WO2011066497A3 - Procédés et appareil destinés à la ségrégation de particules, y compris la ségrégation et la prolifération de cellules foetales et souches - Google Patents

Procédés et appareil destinés à la ségrégation de particules, y compris la ségrégation et la prolifération de cellules foetales et souches Download PDF

Info

Publication number
WO2011066497A3
WO2011066497A3 PCT/US2010/058172 US2010058172W WO2011066497A3 WO 2011066497 A3 WO2011066497 A3 WO 2011066497A3 US 2010058172 W US2010058172 W US 2010058172W WO 2011066497 A3 WO2011066497 A3 WO 2011066497A3
Authority
WO
WIPO (PCT)
Prior art keywords
particles
segregation
fetal
proliferation
methods
Prior art date
Application number
PCT/US2010/058172
Other languages
English (en)
Other versions
WO2011066497A2 (fr
Inventor
George Hvichia
David Counts
Gary Evans
Original Assignee
Parsortix, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Parsortix, Inc. filed Critical Parsortix, Inc.
Priority to BR112012013034A priority Critical patent/BR112012013034A2/pt
Priority to CN201080060399.2A priority patent/CN102695804B/zh
Priority to MX2012006244A priority patent/MX2012006244A/es
Priority to EP10834005.0A priority patent/EP2507382A4/fr
Priority to CA2782176A priority patent/CA2782176C/fr
Priority to JP2012541219A priority patent/JP2013511992A/ja
Publication of WO2011066497A2 publication Critical patent/WO2011066497A2/fr
Publication of WO2011066497A3 publication Critical patent/WO2011066497A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0272—Investigating particle size or size distribution with screening; with classification by filtering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dispersion Chemistry (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Ecology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un appareil destiné à la ségrégation de particules en se basant sur leur capacité à circuler à travers une voie de passage étagée. Au moins une partie des particules sont disposées dans un passage lié à un premier étage, mais au moins une partie des particules ne sont pas capables de passer à travers un passage plus petit lié à un second étage, résultant en la ségrégation des particules. Les appareils et procédés décrits ici peuvent être utilisés dans la ségrégation de particules d'une grande variété de types. A titre d'exemple, ils peuvent être utilisés dans la ségrégation de cellules de type fœtales provenant d'un échantillon de sang maternel tel que du sang artériel maternel.
PCT/US2010/058172 2009-11-30 2010-11-29 Procédés et appareil destinés à la ségrégation de particules, y compris la ségrégation et la prolifération de cellules fœtales et souches WO2011066497A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
BR112012013034A BR112012013034A2 (pt) 2009-11-30 2010-11-29 métodos e aparelhos para segregação de partículas, incluindo a segregação e a proliferação de células fetais e células-tronco
CN201080060399.2A CN102695804B (zh) 2009-11-30 2010-11-29 用于分离颗粒的方法和设备，包括分离和增殖胚胎及干细胞
MX2012006244A MX2012006244A (es) 2009-11-30 2010-11-29 Metodos y aparatos para segregacion de particulas, incluyendo segregacion y proliferacion de celulas fetales y celulas madre.
EP10834005.0A EP2507382A4 (fr) 2009-11-30 2010-11-29 Procédés et appareil destinés à la ségrégation de particules, y compris la ségrégation et la prolifération de cellules fétales et souches
CA2782176A CA2782176C (fr) 2009-11-30 2010-11-29 Procedes et appareil destines a la segregation de particules, y compris la segregation et la proliferation de cellules foetales et souches
JP2012541219A JP2013511992A (ja) 2009-11-30 2010-11-29 胎児細胞および幹細胞の分離および増殖を含む粒子の分離のための方法および装置

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26491809P 2009-11-30 2009-11-30
US61/264,918 2009-11-30

Publications (2)

Publication Number Publication Date
WO2011066497A2 WO2011066497A2 (fr) 2011-06-03
WO2011066497A3 true WO2011066497A3 (fr) 2011-11-17

Family

ID=43332885

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/058172 WO2011066497A2 (fr) 2009-11-30 2010-11-29 Procédés et appareil destinés à la ségrégation de particules, y compris la ségrégation et la prolifération de cellules fœtales et souches

Country Status (8)

Country Link
EP (1) EP2507382A4 (fr)
JP (2) JP2013511992A (fr)
CN (1) CN102695804B (fr)
AU (1) AU2010246381B2 (fr)
BR (1) BR112012013034A2 (fr)
CA (1) CA2782176C (fr)
MX (1) MX2012006244A (fr)
WO (1) WO2011066497A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2587248A1 (fr) * 2011-10-25 2013-05-01 Koninklijke Philips Electronics N.V. Filtrage de particules à partir de sang ou autres supports
US9631179B2 (en) 2013-03-15 2017-04-25 Angle North America, Inc. Methods for segregating particles using an apparatus with a size-discriminating separation element having an elongate leading edge
EP3212331B1 (fr) 2014-10-27 2021-06-23 Board of Regents, The University of Texas System Plate-forme d'imagerie à haut débit
CN105176812B (zh) * 2015-07-28 2017-10-03 浙江奥瑞健生物技术有限公司 一种干细胞球切割收集装置及干细胞球的切割分离传代方法
US10946381B2 (en) * 2015-08-18 2021-03-16 National Health Research Institutes Microfluidic hydrodynamic shuttling chip device for highthroughput multiple single cells capture
GB201704766D0 (en) * 2017-01-05 2017-05-10 Illumia Inc System and methods for selective effluent collection
EP3561038A1 (fr) * 2018-04-24 2019-10-30 Bayer AG Séparateur de cellules
US11389799B2 (en) * 2019-01-17 2022-07-19 The Regents Of The University Of Michigan Microfluidic device for size and deformability measurements and applications thereof
CN113454437B (zh) * 2019-02-27 2024-03-15 京瓷株式会社 粒子分离器件以及粒子分离计测装置
CN109810894B (zh) * 2019-03-01 2022-06-10 长春理工大学 一种基于光诱导介电泳的群细胞三维结构操纵与构建系统及方法
JP7502740B2 (ja) 2019-07-23 2024-06-19 国立大学法人佐賀大学 剪断処理による微細藻類の外被破壊法及び外被破壊システム

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6008040A (en) * 1995-07-07 1999-12-28 Synosys, Inc. Procedures for efficient separation of cells, cellular materials and proteins
US20070072290A1 (en) * 2001-07-17 2007-03-29 Georgi Hvichia Microstructure for particle and cell separation, identification, sorting, and manipulation
US20070172903A1 (en) * 2002-09-27 2007-07-26 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
WO2009131645A2 (fr) * 2008-04-23 2009-10-29 Parsortix, Inc. Procédés et appareil pour la ségrégation de particules

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6783928B2 (en) * 2001-07-17 2004-08-31 Georgi Hvichia Microstructures for cell proliferation assays and semen analysis
US20070039463A1 (en) * 2003-10-02 2007-02-22 Gert Desmet Method and device for size-separating particles present in a fluid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6008040A (en) * 1995-07-07 1999-12-28 Synosys, Inc. Procedures for efficient separation of cells, cellular materials and proteins
US20070072290A1 (en) * 2001-07-17 2007-03-29 Georgi Hvichia Microstructure for particle and cell separation, identification, sorting, and manipulation
US20070172903A1 (en) * 2002-09-27 2007-07-26 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
WO2009131645A2 (fr) * 2008-04-23 2009-10-29 Parsortix, Inc. Procédés et appareil pour la ségrégation de particules

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
See also references of EP2507382A4 *
WEGNER CC. ET AL.: "Human parvovirus B19 VP2 empty capsids bind to human vil lous trophoblast cells in vitro via the globoside receptor.", INFECT. DIS. OBST ET. GYNECOL., vol. 12, no. 2, 2004, pages 69 - 78, XP008158889 *

Also Published As

Publication number Publication date
CN102695804A (zh) 2012-09-26
JP2013511992A (ja) 2013-04-11
MX2012006244A (es) 2012-10-15
CA2782176A1 (fr) 2011-06-03
CA2782176C (fr) 2015-10-06
EP2507382A2 (fr) 2012-10-10
AU2010246381B2 (en) 2013-08-15
CN102695804B (zh) 2014-09-03
WO2011066497A2 (fr) 2011-06-03
AU2010246381A1 (en) 2010-12-16
JP2016208978A (ja) 2016-12-15
BR112012013034A2 (pt) 2016-10-04
EP2507382A4 (fr) 2016-06-08

Similar Documents

Publication Publication Date Title
WO2011066497A3 (fr) Procédés et appareil destinés à la ségrégation de particules, y compris la ségrégation et la prolifération de cellules foetales et souches
WO2009131645A3 (fr) Procédés et appareil pour la ségrégation de particules
WO2008014516A3 (fr) Sélection de cellules au moyen de biomarqueurs
WO2014152048A3 (fr) Ensembles et procédés de réduction de la diaphonie optique dans les systèmes de traitement des particules
WO2008039918A3 (fr) Procédés et appareils pour la détection de contours hybrides
MX356042B (es) Métodos para segregar partículas usando un aparato con un elemento de separación por discriminación de tamaño que tiene un borde delantero alargado.
WO2012051996A3 (fr) Procédé et dispositif pour mesurer et éliminer des variations du système dans un dispositif de traitement du sang
WO2010027446A3 (fr) Essais et systèmes de cyclage de vésicules synaptiques
EP2006016A4 (fr) Dispositif de separation par diffusion poreuse a film plat, dispositif de condensation a film plat, film poreux en cellulose regeneree pour diffusion poreuse et procede de controle non destructif de film plat
WO2011094572A3 (fr) Dispositifs, systèmes et/ou procédés pour cultures en gouttes suspendues
WO2012110878A3 (fr) Méthode de diagnostic du cancer et trousse de diagnostic à l'aide de la mesure de l'activité des cellules nk
WO2009076149A3 (fr) Systèmes et procédés d'utilisation de résultats de simulation de flux basés sur des cellules pour calculer des trajectoires aérodynamiques
WO2008084409A3 (fr) Moyen d'identification d'une souche isolée à partir d'un échantillon clinique au niveau de l'espèce et/ou de la sous-espèce
EA201390638A1 (ru) Обогащение и идентификация фетальных клеток в материнской крови и лиганды для такого применения
WO2007136980A3 (fr) Support de test de diagnostic et procédés pour sa fabrication
WO2012033537A8 (fr) Références pour l'identification des cellules normales
WO2007003801A3 (fr) Methode et systeme pour la mesure et l'etude de l'encrassement d'un reacteur
WO2010039802A3 (fr) Procédés et compositions pour isoler un acide nucléique
GB0711327D0 (en) Diagnostic method
WO2008073627A3 (fr) Procédé de diagnostic et de traitement de l'asthme
WO2012106474A3 (fr) Procédés et systèmes pour évaluer l'intégrité d'une cavité utérine
WO2010078872A3 (fr) Enrichissement et identification de cellules foetales dans le sang maternel et ligands destinés à y être utilisés
WO2008136853A3 (fr) Procédés de séparation de nanoparticules magnétiques
NZ608316A (en) Methods and compositions for diagnosis and prognosis of renal injury and renal failure
WO2010081640A3 (fr) Procédé et dispositif destinés à séparer de l'argon d'un mélange gazeux

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10834005

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2012541219

Country of ref document: JP

ENP Entry into the national phase

Ref document number: 2782176

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 4712/CHENP/2012

Country of ref document: IN

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: MX/A/2012/006244

Country of ref document: MX

REEP Request for entry into the european phase

Ref document number: 2010834005

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2010834005

Country of ref document: EP

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112012013034

Country of ref document: BR

ENP Entry into the national phase

Ref document number: 112012013034

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20120530