WO2011093606A3 - Pna 기반의 실시간 pcr 클램핑을 이용한 braf 돌연변이 검출 방법 및 키트 - Google Patents

Pna 기반의 실시간 pcr 클램핑을 이용한 braf 돌연변이 검출 방법 및 키트 Download PDF

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WO2011093606A3
WO2011093606A3 PCT/KR2011/000251 KR2011000251W WO2011093606A3 WO 2011093606 A3 WO2011093606 A3 WO 2011093606A3 KR 2011000251 W KR2011000251 W KR 2011000251W WO 2011093606 A3 WO2011093606 A3 WO 2011093606A3
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pna
kit
real
basis
time pcr
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PCT/KR2011/000251
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French (fr)
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WO2011093606A2 (ko
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박희경
최재진
조민혜
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주식회사 파나진
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Publication of WO2011093606A2 publication Critical patent/WO2011093606A2/ko
Publication of WO2011093606A3 publication Critical patent/WO2011093606A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 BRAF유전자의 코돈 600의 야생형과 특이적으로 결합하는 PNA(Peptide Nucleic Acid) 프로브를 이용하여 돌연변이만을 선택적으로 검출하는 방법 및 상기 방법을 사용하기 위한 키트에 관한 것으로, 갑상선암을 비롯하여 악성 흑색종, 난소암, 대장암 등의 종양을 조기에 신속하고 정확하게 검사할 수 있어, 조기 암 진단으로 인한 효율적인 치료를 가능하게 할 것이다.
PCT/KR2011/000251 2010-02-01 2011-01-13 Pna 기반의 실시간 pcr 클램핑을 이용한 braf 돌연변이 검출 방법 및 키트 WO2011093606A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0009041 2010-02-01
KR1020100009041A KR101825117B1 (ko) 2010-02-01 2010-02-01 Ｐｎａ 기반의 실시간 ｐｃｒ 클램핑을 이용한 ｂｒａｆ 돌연변이 검출 방법 및 키트

Publications (2)

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WO2011093606A2 WO2011093606A2 (ko) 2011-08-04
WO2011093606A3 true WO2011093606A3 (ko) 2011-12-01

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PCT/KR2011/000251 WO2011093606A2 (ko) 2010-02-01 2011-01-13 Pna 기반의 실시간 pcr 클램핑을 이용한 braf 돌연변이 검출 방법 및 키트

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WO (1) WO2011093606A2 (ko)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10077474B2 (en) 2012-05-29 2018-09-18 Abbott Molecular, Inc. Method of designing primers, method of detecting single nucleotide polymorphisms (SNPs), method of distinguishing SNPs, and related primers, detectable oligonucleotides, and kits
CN103571948B (zh) * 2013-10-09 2015-03-25 武汉百泰基因工程有限公司 一种用于检测braf基因热点突变的试剂盒及其检测方法
CN104928355A (zh) * 2014-03-19 2015-09-23 李跃 检测braf基因突变的方法及其试剂盒
CN104031992B (zh) * 2014-05-27 2016-03-09 武汉海吉力生物科技有限公司 人类B-raf基因V600突变检测试剂盒
US20180187267A1 (en) 2017-01-05 2018-07-05 Michael J. Powell Method for conducting early detection of colon cancer and/or of colon cancer precursor cells and for monitoring colon cancer recurrence
US10400277B2 (en) * 2014-06-10 2019-09-03 Diacarta Ltd DNA mutation detection employing enrichment of mutant polynucleotide sequences and minimally invasive sampling

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080176226A1 (en) * 2007-01-19 2008-07-24 Chang Gung University Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both pcr clamp and sensor probe
US20080268449A1 (en) * 2007-02-26 2008-10-30 John Wayne Cancer Institute Utility of b-raf dna mutation in diagnosis and treatment of cancer

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080176226A1 (en) * 2007-01-19 2008-07-24 Chang Gung University Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both pcr clamp and sensor probe
US20080268449A1 (en) * 2007-02-26 2008-10-30 John Wayne Cancer Institute Utility of b-raf dna mutation in diagnosis and treatment of cancer

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KYGER ET AL.: "Detection of the hereditary hemochromatosis gene mutation by real-time fluorescence polymerase chain reaction and peptide nucleic acid clamping", ANALYTICAL BIOCHEMISTRY, vol. 260, no. 2, 1 July 1998 (1998-07-01), pages 142 - 148 *
MIYAKE ET AL.: "Sensitive detection of FGFR3 mutations in bladder cancer and urine sediments by peptide nucleic acid-mediated real-time PCR clamping", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 362, no. 4, 27 August 2007 (2007-08-27), pages 865 - 871 *

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KR101825117B1 (ko) 2018-02-06
WO2011093606A2 (ko) 2011-08-04
KR20110089577A (ko) 2011-08-09

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