WO2010140795A2 - Human-placenta-like composition containing a human-placenta-derived growth factor and cytokines, and cosmetic use thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition restructured from a human-placenta-derived growth factor and cytokines. The present invention provides a cosmetic composition comprising, as active ingredients, 300 to 300,000 pg/ml of basic fibroblast growth factor-2 (FGF-2), 100 to 100,000 pg/ml of platelet-derived growth factor-AA (PDGF-AA), and 400 to 400,000 pg/ml of vascular endothelial growth factor (VEGF), and further comprising 40 to 40,000 pg/ml of Flt-3 Ligand, 300 to 300,000 pg/ml of RANTES (CCL5), 80 to 80,000 pg/ml of Interleukin-10 (IL-10), 80 to 80,0000 pg/ml of interferon-gamma-inducible protein-10 (IP-10), and 30 to 30,000 pg/ml of platelet-derived growth factor-BB (PDGF-BB), if needed. The present invention provides a cosmetic composition which exhibits excellent effects in wrinkle removal and skin-whitening even when applied in a small amount, as opposed to conventional cosmetic compositions containing placenta extracts in which unnecessary components are included.

Description

Similar placental compositions containing growth factors and cytokines derived from placenta and cosmetics thereof

The present invention relates to a cosmetic composition for the manufacture of cosmetics containing growth factors and cytokines, and more specifically to the cosmetic composition by analyzing the growth factors and cytokines derived from placenta and reconstituting the components having excellent effects on skin regeneration and whitening It relates to a cosmetic composition artificially recombined.

Skin is an indispensable organ in our lives and one of the most vulnerable organs in the human body. Skin aging can be divided into two categories, internal aging and external aging, depending on the cause. Intrinsic aging is an aging process that occurs naturally as the body ages and the physiological function of the body decreases. Extrinsic aging refers to aging caused by external factors such as ultraviolet (UV), dry air, reactive oxygen species and stress. As the skin ages due to these internal and external factors, the most common symptom is wrinkle formation on the skin surface.

There are many known pathways for generating skin wrinkles. For example, when the skin is exposed to excessive ultraviolet light, collagen breakdown, which is one of the main components of the skin, is promoted, causing the skin to lose elasticity and wrinkles. When the skin is dried due to temperature change, humidity decrease or wind, the skin function as a protective barrier against the outside is degraded and wrinkles are generated. Furthermore, when the skin is exposed to noxious reactive oxygen species or free radicals, lipid peroxide may be produced by oxidative action, and skin constituent proteins such as collagen may be modified to produce wrinkles. As the skin ages, keratinization and epidermal cell fusion rate gradually slows down, the epidermal cell layer becomes thin, the stratum corneum becomes thick, and the boundary line with the dermis layer becomes straight. In the dermis, human skin fibroblasts age, reducing the ability to produce fibers and substrates. In addition, as the skin cell ages, the peroxide level in the cell increases rapidly and is affected by various pollutants and stress. If the constituents of the cell (or cell membrane) are changed to peroxides and protein damage occurs, the cell (or cell membrane) is unable to perform its function smoothly, which lowers the metabolic activity of the cell, thus causing wrinkles due to functional and structural damage. These promote skin aging. Due to skin damage caused by such external environment or internal mechanism, various studies have been made in the cosmetic manufacturing industry to suppress the appearance of skin aging or wrinkles.

Placenta, on the other hand, is an organ that develops in the womb of a pregnant mammal. Placenta has the effect of inhibiting the activity of the enzyme tyrosinase, which catalyzes the production of melanin granules from tyrosine, thereby inhibiting the production of melanin pigment and promoting the release of cells containing melanin pigment, thus preventing and improving blemishes. It is effective in preventing and causing pigmentation as well as whitening (Mallick S et al., Pigment Cell Res. Feb 18 (1), 25-33, 2005). Placenta contains essential amino acids, melatonin, RNA, DNA and other nucleic acid components, antioxidant enzymes, hyaluronic acid, antioxidants, immune cofactors (cytokine), placental peptides, insulin-like-growth factor ), Growth factors such as epidermal growth factors (EGFS) and unique senescent cell activating factors (SCAFS) and cytokines are known to be useful for fatigue and immune augmentation.

Various methods for preparing cosmetics using such placental extracts have been disclosed. Japanese Patent Application Laid-Open No. 35-15399 discloses a color white cosmetic compound comprising placenta extract and koji acid or koji acid derivative in a cosmetic base. Korean Patent Laid-Open Publication No. 2004-0082144 discloses a skin coating composition comprising the addition of organic germanium and ubiquinone to a placental extract. Republic of Korea Patent Publication No. 2008-0096219 discloses a cosmetic raw material to increase the amino acid content by treating proteolytic enzymes in pig placenta.

However, these inventions can be extracted directly from the mammalian placenta including humans and used as a cosmetic raw material, according to the extraction method may contain a large amount of impurities not involved in skin care, immune rejection in case of skin in particular skin wounds There is a risk of causing a reaction, and since it is extracted from nature, there is a restriction on supply and demand of raw materials, especially in the case of placenta, the sale of cosmetics containing the placenta extract is banned for reasons of ethical issues.

The present invention is to solve the above-mentioned problems, instead of using the placenta extract as a direct cosmetic raw material, extracting and analyzing various growth factors and cytokines derived from the placenta, and then effective in improving wrinkles and skin whitening from these By formulating ingredients and providing the most effective growth factor and cytokine ingredient combinations for improving skin wrinkles and skin whitening from various combinations of these ingredients as cosmetic raw materials, there is no fear of ethical problems occurring, Unlike the placenta extract cosmetic composition, its purpose is to provide a cosmetic composition having an excellent effect on wrinkle improvement and skin whitening even in a small amount.

In order to achieve the above object, the present inventors obtain extracts of various components from the placenta, analyze various growth factors and cytokines from these extracts, derive various combinations from these growth factors and cytokines, and reconstruct each of the components. As a result of preparing and experimenting with the composition, the present invention was found to have an excellent effect on wrinkle improvement and skin whitening in combination of certain components.

Basic Fibroblast Growth Factor-2 (FGF-2) 300-300,000 pg / ml, Platelet-derived growth factor-AA (PDGF-AA) 100-100,000 pg / ml, vascular endothelial growth factor (Vascular endothelial growth factor, VEGF) provides a cosmetic composition for preparing a cosmetic comprising as an active ingredient 400-400,000 pg / ml.

In addition, the present invention provides a cosmetic composition further comprises 40-40,000 pg / ml Flt-3 ligand (Flt-3 Ligand) in the active ingredients.

In addition, the present invention provides a cosmetic composition further comprises 300-300,000 pg / ml of Lantis (RANTES, CCL5) to the active ingredients.

In addition, the present invention is an interleukin-10 (Interleukin-10, IL-10) 80-80,000 pg / ml or Interferon-γ-inducing factor-10 (Interferon gamma inducible protein-10, IP-10) 80- It provides a cosmetic composition further comprises one or more selected from the group consisting of 80,0000 pg / ml, Platelet-derived growth factor-BB (PDGF-BB) 30-30,000 pg / ml.

The cosmetic composition of the present invention, unlike the conventional cosmetic composition using the placenta extract as a direct cosmetic raw material, by analyzing the placenta extract to find growth factors and cytokines effective for wrinkle improvement and skin whitening, and then improve the skin wrinkles from a combination of these components By providing it as the most effective cosmetic raw material for skin whitening, there is no fear of occurrence of ethical problems, and unlike the conventional placenta extract cosmetic composition which contained unnecessary ingredients, a cosmetic composition having an excellent effect on wrinkle improvement and skin whitening even with a small amount Has the effect of providing.

1 is a table showing the ingredient names and effective substance content of growth factors and cytokines analyzed by using the placenta extract of the present invention using multiplex cytokine array.

Hereinafter, the cosmetic composition of the present invention through the examples will be described in detail. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example 1

(A) Extracting Active Ingredients from Placenta

The human placenta is washed, throttled, broken and lyophilized in vacuo to lower the water content to less than 10%. Next, 100 g of powdered placenta and supercritical solvent in the supercritical extractor reactor were dissolved in carbon dioxide into the extraction reactor, and the temperature in the reactor was adjusted to 35 ° C., and extracted for about 45 minutes while maintaining the pressure at 3000 psi. Insoluble material was collected in a separation tank, and soluble material was returned to its original state to gasification of carbon dioxide to obtain a placental extract.

(B) Analysis of active ingredients effective for wrinkle improvement and whitening

Table 1 shows the ingredient names and effective substance contents of growth factors and cytokines effective for wrinkle improvement and skin whitening by analyzing the active ingredients of the placenta extract obtained by the method (A) using a multiplex cytokine array.

As shown in FIG. 1, FGF-2, PDGF-AA, VEGF, Flt-3 Ligand, RANTES, IL-10, IP-10, PDGF-BB, etc. are found to act as active ingredients of the placenta extract. lost.

Example 2

(1) Basic fibroblast growth factor (FGF-2) 3,000 pg / ml, Platelet-derived growth factor-AA (PDGF-AA) 1,000 pg / ml, Vascular endothelial 4,000 pg / ml of growth factor (Vascular endothelial growth factor, VEGF) was mixed to prepare a cosmetic composition of the present invention.

(2) 3.05% by weight of the cosmetic composition, 0.45% by weight of carboxyvinyl polymer, 7.0% by weight of squalane, 5.0% by weight of concentrated glycerin, 0.8% by weight of triethanolamine, 1.5% by weight of glyceryl stearate, cetyl alcohol 2.0 wt%, isocetyloctanoate 4.0 wt%, beeswax 0.5 wt%, polysorbate 60 0.5 wt%, xanthan gum 0.05 wt%, Fiji-80 Glyceryl Cocoate 1.0 wt%, sodium coco ampopropio Nate 4.0% by weight, an appropriate amount of preservatives, fragrances, pigments and purified water were added and stirred sufficiently to prepare a makeup cream.

Example 3

(1) 400 pg / ml of Flt-3 Ligand was further mixed with the cosmetic composition of Example 2 to prepare a cosmetic composition of the present invention.

(2) A cosmetic cream was prepared in the same manner as in Example 2.

Example 4

(1) The cosmetic composition of Example 3 was further mixed with 3,000 pg / ml of Lantis (RANTES, CCL5) to the cosmetic composition of Example 3.

(2) A cosmetic cream was prepared in the same manner as in Example 2.

Example 5

(1) In the cosmetic composition of Example 4, 800 pg / ml of Interleukin-10 (IL-10), Interferon-γ-inducing factor-10 (800-10 pg) / ml, platelet-derived growth factor-BB (Platelet-derived growth factor-BB, PDGF-BB) 30-0 pg / ml was further mixed to prepare a cosmetic composition of the present invention.

(2) A cosmetic cream was prepared in the same manner as in Example 2.

Comparative Example 1

0.45% by weight of carboxyvinyl polymer, 7.0% by weight of squalane, 5.0% by weight of concentrated glycerin, 0.8% by weight of triethanolamine, 1.5% by weight of glyceryl stearate, and 2.0% of cetyl alcohol, except for the cosmetic composition of the present invention. %, Isocetyloctanoate 4.0 wt%, beeswax 0.5 wt%, polysorbate 60 0.5 wt%, xanthan gum 0.05 wt%, Fiji-80 glycerol cocoate 1.0 wt%, sodium coco ampopropionate 4.0 A cosmetic cream was prepared by sufficiently stirring the weight%, an appropriate amount of preservative, a perfume, a pigment, and purified water.

Experimental Example 1

In order to examine the wrinkle improvement effect of the makeup creams prepared in Examples 2 to 5, 10 women in their 30s to 40s were used as the same experimental group, and the makeup creams of this example and the comparative example were applied twice a day to the wrinkles around the eyes. After applying for 1 month, the wrinkle improvement state was visually observed and is shown in Table 1 below.

Table 1

division	No improvement	Minor improvements	Improvement in severity	A significant improvement
Example 2	-	One	4	5
Example 3	-	2	2	6
Example 4	-	-	2	8
Example 5	-	-	One	9
Comparative Example 1	7	3	-	-

As shown in Table 1, the makeup cream according to the embodiment of the present invention showed an excellent effect on wrinkle improvement, the case of Example 5 showed the most excellent wrinkle improvement effect.

The present invention relates to a cosmetic composition for producing cosmetics containing growth factors and cytokines. It relates to a cosmetic composition artificially recombining the cosmetic composition by reconstituting the components having excellent effects on skin regeneration and whitening by analyzing growth factors and cytokines derived from the placenta.

Claims (8)

1. Basic fibroblast growth factor (FGF-2) 300-300,000 pg / ml, Platelet-derived growth factor-AA (PDGF-AA) 100-100,000 pg / ml Vascular endothelial growth factor (VEGF) cosmetic composition for the manufacture of cosmetics comprising 400-400,000 pg / ml as an active ingredient.
2. The cosmetic composition of claim 1, further comprising 40-40,000 pg / ml of Flt-3 Ligand.
3. The cosmetic composition according to claim 1, further comprising 300-300,000 pg / ml of Lantis (RANTES, CCL5).
4. The cosmetic composition according to claim 2, further comprising 300-300,000 pg / ml of Lantis (RANTES, CCL5).
5. According to claim 1, Interleukin-10 (IL-10) 80-80,000 pg / ml or Interferon-γ-inducing factor-10 (Interferon gamma inducible protein-10, IP-10) 80-80,0000 pg / ml, platelet-derived growth factor-BB (Platelet-derived growth factor-BB, PDGF-BB) 30-30,000 pg / ml cosmetic composition further comprises one or more selected from the group consisting of.
6. The method of claim 2, wherein Interleukin-10 (IL-10) 80-80,000 pg / ml or Interferon gamma inducible protein-10 (IP-10) 80-80,0000 pg / ml, platelet-derived growth factor-BB (Platelet-derived growth factor-BB, PDGF-BB) 30-30,000 pg / ml cosmetic composition further comprises one or more selected from the group consisting of.
7. According to claim 3, Interleukin-10 (IL-10) 80-80,000 pg / ml or Interferon-γ-inducing factor-10 (Interferon gamma inducible protein-10, IP-10) 80-80,0000 pg / ml, platelet-derived growth factor-BB (Platelet-derived growth factor-BB, PDGF-BB) 30-30,000 pg / ml cosmetic composition further comprises one or more selected from the group consisting of.
8. According to claim 3, Interleukin-10 (IL-10) 80-80,000 pg / ml or Interferon-γ-inducing factor-10 (Interferon gamma inducible protein-10, IP-10) 80-80,0000 pg / ml, platelet-derived growth factor-BB (Platelet-derived growth factor-BB, PDGF-BB) 30-30,000 pg / ml cosmetic composition further comprises one or more selected from the group consisting of.

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