WO2011126264A3 - Method for increasing activity in human stem cell - Google Patents

Method for increasing activity in human stem cell Download PDF

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Publication number
WO2011126264A3
WO2011126264A3 PCT/KR2011/002358 KR2011002358W WO2011126264A3 WO 2011126264 A3 WO2011126264 A3 WO 2011126264A3 KR 2011002358 W KR2011002358 W KR 2011002358W WO 2011126264 A3 WO2011126264 A3 WO 2011126264A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cell
mesenchymal stem
human stem
increasing activity
cell aggregate
Prior art date
Application number
PCT/KR2011/002358
Other languages
French (fr)
Korean (ko)
Other versions
WO2011126264A2 (en
Inventor
김효수
강현재
이은주
Original Assignee
서울대학교병원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020100053183A external-priority patent/KR20110112164A/en
Application filed by 서울대학교병원 filed Critical 서울대학교병원
Priority to CN2011800180070A priority Critical patent/CN102822331A/en
Priority to BR112012025285A priority patent/BR112012025285A2/en
Priority to EP11766115A priority patent/EP2557154A2/en
Priority to US13/639,169 priority patent/US20130028873A1/en
Priority to AU2011239119A priority patent/AU2011239119A1/en
Publication of WO2011126264A2 publication Critical patent/WO2011126264A2/en
Publication of WO2011126264A3 publication Critical patent/WO2011126264A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2525/00—Culture process characterised by gravity, e.g. microgravity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Provided are a method for preparing a highly active human mesenchymal stem cell, which includes forming a spherical cell aggregate by cultivating human mesenchymal stem cells against gravity; a highly active stem cell aggregate prepared thereby; a cell theraputic including the stem cell aggregate; and a method for forming a spherical cell aggregate by cultivating human mesenchymal stem cells, wherein the amount of E-cadherin in the mesenchymal stem cell is increased during the cultivation.
PCT/KR2011/002358 2010-04-05 2011-04-05 Method for increasing activity in human stem cell WO2011126264A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN2011800180070A CN102822331A (en) 2010-04-05 2011-04-05 Method for increasing activity in human stem cell
BR112012025285A BR112012025285A2 (en) 2010-04-05 2011-04-05 method to increase activity in human stem cells
EP11766115A EP2557154A2 (en) 2010-04-05 2011-04-05 Method for increasing activity in human stem cell
US13/639,169 US20130028873A1 (en) 2010-04-05 2011-04-05 Method for increasing activity in human stem cell
AU2011239119A AU2011239119A1 (en) 2010-04-05 2011-04-05 Method for increasing activity in human stem cell

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20100031061 2010-04-05
KR10-2010-0031061 2010-04-05
KR10-2010-0053183 2010-06-07
KR1020100053183A KR20110112164A (en) 2010-04-05 2010-06-07 Method for increasing human stem cell activity

Publications (2)

Publication Number Publication Date
WO2011126264A2 WO2011126264A2 (en) 2011-10-13
WO2011126264A3 true WO2011126264A3 (en) 2012-03-08

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/002358 WO2011126264A2 (en) 2010-04-05 2011-04-05 Method for increasing activity in human stem cell

Country Status (1)

Country Link
WO (1) WO2011126264A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9534058B2 (en) 2012-02-08 2017-01-03 Abbvie Stemcentrx Llc Anti-CD324 monoclonal antibodies and uses thereof
CN106148266A (en) * 2015-04-20 2016-11-23 烟台赛泽生物技术有限公司 A kind of immunocyte culture medium and the additive of this culture medium
TWI724528B (en) * 2019-09-04 2021-04-11 三顧股份有限公司 Three-dimensional cell spheroid with high proliferation activity, and the producing method and use therefor
CN113577394B (en) * 2021-07-08 2022-09-09 中国人民解放军西藏军区总医院 Method for preparing biological gel-cell polymer system by umbilical cord mesenchymal stem cells and application of umbilical cord mesenchymal stem cells in low oxygen condition

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100012153A (en) * 2008-07-28 2010-02-08 서울대학교병원 Method for separating high activity stem cells form human stem cells and high activity stem cells separated by the method

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100012153A (en) * 2008-07-28 2010-02-08 서울대학교병원 Method for separating high activity stem cells form human stem cells and high activity stem cells separated by the method

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BARTOSH, T.J. ET AL.: "Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties", PROC. NATL. ACAD. SCI. USA, vol. 107, no. 31, 19 July 2010 (2010-07-19), pages 13724 - 13729, XP055232564, DOI: doi:10.1073/pnas.1008117107 *
FRITH, J.E. ET AL.: "Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential", TISSUE ENGINEERING: PART C, vol. 16, no. 4, 9 November 2009 (2009-11-09), pages 735 - 746 *
WELTER, J.F. ET AL.: "Simplification of aggregate culture of human mesenchymal stem cells as a chondrogenic screening assay", BIOTECHNIQUES, vol. 42, no. 6, June 2007 (2007-06-01), pages 732, 734 - 737, XP008097909, DOI: doi:10.2144/000112451 *
ZHU, H. ET AL.: "A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone", NATURE PROTOCOLS, vol. 5, no. 3, 25 February 2010 (2010-02-25), pages 550 - 560, XP009172805, DOI: doi:10.1038/nprot.2009.238 *

Also Published As

Publication number Publication date
WO2011126264A2 (en) 2011-10-13

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