WO2010108042A3 - Non-coherent light microscopy - Google Patents

Non-coherent light microscopy Download PDF

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Publication number
WO2010108042A3
WO2010108042A3 PCT/US2010/027872 US2010027872W WO2010108042A3 WO 2010108042 A3 WO2010108042 A3 WO 2010108042A3 US 2010027872 W US2010027872 W US 2010027872W WO 2010108042 A3 WO2010108042 A3 WO 2010108042A3
Authority
WO
WIPO (PCT)
Prior art keywords
coherent light
probe molecules
luminescence
sample
light source
Prior art date
Application number
PCT/US2010/027872
Other languages
French (fr)
Other versions
WO2010108042A2 (en
Inventor
Brian Thomas Bennett
Joerg Bewersdorf
Erik Jorgensen
Sam Hess
Travis Gould
Mudalige Siyath Gunewardene
Original Assignee
University Of Utah Research Foundation
The Jackson Laboratory
University Of Maine System Board Of Trustees
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Utah Research Foundation, The Jackson Laboratory, University Of Maine System Board Of Trustees filed Critical University Of Utah Research Foundation
Priority to JP2012500976A priority Critical patent/JP2012521541A/en
Priority to EP10754137.7A priority patent/EP2409139A4/en
Publication of WO2010108042A2 publication Critical patent/WO2010108042A2/en
Publication of WO2010108042A3 publication Critical patent/WO2010108042A3/en
Priority to US13/236,509 priority patent/US20120287244A1/en

Links

Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/26—Electron or ion microscopes; Electron or ion diffraction tubes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361—Optical details, e.g. image relay to the camera or image sensor
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/02—Details
    • H01J37/22—Optical or photographic arrangements associated with the tube
    • H01J37/226—Optical arrangements for illuminating the object; optical arrangements for collecting light from the object
    • H01J37/228—Optical arrangements for illuminating the object; optical arrangements for collecting light from the object whereby illumination and light collection take place in the same area of the discharge
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/26—Electron or ion microscopes; Electron or ion diffraction tubes
    • H01J37/28—Electron or ion microscopes; Electron or ion diffraction tubes with scanning beams
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/1006—Beam splitting or combining systems for splitting or combining different wavelengths

Landscapes

  • Analytical Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Multimedia (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

An optical microscope (101) with heightened resolution and capable of providing three dimensional images is disclosed and described. The microscope (101) can include a sample stage (160) for mounting a sample having a plurality of probe molecules. At least one non-coherent light source (127) can be provided. At least one lens (140a, 140b) can be configured to direct a beam of light from the at least one non-coherent light source (127) toward the sample causing the probe molecules to luminesce. A camera (155) can be configured to detect luminescence from the probe molecules. A light beam path modification module (132, 150) can be configured to alter a path length of the probe molecule luminescence to allow camera luminescence detection at a plurality of object planes.
PCT/US2010/027872 2009-03-18 2010-03-18 Non-coherent light microscopy WO2010108042A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2012500976A JP2012521541A (en) 2009-03-18 2010-03-18 Non-coherent optical microscope
EP10754137.7A EP2409139A4 (en) 2009-03-18 2010-03-18 Non-coherent light microscopy
US13/236,509 US20120287244A1 (en) 2009-03-18 2011-09-19 Non-coherent light microscopy

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US16134609P 2009-03-18 2009-03-18
US61/161,346 2009-03-18
US17771409P 2009-05-13 2009-05-13
US61/177,714 2009-05-13

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/236,509 Continuation-In-Part US20120287244A1 (en) 2009-03-18 2011-09-19 Non-coherent light microscopy

Publications (2)

Publication Number Publication Date
WO2010108042A2 WO2010108042A2 (en) 2010-09-23
WO2010108042A3 true WO2010108042A3 (en) 2011-01-13

Family

ID=42740240

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/027872 WO2010108042A2 (en) 2009-03-18 2010-03-18 Non-coherent light microscopy

Country Status (4)

Country Link
US (1) US20120287244A1 (en)
EP (1) EP2409139A4 (en)
JP (1) JP2012521541A (en)
WO (1) WO2010108042A2 (en)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010108038A2 (en) 2009-03-18 2010-09-23 University Of Utah Research Foundation A microscopy system and method for creating three dimensional images using probe molecules
WO2011024560A1 (en) * 2009-08-28 2011-03-03 オリンパスメディカルシステムズ株式会社 Receiver system
US9494783B2 (en) * 2010-11-30 2016-11-15 Etaluma Inc. Compact, high-resolution fluorescence and brightfield microscope and methods of use
WO2012096619A1 (en) * 2011-01-12 2012-07-19 Applied Precision, Inc. Systems and methods for camera-based image processing in microscopy instruments
US8830302B2 (en) * 2011-08-24 2014-09-09 Lg Electronics Inc. Gesture-based user interface method and apparatus
US9961326B2 (en) * 2012-01-09 2018-05-01 Kla-Tencor Corporation Stereo extended depth of focus
GB2511483B (en) * 2013-01-15 2016-11-23 Coolled Ltd LED Illumination
US20140235948A1 (en) * 2013-02-19 2014-08-21 The Board Of Trustees Of The Leland Stanford Junior University Method for single-fiber microscopy using intensity-pattern sampling and optimization-based reconstruction
US9435993B2 (en) 2013-03-24 2016-09-06 Bruker Nano, Inc. Three dimensional microscopy imaging
JP6305703B2 (en) * 2013-08-07 2018-04-04 東芝メモリ株式会社 Image acquisition apparatus, image acquisition method, and defect inspection apparatus
WO2016178856A1 (en) 2015-05-01 2016-11-10 The Board Of Regents Of The University Of Texas System Uniform and scalable light-sheets generated by extended focusing
US10871447B2 (en) * 2015-06-30 2020-12-22 Imec Vzw Bleaching of dyes in luminescent detection
EP3382441A4 (en) * 2015-11-27 2019-08-07 Nikon Corporation Microscope, observation method, and control program
US10783697B2 (en) 2016-02-26 2020-09-22 Yale University Systems, methods, and computer-readable media for ultra-high resolution 3D imaging of whole cells
WO2017180680A1 (en) 2016-04-12 2017-10-19 The Board Of Regents Of The University Of Texas System LIGHT-SHEET MICROSCOPE WITH PARALLELIZED 3D lMAGE ACQUISITION
CN105973897B (en) * 2016-06-14 2018-08-28 中国工程物理研究院激光聚变研究中心 The measurement method of the needle-shaped impaired loci geometric dimension distribution of KDP crystal
EP3538941A4 (en) 2016-11-10 2020-06-17 The Trustees of Columbia University in the City of New York Rapid high-resolution imaging methods for large samples
US11214795B2 (en) 2017-09-25 2022-01-04 California Institute Of Technology Bistable polynucleotide devices for the sensing and quantification of molecular events
US11391734B2 (en) 2017-09-25 2022-07-19 California Institute Of Technology Surface-immobilized bistable polynucleotide devices for the sensing and quantification of molecular events
EP3759468A4 (en) * 2018-03-01 2021-12-08 The Regents Of The University Of Colorado Methods and systems for stimulated emission depletion microscopy
WO2020013208A1 (en) * 2018-07-09 2020-01-16 国立大学法人神戸大学 Holographic three-dimensional multi-spot light stimulation device and method
EP3845970A4 (en) * 2018-08-29 2021-10-20 University of Hyogo Holographic imaging device and holographic imaging method
EP3812744A1 (en) * 2019-10-24 2021-04-28 Centre National de la Recherche Scientifique Apparatus for performing photochemical measurements on a liquid or liquid-containing sample
CN111912796B (en) * 2019-12-12 2023-03-10 南开大学 Super-resolution imaging system and method based on visible absorption spectrum
US11740180B2 (en) * 2020-09-28 2023-08-29 Purdue Research Foundation Method of measuring diffusion in a medium
US11733537B2 (en) * 2021-09-03 2023-08-22 Wisconsin Alumni Research Foundation Super-resolution optical microscope

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6444992B1 (en) * 1998-09-30 2002-09-03 Trellis Bioscience, Inc. High throughput microscopy
US6804385B2 (en) * 2000-10-24 2004-10-12 Oncosis Method and device for selectively targeting cells within a three-dimensional specimen
US20080085550A1 (en) * 2006-10-06 2008-04-10 The Regents Of The University Of California Apparatus and method for tracking a molecule or particle in three dimensions

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5532304A (en) * 1978-08-28 1980-03-07 Internatl Precision Inc Electron microscope with optical microscope
GB9024176D0 (en) * 1990-11-07 1990-12-19 Medical Res Council Photolabile compounds,their synthesis and use as fluorophores
JP3093145B2 (en) * 1995-12-13 2000-10-03 科学技術振興事業団 Light irradiation switching method
WO1997043611A1 (en) * 1996-05-16 1997-11-20 Affymetrix, Inc. Systems and methods for detection of labeled materials
JP4397993B2 (en) * 1999-03-24 2010-01-13 オリンパス株式会社 Photomicroscope
JP2001013413A (en) * 1999-07-02 2001-01-19 Nikon Corp Microscope
JP2003083894A (en) * 2001-09-14 2003-03-19 Sumitomo Electric Ind Ltd Method, device, and program for fluorescence intensity correction, and medium storing the program
US7180660B2 (en) * 2002-02-04 2007-02-20 Carl-Zeiss-Stiftung Trading As Carl Zeiss Stereo-examination systems and stereo-image generation apparatus as well as a method for operating the same
GB0211068D0 (en) * 2002-05-14 2002-06-26 Amersham Biosciences Uk Ltd Method for assessing biofilms
JP2004201526A (en) * 2002-12-24 2004-07-22 Toshiba Corp Method for detecting residual property of test substance in body, nucleic acid, protein, cell and probe-immobilizing chip
WO2004077021A2 (en) * 2003-02-27 2004-09-10 Lesko Stephen A Standardized evaluation of therapeutic efficacy based on cellular biomarkers
JP4563699B2 (en) * 2003-03-13 2010-10-13 オリンパス株式会社 Lighting switching device
DE102004015587A1 (en) * 2003-04-04 2004-11-11 Olympus Corporation Fluorescence microscope with total internal reflection
JP4683853B2 (en) * 2003-04-04 2011-05-18 オリンパス株式会社 Total reflection fluorescence microscope
JP2005188999A (en) * 2003-12-24 2005-07-14 Matsushita Electric Ind Co Ltd Particular component concentration measuring apparatus and particular component concentration measuring method
DE102004034977A1 (en) * 2004-07-16 2006-02-02 Carl Zeiss Jena Gmbh Scanning microscope and use
JP4722464B2 (en) * 2004-12-03 2011-07-13 オリンパス株式会社 Total reflection fluorescent lighting device
US7796797B2 (en) * 2005-09-28 2010-09-14 Sysmex Corporation Apparatus for obtaining an image of a blood cell and method for obtaining an image of a blood cell
JP4572162B2 (en) * 2005-11-22 2010-10-27 株式会社ジーオングストローム Microscope equipment
DE102006021317B3 (en) * 2006-05-06 2007-10-11 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Sample`s interesting structure spatial high-resolution imaging method, involves adjusting intensity of signal such that ten percentage of molecules of substance have specific distance to adjacent molecules
JP2008003053A (en) * 2006-06-26 2008-01-10 Olympus Corp Fluorescence observation or fluorescence photometry system, and method thereof
US7450243B2 (en) * 2006-07-10 2008-11-11 The Board Of Trustees Of The University Of Illinois Volumetric endoscopic coherence microscopy using a coherent fiber bundle
US7776613B2 (en) * 2006-08-07 2010-08-17 President And Fellows Of Harvard College Sub-diffraction image resolution and other imaging techniques
WO2008069220A1 (en) * 2006-11-30 2008-06-12 Nikon Corporation Imaging device and microscope
JP4851367B2 (en) * 2007-03-05 2012-01-11 富士フイルム株式会社 Local plasmon enhanced fluorescence sensor
JP5189301B2 (en) * 2007-03-12 2013-04-24 オリンパス株式会社 Laser scanning microscope
US20090017449A1 (en) * 2007-06-20 2009-01-15 President And Fellows Of Harvard College Compounds and methods for assaying fusion of an individual, enveloped virus with target membrane
EP2191254B1 (en) * 2007-08-31 2017-07-19 The General Hospital Corporation System and method for self-interference fluorescence microscopy, and computer-accessible medium associated therewith
US7777185B2 (en) * 2007-09-25 2010-08-17 Ut-Battelle, Llc Method and apparatus for a high-resolution three dimensional confocal scanning transmission electron microscope
US8456725B2 (en) * 2008-03-26 2013-06-04 Yale University Optical system that selectively provides either of a collimated light beam or a convergent light beam
US7675045B1 (en) * 2008-10-09 2010-03-09 Los Alamos National Security, Llc 3-dimensional imaging at nanometer resolutions

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6444992B1 (en) * 1998-09-30 2002-09-03 Trellis Bioscience, Inc. High throughput microscopy
US6804385B2 (en) * 2000-10-24 2004-10-12 Oncosis Method and device for selectively targeting cells within a three-dimensional specimen
US20080085550A1 (en) * 2006-10-06 2008-04-10 The Regents Of The University Of California Apparatus and method for tracking a molecule or particle in three dimensions

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
"Three-dimensional sub-100 nm resolution fluorescence microscopy.", 11 May 2008 (2008-05-11), XP002523348, Retrieved from the Internet <URL:http://www.nature.com/naturemethods> *
See also references of EP2409139A4 *

Also Published As

Publication number Publication date
EP2409139A4 (en) 2016-10-12
JP2012521541A (en) 2012-09-13
EP2409139A2 (en) 2012-01-25
WO2010108042A2 (en) 2010-09-23
US20120287244A1 (en) 2012-11-15

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