WO2010108314A1

WO2010108314A1 - Use of fiber pentose or composition containing fiber pentose in manufacture of foods or medicines for preventing and/or treating acidification in diabetic patient

Info

Publication number: WO2010108314A1
Application number: PCT/CN2009/070959
Authority: WO
Inventors: 孙萌; 朱作霖; 苏春高; 叶红平
Original assignee: 淮北中润生物能源技术开发有限公司; 美国升阳制药公司
Priority date: 2009-03-24
Filing date: 2009-03-24
Publication date: 2010-09-30

Abstract

Fiber pentose or a composition containing fiber pentose is used in the manufacture of foods or medicines for preventing and/or treating acidification of the body in diabetic patients. The composition comprises fiber pentose and substances that promote absorption in the body of fiber pentose. The substances that promote absorption of fiber pentose are selected from cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, adrenalin, potassium ion, magnesium ion and sodium ion. The fiber pentose is xylose, ribose, arabinose, lyxose, ribulose and/or xylulose. The fiber pentose is prepared by mixing cellulose-containing biomass, water and catalyst, hydrolyzing and then fermenting with yeast. The catalyst is selected from hemicellulose hydrolase, organic acid, inorganic acid or biomimetic catalyst.

Description

Use of a fiber pentose or a fiber pentose-containing composition for the preparation of a food or a medicament for preventing and/or treating acidification of a body of a diabetic patient

The present invention relates to the field of cellulosic biomass, and more particularly to a composition for controlling acidification of a body of a diabetic patient. Background technique

Diabetes is a disease in which the body cannot secrete insulin, or the amount of insulin secreted or/and the quality of insulin is insufficient, causing the body to be unable to effectively degrade metabolic glucose. Currently, people with diabetes generally use more protein and so on to provide the body with the necessary energy supply. Since the metabolism of substances such as proteins is not the most suitable way to supply energy to the body, a large amount of this metabolism causes incomplete metabolism, and since most protein molecules contain many heteroatoms such as nitrogen, sulfur, and phosphorus, etc., The cumulative result is the acidification of the body.

Acidification of the body is a common phenomenon in patients with diseases and the elderly. The bodies of healthy people and adolescents are all slightly alkaline. In an acidic environment, many high-activity particles such as free radicals are more easily produced, which raises the body's oxidative stress level, and abnormal oxidative stress is the most fundamental cause of various cell damage and death in the body, abnormal levels. Oxidative stress is thought to be an important cause of chronic complications of diabetes and diabetes, pulmonary fibrosis, epilepsy, hypertension, atherosclerosis and corresponding cardiovascular diseases, cancer, nephropathy, and Parkinson's disease. According to the clinical evaluation results, many patients, such as cancer patients, are basically acidic, maintaining the body in a slightly alkaline state, which is one of the important means to reduce the level of oxidative stress.

The acidity and alkalinity of foods are not determined by mouthfeel, but by the results of digestion and absorption of food in the body. If there are more anions such as phosphate, sulfate, nitrate, and chloride ions produced after food metabolism, it will cause acidification of the body and be an acidic food. If the food is metabolized, sodium ions, potassium ions, magnesium ions, and calcium are produced. If there are many ionic plasmas, it is easy to cause alkalization of the body and it is an alkaline food. Although citric acid and so on are acidic, human metabolism converts it into water and carbon dioxide. It is a neutral food. Protein has no acid-base taste, but the body metabolizes it to produce anions such as sulfate, nitrate and chloride. It is an acidic food; the final metabolites of carbohydrates are water and carbon dioxide. It can be seen that when a diabetic patient becomes a subject, the body of the food is converted from carbohydrate to protein, and the body is highly susceptible to acidification, resulting in a further increase in oxidative stress.

The metabolism of natural five-carbon sugars does not require the participation of insulin, and their sweetness is similar to that of glucose. Therefore, natural five-carbon sugar is an ideal glucose replacement sugar, a substance that provides sugar source energy for diabetic patients and restores diabetes. Eating habits can eat sugar. However, their use in life is limited for two reasons. First, the degree of absorption of five carbon sugars in the body is low, It is because their production costs are too high and most people are unlikely to consume.

Therefore, there is an urgent need in the art to provide a substance containing natural five-carbon sugar, which has a high degree of absorption in the body, can effectively prevent acidification of a body of a diabetic patient, and has low production cost. Summary of the invention

The present invention is directed to the use of a fiber pentose or a composition comprising a fiber pentose.

Another object of the present invention is to provide a process for preparing pure xylose from cellulosic biomass. In a first aspect of the invention, there is provided a use of a pentose pentose or a pentose-containing composition for the preparation of a food or medicament for preventing and/or treating acidification of a body of a diabetic patient.

In another preferred embodiment, the fiber pentose-containing composition is composed of a fiber pentose sugar and a substance which promotes absorption of the fiber pentose sugar in the body.

In another preferred embodiment, based on 100 parts by weight of the composition, it contains:

1 100 parts by weight of pentose sugar; and

0-99 parts by weight of a sugar absorption enhancer; the sugar absorption enhancer is selected from one or more of the following: cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, adrenaline, potassium ion, Magnesium ions, sodium ions.

In another preferred embodiment, it contains: 100 parts by weight of pentose sugar; and 0.001 - 99 parts by weight of a sugar absorption enhancer, based on 100 parts by weight of the composition.

In another preferred embodiment, the weight ratio of the pentose sugar to the sugar absorption enhancer is 0.001.

1000:1, preferably 0.015 - 10:1, more preferably 0.02 - 0.7:1.

In another preferred embodiment, the substance for promoting absorption of the fiber pentose in the body includes all substances which promote the absorption of the fiber pentose in the body, and may be a macromolecular substance, a small molecule substance or even an ion. , including but not limited to cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, adrenaline, potassium, magnesium, sodium, and the like.

In another preferred embodiment, the cellulose comprises all of the cellulose, preferably microcrystalline cellulose, most preferably nano-sized cellulose.

In another preferred embodiment, the fiber pentose sugar is selected from one or more of the following: xylose, ribose, arabinose, lyxose, nucleoside Ribulose, xylulose.

In another preferred embodiment, the fiber pentose is obtained by the following steps:

(a) mixing the biomass containing cellulose with water and hydrolyzing at 20-18 CTC to obtain liquid mixture I;

(b) After mixing the liquid mixture I and the alcohol yeast at 20 to 60 ° C for 6 to 48 hours, the solid matter was filtered off and concentrated to obtain a fiber pentose. In another preferred embodiment, the fiber pentose is obtained by the following steps:

(1) The cellulose-containing biomass, water and catalyst are mixed at 30-18 CTC for 3 to 600 minutes, and hydrolyzed to obtain a liquid mixture I; the catalyst is selected from the group consisting of: hemicellulose hydrolase, organic acid, inorganic acid, or a biomimetic catalyst; the organic acid is selected from the group consisting of: an organic acid containing less than 20 carbons; the inorganic acid is selected from the group consisting of: sulfuric acid, hydrochloric acid, and phosphoric acid; and the biomimetic catalyst is selected from the group consisting of [CpFeCO (maleic acid)] BF4 Or iron bis(diamine diacetate) complex;

(2) mixing liquid mixture I and alcohol yeast at 20-60 ° C for 6 to 48 hours to obtain a liquid mixture Π;

(3) The liquid mixture II is ion-exchanged to obtain a fiber pentose.

In another preferred embodiment, the fiber pentose contains 1 to 90% by weight of arabinose, based on the total weight of the fiber pentose _; preferably, the fiber pentose contains 5-25% by weight of the arabinose.

(a) mixing the cellulose-containing biomass with water and hydrolyzing at 90-18 CTC to obtain liquid mixture I;

(b) Mixing the liquid mixture I and the alcohol yeast at 20-40 ° C for 12 to 48 hours to obtain a cellulose pentose.

(1) The cellulose-containing biomass, water and catalyst are mixed at 90-18 CTC for 30-120 minutes, and hydrolyzed to obtain a liquid mixture I; the catalyst is selected from the group consisting of: hemicellulose hydrolase, organic acid, inorganic acid, or a biomimetic catalyst; the organic acid is selected from the group consisting of: an organic acid containing less than 20 carbons; the inorganic acid is selected from the group consisting of: sulfuric acid, hydrochloric acid, and phosphoric acid; and the biomimetic catalyst is selected from the group consisting of: [CpFeCO (maleic acid)] BF4, or iron diammonium diacetate complex;

(2) mixing liquid mixture I and alcohol yeast at 20-40 ° C for 12 to 48 hours to obtain a liquid mixture Π;

(3) The liquid mixture II is ion-exchanged to obtain a fiber pentose.

In another preferred embodiment, the food product comprises a solid food and a liquid food. In a second aspect of the invention, there is provided a method of preparing pure xylose from cellulosic biomass, the method comprising the steps of: recrystallizing a fiber pentose sugar through a simple ethanol/water mixed solvent; Pentose is obtained by the following steps:

(1) The cellulose-containing biomass, water and catalyst are mixed at 90-18 CTC for 30-120 minutes, and hydrolyzed to obtain a liquid mixture I; the catalyst is selected from the group consisting of: hemicellulose hydrolase, organic acid, inorganic acid, or a biomimetic catalyst; the organic acid is selected from the group consisting of: an organic acid containing less than 20 carbons; the inorganic acid is selected from the group consisting of: sulfuric acid, hydrochloric acid, and phosphoric acid; and the biomimetic catalyst is selected from the group consisting of: [CpFeCO (maleic acid)] BF4, or iron diammonium diacetate complex; (2) mixing the liquid mixture I and the alcohol yeast at 20-40 ° C for 12 to 48 hours to obtain a liquid mixture Π;

(3) The liquid mixture I I is ion-exchanged to obtain a fiber pentose. Accordingly, the present invention provides a substance containing natural five-carbon sugar, which has a high degree of absorption in the body, can effectively prevent acidification of a body of a diabetic patient, and has low production cost. detailed description

After extensive and in-depth research, the inventors found that by refining cellulose-containing biomass, a product of natural five-carbon sugar can be obtained: Fiber pentose, when mixed with other substances, can greatly enhance natural five-carbon sugar in the body. The effective absorption can provide a source of sugar energy for diabetic patients, preventing the acidification of the body caused by long-term use of protein as an energy substance in diabetic patients, thereby reducing oxidative stress and preventing diseases. Definition

As used herein, "cellulose-containing biomass" and "cellulose-containing biomass" are used interchangeably and refer to all cellulose-containing materials, including cellulose biomass in the traditional definition, including plants. The seeds, fruits, stems, leaves, and roots even include some microorganisms. The plants include all non-toxic plants, and the properties of the product are not affected by the source of the plant. All cellulose-containing biomass may be used, preferably a hemicellulose content of 5% by weight or more based on the total weight of the cellulosic biomass, most preferably a hemicellulose content of 10 wt% based on the total weight of the cellulosic biomass. More than % of the substance.

As used herein, "cellulosic biomass" refers to biomass containing cellulose, hemicellulose, and lignin as a major component, and generally does not include the fruits of plants.

Microcrystalline cellulose is a pure natural cellulose depolymerization product, which is in the form of fine powder, white, odorless, tasteless; composed of free-flowing crystalline powder (non-fibrous particles). Insoluble in water, diluted acid and most organic solvents, slightly soluble in 20% alkali solution. It can be directly used for dry powder tableting, and is widely used as a pharmaceutical excipient, a flow aid, a filler, a disintegrant, an anti-adhesive agent, an adsorbent, a capsule diluent, and the like. Nanocrystalline cellulose in the present invention refers to a cellulosic material having a cross-sectional length of 10 to 20 nm and a fiber length of less than 100 nm.

As used herein, the fiber pentose sugar is selected from all natural five carbon sugars, such as xylose, ribose, arab inose, lyxose, and pentoketone. Sugar ribulose (r ibulose), xylulose, xylulose, and the like. The pentose sugar may be either a single pure five-carbon sugar or a mixture thereof, and the content of each sugar in the mixture is not limited. In the present invention, "fibrous pentose", "natural five-carbon sugar composition" or "natural five-carbon sugar" can be interchanged. Use, all refer to all naturally occurring monosaccharides containing five carbon atoms in nature, or simply derivatives or polymers thereof.

As used herein, "mimetic catalyst" refers to a synthetic catalyst containing at least one catalytic domain and a cel lulose binding domain, such as [CpFeCO (maleic acid)] BF4, Iron complex of ethylene diamine diacetate, and the like. The biomimetic catalyst is described in detail in the patent application PCT/CN/2007/071114, the entire disclosure of which is incorporated herein by reference. In another aspect, the pentose sugar of the present invention can be obtained by the following steps:

(a) mixing the cellulose-containing biomass with water and hydrolyzing at 20-18 CTC to obtain liquid mixture I;

(b) The liquid mixture I and the alcohol yeast are mixed at 20 to 60 ° C for 6 to 48 hours, the solid matter is filtered off, and concentrated to obtain a fiber pentose.

In a preferred embodiment of the invention, the fiber pentose is obtained by the following steps:

In the first step, the pulverized cellulose-containing biomass is dewaxed using near-boiling water (80-98 ° C, preferably 90-95 ° C);

In the second step, the dewaxed cellulose-containing biomass and catalyst are mixed at 30-14 CTC for 3 - 600 minutes, hydrolyzed, and solid-liquid separated to obtain a liquid mixture I; the catalyst is selected from the group consisting of: hemicellulose hydrolase The organic acid, the inorganic acid, or the biomimetic catalyst; the organic acid is selected from the group consisting of: an organic acid containing less than 20 carbons; the inorganic acid is selected from the group consisting of: sulfuric acid, hydrochloric acid, and phosphoric acid; and the biomimetic catalyst is selected from the group consisting of: Patent application PCT/CN/2007/071114;

In the third step, the catalyst is separated or inactivated; the separation comprises passing the liquid mixture I through the macroporous adsorption resin; the inactivation comprises heating, neutralization, etc.;

In the fourth step, the liquid mixture I and the alcohol yeast are mixed at 20-60 ° C for 6 to 48 hours, and the solid is filtered to obtain a liquid product Π;

In the fifth step, the liquid product I I is subjected to ion exchange to obtain a fiber pentose solution.

The ion exchange in the fifth step includes cation exchange, and anion exchange, and sometimes in order to improve the quality of the product, a mixed column containing both a cationic resin and an anionic resin is added. Elution Generally, pure water is used, and the regeneration of the exchange resin is generally an aqueous solution of sodium hydroxide and dilute hydrochloric acid. The composition for preventing acidification of a body of a diabetic patient provided by the invention comprises a fiber pentose sugar and a pentose sugar absorption enhancer; wherein the sugar absorption enhancer is selected from one or more of the following substances: cellulose, vitamin B, Vitamin B5, vitamin B6, thyroxine, adrenaline, potassium ion, magnesium ion, sodium ion. 015 - 10: 1, more preferably 0. 02 - 0. 7, the weight ratio of the weight of the pentose saccharide and the sugar absorbing agent, such as the microcrystalline cellulose is 0. 001 - 1000: 1 , preferably 0. 015 - 10: 1, more preferably 0. 02 - 0. : 1

The composition provided by the present invention can be obtained by mixing a fiber pentose with one or more substances selected from the group consisting of cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, adrenaline, potassium ion, magnesium. Ions, sodium ions. Mixing, including conventional mixing, co-crystallization, co-drying, said vitamins, thyroxine, adrenaline, potassium ions, magnesium ions, sodium ions, etc., in the composition is the highest safe level they allow.

The substance for enhancing the absorption of the five-carbon sugar in the present invention includes cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, adrenaline, potassium ion, magnesium ion, sodium ion, and the like. The cellulose is present in the composition in an amount between 1% and 99%.

In another preferred embodiment of the invention, a method of producing a composition of the invention is provided, comprising the steps of:

(a) the pulverized cellulosic biomass is dewaxed using near boiling hot water;

(b) Dewaxed cellulosic biomass is used for the hydrolysis to prepare a fiber pentose product, and the hydrolysis is generally carried out in pure water. The hydrolysis catalyst is generally a hemicellulose hydrolase, an organic acid, a mineral acid, and a biomimetic catalyst;

(c) solid-liquid separation to obtain a solution containing cellulose pentose, separating or inactivating the catalyst;

(d) removing the contained six carbon sugars by fermentation;

(e) obtaining a liquid product after solid-liquid separation;

(f) Mix with the required amount of sugar absorption enhancer to obtain the desired product. Use

The fiber pentose or the fiber pentose-containing composition provided by the present invention can be used for the preparation of a product for preventing and/or treating the acidification of a body of a diabetic patient, which can be a general food, a health food, or a medicine.

The fiber pentose or the fiber pentose-containing composition provided by the present invention can also be used for the preparation of a drug for promoting the absorption of pentose sugar, and particularly for the preparation of a drug for promoting the absorption of arabinose.

The drug can be obtained by mixing the fiber pentose or the fiber pentose-containing composition provided by the present invention with a pharmaceutically acceptable carrier.

The term "pharmaceutically acceptable carrier" as used herein refers to a carrier for the administration of a therapeutic agent, Various excipients and diluents are included. The term refers to pharmaceutical carriers which are not themselves essential active ingredients and which are not excessively toxic after administration. Suitable carriers are well known to those of ordinary skill in the art. A full discussion of pharmaceutically acceptable excipients can be found in Remi ngton's Pharmac eut i cal Sc i enc es (Mack Pub. Co., NJ 1991). Pharmaceutically acceptable carriers in the compositions may include liquids such as water, saline, glycerol and ethanol. In addition, auxiliary substances such as disintegrants, wetting agents, emulsifiers, pH buffering substances and the like may also be present in these carriers.

The medicament may be prepared into various dosage forms, such as a solid preparation, or a liquid preparation, depending on the administration route. These dosage forms are administered in one of the following ways: oral, spray inhalation, rectal administration, nasal administration, buccal administration, topical administration, parenteral administration, such as subcutaneous, intravenous, intramuscular, intraperitoneal, intrathecal, intraventricular, intrasternal And intracranial injection or input, or with an explant reservoir. The present invention provides a product for preventing acidification of a body of a diabetic patient, and more particularly to provide a natural five-carbon sugar monomer or a composition therebetween, and at the same time, to provide a body capable of promoting five-carbon sugar by the body. The method of absorption allows diabetic patients to still use the energy of sugar source, help prevent acidification of the body of diabetic patients, reduce the level of oxidative stress, and prevent chronic complications of diabetes. Natural five-carbon sugars are found in virtually all cellulose-containing biomass, including cellulosic biomass, including their seeds, fruits, stems, leaves, roots, and the like. These polymers of pentose sugars are more easily hydrolyzed into monosaccharides during the production of food. Their metabolism in the body does not require the participation of insulin, which can stimulate the health and development of other functions of the body, while at the same time reducing the islets of the islets. Work stress is very important to maintain the health of the human body. However, due to so-called technological advances, the proportion of finishing in human food is increasing, and the intake of these natural five-carbon sugars is greatly reduced. Although humans are trying to supplement, such as the preparation of fruit vinegar and other products in order to increase the body's natural five-carbon sugar intake, but due to the influence of human diet, the population of such products is very small. Therefore, the natural five-carbon sugar composition provided in the present invention is also of great significance to the average consumer. Since natural arabinose has been shown to be the worst-absorbing five-carbon sugar in the research results to date, the present invention is exemplified by natural arabinose. It is to be understood that natural arabinose is used as an example to better illustrate the invention and is not intended to limit the scope of the invention. The above-mentioned features mentioned in the present invention, or the features mentioned in the embodiments, may be arbitrarily combined. All of the features disclosed in the present specification can be used in combination with any of the compositions, and the various features disclosed in the specification can be substituted for any alternative feature that provides the same, equal or similar purpose. Therefore, unless otherwise stated, the disclosed features are only a general example of equivalent or similar features. The main advantages of the invention are:

1. The product provided by the invention effectively reduces the acidification of the body of a diabetic patient;

2. The product provided by the invention can restore the normal living habits of diabetic patients;

3. The composition provided by the invention can effectively promote the absorption of five carbon sugars in the body;

4. The invention achieves low cost of obtaining natural five-carbon sugar. The invention is further illustrated below in conjunction with specific embodiments. It is to be understood that the examples are not intended to limit the scope of the invention. The experimental methods in which the specific conditions are not indicated in the following examples are usually carried out according to conventional conditions or according to the conditions recommended by the manufacturer. All percentages, ratios, ratios, or parts are by weight unless otherwise indicated.

The unit of weight percent by volume in the present invention is well known to those skilled in the art and, for example, refers to the weight of the solute in a 100 ml solution.

Unless otherwise defined, all professional and scientific terms used herein have the same meaning as those skilled in the art. In addition, any methods and materials similar or equivalent to those described can be used in the methods of the invention. The preferred embodiments and materials described herein are for illustrative purposes only. Example 1A

Preparation of fiber pentose I

1000 g (dry weight) The smashed wheat straw (half cellulose content is 21%) is contacted with hot water close to the boiling point (for example, washed with hot water). After 10 minutes, try to press it to moisture and transfer it to 10 liters. In a pressure reactor, then 50 mM of a biomimetic catalyst [CpFeCO (maleic acid)] BF4 (patent application PCT/CN/2007/071 1 14 ) was added, and the mixture was heated to near 100 °C with stirring and maintained at this temperature. After 60 minutes, after cooling to room temperature, solid-liquid separation gave a liquid product.

The 5% of the bean cake powder is added after the 5% of the bean cake powder is added to the 5% of the bean cake powder. After sterilization for 30 minutes at 121 oC, add 1% high-efficiency Anqi dry yeast and ferment for 24 hours at 34 °C. Sampling and testing, six carbon sugars are basically not detected, and the yeast is inactivated by heating, and the solid-liquid separation is carried out by filtration to obtain a liquid product.

Liquid products are ion exchanged (732-cation exchange resin column, 721 - anion exchange resin column, and mixed bed ion exchange column), concentrated to sugar content (total sugar content, including all five carbon sugars, using Abbe refractometer, Accurate quantification by liquid chromatography) Approximately 50% aqueous solution, wherein the arabinose content is about 12%, and the total liquid product obtained is 260 ml. The HPLC chromatogram of the product only detects arabinose and xylose, and the six carbon sugar does not. Observed. The preparation was repeated several times to obtain sufficient solid product preparation.

Liquid Chromatography HPLC is a classical monosaccharide analysis method using LC-NH2 of SUPELC0S IL From the column (250X4. 6mm), the mobile phase is acetonitrile: water = 3: 1, the flow rate is 1 ml / min, the refractive index detector, the retention time of ribose is about 5.8 minutes, and the retention time of xylose is 6. 6分钟左右。 The retention time of the arabinose is about 7.3 minutes, the retention time of the fructose is about 8.2 minutes, the retention time of the glucose is about 7.9 minutes, and the retention time of the galactose is about 10. 2 minutes.

If it is necessary to prepare a solid product, it can be obtained by lyophilization (135 g of a solid product obtained by lyophilization, water content of 2%) or by spray drying; if it is necessary to prepare a liquid product, the package can be sealed under inert gas protection. Example 1B

1000 g (dry weight) of crushed corn bran, mixed with pure water to prepare a 5% mixture, transferred to a 10 liter pressure reactor, and then added 50 mM biomimetic catalyst [CpFeCO (maleic acid)] BF4, The mixture was heated to near 100 ° C with stirring and maintained at this temperature for about 60 minutes. After cooling to room temperature, solid-liquid separation gave a liquid product.

The 5% of the bean cake powder is added after the 5% of the bean cake powder is added to the 5% of the bean cake powder. After sterilization for 30 minutes at 121 oC, 1% high-efficiency Anqi dry yeast was added and fermented at 34 °C for 24 hours. Sampling and detection, HPLC analysis method is basically undetectable. After the yeast is inactivated by heating, the solid-liquid separation is carried out by filtration to obtain a liquid product.

The liquid product is ion exchanged (732-cation exchange resin column, 721 - anion exchange resin column, and mixed bed ion exchange column), and concentrated to a sugar content (total sugar content, using an Abbe refractometer) of about 50%, wherein The arabinose content is about 53%, and the total liquid product (ie, fiber pentose) is 380.

Example 2

The freeze-dried fiber pentose solid product (ie, fiber pentose I) and microcrystalline cellulose are mixed to make the content of microcrystalline cellulose 10%, 20%, 30%, 40%, and they are used to feed half. The old monkey, the monkey's living environment is manually controlled 12 hours day / 12 hours night, room temperature is 24 ° C, after feeding normal food for 7 days, the food is incorporated into the microcrystalline cellulose, and no microcrystalline fiber is added. The fiber pentose sugar, the amount of arabinose in the food is 20, 40, 60, 80 g / kg of feed, the urine of the collected animal is analyzed for the arabinose content, the results are as follows: Feed containing microcrystalline cellulose containing microcrystalline cellulose containing microcrystalline cellulose containing microcrystalline cellulose containing microcrystalline cellulose containing labo sugar amount 0 amount 10% amount 20% amount 30% amount 40%

20 10. 1 6. 3 4. 6 3. 3 1. 1

40 12. 3 7. 5 5. 2 3. 8 1. 7

60 14. 7 8. 2 5. 9 4. 3 1. 9

80 15. 5 8. 9 6. 4 4. 9 2. 1

(The percentage is the percentage of arabinose in the food that is excreted. The amount of urine in 24 hours.) The results show that the unabsorbed arabinose is excreted in the urine and its discharge increases with the amount of microcrystalline cellulose. And reduce. Example 3

By the same experimental scheme as in Example 2, it was shown that when the amount of vitamin B1 was 200 mg/kg of fiber pentose without microcrystalline cellulose, the absorption of the fiber pentose sugar was increased by 16%; the vitamin B5 was 200 mg/ When gram of fiber pentose is used, the absorption of pentose can be increased by 21%; when vitamin B6 is 200 mg/kg of pentose, the absorption of pentose can be increased by 11%; when thyroxine is 30 mg/kg of pentose , the absorption of pentose can increase by 9%; when the adrenaline is 20 mg / kg of pentose, the absorption of pentose can increase by 11%; when the potassium ion is 200 mg / kg of pentose, the pentose Absorption can be increased by 27%; when magnesium ion is 200 mg/kg of fiber pentose, the absorption of pentose can be increased by 16%; when sodium ion is 200 mg/kg of pentose, the absorption of pentose can be increased by 6%. Example 4A

The sputum-type diabetic rats prepared with streptozotocin were 9 weeks old, the first group was the control group, 6 were fed with pure protein food, and metformin was used to control blood glucose; the second group of protein feed was used in Example 2 Fibrous pentose of microcrystalline cellulose, the arabinose content in the feed is 80 g/kg, and the content of microcrystalline cellulose in the fiber pentose product is 40%. After three months of feeding, the blood is taken from the tail vein. After adding the anti-coagulant, the supernatant is obtained by centrifugation, and the pH of the blood is measured by a precision pH meter. The results are as follows:

The results show that the product disclosed in the present invention can effectively prevent acidification of the diabetic body. Example 4B

Under the protection of nitrogen, the lyophilized fiber pentose solid product (ie, fiber pentose I) 800 g was added to 3 liters of 95% edible ethanol, then heated to reflux, and slowly added to about 100 ml. After pure water, reflux for about 10 minutes, slowly cool to 20 ° C, add a small amount of xylose seed crystals, and then store at this temperature for 24 hours, occasionally gently shaking. After 24 hours, the mixture was cooled to 0 ° C, and placed for 48 hours, filtered, and dried under vacuum to obtain 500 g of pure xylose with a purity of 98.6%.

The sputum-type diabetic rats prepared with streptozotocin were 9 weeks old, the first group was the control group, 6 were fed with pure protein food, and metformin was used to control blood glucose; the second group of protein feed was prepared using the pure wood prepared above. Sugar, the xylose content in the feed is 120 g / kg, does not contain additional microcrystalline cellulose, after three months of feeding, determination (take blood from the tail vein, add anti-coagulant, centrifuge to obtain clear liquid, precision pH meter determination) The pH of their blood, the results are as follows:

The results show that pure five-carbon sugar also has the function of preventing acidification of the body of the diabetic body. All documents mentioned in the present application are hereby incorporated by reference in their entirety in their entirety in their entireties in the the the the the the the the In addition, it is to be understood that various modifications and changes may be made to the present invention, and the equivalents of the scope of the present invention.

Claims

Rights request

A use of a pentose pentose or a pentose-containing composition, which is for use in the preparation of a food or a medicament for preventing and/or treating acidification of a body of a diabetic patient.

The use according to claim 1, wherein the fiber pentose-containing composition is composed of a fiber pentose sugar and a substance which promotes absorption of the fiber pentose sugar in the body.

3. Use according to claim 2, characterized in that, based on 100 parts by weight of the composition, it contains:

1 to 100 parts by weight of pentose sugar; and

0 - 99 parts by weight of a sugar absorption enhancer; the sugar absorption enhancer is selected from one or more of the following: cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, adrenaline, potassium ion, Magnesium ions, sodium ions.

015—10: 1,更更优选。 0. 015—10: 1, more The best place is 0. 02 - 0. 7: 1.

5. Use according to claim 3, characterized in that the cellulose comprises all cellulose, preferably microcrystalline cellulose, most preferably nano-sized cellulose.

The use according to any one of claims 1 to 4, wherein the fiber pentose sugar is selected from one or more of the following: xylose, ribose, arabinose ( Arab inose), lyxose, ribulose, xylulose.

The use according to claim 6, wherein the fiber pentose contains 1 to 90% by weight of arabinose, based on the total weight of the fiber pentose; preferably, the fiber pentose contains arabinose 5 25wt%.

The use according to any one of claims 1 to 4, wherein the fiber pentose is obtained by the following steps:

(b) After mixing the liquid mixture I and the alcohol yeast at 20-60 ° C for 6 to 48 hours, the solid matter is filtered off and concentrated to obtain a fiber pentose.

The use according to claim 8, wherein the fiber pentose is obtained by the following steps:

(1) mixing the cellulose-containing biomass, water and catalyst at 30-18 CTC for 3 to 600 minutes, and hydrolyzing to obtain a liquid mixture I; the catalyst is selected from the group consisting of: hemicellulose hydrolase, organic acid, inorganic acid, or a biomimetic catalyst; the organic acid is selected from the group consisting of: an organic acid containing less than 20 carbons; the inorganic acid is selected from the group consisting of: sulfuric acid, hydrochloric acid, phosphoric acid; and the biomimetic catalyst is selected from the group consisting of [CpFeCO (Malay) Acid)] BF4, or iron diammonium diacetate complex;

(3) The liquid mixture II is ion-exchanged to obtain a fiber pentose.

The use according to claim 1, wherein the food product comprises a solid food and a liquid food.

13. A method of preparing pure xylose from cellulosic biomass, characterized in that the method comprises the steps of: recrystallizing the fiber pentose sugar through a simple ethanol/water mixed solvent;

The fiber pentose is obtained by the following steps:

(3) The liquid mixture II is ion-exchanged to obtain a fiber pentose.