WO2011105884A2 - Appareil et procédé destinés à distribuer des échantillons liquides dans des petits volumes - Google Patents

Appareil et procédé destinés à distribuer des échantillons liquides dans des petits volumes Download PDF

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Publication number
WO2011105884A2
WO2011105884A2 PCT/MX2011/000032 MX2011000032W WO2011105884A2 WO 2011105884 A2 WO2011105884 A2 WO 2011105884A2 MX 2011000032 W MX2011000032 W MX 2011000032W WO 2011105884 A2 WO2011105884 A2 WO 2011105884A2
Authority
WO
WIPO (PCT)
Prior art keywords
deposits
ducts
liquid
duct
communicating
Prior art date
Application number
PCT/MX2011/000032
Other languages
English (en)
Spanish (es)
Other versions
WO2011105884A3 (fr
Inventor
José Carlos BARRENECHEA OAR
Raúl GRANDE GUTIERREZ
Original Assignee
Barrenechea Oar Jose Carlos
Grande Gutierrez Raul
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from MX2010002038A external-priority patent/MX2010002038A/es
Application filed by Barrenechea Oar Jose Carlos, Grande Gutierrez Raul filed Critical Barrenechea Oar Jose Carlos
Priority to KR1020127024985A priority Critical patent/KR20130121675A/ko
Priority to US13/580,588 priority patent/US20130059329A1/en
Priority to CN2011800202686A priority patent/CN103097028A/zh
Priority to EP11747771A priority patent/EP2540395A2/fr
Priority to JP2012554952A priority patent/JP2013520677A/ja
Priority to BR112012021124-2A priority patent/BR112012021124A2/pt
Publication of WO2011105884A2 publication Critical patent/WO2011105884A2/fr
Publication of WO2011105884A3 publication Critical patent/WO2011105884A3/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation

Definitions

  • the invention consists of a rigid and preformed duct of non-permeable material, with an inlet and one or more outlets in which, due to gravity and air displacement, automatic distribution of liquids shown in preformed tanks or compartments is achieved in the same pipeline.
  • the dimensions of the pipeline vary throughout it, forming communicating ducts, deposits and, where appropriate, bifurcations.
  • the pipeline is located in such a way that the entrance and the exit (s) are at a higher level and below it, two or more deposits equal or not to each other which can be located:
  • Each tank has a communicating duct between itself and the liquid inlet shows as well as an outlet duct for the displaced air that reaches the top of the apparatus
  • the dimensions of the deposits are calculated based on the total number of parts in which it is desired to divide the sample, the total volume of the sample and quantity of air and liquid shows that it is desired to leave in each of the deposits and even the geometric shape that you want to use for each deposit, these being the same or different from each other.
  • the level of height at which the communicating ducts enter and leave the tanks is determined by the required proportion or percentage of sample liquid and air that is desired to remain in each of them and may even leave it without air. Said level can be the same in all the deposits or different between them, depending on the needs of the user and is calculated taking into account the device in an upright position.
  • the communicating ducts can always be located on the same side in all the tanks in such a way that when the device is laid on the opposite side where the ducts are located, the liquid shown by gravity effects is evacuated from the communicating ducts and housed in the tanks, leaving the liquid shows, in addition to divided, totally separated and independent.
  • Aerotolerant anaerobes which can grow in the presence or absence of oxygen, but their metabolism is always fermentative.
  • the most commonly used methods for the quantification of bacteria and other microorganisms in water are that of Membrane Filtration and the Most Probable Number (NMP) Method.
  • the first somewhat more limited than the second because of its difficulty in quantification when the number of colonies is very high.
  • the NMP procedure is based on a statistical estimate of the number of bacteria per unit volume and is determined by the number of positive results in a series of tubes or tanks incubated. The greater the number of deposits incubated, the greater the accuracy of the results obtained. Also, when the bacterial counts are very high, this method allows dilutions to be made to find effective readings that allow a clear idea of the bacterial density of the sample liquid.
  • a simple and quick method to separate the liquid shows in many small homogeneous portions as well. as the possibility of counting or not, depending on the case with air in each of the tanks or culture compartments is very useful to achieve optimal results.
  • the estimation of bacterial density by means of the NMP method can be done in two ways, one that is the determination by means of multiple tubes as described in the "Standard Methods for the Examination of Water and Wastewater" using test tubes performing the separation of the sample liquid manually by means of pipettes or similar apparatus and also using the method described in US Pat. 5,518,892, US 5,620,895, US 5,753,456.
  • an apparatus is used for the separation of the sample consisting of a bag in which the sample liquid is divided by the mechanical action of rollers that at the same time provide the heat necessary to adhere a film to the rigid side of the bag achieving the total separation of the sample.
  • This option does not allow the alternative of leaving a specific volume of air in each of the aliquots and also requires special equipment for its division and separation.
  • Another advantage is that, in the case of the tanks containing sufficient air and also the design of the tanks being adequate, the liquid of each tank is separated from the rest with the simple fact of laying down or turning said apparatus.
  • the separation between the compartments could also be obtained with the simple sealing of the communicating ducts that have the deposits with each other by applying pressure and heat at specific points.
  • Figure 1. A figure is shown with a vertical front view in which independent deposits are observed with the following components: a. - Communicating channels
  • Figure 3. It shows that two or more series can be integrated to achieve a greater number of independent tanks, each series with its independent outlet duct.
  • Figure 4. Shows the location, on one side, of the deposits. In order to evacuate the communicating ducts, these can be located on the side of the tanks in such a way that when the device is rotated, the liquid contained in them due to gravity, is evacuated from the communicating ducts and housed in the tanks achieving in addition to the division the complete separation of the liquid sample.
  • Figure 5. Shows a view of a first embodiment of the present invention.
  • Figure 6.- Shows a view of a second embodiment of the present invention.
  • the invention consists of a rigid and preformed duct of non-permeable material, with an inlet and one or more outlets in which, due to the effects of gravity and air displacement, the automatic distribution of liquids shown in preformed tanks or compartments is achieved.
  • pipeline The dimensions of the pipeline vary along it, forming communicating ducts, bifurcations and deposits, which are located in such a way that they allow a controlled flow of air and the accommodation of the liquid shows in the deposits formed within it.
  • Each tank has a communicating duct between itself and the liquid inlet shows as well as an outlet duct for the displaced air that reaches the top of the apparatus
  • the dimensions of the deposits are calculated based on the total number of parts in which you want to divide the sample, the total volume of the sample and the amount of air and liquid that you want to leave in each of the deposits and even the shape geometric that you want to use for each deposit and these can be the same or different from each other.
  • the level of height at which the communicating ducts enter and leave the tanks is determined by the required proportion or percentage of sample liquid and air that is desired to remain in each of them and may even leave it without air. Said level can be the same in all the deposits or different between them, depending on the needs of the user and is calculated taking into account the device in an upright position.
  • the communicating ducts can always be located on the same side in all the tanks in such a way that when the device is laid on the opposite side where the ducts are located, the liquid shows by gravity, it is evacuated from the communicating ducts and housed in the tanks, leaving the liquid shows, in addition to divided, totally separated and independent.
  • Deposits should always be located below the level of the entrance to the pipeline as well as the exit (s) thereof, regardless of position.
  • each tank and ducts can be adjusted to the dimensions most appropriate to the production process, geometric shape or the desired design as long as they have the required volume including the desired amount of air for each tank and considering the device in an upright position.
  • the sum of the volume of the ducts, the deposits (whether they are equal or not with each other), is equal to the total volume of the sample plus the percentage of air desired.
  • the height at which the upper part of the communicating ducts of each tank is located is determined by obtaining the level of height that the liquid shown in each tank will take, taking into account the volume of the sample liquid contained in the ducts, placing the latter of preference on the same side of deposits in all cases.
  • the pipeline is placed vertically, with the liquid inlet shown at the top.
  • the sample liquid is poured into the pipeline and, due to gravity, is distributed in each of the smaller deposits through the lower communicating ducts.
  • the air contained in these, it is displaced by the liquid and leaves through the communicating conduits that rise, allowing the defined filling for each of the deposits.
  • the width of the tanks should be sufficient and proportionally greater than the length thereof, so that By simply laying down the device, the communicating ducts remain at the top of the device, causing the liquid to be located in the ducts, due to gravity, it is dislodged from the ducts and located in the tanks where the final volume of the liquid Sample of each deposit does not reach the level in which the communicating ducts are found thus achieving, in addition to the division, the separation of the liquid sample.
  • the deposits could be definitively insulated by blocking the passage of all the communicating ducts using an apparatus that applies heat and pressure exclusively at specific points of the communicating ducts.
  • Example 1 (FIGURE 5) of apparatus for quantification of facultative aerobic and anaerobic bacteria by means of NMP 72 Deposits.
  • An example of this apparatus is a duct of a material such as polyvinyl chloride (PVC), polyethylene terephthalate (PET), polyester, ethylene vinyl acetate (EVA), polyethylene (PE), polypropylene (PP) or similar, or a combination of them, thermoplastic or thermofix, transparent, composed of two plates, one of which has a part of the duct thermoformed with the desired shapes and dimensions and the other complementary plate, attached to the first, finishing forming the duct.
  • the design of the duct on the thermoformed plate includes: a larger reservoir for the distribution of the liquid that is located at the top and that has a volume of 100 ml and which in turn has an upper opening through which the sample liquid will be introduced .
  • each tank has a volume of 1.95 mi and with the following dimensions lcm x 1.2cm x 1.625 cm.
  • the deposits of each level are connected to its neighbor by means of communicating ducts in the form of half a rod with a diameter of 0.7 cm and that are located at a height of 1.15 cm with respect to the bottom of the tank which allows each tank to contain a volume of 1.38 ml of liquid sample and a percentage of 30% of air.
  • the first of the tanks of each series is connected by means of a conduit of diameter of 0.7cm to one of two vertical conduits of 0.7 of diameter that in turn connects them to the greater deposit, the first of these for the first two series and the second for the remaining.
  • a communicating duct in the form of half a cane and 0.3 cm in diameter goes to the top of the plate which allows the excess air to be allowed allowing hence the flow of the liquid shows through the deposits of the same level.
  • thermoformed plate that joins by means of heat and / or pressure and / or an adhesive to a complementary plate leaving the two parts together forming a rigid and waterproof duct.
  • the contour is cut and the communicating conduits that facilitate the air outlet as well as the liquid inlet shows the upper tank. Once finished, it is sterilized to carry out tests such as those of the NMP using specific reagents for each type of microorganism.
  • the device is placed vertically, with the larger reservoir and liquid inlet shown on the top.
  • the sample liquid is poured into said reservoir and due to the effects of gravity and air displacement, it is distributed in each of the smaller reservoirs through the communicating ducts.
  • the liquid shown in each smaller tank remains in the lower part, allowing the percentage of the desired air to remain inside it, being located above the level at which the communicating ducts are located.
  • the excess air contained in these leaves through the communicating ducts to the neighboring tank and finally through the ducts that rise to the side of the upper tank allowing the distribution of the liquid shown in the smaller deposits.
  • the device placing the communicating ducts at the top which will cause the remaining air in each tank to isolate the liquid from them by avoiding contact with the liquid from the adjacent tanks.
  • the deposits could be definitively insulated by blocking the passage of all the communicating ducts using an apparatus that applies heat and pressure exclusively at specific points of the communicating ducts.
  • Example 2 (FIGURE 6) of an apparatus for quantification of Aerobic and Anaerobic Optional Bacteria by means of MP 75 Communicated, Independent Deposits
  • An example of this apparatus is a pipeline of polyvinylchloride (PVC) material, polyethylene terephthalate (PET), polyester , ethylene vinyl acetate (EVA), polyethylene
  • thermoformed plate consisting of two plates, one of which has a thermoformed part of the duct with the shapes and desired dimensions and the other complementary plate, attached to the first, finishing forming the duct.
  • the design of the duct on the thermoformed plate includes: a larger reservoir for the distribution of the liquid that is located at the top and with a volume of 100 ml and which in turn has a top opening through which the sample liquid will be introduced.
  • Each tank has a predetermined volume with a specific liquid and air ratio.
  • the tanks of each level are connected to a contiguous tank by means of communicating conduits that are located at a predetermined height with respect to the lower part of the tank which allows each tank to contain a specific volume of sample liquid and a percentage of air.
  • the first of the tanks of each series is connected by means of a conduit to the horizontal part of the main distribution conduit that exits the greater deposit.
  • a communicating duct is released that is connected to the main air outlet duct and from there, towards the top of the apparatus which allows the exit of the excess air thus allowing the flow of the liquid shows through the deposits.
  • thermoformed plate that joins by means of heat and / or pressure and / or an adhesive to another complementary plate, leaving the two parts together forming a rigid and impermeable duct.
  • the contour is cut by means of a swell and the inlet is opened to the outside where the liquid is poured into the upper tank and the communicating duct that allows the air to escape. Once finished, it is sterilized to carry out tests such as that of the NMP using specific reagents for each type of microorganisms.
  • the device is placed vertically, with the larger reservoir and liquid inlet shown on the top.
  • the sample liquid is poured into said reservoir and due to the effects of gravity and air displacement it is distributed in each of the smaller reservoirs at different levels through the communicating ducts.
  • the liquid shown in each smaller tank remains in the lower part, allowing the percentage of the desired air to remain inside it when it is located above the level at which it is They find the communicating ducts.
  • the excess air contained in these leaves through the communicating ducts to the tank located above it and finally through the ducts that rise to the main air outlet duct allowing the distribution of the liquid shown in the smaller deposits.
  • the apparatus placing the communicating conduits in the part upper, which will generate that the liquid shows content in the communicating ducts between the tanks flow and is deposited in the corresponding compartment leaving said duct free of liquid.
  • the liquid sample contained in the main distribution conduit is distributed in the three additional tanks.
  • the device lies down with the tanks facing down, placing the ducts on the upper face.
  • the deposits could be definitively insulated by blocking the passage of all the communicating ducts using an apparatus that applies heat and pressure exclusively at specific points of the communicating ducts. If it is desired to vary the proportion of the liquid-air in each tank, it is sufficient to vary the inclination of the apparatus from the vertical at the time of pouring the sample liquid.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un appareil destiné à distribuer des échantillons liquides dans des petits volumes, qui comprend un canal rigide préformé de matière non perméable, avec une entrée et une ou plusieurs sorties, caractérisé en ce que les dimensions du canal varient entre elles, formant des conduits communiquants, des dérivations et des dépôts; et un procédé de fabrication correspondant.
PCT/MX2011/000032 2010-02-22 2011-02-22 Appareil et procédé destinés à distribuer des échantillons liquides dans des petits volumes WO2011105884A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
KR1020127024985A KR20130121675A (ko) 2010-02-22 2011-02-22 시료 액체를 소량으로 분배하는 장치 및 방법
US13/580,588 US20130059329A1 (en) 2010-02-22 2011-02-22 Apparatus and method for distributing liquid samples in small volumes
CN2011800202686A CN103097028A (zh) 2010-02-22 2011-02-22 用于以小体积分配样本液体的装置和方法
EP11747771A EP2540395A2 (fr) 2010-02-22 2011-02-22 Appareil et procédé destinés à distribuer des échantillons liquides dans des petits volumes
JP2012554952A JP2013520677A (ja) 2010-02-22 2011-02-22 試料液体を少ない容量で分配するための装置および方法
BR112012021124-2A BR112012021124A2 (pt) 2010-02-22 2011-02-22 aparelho e método para distribuição de amostras de líquido em pequenos volumes.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MX2010002038A MX2010002038A (es) 2007-08-29 2008-08-19 Molino de rodillo para moler material solido.
MXMX/A/2010/002036 2010-02-22

Publications (2)

Publication Number Publication Date
WO2011105884A2 true WO2011105884A2 (fr) 2011-09-01
WO2011105884A3 WO2011105884A3 (fr) 2012-03-22

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MX2011/000032 WO2011105884A2 (fr) 2010-02-22 2011-02-22 Appareil et procédé destinés à distribuer des échantillons liquides dans des petits volumes

Country Status (1)

Country Link
WO (1) WO2011105884A2 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5518892A (en) 1994-02-23 1996-05-21 Idexx Laboratories, Inc. Apparatus and method for quantification of biological material in a liquid sample

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5609828A (en) * 1995-05-31 1997-03-11 bio M erieux Vitek, Inc. Sample card
FR2782729B1 (fr) * 1998-09-01 2002-10-25 Bio Merieux Carte de denombrement et de caracterisation de micro-organismes
US6637463B1 (en) * 1998-10-13 2003-10-28 Biomicro Systems, Inc. Multi-channel microfluidic system design with balanced fluid flow distribution
DE10302721A1 (de) * 2003-01-23 2004-08-05 Steag Microparts Gmbh Mikrofluidische Anordnung zum Dosieren von Flüssigkeiten

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5518892A (en) 1994-02-23 1996-05-21 Idexx Laboratories, Inc. Apparatus and method for quantification of biological material in a liquid sample
US5620895A (en) 1994-02-23 1997-04-15 Idexx Laboratories, Inc. Apparatus for quantification of biological material in a liquid sample
US5753456A (en) 1994-02-23 1998-05-19 Idexx Laboratiories, Inc. Method for quantification of biological material in a liquid sample

Also Published As

Publication number Publication date
WO2011105884A3 (fr) 2012-03-22

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