WO2011067439A3 - Bacterial strain cect7623, uses thereof and xeroprotectant product produced from same - Google Patents

Bacterial strain cect7623, uses thereof and xeroprotectant product produced from same Download PDF

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Publication number
WO2011067439A3
WO2011067439A3 PCT/ES2010/000515 ES2010000515W WO2011067439A3 WO 2011067439 A3 WO2011067439 A3 WO 2011067439A3 ES 2010000515 W ES2010000515 W ES 2010000515W WO 2011067439 A3 WO2011067439 A3 WO 2011067439A3
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WO
WIPO (PCT)
Prior art keywords
xeroprotectant
composition
cect7623
relates
microorganism
Prior art date
Application number
PCT/ES2010/000515
Other languages
Spanish (es)
French (fr)
Other versions
WO2011067439A2 (en
Inventor
Maximino Manzanera Ruiz
Jesús Juan GONZÁLEZ LÓPEZ
Juan Jesús NARVÁEZ REINALDO
Lucía SANTA CRUZ CALVO
Original Assignee
Universidad De Granada
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Publication date
Application filed by Universidad De Granada filed Critical Universidad De Granada
Publication of WO2011067439A2 publication Critical patent/WO2011067439A2/en
Publication of WO2011067439A3 publication Critical patent/WO2011067439A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/04—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/06—Arthrobacter

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Storage Of Fruits Or Vegetables (AREA)

Abstract

The invention relates to a microorganism of the Arthrobacter sp. species (access number CECT7623). The invention also relates to the use of said microorganism or a population of same for the production of a xeroprotectant composition, said composition comprising b-hydroxybutyrate, glucuronic acid, glutamic acid, glutamine and glucose or comprising glutamine, glucose and trehalose. The invention further relates to the use of the xeroprotectant composition for preserving biological material with a residual moisture content equal to or less than 10%, in which the biological material is an invertebrate organism, a seed, a plantlet, a microorganism, an isolated organ, an isolated biological tissue, a cell or a molecule with biological activity such as, for example, an enzyme with lipase activity. Furthermore, the invention relates to a method for obtaining the xeroprotectant composition or a method for preserving said biological material.
PCT/ES2010/000515 2009-12-04 2010-12-03 Bacterial strain cect7623, uses thereof and xeroprotectant product produced from same WO2011067439A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES200931116A ES2362026B2 (en) 2009-12-04 2009-12-04 BACTERIAN CEPA CECT7623, USES AND XEROPROTECTOR PRODUCT PRODUCED BY THE SAME.
ESP200931116 2009-12-04

Publications (2)

Publication Number Publication Date
WO2011067439A2 WO2011067439A2 (en) 2011-06-09
WO2011067439A3 true WO2011067439A3 (en) 2011-11-17

Family

ID=44115358

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2010/000515 WO2011067439A2 (en) 2009-12-04 2010-12-03 Bacterial strain cect7623, uses thereof and xeroprotectant product produced from same

Country Status (2)

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ES (3) ES2381289B2 (en)
WO (1) WO2011067439A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA06014490A (en) * 2006-12-13 2008-10-10 Gabriel Iturriaga De La Fuente Method for improving the yield and tolerance to abiotic stress in leguminous and grass inoculated with bacteria having and increased trehalose content.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA06014490A (en) * 2006-12-13 2008-10-10 Gabriel Iturriaga De La Fuente Method for improving the yield and tolerance to abiotic stress in leguminous and grass inoculated with bacteria having and increased trehalose content.

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BOYLEN, C.W.: "Survival of Arthrobacter crystallopoietes during prolonged periods of extreme desiccation", JOURNAL OF BACTERIOLOGY, vol. 113, no. 1, 1973, pages 33 - 37 *
CAI Z. ET AL.: "Trehalose-6-phosphate synthase 1 from Metarhizium anisopliae: clone, expression and properties of the recombinant", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 107, no. 5, 22 April 2009 (2009-04-22), pages 499 - 505, XP026020546, DOI: doi:10.1016/j.jbiosc.2009.01.007 *
LI A. ET AL.: "Ethylene response factor TERF1 enhances glucose sensitivity in tobacco through activating the expression of sugar-related genes", JOURNAL OF INTEGRATIVE PLANT BIOLOGY, vol. 51, no. 2, 2008, pages 184 - 193 *
MONGODIN, E.F.: "Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TCl", PLOS GENETICS, vol. 2, no. 12, 2006, pages 2094 - 2106, XP002454726, DOI: doi:10.1371/journal.pgen.0020214 *
NARVAEZ-REINALDO, J.J. ET AL.: "Rapid method for isolation of desiccation-tolerant strains and xeroprotectants", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 76, no. 15, August 2010 (2010-08-01), pages 5254 - 5262 *
OVERHAGE, J.: "Identification of large linear plasmids in Arthrobacter spp. encoding the degradation of quinaldine to anthranilate", MICROBIOLOGY, vol. 151, 2005, pages 491 - 500 *

Also Published As

Publication number Publication date
ES2381289B2 (en) 2012-12-10
ES2381660A1 (en) 2012-05-30
ES2381289A1 (en) 2012-05-24
ES2362026B2 (en) 2011-11-15
WO2011067439A2 (en) 2011-06-09
ES2362026A1 (en) 2011-06-27
ES2381660B2 (en) 2012-12-10

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