WO2011122841A3 - 생물학적 시료로부터의 생화학적 물질의 추출용 칼럼 - Google Patents

생물학적 시료로부터의 생화학적 물질의 추출용 칼럼 Download PDF

Info

Publication number
WO2011122841A3
WO2011122841A3 PCT/KR2011/002171 KR2011002171W WO2011122841A3 WO 2011122841 A3 WO2011122841 A3 WO 2011122841A3 KR 2011002171 W KR2011002171 W KR 2011002171W WO 2011122841 A3 WO2011122841 A3 WO 2011122841A3
Authority
WO
WIPO (PCT)
Prior art keywords
column
extracting
solution
biological sample
support
Prior art date
Application number
PCT/KR2011/002171
Other languages
English (en)
French (fr)
Other versions
WO2011122841A2 (ko
Inventor
윤성준
강상현
박지성
박보나
Original Assignee
주식회사 인트론바이오테크놀로지
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 주식회사 인트론바이오테크놀로지 filed Critical 주식회사 인트론바이오테크놀로지
Publication of WO2011122841A2 publication Critical patent/WO2011122841A2/ko
Publication of WO2011122841A3 publication Critical patent/WO2011122841A3/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/043—Hinged closures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Centrifugal Separators (AREA)

Abstract

본 발명은 생물학적 시료로부터의 생화학적 물질을 추출 회수하는 추출용 칼럼에 관한 것으로서, 양단부에 뚜껑과 배출구를 갖는 몸통과, 이 몸통의 내부에 지지구와, 필터, 링플레이트가 순차적으로 삽입되어 설치된 칼럼에 있어서, 칼럼 내면에 접촉되어 고정되는 상기 링플레이트와 지지구의 외주면에는 하나 이상의 홈이 형성된 것을 특징으로 하여, 목적하는 생화학적 물질의 추출 시 칼럼의 몸통 내부로 공급되는 용액(세척액 및 용출액)이 링플레이트와 지지구의 내측 구멍과 외주면에 형성된 홈을 통해서 잔류용액 없이 모두 배출되어 용액의 회수율이 높아짐으로써, 목적하는 생화학적 물질의 추출 시 고순도의 목적 생화학적 물질을 고수율로 회수할 수 있게 된다.
PCT/KR2011/002171 2010-04-02 2011-03-30 생물학적 시료로부터의 생화학적 물질의 추출용 칼럼 WO2011122841A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0030158 2010-04-02
KR1020100030158A KR101069115B1 (ko) 2010-04-02 2010-04-02 생물학적 시료로부터의 생화학적 물질의 추출용 칼럼

Publications (2)

Publication Number Publication Date
WO2011122841A2 WO2011122841A2 (ko) 2011-10-06
WO2011122841A3 true WO2011122841A3 (ko) 2012-03-08

Family

ID=44712748

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/002171 WO2011122841A2 (ko) 2010-04-02 2011-03-30 생물학적 시료로부터의 생화학적 물질의 추출용 칼럼

Country Status (2)

Country Link
KR (1) KR101069115B1 (ko)
WO (1) WO2011122841A2 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2501238B (en) 2012-03-16 2018-10-24 Cambridge Entpr Ltd Methods for obtaining liquid from a solid phase
DE102015102350B3 (de) 2015-02-19 2016-05-04 Sartorius Stedim Biotech Gmbh Filtrationsvorrichtung für flüssige Proben
CN108456678A (zh) * 2018-07-06 2018-08-28 大连元和健路医学检验实验室有限公司 一种快速提取基因组dna的方法
KR102102479B1 (ko) * 2018-10-23 2020-04-20 대한민국 다이엑스 튜브 어셈블리 및 이를 이용한 디엔에이 정제 방법

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050026175A1 (en) * 2003-07-31 2005-02-03 John Link Devices and methods for isolating RNA
KR100845971B1 (ko) * 2007-01-19 2008-07-11 대한민국(관리부서:농촌진흥청) 핵산분리용 컬럼을 이용한 핵산분리 방법
US20080300397A1 (en) * 2007-05-31 2008-12-04 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
KR20090130273A (ko) * 2009-11-16 2009-12-22 주식회사 인트론바이오테크놀로지 생물학적 시료로부터의 생화학적 물질의 추출장치
KR20100001826A (ko) * 2008-06-27 2010-01-06 포항공과대학교 산학협력단 핵산 추출 장치

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050026175A1 (en) * 2003-07-31 2005-02-03 John Link Devices and methods for isolating RNA
KR100845971B1 (ko) * 2007-01-19 2008-07-11 대한민국(관리부서:농촌진흥청) 핵산분리용 컬럼을 이용한 핵산분리 방법
US20080300397A1 (en) * 2007-05-31 2008-12-04 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
KR20100001826A (ko) * 2008-06-27 2010-01-06 포항공과대학교 산학협력단 핵산 추출 장치
KR20090130273A (ko) * 2009-11-16 2009-12-22 주식회사 인트론바이오테크놀로지 생물학적 시료로부터의 생화학적 물질의 추출장치

Also Published As

Publication number Publication date
WO2011122841A2 (ko) 2011-10-06
KR101069115B1 (ko) 2011-09-30

Similar Documents

Publication Publication Date Title
WO2011052910A3 (ko) 원심분리 키트 및 이를 이용한 원심분리 방법
WO2011122841A3 (ko) 생물학적 시료로부터의 생화학적 물질의 추출용 칼럼
WO2012112925A3 (en) Quantitative, highly multiplexed detection of nucleic acids
WO2008134470A3 (en) Methods for nucleic acid amplification
WO2014008487A3 (en) Methods and compositions for separating or enriching cells
USD787700S1 (en) Fluorescent nucleic acid probe detection cartridge assembly unit
WO2007070455A3 (en) Mesporous carbons
EP2139600A1 (en) Apparatus and method for recovering fluid from a fluid absorbing element
MX2008007858A (es) Recuperacion de componentes residuales de plantas despues de la destilacion de aceites esenciales.
WO2011087218A3 (en) Apparatus for analyzing sample
WO2009108567A3 (en) Sample pretreatment and extraction
GB201318942D0 (en) Methods and devices for nucleic acid purification
WO2011136606A3 (ko) 버피코트 추출 키트 및 방법
EA201400853A1 (ru) Способы извлечения и/или удаления реагентов из пористой среды
MX338076B (es) Deteccion cuantitativa, altamente multiplexada de acidos nucleicos.
NZ600683A (en) Reactor comprising a rotor
WO2009143241A3 (en) Differentiation of multi-lineage progenitor cells to pancreatic cells
EG24648A (en) A process for extracting biological material selected from sugar beet
WO2012075438A3 (en) Processing assembly for semiconductor workpiece and mehtods of processing same
WO2011140270A3 (en) Methods and systems for ultra-trace analysis of liquids
WO2010100492A3 (en) Methods and apparatus for the production of carbon-containing materials
FR2931229B1 (fr) Procede automatise et securise de preparation de munitions cylindriques en vue de leur destruction et de destruction de ces munitions
BRPI0908601A2 (pt) separador destinado a receber de modo contínuo um fluido sob pressão, dispositivo de extração de um material situado no fundo de uma extensão de água e processo de separação de modo contínuo de um fluido sob pressão com o auxílio de um separador.
WO2014064542A3 (en) Micro-scale liquid-liquid-liquid extraction
GB2539857A (en) Devices and methods for purification, detection and use of biological cells

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11763013

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11763013

Country of ref document: EP

Kind code of ref document: A2