CN101507728B - Composite for preventing and treating body acidification of diabetic - Google Patents
Composite for preventing and treating body acidification of diabetic Download PDFInfo
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- CN101507728B CN101507728B CN200910127799.XA CN200910127799A CN101507728B CN 101507728 B CN101507728 B CN 101507728B CN 200910127799 A CN200910127799 A CN 200910127799A CN 101507728 B CN101507728 B CN 101507728B
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Abstract
The invention discloses a monomer or a composition for preventing and treating organism acidification of diabetics, and a preparation method and application thereof. Calculated as 100 weight portions of composition, the composition contains 1 to 100 weight portions of fiber pentose and 0 to 99 weight portions of sugar absorption enhancer, wherein the sugar absorption enhancer is selected from one or more of the following substances: cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, adrenalin, potassium ions, magnesium ions, and sodium ions.
Description
Technical field
The present invention relates to cellulose biomass field, relate in particular to a kind of compositions of preventing and treating body acidification of diabetic.
Background technology
Diabetes be body cannot excreting insulin or the quantity of excreting insulin or/and the mass deficiency of insulin and cause effectively a kind of disease of katabolism glucose of health.Current, diabetics generally adopts many edible proteins etc. to provide necessary energy supply for health.Because the metabolism of the materials such as protein is not the optimum energy supply mode of health, a large amount of this metabolism cause the not thorough of metabolism, and due in most protein molecule, contain a lot of hetero atoms, as nitrogen, sulfur and phosphorus, etc., the result of long-term accumulated is exactly the acidify of health.
Body acidification is the ubiquitous phenomenon of Disease and old people, and healthy person and teen-age body are all subalkaline.In sour environment, many high activity particles are as easier in the generation of free radical, the oxidative stress level of body is raise, and oxidative stress horizontal abnormality is various cell injury and dead basic reason in body, the oxidative stress of improper level is considered to cause diabetes and chronic complicating diseases of diabetes, the major reason of the diseases such as pulmonary fibrosis, epilepsy, hypertension, atherosclerosis and corresponding cardiovascular disease, cancer, nephropathy and parkinson disease.Find according to clinical evaluating result, a lot of patients, as cancer patient, are acidic physique substantially, maintain body in alkalescence state, are one of important means reducing oxidative stress level.
The Acidity of Aikalinity of food, not determines with mouthfeel, but digested and assimilated in vivo by food, the result that produces after metabolism determines.If the anion ratio such as the phosphate radical producing after food metabolism, sulfate radical, nitrate anion, chloride ion are more, will cause body acidification, be acid food; If the sodium ion producing after food metabolism, potassium ion, magnesium ion, calcium ion plasma are more, be just easy to cause body alkalization, be basic food.Although mouthfeel acidity such as citric acids, human body metabolism is translated into water and carbon dioxide, and it belongs to neutral food; Protein does not have soda acid taste, but human body will can produce the aniones such as sulfate radical, nitrate anion, chloride ion after its metabolism, and they belong to acid food; And the final metabolite of glucide is all water and carbon dioxide.As can be seen here, when after diabetics morbidity, the main body of its food is from carbohydrate transfers protein to, and acidify very easily occurs its health, causes oxidative stress level further to raise.
The metabolism of natural pentose does not need the participation of insulin, and their sugariness is similar to glucose, so natural pentose is ideal glucose substitutes saccharide, the dietary habit that the material of sugared source energy is provided and recovers diabetics for diabetics be as eaten sugar.But, because two reasons have limited their uses in life.The one, pentose absorbed degree in health is on the low side, and the 2nd, their production cost is too high and cause most people not consume.
Therefore, this area is in the urgent need to providing a kind of material that contains natural pentose, and degree of absorption is high in vivo for it, can effectively prevent and treat body acidification of diabetic, and production cost is low.
Summary of the invention
The purposes of the compositions that the present invention aims to provide a kind of fiber pentose or contains fiber pentose.
Another object of the present invention is to provide a kind of method of preparing pure xylose from cellulose biomass.
In a first aspect of the present invention, the purposes of the compositions that a kind of fiber pentose is provided or has contained fiber pentose, for the preparation of the food or the medicine that prevent and/or treat body acidification of diabetic.
In another preference, the material that the described compositions that contains fiber pentose is absorbed in vivo by fiber pentose and promotion fiber pentose forms.
In another preference, in 100 weight portion compositionss, it contains:
1-100 weight portion fiber pentose; With
0-99 weight portion sugar absorption enhancer; Described sugared absorption enhancer is selected from one or more following materials: cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion.
In another preference, in 100 weight portion compositionss, it contains: 1-100 weight portion fiber pentose; With 0.001-99 weight portion sugar absorption enhancer.
In another preference, the weight ratio of described fiber pentose and sugared absorption enhancer is 0.001-1000: 1, be preferably 0.015-10: 1, be more preferably 0.02-0.7: 1.
In another preference, the material that described promotion fiber pentose absorbs in vivo comprises all can promote the material that fiber pentose absorbs in vivo, both can be macromolecular substances, also can be small-molecule substance, ion even, include but not limited to cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion, etc.
In another preference, described cellulose comprises all celluloses, preferably microcrystalline cellulose, most preferably nano level cellulose.
In another preference, described fiber pentose is selected from following one or more: xylose (xylose), ribose (ribose), arabinose (arabinose), lyxose (lyxose), ribulose (ribulose), xylulose (xylulose).
In another preference, described fiber pentose obtains by following steps:
(a) will contain cellulosic biomass and water and mix, obtain liquid mixture I 20-180 DEG C of hydrolysis; With
(b) liquid mixture I and distillery yeast are mixed after 6-48 hour to elimination solids, the concentrated fiber pentose that obtains at 20-60 DEG C.
In another preference, described fiber pentose obtains by following steps:
(1) will contain cellulosic biomass, water and catalyst and mix 3-600 minute at 30-180 DEG C, hydrolysis obtains liquid mixture I; Described catalyst is selected from: hydrolysis of hemicellulose enzyme, organic acid, mineral acid or bionic catalyst; Described organic acid is selected from: containing 20 organic acid below carbon; Described mineral acid is selected from: sulphuric acid, hydrochloric acid, phosphoric acid; Described bionic catalyst is selected from [CpFeCO (maleic acid)] BF4 or second diamino oxalic acid iron complex;
(2) liquid mixture I and distillery yeast are mixed to 6-48 hour at 20-60 DEG C, obtain liquid mixture II; With
(3) liquid mixture II, through ion exchange, obtains fiber pentose.
In another preference, described fiber pentose, in fiber pentose gross weight, contains arabinose 1-90wt%; Preferably in described fiber pentose, contain arabinose 5-25wt%.
In another preference, described fiber pentose obtains by following steps:
(a) will contain cellulosic biomass and water and mix, obtain liquid mixture I 90-180 DEG C of hydrolysis; With
(b) liquid mixture I and distillery yeast are mixed to 12-48 hour at 20-40 DEG C, obtain fiber pentose.
In another preference, described fiber pentose obtains by following steps:
(1) will contain cellulosic biomass, water and catalyst and mix 30-120 minute at 90-180 DEG C, hydrolysis obtains liquid mixture I; Described catalyst is selected from: hydrolysis of hemicellulose enzyme, organic acid, mineral acid or bionic catalyst; Described organic acid is selected from: containing 20 organic acid below carbon; Described mineral acid is selected from: sulphuric acid, hydrochloric acid, phosphoric acid; Described bionic catalyst is selected from: [CpFeCO (maleic acid)] BF4 or second diamino oxalic acid iron complex;
(2) liquid mixture I and distillery yeast are mixed to 12-48 hour at 20-40 DEG C, obtain liquid mixture II; With
(3) liquid mixture II, through ion exchange, obtains fiber pentose.
In another preference, described food comprises solid food and liquid food.
In a second aspect of the present invention, a kind of method of preparing pure xylose from cellulose biomass is provided, described method comprises step: by the simple ethanol/water mixed solvent recrystallization of fiber pentose process;
Described fiber pentose obtains by following steps:
(1) will contain cellulosic biomass, water and catalyst and mix 30-120 minute at 90-180 DEG C, hydrolysis obtains liquid mixture I; Described catalyst is selected from: hydrolysis of hemicellulose enzyme, organic acid, mineral acid or bionic catalyst; Described organic acid is selected from: containing 20 organic acid below carbon; Described mineral acid is selected from: sulphuric acid, hydrochloric acid, phosphoric acid; Described bionic catalyst is selected from: [CpFeCO (maleic acid)] BF4 or second diamino oxalic acid iron complex;
(2) liquid mixture I and distillery yeast are mixed to 12-48 hour at 20-40 DEG C, obtain liquid mixture II; With
(3) liquid mixture II, through ion exchange, obtains fiber pentose.
Accordingly, the invention provides a kind of material that contains natural pentose, degree of absorption is high in vivo for it, can effectively prevent and treat body acidification of diabetic, and production cost is low.
Detailed description of the invention
Inventor is through extensive and deep research, find by containing cellulosic biorefinery, can obtain the product of natural pentose: fiber pentose, after it mixes with some materials, can greatly improve the effective absorption in vivo of natural pentose, thereby the product of sugared source energy can be provided for diabetics, prevent that diabetics from causing the acidify of body for energy matter because of the long-term main protein that uses, and then there is the oxidative stress of reduction, prophylactic function.
Definition
As used herein, " biomass of cellulose " and " containing cellulosic biomass " can exchange use, all refer to all cellulosic materials that contain, its scope comprises the cellulose biomass in traditional definition, the seed, fruit, stem, leaf and the root that comprise plant, even comprise some microorganisms.Described plant comprises that the characteristic of all nontoxic plants, product is not subject to the impact of plant origin.All contain cellulosic biomass and all can use, preferably the content of hemicellulose is counted material more than 5wt% with cellulose biomass gross weight, and most preferably the content of hemicellulose is counted material more than 10wt% with cellulose biomass gross weight.
As used herein, " cellulose biomass " refers to the biomass taking cellulose, hemicellulose and lignin as main component, generally do not comprise the fruit of plant.
Microcrystalline Cellulose is a kind of pure native cellulose depolymerization product, is fine powder powder, white, and odorlessness, without taste; By forming by free-pouring crystalline powder (non-fibrous micropartical).Water insoluble, to dilute acid and most organic solvents, be slightly soluble in 20% aqueous slkali.Dry powder sheeting be can be directly used in, drug excipient, flow aid, implant, disintegrating agent, antiplastering aid, adsorbent, capsule diluent etc. are widely used as.Nano micro crystal cellulose refers to that cross-section lengths is 10-20 nanometer in the present invention, and fibre length is the cellulosic material that is less than 100 nanometers.
As used herein, fiber pentose is selected from all natural pentoses, as the xylose of five-carbon ring aldehydo sugar (xylose), ribose (ribose), arabinose (arabinose), lyxose (lyxose), the ribulose (ribulose) of five-carbon ketone sugar, xylulose (xylulose), etc.Fiber pentose can be both single pure pentose, can be also their mixture, and the content of each sugar in mixture is not limit.In the present invention, " fiber pentose ", " natural pentose compositions " or " natural pentose " can exchange use, all refer to monosaccharide or their simple derivant or the polymer containing five carbon atoms of the natural generation of all occurring in natures.
As used herein, " bionic catalyst " refers to the catalyst of the synthetic that at least contains a catalysis region (catalytic domain) and a cellulose binding domain (cellulose binding domain), as [CpFeCO (maleic acid)] BF4, second diamino oxalic acid iron complex, etc.In patent application PCT/CN/2007/071114, bionic catalyst is had to detailed description, the bionic catalyst that wherein disclosed all can be for product preparation herein.
On the other hand, fiber pentose of the present invention can obtain by following step:
(a) will contain cellulosic biomass and water and mix, obtain liquid mixture I 20-180 DEG C of hydrolysis; With
(b) liquid mixture I and distillery yeast are mixed to 6-48 hour at 20-60 DEG C, elimination solids, the concentrated fiber pentose that obtains.
In a preferred embodiment of the present invention, obtain fiber pentose by following steps:
The first step, is used water (80-98 DEG C is the preferably 90-95 DEG C) dewaxing of closely boiling by the cellulosic biomass that contain of pulverizing;
Second step, mixes 3-600 minute by contain cellulosic biomass and catalyst after dewaxing at 30-140 DEG C, and hydrolysis, solid-liquid separation, obtain liquid mixture I; Described catalyst is selected from: hydrolysis of hemicellulose enzyme, organic acid, mineral acid or bionic catalyst; Described organic acid is selected from: containing 20 organic acid below carbon; Described mineral acid is selected from: sulphuric acid, hydrochloric acid, phosphoric acid; Described bionic catalyst is selected from: patent application PCT/CN/2007/071114 discloses;
The 3rd step, separates or deactivation catalyst; Described separation comprises passes through macroporous adsorbent resin by liquid mixture I; Described deactivation comprise heating, neutralization, etc.;
The 4th step, mixes 6-48 hour by liquid mixture I and distillery yeast at 20-60 DEG C, and elimination solid, obtains product liquid II;
The 5th step, through ion exchange, obtains fiber pentaglucose liquid by product liquid II.
Ion exchange in the 5th step comprises cation exchange and anion exchange, sometimes, in order to improve the quality of product, also adds a mixed column that simultaneously contains cationic resin and resin anion (R.A.).Eluting is all generally to use pure water, and the regeneration of exchanger resin is all generally sodium hydrate aqueous solution and dilute hydrochloric acid.
In the compositions of control body acidification of diabetic provided by the invention, contain fiber pentose and pentose absorption enhancer; Described sugared absorption enhancer is selected from one or more following materials: cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion.Fiber pentose wherein and sugared absorption enhancer as the weight ratio of microcrystalline Cellulose be 0.001-1000: 1, be preferably 0.015-10: 1, be more preferably 0.02-0.7: 1.
Compositions provided by the invention can be by by fiber pentose be selected from one or more following materials and be mixed to get: cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion.Mix and comprise conventional mixing, cocrystallization, be dried altogether, etc.
The content in compositions such as said vitamin, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion is the highest safe level that they allow.
In the present invention for strengthen material that pentose absorbs comprise cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion, etc.The content of said cellulose in compositions is between 1%-99%.
In another preferred embodiment of the present invention, a kind of method of producing the present composition is provided, comprise the steps:
(a) comminuted fibres cellulosic biomass uses the hot water dewaxing of closely boiling;
(b) cellulose biomass after dewaxing is prepared fiber pentose product for hydrolysis, and hydrolysis is generally carried out in pure water, and hydrolyst is generally hydrolysis of hemicellulose enzyme, organic acid, mineral acid and bionic catalyst;
(c) solid-liquid separation obtains the solution of fibre-bearing pentose, separates or deactivation catalyst;
(d) fermentation method is removed contained hexose;
(e) after solid-liquid separation, obtain fluid product;
(f) and the sugared absorption enhancer mix homogeneously of requirement obtain need product.
Purposes
Fiber pentose provided by the invention or the compositions that contains fiber pentose can be used for preparing the product that prevents and/or treats body acidification of diabetic, can be both normal food, health food or medicine.
Fiber pentose provided by the invention or the compositions that contains fiber pentose can also, for the preparation of the medicine that promotes that fiber pentose absorbs, especially can be used for the medicine that preparation promotes that arabinose absorbs.
Described medicine can be by being mixed to get fiber pentose provided by the invention or the compositions that contains fiber pentose and pharmaceutically acceptable carrier.
As used herein, term " pharmaceutically acceptable carrier " refers to be used for the treatment of the carrier of agent administration, comprises various excipient and diluent.This term refers to some medicament carriers like this: they itself are not necessary active component, and after using, there is no undue toxicity.Suitable carrier is well known to those of ordinary skill in the art.In Remington ' s PharmaceuticalSciences (Mack Pub.Co., N.J.1991), can find discussing fully about pharmaceutically acceptable excipient.On combination of Chinese medicine is learned, acceptable carrier can comprise liquid, as water, saline, glycerol and ethanol.In addition, in these carriers, also may there is complementary material, as disintegrating agent, wetting agent, emulsifying agent, pH buffer substance etc.
Described medicine can be prepared into various dosage forms according to different way of administration, as solid preparation, or liquid preparation.These dosage forms are used in one of mode below: oral, spraying suction, rectal application, nasal cavity applied medicine, buccal medication, local application, non-enterally administer, as in subcutaneous, vein, muscle, intraperitoneal, sheath, in ventricle, in breastbone and intracranial injection or input, or by the medication of a kind of outer planting reservoir.
The invention provides a kind of product that prevents the acidify of diabetics health, concretely, be to provide a kind of monomer of natural pentose or the compositions between them, simultaneously, a kind of method that can promote that pentose is absorbed by health is also provided, makes diabetics still can use sugared source energy, assist to prevent the body acidification of diabetics, reduce oxidative stress level, prevent diabetes chronic complicating diseases.
Natural pentose is present in all containing in cellulosic biomass substantially, comprises in cellulose biomass, comprise they seed, fruit, stem, leaf, root, etc.The polymer of this fibrid pentose is monosaccharide compared with facile hydrolysis in the manufacturing process of food, their metabolism in vivo do not need the participation of insulin, the sound of other function of health and development can be stimulated, the operating pressure of islets of langerhans can be alleviated again simultaneously, extremely important to maintaining the health of human body.But due to so-called technological progress, in human food, accurately machined proportion is increasing, the absorption of these natural pentoses significantly reduces.Although the mankind supplement managing again, as prepare the products such as fruits vinegar to increasing the absorption of the natural pentose of health,, due to the impact of human diet custom, use the population of this series products little.So, this natural pentose compositions is provided in the present invention, concerning general consumer, also possess important meaning.
Be all to absorb the poorest pentose because natural arabinose shows in achievement in research up to now, the present invention illustrates as an example of natural arabinose example.Should be understood that as an example of natural arabinose example it is only for better explanation the present invention, instead of limit the scope of the invention.
The above-mentioned feature that the present invention mentions, or the feature that embodiment mentions can combination in any.All features that this case description discloses can with any composition forms use, each feature disclosing in description, can anyly provide the alternative characteristics of identical, impartial or similar object to replace.Therefore apart from special instruction, the feature disclosing is only the general example of equalization or similar features.
Major advantage of the present invention is:
1, product provided by the invention effectively reduces the acidify of diabetics body;
2, product provided by the invention can recover custom orthobiosis of diabetics;
3, compositions provided by the invention can effectively promote pentose absorption in vivo;
4, the present invention obtains the with low cost of natural pentose.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.The experimental technique of unreceipted actual conditions in the following example, the condition of conventionally advising according to normal condition or according to manufacturer.Unless otherwise indicated, otherwise all percent, ratio, ratio or umber by weight.
Unit in percent weight in volume in the present invention is well-known to those skilled in the art, for example, refer to the weight of solute in the solution of 100 milliliters.
Unless otherwise defined, the same meaning that all specialties that use in literary composition and scientific words and one skilled in the art are familiar.In addition, any method similar or impartial to described content and material all can be applicable in the inventive method.The use that better implementation method described in literary composition and material only present a demonstration.
Embodiment 1A prepares fiber pentose I
1000 grams of (dry weight) chippy wheat straws (hemicellulose level is 21%) contacted (for example using hot water injection) after 10 minutes with the hot water that approaches boiling point, after being pressed water purification to divide as far as possible, transfer in the pressure reaction still of 10 liters, then add bionic catalyst [CpFeCO (the maleic acid)] BF4 (patent application PCT/CN/2007/071114) of 50mM, under stirring, mixture is heated to nearly 100 DEG C and maintain this temperature approximately 60 minutes, after cool to room temperature, solid-liquid separation obtains fluid product.
Fluid product is by the XAD-4 macroporous adsorptive resins of 3cm × 60cm, flow velocity 2 ml/min, in the fiber pentose aqueous solution of gained, substantially can't detect catalyst, then adjust pH to add approximately 1.5% soybean cake powder after 5.5,121 DEG C of sterilizings add 1% efficient Angel brewer's dried yeast after 30 minutes, 34 DEG C of bottom fermentations 24 hours.Sampling detects, and hexose can't detect substantially, and heating, by after yeast deactivation, is filtered and carried out solid-liquid separation, obtains fluid product.
Fluid product is crossed ion exchange (732-cation exchange resin column, 721-anion-exchange resin column and mixed bed ion exchange column), be concentrated to sugar content (total sugar content, comprise all pentoses, use abbe's refractometer, liquid chromatograph accurate quantification) be about 50% aqueous solution, wherein the content of arabinose is about 12%, and the fluid product altogether obtaining is 260 milliliters, the HPLC chromatograph of this product only detects arabinose and xylose, and hexose does not observe.Repeatedly preparation, for subsequent use to obtain enough solid products.
Liquid chromatography HPLC is classical monosaccharide analytical method, use the LC-NH2 detached dowel (250X4.6mm) of SUPELCOSIL, mobile phase is acetonitrile: water=3: 1, flow velocity is 1 ml/min, RI-detector, the retention time of ribose about 5.8 minutes, the retention time of xylose about 6.6 minutes, the retention time of arabinose about 7.3 minutes, the retention time of fructose about 8.2 minutes, the retention time of glucose about 9.7 minutes, the retention time of galactose is about 10.2 minutes.
If need to prepare solid product, can adopt lyophilizing (lyophilization obtains 135 grams of solid products, and water content is 2%) or spray drying method to make; If need to prepare fluid product, pack at inert gas shielding lower seal.
Embodiment 1B
1000 grams of (dry weight) chippy corn brans, adding pure water is formulated as after 5% mixture, transfer in the pressure reaction still of 10 liters, then add bionic catalyst [CpFeCO (the maleic acid)] BF4 of 50mM, under stirring, mixture is heated to nearly 100 DEG C and maintain this temperature approximately 60 minutes, after cool to room temperature, solid-liquid separation obtains fluid product.
Fluid product is by the XAD-4 macroporous adsorptive resins of 3cm × 60cm, flow velocity 2 ml/min, in the fiber pentose aqueous solution of gained, substantially can't detect catalyst, then adjust pH to add approximately 1.5% soybean cake powder after 5.5,121 DEG C of sterilizings add 1% efficient Angel brewer's dried yeast after 30 minutes, 34 DEG C of bottom fermentations 24 hours.Sampling detects, and HPLC analytical method hexose can't detect substantially, and heating, by after yeast deactivation, is filtered and carried out solid-liquid separation, obtains fluid product.
Fluid product is crossed ion exchange (732-cation exchange resin column, 721-anion-exchange resin column and mixed bed ion exchange column), be concentrated to sugar content (total sugar content, use abbe's refractometer) be about 50%, wherein the content of arabinose is about 53%, and the fluid product (being fiber pentose) altogether obtaining is 380 milliliters.
Embodiment 2
Fiber pentose solid product (being fiber pentose I) prepared by lyophilization and microcrystalline Cellulose mix, the content that makes microcrystalline Cellulose is 10%, 20%, 30%, 40%, with they carry out half years old large monkey of feeding, the living environment of monkey is manually operated 12 hours daytime/12 hour nights, room temperature is 24 DEG C, the normal food of feeding is after 7 days, in food, be impregnated in and add microcrystalline Cellulose, do not add the fiber pentose of microcrystalline Cellulose, make the amount that contains arabinose in food be respectively 20, 40, 60, 80 gs/kg of feedstuffs, the urinalysis of collection animal arabinose content wherein, result is as follows:
| Feedstuff is containing arabinose amount | Microcrystalline cellulose cellulose content 0 | Microcrystalline cellulose cellulose content 10% | Microcrystalline cellulose cellulose content 20% | Microcrystalline cellulose cellulose content 30% | Microcrystalline cellulose cellulose content 40% |
| 20 | 10.1 | 6.3 | 4.6 | 3.3 | 1.1 |
| 40 | 12.3 | 7.5 | 5.2 | 3.8 | 1.7 |
| 60 | 14.7 | 8.2 | 5.9 | 4.3 | 1.9 |
| 80 | 15.5 | 8.9 | 6.4 | 4.9 | 2.1 |
(unit is percentage ratio, and the arabinose in food has and how much excreted.Voided volume in 24 hours) result shows, and unabsorbed arabinose is discharged by urine, and its output, lowers along with increasing of microcrystalline cellulose cellulose content.
Embodiment 3
By the experimental program identical with embodiment 2, show, during without microcrystalline Cellulose, when the amount of use vitamin B1 is 200 mg/kg fiber pentose, the absorption of fiber pentose can increase by 16%; When vitamin B5 is 200 mg/kg fiber pentose, the absorption of fiber pentose can increase by 21%; When vitamin B6 is 200 mg/kg fiber pentose, the absorption of fiber pentose can increase by 11%; When thyroxine is 30 mg/kg fiber pentose, the absorption of fiber pentose can increase by 9%; When epinephrine is 20 mg/kg fiber pentose, the absorption of fiber pentose can increase by 11%; When potassium ion is 200 mg/kg fiber pentose, the absorption of fiber pentose can increase by 27%; When magnesium ion is 200 mg/kg fiber pentose, the absorption of fiber pentose can increase by 16%; When sodium ion is 200 mg/kg fiber pentose, the absorption of fiber pentose can increase by 6%.
Embodiment 4A
Use streptozotocin 9 week of II-type diabetic mice of preparing large, first group is matched group, 6, and feeding pure protein food with metformin control blood glucose; In the second histone feedstuff, use the fiber pentose containing microcrystalline Cellulose in embodiment 2, arabinose content in feedstuff is 80 gs/kg, the content of microcrystalline Cellulose in fiber pentose product is 40%, after feeding three months, measure (from tail venous blood sampling, add after anticoagulant, after centrifugal clear liquid, Accurate pH instrumentation is fixed) acid-base value of their blood, result is as follows:
| 1 | 2 | 3 | 4 | 5 | 6 | |
| First group | pH＝6.56 | pH＝6.63 | pH＝6.49 | pH＝6.52 | pH＝6.73 | pH＝6.76 |
| Second group | pH＝7.05 | pH＝7.04 | pH＝7.11 | pH＝7.05 | pH＝7.06 | pH＝7.02 |
Result shows, the product disclosing in the present invention can effectively prevent the acidify of diabetes body.
Embodiment 4B
Under nitrogen protection; 800 grams of fiber pentose solid products (being fiber pentose I) prepared by lyophilization; join in 95% the edible ethanol of 3 liters; then reflux, slowly adds the pure water that is about 100 milliliters, refluxes after approximately 10 minutes; slowly be cooled to 20 DEG C; add after a small amount of xylose crystal seed, this temperature lower seal is deposited 24 hours, rocks gently once in a while during this time.After 24 hours, be cooled to 0 DEG C of zero degree, put 48 hours, filter, dry under vacuum, obtain 500 grams of pure xyloses, purity is 98.6%.Use streptozotocin 9 week of II-type diabetic mice of preparing large, first group is matched group, 6, and feeding pure protein food with metformin control blood glucose; In the second histone feedstuff, use the pure xylose of above-mentioned preparation, Xylose Content in feedstuff is 120 gs/kg, not containing additional microcrystalline Cellulose, after feeding three months, measure (from tail venous blood sampling, add after anticoagulant, after centrifugal clear liquid, Accurate pH instrumentation is fixed) acid-base value of their blood, result is as follows:
| 1 | 2 | 3 | 4 | 5 | 6 | |
| First group | pH＝6.66 | pH＝6.53 | pH＝6.42 | pH＝6.53 | pH＝6.71 | pH＝6.69 |
| Second group | pH＝7.01 | pH＝6.94 | pH＝7.03 | pH＝6.97 | pH＝7.02 | pH＝6.92 |
Result demonstration, pure pentose also possesses the function that prevents glycosuria sick body body acidification.
All documents of mentioning in the present invention are all quoted as a reference in this application, are just quoted separately as a reference as each section of document.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read above-mentioned teachings of the present invention, these equivalent form of values fall within the application's appended claims limited range equally.
Claims (4)
1. a preparation method for fiber pentose, is characterized in that comprising the following steps:
The wheat straw of (1) 1000 gram of dry weight, pulverizing after 10 minutes, presses water purification to divide with hot water injection, transfers in the pressure reaction still of 10 liters, then adds bionic catalyst [CpFeCO (the maleic acid)] BF4 of 50mM, under stirring, mixture is heated to nearly 100
oc, and maintain this temperature approximately 60 minutes, after cool to room temperature, solid-liquid separation obtains fluid product I; The hemicellulose level of described wheat straw is 21%;
(2) aforesaid liquid product I is by the XAD-4 macroporous adsorptive resins of 3cm × 60cm, and flow velocity 2 ml/min, can't detect catalyst in the fiber pentose aqueous solution of gained substantially, then adjusts pH to add approximately 1.5% soybean cake powder, 121 after 5.5
oc sterilizing adds 1% efficient Angel brewer's dried yeast, 34 after 30 minutes
oc bottom fermentation 24 hours; Sampling detects, and hexose can't detect substantially; Heating, by after yeast deactivation, is filtered and is carried out solid-liquid separation, obtains fluid product II;
(3) fluid product II crosses ion exchange, is concentrated to sugar content and is about 260 milliliters of 50% aqueous solutions, obtains fiber pentose, and wherein the content of arabinose is about 12%; Described ion exchange is used 732-cation exchange resin column, 721-anion-exchange resin column and mixed bed ion exchange column.
2. a compositions that contains the fiber pentose that preparation method claimed in claim 1 obtains, is characterized in that, in 100 weight portion compositionss, it contains:
1-100 weight portion fiber pentose; With
0-99 weight portion sugar absorption enhancer; Described sugared absorption enhancer is selected from one or more following materials: cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion.
3. compositions as claimed in claim 2, is characterized in that, the weight ratio of described fiber pentose and sugared absorption enhancer is 0.02-0.7:1.
4. compositions as claimed in claim 2, is characterized in that, described cellulose preferably microcrystalline cellulose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910127799.XA CN101507728B (en) | 2009-03-24 | 2009-03-24 | Composite for preventing and treating body acidification of diabetic |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1366552A (en) * | 2000-02-01 | 2002-08-28 | 尤尼蒂卡株式会社 | Process for producing L-arabinose, L-arabinose-containing enzymatically processed products, diet foods, diabetic diet foods and fruit or vegetable juices and process for producing same |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1366552A (en) * | 2000-02-01 | 2002-08-28 | 尤尼蒂卡株式会社 | Process for producing L-arabinose, L-arabinose-containing enzymatically processed products, diet foods, diabetic diet foods and fruit or vegetable juices and process for producing same |
Non-Patent Citations (3)
| Title |
|---|
| 孙雪峰.体质分酸碱缺乏科学依据.《人生与伴侣：新养生》.2007,(第7期),16-17. * |
| 张彤 * |
| 蒋乃珺."酸碱体质"纯属谬论.《中国社区医师》.2008,第24卷(第19期),9. |
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