GB2539857A - Devices and methods for purification, detection and use of biological cells - Google Patents
Devices and methods for purification, detection and use of biological cells Download PDFInfo
- Publication number
- GB2539857A GB2539857A GB1618356.8A GB201618356A GB2539857A GB 2539857 A GB2539857 A GB 2539857A GB 201618356 A GB201618356 A GB 201618356A GB 2539857 A GB2539857 A GB 2539857A
- Authority
- GB
- United Kingdom
- Prior art keywords
- column
- cells
- methods
- devices
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/428—Frontal mode
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
- B01D15/166—Fluid composition conditioning, e.g. gradient
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/422—Displacement mode
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer. Purified viable cells can be detected on the column or eluted from the column and detected. Cells on a column can be used as a stationary phase for liquid chromatography. Cells may be removed, recovered and analyzed.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB2102155.5A GB2587768B (en) | 2014-04-03 | 2015-04-03 | Devices and methods for purification, detection and use of biological cells |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201461974950P | 2014-04-03 | 2014-04-03 | |
US201462061636P | 2014-10-08 | 2014-10-08 | |
PCT/US2015/024374 WO2015154048A1 (en) | 2014-04-03 | 2015-04-03 | Devices and methods for purification, detection and use of biological cells |
Publications (3)
Publication Number | Publication Date |
---|---|
GB201618356D0 GB201618356D0 (en) | 2016-12-14 |
GB2539857A true GB2539857A (en) | 2016-12-28 |
GB2539857B GB2539857B (en) | 2021-03-31 |
Family
ID=54241352
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB1618356.8A Active GB2539857B (en) | 2014-04-03 | 2015-04-03 | Devices and methods for purification, detection and use of biological cells |
GB2102155.5A Active GB2587768B (en) | 2014-04-03 | 2015-04-03 | Devices and methods for purification, detection and use of biological cells |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB2102155.5A Active GB2587768B (en) | 2014-04-03 | 2015-04-03 | Devices and methods for purification, detection and use of biological cells |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP3126027A4 (en) |
GB (2) | GB2539857B (en) |
WO (1) | WO2015154048A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB201521784D0 (en) * | 2015-12-10 | 2016-01-27 | Univ Birmingham | Cell purification |
CA3123030C (en) * | 2018-10-09 | 2023-04-04 | C Technologies, Inc. | Mass control system for chromatography |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5215927A (en) * | 1986-01-30 | 1993-06-01 | Fred Hutchinson Cancer Research Center | Method for immunoselection of cells using avidin and biotin |
US6362008B1 (en) * | 1997-12-23 | 2002-03-26 | Barbara A. Kohn | Generic signalling mechanism for detection of analytes |
US20040121382A1 (en) * | 2002-12-18 | 2004-06-24 | Linda Liu | Multiplexed immunohistochemical assays using molecular tags |
WO2013124474A2 (en) * | 2012-02-23 | 2013-08-29 | Stage Cell Therapeutics Gmbh | Chromatographic isolation of cells and other complex biological materials |
WO2014001326A1 (en) * | 2012-06-27 | 2014-01-03 | F. Hoffmann-La Roche Ag | Method for the selection and production of tailor-made, selective and multi-specific therapeutic molecules comprising at least two different targeting entities and uses thereof |
US20140234890A1 (en) * | 2013-02-15 | 2014-08-21 | Douglas T. Gjerde | Methods for Purifying Biological Cells |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5773224A (en) * | 1996-02-12 | 1998-06-30 | Grandics; Peter | Immunoselection system for cell elution |
US20090081084A1 (en) * | 2003-07-14 | 2009-03-26 | Gjerde Douglas T | Low Dead Volume Extraction Column Device |
US8377715B2 (en) * | 2003-07-14 | 2013-02-19 | Phynexus, Inc. | Method and device for sample preparation |
US20100140173A1 (en) * | 2004-12-03 | 2010-06-10 | Chris Suh | Method and Device for Gravity Flow Chromatography |
GB2525562B (en) * | 2013-02-15 | 2017-10-11 | T Gjerde Douglas | Methods for purification of biological cells |
-
2015
- 2015-04-03 WO PCT/US2015/024374 patent/WO2015154048A1/en active Application Filing
- 2015-04-03 EP EP15773853.5A patent/EP3126027A4/en not_active Withdrawn
- 2015-04-03 GB GB1618356.8A patent/GB2539857B/en active Active
- 2015-04-03 GB GB2102155.5A patent/GB2587768B/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5215927A (en) * | 1986-01-30 | 1993-06-01 | Fred Hutchinson Cancer Research Center | Method for immunoselection of cells using avidin and biotin |
US6362008B1 (en) * | 1997-12-23 | 2002-03-26 | Barbara A. Kohn | Generic signalling mechanism for detection of analytes |
US20040121382A1 (en) * | 2002-12-18 | 2004-06-24 | Linda Liu | Multiplexed immunohistochemical assays using molecular tags |
WO2013124474A2 (en) * | 2012-02-23 | 2013-08-29 | Stage Cell Therapeutics Gmbh | Chromatographic isolation of cells and other complex biological materials |
WO2014001326A1 (en) * | 2012-06-27 | 2014-01-03 | F. Hoffmann-La Roche Ag | Method for the selection and production of tailor-made, selective and multi-specific therapeutic molecules comprising at least two different targeting entities and uses thereof |
US20140234890A1 (en) * | 2013-02-15 | 2014-08-21 | Douglas T. Gjerde | Methods for Purifying Biological Cells |
Also Published As
Publication number | Publication date |
---|---|
GB2539857B (en) | 2021-03-31 |
GB2587768A (en) | 2021-04-07 |
GB2587768B (en) | 2021-08-18 |
GB202102155D0 (en) | 2021-03-31 |
GB201618356D0 (en) | 2016-12-14 |
EP3126027A1 (en) | 2017-02-08 |
WO2015154048A1 (en) | 2015-10-08 |
EP3126027A4 (en) | 2017-11-01 |
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