WO2011008231A3 - Biotransformation using genetically modified candida - Google Patents

Biotransformation using genetically modified candida Download PDF

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Publication number
WO2011008231A3
WO2011008231A3 PCT/US2010/001361 US2010001361W WO2011008231A3 WO 2011008231 A3 WO2011008231 A3 WO 2011008231A3 US 2010001361 W US2010001361 W US 2010001361W WO 2011008231 A3 WO2011008231 A3 WO 2011008231A3
Authority
WO
WIPO (PCT)
Prior art keywords
biotransformation
genetically modified
host cell
modified candida
substantially pure
Prior art date
Application number
PCT/US2010/001361
Other languages
French (fr)
Other versions
WO2011008231A2 (en
Inventor
Jon Ness
Jeremy Minshull
Original Assignee
Dna 2.0 Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/436,729 external-priority patent/US8158391B2/en
Application filed by Dna 2.0 Inc. filed Critical Dna 2.0 Inc.
Priority to EP10800135.5A priority Critical patent/EP2427559A4/en
Publication of WO2011008231A2 publication Critical patent/WO2011008231A2/en
Publication of WO2011008231A3 publication Critical patent/WO2011008231A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A substantially pure Candida host cell is provided for the biotransformation of a substrate to a product wherein the host cell is characterized by a first genetic modification class that comprises one or more genetic modifications that collectively or individually disrupt at least one alcohol dehydrogenase gene in the substantially pure Candida host cell.
PCT/US2010/001361 2009-05-06 2010-05-06 Biotransformation using genetically modified candida WO2011008231A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10800135.5A EP2427559A4 (en) 2009-05-06 2010-05-06 Biotransformation using genetically modified candida

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US17606409P 2009-05-06 2009-05-06
US12/436,729 US8158391B2 (en) 2009-05-06 2009-05-06 Production of an α-carboxyl-ω-hydroxy fatty acid using a genetically modified Candida strain
US12/436,729 2009-05-06
US61/176,064 2009-05-06

Publications (2)

Publication Number Publication Date
WO2011008231A2 WO2011008231A2 (en) 2011-01-20
WO2011008231A3 true WO2011008231A3 (en) 2011-06-16

Family

ID=43450025

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/001361 WO2011008231A2 (en) 2009-05-06 2010-05-06 Biotransformation using genetically modified candida

Country Status (2)

Country Link
EP (1) EP2427559A4 (en)
WO (1) WO2011008231A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2841794C (en) * 2011-07-06 2019-08-13 Verdezyne, Inc. Genetically modified yeast with increased alcohol dehydrogenase activity for preparing a fatty dicarboxylic acid
DE102011110946A1 (en) * 2011-08-15 2016-01-21 Evonik Degussa Gmbh Biotechnological synthesis of omega-functionalized carboxylic acids and carboxylic acid esters from simple carbon sources
CN103998410A (en) 2011-12-20 2014-08-20 帝斯曼知识产权资产管理有限公司 Process for the preparation of azeleic acid from 9-octadecenedioic acid
JP6053124B2 (en) * 2012-10-18 2016-12-27 花王株式会社 Method for producing glycolipid
CN105189731A (en) * 2012-12-19 2015-12-23 沃德金有限公司 Biological methods for preparing a fatty dicarboxylic acid
EP2746397A1 (en) * 2012-12-21 2014-06-25 Evonik Industries AG Production of omega amino fatty acids
JP6323940B2 (en) * 2013-12-26 2018-05-16 花王株式会社 Method for producing alkylpolyglycoside
KR101837130B1 (en) 2016-03-18 2018-03-09 한국생명공학연구원 Novel lipase from yeast Candida butyri SH-14 and the Use thereof
CN112746026B (en) * 2019-10-31 2023-01-10 中国石油化工股份有限公司 Candida visualis and application thereof
CN112961790B (en) * 2021-03-29 2022-11-01 江西沃邦兴环保科技有限公司 Heterotrophic nitrifying bacteria resistant to high-salt environment and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5254466A (en) * 1989-11-06 1993-10-19 Henkel Research Corporation Site-specific modification of the candida tropicals genome
US5763236A (en) * 1993-09-24 1998-06-09 Daicel Chemical Industries Ltd. Method for producing ketone or aldehyde using an alcohol dehydrogenase of Candida Parapsilosis
US20070184524A1 (en) * 2000-11-20 2007-08-09 Gokarn Ravi R 3-hydroxypropionic acid and other organic compounds

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040146999A1 (en) * 1997-07-21 2004-07-29 E.I. Du Pont De Nemours And Company Transformed yeast strains and their use for the production of monoterminal and diterminal aliphatic carboxylates

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5254466A (en) * 1989-11-06 1993-10-19 Henkel Research Corporation Site-specific modification of the candida tropicals genome
US5763236A (en) * 1993-09-24 1998-06-09 Daicel Chemical Industries Ltd. Method for producing ketone or aldehyde using an alcohol dehydrogenase of Candida Parapsilosis
US20070184524A1 (en) * 2000-11-20 2007-08-09 Gokarn Ravi R 3-hydroxypropionic acid and other organic compounds

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK [online] 18 April 2005 (2005-04-18), "C.albicans ADH1 gene for alcohol dehydrogenase 1.", XP008151005, Database accession no. X81694.1 *
MUKHERJEE ET AL.: "Alcohol Dehydrogenase Restricts the Ability of the Pathogen Candida albicans To Form a Biofilm on Catheter Surfaces through an Ethanol-Based Mechanism.", INFECT IMMUN, vol. 74, no. 7, 2006, pages 3804 - 3811, XP002543233 *
UMEMURA ET AL.: "A novel promoter, derived from the isocitrate lyase gene of Candida tropicalis, inducible with acetate in Saccharomyces cerevisiae.", APPL MICROBIOL BIOTECHNOL., vol. 43, no. 3, 1995, pages 489 - 492, XP008024452 *

Also Published As

Publication number Publication date
EP2427559A2 (en) 2012-03-14
EP2427559A4 (en) 2013-09-18
WO2011008231A2 (en) 2011-01-20

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