WO2013157917A3 - A biosensor and preparation method thereof - Google Patents

A biosensor and preparation method thereof Download PDF

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Publication number
WO2013157917A3
WO2013157917A3 PCT/MY2013/000082 MY2013000082W WO2013157917A3 WO 2013157917 A3 WO2013157917 A3 WO 2013157917A3 MY 2013000082 W MY2013000082 W MY 2013000082W WO 2013157917 A3 WO2013157917 A3 WO 2013157917A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
aflatoxin
sample
detection
sensor
Prior art date
Application number
PCT/MY2013/000082
Other languages
French (fr)
Other versions
WO2013157917A2 (en
Inventor
Kamal Abdul Kadir Mohamad
Salam Faridah
Original Assignee
Institut Penyelidikan Dan Kemajuan Pertanian Malaysia (Mardi)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Penyelidikan Dan Kemajuan Pertanian Malaysia (Mardi) filed Critical Institut Penyelidikan Dan Kemajuan Pertanian Malaysia (Mardi)
Publication of WO2013157917A2 publication Critical patent/WO2013157917A2/en
Publication of WO2013157917A3 publication Critical patent/WO2013157917A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a biological sensor more particularly for detecting aflatoxin B1 in a sample and method of preparation thereof. The advantage of the present invention provides a rapid and efficient detection of aflatoxin B1 in a sample even at high, moderate or low concentration, which is greatly valuable for practical application. Furthermore, the detection of alfatoxin B1 of the present invention requires a simplified pretreatment of sample. Thus, the entire process of sample pre-treatment is simple and fast. Moreover, the detection of aflatoxin B1 by the sensor and the portable reader of the present invention are operated at a simple operation procedure. In another aspect of the present invention, the sensor has produced a high sensitivity. The lowest detectable limit of the aflatoxin B1 in a sample using the sensor produced in the present invention has sensitivity of aflatoxin B1 detection of 1ng/L (part per trillion)
PCT/MY2013/000082 2012-04-20 2013-04-17 A biosensor and preparation method thereof WO2013157917A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2012001755A MY173921A (en) 2012-04-20 2012-04-20 A biosensor and preparation method thereof
MYPI2012001755 2012-04-20

Publications (2)

Publication Number Publication Date
WO2013157917A2 WO2013157917A2 (en) 2013-10-24
WO2013157917A3 true WO2013157917A3 (en) 2013-12-19

Family

ID=49384189

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MY2013/000082 WO2013157917A2 (en) 2012-04-20 2013-04-17 A biosensor and preparation method thereof

Country Status (2)

Country Link
MY (1) MY173921A (en)
WO (1) WO2013157917A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7365330B2 (en) * 2017-07-27 2023-10-19 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Electrically conductive antifouling coating composition
CN108344787B (en) * 2017-12-05 2020-08-28 江苏大学 Preparation method of label-free portable aptamer sensor for detecting AFB1
CN108663505A (en) * 2018-06-08 2018-10-16 中国科学院上海生命科学研究院湖州营养与健康产业创新中心 The remaining kits of aflatoxins M1 and preparation method in a kind of detection dairy produce
CN108845015B (en) * 2018-06-28 2020-09-01 济南大学 Preparation method and application of photoelectrochemical aflatoxin B1 sensor based on tungsten trioxide composite material
CN109187963B (en) * 2018-08-21 2021-11-05 成都师范学院 Preparation and use methods of electrochemical immunosensor for aflatoxin B1 detection
CN109776672A (en) * 2019-02-12 2019-05-21 国家食品安全风险评估中心 Aflatoxin B1 haptens and preparation method thereof, artificial antigen and its application
CN111638326B (en) * 2020-05-20 2023-05-09 江苏大学 Enzyme-free polymer immune probe and preparation method and application thereof
CN111781370A (en) * 2020-05-20 2020-10-16 量准(武汉)生命科技有限公司 Method for measuring molecular binding capacity by utilizing enzyme-labeling instrument and nano-gold particle-enhanced SPR sensor
CN112461803B (en) * 2020-06-12 2023-07-07 重庆工商大学 Method for detecting aflatoxin B1 and ochratoxin A in food sample
CN112179962B (en) * 2020-09-29 2023-06-16 陕西科技大学 Detection method of aflatoxin based on iron ion probe-nano gold/glassy carbon electrode modified electrode
CN112229991B (en) * 2020-10-16 2022-08-16 厦门圣科环保科技股份有限公司 Nano-capture device and preparation method and application thereof
CN112782155B (en) * 2020-12-04 2022-06-28 北京交通大学 Preparation method and application of electrochemiluminescence aflatoxin biosensor
CN113686935B (en) * 2021-08-16 2023-01-31 江西农业大学 Electrochemical sensing detection method and modified electrode for aflatoxin B1 in food
CN113945615B (en) * 2021-10-12 2023-08-01 济南大学 Preparation and application of sandwich ECL sensor based on gold-modified cerium phosphate as efficient coreaction accelerator
CN114436338B (en) * 2022-03-01 2023-07-25 青岛大学 Fe-Mo bimetallic nano-enzyme and preparation method and application thereof
CN114935589A (en) * 2022-03-29 2022-08-23 扬州大学 Electrochemical immunosensor based on mimic enzyme and preparation method and application thereof
CN116381018A (en) * 2023-04-06 2023-07-04 华北理工大学 Modified screen printing electrode, ochratoxin aptamer competitive biosensor and detection method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009510446A (en) * 2005-09-29 2009-03-12 コンビマトリックス・コーポレイション Method and apparatus for measuring binding events on electrode microarrays

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009510446A (en) * 2005-09-29 2009-03-12 コンビマトリックス・コーポレイション Method and apparatus for measuring binding events on electrode microarrays

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GAN NING ET AL.: "A Novel Screen Print Amperommatric Immunosensor to Determine Aflatoxin Bl in Flour Based on Antibody Coated Nano Gold Particles Modified Electrode", JOURNAL OF THE CHINESE CEREALS AND OILS ASSOCIATION, vol. 24, no. 12, 2009, pages 124 - 128 *
YUN TAN ET AL.: "A signal-amplified electrochemical immunosensor for aflatoxin Bl determination in rice", ANALYTICAL BIOCHEMISTRY, vol. 387, pages 82 - 86 *

Also Published As

Publication number Publication date
WO2013157917A2 (en) 2013-10-24
MY173921A (en) 2020-02-27

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