WO2010129441A3 - Procédé de séparation de cellules souches à partir de leur descendance plus différenciée à l'aide de dispositifs microfluidiques - Google Patents

Procédé de séparation de cellules souches à partir de leur descendance plus différenciée à l'aide de dispositifs microfluidiques Download PDF

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Publication number
WO2010129441A3
WO2010129441A3 PCT/US2010/033298 US2010033298W WO2010129441A3 WO 2010129441 A3 WO2010129441 A3 WO 2010129441A3 US 2010033298 W US2010033298 W US 2010033298W WO 2010129441 A3 WO2010129441 A3 WO 2010129441A3
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WO
WIPO (PCT)
Prior art keywords
stem cells
microfluidic devices
differentiated progeny
separating stem
separating
Prior art date
Application number
PCT/US2010/033298
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English (en)
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WO2010129441A2 (fr
Inventor
Michael Grisham
Original Assignee
Gpb Scientific, Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Gpb Scientific, Llc filed Critical Gpb Scientific, Llc
Publication of WO2010129441A2 publication Critical patent/WO2010129441A2/fr
Publication of WO2010129441A3 publication Critical patent/WO2010129441A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention porte sur des procédés et des dispositifs pour séparer des cellules. Les procédés et appareils permettent l'élimination de cellules différenciées ou plus engagées à partir de cultures de cellules souches.
PCT/US2010/033298 2009-05-04 2010-04-30 Procédé de séparation de cellules souches à partir de leur descendance plus différenciée à l'aide de dispositifs microfluidiques WO2010129441A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17541809P 2009-05-04 2009-05-04
US61/175,418 2009-05-04

Publications (2)

Publication Number Publication Date
WO2010129441A2 WO2010129441A2 (fr) 2010-11-11
WO2010129441A3 true WO2010129441A3 (fr) 2011-04-21

Family

ID=43050789

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/033298 WO2010129441A2 (fr) 2009-05-04 2010-04-30 Procédé de séparation de cellules souches à partir de leur descendance plus différenciée à l'aide de dispositifs microfluidiques

Country Status (1)

Country Link
WO (1) WO2010129441A2 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11243494B2 (en) 2002-07-31 2022-02-08 Abs Global, Inc. Multiple laminar flow-based particle and cellular separation with laser steering
WO2004029221A2 (fr) 2002-09-27 2004-04-08 The General Hospital Corporation Dispositif microfluidique pour la separation de cellules et utilisations de ce dispositif
US10908066B2 (en) 2010-11-16 2021-02-02 1087 Systems, Inc. Use of vibrational spectroscopy for microfluidic liquid measurement
CN103146650B (zh) * 2013-02-23 2015-06-10 大连理工大学 基于微流控技术的两步构建三维神经干细胞模型的方法
US9631179B2 (en) 2013-03-15 2017-04-25 Angle North America, Inc. Methods for segregating particles using an apparatus with a size-discriminating separation element having an elongate leading edge
EP2971287B1 (fr) * 2013-03-15 2019-08-14 GPB Scientific, LLC Traitement microfluidique intégré de particules
EP3608022A1 (fr) * 2013-03-15 2020-02-12 The Trustees of Princeton University Procédés et dispositifs de purification à haut rendement
US20150064153A1 (en) 2013-03-15 2015-03-05 The Trustees Of Princeton University High efficiency microfluidic purification of stem cells to improve transplants
US8961904B2 (en) 2013-07-16 2015-02-24 Premium Genetics (Uk) Ltd. Microfluidic chip
JP6403190B2 (ja) * 2013-08-06 2018-10-10 旭化成株式会社 マイクロ流路構造体及び粒子の分離方法
US11796449B2 (en) 2013-10-30 2023-10-24 Abs Global, Inc. Microfluidic system and method with focused energy apparatus
US10976232B2 (en) 2015-08-24 2021-04-13 Gpb Scientific, Inc. Methods and devices for multi-step cell purification and concentration
CN111065399B (zh) 2017-09-01 2023-11-28 Gpb科学有限公司 使用微流体制备治疗活性细胞的方法
CN117721078A (zh) * 2018-05-13 2024-03-19 Gpb科学有限公司 通过确定性侧向位移与产物再循环的组合的纯化和浓缩
US11331670B2 (en) 2018-05-23 2022-05-17 Abs Global, Inc. Systems and methods for particle focusing in microchannels
BR112021020390A2 (pt) 2019-04-18 2022-01-18 Abs Global Inc Sistema de distribuição de crioprotetor, sistema de criopreservação para distribuir um crioprotetor a um espécime biológico, método para distribuir um crioprotetor a um espécime biológico, sistema de distribuição e método para preparar um espécime biológico para criopreservação
US11628439B2 (en) 2020-01-13 2023-04-18 Abs Global, Inc. Single-sheath microfluidic chip

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6685841B2 (en) * 2001-02-14 2004-02-03 Gabriel P. Lopez Nanostructured devices for separation and analysis
US20060134599A1 (en) * 2002-09-27 2006-06-22 Mehmet Toner Microfluidic device for cell separation and uses thereof
US7150812B2 (en) * 2002-10-23 2006-12-19 The Trustees Of Princeton University Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields
US20070026414A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6685841B2 (en) * 2001-02-14 2004-02-03 Gabriel P. Lopez Nanostructured devices for separation and analysis
US20060134599A1 (en) * 2002-09-27 2006-06-22 Mehmet Toner Microfluidic device for cell separation and uses thereof
US7150812B2 (en) * 2002-10-23 2006-12-19 The Trustees Of Princeton University Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields
US20070026414A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NATHAN BLOW: "Microfluidics: the great divide", NATURE METHODS, vol. 6, no. 9, September 2009 (2009-09-01), pages 683 - 686 *
STEFAN KOBEL ET AL.: "High-throughput methods to define complex stem cell niches", BIOTECHNIQUES., vol. 48, no. 4, April 2010 (2010-04-01), pages IX - XXII *

Also Published As

Publication number Publication date
WO2010129441A2 (fr) 2010-11-11

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