CN101507728A - Composite for preventing and treating body acidification of diabetic - Google Patents
Composite for preventing and treating body acidification of diabetic Download PDFInfo
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- CN101507728A CN101507728A CNA200910127799XA CN200910127799A CN101507728A CN 101507728 A CN101507728 A CN 101507728A CN A200910127799X A CNA200910127799X A CN A200910127799XA CN 200910127799 A CN200910127799 A CN 200910127799A CN 101507728 A CN101507728 A CN 101507728A
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Abstract
The invention discloses a monomer or a composition for preventing and treating organism acidification of diabetics, and a preparation method and application thereof. Calculated as 100 weight portions of composition, the composition contains 1 to 100 weight portions of fiber pentose and 0 to 99 weight portions of sugar absorption enhancer, wherein the sugar absorption enhancer is selected from one or more of the following substances: cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, adrenalin, potassium ions, magnesium ions, and sodium ions.
Description
Technical field
The present invention relates to the cellulose biomass field, relate in particular to a kind of compositions of preventing and treating body acidification of diabetic.
Background technology
Diabetes be body can't excreting insulin or the quantity of excreting insulin or/and the mass deficiency of insulin and cause effectively a kind of disease of katabolism glucose of health.Current, diabetics generally adopts polyphagia to wait for health with protein necessary energy supply is provided.Because the metabolism of materials such as protein is not the optimum energy method of supplying of health, a large amount of this metabolism cause metabolic not thorough, and since the most protein molecule in contain a lot of hetero atoms, as nitrogen, sulfur and phosphorus, or the like, the result of long-term accumulated is exactly the acidify of health.
Body acidification is disease patient and the ubiquitous phenomenon of old people, and healthy person and teen-age body all are subalkaline.In sour environment, the generation of many high activity particles such as free radical is easier, the oxidative stress level of body is raise, and the oxidative stress horizontal abnormality is various cell injury and a dead basic reason in the body, the oxidative stress of improper level is considered to cause diabetes and chronic complicating diseases of diabetes, the major reason of diseases such as pulmonary fibrosis, epilepsy, hypertension, atherosclerosis and corresponding cardiovascular disease, cancer, nephropathy change and parkinson disease.Finding that according to clinical evaluating result a lot of patients such as cancer patient are acidic physique basically, keep body and are in the alkalescence state, is one of important means that reduces the oxidative stress level.
The Acidity of Aikalinity of food is not to determine with mouthfeel, and the result that produced after the metabolism determines but digested and assimilated in vivo by food.If anion ratio such as the phosphate radical that is produced after the food metabolism, sulfate radical, nitrate anion, chloride ion are more, will cause body acidification, be acid food; If the sodium ion that is produced after the food metabolism, potassium ion, magnesium ion, calcium ion plasma are more, just be easy to cause the body alkalization, be basic food.Though mouthfeel acidity such as citric acids, the human body metabolism is translated into water and carbon dioxide, and it belongs to neutral food; Protein does not have the soda acid taste, but human body can produce aniones such as sulfate radical, nitrate anion, chloride ion after with its metabolism, and they belong to acid food; And the final metabolite of glucide all is water and carbon dioxide.This shows that after the diabetics morbidity, after the main body of its food transferred protein to from carbohydrate, acidify very easily took place its health, caused the oxidative stress level further to raise.
The metabolism of natural pentose does not need the participation of insulin, and their sugariness is similar to glucose, so natural pentose is very ideal glucose substitutes saccharide, the dietary habit that the material of sugared source energy is provided and recovers diabetics for diabetics be as eating sugar.But, owing to two reasons have limited their uses in life.The one, pentose absorbed degree in health is on the low side, and the 2nd, their production cost is too high and cause most people not consume.
Therefore, this area presses for provides a kind of material that contains natural pentose, and it is the degree of absorption height in vivo, can effectively prevent and treat body acidification of diabetic, and production cost is low.
Summary of the invention
The present invention aims to provide a kind of fiber pentose or contains the purposes of the compositions of fiber pentose.
Another object of the present invention provides a kind of method for preparing pure xylose from cellulose biomass.
In a first aspect of the present invention, a kind of fiber pentose is provided or has contained the purposes of the compositions of fiber pentose, be used to prepare the food or the medicine that prevent and/or treat body acidification of diabetic.
In another preference, the described compositions that contains the fiber pentose is made up of the material that fiber pentose and promotion fiber pentose absorb in vivo.
In another preference, in 100 weight portion compositionss, it contains:
1-100 weight portion fiber pentoses; With
0-99 weight portions sugar absorption enhancer; Described sugared absorption enhancer is selected from one or more following materials: cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion.
In another preference, in 100 weight portion compositionss, it contains: 1-100 weight portion fiber pentoses; With 0.001-99 weight portions sugar absorption enhancer.
In another preference, the weight ratio of described fiber pentose and sugared absorption enhancer is 0.001-1000:1, preferably is 0.015-10:1, more preferably is 0.02-0.7:1.
In another preference, the material that described promotion fiber pentose absorbs in vivo comprises all can promote the material that the fiber pentose absorbs in vivo, both can be macromolecular substances, also can be small-molecule substance, ion even, include but not limited to cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion, or the like.
In another preference, described cellulose comprises all celluloses, preferably microcrystalline cellulose, most preferably nano level cellulose.
In another preference, described fiber pentose is selected from following one or more: xylose (xylose), ribose (ribose), arabinose (arabinose), lyxose (lyxose), ribulose (ribulose), xylulose (xylulose).
In another preference, described fiber pentose obtains by following steps:
(a) will contain cellulosic biomass and water and mix, obtain liquid mixture I 20-180 ℃ of hydrolysis; With
(b) with liquid mixture I and distillery yeast 20-60 ℃ mix 6-48 hours after, the elimination solids concentrates and obtains the fiber pentose.
In another preference, described fiber pentose obtains by following steps:
(1) will contain cellulosic biomass, water and catalyst and mix 3-600 minutes at 30-180 ℃, hydrolysis obtains liquid mixture I; Described catalyst is selected from: hydrolysis of hemicellulose enzyme, organic acid, mineral acid or bionic catalyst; Described organic acid is selected from: contain the following organic acid of 20 carbon; Described mineral acid is selected from: sulphuric acid, hydrochloric acid, phosphoric acid; Described bionic catalyst is selected from [CpFeCO (maleic acid)] BF4 or second diamino oxalic acid iron complex;
(2) liquid mixture I and distillery yeast were mixed 6-48 hours at 20-60 ℃, obtain liquid mixture II; With
(3) liquid mixture ll obtains the fiber pentose through ion exchange.
In another preference, described fiber pentose contains arabinose 1-90wt% in fiber pentose gross weight; Contain arabinose 5-25wt% in the preferred described fiber pentose.
In another preference, described fiber pentose obtains by following steps:
(a) will contain cellulosic biomass and water and mix, obtain liquid mixture I 90-180 ℃ of hydrolysis; With
(b) liquid mixture I and distillery yeast were mixed 12-48 hours at 20-40 ℃, obtain the fiber pentose.
In another preference, described fiber pentose obtains by following steps:
(1) will contain cellulosic biomass, water and catalyst and mix 30-120 minutes at 90-180 ℃, hydrolysis obtains liquid mixture I; Described catalyst is selected from: hydrolysis of hemicellulose enzyme, organic acid, mineral acid or bionic catalyst; Described organic acid is selected from: contain the following organic acid of 20 carbon; Described mineral acid is selected from: sulphuric acid, hydrochloric acid, phosphoric acid; Described bionic catalyst is selected from: [CpFeCO (maleic acid)] BF4 or second diamino oxalic acid iron complex;
(2) liquid mixture I and distillery yeast were mixed 12-48 hours at 20-40 ℃, obtain liquid mixture II; With
(3) liquid mixture II obtains the fiber pentose through ion exchange.
In another preference, described food comprises solid food and liquid food.
In a second aspect of the present invention, provide a kind of and prepared the method for pure xylose from cellulose biomass, described method comprises step: with the fiber pentose through simple ethanol/water mixed solvent recrystallization;
Described fiber pentose obtains by following steps:
(1) will contain cellulosic biomass, water and catalyst and mix 30-120 minutes at 90-180 ℃, hydrolysis obtains liquid mixture I; Described catalyst is selected from: hydrolysis of hemicellulose enzyme, organic acid, mineral acid or bionic catalyst; Described organic acid is selected from: contain the following organic acid of 20 carbon; Described mineral acid is selected from: sulphuric acid, hydrochloric acid, phosphoric acid; Described bionic catalyst is selected from: [CpFeCO (maleic acid)] BF4 or second diamino oxalic acid iron complex;
(2) liquid mixture I and distillery yeast were mixed 12-48 hours at 20-40 ℃, obtain liquid mixture II; With
(3) liquid mixture II obtains the fiber pentose through ion exchange.
In view of the above, the invention provides a kind of material that contains natural pentose, it is the degree of absorption height in vivo, can effectively prevent and treat body acidification of diabetic, and production cost is low.
The specific embodiment
The inventor is through extensive and deep research, discovery will be by containing cellulosic biomass refine, can obtain the product of natural pentose: the fiber pentose, after it mixes with some materials, can improve the effective absorption in vivo of natural pentose greatly, thereby the product of sugared source energy can be provided for diabetics, prevent diabetics because of mainly using protein to cause the acidify of body for a long time, and then have the oxidative stress of reduction, prophylactic function as energy matter.
Definition
As used herein, " biomass of cellulose " and " containing cellulosic biomass " can exchange use, be meant that all contain cellulosic material, its scope comprises the cellulose biomass in the traditional definition, comprise seed, fruit, stem, leaf and the root of plant, even comprise some microorganisms.Described plant comprises that the characteristic of all nontoxic plants, product is not subjected to the influence of plant origin.All contain cellulosic biomass and all can use, the content of preferred hemicellulose is counted material more than the 5wt% with the cellulose biomass gross weight, and most preferably the content of hemicellulose is counted material more than the 10wt% with the cellulose biomass gross weight.
As used herein, " cellulose biomass " is meant with cellulose, hemicellulose and lignin to be the biomass of main component, generally do not comprise the fruit of plant.
Microcrystalline Cellulose is a kind of purified native cellulose depolymerization product, is the attritive powder shape, white, odorlessness, no taste; By forming by free-pouring crystalline powder (non-fibrous micropartical).Acid and most organic solvents of water insoluble, dilution are slightly soluble in 20% aqueous slkali.Dry powder sheeting be can be directly used in, drug excipient, flow aid, implant, disintegrating agent, antiplastering aid, adsorbent, capsule diluent etc. are widely used as.Nano micro crystal cellulose is meant that in the present invention cross-section lengths is the 10-20 nanometer, and fibre length is the cellulosic material less than 100 nanometers.
As used herein, the fiber pentose is selected from all natural pentoses, as xylose (xylose), ribose (ribose), arabinose (arabinose), the lyxose (lyxose) of five-carbon ring aldehydo sugar, the ribulose of five-carbon ketone sugar (ribulose), xylulose (xylulose), etc.The fiber pentose both can be single pure pentose, also can be their mixture, and the content of each sugar in mixture is not limit.In the present invention, " fiber pentose ", " natural pentose compositions " or " natural pentose " can exchange use, all are meant the monosaccharide that contains five carbon atoms or their the simple derivant or the polymer of the natural generation of all occurring in natures.
As used herein, " bionic catalyst " is meant the catalyst of the synthetic that contains a catalysis region (catalytic domain) and a cellulose binding domain (cellulose binding domain) at least, as [CpFeCO (maleic acid)] BF4, second diamino oxalic acid iron complex, or the like.Among patent application PCT/CN/2007/071114 bionic catalyst is had detailed description, wherein the bionic catalyst that is disclosed all can be used for preparation of product herein.
On the other hand, fiber pentose of the present invention can obtain by following step:
(a) will contain cellulosic biomass and water and mix, obtain liquid mixture I 20-180 ℃ of hydrolysis; With
(b) liquid mixture I and distillery yeast were mixed 6-48 hours at 20-60 ℃, the elimination solids concentrates and obtains the fiber pentose.
In a preferred embodiment of the present invention, obtain the fiber pentose by following steps:
The first step is used water (80-98 ℃ preferably are 90-95 ℃) dewaxing of closely boiling with the cellulosic biomass of pulverizing that contain;
Second step, contain cellulosic biomass and catalyst after the dewaxing to be mixed 3-600 minutes at 30-140 ℃, hydrolysis, solid-liquid separation obtain liquid mixture I; Described catalyst is selected from: hydrolysis of hemicellulose enzyme, organic acid, mineral acid or bionic catalyst; Described organic acid is selected from: contain the following organic acid of 20 carbon; Described mineral acid is selected from: sulphuric acid, hydrochloric acid, phosphoric acid; Described bionic catalyst is selected from: what patent application PCT/CN/2007/071114 disclosed;
In the 3rd step, separate or the deactivation catalyst; Described separation comprises passes through macroporous adsorbent resin with liquid mixture I; Described deactivation comprise heating, neutralization, or the like;
The 4th step, liquid mixture I and distillery yeast were mixed 6-48 hours at 20-60 ℃, the elimination solid obtains product liquid II;
In the 5th step, product liquid II through ion exchange, is obtained the fiber pentaglucose liquid.
Ion exchange in the 5th step comprises cation exchange and anion exchange, in order to improve the quality of product, also adds a mixed column that contains cationic resin and resin anion (R.A.) simultaneously sometimes.Eluting generally all is to use pure water, and the regeneration of exchanger resin generally all is sodium hydrate aqueous solution and dilute hydrochloric acid.
Contain fiber pentose and pentose absorption enhancer in the compositions of control body acidification of diabetic provided by the invention; Described sugared absorption enhancer is selected from one or more following materials: cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion.The fiber pentose wherein and the weight ratio of sugared absorption enhancer such as microcrystalline Cellulose are 0.001-1000:1, preferably are 0.015-10:1, more preferably are 0.02-0.7:1.
Compositions provided by the invention can be by with the fiber pentose be selected from following one or more materials and mix and obtain: cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion.Mix comprise conventional mixing, cocrystallization, altogether dry, or the like.
Content in compositions such as said vitamin, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion are the highest safe content that they allow.
Be used among the present invention to strengthen material that pentose absorbs comprise cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion, or the like.The content of said cellulose in compositions is between the 1%-99%.
In another preferred embodiment of the present invention, a kind of method of producing the present composition is provided, comprise the steps:
(a) the comminuted fibres cellulosic biomass uses the hot water dewaxing of closely boiling;
(b) cellulose biomass after the dewaxing is used for hydrolysis and prepares fiber pentose product, and hydrolysis is generally carried out in pure water, and hydrolyst is generally hydrolysis of hemicellulose enzyme, organic acid, mineral acid and bionic catalyst;
(c) solid-liquid separation obtains the solution of fibre-bearing pentose, separates or the deactivation catalyst;
(d) fermentation method is removed contained hexose;
(e) get fluid product after the solid-liquid separation;
(f) and the sugared absorption enhancer mix homogeneously of requirement obtain the product that needs.
Purposes
Fiber pentose provided by the invention or the compositions that contains the fiber pentose can be used for preparing the product that prevents and/or treats body acidification of diabetic, both can be normal food, health food or medicine.
Fiber pentose provided by the invention or the compositions that contains the fiber pentose can also be used to prepare the medicine that promotes that the fiber pentose absorbs, and especially can be used for preparing the medicine that promotes that arabinose absorbs.
Described medicine can be by with fiber pentose provided by the invention or contain the compositions of fiber pentose and pharmaceutically acceptable carrier mixes and obtains.
As used herein, term " pharmaceutically acceptable carrier " refers to be used for the treatment of the carrier of agent administration, comprises various excipient and diluent.This term refers to some medicament carriers like this: they itself are not necessary active component, and do not have undue toxicity after using.Suitable carriers is well known to those of ordinary skill in the art.(Mack Pub.Co. can find discussing fully about pharmaceutically acceptable excipient in N.J.1991) at Remington ' s PharmaceuticalSciences.Acceptable carrier can comprise liquid on combination of Chinese medicine is learned, as water, saline, glycerol and ethanol.In addition, also may there be complementary material in these carriers, as disintegrating agent, wetting agent, emulsifying agent, pH buffer substance etc.
Described medicine can be prepared into various dosage forms according to different way of administration, as solid preparation, or liquid preparation.These dosage forms are used in one of following mode: oral, spray suction, rectal application, nasal cavity applied medicine, buccal medication, local application, non-enterally administer, as in subcutaneous, vein, muscle, intraperitoneal, the sheath, in the ventricle, in the breastbone and intracranial injection or input, or by the medication of a kind of outer planting reservoir.
The invention provides a kind of acidifying product of diabetics health that prevents, concretely, provide a kind of monomer of natural pentose or the compositions between them, simultaneously, also provide a kind of pentose that can promote by the method that health absorbs, make diabetics still can use sugared source energy, assist to prevent the body acidification of diabetics, reduce the oxidative stress level, the prevent diabetes chronic complicating diseases.
Natural pentose is present in all containing in the cellulosic biomass basically, comprises in the cellulose biomass, comprise they seed, fruit, stem, leaf, root, or the like.The polymer of this fibrid pentose is a monosaccharide than facile hydrolysis in the manufacturing process of food, their metabolism in vivo do not need the participation of insulin, sound and the development of other function of health can be stimulated, the operating pressure of islets of langerhans can be alleviated simultaneously again, keeping the healthy extremely important of human body.But because so-called technological progress, accurately machined proportion is increasing among the human food, and the absorption of these natural pentoses significantly reduces.Though the mankind replenish managing again, as products such as preparation fruits vinegars in the hope of increasing the absorption of the natural pentose of health,, because the influence of human diet custom, the population that uses this series products is seldom.So, this natural pentose compositions is provided among the present invention, concerning general consumer, also possess important meaning.
Because natural arabinose shows it all is to absorb the poorest pentose in achievement in research up to now, the present invention is that example illustrates with natural arabinose.Should be understood that with natural arabinose to be that example only is to be used for better illustrating the present invention, rather than limit the scope of the invention.
The above-mentioned feature that the present invention mentions, or the feature that embodiment mentions can combination in any.All features that this case description is disclosed can with any composition forms and usefulness, each feature that is disclosed in the description can anyly provide the alternative characteristics of identical, impartial or similar purpose to replace.Therefore removing has special instruction, and the feature that is disclosed only is the general example of equalization or similar features.
Major advantage of the present invention is:
1, product provided by the invention effectively reduces the acidify of diabetics body;
2, product provided by the invention can recover custom orthobiosis of diabetics;
3, compositions provided by the invention can effectively promote the pentose absorption in vivo;
4, the present invention obtains the with low cost of natural pentose.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example is usually according to the normal condition or the condition of advising according to manufacturer.Unless otherwise indicated, otherwise all percent, ratio, ratio or umber by weight.
Unit in the percent weight in volume among the present invention is well-known to those skilled in the art, for example is meant the weight of solute in 100 milliliters solution.
Unless otherwise defined, the same meaning that employed all specialties and scientific words and one skilled in the art are familiar with in the literary composition.In addition, any method similar or impartial to described content and material all can be applicable in the inventive method.The usefulness that preferable implementation method described in the literary composition and material only present a demonstration.
Embodiment 1A prepares fiber pentose I
1000 gram (dry weight) chippy wheat straws (hemicellulose level is 21%) contact (for example using the hot water injection) after 10 minutes with hot water near boiling point, after pressing water purification to divide it as far as possible, transfer in 10 liters the pressure reaction still, bionic catalyst [CpFeCO (maleic acid)] BF4 (patent application PCT/CN/2007/071114) that adds 50mM then, stir down mixture heated to nearly 100oC and maintained this temperature about 60 minutes, behind the cool to room temperature, solid-liquid separation obtains fluid product.
Fluid product is by the XAD-4 macroporous adsorptive resins of 3cm * 60cm, flow velocity 2 ml/min, basically detect less than catalyst in the fiber pentose aqueous solution of gained, transferring pH then is that 5.5 backs add about 1.5% soybean cake powder, the 121oC sterilization adds 1% efficient Angel brewer's dried yeast, 34oC bottom fermentation 24 hours after 30 minutes.Sampling detects, hexose detect substantially less than, heating is filtered and is carried out solid-liquid separation after the yeast deactivation, fluid product.
Fluid product is crossed ion exchange (732-cation exchange resin column, 721-anion-exchange resin column and mixed bed ion exchange column), be concentrated to sugar content (total sugar content, comprise all pentoses, use abbe's refractometer, the liquid chromatograph accurate quantification) be about 50% aqueous solution, wherein the content of arabinose is about 12%, and the fluid product that obtains altogether is 260 milliliters, the HPLC chromatograph of this product only detects arabinose and xylose, and hexose does not observe.Repeat repeatedly to prepare, standby to obtain enough solid products.
Liquid chromatography HPLC is classical monosaccharide analytical method, use the LC-NH2 detached dowel (250X4.6mm) of SUPELCOSIL, mobile phase is acetonitrile: water=3:1, flow velocity is 1 ml/min, RI-detector, the retention time of ribose about 5.8 minutes, the retention time of xylose about 6.6 minutes, the retention time of arabinose about 7.3 minutes, the retention time of fructose about 8.2 minutes, the retention time of glucose about 9.7 minutes, the retention time of galactose is about 10.2 minutes.
Prepare solid product if desired, can adopt lyophilizing (lyophilization gets solid product 135 grams, and water content is 2%) or spray drying method to make; Prepare fluid product if desired, then get final product in inert gas shielding lower seal packing.
Embodiment 1B
1000 gram (dry weight) chippy corn brans, after adding pure water and being formulated as 5% mixture, transfer in 10 liters the pressure reaction still, bionic catalyst [CpFeCO (the maleic acid)] BF4 that adds 50mM then, stir down mixture heated to nearly 100oC and maintained this temperature about 60 minutes, behind the cool to room temperature, solid-liquid separation obtains fluid product.
Fluid product is by the XAD-4 macroporous adsorptive resins of 3cm * 60cm, flow velocity 2 ml/min, basically detect less than catalyst in the fiber pentose aqueous solution of gained, transferring pH then is that 5.5 backs add about 1.5% soybean cake powder, the 121oC sterilization adds 1% efficient Angel brewer's dried yeast, 34oC bottom fermentation 24 hours after 30 minutes.Sampling detects, HPLC analytical method hexose detect substantially less than, heating is filtered and is carried out solid-liquid separation after the yeast deactivation, fluid product.
Fluid product is crossed ion exchange (732-cation exchange resin column, 721-anion-exchange resin column and mixed bed ion exchange column), be concentrated to sugar content (total sugar content, use abbe's refractometer) be about 50%, wherein the content of arabinose is about 53%, and the fluid product that obtains altogether (being the fiber pentose) is 380 milliliters.
Embodiment 2
The fiber pentose solid product (being fiber pentose I) and the microcrystalline Cellulose of lyophilization preparation are mixed, the content that makes microcrystalline Cellulose is 10%, 20%, 30%, 40%, come half years old big monkey of feeding with them, the living environment of monkey is manually operated 12 hours daytime/12 hour nights, room temperature is 24 ℃, the normal food of feeding is after 7 days, be impregnated in the adding microcrystalline Cellulose in the food, the fiber pentose that does not add microcrystalline Cellulose, make the amount that contains arabinose in the food be respectively 20,40,60,80 gram/kilogram feedstuffs, collect the urinalysis arabinose content wherein of animal, the result is as follows:
| Feedstuff contains the arabinose amount | Microcrystalline cellulose cellulose content 0 | Microcrystalline cellulose cellulose content 10% | Microcrystalline cellulose cellulose content 20% | Microcrystalline cellulose cellulose content 30% | Microcrystalline cellulose cellulose content 40% |
| 20 | 10.1 | 6.3 | 4.6 | 3.3 | 1.1 |
| 40 | 12.3 | 7.5 | 5.2 | 3.8 | 1.7 |
| 60 | 14.7 | 8.2 | 5.9 | 4.3 | 1.9 |
| 80 | 15.5 | 8.9 | 6.4 | 4.9 | 2.1 |
(unit is a percentage ratio, and promptly how many arabinose in the food has excreted.Voided volume in 24 hours) result shows, unabsorbed arabinose is discharged by urine, and its output lowers along with increasing of microcrystalline cellulose cellulose content.
Embodiment 3
By the experimental program identical, show that during no microcrystalline Cellulose, when the amount of use vitamin B1 was 200 mg/kg fiber pentoses, the absorption of fiber pentose can increase by 16% with embodiment 2; When vitamin B5 was 200 mg/kg fiber pentoses, the absorption of fiber pentose can increase by 21%; When vitamin B6 was 200 mg/kg fiber pentoses, the absorption of fiber pentose can increase by 11%; When thyroxine was 30 mg/kg fiber pentoses, the absorption of fiber pentose can increase by 9%; When epinephrine was 20 mg/kg fiber pentoses, the absorption of fiber pentose can increase by 11%; When potassium ion was 200 mg/kg fiber pentoses, the absorption of fiber pentose can increase by 27%; When magnesium ion was 200 mg/kg fiber pentoses, the absorption of fiber pentose can increase by 16%; When sodium ion was 200 mg/kg fiber pentoses, the absorption of fiber pentose can increase by 6%.
Embodiment 4A
Use 9 week of II-type diabetic mice of streptozotocin preparation big, first group is matched group, 6, feeding pure protein food, and uses the metformin blood sugar control; Use the fiber pentose that contains microcrystalline Cellulose among the embodiment 2 in the second histone feedstuff, arabinose content in the feedstuff is 80 gram/kilograms, the content of microcrystalline Cellulose in fiber pentose product is 40%, behind the feeding three months, measure (get blood from the tail vein, add anticoagulant after, after centrifugal clear liquid, the Accurate pH instrumentation is fixed) acid-base value of their blood, the result is as follows:
| 1 | 2 | 3 | 4 | 5 | 6 | |
| First group | pH＝6.56 | pH＝6.63 | pH＝6.49 | pH＝6.52 | pH＝6.73 | pH＝6.76 |
| Second group | pH＝7.05 | pH＝7.04 | pH＝7.11 | pH＝7.05 | pH＝7.06 | pH＝7.02 |
The result shows, the product that is disclosed among the present invention can effectively prevent the acidify of diabetes body.
Embodiment 4B
Under nitrogen protection; fiber pentose solid product (being fiber pentose I) 800 grams with the lyophilization preparation; join in 3 liters 95% the edible ethanol; reflux slowly adds and is about 100 milliliters pure water, after refluxing about 10 minutes then; slowly be cooled to 20 ℃; after adding a small amount of xylose crystal seed, this temperature lower seal was deposited 24 hours, during rock gently once in a while.After 24 hours, be cooled to 0 ℃ of zero degree, put 48 hours, filter, dry under the vacuum, get the pure xylose of 500 grams, purity is 98.6%.Use 9 week of II-type diabetic mice of streptozotocin preparation big, first group is matched group, 6, feeding pure protein food, and uses the metformin blood sugar control; Use the pure xylose of above-mentioned preparation in the second histone feedstuff, xylose content in the feedstuff is 120 gram/kilograms, do not contain the microcrystalline Cellulose that adds, behind the feeding three months, measure (get blood from the tail vein, add anticoagulant after, after centrifugal clear liquid, the Accurate pH instrumentation is fixed) acid-base value of their blood, the result is as follows:
| 1 | 2 | 3 | 4 | 5 | 6 | |
| First group | pH=6.66 | pH-6.53 | pH=6.42 | pH=6.53 | pH-6.71 | pH＝6.69 |
| Second group | pH=7.01 | pH=6.94 | pH=7.03 | pH=6.97 | pH=7.02 | pH＝6.92 |
The result shows that pure pentose also possesses the function that prevents glycosuria sick body body acidification.
All quote in this application as a reference at all documents that the present invention mentions, just quoted as a reference separately as each piece document.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after having read above-mentioned teachings of the present invention, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Claims (13)
1. a fiber pentose or contain the purposes of the compositions of fiber pentose is characterized in that, is used to prepare the food or the medicine that prevent and/or treat body acidification of diabetic.
2. purposes as claimed in claim 1 is characterized in that, the described compositions that contains the fiber pentose is made up of the material that fiber pentose and promotion fiber pentose absorb in vivo.
3. purposes as claimed in claim 2 is characterized in that, in 100 weight portion compositionss, it contains:
1-100 weight portion fiber pentoses; With
0-99 weight portions sugar absorption enhancer; Described sugared absorption enhancer is selected from one or more following materials: cellulose, vitamin B1, vitamin B5, vitamin B6, thyroxine, epinephrine, potassium ion, magnesium ion, sodium ion.
4. purposes as claimed in claim 3 is characterized in that, the weight ratio of described fiber pentose and sugared absorption enhancer is 0.001-1000:1, preferably is 0.015-10:1, more preferably is 0.02-0.7:1.
5. purposes as claimed in claim 3 is characterized in that described cellulose comprises all celluloses, preferably microcrystalline cellulose, most preferably nano level cellulose.
6. as the arbitrary described purposes of claim 1-4, it is characterized in that described fiber pentose is selected from following one or more: xylose (xylose), ribose (ribose), arabinose (arabinose), lyxose (lyxose), ribulose (ribulose), xylulose (xylulose).
7. purposes as claimed in claim 6 is characterized in that, described fiber pentose contains arabinose 1-90wt% in fiber pentose gross weight; Contain arabinose 5-25wt% in the preferred described fiber pentose.
8. as the arbitrary described purposes of claim 1-4, it is characterized in that described fiber pentose obtains by following steps:
(a) will contain cellulosic biomass and water and mix, obtain liquid mixture I 20-180 ℃ of hydrolysis; With
(b) with liquid mixture I and distillery yeast 20-60 ℃ mix 6-48 hours after, the elimination solids concentrates and obtains the fiber pentose.
9. purposes as claimed in claim 8 is characterized in that, described fiber pentose obtains by following steps:
(1) will contain cellulosic biomass, water and catalyst and mix 3-600 minutes at 30-180 ℃, hydrolysis obtains liquid mixture I; Described catalyst is selected from: hydrolysis of hemicellulose enzyme, organic acid, mineral acid or bionic catalyst; Described organic acid is selected from: contain the following organic acid of 20 carbon; Described mineral acid is selected from: sulphuric acid, hydrochloric acid, phosphoric acid; Described bionic catalyst is selected from [CpFeCO (maleic acid)] BF4 or second diamino oxalic acid iron complex;
(2) liquid mixture I and distillery yeast were mixed 6-48 hours at 20-60 ℃, obtain liquid mixture II; With
(3) liquid mixture II obtains the fiber pentose through ion exchange.
10. purposes as claimed in claim 8 is characterized in that, described fiber pentose obtains by following steps:
(a) will contain cellulosic biomass and water and mix, obtain liquid mixture I 90-180 ℃ of hydrolysis; With
(b) liquid mixture I and distillery yeast were mixed 12-48 hours at 20-40 ℃, obtain the fiber pentose.
11. purposes as claimed in claim 8 is characterized in that, described fiber pentose obtains by following steps:
(1) will contain cellulosic biomass, water and catalyst and mix 30-120 minutes at 90-180 ℃, hydrolysis obtains liquid mixture I; Described catalyst is selected from: hydrolysis of hemicellulose enzyme, organic acid, mineral acid or bionic catalyst; Described organic acid is selected from: contain the following organic acid of 20 carbon; Described mineral acid is selected from: sulphuric acid, hydrochloric acid, phosphoric acid; Described bionic catalyst is selected from: [CpFeCO (maleic acid)] BF4 or second diamino oxalic acid iron complex;
(2) liquid mixture I and distillery yeast were mixed 12-48 hours at 20-40 ℃, obtain liquid mixture II; With
(3) liquid mixture II obtains the fiber pentose through ion exchange.
12. purposes as claimed in claim 1 is characterized in that, described food comprises solid food and liquid food.
13. one kind prepares the method for pure xylose from cellulose biomass, it is characterized in that described method comprises step: with the fiber pentose through simple ethanol/water mixed solvent recrystallization;
Described fiber pentose obtains by following steps:
(1) will contain cellulosic biomass, water and catalyst and mix 30-120 minutes at 90-180 ℃, hydrolysis obtains liquid mixture I; Described catalyst is selected from: hydrolysis of hemicellulose enzyme, organic acid, mineral acid or bionic catalyst; Described organic acid is selected from: contain the following organic acid of 20 carbon; Described mineral acid is selected from: sulphuric acid, hydrochloric acid, phosphoric acid; Described bionic catalyst is selected from: [CpFeCO (maleic acid)] BF4 or second diamino oxalic acid iron complex;
(2) liquid mixture I and distillery yeast were mixed 12-48 hours at 20-40 ℃, obtain liquid mixture II; With
(3) liquid mixture II obtains the fiber pentose through ion exchange.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910127799.XA CN101507728B (en) | 2009-03-24 | 2009-03-24 | Composite for preventing and treating body acidification of diabetic |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910127799.XA CN101507728B (en) | 2009-03-24 | 2009-03-24 | Composite for preventing and treating body acidification of diabetic |
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| Publication Number | Publication Date |
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| CN101507728A true CN101507728A (en) | 2009-08-19 |
| CN101507728B CN101507728B (en) | 2014-06-11 |
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| CN200910127799.XA Active CN101507728B (en) | 2009-03-24 | 2009-03-24 | Composite for preventing and treating body acidification of diabetic |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP1645636B1 (en) * | 2000-02-01 | 2008-06-11 | Unitika, Ltd. | A diabetic food and process for producing same |
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