WO2010134499A3 - リステリア菌検出用のプライマーセット - Google Patents

リステリア菌検出用のプライマーセット Download PDF

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Publication number
WO2010134499A3
WO2010134499A3 PCT/JP2010/058305 JP2010058305W WO2010134499A3 WO 2010134499 A3 WO2010134499 A3 WO 2010134499A3 JP 2010058305 W JP2010058305 W JP 2010058305W WO 2010134499 A3 WO2010134499 A3 WO 2010134499A3
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WO
WIPO (PCT)
Prior art keywords
gene
seq
base sequence
sequence represented
primer set
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Application number
PCT/JP2010/058305
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English (en)
French (fr)
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WO2010134499A2 (ja
Inventor
宮本　敬久
田中　孝
Original Assignee
国立大学法人 九州大学
明治乳業株式会社
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Application filed by 国立大学法人 九州大学, 明治乳業株式会社 filed Critical 国立大学法人 九州大学
Publication of WO2010134499A2 publication Critical patent/WO2010134499A2/ja
Publication of WO2010134499A3 publication Critical patent/WO2010134499A3/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　特に高い病原性を有するListeria monocytogenesの菌株を特異的かつ迅速に検出、同定する方法を提供する。　Listeria monocytogenesが有するｈｌｙＡ遺伝子、ｉｎｌＡ遺伝子、ｃｌｐＣ遺伝子、ｐｌｃＡ遺伝子の一塩基多型によって分類し、各遺伝子内の一塩基多型に特異的な一塩基置換若しくは分類可能に特定された組み合わせからなる複数の一塩基多型を検出することにより、臨床株で見出された菌株と同じグループに属する病原性の高い菌株を検出する。このために、例えば、配列番号１に示す塩基配列を有するプライマーと配列番号１２～１６及び１８～２０に示す何れかの塩基配列を有するプライマーとからなるプライマーセット、若しくは配列番号１１に示す塩基配列を有するプライマーと配列番号４の塩基配列を有するプライマーとからなるプライマーセットを用いてＰＣＲ法を適用する。
PCT/JP2010/058305 2009-05-18 2010-05-17 リステリア菌検出用のプライマーセット WO2010134499A2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2009-120420 2009-05-18
JP2009120420A JP5522623B2 (ja) 2009-05-18 2009-05-18 リステリア菌検出用のプライマーセット

Publications (2)

Publication Number Publication Date
WO2010134499A2 WO2010134499A2 (ja) 2010-11-25
WO2010134499A3 true WO2010134499A3 (ja) 2011-03-10

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PCT/JP2010/058305 WO2010134499A2 (ja) 2009-05-18 2010-05-17 リステリア菌検出用のプライマーセット

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JP (1) JP5522623B2 (ja)
WO (1) WO2010134499A2 (ja)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110592189B (zh) * 2019-09-26 2022-05-17 北京市食品安全监控和风险评估中心（北京市食品检验所） 一种单核细胞增生李斯特氏菌的多位点基因分型方法
CN112011537B (zh) * 2020-09-24 2022-11-04 上海国际旅行卫生保健中心(上海海关口岸门诊部) 一组扩增李斯特氏菌mlst分型溯源的管家基因的引物组及二代测序建库方法和应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007117022A (ja) * 2005-10-28 2007-05-17 Kirin Brewery Co Ltd リステリア・モノサイトゲネス検出のためのプライマーセットおよび方法
JP2007274934A (ja) * 2006-04-04 2007-10-25 Nippon Meat Packers Inc プライマーセット及び食中毒細菌の検出方法
JP2007312660A (ja) * 2006-05-24 2007-12-06 Kyushu Univ リステリア菌の検出・同定方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007117022A (ja) * 2005-10-28 2007-05-17 Kirin Brewery Co Ltd リステリア・モノサイトゲネス検出のためのプライマーセットおよび方法
JP2007274934A (ja) * 2006-04-04 2007-10-25 Nippon Meat Packers Inc プライマーセット及び食中毒細菌の検出方法
JP2007312660A (ja) * 2006-05-24 2007-12-06 Kyushu Univ リステリア菌の検出・同定方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ANGELIKA LEHNER ET AL.: "A rapid differentiation of Listeria monocytogenes by use of PCR-SSCP in the listeriolysin 0 (hlyA) locus", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 34, 1999, pages 165 - 171 *
KEN-ICHI HONJOH ET AL.: "Subtyping of Listeria monocytogenes Based on Nucleotide Polymorphism in the clpC, inlA, hlyA, and plcA Genes and Rapid Identification of L. monocytogenes Genetically Similar to Clinical Isolates", FOOD SCI. TECHNOL. RES., vol. 14, no. 6, 2008, pages 557 - 564 *
TAKAHISA MIYAMOTO ET AL.: "Listeria monocytogenes Rinsho Yurai Kabu Tokuiteki na SNP Kenshutsuho", DAI 33 KAI NENJI TAIKAI YOSHISHU, 29 May 2006 (2006-05-29), pages 62 *

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Publication number Publication date
JP5522623B2 (ja) 2014-06-18
WO2010134499A2 (ja) 2010-11-25
JP2010263873A (ja) 2010-11-25

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