WO2011039390A3 - Mutants of dna polymerase mu - Google Patents

Mutants of dna polymerase mu Download PDF

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Publication number
WO2011039390A3
WO2011039390A3 PCT/ES2010/000402 ES2010000402W WO2011039390A3 WO 2011039390 A3 WO2011039390 A3 WO 2011039390A3 ES 2010000402 W ES2010000402 W ES 2010000402W WO 2011039390 A3 WO2011039390 A3 WO 2011039390A3
Authority
WO
WIPO (PCT)
Prior art keywords
mutants
dna polymerase
polμ
overhangs
joining
Prior art date
Application number
PCT/ES2010/000402
Other languages
Spanish (es)
French (fr)
Other versions
WO2011039390A8 (en
WO2011039390A2 (en
Inventor
Luis BLANCO DÁVILA
Raquel JUÁREZ SANTOS
Original Assignee
X-Pol Biotech, S.L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by X-Pol Biotech, S.L filed Critical X-Pol Biotech, S.L
Publication of WO2011039390A2 publication Critical patent/WO2011039390A2/en
Publication of WO2011039390A8 publication Critical patent/WO2011039390A8/en
Publication of WO2011039390A3 publication Critical patent/WO2011039390A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to mutants of DNA polymerase mu (Polμ) which have greater terminal deoxynucleotidyl transferase activity than the wild enzyme (wt) and which improve the potential thereof as a molecular tool. Said mutants can be used, inter alia, in polynucleotide labelling techniques and for joining overhangs and blunt ends.
PCT/ES2010/000402 2009-10-02 2010-10-02 Mutants of dna polymerase mu WO2011039390A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ESP200901943 2009-10-02
ES200901943A ES2364010B1 (en) 2009-10-02 2009-10-02 MUTANTS OF MU POLYMERASE DNA

Publications (3)

Publication Number Publication Date
WO2011039390A2 WO2011039390A2 (en) 2011-04-07
WO2011039390A8 WO2011039390A8 (en) 2011-06-03
WO2011039390A3 true WO2011039390A3 (en) 2011-07-21

Family

ID=43826709

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2010/000402 WO2011039390A2 (en) 2009-10-02 2010-10-02 Mutants of dna polymerase mu

Country Status (2)

Country Link
ES (1) ES2364010B1 (en)
WO (1) WO2011039390A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001064909A1 (en) * 2000-03-03 2001-09-07 Consejo Superior De Investigaciones Cientificas Dna polymerase mu and uses thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001064909A1 (en) * 2000-03-03 2001-09-07 Consejo Superior De Investigaciones Cientificas Dna polymerase mu and uses thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ANDRADE, P. ET AL.: "Limited terminal transferase in human DNA polymerase mu defines the required balance between accuracy and efficiency in NHEJ.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 106, no. 38, September 2009 (2009-09-01), pages 16203 - 16208 *
DOMINGUEZ, O. ET AL.: "DNA polymerase mu (Pol mu), homologous to TdT, could act as a DNA mutator in eukaryotic cthels.", THE EUROPEAN MOLECULAR BIOLOGY ORGANIZATION JOURNAL, vol. 19, no. 7, 2000, pages 1731 - 1742, XP002144772, DOI: doi:10.1093/emboj/19.7.1731 *
JUAREZ, R ET AL.: "A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis.", NUCLEIC ACIDS RESEARCH, vol. 34, no. 16, 2006, pages 4572 - 4582 *
MOON, A.F. ET AL.: "Structural insight into the substrate specificity of DNA Polymerase mu", NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 14, no. 1, 2007, pages 45 - 53 *
MOON, A.F. ET AL.: "The X family portrait: structural insights into biological functions of X family polymerases.", DNA REPAIR, vol. 6, no. 12, 2007, pages 1709 - 1725, XP022338965, DOI: doi:10.1016/j.dnarep.2007.05.009 *
ROMAIN, F. ET AL.: "Conferring a template-dependent polymerase activity to terminal deoxynucleotidyltransferase by mutations in the Loop1 region.", NUCLEIC ACIDS RESEARCH, vol. 37, no. 14, August 2009 (2009-08-01), pages 4642 - 4656, XP002615643, DOI: doi:10.1093/nar/gkp460 *

Also Published As

Publication number Publication date
WO2011039390A8 (en) 2011-06-03
WO2011039390A2 (en) 2011-04-07
ES2364010B1 (en) 2012-06-20
ES2364010A1 (en) 2011-08-23

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