WO2011060926A1 - Dispositif et procédé de culture d'organismes - Google Patents

Dispositif et procédé de culture d'organismes Download PDF

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Publication number
WO2011060926A1
WO2011060926A1 PCT/EP2010/007000 EP2010007000W WO2011060926A1 WO 2011060926 A1 WO2011060926 A1 WO 2011060926A1 EP 2010007000 W EP2010007000 W EP 2010007000W WO 2011060926 A1 WO2011060926 A1 WO 2011060926A1
Authority
WO
WIPO (PCT)
Prior art keywords
medium
agitator
gas
light
drive shaft
Prior art date
Application number
PCT/EP2010/007000
Other languages
German (de)
English (en)
Inventor
Hilmar Franke
Rafael Meinhardt
Anneliese Niederl-Schmidinger
Karl Stagl
Original Assignee
Universität Duisburg-Essen
See-O-Two Patentgesellschaft Mbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP20100000900 external-priority patent/EP2325293B1/fr
Application filed by Universität Duisburg-Essen, See-O-Two Patentgesellschaft Mbh filed Critical Universität Duisburg-Essen
Priority to DE112010004498T priority Critical patent/DE112010004498A5/de
Publication of WO2011060926A1 publication Critical patent/WO2011060926A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C12M29/08—Air lift
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor

Definitions

  • the present invention relates to an arrangement according to the preamble of claim 1 and a method according to the preamble of claim 12.
  • the present invention generally relates to the cultivation of organisms.
  • the present invention relates to the production of biomass or of metabolic products of preferably photosynthesizing organisms, in particular for the conversion of carbon dioxide into biomass, wherein a liquid medium comprising the organisms, in particular a liquid medium with algae, illuminates and a gas, in particular carbon dioxide-containing or consisting of gas, is introduced into the medium to stimulate the growth and / or proliferation of the organisms and to form biomass or metabolic products.
  • the present invention is particularly concerned with mimicking photosynthesis for binding carbon dioxide. It is known to illuminate an algae suspension for this purpose in order to stimulate the growth of the algae.
  • the algae consume carbon dioxide for growth and biomass production.
  • the algae suspension can be circulated and gas can be introduced.
  • WO 2008/135276 A2 an arrangement and a method for producing biomass are known, wherein an algal suspension can be circulated by means of a stirrer or the like in a container and carbon dioxide-containing gas is introduced at the bottom or through the bottom of the container in the algal suspension , WO 2008/135276 A2 gives no indication for the realization of the agitator.
  • the present invention has for its object to provide an arrangement and a method available, wherein a simple structure, a simple, inexpensive and / or energy-saving process flow and / or high efficiency is or will be possible.
  • a basic idea of the present invention is to introduce gas through the agitator or its drive shaft or along an axis of rotation of the agitator into the container or into a liquid medium which preferably contains organisms, in particular algae. This allows a combination of the gas inlet and the agitator and allows a structural simplification. Furthermore, this easily allows the generation of a bubble chain from the gas in the medium.
  • the drive shaft of the agitator or a rotor of the agitator for the introduction of gas is at least partially hollow. This allows a very simple implementation.
  • the gas supply is preferably equipped with a check valve, in particular on the stirrer or on the drive shaft, in order to prevent undesired penetration of the medium or of water or the like when the gas supply is shut off.
  • the check valve is arranged on the projecting into the container end of the drive shaft.
  • a contactless, uniform and / or complete illumination of the medium, which penetrates deep into the medium can be achieved. If light is coupled into the bubble chain, it acts like a light guide, wherein a high light intensity in the bubble chain over the surface of the bubbles in the medium and the radiated intensity due to multiple reflection can be greatly attenuated, which is beneficial to metabolic activity and growth of organisms effect.
  • the gas is simultaneously introduced into the medium via the bubble chain.
  • bubble chain is intended to express that gas bubbles at least substantially linearly rise in a row in a row or even connect to each other.
  • bubble chain in a further meaning preferably also be understood as a bubble vortex or bubble cloud.
  • sunlight is used to illuminate the medium, even if basically artificially generated light can be used.
  • the medium is irradiated with non-visible electromagnetic radiation, for example with infrared or ultraviolet radiation.
  • illumination and light therefore preferably also broadly include irradiation with or initiation of other electromagnetic radiation.
  • a single-row bubble chain is created, which is formed by a plurality of substantially equal or different sized in a row consecutive bubbles.
  • the bubble chain it is also possible for the bubble chain to be formed by a multiplicity of bubbles of the same or different size, which rise upwards in several rows next to one another and form a tubular, bubble-filled light guide space.
  • the adjacent bubbles of the bubble chain the light within the medium can be distributed in a simple manner.
  • the agitator generates a circular or spiral movement of the medium, whereby a bubble chain extending in a straight line along an axis of rotation of the medium can be formed.
  • the gas bubbles collect in the region of the axis of rotation and form a bubble chain.
  • the shape and size of the bubble chain depends, inter alia, on the angular velocity of the moving medium.
  • a bubble chain can in principle, however, also be formed in non-circular or spiral-shaped media.
  • the bubble chain produced can extend at least substantially continuously or without interruption from the gas supply in the bottom region of the medium up to the surface of the medium. This allows in a simple manner lighting or lighting of the bubble chain, preferably from above, but if necessary also from the side or from below. The light is then distributed over the bubble chain as a light guide in the medium. Depending on the flow conditions in the container, the invention also allows individual bubbles of the bubble chain to separate from one another for a short time, so that the bubble chain is interrupted at least temporarily.
  • bubble chain is to be understood in a preferred sense also generally to the effect that it is an optically permeable, in particular elongated space area or gas area in the medium.
  • the illumination device can be arranged at a sufficient distance from the medium, wherein, for example, an illumination of the bubble chain can be provided from above into the exit of the gas bubbles from the medium.
  • lighting may also be provided through the side walls and / or from below into the bubble chain. In the case of illumination from below and from above, it is possible in particular to realize a greater container or media height, since the light can penetrate from opposite sides into the medium.
  • the contactless illumination of the gas bubbles is particularly advantageous if the medium contains algae or other organisms that grow very easily on surfaces that come into contact with the medium.
  • the growth of algae on transparent irradiated surfaces of the illumination device can lead to a decrease in transparency and reduced illumination performance after a short time.
  • the illumination of the bubble chain can take place at least substantially in the vertical direction, for example starting from an area above the medium, the light being directed onto the exit area of the bubble chain on the surface of the medium. Basically, it is synonymous possible that a lighting of the bubble chain is made obliquely from above or from the side of the medium.
  • a detection device for determining or tracking the exit region of the bubble chain can be provided on the surface of the medium.
  • the detection device can have means for optically determining and tracking the exit region.
  • the detection device can detect the size and / or the shape and / or the circumferential profile of the exit region and / or the occurrence of a plurality of exit regions, so that it is easy to draw conclusions about the position and course of the bubble chain in the medium or
  • the shape and size of the bubble chain are possible.
  • the detection device can be designed as an alternative or in addition to the direct determination of the position and / or the course of the bubble chain in the medium or for determining the shape and size of the bubble chain.
  • a corresponding, preferably optical detection system can be provided.
  • the illumination device or the light can be directed onto the bubble chain in order to ensure illumination of the bubble chain, in particular even with a moving bubble chain in the medium.
  • the illumination device can preferably be automatically aligned with the exit region of the bubble chain, so that the light strikes the exit region, for example, even when the exit region of the gas bubbles migrates on the surface.
  • a measuring device may additionally or alternatively be provided for the detection device.
  • a control device for controlling the orientation of the illumination device and / or for controlling the device for generating a movement of the medium depending on the position of the exit region of the bubble chain on the surface of the medium and / or the position and / or the size the bubble chain can be provided in the medium and / or the flow profile and / or the flow velocity of the medium.
  • the shape, size and position of the bubble chain can be influenced in a simple manner by changing the flow velocity and / or the flow profile produced.
  • FIG. 1 shows a schematic representation, not to scale, of a proposed arrangement 1. This serves for the cultivation of organisms or the introduction of gas 6 into a liquid medium 3 and in particular the three-dimensional distribution of light 2 in the medium 3.
  • the medium 3 is in particular a suspension or the like. Accordingly, the term "liquid” is to be understood in a broad sense as meaning in particular suspensions, dispersions or other mixtures or substances with liquid phases or proportions.
  • the medium 3 is preferably photoactive and / or biologically active.
  • a photosynthesis or other light 2 requiring reaction take place.
  • the medium 3 contains for this purpose the organisms to be cultivated or biologically active, preferably algae, bacteria or the like.
  • the medium 3 is highly light-scattering.
  • the medium 3 is aqueous or contains water.
  • the medium 3 may contain or at least substantially consist of fresh water, brackish water or salt water.
  • An algal suspension is particularly preferably used as medium 3.
  • the medium 3 contains an Aigenmischkultur, preferably at least in similar form in rivers, ponds o. The like.
  • Such mixed cultures are namely particularly resistant to environmental influences, diseases and / or other disorders.
  • the arrangement 1 is preferably used with algae or with an algae suspension as the medium 3, in the following description is primarily turned off on the induced by the introduced light 2 algae growth. However, these statements also apply accordingly to other organisms or photo- or bioactive media 3.
  • the arrangement 1 illustrated in FIG. 1 has an illumination device 4 and a device (supply device) 5 for introducing gas 6, which in particular contains or consists of carbon dioxide, into the medium 3.
  • the gas 6 may be, for example, flue gas, sewage gas, biogas, air or the like.
  • the gas 6 may contain a proportion of 0.04 vol.% To 100 vol.% Carbon dioxide.
  • the illumination device 4 preferably has light-conducting fibers 7, which preferably terminate in a light head 8.
  • the light 2 emerges at least substantially or exclusively at the end of the fibers 7 or at the light head 8 - in particular bundled or directed.
  • the light head 8 or the illumination device 4 is preferably arranged above a surface 9 of the medium 3.
  • preferably sunlight is collected with a light collecting device 10, in particular a collector. It is understood that lighting by artificial light with appropriate measures is possible.
  • the supply device 5 has a suitable introduction means 11, which is shown only schematically in FIG. 1 and from which the gas 6 escapes, in particular in the form of gas bubbles 12.
  • the introduction means 11 is, for example, a bubble stone or the like, through which the supplied gas is introduced into the medium 3 in a bubble-like manner, for example.
  • the gas is introduced into the medium 3 preferably via or through a bottom 13 of a container 14, which serves to receive the medium 3.
  • the light head 8 is arranged above the medium 3, preferably opposite the feed device 5.
  • the light head 8 and the feed device 5 are arranged at least substantially coaxially to a rotation axis X of the medium 3, as will be discussed in greater detail below.
  • the arrangement 1 has an agitator 15 for circulating or moving the medium 3, in particular for producing a circular or rotary flow S of the medium 3 or in the medium 3.
  • the agitator 15 preferably forms a device for generating a bubble chain 16 from the gas 6 introduced into the medium 3 or from gas bubbles 12 rising in the medium 3.
  • the agitator 15 preferably has a plurality of vanes or stirring elements 17 for setting the medium 3 in motion, in particular for generating a rotary, circular or spiral flow S or a vortex or rotation of the medium 3 or in the medium 3 that the bubble chain 16 is formed.
  • the term "bubble chain” is to be understood here as a gas tunnel or a region of the medium 3 which is particularly permeable or transparent to the light 2, primarily consisting of gas 6.
  • the agitator 15 is preferably arranged below in the container 14, in particular at or on the bottom 13 of the container 14.
  • the agitator 15 has in the illustrated embodiment, an axis of rotation X, about which the agitator 15 and a rotor of the agitator 15 and the back elements 17 is rotatable or are.
  • the medium 3 is offset by the rotating stirring elements 17 in a rotational, circular or spiral movement about the axis of rotation X, so that the bubbles 12 to the bubble chain 16 meet, preferably at least substantially straight or linear preferably extends upwardly from the bottom 13 of the container 14.
  • a helical or other bubble chain 16 can also be formed.
  • the container 14 preferably has a cylindrical shape with smooth inner walls, which is conducive to the preferred embodiment of a uniform rotational flow of the medium 3 about the axis of rotation X.
  • turbulent flows do not occur which can lead to tearing off of the bubble chain 16.
  • the bubble chain 16 with the illumination device 4 is illuminated.
  • the light emerging from the light head 8 light 2 is coupled into the bladder 16, wherein the bladder 16 acts as a light guide.
  • the losses of a bubble chain 16 can be determined via the radiated intensity as a function of the length, for example, green light having resulted in an attenuation of approximately 1.5 dB / m.
  • the bubbles 12 rotate, the delivery of the scattered light from the bubble chain 16 occurs radially in all directions.
  • the bubble chain 16 or a bubble vortex can be produced and utilized for light distribution, above all in optically denser suspensions, such as, for example, algae suspensions.
  • the introduction of the light 2 into the medium 3 preferably takes place via the bubble chain 16.
  • the bubble chain 16 is preferably provided substantially from the bottom region to the surface 9 of the medium 3, wherein the illumination of the bubble chain 16 is preferably provided in the exit region 18 of the gas bubbles 12 from the medium 3 or at the surface 9 and wherein the light 2 preferably in directed vertically and / or focused on the exit region 18.
  • the light head 8 is preferably spaced sufficiently far from the surface 9 of the medium 3 in order to be able to preclude wetting of the light head 8 with the medium 3. As a result, there is no reason to fear that growing algae on the light head 8 will reduce the transparency of the light head 8 and the illuminance.
  • a detection device 19 may be provided which is connected via a control device 20 to a motor 21 of the agitator 15 and / or to the light head 8.
  • the light head 8 or the light 2 can preferably be automatically aligned by the control device 20 with the detected exit region 18 of the bubble chain 16.
  • the light head 8 is correspondingly pivotable or movable, so that it is always possible to couple light 2 into the bubble chain 16, preferably via its exit region 18. As a result, sufficient illumination of the bubble chain 16 is always ensured even if the position of the exit region 18 changes due to the moving medium 3.
  • the detection device 19 preferably operates optically.
  • the detection device 19 may include or be formed by a camera.
  • the detection device 19 may alternatively or additionally to the determination or tracking of the exit region 18 also generally detect the position and / or shape of the bubble chain 16 or the course of the gas bubbles 12 in the medium 3. However, a measuring device, not shown, may additionally be provided for this purpose.
  • the detection device 19 and / or the measuring device also a determination of a flow profile and / or the flow velocity of the medium 3 take place.
  • the control device 20 can in particular control or regulate the feed device 5, the agitator 15 or the motor 21 and / or the light coupling or the light head 8.
  • the control or regulation can initially be carried out in such a way that the bubble chain 16 or a bubble chain 16 which is stable in its position is produced. Further, the control or regulation can cause the light 2 is always directed to the outlet region 18 of the bubble chain 16 or to any other desired area of the bubble chain 16 or remains, in particular as needed tracked.
  • the control device 20 can, for example, control or regulate the motor 21 or the agitator 15 as a function of the flow velocity and / or the flow profile 1 of the medium 3 and / or depending on the occurrence or the position of the outlet region 18, by the rotational speed of the flow S, the medium 3, the agitator 15 and the stirring elements 17 to control or regulate and a desired flow profile and / or a desired flow rate of the medium 3 and thus to achieve the formation of a particular shape, location and size of the bladder 16 ,
  • gas 6 is preferably introduced into the medium 3 between the rotating stirring elements 17 or along the axis of rotation X.
  • FIG. 2 is a fragmentary enlargement of FIG. 1, with some components and components being shown in greater detail and with the medium 3 omitted for simplicity.
  • the agitator 15 has a drive shaft 28, which can be driven directly or indirectly by the motor 21.
  • the drive shaft 28 carries a rotor with or formed from the stirring elements 17 in the container 14.
  • the introduction means 11 is preferably arranged on the agitator 15 or its rotor or between the agitating element 17 or above it and / or in the axis of rotation X.
  • the agitator 15 or the rotor or the drive shaft 28 is provided with a receptacle 29 for the introduction means 1 1.
  • the receptacle 29 is attached to the drive shaft 28. arranges, in the illustrated example at the end of the drive shaft 28 in the container 14.
  • the receptacle 29 is, for example, pot-shaped or cup-shaped.
  • the introduction means 1 1 is preferably in the receptacle 29, in particular in an axial recess of the receptacle 29, used, for example glued.
  • the supply of the gas 6 is preferably carried out by the drive shaft 28.
  • the drive shaft 28 is for this purpose in particular - at least partially - hollow.
  • the drive shaft 28 has an axial bore 30, for example via a particular radial opening
  • the bore 30 extends in particular from the free, lying in the container 14 end of the drive shaft 28 to the opening 31. However, other constructive solutions are possible.
  • the bore 30 may also be any other channel or the like.
  • the introduction means 1 1 is connected by means of the receptacle 29 for supplying the gas 6 to the drive shaft 28 and bore 30. This forms a gas supply path.
  • the introduction means 1 1 is thus assigned to the agitator 15 or forms part of it.
  • the feed device 5 preferably has a check valve 32.
  • the check valve 32 is preferably disposed in the gas supply path.
  • the check valve 32 is arranged on the stirrer 15 or on the drive shaft 28, in the illustrated example at the end of the drive shaft 28 or bore 30 or in the receptacle 29.
  • check valve 32 prevents the medium 3 or components of the medium 3, such as water, from entering the gas supply path or the supply device 5 or further parts thereof when the gas supply is turned off.
  • the check valve prevents the medium 3 or components of the medium 3, such as water
  • the arrangement 1 or the agitator 15 preferably has a housing 33, which is preferably at least substantially cylindrical or sleeve-like in the illustrated embodiment.
  • the housing 33 forms a feed space 34 for the supply of gas 6 to the opening 3 1. Accordingly, the housing 33 covers the opening 31 and extends along the drive shaft 28.
  • the gas supply into the space 34 and in the housing 33 takes place in the illustrated example in particular via a connecting piece, a line 35 or the like, as indicated by way of example in FIG. 2.
  • the housing 33 preferably also serves to support the drive shaft 28.
  • the drive shaft 28 may be supported via a guide ring 36 or another bearing on the drive-side or motor-side end.
  • the drive shaft 28 is mounted in the illustrated example, in particular via a (further) guide ring 37 or any other suitable bearing.
  • This is associated, for example, a bottom plate 38, which is screwed in the illustrated example by means of screws 39 to the bottom 13.
  • a (further) guide ring 37 or any other suitable bearing.
  • This is associated, for example, a bottom plate 38, which is screwed in the illustrated example by means of screws 39 to the bottom 13.
  • other constructive solutions are possible.
  • the drive shaft 28 is preferably mounted twice, in particular here via the guide rings 36 and 37 or other bearings.
  • the guide rings 36 and 37 or other bearings are preferably mounted twice, in particular here via the guide rings 36 and 37 or other bearings.
  • the housing 33 preferably extends between the two bearing points of the drive shaft 28.
  • the housing 33 is preferably connected to the bottom 13 and / or the bottom plate 38, for example, welded.
  • a seal 40 is preferably arranged between the bottom 13 and the bottom plate 38.
  • the drive shaft 28 is preferably sealed in the region of the bottom 13 or the bottom plate 38 on the one hand and / or on the other hand in the region of the drive-side or motor-side end.
  • seals 41 and 42 in particular Simmerringe or the like, are provided. However, other seals may be provided.
  • the seals 41 and 42 preferably seal between the drive shaft 28 on the one hand and the housing 33 on the other hand.
  • other constructive solutions are possible.
  • the seals 41 and 42 or Simmerringe or the like are preferably covered by associated flanges, sleeves 43 and 44 or the like and / or axially secured or held.
  • the sleeves 43 and 44 are preferably made of copper or brass or other suitable material.
  • the drive shaft 28 is connected or coupled to the motor 21 preferably via a clutch 45 and / or an unillustrated transmission or the like.
  • the coupling 45 may be a rigid connection or another connection, in particular a slip clutch or the like.
  • the present invention proposes a multifunctional bottom for photobioreactors.
  • the proposed arrangement 1 and the proposed method allow the supply of gas 6 through the agitator 15 or its drive shaft 28 and along the axis of rotation X of the agitator 15 in the container 14 and in the medium 3.
  • the gas 6 in particular via the line 35 passed into the supply chamber 34 of the housing 33 and passed from there via the opening 3 1 and the bore 30 through the check valve 32. Subsequently, the gas 6 flows through the receptacle 29 and the introduction means 11 into the container 14 or the medium 3.
  • a bubble chain 16 is preferably formed from the gas bubbles 12 during rotation of the agitator 15, as already explained.
  • the arrangement 1 or the housing 33 may have a drain, not shown, a liquid separator, an associated pump or the like, in order to be able to discharge any leaking liquid or escaping medium 3 from the housing 33 in the event of a leak.
  • the supply device 5 or the introduction means 1 1 is preferably formed by the agitator 15 or integrated in this.
  • the introduction of the gas 6 is at least substantially along the axis of rotation X of the agitator 15.
  • the container 14 preferably has at least substantially a cylindrical shape.
  • the container 14 can also have any other suitable shape, in particular if a plurality of flows S and / or a plurality of bubble strands or bubble chains 16 are produced in the container 14.
  • the arrangement 1 or the container 14 can also contain or have a plurality of agitators 15 and / or feed devices 5 for introducing gas 6.
  • the illumination of the bubble vortex or of the bubble chain 16 can preferably take place from above, alternatively or additionally, but also from the side and / or from below.
  • the illumination device 4 or at least one light pipe or fiber 7 for supplying light and / or the light head 8 can also be integrated into the multifunctional floor 13, the agitator 15 and / or the feed device 5 or the introduction device 11.
  • FIG. 3 shows a portion of the bottom 13 with the agitator 15 including its drive and the feeder 5.
  • the introduction of the supplied gas 6 takes place in the hollow drive shaft 28 preferably axially and / or only along or within the or by the drive shaft 28.
  • the motor 21 is preferably arranged here transversely or radially to the drive shaft 28 or rotational axis X.

Abstract

Pour la culture d'organismes, du gaz est introduit dans un récipient contenant un fluide renfermant les organismes, à travers un mélangeur ou à travers l'arbre d'entraînement du mélangeur, ou le long d'un axe de rotation du mélangeur.
PCT/EP2010/007000 2009-11-20 2010-11-17 Dispositif et procédé de culture d'organismes WO2011060926A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE112010004498T DE112010004498A5 (de) 2009-11-20 2010-11-17 Anordnung und Verfahren zur Kultivierung von Organismen

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
EP09014525.1 2009-11-20
EP09014525 2009-11-20
EP09014875.0 2009-12-01
EP09014875 2009-12-01
EP10000900.0 2010-01-29
EP20100000900 EP2325293B1 (fr) 2009-11-20 2010-01-29 Appareil et procédé de répartition tridimensionnelle de rayonnement électromagnétique dans un milieu liquide
EP10005851 2010-06-07
EP10005851.0 2010-06-07

Publications (1)

Publication Number Publication Date
WO2011060926A1 true WO2011060926A1 (fr) 2011-05-26

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DE (1) DE112010004498A5 (fr)
WO (1) WO2011060926A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE460612C (de) * 1924-05-20 1928-06-04 Hans Scheidemandel Dr Verfahren zum Durchgasen von Fluessigkeiten
KR20040025203A (ko) * 2002-09-18 2004-03-24 학교법인 인하학원 기포탑 광생물반응기 및 이를 이용한 광합성 미생물의배양방법
US20050239182A1 (en) * 2002-05-13 2005-10-27 Isaac Berzin Synthetic and biologically-derived products produced using biomass produced by photobioreactors configured for mitigation of pollutants in flue gases
WO2007050971A1 (fr) * 2005-10-26 2007-05-03 Levtech, Inc. Bioreacteur equipe d'un melangeur et d'un aerateur
DE102007021244A1 (de) * 2007-05-07 2008-01-03 Karl-Heinz Zacher Gaseintragsrotor zur Gaseintragung in Bioreaktoren für die Kultivierung von Mikroorganismen
WO2008135276A2 (fr) 2007-05-07 2008-11-13 Universität Duisburg-Essen Ensemble et procédé de répartition tridimensionnelle de la lumière dans un milieu liquide

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE460612C (de) * 1924-05-20 1928-06-04 Hans Scheidemandel Dr Verfahren zum Durchgasen von Fluessigkeiten
US20050239182A1 (en) * 2002-05-13 2005-10-27 Isaac Berzin Synthetic and biologically-derived products produced using biomass produced by photobioreactors configured for mitigation of pollutants in flue gases
KR20040025203A (ko) * 2002-09-18 2004-03-24 학교법인 인하학원 기포탑 광생물반응기 및 이를 이용한 광합성 미생물의배양방법
WO2007050971A1 (fr) * 2005-10-26 2007-05-03 Levtech, Inc. Bioreacteur equipe d'un melangeur et d'un aerateur
DE102007021244A1 (de) * 2007-05-07 2008-01-03 Karl-Heinz Zacher Gaseintragsrotor zur Gaseintragung in Bioreaktoren für die Kultivierung von Mikroorganismen
WO2008135276A2 (fr) 2007-05-07 2008-11-13 Universität Duisburg-Essen Ensemble et procédé de répartition tridimensionnelle de la lumière dans un milieu liquide

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BARBOSA M J ET AL: "Overcoming Shear Stress of Microalgae Cultures in Sparged Photobioreactors", BIOTECHNOLOGY AND BIOENGINEERING 20040105 US LNKD- DOI:10.1002/BIT.10862, vol. 85, no. 1, 5 January 2004 (2004-01-05), pages 78 - 85, XP002619623, ISSN: 0006-3592 *
BERBEROGLU, H. ET AL.,: "Light-transfer in bubble sparged photobioreactors for H2 production and CO2 mitigation", INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol. 32, no. 13, 2007, pages 2273 - 2285, XP002619624 *
BUWA, V.V. AND RANADE, V.V.: "Dynamics of gas-liquid flow in a rectangular bubble column: experiments and single/multi-group CDF stimulations", CHEMICAL ENGINEERING SCIENCE, vol. 57, no. 22-23, 2007, pages 4715 - 4736, XP002619622 *
DATABASE WPI Week 200446, Derwent World Patents Index; AN 2004-485505, XP002592692 *
OH S K W ET AL: "Further studies of the culture of mouse hybridomas in an agitated bioreactor with and without continuous sparging", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 22, no. 3, 1 February 1992 (1992-02-01), pages 245 - 270, XP023705249, ISSN: 0168-1656, [retrieved on 19920201], DOI: DOI:10.1016/0168-1656(92)90144-X *
TAKAHASHI K ET AL: "BUBBLE SIZE DISTRIBUTIONS IN IMPELLER REGION IN A GAS SPARGED VESSEL AGITATED BY A RUSHTON TURBINE", JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, SOCIETY OF CHEMICAL ENGINEERS, JP, vol. 25, no. 4, 1 August 1992 (1992-08-01), pages 427 - 432, XP000332078, ISSN: 0021-9592, DOI: DOI:10.1252/JCEJ.25.73 *
UGWU ET AL: "Photobioreactors for mass cultivation of algae", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB, vol. 99, no. 10, 13 March 2008 (2008-03-13), pages 4021 - 4028, XP022526206, ISSN: 0960-8524, DOI: DOI:10.1016/J.BIORTECH.2007.01.046 *

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