WO2011126264A3 - Méthode d'augmentation de l'activité dans les cellules souches humaines - Google Patents

Méthode d'augmentation de l'activité dans les cellules souches humaines Download PDF

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Publication number
WO2011126264A3
WO2011126264A3 PCT/KR2011/002358 KR2011002358W WO2011126264A3 WO 2011126264 A3 WO2011126264 A3 WO 2011126264A3 KR 2011002358 W KR2011002358 W KR 2011002358W WO 2011126264 A3 WO2011126264 A3 WO 2011126264A3
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WO
WIPO (PCT)
Prior art keywords
stem cell
mesenchymal stem
human stem
increasing activity
cell aggregate
Prior art date
Application number
PCT/KR2011/002358
Other languages
English (en)
Korean (ko)
Other versions
WO2011126264A2 (fr
Inventor
김효수
강현재
이은주
Original Assignee
서울대학교병원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020100053183A external-priority patent/KR20110112164A/ko
Application filed by 서울대학교병원 filed Critical 서울대학교병원
Priority to EP11766115A priority Critical patent/EP2557154A2/fr
Priority to CN2011800180070A priority patent/CN102822331A/zh
Priority to US13/639,169 priority patent/US20130028873A1/en
Priority to BR112012025285A priority patent/BR112012025285A2/pt
Priority to AU2011239119A priority patent/AU2011239119A1/en
Publication of WO2011126264A2 publication Critical patent/WO2011126264A2/fr
Publication of WO2011126264A3 publication Critical patent/WO2011126264A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2525/00—Culture process characterised by gravity, e.g. microgravity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne une méthode de préparation d'une cellule souche mésenchymateuse humaine hautement active qui comprend la formation d'un agrégat de cellules sphérique en mettant en culture des cellules souches mésenchymateuses humaines en présence de gravité ; un agrégat de cellules souches hautement actives préparé ainsi ; un produit thérapeutique cellulaire comprenant l'agrégat de cellules souches ; et une méthode de formation d'un agrégat de cellules sphérique en mettant en culture des cellules souches mésenchymateuses humaines dans laquelle la quantité d'E-cadhérine dans la cellule souche mésenchymateuse est augmentée au cours de la culture.
PCT/KR2011/002358 2010-04-05 2011-04-05 Méthode d'augmentation de l'activité dans les cellules souches humaines WO2011126264A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP11766115A EP2557154A2 (fr) 2010-04-05 2011-04-05 Méthode d'augmentation de l'activité dans les cellules souches humaines
CN2011800180070A CN102822331A (zh) 2010-04-05 2011-04-05 提高人干细胞活性的方法
US13/639,169 US20130028873A1 (en) 2010-04-05 2011-04-05 Method for increasing activity in human stem cell
BR112012025285A BR112012025285A2 (pt) 2010-04-05 2011-04-05 método para aumentar a atividade em células-tronco humanas
AU2011239119A AU2011239119A1 (en) 2010-04-05 2011-04-05 Method for increasing activity in human stem cell

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20100031061 2010-04-05
KR10-2010-0031061 2010-04-05
KR1020100053183A KR20110112164A (ko) 2010-04-05 2010-06-07 인간 줄기세포의 활성을 증가시키는 방법
KR10-2010-0053183 2010-06-07

Publications (2)

Publication Number Publication Date
WO2011126264A2 WO2011126264A2 (fr) 2011-10-13
WO2011126264A3 true WO2011126264A3 (fr) 2012-03-08

Family

ID=44763387

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/002358 WO2011126264A2 (fr) 2010-04-05 2011-04-05 Méthode d'augmentation de l'activité dans les cellules souches humaines

Country Status (1)

Country Link
WO (1) WO2011126264A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9534058B2 (en) 2012-02-08 2017-01-03 Abbvie Stemcentrx Llc Anti-CD324 monoclonal antibodies and uses thereof
CN106148266A (zh) * 2015-04-20 2016-11-23 烟台赛泽生物技术有限公司 一种免疫细胞用培养基及该培养基的添加剂
TWI724528B (zh) * 2019-09-04 2021-04-11 三顧股份有限公司 高增生活性的３ｄ結構細胞球體、其製造方法與用途
CN113577394B (zh) * 2021-07-08 2022-09-09 中国人民解放军西藏军区总医院 一种脐带间充质干细胞制备生物凝胶-细胞聚合体体系的方法及低氧条件的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100012153A (ko) * 2008-07-28 2010-02-08 서울대학교병원 인간 줄기세포에서 고활성 줄기세포를 분리하는 방법 및상기 방법에 의해 분리된 고활성 줄기 세포

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100012153A (ko) * 2008-07-28 2010-02-08 서울대학교병원 인간 줄기세포에서 고활성 줄기세포를 분리하는 방법 및상기 방법에 의해 분리된 고활성 줄기 세포

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BARTOSH, T.J. ET AL.: "Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties", PROC. NATL. ACAD. SCI. USA, vol. 107, no. 31, 19 July 2010 (2010-07-19), pages 13724 - 13729, XP055232564, DOI: doi:10.1073/pnas.1008117107 *
FRITH, J.E. ET AL.: "Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential", TISSUE ENGINEERING: PART C, vol. 16, no. 4, 9 November 2009 (2009-11-09), pages 735 - 746 *
WELTER, J.F. ET AL.: "Simplification of aggregate culture of human mesenchymal stem cells as a chondrogenic screening assay", BIOTECHNIQUES, vol. 42, no. 6, June 2007 (2007-06-01), pages 732, 734 - 737, XP008097909, DOI: doi:10.2144/000112451 *
ZHU, H. ET AL.: "A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone", NATURE PROTOCOLS, vol. 5, no. 3, 25 February 2010 (2010-02-25), pages 550 - 560, XP009172805, DOI: doi:10.1038/nprot.2009.238 *

Also Published As

Publication number Publication date
WO2011126264A2 (fr) 2011-10-13

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