WO2011012754A3 - Sensor electroquímico para la detección de analitos en medios líquidos - Google Patents

Sensor electroquímico para la detección de analitos en medios líquidos Download PDF

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Publication number
WO2011012754A3
WO2011012754A3 PCT/ES2010/070509 ES2010070509W WO2011012754A3 WO 2011012754 A3 WO2011012754 A3 WO 2011012754A3 ES 2010070509 W ES2010070509 W ES 2010070509W WO 2011012754 A3 WO2011012754 A3 WO 2011012754A3
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WO
WIPO (PCT)
Prior art keywords
layer
polythiophene
electrochemical sensor
deposited
working electrode
Prior art date
Application number
PCT/ES2010/070509
Other languages
English (en)
French (fr)
Other versions
WO2011012754A2 (es
Inventor
Estibaliz Ochoteco Vaquero
Elena Jubete Diez
José Adolfo POMPOSO ALONSO
Hans-Jurgen Grande Telleria
Oscar A. Loaiza
German CABAÑERO
Georges Istamboulie
Thierry Noguer
Jean Louis Marty
Original Assignee
Fundacion Cidetec
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from ES200930539A external-priority patent/ES2358657B1/es
Priority claimed from ES200931247A external-priority patent/ES2362603B1/es
Application filed by Fundacion Cidetec filed Critical Fundacion Cidetec
Priority to US13/387,193 priority Critical patent/US20120181173A1/en
Priority to EP10749670.5A priority patent/EP2492351B1/en
Publication of WO2011012754A2 publication Critical patent/WO2011012754A2/es
Publication of WO2011012754A3 publication Critical patent/WO2011012754A3/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C12Q1/46—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase involving cholinesterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nanotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

La invención define un sensor electroquímico para detección de analitos en medios líquidos que comprende 4 capas, donde la primera capa (1 ) comprende un material carbonoso depositada sobre un sustrato, constituyendo dicha capa el sistema de electrodos del sensor electroquímico, compuesto al menos por un electrodo de pseudo-referencia, un electrodo de trabajo y un contraelectrodo; y la cuarta (4) comprende politiofeno depositada únicamente sobre el extremo inferior del electrodo de trabajo seleccionada entre (d1 ), que comprende una capa que comprende un politiofeno depositada sobre el extremo inferior del electrodo de trabajo y una capa que comprende un gel polimérico no conductor depositada sobre dicha capa de politiofeno;(d2), que es una capa de gel polimérico conductor que comprende un gel polimérico no conductor y un politiofeno; y (d3) que comprende una capa que comprende un politiofeno depositada sobre el extremo inferior del electrodo de trabajo y una capa que comprende nanopartículas magnéticas funcionalizadas depositada sobre dicha capa de politiofeno.
PCT/ES2010/070509 2009-07-30 2010-07-23 Sensor electroquímico para la detección de analitos en medios líquidos WO2011012754A2 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US13/387,193 US20120181173A1 (en) 2009-07-30 2010-07-23 Electrochemical sensor for the detection of analytes in liquid media
EP10749670.5A EP2492351B1 (en) 2009-07-30 2010-07-23 Electrochemical sensor for the detection of analytes in liquid media

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
ESP200930539 2009-07-30
ES200930539A ES2358657B1 (es) 2009-07-30 2009-07-30 Sensor electroqu�?mico para la detección de analitos en medios l�?quidos.
ESP200931247 2009-12-23
ES200931247A ES2362603B1 (es) 2009-12-23 2009-12-23 Sensor electroquímico para la detección de analitos en medios líquidos.

Publications (2)

Publication Number Publication Date
WO2011012754A2 WO2011012754A2 (es) 2011-02-03
WO2011012754A3 true WO2011012754A3 (es) 2011-03-24

Family

ID=42829800

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PCT/ES2010/070509 WO2011012754A2 (es) 2009-07-30 2010-07-23 Sensor electroquímico para la detección de analitos en medios líquidos

Country Status (3)

Country Link
US (1) US20120181173A1 (es)
EP (1) EP2492351B1 (es)
WO (1) WO2011012754A2 (es)

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US20130270113A1 (en) * 2012-04-11 2013-10-17 Chuan-Hsing HUANG Electrochemical strip and manufacturing method thereof
JP5895695B2 (ja) * 2012-05-11 2016-03-30 船井電機株式会社 センサシステム及び当該センサシステムを用いた検出対象物質測定方法
GB201223166D0 (en) * 2012-12-21 2013-02-06 Alere Switzerland Gmbh Test strip
WO2015183792A1 (en) * 2014-05-27 2015-12-03 Case Western Reserve University Electrochemical sensor for analyte detection
US11009479B2 (en) 2014-05-27 2021-05-18 Case Western Reserve University Systems and methods for the detection of HbA1c
CN106687811B (zh) * 2014-07-15 2021-08-17 C2感官有限公司 用于增强化学电阻感测的制剂
JP6584821B2 (ja) * 2015-04-30 2019-10-02 株式会社東芝 測定用セル、検出装置および分析装置
KR101994370B1 (ko) * 2016-08-24 2019-06-28 주식회사 제놉시 항체 및 자성 나노 입자가 결합된 전도성 고분자를 포함하는 혈중 암세포 검출 및 회수용 자성 나노 구조체
EP3602074A1 (en) * 2017-03-22 2020-02-05 Aalto University Foundation SR Electrochemical assay for the detection of opioids
KR102465540B1 (ko) * 2017-05-18 2022-11-11 삼성전자주식회사 약액 공급 장치 및 이를 구비하는 반도체 처리 장치
IT201900011004A1 (it) * 2019-07-05 2021-01-05 Tecnosens S R L Nuovi elettrodi a matrice polimerica.
US11697807B2 (en) 2019-09-30 2023-07-11 Case Western Reserve University Electrochemical biosensor
CN112946277B (zh) * 2021-03-05 2023-12-05 山东理工大学 基于PEDOT@PSS-Pd电化学免疫传感器的制备
IT202100006260A1 (it) * 2021-03-16 2022-09-16 Enea Agenzia Naz Per Le Nuove Tecnologie Lenergia E Lo Sviluppo Economico Sostenibile Nanostrutture assemblate su elettrodi stampati.
CN114965641B (zh) * 2022-05-23 2024-05-28 可孚医疗科技股份有限公司 一种β-羟丁酸电化学试纸、制备方法及应用

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Also Published As

Publication number Publication date
WO2011012754A2 (es) 2011-02-03
US20120181173A1 (en) 2012-07-19
EP2492351A2 (en) 2012-08-29
EP2492351B1 (en) 2018-06-06

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