WO2011141613A3 - Nueva fitasa, procedimiento de obtencion y utilizacion de la misma - Google Patents

Nueva fitasa, procedimiento de obtencion y utilizacion de la misma Download PDF

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Publication number
WO2011141613A3
WO2011141613A3 PCT/ES2011/070587 ES2011070587W WO2011141613A3 WO 2011141613 A3 WO2011141613 A3 WO 2011141613A3 ES 2011070587 W ES2011070587 W ES 2011070587W WO 2011141613 A3 WO2011141613 A3 WO 2011141613A3
Authority
WO
WIPO (PCT)
Prior art keywords
obtaining same
novel phytase
polypeptide
phytase
soil
Prior art date
Application number
PCT/ES2011/070587
Other languages
English (en)
French (fr)
Other versions
WO2011141613A2 (es
Inventor
Rosa Aligue Alemany
Sergio Atares Real
Ramon Marques Mascarell
Julia Martin Perez
Joaquin Romero Lopez
Ignacio Salaet Madorran
Original Assignee
Fertinagro Nutrientes, S.L.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fertinagro Nutrientes, S.L. filed Critical Fertinagro Nutrientes, S.L.
Priority to EP11763748.8A priority Critical patent/EP2743347A2/en
Priority to PCT/ES2011/070587 priority patent/WO2011141613A2/es
Publication of WO2011141613A2 publication Critical patent/WO2011141613A2/es
Publication of WO2011141613A3 publication Critical patent/WO2011141613A3/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)

Abstract

La invención se refiere a un gen aislado de Serratia odoriferaque codifica una proteína o polipéptido con actividad fitasa, a un método para su obtención en una célula huésped de levaduratal como, por ejemplo Pichia pastoris, y a la utilización de la enzima como ingrediente activo en la fabricación de aditivos para piensos destinados a la nutrición animaly/o para la liberación de fosfato de materia orgánica procedente del suelo.
PCT/ES2011/070587 2011-08-08 2011-08-08 Nueva fitasa, procedimiento de obtencion y utilizacion de la misma WO2011141613A2 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP11763748.8A EP2743347A2 (en) 2011-08-08 2011-08-08 Novel phytase, method for obtaining same and use thereof
PCT/ES2011/070587 WO2011141613A2 (es) 2011-08-08 2011-08-08 Nueva fitasa, procedimiento de obtencion y utilizacion de la misma

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/ES2011/070587 WO2011141613A2 (es) 2011-08-08 2011-08-08 Nueva fitasa, procedimiento de obtencion y utilizacion de la misma

Publications (2)

Publication Number Publication Date
WO2011141613A2 WO2011141613A2 (es) 2011-11-17
WO2011141613A3 true WO2011141613A3 (es) 2012-07-19

Family

ID=44720026

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2011/070587 WO2011141613A2 (es) 2011-08-08 2011-08-08 Nueva fitasa, procedimiento de obtencion y utilizacion de la misma

Country Status (2)

Country Link
EP (1) EP2743347A2 (es)
WO (1) WO2011141613A2 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2617823T3 (pl) 2006-09-21 2015-12-31 Basf Enzymes Llc Fitazy, kodujące je kwasy nukleinowe oraz sposoby ich wytwarzania i stosowania
MX357367B (es) * 2012-10-12 2018-07-04 Univ Autonoma De Nuevo Leon Proceso para la obtencion de preparaciones de polipetidos con actividad de fitasa y su uso.
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
US10093909B2 (en) 2013-07-25 2018-10-09 Basf Enzymes Llc Phytase
EP3018203A1 (en) * 2014-11-06 2016-05-11 Fertinagro Nutrientes, S.L. Novel phytase, method for obtaining the same and use thereof
ES2922623T3 (es) 2015-10-28 2022-09-19 Fertinagro Biotech Sl Tratamiento para mejorar la germinación de semillas y semillas tratadas de esta manera
EP3818835A4 (en) 2018-07-03 2022-01-19 Fertinagro Biotech, S.L. FEED SUPPLEMENT FOR FEED AND USE THEREOF
CN111349576B (zh) * 2018-12-20 2022-05-20 中粮生物科技（北京）有限公司 组成型表达猪胃蛋白酶原a的毕赤酵母工程菌及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
JP5340138B2 (ja) 2006-04-30 2013-11-13 フィード リサーチ インスティチュート チャイニーズ アカデミー オブ アグリカルチュアル サイエンシィズ 新規なフィターゼのクローニング及び発現

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 8 August 2008 (2008-08-08), "Dickeya paradisiaca phytase (appa) gene, complete cds.", XP002674958, retrieved from EBI accession no. EM_PRO:EU086595 Database accession no. EU086595 *
DATABASE UniProt [online] 18 May 2010 (2010-05-18), "SubName: Full=3-phytase; EC=3.1.3.8;", XP002674957, retrieved from EBI accession no. UNIPROT:D4DZK6 Database accession no. D4DZK6 *
HUIYING LUO ET AL: "A Novel Phytase appA from Citrobacter amalonaticus CGMCC 1696: Gene Cloning and Overexpression in Pichia pastoris", CURRENT MICROBIOLOGY, SPRINGER-VERLAG, NE, vol. 55, no. 3, 25 July 2007 (2007-07-25), pages 185 - 192, XP019539731, ISSN: 1432-0991, DOI: 10.1007/S00284-006-0586-4 *
KIM H-W ET AL: "Isolation and characterization of a phytase with improved properties from Citrobacter braakii", BIOTECHNOLOGY LETTERS, KEW, SURREY, GB, vol. 25, no. 15, 1 August 2003 (2003-08-01), pages 1231 - 1234, XP002324049, ISSN: 0141-5492, DOI: 10.1023/A:1025020309596 *

Also Published As

Publication number Publication date
EP2743347A2 (en) 2014-06-18
WO2011141613A2 (es) 2011-11-17

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