WO2011052462A1 - 夜間照明による植物への光害の評価方法、夜間照明の特性評価方法及び光害回避照明装置 - Google Patents
夜間照明による植物への光害の評価方法、夜間照明の特性評価方法及び光害回避照明装置 Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- H—ELECTRICITY
- H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
- H05B—ELECTRIC HEATING; ELECTRIC LIGHT SOURCES NOT OTHERWISE PROVIDED FOR; CIRCUIT ARRANGEMENTS FOR ELECTRIC LIGHT SOURCES, IN GENERAL
- H05B47/00—Circuit arrangements for operating light sources in general, i.e. where the type of light source is not relevant
- H05B47/10—Controlling the light source
- H05B47/16—Controlling the light source by timing means
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02B—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO BUILDINGS, e.g. HOUSING, HOUSE APPLIANCES OR RELATED END-USER APPLICATIONS
- Y02B20/00—Energy efficient lighting technologies, e.g. halogen lamps or gas discharge lamps
- Y02B20/40—Control techniques providing energy savings, e.g. smart controller or presence detection
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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- the present invention relates to a method for evaluating light damage to plants by night lighting, a method for evaluating characteristics of night lighting, and a light pollution avoiding lighting apparatus.
- a white or yellow light source such as a mercury lamp, a fluorescent lamp, or a sodium lamp is generally used.
- a night illumination device using a light source is installed on a road or the like close to a vegetation area such as a field, the light is also irradiated to plants in the vegetation area at night. Decreased yield and reduced quality due to delayed flowering of short-day plants such as rice and soybeans, or early flowering of long-day plants such as spinach and broccoli. This is called light pollution.
- Patent Document 1 a reflecting mirror and a light source are arranged inside a lamp body having an open bottom surface, and a light shielding wall is formed integrally with the lamp body below the light source behind the light center of the light source.
- a road illuminator in which a part of the irradiation light is shielded is described.
- Patent Document 2 discloses a green filter with a light quality balance in which a slurry mixed with zinc oxide and a green inorganic pigment is deposited on the outer surface of a fluorescent mercury lamp to cut ultraviolet radiation, reduce blue light, and increase green light. It describes about reducing the growth radiation efficiency of a plant with an attached lamp.
- Patent Document 3 has a spectral radiant energy peak between the lower limit of the wavelength range where the influence on flower bud formation on plants is strong and the lower limit of the wavelength range where the antifungal effect is high, and the spectral radiant energy ⁇ e ( ⁇ ). And a luminaire having a light source in which the ratio of the product of spectral absorption Pr ( ⁇ ) of phytochrome in the section of 560 to 700 nm and the radiant flux included in the wavelength range of 460 to 580 nm is 0.05 or less Is described.
- Patent Document 4 uses light from a light source that emits green light of 500 to 580 nm to irradiate a range including the vegetation area side as a street lamp for installation at a position close to the vegetation area.
- a street lamp is described that uses a combination of LEDs with high directivity in a wavelength range other than green of 500 to 580 nm for irradiation to a side that is not a vegetation region.
- Patent Document 5 discloses a near-infrared fluorescent lamp that irradiates near-infrared light and shields red light by applying a pigment containing a near-infrared light-emitting phosphor, a blue-light-emitting phosphor, and a green light-emitting phosphor as main components as an inner surface paint.
- Patent Document 6 describes that near-infrared light is irradiated to paddy rice before sunlight is irradiated to prevent the heading delay of paddy rice.
- Non-Patent Document 1 discloses a dark period interruption in which flowering delay of short-day plants is caused by light irradiation for only 10 minutes of nighttime over several hours, regarding the relationship between flowering time control and nighttime light irradiation.
- Non-Patent Document 2 as the relationship between photosynthesis and pulse emission, which are important functions of plants, the photosynthesis rate of Sardana when the pulse emission frequency is 2.5 kHz and the duty ratio is 50% is that of continuous light. It is described with respect to an increase of 23% compared to the case.
- Patent Document 1 light damage to the plant is avoided by shielding a part of the illumination light source, but there is an abrupt change in illuminance between the illuminated bright area and the darkness due to the shading adjacent to the illuminated area. This creates the potential for danger in the darkness, which is rather unsafe.
- the illumination light source used for avoiding light pollution is illumination with green-centered light, making it difficult to illuminate natural colors with high color rendering properties and limited applications. Is done.
- JP 2000-222911 A JP 2005-216572 A JP 2004-121217 A JP 2008-226567 A JP 2006-244910 A JP 2006-271374 A
- the present invention has been made to solve the above-mentioned problems, and the method for evaluating light damage to plants by night illumination according to the present invention is based on light damage caused to a plant by a lighting device installed in the vicinity of a vegetation area.
- a multi-spot plant in the meteorological instrument until the expression of the flowering induction gene. After planting and planting some plants in an artificial meteor for performing night illumination after expression of the flowering-inducing gene, and after having passed one or more night periods, the leaf blades are collected and processed.
- Total RNA is extracted, and the expression level of the target gene is quantitatively analyzed to determine the gene expression level A under night-lighting conditions, and after expression of the flowering induction gene, Artificial air without night lighting After putting a plant of another strain in the vessel and letting go through one or more night periods, extract the leaf blades and extract the total RNA, and determine the mRNA level of the gene for the target gene expression level. Quantitative analysis is performed to determine the gene expression level B under night illumination conditions, and the gene expression level A under night illumination conditions and gene expression level B under night illumination conditions. And determining the light pollution avoidance ratio as a ratio. When the plant is rice, the flowering induction gene is Hd3a.
- the present invention further includes obtaining an average color rendering index for the light source used for the night illumination, and the light pollution avoidance ratio and the average color rendering evaluation obtained by the method for evaluating light damage to plants by the night illumination. It is good also as the characteristic evaluation method of the night illumination which evaluated the characteristic of the light source of night illumination from the number.
- the flowering induction gene is Hd3a.
- the light pollution avoiding illumination device has a specific color component pulsed light source or a different color component driven by a pulse driving device at a predetermined pulse emission frequency and duty ratio.
- An illumination device using a light source combining a plurality of types of pulsed light sources that are driven at a pulsed light emission frequency and duty ratio, wherein the pulsed light source of the specific color component or a plurality of types of pulsed light sources are combined.
- the pulse light source of the specific color component or a plurality of types of pulse light sources are combined so that the light damage avoidance ratio obtained by the method for evaluating light damage to plants by night illumination with respect to the light source is 0.8 or more Set the pulse emission frequency and duty ratio of each type of pulsed light source A.
- the blue light emitting diode is driven at a pulse light emission frequency of 300 to 2700 Hz and a duty ratio of 10 to 90%.
- the green light emitting diode is a green light emitting diode
- the green light emitting diode is driven at a pulse light emission frequency of 50 to 1000 Hz and a duty ratio of 20 to 90%
- the pulse light source of the specific color component is a yellow green light emitting diode.
- the yellow-green light emitting diode is preferably driven at a pulse emission frequency of 500 to 2700 Hz and a duty ratio of 10 to 90%.
- the light pollution avoiding illumination apparatus is an illumination apparatus using a light source that is a combination of pulsed light sources each having a different color component and driven by a pulse driving device at a predetermined pulsed light emission frequency and duty ratio.
- the light damage avoidance ratio determined by the method for evaluating light damage to plants by night illumination with respect to a light source combining the plurality of kinds of pulsed light source is 0.8 or more.
- the plurality of types of pulsed light sources that set the pulsed light emission frequency and duty ratio of each type of pulsed light source in the light source that combines the above-mentioned pulsed light sources, and that the average color rendering index as the whole light source is 60 or more It may be a combination of the above.
- the plurality of pulsed light sources may include at least a blue light emitting diode and a yellow-green light emitting diode, and the plurality of pulsed light sources may include a blue light emitting diode, a green light emitting diode, and a yellow green light emitting diode. .
- the present invention it is possible to appropriately and effectively evaluate light damage caused by night lighting by using the light damage avoidance ratio from the gene expression level obtained under night lighting conditions and without night lighting conditions.
- equipment for evaluating light pollution it is possible to use simple equipment and measuring instruments by using an artificial meteorograph, and the expression level of a flowering induction gene that starts expression before actual flowering can be determined. Since it is used and evaluated, it can be carried out in a form in which the time is shorter than the conventional method of observing flowering. In the case of rice, when observing outdoors, the evaluation of light pollution is usually carried out once a year, but in the case of the present invention using an artificial meteor, the evaluation of light pollution is 5 weeks after emergence.
- the average color rendering index of the light source for night illumination can be obtained, and the suitability as a night illumination device can be evaluated together with the light pollution avoidance ratio.
- the illumination apparatus using the pulsed light source controlled to the optimum pulsed light emission frequency and duty ratio derived by the nighttime illumination characteristic evaluation method of the present invention is included in the phytochrome absorption wavelength range of 560 to 700 nm.
- the out-of-light avoidance ratio can be increased to 1 or more for blue and green that are outside this wavelength range and originally considered to be less likely to cause light pollution.
- appropriately controlled pulsed blue, green, and yellow-green illuminations provide higher illumination while maintaining equivalent color rendering compared to other general lighting (such as mercury lamps). Even if you go, you can make little light pollution to the crops (rice).
- the illuminating device is constituted by a light source in which a plurality of types of pulsed light sources having different color components are combined, and light is obtained by appropriately setting the pulse emission frequency and duty ratio of each type of pulsed light source.
- It can be a harm avoidance lighting device.
- a pulsed light source it is possible to select a light source element that has a higher light damage avoidance ratio than a continuous light source, and by combining various light emission frequencies and duty ratios, the selectivity of the light source element become diverse.
- it can be a combination of a plurality of types of pulsed light sources that give a natural sense to humans.
- (A)-(f) It is a figure which shows the evaluation of the light pollution avoidance about an illumination light source, and the evaluation of the characteristic as night illumination as a flow.
- the present invention when evaluating the light pollution by night illumination, data is obtained about a sample collected from a plant cultivated in an artificial meteor that performs night illumination, and flowering-induced gene expression is analyzed to analyze night illumination.
- light damage to plants was evaluated by determining the expression level of flowering-inducible genes when pulse light emission type light source was used as the light source and the frequency and duty ratio of pulse light emission were varied. We are studying the conditions for the avoidance lighting device.
- RNA extraction 50-65 mg of freshly stored leaf blades were weighed into a 2 mL sample tube, and crushed using a pulverizer (Automill: Tokken Co., Ltd.) while frozen in liquid nitrogen.
- a pulverizer Automill: Tokken Co., Ltd.
- RNeasy Plant Mini Kit Qiagen Co., Ltd.
- total RNA was extracted by an automatic extraction apparatus (QIAcube: Qiagen Co., Ltd.) according to the reagent and protocol of the apparatus.
- the purity and concentration of the extracted RNA were measured with an absorptiometer (Nanodrop ND-1000: Thermo Fisher Scientific).
- the expression level of Hd3a mRNA was shown by a relative quantification method using ubiquitin (ubq) mRNA as an internal standard gene.
- the primer set for quantifying the expression level of ubiquitin mRNA was as shown below.
- Reverse primer 5'-ACGATTGATTTAACCAGTCCATGA-3 '(SEQ ID NO: 4) Based on the above, the ratio of the Hd3a-mRNA expression level to the ubq-mRNA expression level was taken as the relative expression level of Hd3a-mRNA.
- A is the gene expression level under night illumination
- B is the gene expression level under no night lighting
- a / B Use light pollution avoidance ratio. The higher the light pollution avoidance ratio, the lower the possibility of light pollution. Calculate the light pollution avoidance ratio for each combination of the wavelength, pulse emission frequency, and duty ratio of the light source of the night illumination used in the artificial meteor that obtained the rice sample in the process of (1) to (4) above. . Through such a process, the light pollution avoidance of the illumination light source is evaluated.
- an average color rendering index Ra is obtained by a spectroscopic method for the color of the light source for night illumination.
- Table 1 generally shows the numerical range of the color rendering property Ra that is adapted to the usage application of illumination.
- the right column shows the corresponding lamp type.
- Fig. 1 shows the flow.
- the period until the expression of the flowering induction gene is confirmed is Although it is about 5 weeks, it can be said that this period is almost constant for the same type of plant, so after confirming the expression of this flowering induction gene once, the expression of the flowering induction gene is again performed for the same type of plant.
- the process may move to the next stage in the flow of FIG.
- “until the expression of a flowering induction gene is confirmed” has a meaning including the fact that it is considered to be expressed on such a probability.
- real-time PCR is used in the flow of FIG. 1.
- a method for quantitatively analyzing mRNA for example, Northern blotting is used. Other analysis methods may be used.
- FIG. 2 shows the light pollution avoidance ratio obtained according to the frequency.
- (b) blue, (c) green, and (d) yellow-green have relatively high light pollution avoidance ratios, and (e) yellow has a lower value than those, and the light pollution avoidance ratios to some extent depend on the pulse emission frequency. I can see that they are different. In the case of (a) purple and (f) red, the light pollution avoidance ratio is very low.
- FIG. 4 shows the case of yellow-green LED as shown in FIG.
- the blue light in FIG. 3 has a particularly high light damage avoidance ratio at a pulse emission frequency of 700 Hz and a duty ratio of 60%
- the green light in FIG. 4 has a particularly high light damage avoidance ratio at a pulse light emission frequency of around 50 Hz, a duty ratio of 60% and 90%.
- the light pollution avoidance ratio is particularly high near the pulse emission frequency of 700 Hz and the duty ratio of 70%.
- the light pollution avoidance ratio is an amount defined in the form of A / B from the gene expression level under night illumination (A) and the gene expression level under night illumination conditions (B). Although it is used as an index for representing the sample as a ratio corresponding to the ratio of avoiding light pollution, the quantity is not expressed as the ratio to the whole. Therefore, as a result shown in FIGS. 2 to 5, the high numerical value of the light pollution avoidance ratio exceeds 1.0. It represents a quantity that is an index as a relative ratio rather than an absolute ratio.
- the value of the light pollution avoidance ratio of the illumination light source is preferably a value of 1.0 or more, but in practice, if the light pollution avoidance ratio is 0.8 or more, it is considered to be practically used. In the case of green light, the light pollution avoidance ratio is about 0.70 for continuous light, and 1.34 for pulse emission with 50 Hz and a duty ratio of 60%. From the results shown in FIGS. As for light and yellow-green light, those suitable as the light pollution avoidance ratio can be obtained by appropriately selecting the pulse emission frequency and the duty ratio.
- the blue light emitting diode is driven at a pulse emission frequency of 300 to 2700 Hz and a duty ratio of 10 to 90%, and from FIG.
- a diode it is assumed to be driven with a pulse emission frequency of 50 to 1000 Hz and a duty ratio of 20 to 90%.
- the pulse emission frequency is 500 to 2700 Hz and the duty ratio is 10 to 90%. It should be driven.
- a pulse light emission type light source is used as the light source, but the light damage avoidance ratio for each color light source at the time of continuous light emission at an illuminance of 5 lux is shown in FIG. Show.
- the numerical values in the figure indicate the light pollution avoidance ratio, and the error bars indicate the standard deviation.
- the light pollution avoidance ratio is 0.8 or more only in blue, particularly yellow-green, yellow, which is within the absorption wavelength range of 560 nm to 700 nm of phytochrome having strong influence on flowering described in Patent Document 3. It can be seen that the red light avoidance ratio is low under continuous light conditions. Further, when FIG. 6 is compared with the cases of FIGS. 3 to 5, it can be seen that the effect of improving the light damage avoidance ratio of blue, green and yellow-green by the pulse emission frequency and the duty ratio becomes more remarkable.
- the average color rendering index Ra is not so high.
- an illuminating device in which blue, green, and yellow-green LEDs were combined was configured, and measurements were performed under the same conditions.
- FIG. 7 shows a light emission spectrum by an example of a light pollution avoiding lighting device configured by combining a plurality of blue, green, and yellow-green LEDs.
- the white light spectrum is a dotted line, and the green LED spectrum is a chain line. It is shown by.
- This light pollution avoidance lighting device has a blue LED emission frequency of 250 Hz, a green LED emission frequency of 250 Hz, a yellow-green LED emission frequency of 1000 Hz, and a duty ratio of 10%.
- the emission spectrum of an example of a light pollution avoiding LED illumination light source configured by combining a plurality of blue, green and yellow-green LEDs is shown by a solid line, and the emission spectrum of a mercury lamp illumination light source generally used for night illumination. Is indicated by a dotted line, and the emission spectrum of the fluorescent lamp illumination light source is indicated by a chain line.
- This light pollution avoiding LED illumination light source uses a combination of a pulse emission frequency and a duty ratio in which the light source of each color in FIG. 3 to FIG. 5 shows the highest light pollution avoidance ratio.
- the frequency is 700 Hz
- the duty ratio is 60%
- the emission frequency of the green LED is 50 Hz
- the duty ratio is 60%
- the emission frequency of the yellow-green LED is 700 Hz
- the duty ratio is 70%.
- FIG. 9 shows the results of a test conducted in the sense of confirming the light pollution avoidance effect of the light pollution avoidance lighting apparatus described above.
- Each of the three artificial weather devices was installed with the light pollution avoidance lighting apparatus. Things (mixed color: pulsed light emission), those with the same spectral spectrum as the light pollution avoiding lighting device but with continuous lighting (mixed color: continuous light emission), those without a lighting device (target zone: night lighting) None), rice (12 strains) was cultivated in each, and the number of flowering arrival days was observed.
- the rice with the light pollution avoiding lighting device shows a two-day delay at the start of flowering compared to the rice without night lighting, but with a flowering rate of 50%, the one-day delay is shown. And no delay at the end of flowering.
- the start of flowering was 5 days, 50% flowering showed a delay of 4 days, and one strain did not flower even 88 days after emergence.
- the number of days that the flowering rate reaches 50% is regarded as an index of rice flowering among producers and researchers.
- Fig. 10 shows the flowering delay days for light pollution avoidance LED lighting, fluorescent lighting, and mercury lamp lighting installed in an outdoor paddy field where rice (Koshihikari) is actually cultivated.
- This is an average horizontal plane illuminance of 3 lux, which is an illuminance standard class B of a security light by the Japan Security Equipment Association, an average horizontal plane illuminance of 5 lux, which is also a class A, International Lighting Commission Pub. 115 indicates the flowering delay days with an average horizontal illuminance of 10 lux on the “roads with high night use” in “Lighting conditions for pedestrian area”, and the delay days with 3 lux are dark gray and 5 lux.
- the delay days are white, the delay days at 10 lux are shown in light gray, and the error bars indicate the standard deviation. In the LED lighting for avoiding light pollution, the delay days are only about 2 days even at 10 lux. On the other hand, fluorescent lamp illumination is delayed for 5 days or more even at 3 lux, and is delayed about 10 days at 10 lux. Mercury lamp lighting is delayed for 4 days at 3 lux, 10 days at 5 lux, 12 days at 10 lux.
- a light source by a combination of a blue LED, a green LED, and a yellow-green LED whose emission spectrum is shown in FIG. 7 is an example, and other light source elements are used as the light pollution avoiding illumination device. They can be combined.
- a light source element of the light pollution avoiding illumination apparatus a light source such as a pulse emission type LED having wavelength selectivity is used.
- a light source element may be selected on the condition that the light pollution avoiding ratio is 0.8 or more, and the blue LED, the green LED, and the yellow green whose light pollution avoiding ratio is shown in FIGS.
- the LED a plurality of monochromatic LEDs having a pulse emission frequency and a duty ratio in which the light pollution avoidance ratio is 0.8 or more may be arranged, but the lighting device can give a natural feeling to a person. Therefore, it is desirable to use an illumination device that combines LEDs of different color lights so that the average color rendering index is 60 or more for night illumination.
- the emission frequency of the blue LED is 250 Hz
- the emission frequency of the green LED is 250 Hz
- the emission frequency of the yellow-green LED is 1000 Hz
- the duty ratio is 10% for both.
- the LEDs of the respective colors may have other emission frequencies and have different duty ratios, and may have other emission frequencies and duty ratios. May be combined.
- the illumination device includes an element light source such as an LED and a pulse light emission drive device that drives the element light source, and the pulse light emission drive device is provided according to the light emission frequency and the duty ratio. Therefore, in terms of the simplicity of the apparatus, it is advantageous to reduce the number of emission frequencies and duty ratios as much as possible, and preferably one. Moreover, about LED, when it makes pulse light emission, the lifetime of an element can be lengthened compared with the case of continuous light emission. However, since the light emission frequency is interpersonal illumination, it should be about 30 Hz or higher, which is a critical fusion frequency that is the lower limit where a person does not feel flickering of illumination.
- the light pollution avoidance lighting apparatus combining light source elements by pulsed light emission according to the present invention avoids light pollution by setting the light pollution avoidance ratio to 0.8 or more, preferably 1.0 or more, and has an average color rendering index Ra. By setting the value to 60 or more, a natural feel can be obtained for a person.
- a natural feel can be obtained for a person.
- the light pollution avoiding lighting device according to the present invention there will be a decrease in yield as well as a decrease in price due to the lowering of the grade, but this is eliminated by using the light pollution avoiding lighting device according to the present invention, and the light of the producer It can be said that it has sufficient performance to dispel concerns about harm.
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Abstract
Description
このような状況から、夜間照明による植物への光害の評価を適切に行う方法を示すこと、また、光害を回避できる実際的かつ有効性のある照明装置を与えることが課題とされていた。
前記植物がイネの場合、開花誘導遺伝子はHd3aである。
人工気象器内でイネの開花を促進する短日条件(昼間8時間/夜間16時間)で開花誘導遺伝子の発現が確認される状態(出芽後約5週間)まで育成した。その際、人工気象器内には、波長、パルス発光周波数、デューティ比を任意の値に制御可能なLEDあるいは有機ELによる夜間照明装置を設置し、照明時の照度設定は、社団法人日本防犯設備協会による防犯灯の照度基準(SES E1901-1 クラスA)の平均水平面照度5ルクス以上とした。
冷凍保存しておいた葉身を2mLサンプルチューブに50~65mg秤量し、液体窒素で凍結した状態で粉砕機(オートミル:株式会社トッケン)を用いて粉砕した。RNA抽出にはRNイージープラント・ミニキット(RNeasy Plant Mini Kit:株式会社キアゲン)を用い、自動抽出装置(QIAcube:株式会社キアゲン)により試薬および装置のプロトコルにしたがって全RNAの抽出を行った。抽出したRNAは吸光度計(Nanodrop ND-1000:サーモ・フィッシャー・サイアンティフィク社)により純度および濃度を測定した。
(2)より得られたRNAからクァンティテクト・リバース・トランスクリプション・キット(Quantitect Reverse Transcription Kit:株式会社キアゲン)を用い、試薬のプロトコルに従いゲノム由来のDNAの除去とcDNAの合成を行った。合成したcDNAは上記の吸光度計により純度および濃度を測定した。
(3)により得られたcDNAおよびローター・ジーンSYBRグリーン・キット(Rotor-Gene SYBR Green Kit:株式会社キアゲン)と以下のプライマーセットを用いて3ローター・ジーンQ(Rotor-Gene Q:株式会社キアゲン)によりリアルタイムPCRを行い、イネ開花誘導遺伝子(Hd3a)のmRNA(メッセンジャーRNA)の発現量を定量した。
フォワードプライマー 5’-GCTCACTATCATCATCCAGCATG-3’(配列番号1)
リバースプライマー 5’-CCTTGCTCAGCTATTTAATTGCATAA-3’(配列番号2)
フォワードプライマー 5’-AACCAGCTGAGGCCCAAGA-3’(配列番号3)
リバースプライマー 5’-ACGATTGATTTAACCAGTCCATGA-3’(配列番号4)
以上から、ubq-mRNA発現量に対するHd3a-mRNA発現量の比をHd3a-mRNAの相対発現量とした。
このような過程により、照明光源についての光害回避性についての評価がなされる。
Ri=100-4.6・ΔE (i=1~15)
により求める。平均演色評価数Raは
Ra=(R1+R2+・・・・+R8)/8
により求められるものである。
Claims (11)
- 植生地域に近接して設置される照明装置が植物に及ぼす光害を評価する方法であって、
夜間照明を行うための人工気象器と夜間照明を行わない人工気象器とを用意することと、
開花誘導遺伝子の発現時まで人工気象器内で複数株の植物を育成することと、
開花誘導遺伝子の発現後に前記夜間照明を行うための人工気象器内に一部の株の植物を入れて1回以上の夜間期間を経過させた後に葉身を採取し処理して全RNAを抽出し、目的とする遺伝子の発現量について該遺伝子のmRNA量を定量的に解析することにより夜間照明条件下での遺伝子発現量Aを求めることと、
開花誘導遺伝子の発現後に前記夜間照明を行わない人工気象器内に他の株の植物を入れて1回以上の夜間期間を経過させた後に葉身を採取し処理して全RNAを抽出し、目的とする遺伝子の発現量について該遺伝子のmRNA量を定量的に解析することにより夜間照明なしの条件下での遺伝子発現量Bを求めることと、
前記夜間照明条件下での遺伝子発現量Aと夜間照明なしの条件下での遺伝子発現量Bとの比としての光害回避割合を求めることと、
からなることを特徴とする夜間照明による植物への光害の評価方法。 - 前記植物がイネであり、開花誘導遺伝子がHd3aであることを特徴とする請求項1に記載の夜間照明による植物への光害の評価方法。
- 前記夜間照明に用いられる光源についての平均演色評価数を求めることをさらに含み、
請求項1に記載の夜間照明による植物への光害の評価方法により求められた前記光害回避割合と前記平均演色評価数とから夜間照明の光源の特性を評価することを特徴とする夜間照明の特性評価方法。 - 前記植物がイネであり、開花誘導遺伝子がHd3aであることを特徴とする請求項3に記載の夜間照明の特性評価方法。
- パルス駆動装置により所定のパルス発光周波数およびデューティ比で駆動されるようにした特定色成分のパルス発光光源またはそれぞれ異なる色成分を有しパルス駆動装置により所定のパルス発光周波数およびデューティ比で駆動されるようにした複数種類のパルス発光光源を組み合わせた光源を用いた照明装置であって、前記複数種類のパルス発光光源を組み合わせた光源に対して請求項1に記載の夜間照明による植物への光害の評価方法により求められた光害回避割合が0.8以上になるように前記特定色成分のパルス発光光源または複数種類のパルス発光光源を組み合わせた光源における各々の種類のパルス発光光源のパルス発光周波数及びデューティ比を設定してなることを特徴とする光害回避照明装置。
- 前記特定色成分のパルス発光光源が青色発光ダイオードであり、該青色発光ダイオードをパルス発光周波数300~2700Hz、デューティ比10~90%で駆動するものであることを特徴とする請求項5に記載の光害回避照明装置。
- 前記特定色成分のパルス発光光源が緑色発光ダイオードであり、該緑色発光ダイオードをパルス発光周波数50~1000Hz、デューティ比20~90%で駆動するものであることを特徴とする請求項5に記載の光害回避照明装置。
- 前記特定色成分のパルス発光光源が黄緑色発光ダイオードであり、該黄緑色発光ダイオードをパルス発光周波数500~2700Hz、デューティ比10~90%で駆動するものであることを特徴とする請求項5に記載の光害回避照明装置。
- それぞれ異なる色成分を有しパルス駆動装置により所定のパルス発光周波数およびデューティ比で駆動されるようにした複数種類のパルス発光光源を組み合わせた光源を用いた照明装置であって、前記複数種類のパルス発光光源を組み合わせた光源に対して請求項1に記載の夜間照明による植物への光害の評価方法により求められた光害回避割合が0.8以上になるように前記複数種類のパルス発光光源を組み合わせた光源における各々の種類のパルス発光光源のパルス発光周波数及びデューティ比を設定するとともに、光源全体としての平均演色評価数が60以上となるような前記複数種類のパルス発光光源の組み合わせとしたことを特徴とする請求項5に記載の光害回避照明装置。
- 前記複数種類のパルス発光光源が青色発光ダイオードと黄緑色発光ダイオードを少なくとも含むことを特徴とする請求項9に記載の光害回避照明装置。
- 前記複数種類のパルス発光光源が青色発光ダイオード、緑色発光ダイオードおよび黄緑色発光ダイオードからなることを特徴とする請求項9に記載の光害回避照明装置。
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011181484A (ja) * | 2010-02-26 | 2011-09-15 | Atsuo Yoshida | 間欠照射による、植物栽培用の照明装置 |
CN103037584A (zh) * | 2012-12-20 | 2013-04-10 | 高炎华 | Led室内照明自动开启控制系统 |
CN103052231A (zh) * | 2012-12-21 | 2013-04-17 | 高炎华 | Led室内照明自动开启控制系统 |
CN103068111A (zh) * | 2012-12-21 | 2013-04-24 | 高炎华 | Led室内照明自动开启控制系统 |
JP2014060147A (ja) * | 2012-08-23 | 2014-04-03 | Yamaguchi Univ | 光害防止用の照明方法及び照明装置 |
JP2016157515A (ja) * | 2015-02-23 | 2016-09-01 | 株式会社アグリライト研究所 | Led点灯回路、ledランプ及びled点灯装置並びにこれらに用いる通電制御回路 |
WO2019031559A1 (ja) * | 2017-08-08 | 2019-02-14 | Agcグリーンテック株式会社 | 植物栽培方法、及び植物栽培装置 |
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CN111504617B (zh) * | 2020-06-05 | 2022-02-08 | 西北农林科技大学 | 一种植物生长光源确定方法及系统 |
CN114067223B (zh) * | 2022-01-17 | 2022-04-15 | 南京信息工程大学 | 一种基于夜光遥感数据的夜间城市过度照明监测方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008226567A (ja) * | 2007-03-12 | 2008-09-25 | Yamaguchi Univ | 街路灯 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4484409A (en) * | 1982-03-29 | 1984-11-27 | University Patents, Inc. | Prevention of freezing at moderate supercooling using synthetic polymeric ice nucleation inhibitors |
US20080043464A1 (en) * | 2006-08-17 | 2008-02-21 | Ian Ashdown | Bi-Chromatic Illumination Apparatus |
CN101002533A (zh) * | 2007-01-18 | 2007-07-25 | 同济大学 | 温室环境多因子协调控制算法 |
-
2010
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---|---|---|---|---|
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Non-Patent Citations (3)
Title |
---|
HARUHIKO YAMAMOTO ET AL.: "Gairoto no Yakan Shomei ga Ine no Seiiku to Shuryo ni Oyobosu Eikyo", PROCEEDINGS OF ANNUAL CONFERENCE OF THE ILLUMINATING ENGINEERING INSTITUTE OF JAPAN, DAI 39 KAI, 2006, pages 282 - 283 * |
KIYOSHI IWATANI ET AL.: "LED Kogen o Tosai shita Kogata Jinko Kishoki no Kaihatsu", THE SOCIETY OF AGRICULTURAL METEOROLOGY OF JAPAN 2008 NENDO ZENKOKU TAIKAI KOEN YOSHI, vol. 60, 2008 * |
YUSUKE FUKUSHIMA ET AL.: "Suiginto ni yoru Shuya Shomei ga Daizu no Seiiku, Shuryo Oyobi Hinshitsu ni Oyobosu Eikyo", BULLETIN OF THE FUKUOKA AGRICULTURAL RESEARCH CENTER, vol. 22, 2003, pages 43 - 47 * |
Cited By (13)
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EP3666062A4 (en) * | 2017-08-08 | 2021-04-28 | AGC Green-Tech Co., Ltd. | PLANT CULTURE PROCESS AND PLANT CULTURE DEVICE |
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