WO2011081434A3 - 세포별 흡광 이미지 분석을 이용한 세포사멸 정량분석법 - Google Patents

세포별 흡광 이미지 분석을 이용한 세포사멸 정량분석법 Download PDF

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Publication number
WO2011081434A3
WO2011081434A3 PCT/KR2010/009483 KR2010009483W WO2011081434A3 WO 2011081434 A3 WO2011081434 A3 WO 2011081434A3 KR 2010009483 W KR2010009483 W KR 2010009483W WO 2011081434 A3 WO2011081434 A3 WO 2011081434A3
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Prior art keywords
cell
light absorption
apoptosis
analysis
analysis method
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PCT/KR2010/009483
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English (en)
French (fr)
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WO2011081434A2 (ko
Inventor
윤태현
임국희
김민정
박종훈
유현주
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한양대학교 산학협력단
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Priority claimed from KR1020100136734A external-priority patent/KR101272884B1/ko
Application filed by 한양대학교 산학협력단 filed Critical 한양대학교 산학협력단
Publication of WO2011081434A2 publication Critical patent/WO2011081434A2/ko
Publication of WO2011081434A3 publication Critical patent/WO2011081434A3/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/272—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's

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  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Molecular Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Mathematical Physics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

본 발명은 흡광 이미지 분석을 이용한 세포사멸 정량분석법에 관한 것으로, 보다 구체적으로는 화학시료에 노출되어 세포사멸이 유도된 단일 세포층에 흡광염료 처리를 한 후 형성된 이미지를 촬영하고 각세포별 흡광도를 정량적으로 분석하는 세포사멸 정량분석법에 관한 것이다. 본 발명에 따르면 세포별 흡광 및 형태 인자의 다중변수분석을 이용하여 정상세포와 대비하여 개별 세포가 퇴화되는 사멸의 과정을 MTT 검색법등 기존의 흡광기반 세포 사멸 분석법들에 비하여 보다 정확하게 정량화 할 수 있으므로 고효율, 고집적도의 세포기반 미세유체칩, 신약개발시 사전 인 비트로 스크리닝(in-vitro screening) 장치, 유해 화학물질 세포독성 평가장치 등으로 활용될 수 있다.
PCT/KR2010/009483 2009-12-29 2010-12-29 세포별 흡광 이미지 분석을 이용한 세포사멸 정량분석법 WO2011081434A2 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20090133151 2009-12-29
KR10-2009-0133151 2009-12-29
KR1020100136734A KR101272884B1 (ko) 2009-12-29 2010-12-28 세포별 흡광 이미지 분석을 이용한 세포사멸 정량분석법
KR10-2010-0136734 2010-12-28

Publications (2)

Publication Number Publication Date
WO2011081434A2 WO2011081434A2 (ko) 2011-07-07
WO2011081434A3 true WO2011081434A3 (ko) 2011-12-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/009483 WO2011081434A2 (ko) 2009-12-29 2010-12-29 세포별 흡광 이미지 분석을 이용한 세포사멸 정량분석법

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WO (1) WO2011081434A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105209595B (zh) * 2013-05-02 2017-03-08 昆塔麦特利斯株式会社 微流体多孔型细胞培养测试装置
KR101396110B1 (ko) 2013-10-30 2014-05-16 아주대학교산학협력단 장쇄 분지를 갖는 지방족 폴리카보네이트 및 이의 방향족 폴리에스터 공중합체
CN111321078B (zh) * 2020-03-05 2023-12-22 吉林大学 细胞培养箱及其控制方法和培养皿

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040229377A1 (en) * 2003-04-14 2004-11-18 National Cheng Kung University Microfluidic chip system integrated with nano-electrospray interface and method using thereof
KR20060080353A (ko) * 2005-01-05 2006-07-10 한국과학기술원 렌즈 없는 칩위의 광학 현미경 및 이를 이용한 영상 획득시스템

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040229377A1 (en) * 2003-04-14 2004-11-18 National Cheng Kung University Microfluidic chip system integrated with nano-electrospray interface and method using thereof
KR20060080353A (ko) * 2005-01-05 2006-07-10 한국과학기술원 렌즈 없는 칩위의 광학 현미경 및 이를 이용한 영상 획득시스템

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CRISTINA M.P. ET AL.: "Confocal restricted height imaging of suspension cells (CRISC) in a PDMS microdevice during apoptosis.", LAB CHIP., vol. 5, 2005, pages 628 - 33 *
DE WITT HAMER P.C. ET AL.: "Quantification of viability in organotypic multicellular spheroids of human malignant glioma using lactate dehydrogenase activity.", JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY., vol. 53, no. 1, 2005, pages 23 - 34 *
QIN J. ET AL.: "Microfluidic devices for the analysis of apoptosis.", ELECTROPHORESIS., vol. 26, 2005, pages 3780 - 88, XP055097059, DOI: doi:10.1002/elps.200500113 *

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WO2011081434A2 (ko) 2011-07-07

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