WO2010090452A3 - 세포 내 전달능이 증가된 작은 간섭 rna 복합체 - Google Patents

세포 내 전달능이 증가된 작은 간섭 rna 복합체 Download PDF

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WO2010090452A3
WO2010090452A3 PCT/KR2010/000678 KR2010000678W WO2010090452A3 WO 2010090452 A3 WO2010090452 A3 WO 2010090452A3 KR 2010000678 W KR2010000678 W KR 2010000678W WO 2010090452 A3 WO2010090452 A3 WO 2010090452A3
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sirna
transmission capacity
nucleic acid
complex
functional nucleic
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WO2010090452A2 (ko
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이동기
장찬일
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성균관대학교산학협력단
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Priority to JP2011547817A priority Critical patent/JP5529895B2/ja
Priority to EP10738736.7A priority patent/EP2395085B1/en
Priority to US13/146,862 priority patent/US20120016007A1/en
Publication of WO2010090452A2 publication Critical patent/WO2010090452A2/ko
Publication of WO2010090452A3 publication Critical patent/WO2010090452A3/ko
Priority to US16/151,867 priority patent/US20190119672A1/en

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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Abstract

본 발명은 세포 내 전달능이 증가된 다중siRNA 복합체 및 다기능성 핵산구조 복합체에 관한 것이다. 본 발명에 따른 siRNA 복합체 및 다기능성 핵산구조 복합체는 종래 복수의 목적 유전자 발현을 억제하기 위한 shRNA 시스템에 비하여 용이하게 화학적 합성될 수 있는 새로운 구조이면서도, 종래의 siRNA 보다 향상된 효율로 복수의 유전자를 동시에 발현 억제시킬 수 있는 장점이 있다. 또한, 높은 세포 내 전달능을 가질 뿐만 아니라, 비특이적인 항바이러스성 반응을 일으킴없이 특이적으로 목적 유전자들을 발현 억제시킬 수 있는 바, 암이나 바이러스성 감염 등을 치료하기 위한 siRNA 기작 매개 치료제로서 매우 유용하다. 아울러, 본 발명에 따른 다기능성 핵산구조 복합체는, siRNA 이외에 miRNA, antagomiR, 안티센스 올리고뉴클레오티드, 압타머 및 라이보자임(ribozyme)과 같은 기능성 핵산올리고뉴클에오티드를 결합하여 동시에 다양한 기능을 제공할 수 있다.
PCT/KR2010/000678 2009-02-04 2010-02-04 세포 내 전달능이 증가된 작은 간섭 rna 복합체 WO2010090452A2 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2011547817A JP5529895B2 (ja) 2009-02-04 2010-02-04 細胞内の伝達能が増加した低分子干渉ｒｎａ複合体
EP10738736.7A EP2395085B1 (en) 2009-02-04 2010-02-04 Small interference rna complex with increased intracellular transmission capacity
US13/146,862 US20120016007A1 (en) 2009-02-04 2010-02-04 Small interference rna complex with increased intracellular transmission capacity
US16/151,867 US20190119672A1 (en) 2009-02-04 2018-10-04 Small interference rna complex with increased intracellular transmission capacity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20090009036 2009-02-04
KR10-2009-0009036 2009-02-04

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/146,862 A-371-Of-International US20120016007A1 (en) 2009-02-04 2010-02-04 Small interference rna complex with increased intracellular transmission capacity
US16/151,867 Continuation US20190119672A1 (en) 2009-02-04 2018-10-04 Small interference rna complex with increased intracellular transmission capacity

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WO2010090452A2 WO2010090452A2 (ko) 2010-08-12
WO2010090452A3 true WO2010090452A3 (ko) 2010-11-25

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PCT/KR2010/000678 WO2010090452A2 (ko) 2009-02-04 2010-02-04 세포 내 전달능이 증가된 작은 간섭 rna 복합체

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US (2) US20120016007A1 (ko)
EP (1) EP2395085B1 (ko)
JP (1) JP5529895B2 (ko)
KR (1) KR101169373B1 (ko)
WO (1) WO2010090452A2 (ko)

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JP7213897B2 (ja) * 2018-07-20 2023-01-27 グアンジョウ リボビオ カンパニー リミテッド 核酸ユニットとその高分子核酸及びその応用
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WO2020144583A1 (en) * 2019-01-08 2020-07-16 Olix Pharmaceuticals, Inc. Cell-penetrating tripodal interfering rna
JP7313790B2 (ja) 2019-08-02 2023-07-25 株式会社ハーモニック・ドライブ・システムズ ロータリアクチュエータ
CN114480377A (zh) * 2022-01-27 2022-05-13 浙江浥眸生物科技有限公司 一种刷状核酸组装体和复合纳米粒子及其应用
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Publication number Publication date
JP2012516683A (ja) 2012-07-26
KR101169373B1 (ko) 2012-07-30
WO2010090452A2 (ko) 2010-08-12
US20190119672A1 (en) 2019-04-25
EP2395085A9 (en) 2012-02-29
EP2395085A4 (en) 2013-01-23
US20120016007A1 (en) 2012-01-19
EP2395085B1 (en) 2015-06-10
JP5529895B2 (ja) 2014-06-25
KR20100089796A (ko) 2010-08-12
EP2395085A2 (en) 2011-12-14

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