotation to mix the solution. After 5 days of heating, the solution was allowed to cool to room temperature and the total volume was adjusted to 4 liters with deionized water.
- the solution was adjusted to pH 7 with 8 molar NaOH with vigorous stirring.
- the hydrolyzed product was precipitated with drop wise addition of 2 molar sodium phosphate until no further precipitate was observed.
- the white precipitate was washed with deionized water and pressed to remove excess water.
- the recovered polymer was dried in a vacuum oven at 65 °C for 2 days. The dried polymer was frozen with liquid nitrogen and ground to a fine powder. The recovered dry mass was 42.5 g.
- a 2% solution was made by dissolving the dried powder into 1 molar acetic acid and 0.08% HCl.
- the resulting solution was compared to a 2% solution of starting material (poly(N-vinylacetamide)-linear homopolymer) for response to a phosphate or citrate stimuli. This was performed by drop wise addition of 2 molar sodium phosphate or 0.2 molar sodium citrate to 50 mL samples of both the starting material and the resulting hydrolyzed polymer.
- Figure 5 depicts a process for deprotection of polyamine polymer, thereby resulting in the formation of a stimulus responsive Polyvinylamine (PVA).
- PVA Polyvinylamine
- Example 32 Synthesis of a hvdrophobicallv modified polyvinylamine (PVA) stimuli responsive polymer based on a deprotected polvfN- vinylacetamide)
- the resulting solids were washed with deionized water, followed by isopropyl alcohol, and were dried in a vacuum oven at 65 °C for 2 days.
- the dry solids were ground to a fine powder and the final dry mass of polymer collected was 1.44 g.
- a 2% solution was made by dissolving the dried powder into 1 molar acetic acid and 0.08% HCl.
- the resulting solution was tested for sensitivity to a multivalent ion stimulus by drop wise addition of 2 molar sodium phosphate or 0.2 molar sodium citrate to 5mL samples of 0.5% polymer solutions. Upon addition of the phosphate or citrate ions a white precipitate is observed, thereby indicating that the polymer was responsive to a multivalent anion stimulus.
- Figure 6 provides a schematic of the synthesis process described in this
- Example 33 Synthesis of a stimulus responsive vinylamine / vinylbutylether copolymer (VA-co-VBE)
- a stimulus responsive polymer comprised of repeat units containing primary amines and butylether was synthesized from monomers as follows. 90 g of octane, 2.5 g of Span-85 (SIGMA), 16 g of N-vinylformamide (NVF)(ALDRICH,
- Initiator solution was prepared by adding 0.10 g of 2,2' Azobis(2- amidinopropane)dihydrochloride (ABAP) (ALDRICH) to 10 mL of deionized water and dissolved. The initiator solution was charged to the 250 mL round bottom flask containing the reaction solution under nitrogen purged atmosphere.
- ABAP 2,2' Azobis(2- amidinopropane)dihydrochloride
- the solution was heated at 55 °C for 1 hour, followed by heating at 60 °C for 1 hour, further followed by heating at 70 °C for 1 hour and followed by heating at 80 °C for 1 hour, with vigorous stirring with continuous nitrogen purging. This resulted in a two phase solution with a viscous gel layer on the bottom. The top layer was decanted and discarded. To the bottom layer, 200 mL of deionized water was added and 20 g of 50% NaOH was added with vigorous stirring. The solution was heated at 80 °C for 6 hours. After 6 hours, the solution was removed from heat and allowed to cool to room temperature. The volume was increased to 2 L with deionized water.
- the product was isolated by dropwise addition of 2 molar sodium phosphate, resulting in a large white precipitate.
- the precipitate was collected by decanting the supernatant and the precipitate was washed with deionized water.
- the isolated polymer was dissolved in 500 mL deionized water, 10 g acetic acid, and 2 g concentrated HCl.
- the resulting solution was tested for sensitivity to a multivalent ion stimulus by drop wise addition of 2 molar sodium phosphate or 0.2 molar sodium citrate to 5mL samples of 0.5% polymer solutions. Upon addition of the phosphate or citrate ions a white precipitate was observed, thereby indicating that the polymer was responsive to a multivalent anion stimulus.
- Figure 7 depicts a schematic of the reaction described in this Example.
- Example 34 Synthesis of very high molecular weight polwinylamine fPVA) stimulus responsive polymer from NVF monomer via inverse emulsion
- a stimulus responsive polymer comprised of repeat units containing primary amines was synthesized from a monomer as follows. 90 g of octane, 2.5 g of Span-85 (SIGMA), 16 g of N-vinylformamide (NVF)(ALDRICH, 98%), and 30 g of deionized water was placed in a 250 mL round bottom flask. The flask was equipped with a magnetic stirrer and a N 2 dipstick. The solution was stirred and purged with N 2 for 1 hour as the temperature was increased to 55°C.
- SIGMA Span-85
- NVF N-vinylformamide
- Initiator solution was prepared by adding 0.20 g of 2,2' Azobis(2-amidinopropane)dihydrochloride (ABAP) (ALDRICH) to 20 mL of deionized water and dissolving.
- the initiator solution was charged to the 250 mL round bottom flask containing the reaction solution under nitrogen purged atmosphere. The solution was heated to 60 °C for 2 hours, followed by heating to 75 °C for 1 hour with vigorous stirring with continuous nitrogen purging. A two phase solution results with a viscose gel layer on the bottom. The top layer is decanted and discarded. To the bottom layer 500 mL of deionized water is added and 48 g of 50% NaOH is added with vigorous stirring.
- the solution was heated to 80 °C for 16 hours. After 16 hours the solution was removed from heat and allowed to cool to room temperature. The volume was increased to 1 L with deionized water.
- the product was isolated by drop wise addition of 2 molar sodium phosphate resulting in a large white precipitate. The precipitate was collected by decanting the supernatant and the precipitate was washed with deionized water and soaked in isopropyl alcohol for 2 hours and finally washed again with deionized water.
- the resulting solid mass was dried in a vacuum oven at 65 °C for 3 days. The dried polymer was frozen with liquid nitrogen and ground to a fine powder and further dried for 1 day. The resulting mass of the dry powder was 21.5 g.
- a 2% solution was made by dissolving the dried powder into 1 molar acetic acid and 0.08% HCl. The resulting solution was tested for sensitivity to a multivalent ion stimulus by drop wise addition of 2 molar sodium phosphate or 0.2 molar sodium citrate to 50mL samples of 1% polymer solutions. Upon addition of the phosphate or citrate ions a white precipitate is observed.
- Example 35 Synthesis of a high molecular weight hydrophobically modified
- PVA polyvinylamine
- MW 340 kDa, 20 % solids, pH 7-9) obtained from BASF, a hydrophobically modified stimulus responsive polymer was produced as follows. 300 g of Lupamin 9095 (approximately 60 g of polyvinylamine) was added to a 2 liter glass jar. Next, 40 g of NaOH pellets and 500 mL of deionized water were dissolved and added to the jar. This was followed by the addition of 500 mL 1,2-dimethoxyethane (SIGMA) as a co- solvent and the solution was stirred vigorously until homogenous. Next, 17.66 g benzyl chloride (ACROS ORGANICS, 99%) was added to the reaction jar with stirring.
- SIGMA 1,2-dimethoxyethane
- the solution was heated to 60 °C for 16 hours with magnetic stirring. The solution was subsequently allowed to cool to room temperature and transferred to a 5 liter beaker. Next, 1500 mL of deionized water was added with stirring. The pH was adjusted to 5 with glacial acetic acid. The product was precipitated with slow addition of 250 mL 2 molar sodium phosphate and the solid was collected and washed with deionized water.
- the polymer was further purified by the following method.
- the solid was dissolved in 2 liter of 1 molar acetic acid with stirring. The total volume was brought to 10 liters with deionized water and the pH is adjusted to 7 with drop wise addition of 50% NaOH.
- the product was precipitated with addition 600 g of 2 molar sodium phosphate.
- the solid was isolated via vacuum filtration and washed with deionized water. The resulting solid mass was dried in a vacuum oven at 65 °C for 3 days. The dried polymer was frozen with liquid nitrogen and ground to a fine powder and further dried for 1 day. The resulting mass of the dry powder was 46 g.
- Example 36 200 g scale synthesis of a hydrophobically modified polyallylymine based stimulus responsive polymer
- the solid was dissolved in 3 liter of 1 molar acetic acid with stirring. The total volume was brought to 10 liters with deionized water and the pH was adjusted to 7 with drop wise addition of 50% NaOH. The product was precipitated with addition 800 g of 2 molar sodium phosphate and the solids was collected and washed with deionized water. The resulting solid mass was dried in a vacuum oven at 65 °C for 3 days. The dried polymer was frozen with liquid nitrogen and ground to a fine powder and further dried for 1 day. The resulting mass of the dry powder was 250 g. A small sample was dissolved in 1 molar acetic acid with stirring. The total volume was brought to 10 liters with deionized water and the pH was adjusted to 7 with drop wise addition of 50% NaOH. The product was precipitated with addition 800 g of 2 molar sodium phosphate and the solids was collected and washed with deionized water. The resulting solid mass was dried in a vacuum oven at 65 °C for 3 days. The
- Example 37 Determination of flocculation performance and supernatant quality for increasing doses of a stimulus responsive polymer vs a cationic polvelectrolvte in a CHO cell culture
- the stimulus responsive polymers according to the present invention were compared to a known polymer, i.e., chitosan, for certain desirable properties.
- CHO cell culture was prepared using a method, as described in
- Example 1 A solution of 2% w/w solution of medium molecular weight chitoson (MMW chitosan) (Sigma-Aldrich) was prepared in 1 molar acetic acid. A 2% w/w solution of hydrophobically modified polyallylamine based stimulus responsive (33%- BnPAA) polymer was prepared according to Example # 36. 10 mL of the CHO cell culture was dispensed into 15 mL corneal tubes. Individual polymer doses of 0.0, 0.1 , 0.2, 0.03, 0.04, 0.05, 0.06, 0.07.
- MMW chitosan medium molecular weight chitoson
- 33%- BnPAA hydrophobically modified polyallylamine based stimulus responsive
- the stimulus responsive polymer continues to be an effective flocculant/precipitant regardless of increase in the dose.
- Example 38 Determination of flocculation performance and supernatant quality with increasing doses of a stimulus responsive polymer in the presence
- CHO cell culture was prepared using a method, as described in
- Example 1 A 2% w/w solution of hydrophobically modified polyallylamine based stimuli responsive (33%-BnPAA) polymer was prepared according to Example # 36. Individual polymer doses of 0.0, 0.05, 0.06, 0.07. 0.08, 0.09, 0.1, 0.14, 0.18, 0.22, and 0.4 % w/v were added to each conical tube containing CHO cell culture in triplicates (one for phosphate and one for citrate and one for no stimuli) for 33%-BnPAA polymer. The pH was adjusted to 7.2 and a stimulus of 150 mM sodium phosphate or 150 mM sodium citrate was applied or no stimulus was applied. All the conical tubes were centrifuged at 3000 RPM for 2 minutes and the supernatant was decanted and the centrate turbidity was determined. The results from the experiments described in this example are shown in Table 6 and Figure 11.
- Table 6 and Figure 11 summarize the results of a representative experiment to demonstrate that a stimulus responsive polymer (e.g., described in Example 36) can operate as a non-stimulus responsive flocculant (similar to Chitosan data in Example 37), requiring polymer dose optimization without a stimulus.
- a stimulus responsive polymer e.g., described in Example 36
- a non-stimulus responsive flocculant similar to Chitosan data in Example 37
- the stimulus responsive polymer does not require dose optimization as the centrate turbidity is not effected by increasing polymer dose.
- Example 39 Determination of flocculation performance and supernatant quality with increasing doses of an unmodified stimulus responsive polymer in
- CHO cell culture was prepared using a method, as described in
- Example 1 A 2% w/w solution of unmodified polyallylamine based stimuli responsive polymer was prepared by dissolving Polyallyamine (PAA, NITTOBO, 150 kD; 40% wt./wt.) in 1 molar acetic acid. 10 mL of the CHO cell culture is dispensed into 15 mL conical tubes. Individual polymer doses of 0.0, 0.05, 0.06, 0.07. 0.08, 0.09, 0.1, 0.14, 0.18, 0.22, and 0.4 % w/w was added to each conical tube containing CHO cell culture. The pH was adjusted to 7.2 and a stimulus of 150 mM sodium phosphate was applied. All the conical tubes were centrifuged at 3000 RPM for 2 minutes and the supernatant was decanted and the turbidity was determined. The results from the experiments described in this example are shown in Table 7.
- Example 40 Determination of flocculation performance, settling time, and
- CHO cell culture was prepared using a method as described in Example
- a 10% w/w solution of a hydrophobically modified polyallylamine based stimuli responsive (33%-BnPAA) polymer was prepared similar to Example # 36. 50 mL of CHO cell culture was placed in a 100 mL glass graduated cylinder in duplicates. In one of the cylinders, the stimulus responsive polymer was added to a dose of 0.5%, the pH was adjusted to 7 with drop wise addition of 2 molar tris base, the sodium phosphate concentration was adjusted to 50 mM by addition of a 2 molar sodium phosphate solution, and the solution was stirred for 2 minutes.
- a hydrophobically modified polyallylamine based stimuli responsive (33%-BnPAA) polymer was prepared similar to Example # 36. 50 mL of CHO cell culture was placed in a 100 mL glass graduated cylinder in duplicates. In one of the cylinders, the stimulus responsive polymer was added to a dose of 0.5%, the pH was adjusted to 7 with drop wise addition of 2 molar tris base, the sodium
- the sodium phosphate and pH adjustment was performed in order to precipitate the stimulus responsive polymer and a complex of cells, cell debris, impurities, residual polymer; to flocculate the solids; and to increase particle size.
- the sodium phosphate addition and pH adjustment large aggregated particles were observed in the stimulus responsive polymer treated feed.
- the other graduated cylinder was stirred for 2 minutes and nothing was added. Both of the cylinders were allowed to sit undisturbed for 1 hour. At the end of an hour, the supernatant was aspirated and the turbidity was recorded.
- Example 41 Comparison of clarification performance using polvamines of
- a series of polymers with different molecular weights were obtained, modified, and used to flocculate, precipitate, and purify a cell culture.
- Polymers with primary amine repeat units of molecular weights of 15kD, 85kD, 150kD, 350kD, 600- 950kD and 2000-4000kD were obtained and/or modified by the following methods.
- the 15kD polyallylamine polymer was obtained from NITTOBO and was benzylated (covalently attached benzyl groups and purified) using a method similar to example 36.
- the 85kD benzylated polyvinylamine polymer was prepared by Example 23.
- the 150kD benzylated polyallylamine was prepared according to Example 36.
- the 350kD benzylated polyvinylamine polymer was prepared by Example 35.
- the 950kD polyvinylamine polymer backbone is prepared by hydrolyzing Polymin VZ (BASF) with 2 equivalents of base at 80 °C for 8 hours.
- the unmodified 2000-4000kD PVA was prepared according to example 31.
- the benzylated 2000-4000kD polymer was prepared according to Example 32.
- the polymers were used to flocculate, precipitate, and purify a CHO DG44 cell culture of approximately 12xl0 6 cells/mL and a harvest cell viability of ⁇ 50 %.
- the flocculation was performed at polymer doses of 0.2 % and 0.4 % w/w.
- a solution stimulus of 50 mM sodium phosphate and pH adjustment to 7 with 2 molar tris base was applied.
- Observations about the flock size were recorded and are denoted as + being small flocks and M i l l being very large aggregates.
- the vials denoted I I I I were more of a complete zone separation than a suspension of aggregates. It is also noted that the larger aggregates/particles settle faster with a sharper solid liquid interface. Results are shown in Table 9, which demonstrates the results for flocculation using polyamines of different molecular weights.
- CHO cell culture was prepared using a method, as described in
- Example 1 Samples of polymers described in Examples 31, 32, 33 and 35 were added to the cell culture as described in Example 38 with the following exceptions: The polymer dose was between 0.1 wt% and 0.6 wt% as described in Table 10. The initial cell culture turbidity was ⁇ 900 NTU and the centrate without polymer treatment had a turbidity of 212 NTU. The pH was adjusted to 7.2 and a stimulus of 50 mM sodium phosphate was applied. All the conical tubes were centrifuged at 3000 RPM for 2 minutes and the supernatant was decanted and the centrate turbidity was determined.
- Table 10 demonstrates that by changing the nature of the hydrophobic group and/or polymer molecular weight, the response to stimulus and resulting centrate turbidity can be varied.
- DG44 Chinese Hamster Ovary (CHO) cell line expressing PTG1 antibody were grown in a 10L bioreactor (NEW BRUNSWICK SCIENTIFIC) to a density of about 15x106 cells/mL and harvested at ⁇ 50% viability.
- a 10% w/w solution of a hydrophobically modified polyallylamine based stimuli responsive (33%-BnPAA) polymer is prepared similar to Example 36.
- One fraction of the DG44 CHO cell culture is treated with 0.2% of the stimulus responsive polymer 33%-BnPAA, while another fraction of the DG44 CHO cell culture is not treated.
- the sodium phosphate concentration is brought to 50 mM by drop wise addition of 2 molar sodium phosphate and the pH is adjusted to 7.2 by drop wise addition of 2 molar tris base.
- the sodium phosphate and pH adjustment is performed in order to precipitate the stimulus responsive polymer and a complex of cells, cell debris, impurities, residual polymer, and to flocculate the solids, and increase particle size.
- large flocked particles are observed in the stimuus responsive polymer treated feed.
- centrate turbidity for the 33%-BnPAA polymer treated feed was determined to be 10 NTU while the centrate turbidity for the untreated feed was determined to be 60 NTU.
- Depth filter throughput for each feed was determined by the following method.
- the depth filters were equipped with a peristaltic pump and an in-line pressure sensor. The filters were flushed with deionized water according to instructions and the feed was pumped through at 100 LMH and the filtrates were pooled.
- the pooled turbidity for the 33%-BnPAA polymer treated feed filtrate was 6 NTU and the pooled turbidity for the untreated feed filtrate was 9 NTU.
- the filter throughput for the 33%-BnPAA polymer treated feed was 1304 L m2 at 10 psi, at which time the experiment was stopped because of feed limitations.
- the filter throughput for the untreated feed was 206 IJm2 at 20 psi, at which time the experiment was stopped because of pressure limitations.
- Example 44 Purification of a model protein stream with a stimulus responsive polymer followed by capture with a affinity resin
- CHO cell culture is prepared using a method similar to Example 1.
- Initial Feed HCP level ⁇ 210,000ppm.
- a 10% w/w solution of a hydrophobically modified polyallylamine based stimuli responsive (33%-BnPAA) polymer is prepared similar to Example 36.
- One fraction of the CHO cell culture is treated with 0.1% of the stimuli responsive polymer 33%-BnPAA, while another fraction of the CHO cell culture is treated with 0.4% of the stimuli responsive polymer 33%-BnPAA, and a third fraction of the CHO cell culture is not treated.
- the sodium phosphate concentration is brought SO mM by drop wise addition of 2 molar sodium phosphate and the pH is adjusted to 7.2 by drop wise addition of 2 molar tris base.
- the sodium phosphate and pH adjustment is performed in order to precipitate the stimuli responsive polymer and a complex of cells, cell debris, impurities, residual polymer, and to flocculate the solids, and increase particle size.
- large flocked particles are observed in the stimuli responsive polymer treated feeds.
- Each fraction of the cell culture is centrifuged in a lab scale bucket centrifuge for 5 minutes at 3000 RPM. The turbidty of each centrate is recorded and reported in Table 11. The centrates are filtered through a 0.2 ⁇ Durapore® filter.
- the filtrate pools were purified through a three step chromatography based purification consisting of protein A affinity chromatography (ProSep Ultra Plus®), bind-and-elute cation exchange chromatography (ProRes S®), and membrane adsorber anion exchange chromatography in flow-though mode (ChromaSorb ®).
- the purification was performed on a chromatography workstation according to the method in Table 12.
- the pools for each step were analyzed for host cell protein (CHOP) by ELISA, leached Protein A (L ProA) by ELISA, residual DNA by PicoGreen ® assay, turbidity, aggregated protein (AGG) percent by size exclusion HPLC, and protein concentration by UV absorption.
- Example 45 Purification of a model protein stream with a stimulus responsive polymer followed by capture with a cation exchange resin
- DG44 Chinese Hamster Ovary (CHO) cell line expressing PTG1 antibody were grown in a 10L bioreactor (New Brunswick Scientific) to a density of about 15x106 cells/mL and harvested at ⁇ 50% viability with an HCP level of- 142000 ppm.
- a 10% w/w solution of a hydrophobically modified polyallylamine based stimuli responsive (33%-BnPAA) polymer was prepared similar to Example # 36.
- One fraction of the CHO cell culture was treated with 0.1% of the stimuli responsive polymer 33%-BnPAA, while another fraction of the CHO cell culture was treated with 0.4% of the stimuli responsive polymer 33%-BnPAA, and a third fraction of the CHO cell culture was not treated.
- sodium phosphate concentration was brought to 50 mM by drop wise addition of 2 molar sodium phosphate and the pH was adjusted to 7.2 by drop wise addition of 2 molar tris base.
- the sodium phosphate and pH adjustment was performed in order to precipitate the stimuli responsive polymer and a complex of cells, cell debris, impurities, residual polymer, and to flocculate the solids, and increase particle size. After the sodium phosphate addition and pH adjustment large aggregated particles are observed in the stimuli responsive polymer treated feeds. Each fraction of the cell culture is centrifuged in a lab scale bucket centrifuge for 5 minutes at 3000 RPM. The turbidty of each centrate is recorded and reported in Table 13. The centrates were filtered through a 0.2/un Durapore® filter.
- the filtrate pools were purified through a two step chromatography based purification consisting of bind-and-elute cation exchange chromatography (ProRes S®), and membrane adsorber anion exchange chromatography in flow-though mode (ChromaSorb ®).
- the purification was performed on a chromatography workstation according to the method in Table 12.
- the pools for each step were analyzed for host cell protein (CHOP) by ELISA, leached Protein A (L ProA) by ELISA, residual DNA by PicoGreen ® assay, turbidity, aggregated protein (AGG) percent by size exclusion HPLC, and protein concentration by UV absorption.
- Example 46 Polyethylene membrane surface modified with phosphoric acid 2- hvdroxyethylmethacrylate (PAHEMA)
- a membrane was modified to incorporate a multivalent ion stimulus, where the membrane can be used for removal of residual stimulus responsive polymer.
- a 16% aqueous mixture of PAHEMA was prepared according to the following recipe: 16g of PAHEMA (Aldrich #695890, 75% PAHEMA and 25% BisHEMPA), 0.2 g of Irgacure 2959, 93.8 g of water.
- a polyethylene membrane (0.65um, UPDP MILLIPORE) was prewetted with methanol and exchanged into water and treated with the PAHEMA formulation. The sample was exposed to UV light, washed with methanol and water, and dried. The weight added to the membrane by this surface modification was 4.4%. The infrared spectrum of the membrane displayed a strong methacrylate carbonyl absorption. Staining the membrane with methylene blue
- Example 47 HvdrophiHc polyethylene membrane surface modified with phosphoric
- PAHEMA 2-hvdroxyethylmethacrylate
- a 16% aqueous mixture of PAHEMA was prepared according to the following recipe: 16g of PAHEMA (Aldrich #695890, 75% PAHEMA and 25% BisHEMPA), 0.2 g of Irgacure 2959, 93.8 g of water.
- a hydrophiloic membrane (0.65um, MPLC MILLIPORE) was directly contacted with the PAHEMA solution. UV exposure and washing as above gave a membrane with a 7.6% add on. The infrared spectrum of the membrane displayed a strong methacrylate carbonyl absorption.
- Example 48 Hvdrophilic polvmethacrylate resin modified with phosphoric acid hvdroxyethylmethacrylate f PAHEMA
- a resin was modified to include a stimulus, where the modified resin could then be used for removing the residual polymer.
- a solution is prepared with the following composition: 60ml allyl glycidyl ether(AGE), 1 lOg 4M NaOH, 12g sodium sulfate.
- 60ml of ToyoPearl65C media is added and the mixture is placed in a rotating hybridizer at 50C for 16 hours. The media are separated and washed by standard procedures.
- the PAHEMA grafting solution is prepared as follows: l.Og PAHEMA, 0.06g ammonium persulfate, 9.0g water.
- To this solution is added 5ml of the AGE modified ToyoPearl media.
- the mixture is placed in a hybridizer at 80C for 16 hours. Separating and washing by standard procedures gives the PAHEMA modified resin. This product stains dark blue when treated with a 0.01% aqueous solution of methylene blue which is a positively charged dye.
- Example 49 Stimulus responsive polymer binding with an Hvdrophilic polyethylene
- PAHEMA modified MPLC membrane Example 47 was used to capture polyallylamine (PAA) polymer from 3 solutions containing 100, 10, and lppm
- PAA respectively using the following experimental procedure.
- a membrane disk 20mm processed diameter in 15cc cell holder.
- PAA solutions processed at 1.5cc/sec.
- the membrane was washed in and out of cell holder with lOOmL PBS buffer. Processed membrane stained with Ponceau S.
- Ponceau S is a negatively charged dye that absorbs strongly to positively charged surfaces.
- PAA is absorbed by the PAHEMA modified membrane
- the surface is converted from a negative to positive charge. This conversion is easily observed by staining the processed solution with Ponceau S.
- a good measure of degree of staining with Ponceau S is the magenta optical density as measured with a Macbeth Densitometer. The magenta optical density values for various processed membranes (i.e. loaded with PAA) on both the upstream and downstream sides of the membrane are shown in Table 14.
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ES11721929T ES2754210T3 (en) | 2010-05-17 | 2011-05-16 | Stimulus-sensitive polymers for biomolecule purification |
CN201180024500.3A CN102892791B (en) | 2010-05-17 | 2011-05-16 | Stimulus responsive polymers for the purification of biomolecules |
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US9731288B2 (en) | 2017-08-15 |
ES2754210T3 (en) | 2020-04-16 |
KR20160009099A (en) | 2016-01-25 |
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EP3597671B1 (en) | 2022-09-21 |
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CN107312062A (en) | 2017-11-03 |
JP2013529241A (en) | 2013-07-18 |
EP3597671A1 (en) | 2020-01-22 |
CN102892791B (en) | 2017-05-17 |
BR112012028977A2 (en) | 2019-02-12 |
DK2571903T3 (en) | 2019-11-04 |
CN107312062B (en) | 2021-03-16 |
JP5933643B2 (en) | 2016-06-15 |
EP2571903A1 (en) | 2013-03-27 |
SG184541A1 (en) | 2012-11-29 |
US9217048B2 (en) | 2015-12-22 |
JP5612761B2 (en) | 2014-10-22 |
JP2014240391A (en) | 2014-12-25 |
CN102892791A (en) | 2013-01-23 |
KR101827855B1 (en) | 2018-02-12 |
BR112012028977B1 (en) | 2020-05-05 |
US20140171594A1 (en) | 2014-06-19 |
EP2571903B1 (en) | 2019-09-04 |
KR101551295B1 (en) | 2015-09-08 |
KR20150003399A (en) | 2015-01-08 |
JP2016074908A (en) | 2016-05-12 |
US20110313066A1 (en) | 2011-12-22 |
KR101726707B1 (en) | 2017-04-13 |
SG10201804385YA (en) | 2018-06-28 |
JP6055893B2 (en) | 2016-12-27 |
KR20130031832A (en) | 2013-03-29 |
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