WO2011049418A3 - 테라박터 속 유래의 신규한 진세노시드 글리코시다제 및 이의 용도 - Google Patents

테라박터 속 유래의 신규한 진세노시드 글리코시다제 및 이의 용도 Download PDF

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WO2011049418A3
WO2011049418A3 PCT/KR2010/007338 KR2010007338W WO2011049418A3 WO 2011049418 A3 WO2011049418 A3 WO 2011049418A3 KR 2010007338 W KR2010007338 W KR 2010007338W WO 2011049418 A3 WO2011049418 A3 WO 2011049418A3
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protein
saponins
acid sequence
derived
ginsenoside glycosidase
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WO2011049418A2 (ko
WO2011049418A9 (ko
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안동선
김성건
이성택
임완택
이형관
김선창
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한국생명공학연구원
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Priority to CN201080047878.0A priority Critical patent/CN102782125B/zh
Publication of WO2011049418A2 publication Critical patent/WO2011049418A2/ko
Publication of WO2011049418A9 publication Critical patent/WO2011049418A9/ko
Publication of WO2011049418A3 publication Critical patent/WO2011049418A3/ko
Priority to US13/453,893 priority patent/US8735129B2/en

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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
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    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)

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Abstract

본 발명은 테라박터 속 유래의 신규한 진세노시드 글리코시다제 단백질에 관한 것으로서, 상기 단백질은 진세노시드의 특정 위치 결합 본드를 선택적으로 가수분해하여 PPD(protopanaxadiol) 타입의 사포닌을 체내 흡수 가능한 고활성 물질로 전환하는 활성을 가지고 있다. 구체적으로, 상기 단백질을 구성하는 아미노산 서열, 상기 단백질을 코딩하는 핵산 서열, 상기 핵산 서열을 포함하는 재조합 벡터, 상기 벡터로 형질전환된 형질전환체에 관한 것이며, 상기 형질전환체를 배양하여 테라박터 속 유래의 진세노시드 글리코시다제를 제조하는 방법, 상기 단백질을 이용하여 PPD 타입의 메이저 사포닌을 마이너 사포닌 형태로 전환하는 방법 및 상기 단백질을 유효성분으로 포함하는 PPD 타입 사포닌을 가용성 사포닌으로 전환하기 위한 조성물에 대한 것이다.
PCT/KR2010/007338 2009-10-23 2010-10-25 테라박터 속 유래의 신규한 진세노시드 글리코시다제 및 이의 용도 WO2011049418A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201080047878.0A CN102782125B (zh) 2009-10-23 2010-10-25 源自地杆菌属的新型人参皂苷糖苷酶及其用途
US13/453,893 US8735129B2 (en) 2009-10-23 2012-04-23 Ginsenoside glycosidase derived from the genus Terrabacter, and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090101469A KR101200571B1 (ko) 2009-10-23 2009-10-23 테라박터 속 유래의 신규한 진세노시드 글리코시다제 및 이의 용도
KR10-2009-0101469 2009-10-23

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/453,893 Continuation-In-Part US8735129B2 (en) 2009-10-23 2012-04-23 Ginsenoside glycosidase derived from the genus Terrabacter, and use thereof

Publications (3)

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WO2011049418A2 WO2011049418A2 (ko) 2011-04-28
WO2011049418A9 WO2011049418A9 (ko) 2011-10-27
WO2011049418A3 true WO2011049418A3 (ko) 2011-12-15

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US (1) US8735129B2 (ko)
KR (1) KR101200571B1 (ko)
CN (1) CN102782125B (ko)
WO (1) WO2011049418A2 (ko)

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KR101433661B1 (ko) * 2012-05-31 2014-08-26 한국과학기술원 신규한 진세노사이드 글리코시다제를 이용한 진세노사이드 ｆ2의 제조방법
KR101433660B1 (ko) * 2012-07-06 2014-08-26 한국과학기술원 신규한 진세노사이드 글리코시다제를 이용한 진세노사이드 Ｒｇ3, Ｒｈ1 및 Ｒｇ2의 제조방법
KR101340079B1 (ko) 2012-09-03 2014-01-24 한국과학기술원 테라박터 속 유래 β―글루코시다제를 이용한 ＰＰＴ 타입 진세노사이드의 생물전환
CN103146592B (zh) * 2013-01-31 2014-06-25 河南科技学院 一种转化人参皂苷Rb1生成Rd的酵母菌及其应用
CN105255970A (zh) * 2015-10-14 2016-01-20 延边大学 一种来自乳酸菌重组的β-葡萄糖苷酶制备稀有人参皂苷compound K的方法
KR20170047009A (ko) * 2015-10-22 2017-05-04 (주)아모레퍼시픽 효소적 방법에 의하여 인삼의 사포닌으로부터 컴파운드 k 및 컴파운드 y를 선택적으로 제조하는 방법
KR101785809B1 (ko) * 2015-12-18 2017-10-18 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 진세노사이드 글리코시다제를 이용한 마이너 진세노사이드의 제조방법
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CN102782125B (zh) 2014-12-17
KR20110044669A (ko) 2011-04-29
US8735129B2 (en) 2014-05-27
KR101200571B1 (ko) 2012-11-13
CN102782125A (zh) 2012-11-14
US20120264167A1 (en) 2012-10-18
WO2011049418A2 (ko) 2011-04-28
WO2011049418A9 (ko) 2011-10-27

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