WO2010099394A9 - Altération et modulation de l'activité protéique en faisant varier la modification post-traductionnelle alteration and modulation of protein activity by varying post-translational modification - Google Patents

Altération et modulation de l'activité protéique en faisant varier la modification post-traductionnelle alteration and modulation of protein activity by varying post-translational modification Download PDF

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Publication number
WO2010099394A9
WO2010099394A9 PCT/US2010/025521 US2010025521W WO2010099394A9 WO 2010099394 A9 WO2010099394 A9 WO 2010099394A9 US 2010025521 W US2010025521 W US 2010025521W WO 2010099394 A9 WO2010099394 A9 WO 2010099394A9
Authority
WO
WIPO (PCT)
Prior art keywords
seq
enzyme
protein
purified
isolated
Prior art date
Application number
PCT/US2010/025521
Other languages
English (en)
Other versions
WO2010099394A1 (fr
Inventor
David N. Thompson
David W. Reed
Vicki S. Thompson
Jeffrey A. Lacey
William A. Apel
Original Assignee
Battelle Energy Alliance, Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/380,450 external-priority patent/US9234228B2/en
Priority claimed from US12/655,993 external-priority patent/US8969033B2/en
Application filed by Battelle Energy Alliance, Llc filed Critical Battelle Energy Alliance, Llc
Priority to CA2752175A priority Critical patent/CA2752175A1/fr
Priority to EP10746882A priority patent/EP2401289A4/fr
Priority to BRPI1008486A priority patent/BRPI1008486A2/pt
Priority to AU2010217847A priority patent/AU2010217847A1/en
Priority to CN2010800087950A priority patent/CN102317309A/zh
Publication of WO2010099394A1 publication Critical patent/WO2010099394A1/fr
Publication of WO2010099394A9 publication Critical patent/WO2010099394A9/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases

Abstract

Les modes de réalisation selon l'invention comprennent des procédés pour altérer l'activité enzymatique ou la solubilité d'une enzyme extrêmophile ou pour modifier après traduction une protéine d'intérêt au moyen de glycosyltransférases isolées ou partiellement purifiées et/ou de protéines de modification post-traductionnelles, d'extraits de cellules comprenant des glycosyltransférases et/ou des protéines de modification post-traductionnelles et/ou dans des cellules comprenant une ou plusieurs glycosyltransférases et/ou protéines de modification post-traductionnelles.
PCT/US2010/025521 2009-02-26 2010-02-26 Altération et modulation de l'activité protéique en faisant varier la modification post-traductionnelle alteration and modulation of protein activity by varying post-translational modification WO2010099394A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA2752175A CA2752175A1 (fr) 2009-02-26 2010-02-26 Alteration et modulation de l'activite proteique en faisant varier la modification post-traductionnelle alteration and modulation of protein activity by varying post-translational modification
EP10746882A EP2401289A4 (fr) 2009-02-26 2010-02-26 Altération et modulation de l'activité protéique en faisant varier la modification post-traductionnelle
BRPI1008486A BRPI1008486A2 (pt) 2009-02-26 2010-02-26 alteração e modulação de atividade de proteína por variação de modificação pós-traducional
AU2010217847A AU2010217847A1 (en) 2009-02-26 2010-02-26 Alteration and modulation of protein activity by varying post-translational modification
CN2010800087950A CN102317309A (zh) 2009-02-26 2010-02-26 通过不同的翻译后修饰改变和调节蛋白质活性

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US12/380,450 US9234228B2 (en) 2008-02-27 2009-02-26 Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
USPCT/US2009/035307 2009-02-26
US12/380,450 2009-02-26
PCT/US2009/035307 WO2010051049A2 (fr) 2008-02-27 2009-02-26 Gènes et enzymes de glycosylation thermophiles et thermoacidophiles provenant d'alicyclobacillus acidocaldarius et d'organismes apparentés, et procédés
US12/655,993 2010-01-12
US12/655,993 US8969033B2 (en) 2005-11-02 2010-01-12 Alteration and modulation of protein activity by varying post-translational modification

Publications (2)

Publication Number Publication Date
WO2010099394A1 WO2010099394A1 (fr) 2010-09-02
WO2010099394A9 true WO2010099394A9 (fr) 2011-10-13

Family

ID=42665924

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/025521 WO2010099394A1 (fr) 2009-02-26 2010-02-26 Altération et modulation de l'activité protéique en faisant varier la modification post-traductionnelle alteration and modulation of protein activity by varying post-translational modification

Country Status (6)

Country Link
EP (1) EP2401289A4 (fr)
CN (1) CN102317309A (fr)
AU (1) AU2010217847A1 (fr)
BR (1) BRPI1008486A2 (fr)
CA (1) CA2752175A1 (fr)
WO (1) WO2010099394A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160130324A1 (en) 2014-10-31 2016-05-12 Shire Human Genetic Therapies, Inc. C1 Inhibitor Fusion Proteins and Uses Thereof
WO2017087882A1 (fr) 2015-11-19 2017-05-26 Shire Human Genetic Therapies, Inc. Inhibiteur de la c1 estérase humaine recombinante et ses utilisations
CA3034627A1 (fr) * 2016-11-28 2018-05-31 C-Lecta Gmbh Trehalose phosphorylase
CN108192906B (zh) * 2017-12-28 2021-05-11 江苏世邦生物工程科技有限公司 一种含有低温脂肪酶基因的工程菌及其构建方法和应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006078821A2 (fr) * 2005-01-21 2006-07-27 Invitrogen Corporation Produits et procedes de synthese de biomolecules in vitro
US7727755B2 (en) * 2005-11-02 2010-06-01 Battelle Energy Alliance, Llc Enzyme and methodology for the treatment of a biomass
WO2010051049A2 (fr) * 2008-02-27 2010-05-06 Battelle Energy Alliance, Llc Gènes et enzymes de glycosylation thermophiles et thermoacidophiles provenant d'alicyclobacillus acidocaldarius et d'organismes apparentés, et procédés

Also Published As

Publication number Publication date
EP2401289A4 (fr) 2012-10-03
AU2010217847A1 (en) 2011-10-27
CA2752175A1 (fr) 2010-09-02
EP2401289A1 (fr) 2012-01-04
WO2010099394A1 (fr) 2010-09-02
BRPI1008486A2 (pt) 2018-01-16
CN102317309A (zh) 2012-01-11

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