WO2011072354A1 - Circuit pour la collecte d'embryons par voie transcervicale chez les caprins et les ovins - Google Patents

Circuit pour la collecte d'embryons par voie transcervicale chez les caprins et les ovins Download PDF

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Publication number
WO2011072354A1
WO2011072354A1 PCT/BR2010/000415 BR2010000415W WO2011072354A1 WO 2011072354 A1 WO2011072354 A1 WO 2011072354A1 BR 2010000415 W BR2010000415 W BR 2010000415W WO 2011072354 A1 WO2011072354 A1 WO 2011072354A1
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WO
WIPO (PCT)
Prior art keywords
embryo collection
circuit according
embryo
collection circuit
filter
Prior art date
Application number
PCT/BR2010/000415
Other languages
English (en)
Portuguese (pt)
Other versions
WO2011072354A4 (fr
Inventor
Jeferson Ferreira Da Fonseca
José Henrique BRUSCHI
Paulo Mangus Cabral De Barros
Original Assignee
Empresa Brasileira De Pesquisa Agropecuaria - Embrapa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Empresa Brasileira De Pesquisa Agropecuaria - Embrapa filed Critical Empresa Brasileira De Pesquisa Agropecuaria - Embrapa
Publication of WO2011072354A1 publication Critical patent/WO2011072354A1/fr
Publication of WO2011072354A4 publication Critical patent/WO2011072354A4/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation

Definitions

  • the present invention relates to a transcervical embryo collection system developed specifically for small ruminant animals. More particularly, said system is provided with devices that prevent contact with the external environment during process manipulation, protecting embryos and animals (embryo donors) from environmental contamination. Additionally, the present invention further relates to an embryo collection process.
  • bovine species has been the subject of intense research in recent decades. During this period, among other advances, embryo collection and transfer techniques underwent intense changes from totally surgical to totally non-surgical. The advantages of non-surgical procedures are obvious, including animal welfare, decreased life-threatening and surgical sequelae such as hernias and adhesions, among others.
  • Embryo collection in goats and sheep is predominantly performed surgically and laparoscopically with the animals undergoing general anesthesia.
  • both the surgical and the laparoscopic form present all the risks and sequelae inherent to the surgical processes involving the exploration of organs.
  • abdominal organs mainly adhesions.
  • the surgical procedure is not routinely repeated more than three times, since this seems to be the limit of surgical collections, when surgical sequelae, such as adhesions, make new collections unfeasible.
  • WO0152767 describes a device that includes a tube or catheter that is introduced into the animal's vagina into the cervical canal, a flexible tube covered with a coating of also flexible material thus allowing the tube to advance along the cervical canal. and then along the uterine horn after reaching the distal end of the tube.
  • the device shown prevents the absorption of reflux when it is transported out.
  • FR2607011 discloses a device that can be used for transcervical or peritoneal transfer of embryos.
  • the device presented consists of a piston syringe, a device already known for use in artificial insemination, and not susceptible for use in embryo collection, especially goats and sheep, due to its morphological peculiarities.
  • embryo transfer refers to the act of innovation. Innovation is the act of depositing in the female uterus (recipient) an embryo previously collected from another female (donor).
  • DE3730347 describes an embryo collection instrument for collecting embryos from small ruminants such as goats and sheep.
  • the device has holes in the end and uses fluids to promote embryo suspension.
  • the embryo collection described is made through the laparoscopy method.
  • the related technique further comprises several other patent documents, as well as scientific articles describing methods of embryo collection via the transcervical approach.
  • the described methods are surgical methods that present the need for improvement to optimize the process and to promote better conditions of its accomplishment.
  • Such aspects may reflect in improved welfare conditions. during the process, as well as reducing risks and surgical sequelae, allowing the use of animals for a relatively longer period.
  • it may provide additional consumption and export of the product.
  • the present invention has been developed in order to provide the technique of a system comprising devices with measures suitable for goats and sheep in particular, by double flow and closed loop.
  • the present invention relates to a transcervical embryo collection system developed specifically for small ruminant animals. More particularly, said system is provided with devices that prevent contact with the external environment during process manipulation, protecting the embryos from environmental contamination, as well as a device provided with a double flow path, to allow the change of direction of the embryo. Uterine infusion of the culture medium with a simple touch. Specifically, the function of the developed technology is to sequentially and sequentially carry the culture medium flush fluid into the female's uterus, allowing the uterine content of females subjected to superovulation and embryo collection to be completely enclosed. . Additionally, the present invention further relates to a process for collecting embryos.
  • Figure 1 shows the embryo collection system object of the present embodiment.
  • Figure 2 shows the connecting device (1) and flow restrictors (2).
  • FIG. 3 shows the filter system of the invention.
  • Figure 4 shows the flow restricting device assembly
  • Figure 5 shows the system connecting system with the uterine lavage pouch used in the system of the present invention.
  • the present invention relates to a transcervical embryo collection system developed specifically for small ruminant animals such as goats and sheep, taking into account the anatomical peculiarities of the species.
  • the present invention is not limited to the use of said system in goats and sheep and said system can be used on any animal of similar size.
  • said system has great efficiency in embryo collection and is provided with devices that prevent contact with the external environment during process manipulation, protecting the embryos from environmental contamination.
  • a second particular aspect of the present invention is that said system comprises a device provided with a double flow path, to allow the uterine infusion of the culture medium to be changed with a single touch. This feature represents a great facilitation in the embryo collection process.
  • the embryo collection system is also referred to by experts in the art as the embryo collection circuit, and especially the present system will be known by such specialists as embryo collection loop.
  • the elements that make up the present system are made of a material with specificity suitable for the small volume used for the type of animal for which it is intended, for example, for goats and sheep, maximizing the return flow of fluid from inside the womb.
  • Said material may be sterilized by cobalt 60 gamma radiation and / or by ethylene oxide gas, being a non-toxic and pyrogen-free material.
  • the entire system is comprised of plastics material, preferably but not limited to, for example, vinyl polychloride - PVC - ATP-109 CRISTAL.
  • plastics material preferably but not limited to, for example, vinyl polychloride - PVC - ATP-109 CRISTAL.
  • the characteristic of the system related to the use of a smaller amount of plastic material makes the developed system less polluting to the environment.
  • the function of the developed technology is to carry the flushing fluid with culture medium in the bag, to the female's uterus, sequentially and successively, allowing the uterine content of the females submitted to superovulation and embryo collection.
  • the washing liquid used as culture medium was a phosphate buffered saline solution - PBS.
  • any other solution that has the same characteristics as the PBS solution may be used as a wash solution for the system now developed, provided they meet the requirements for embryo collection.
  • the embryo collection system (1) now developed is fully shown in Figure 1 and comprises:
  • FIG. 1 shows the complete system with all its elements.
  • the collection device (1) of the embryo collection system is shown in Figure 2.
  • the connector device for the collection probe (1) and the flow control devices (2) can be seen.
  • Such a device has a Y-shape and has different elements connected to each of the 3 system outputs.
  • the collecting device (1) formed by a connector for the collection probe.
  • Figure 2 shows this outlet at which the flow restricting device (5) is coupled to the connector of the embryo collection probe (1) that passes through the cervix and is positioned within the uterus.
  • Said flow restricting device (5) has a Y-shaped structural configuration, allowing both the flow of liquid into the uterine interior and the flow of liquid to the filter. For its sequential total locking a pulley is turned inside.
  • the preferred distance of the location of said device (5) between the connector (1) and the embryo collection filter (3) is approximately 80cm.
  • the filter device for embryo collection (3) and its corresponding seal (4) housed in a ventral manner.
  • the flow restricting device (2) is positioned. This set constitutes the second output end of the system.
  • the filter which is coupled to the flow restricting system (2), allows the passage of liquid and the retention of embryos. As the volume inside the filter increases, the filter device allows controlled disposal while maintaining a safe amount of liquid inside, preventing overfilling or drying.
  • the filters of the invention are shown in detail in Figure 3.
  • tap (5) is coupled to a sterile dosing device (not shown in the figures), such as a syringe, which allows a perfect dosage of the amount of liquid to be used in the embryo collection procedure without the need to open the system (Figure 1) external medium.
  • a sterile dosing device such as a syringe
  • such a metering device holds approximately 60 mL.
  • devices of other capacities may be easily adapted to the system according to its dimensions.
  • the connector device After the flow controller is the connector device with the uterine lavage bag.
  • Said device consists of a connector (7) and a seal (8), which internally engage forming the third end of the system (Figure 1) which is connected to a bag containing the wash solution, not shown in the accompanying Figures. present report.
  • Figure 5 shows details of the connection system to the uterine lavage bag.
  • the embryo collection process developed by the present invention basically comprises: (i) aspirating the culture medium into the syringe; (ii) reversing a tap; (iii) injecting the culture medium into the female's uterus in volumes of approximately 10 to 30 mL, more preferably 15 mL sequentially, making 240 mL per uterine horn.
  • the flow restricting device pathway that carries the fluid into the uterus is closed, allowing gravity to return the uterine content through the other pathway of the uterine content device to the uterus. filter. Subsequently, it is again opened for a new injection procedure and so on.
  • the distance measurements between the devices comprising the present system developed herein are exemplary values of the present invention, and are not limited thereto to such values, and said system can be sized according to the characteristics of the present invention.
  • the present invention provides a suitable embryo collection system for small ruminant animals, such as goats and sheep, with dual loop and closed loop, reducing contact with the external environment and avoiding contamination.
  • the present system is a product that adds functionality to anatomical concepts specific to sheep and goats. Additionally, the product is cheaper than similar bovine because it uses less fluid to perform uterine lavage, is more efficient because it allows greater retrograde flow of this fluid and is more aseptic because it allows collection in a completely enclosed environment.
  • the process for embryo collection comprises:
  • the amount of PBS buffered saline may vary depending on the size of the ruminant to be used and is well known to those skilled in the art.

Abstract

La présente invention concerne un système de collecte d'embryons par voie transcervicale, mis au point spécifiquement pour les animaux ruminants de petite taille. Plus particulièrement, ledit système comprend des dispositifs qui empêchent le contact avec le milieu extérieur pendant la réalisation du procédé, de manière à protéger les embryons et les animaux (donneurs d'embryons) contre une contamination extérieure, ainsi qu'un dispositif à double voie d'écoulement permettant de changer le sens de la perfusion utérine au moyen d'un simple toucher. L'invention se rapporte en outre à un procédé de collecte d'embryons.
PCT/BR2010/000415 2009-12-17 2010-12-15 Circuit pour la collecte d'embryons par voie transcervicale chez les caprins et les ovins WO2011072354A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRPI0905581-9A BRPI0905581B1 (pt) 2009-12-17 2009-12-17 Circuito para coleta de embriões pela via transcervical em caprinos e ovinos
BRPI0905581-9 2009-12-17

Publications (2)

Publication Number Publication Date
WO2011072354A1 true WO2011072354A1 (fr) 2011-06-23
WO2011072354A4 WO2011072354A4 (fr) 2011-08-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BR2010/000415 WO2011072354A1 (fr) 2009-12-17 2010-12-15 Circuit pour la collecte d'embryons par voie transcervicale chez les caprins et les ovins

Country Status (2)

Country Link
BR (1) BRPI0905581B1 (fr)
WO (1) WO2011072354A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012174658A1 (fr) * 2011-06-21 2012-12-27 Gavaga Quinn A Transfert d'embryons
CN117598764A (zh) * 2024-01-24 2024-02-27 内蒙古医科大学附属医院(内蒙古自治区心血管研究所) 一种注射体积可控的胚胎子宫移植管

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE7615524U1 (de) * 1976-05-15 1976-10-21 Semm, Kurt, Prof. Dr.Med., 2300 Kiel Insufflations-nadel fuer die erzeugung einer z.b. gasgefuellten hoehle im bauchraum
GB1583804A (en) * 1976-07-13 1981-02-04 Cassou B Injection gun for animals in particular for the artificial insemination of cattle
DE8104329U1 (de) * 1981-07-30 Richard Wolf Gmbh, 7134 Knittlingen Endoskop, insbesondere Laparoskop
DE8322900U1 (de) * 1983-12-29 Richard Wolf Gmbh, 7134 Knittlingen Vorrichtung zur Zertrümmerung von Steinen in Körperhöhlen
US4607619A (en) * 1984-02-28 1986-08-26 Snow Brand Milk Products, Co., Ltd. Locking mechanism for an endoscope
BR8603951A (pt) * 1985-08-19 1987-03-24 Agronomique Inst Nat Rech Instrumento para inseminacao artificial,o transplante de embrioes in-utero ou a retirada de liquido folicular por via transperitoneal nos animais
US4653475A (en) * 1984-02-28 1987-03-31 Snow Brand Milk Products Co., Ltd. Embryo transferring apparatus adapted for endoscope
US4700694A (en) * 1984-02-20 1987-10-20 Olympus Optical Co., Ltd. Endoscope means and ovum picker employed by inserting through endoscope means
FR2603796A1 (fr) * 1986-09-17 1988-03-18 Agronomique Inst Nat Rech Instrumentation pour la collecte d'embryons, sous laparoscopie, chez les petits ruminants
US4781706A (en) * 1986-05-14 1988-11-01 Nissho Corporation Device for collecting and selecting embryos
JPH01113073A (ja) * 1987-10-28 1989-05-01 Olympus Optical Co Ltd 配偶子移植カテーテル
EP0478155A1 (fr) * 1990-09-28 1992-04-01 Ovamed Corporation Cathéter d'injection
US5505716A (en) * 1993-02-02 1996-04-09 Simmet; Ludwig O. Embryo collector

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE8104329U1 (de) * 1981-07-30 Richard Wolf Gmbh, 7134 Knittlingen Endoskop, insbesondere Laparoskop
DE8322900U1 (de) * 1983-12-29 Richard Wolf Gmbh, 7134 Knittlingen Vorrichtung zur Zertrümmerung von Steinen in Körperhöhlen
DE7615524U1 (de) * 1976-05-15 1976-10-21 Semm, Kurt, Prof. Dr.Med., 2300 Kiel Insufflations-nadel fuer die erzeugung einer z.b. gasgefuellten hoehle im bauchraum
GB1583804A (en) * 1976-07-13 1981-02-04 Cassou B Injection gun for animals in particular for the artificial insemination of cattle
US4700694A (en) * 1984-02-20 1987-10-20 Olympus Optical Co., Ltd. Endoscope means and ovum picker employed by inserting through endoscope means
US4653475A (en) * 1984-02-28 1987-03-31 Snow Brand Milk Products Co., Ltd. Embryo transferring apparatus adapted for endoscope
US4607619A (en) * 1984-02-28 1986-08-26 Snow Brand Milk Products, Co., Ltd. Locking mechanism for an endoscope
BR8603951A (pt) * 1985-08-19 1987-03-24 Agronomique Inst Nat Rech Instrumento para inseminacao artificial,o transplante de embrioes in-utero ou a retirada de liquido folicular por via transperitoneal nos animais
US4781706A (en) * 1986-05-14 1988-11-01 Nissho Corporation Device for collecting and selecting embryos
FR2603796A1 (fr) * 1986-09-17 1988-03-18 Agronomique Inst Nat Rech Instrumentation pour la collecte d'embryons, sous laparoscopie, chez les petits ruminants
JPH01113073A (ja) * 1987-10-28 1989-05-01 Olympus Optical Co Ltd 配偶子移植カテーテル
EP0478155A1 (fr) * 1990-09-28 1992-04-01 Ovamed Corporation Cathéter d'injection
US5505716A (en) * 1993-02-02 1996-04-09 Simmet; Ludwig O. Embryo collector

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012174658A1 (fr) * 2011-06-21 2012-12-27 Gavaga Quinn A Transfert d'embryons
CN117598764A (zh) * 2024-01-24 2024-02-27 内蒙古医科大学附属医院(内蒙古自治区心血管研究所) 一种注射体积可控的胚胎子宫移植管
CN117598764B (zh) * 2024-01-24 2024-03-19 内蒙古医科大学附属医院(内蒙古自治区心血管研究所) 一种注射体积可控的胚胎子宫移植管

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Publication number Publication date
BRPI0905581A2 (pt) 2011-08-16
BRPI0905581B1 (pt) 2021-03-23
WO2011072354A4 (fr) 2011-08-11

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