WO2010103530A1 - Procédé de production d'éthanol par une souche de saccharomyces qui fermente un sucre c5 - Google Patents

Procédé de production d'éthanol par une souche de saccharomyces qui fermente un sucre c5 Download PDF

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Publication number
WO2010103530A1
WO2010103530A1 PCT/IN2009/000491 IN2009000491W WO2010103530A1 WO 2010103530 A1 WO2010103530 A1 WO 2010103530A1 IN 2009000491 W IN2009000491 W IN 2009000491W WO 2010103530 A1 WO2010103530 A1 WO 2010103530A1
Authority
WO
WIPO (PCT)
Prior art keywords
fermentation
saccharomyces cerevisiae
sugar
mtcc
strain
Prior art date
Application number
PCT/IN2009/000491
Other languages
English (en)
Inventor
Samir S. Somaiya
Sangeeta Srivastava
Martin Annette
Original Assignee
Godavari Biorefineries Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Godavari Biorefineries Ltd. filed Critical Godavari Biorefineries Ltd.
Publication of WO2010103530A1 publication Critical patent/WO2010103530A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • Cellulose, hemicellulose are the most abundant renewable organic resources. These renewable organic resources can be used for the production of fuels like ethanol by the fermentation process using the easily available microorganisms like yeast.
  • the present invention makes available a Saccharomyces cerevisiae MTCC 5452 strain which is capable of further fermenting C-6 sugar to produce ethanol.
  • Figure 3 is a graph illustrating ethanol productivity by Saccharomyces cerevisiae strain MTCC 5452 as per example 6.
  • An exemplary preferred embodiment of the present invention method for producing ethanol by fermenting C-5 sugars with Saccharomyces cerevisiae MTCC 5452 comprises; incubating culture of Saccharomyces cerevisiae MTCC 5452 with a medium comprising atleast C-5 sugar carbon source under favourable pH and at temperature and fermentation conditions for a sufficient period to obtain ethanol; recovering ethanol from the fermentation broth by appropriate method.
  • the hemicellulose hydrolysate may be obtained by subjecting the hemicellulose fraction of the lignocellulose containing material to atleast one of the processes selected from acid hydrolysis or treatment with enzyme having hemicellulolytic activity.
  • hemicellulase can be added to degrade hemicellulose and obtain C-5 sugar containing hemicellulose hydrolysate.
  • the cellulose hydrolysate may be obtained by subjecting the cellulose fraction of lignocellulose containing material to at least one of the process selected from acid hydrolysis or treatment with enzyme having cellulolytic activity.
  • cellulase can be added to degrade cellulose and obtain C-6 sugar containing cellulose hydrolysate.
  • the lignocellulose containing material may be any of the above-mentioned materials in treated or untreated from.
  • a skilled person can implement possibly necessary pretreatment steps without inventive effort and without undue experimentation. It is further contemplated that other means of facilitating the degradation of substrates can be used in the process, examples including, but not limited to mechanical disruption, ultrasonication, or steam and high-pressure pre-treatments.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne une souche de Saccharomyces cerevisiae MTCC 5452 capable de fermenter un sucre C5 qui n'est pas une souche génétiquement modifiée. La présente invention concerne également un procédé de fermentation pour produire de l'éthanol avec ladite souche, qui comprend l'incubation d'une culture de Saccharomyces cerevisiae MTCC 5452 avec un milieu de fermentation comprenant au moins une source carbonée de sucre C5 dans des conditions de pH, de température et de fermentation favorables pendant une durée suffisante, pour obtenir de l'éthanol avec une productivité plus élevée.
PCT/IN2009/000491 2009-03-09 2009-09-08 Procédé de production d'éthanol par une souche de saccharomyces qui fermente un sucre c5 WO2010103530A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN525MU2009 2009-03-09
IN525/MUM/2009 2009-03-09

Publications (1)

Publication Number Publication Date
WO2010103530A1 true WO2010103530A1 (fr) 2010-09-16

Family

ID=42727873

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2009/000491 WO2010103530A1 (fr) 2009-03-09 2009-09-08 Procédé de production d'éthanol par une souche de saccharomyces qui fermente un sucre c5

Country Status (1)

Country Link
WO (1) WO2010103530A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9551015B2 (en) 2009-07-10 2017-01-24 Dsm Ip Assets B.V. Fermentative production of ethanol from glucose, galactose and arabinose employing a recombinant yeast strain
WO2017091361A1 (fr) * 2015-11-24 2017-06-01 Poet Research, Inc. Utilisation d'oxygène dissous pour inhiber la production d'acide lactique pendant la propagation de la levure et/ou l'hydrolyse de biomasse lignocellulosique
CN110117549A (zh) * 2019-03-26 2019-08-13 华南农业大学 一种酵母菌培养用的发酵培养基及其制备方法
CN113186245A (zh) * 2020-01-14 2021-07-30 广东利世康低碳科技有限公司 一种耐受辣椒且同时利用葡萄糖和木糖发酵的酵母菌及其验证方法
CN115927155A (zh) * 2022-12-19 2023-04-07 天津科技大学 一种木质纤维素衍生抑制剂耐受菌株的筛选方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5151354A (en) * 1986-08-21 1992-09-29 Cornelius Hollenberg Fermentation processes using amylolytic enzyme producing microorganisms
US5693526A (en) * 1996-03-29 1997-12-02 Council Of Scientific & Industrial Research Strains of yeast of saccharomyces cerevisiae and a process for the preparation of such strains of yeast
US20060110609A1 (en) * 2004-11-19 2006-05-25 Eaton Harry E Protective coatings
US20090123979A1 (en) * 2007-11-01 2009-05-14 Novozymes, Inc. Methods of reducing the inhibitory effect of a tannin on the enzymatic hydrolysis of cellulosic material

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5151354A (en) * 1986-08-21 1992-09-29 Cornelius Hollenberg Fermentation processes using amylolytic enzyme producing microorganisms
US5693526A (en) * 1996-03-29 1997-12-02 Council Of Scientific & Industrial Research Strains of yeast of saccharomyces cerevisiae and a process for the preparation of such strains of yeast
US20060110609A1 (en) * 2004-11-19 2006-05-25 Eaton Harry E Protective coatings
US20090123979A1 (en) * 2007-11-01 2009-05-14 Novozymes, Inc. Methods of reducing the inhibitory effect of a tannin on the enzymatic hydrolysis of cellulosic material

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9551015B2 (en) 2009-07-10 2017-01-24 Dsm Ip Assets B.V. Fermentative production of ethanol from glucose, galactose and arabinose employing a recombinant yeast strain
US10260075B2 (en) 2009-07-10 2019-04-16 Dsm Ip Assets B.V. Fermentative production of ethanol from glucose, galactose and arabinose employing a recombinant yeast strain
US10982235B2 (en) 2009-07-10 2021-04-20 Dsm Ip Assets B.V. Fermentative production of ethanol from glucose, galactose and arabinose employing a recombinant yeast strain
US11649471B2 (en) 2009-07-10 2023-05-16 Dsm Ip Assets B.V. Fermentative production of ethanol from glucose, galactose and arabinose employing a recombinant yeast strain
WO2017091361A1 (fr) * 2015-11-24 2017-06-01 Poet Research, Inc. Utilisation d'oxygène dissous pour inhiber la production d'acide lactique pendant la propagation de la levure et/ou l'hydrolyse de biomasse lignocellulosique
CN108474015A (zh) * 2015-11-24 2018-08-31 波特研究公司 使用溶解氧抑制酵母的繁殖和/或木质纤维素生物质的水解过程中乳酸的产生
US10731191B2 (en) 2015-11-24 2020-08-04 Poet Research, Inc. Using dissolved oxygen to inhibit lactic acid production during propagation of yeast and/or hydrolysis of lignocellulosic biomass
US11597957B2 (en) 2015-11-24 2023-03-07 Poet Research, Inc. Using dissolved oxygen to inhibit lactic acid production during propagation of yeast and/or hydrolysis of lignocellulosic biomass
CN110117549A (zh) * 2019-03-26 2019-08-13 华南农业大学 一种酵母菌培养用的发酵培养基及其制备方法
CN113186245A (zh) * 2020-01-14 2021-07-30 广东利世康低碳科技有限公司 一种耐受辣椒且同时利用葡萄糖和木糖发酵的酵母菌及其验证方法
CN115927155A (zh) * 2022-12-19 2023-04-07 天津科技大学 一种木质纤维素衍生抑制剂耐受菌株的筛选方法

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