WO2010082587A1

WO2010082587A1 - Adsorbent for suspension in air

Info

Publication number: WO2010082587A1
Application number: PCT/JP2010/050287
Authority: WO
Inventors: 三郎大原
Original assignee: 株式会社大気倶楽部; 亜東商事株式会社
Priority date: 2009-01-13
Filing date: 2010-01-13
Publication date: 2010-07-22

Abstract

Disclosed is an adsorbent for a suspension in air, which can inactivate an influenza virus in the suspension in air. Also disclosed is a method for inactivating an influenza virus. Further disclosed is a method for producing an adsorbent for a suspension in air, wherein the adsorbent can inactivate an influenza virus. Specifically disclosed is an adsorbent for a suspension in air, which can inactivate an influenza virus and comprises ethanol, a surfactant, polylysine, glycine and a food additive polymer. In the adsorbent for a suspension in air, water may be used as a solvent.

Description

Air suspension adsorbent

The present invention relates to a low-concentration ethanol preparation that is sprayed into an environment in which a suspension containing bacteria and viruses is suspended to sterilize or inactivate pathogenic microorganisms. In particular, it relates to an ethanol preparation that inactivates influenza virus.

Airborne suspensions contain pathogenic microorganisms such as brute force and bacteria mixed with dust, water droplets, pollen and exhaust gas. Among pathogenic microorganisms, there are pathogenic microorganisms that caused a pandemic typified by SARS that spread around the world in a few days from a single patient who entered Hong Kong from southern China, which became a global problem in 2002. In order to prevent infection with pathogenic microorganisms that cause serious air infections, it is necessary to take preventive measures such as developing safe usable drugs that can remove pathogenic microorganisms from the air.

Generally, in the air, gram-negative bacteria such as coliforms are vulnerable to drying and cannot float in the air for a long time, but Bacillus, mold spores, microknots, streptococci, etc. are strong in drying and long in the air. Float. Large mold spores float in the air as they are, but pathogenic bacteria and yeast float in the air as they are contained in clothes garbage and water droplets. Bacteria have many common bacteria such as cocci, bacilli and spore bacteria, and the ratio of cocci is high. There are a small number of molds such as Penicillim, Cladosporium, Aspergillus, Fusarium, Trichoderma, and other membrane-producing yeasts, Rhodetorufa (Non-patent Document 1).

The problem here is when SARS (coronavirus), the influenza A virus that repeats epidemic every winter, and the highly virulent avian influenza virus, etc. will be mutated to become a new influenza virus, and the outbreak will no longer occur There is no reliable preventive measure even though it is a time issue (Non-Patent Document 2).

The flu, avian flu, and new flu that are prevalent each year are the same influenza viruses. Originally duck inhabiting Siberia go south for wintering, fly to lakes and drop feces, but there are a tremendous number of viruses in the feces. Water birds such as duck do not become ill when infected with influenza A virus, and maintain viruses in nature. Most of these viruses are attenuated viruses. Even if these viruses infect chickens and the like, many remain insidious infections, but a large amount of viruses are present in the feces and are said to be a source of infection to other birds (Non-patent Document 2). .

In 1994, Kida et al. Examined the role that pigs played in the emergence of the A / Hong Kong / 68 (H3N2) strain that appeared as a new human virus in 1968. It became a place for gene segment exchange and revealed that pigs create new influenza viruses. Pigs infected with the virus tolerated without showing any clinical symptoms, and pathological examination showed no lesions. As a result of studying the ecological survey, the introduction route was reported as follows (Non-patent Document 3).

A / Hong Kong / 68 shares are introduced through the migratory duck group nesting in Siberia and Alaska in the summer, and wintering over Japan, China, Taiwan, Southeast Asia and North America in the fall. The duck flies to the pond of the farm where the house duck and the pig are raised together and excretes the influenza virus along with the feces. Here, humans and pigs live closely, so the virus travels back and forth between pigs and humans. As a result, H3 virus from duck and H2N2 virus from human Asian cold co-infect pigs, and gene segmentation is repeated. Aggregates were produced.

That is, it was concluded that the advent of A / Hong Kong / 68 strain played the role of migratory duck, Chinese domestic duck and pig, respectively, for virus gene supply, transmission and gene segment reassembly production (Non-patent Document 3).

Many research efforts have revealed the transmission route of the new influenza virus that infects humans, but why did it infect humans?
(1) When both avian and human viruses infect pigs at the same time, and both genes are mixed in the body of the pig, a new human flu outbreak from avian influenza is born.
(2) Avian influenza is mutated to acquire human transmission and transmission from person to person.
(3) Among people infected with both bird flu and human flu, viruses cross and new viruses are born.
(Non-Patent Document 2), and it is considered that all three of these viruses infect humans via a floating substance in the air.

The first thing to notice about the outbreak of a new influenza virus, which cannot be predicted when and where it occurs, is due to abnormalities in chickens in pig farms and pigs in pig farms. The main virus infection is air infection that infects people who have inhaled viruses in the air, and it is highly unlikely that bird viruses will infect humans, but there are many opportunities to come into contact with bird viruses. The infection rate is higher. In general, chickens infected with a weakly virulent virus propagate only in the respiratory tract and intestinal tract, and noticeable symptoms often do not appear, but chickens infected with a virulent virus are systemic infections such as multiple organ failure. Can cause death in 1-2 days. In order to prevent the spread of infection, all chickens suspected of being infected are promptly disposed of to reduce the amount of virus transmitted to humans and delay infection (Non-patent Document 2). It is well known that when a new type of influenza emerges, it is necessary to immediately determine the classification of the virus type, urgently produce the vaccine, and immediately supply and inoculate it.

However, since vaccines can only be developed after detecting the new influenza virus, it takes at least six months to be generally available. In addition, since the manufactured products are accompanied by strict quality control and the production capacity is limited, even if the supply of growing eggs essential for vaccine production goes smoothly, 1 is sufficient until a sufficient amount is secured. It is expected to take almost a year, and half a year after the advent of the new influenza virus will surpass the emergency without a vaccine.

In the meantime, the use of a mask prevents the inhalation of viruses and prevents the spread of droplet infections caused by coughing. Encourage hand washing, crawl, refrain from going out. In addition, there are drugs that prevent the spread of viral infections as antiviral drugs, but there is no data to support their effectiveness if they are not taken within 48 hours after onset, and a drug in which resistant viruses appear even if taken. There is also. In addition, drugs that suppress the action of sialidase (neuraminidase) on the surface of the virus and prevent germination and release of offspring viruses can prevent the spread of infection but cannot prevent infection.

In addition to this, there is a pre-pandemic vaccine that can be manufactured before the new epidemic, but since it was developed using the H5N1 virus as a model, a certain preventive effect can be expected if it is H5 type, but it will be invalid if the new virus is other than H5 There are pros and cons in terms of risks. In preparation for the emergence of new models, pre-vaccination was considered until vaccination was possible at the medical site, and clinical research was started by trial vaccination targeting 6400 people including medical workers, but more than 70% of vaccination persons It is necessary to have antibodies that are effective in preventing disease, and there is a theory that this ratio is 84% for UK-made vaccines, but there are doubts about the effectiveness of Japanese products at 17.4% (Non-patent Document 4).

An object of the present invention is to provide an air suspension adsorbent that inactivates influenza virus in an air suspension. For example, in the event of a new influenza outbreak overseas, even if people who are urgently trying to enter Japan, for example, become infected during flight and develop during flight, the virus will be prevented from spreading in the plane. In addition, the air suspension adsorbent that inactivates the influenza virus in the air suspension, which is effective for waterfront measures to normalize the air environment in advance and prevent the invasion of the new influenza virus, the influenza virus It is in providing the manufacturing method of the air suspension adsorbent which inactivates the method of inactivation, and inactivates influenza virus.

According to one embodiment of the present invention, an air suspension adsorbent is provided that inactivates influenza virus comprising ethanol, surfactant, polylysine, glycine, and food additive polymer.

In the air suspension adsorbent that inactivates influenza virus, water may be used as a solvent for the air suspension adsorbent.

In the air suspension adsorbent that inactivates influenza virus, the surfactant is any of glycerin fatty acid ester, citric acid fatty acid monoglyceride, succinic acid fatty acid monoglyceride, diacetyltartaric acid fatty acid monoglyceride, diglycerin fatty acid ester, and sucrose fatty acid ester. One or a combination of two or more may be selected.

In the air suspension adsorbent that inactivates influenza virus, the glycerin fatty acid ester, the citric acid fatty acid monoglyceride, the succinic acid fatty acid monoglyceride, and the diacetyltartaric acid fatty acid monoglyceride have 8, 10, 12, 18 carbon atoms in the fatty acid. You may choose from either.

In the air suspension adsorbent that inactivates the influenza virus, the diglycerin fatty acid ester may have 14 fatty acids.

In the air suspension adsorbent that inactivates influenza virus, the sucrose fatty acid ester may be selected from any of 12, 16, and 18 carbon atoms of the fatty acid.

In the air suspension adsorbent that inactivates influenza virus, the food additive polymer may be selected from any of sodium polyacrylate, sodium carboxymethylcellulose, regenerated cellulose porous membrane, and polydextrose.

In the air suspension adsorbent that inactivates influenza virus, the air suspension adsorbent may further contain a complex carbohydrate sugar chain.

In the air suspension adsorbent that inactivates influenza virus, the complex carbohydrate sugar chain may be N-acetylneuraminic acid and galactose.

In the air suspension adsorbent that inactivates influenza virus, the air suspension adsorbent may further contain a fragrance.

Also, according to one embodiment of the present invention, there is provided a method for inactivating influenza virus by spraying an air suspension adsorbent comprising ethanol, surfactant, polylysine, glycine, and food additive polymer. .

In the method for inactivating influenza virus, the first solution containing the ethanol and the food additive polymer and the second solution containing the ethanol, the surfactant, the polylysine and the glycine are sprayed, respectively. The first solution and the second solution may be mixed in the air.

In the method for inactivating influenza virus, water may be used as a solvent for the air suspension adsorbent.

In the method for inactivating influenza virus, the surfactant is any one of glycerin fatty acid ester, citrate fatty acid monoglyceride, succinic acid fatty acid monoglyceride, diacetyltartaric acid fatty acid monoglyceride, diglycerin fatty acid ester, sucrose fatty acid ester, or Two or more combinations may be selected.

In the method for inactivating influenza virus, the glycerin fatty acid ester, the citrate fatty acid monoglyceride, the succinic acid fatty acid monoglyceride, and the diacetyltartaric acid monoglyceride are selected from any of 8, 10, 12, and 18 carbon atoms of fatty acid. May be.

In the method for inactivating influenza virus, the diglycerin fatty acid ester may have 14 fatty acids.

In the method for inactivating influenza virus, the sucrose fatty acid ester may be selected from any of 12, 16, and 18 carbon atoms of the fatty acid.

In the method of inactivating influenza virus, the food additive polymer may be selected from any of polyacrylic acid sodium salt, carboxymethylcellulose sodium, regenerated cellulose porous membrane, and polydextrose.

In the method for inactivating influenza virus, the air suspension adsorbent may further contain a complex carbohydrate chain.

In the method for inactivating influenza virus, the complex carbohydrate sugar chain may be N-acetylneuraminic acid and galactose.

In the method for inactivating influenza virus, the air suspension adsorbent may further include a fragrance.

According to one embodiment of the present invention, water is heated to 46 ° C. or more and 67 ° C. or less, and a food additive polymer, glycine and polylysine are added and dissolved to prepare a first solution, and the surfactant The suspension is adsorbed in air to inactivate influenza virus, wherein the second solution is prepared by dissolving ethanol by adding ethanol to the first solution, and the second solution is dissolved in the first solution. A method for producing the agent is provided.

In the method for producing an air suspension adsorbent that inactivates influenza virus, a complex carbohydrate sugar chain may be further added to the first solution.

In the method for producing an air suspension adsorbent that inactivates influenza virus, the surfactant is glycerin fatty acid ester, citric acid fatty acid monoglyceride, succinic acid fatty acid monoglyceride, diacetyltartaric acid fatty acid monoglyceride, diglycerin fatty acid ester, sucrose fatty acid. Any one of esters or a combination of two or more may be selected.

In the method for producing an air suspension adsorbent that inactivates influenza virus, the glycerin fatty acid ester, the citrate fatty acid monoglyceride, the succinic acid fatty acid monoglyceride, and the diacetyltartaric acid fatty acid monoglyceride have 8, 10 and 12 carbon atoms in the fatty acid. , 18 may be selected.

In the method for producing an air suspension adsorbent that inactivates influenza virus, the diglycerin fatty acid ester may have 14 fatty acids.

In the method for producing an air suspension adsorbent that inactivates influenza virus, the sucrose fatty acid ester may be selected from any of 12, 16, and 18 carbon atoms of the fatty acid.

In the method for producing an air suspension adsorbent that inactivates influenza virus, the food additive polymer is selected from any of polyacrylic acid sodium salt, sodium carboxymethylcellulose, regenerated cellulose porous membrane, and polydextrose Also good.

In the method for producing an air suspension adsorbent that inactivates influenza virus, the complex carbohydrate sugar chain may be N-acetylneuraminic acid and galactose.

In the method for producing an air suspension adsorbent that inactivates influenza virus, the air suspension adsorbent may further contain a fragrance.

According to the present invention, in an air suspension for preventing the spread of a new influenza virus by spraying an adsorbent to prevent the spread of the virus and further spraying in advance to normalize the air environment. An air suspension adsorbent that inactivates the influenza virus, a method for inactivating the influenza virus, and a method for producing the air suspension adsorbent for inactivating the influenza virus are provided.

Measurement results of plaque assays of Examples 1 and 2 and Comparative Example 1.

The air suspension adsorbent for inactivating the influenza virus in the air suspension according to the present invention, the method for inactivating the influenza virus, and the method for producing the air suspension adsorbent for inactivating the influenza virus are described below. To do. The air suspension adsorbent for inactivating the influenza virus in the air suspension according to the present invention, the method for inactivating the influenza virus, and the method for producing the air suspension adsorbent for inactivating the influenza virus are as follows: It is not limited to the embodiment.

In the spore growth inhibitory preparation previously disclosed by the present inventor in Japanese Patent No. 3491007, a growth inhibitory effect against Bacillus anthracis and Clostridium botulinum, which are spore-forming bacteria resistant to heat and chemical substances, was observed, and yeast It was also observed that the effect also affects mold fungi.

The present inventor has so far proposed sterilization of the food production environment, researched a method for removing airborne bacteria and falling bacteria, etc. We developed a food additive low-concentration alcohol preparation so that it can be sterilized in a manned environment. At the same time, as a result of investigating the movement of airborne bacteria during work, we confirmed that airborne bacteria gathered on the workers' heads at the start of operation. It was also found that if it was sprayed toward the surface, a net-breaking effect was obtained against airborne bacteria. In addition, the spore growth inhibitory preparation exhibits bactericidal effects against airborne and falling bacteria.

On the other hand, the effect of alcohol on viruses is known to require a concentration of 80-95% for poliovirus, echovirus, coxsackie virus, etc., but such high concentrations are sprayed in the air. Doing is dangerous. A low-concentration alcohol formulation was sought, although the spray method was adequate to capture the suspension, but without danger.

By the way, hemagglutinin HA on the influenza A virus membrane recognizes and binds to the sialic acid-containing complex sugar chain (receptor) of the host (human) cell membrane. In addition to HA, sialidase NA (neuraminidase) is present on the influenza A virus membrane, and the genes of HA and NA are likely to change, thereby acquiring new antigenicity and presuming that the immune surveillance mechanism is replaced. (Suzuki Yasuo, “Sugar Chain II”, Tokyo Kagaku Doujin, Virus Infection and Sugar Chain Biology, p.184-196).

Moreover, the sialo-glycan structure, which is a receptor to which influenza virus subtypes bind strongly, is commonly a lacto type I and type II (Non-patent Document 3), and the pocket for recognizing the receptor is very good. Vaccines are known to lose their potency due to subtype changes because conservatively, the amino acids in the antigenic determining region change rapidly.

No matter how the hemagglutinin and sialidase genes change, the site where hemagglutinin recognizes the sialic acid-containing complex sugar chain of the receptor is well conserved, so that hemagglutinin on the influenza virus membrane contains sialic acid in the host cell membrane The mechanism for recognizing and binding complex sugar chains establishes infection of influenza virus hosts. When infection is established, one virus will become 1 million in 24 hours, and the cells of the influenza host will be attacked 1 million times a day from infection of one influenza virus, The cells of the released host are killed (Non-patent Document 2). No matter how mutated, influenza virus does not change the mechanism of binding to the host cell membrane.

Since the mechanism by which hemagglutinin recognizes the sialic acid sugar chain is immobile, the present invention attempted to inactivate the influenza virus virus using this immobilization mechanism in intercellular recognition. It has also been found that direct contact of sialic acid with the influenza virus binds but is readily cleaved by sialidase on the influenza virus membrane. Viruses that float in the air do not float alone, and are thought to be attached to mold, bacteria, water droplets, and so on, so we researched air suspension adsorbents that comprehensively inactivate them.

(Embodiment 1)
(Air suspension adsorbent)
Hereinafter, an air suspension adsorbent according to an embodiment of the present invention will be described. In addition, this invention is not limited to the following embodiment.

(Material for air suspension adsorbent)
The air suspension adsorbent according to this embodiment includes ethanol, a surfactant (emulsifier), polylysine, glycine, and a food additive polymer. Moreover, the air suspension adsorbent according to this embodiment can further improve the inactivation effect of influenza virus by further adding a complex carbohydrate chain. You may denature ethanol by adding a fragrance | flavor.

As ethanol used for the air suspension adsorbent according to the present embodiment, 95 ° primary unmodified alcohol can be used.

Examples of the surfactant for the air suspension adsorbent according to the present embodiment include glycerin fatty acid ester, citric acid fatty acid monoglyceride, succinic acid fatty acid monoglyceride, diacetyltartaric acid fatty acid monoglyceride, diglycerin fatty acid ester, and sucrose fatty acid ester. Can be mentioned. The fatty acid may be either a saturated fatty acid or an unsaturated fatty acid.

As glycerin fatty acid ester, citric acid fatty acid monoglyceride, succinic acid fatty acid monoglyceride, diacetyl tartaric acid fatty acid monoglyceride, any one of C ₈ , C ₁₀ , C ₁₂ , and C ₁₈ carbon atoms can be used. The monoglyceride content is 80% or more, preferably 85% or more. Examples of the surfactant for the air suspension adsorbent according to the present embodiment include caprylic acid monoglyceride (Sunsoft (registered trademark) No. 700) and capric acid monoglyceride (Sunsoft (registered trademark) No. manufactured by Taiyo Kagaku Co., Ltd. 760), lauric acid monoglyceride (Sunsoft (registered trademark) No. 750), citric acid monostearate glyceride (Sunsoft (registered trademark) No. 621B), succinic acid monocaprylic acid glyceride (Sunsoft (registered trademark)) No. 682T), diacetyltartaric acid monostearate glyceride (Sunsoft (registered trademark) No. 641D), glycerin monocaprylate (Poem (registered trademark) M-100) from Riken Vitamin Co., Ltd., glycerin monocaprate (Poem (registered trademark)) Trademark) M-200), glycerin monolaurate (Poem (R) M-300), citric acid saturated fatty acid monoglyceride (Poem (TM) K-30), succinic acid fatty acid monoglyceride (Poem (TM) B-10) or the like can be used.

The air suspension adsorbent according to this embodiment may contain two or more kinds of glycerin fatty acid ester, citric acid fatty acid monoglyceride, succinic acid fatty acid monoglyceride, and diacetyltartaric acid fatty acid monoglyceride depending on the properties required. Also includes a number of carbon atoms of the fatty acid is different fatty acid esters of two or more, for example, it is also possible to include two types of glycerin fatty acid esters of C ₈ and C _10.

As the diglycerin fatty acid ester used for the surfactant of the air suspension adsorbent according to the present embodiment, a fatty acid having a carbon number of C ₁₄ and an HLB (Hydrophile-Lipophile Balance) value of 9.0 can be used. The fatty acid may be either a saturated fatty acid or an unsaturated fatty acid. As the surfactant for the air suspension adsorbent according to this embodiment, for example, diglycerin monomyristate (Poem (registered trademark) DM-100) manufactured by Riken Vitamin Co., Ltd. can be used.

As the sucrose fatty acid ester used for the surfactant of the air suspension adsorbent according to the present embodiment, any one of C ₁₂ , C ₁₆ , and C _{18 in} the fatty acid can be used. The fatty acid may be either a saturated fatty acid or an unsaturated fatty acid. Also includes a number of carbon atoms of the fatty acid is different fatty acid esters of two or more, for example, it is also possible to include two kinds of sucrose fatty acid esters of C ₁₂ and C _16. As the surfactant for the air suspension adsorbent according to this embodiment, 50 or more, preferably 70% or more of monoester is used. Examples of the surfactant for the air suspension adsorbent according to the present embodiment include DK Ester (registered trademark) F-160, F-110, S-L18A manufactured by Daiichi Kogyo Seiyaku Co., Ltd., Mitsubishi Chemical Foods Co., Ltd. Ryoto (registered trademark) Sugar Esters S-1570, O-1570, LWA-1570 and the like can be used.

As the polylysine used in the air suspension adsorbent according to this embodiment, ε-polylysine can be used, for example, a 25% solution manufactured by Nippon Chisso Corporation.

The glycine (glycol) used for the air suspension adsorbent according to the present embodiment can be used as a food additive.

As the complex carbohydrate sugar chain used in the air suspension adsorbent according to the present embodiment, N-acetylneuraminic acid and galactose having binding properties with influenza virus are used. N-acetylneuraminic acid and galactose may be added separately as the complex carbohydrate sugar chain used in the air suspension adsorbent according to this embodiment, or N-acetylneuraminic acid and galactose are chemically You may use what was couple | bonded. In addition, when an air suspension adsorbent is used for viruses and toxins other than influenza virus, N-acetylneuraminic acid and galactose are used instead of or in addition to the binding characteristics of inactivated viruses and toxins. -Acetylglucosamine, glucose, N-acetylgalactosamine and the like can be used.

As the food additive polymer used in the air suspension adsorbent according to this embodiment, any of polyacrylic acid sodium salt, sodium carboxymethyl cellulose, regenerated cellulose porous membrane, and polydextrose can be used.

Moreover, in the air suspension adsorbent according to the present embodiment, a known perfume may be added to alleviate the odor caused by the added drug and to change the unmodified alcohol to a modified alcohol. The fragrance | flavor can use what can be used as a food additive, without inhibiting inactivation of influenza virus with respect to each component of the above-mentioned air suspension adsorbent.

(Manufacturing method of air suspension adsorbent)
The air suspension adsorbent according to the present embodiment is first stirred while heating purified water to 46 ° C. or more and 67 ° C. or less, and the food additive polymer, glycine and polylysine are added and dissolved. Moreover, when adding complex carbohydrate sugar chain, it adds to this solution last. Separately, ethanol is added to a surfactant (if it is solidified, it is liquefied with a warm bath), and dissolved by stirring. This surfactant / ethanol solution is gradually added to the solution of the food additive polymer and the like, and dissolved by stirring to obtain a preparation.

The ethanol concentration should be within the range that can be handled as non-dangerous substances, and should be used and stored as a low-concentration ethanol preparation of 6.00 ^v / _v % or more and 50.00 ^v / _v % or less to maintain safety.

The concentration of monoglycerol fatty acid ester is preferably 0.02 ^v / _v % or more and 0.30 ^v / _v % or less, and the concentration of diglycerol fatty acid ester is preferably 0.042 ^v / _v % or more and 0.60 ^v / _v % or less. . The polylysine concentration is preferably 0.04 ^v / _v % or more and 0.60 ^v / _v % or less, and the glycine concentration is preferably 0.20 ^v / _v % or more and 3.00 ^v / _v % or less. The content of the food additive polymer is preferably 0.10 to 1.50%.

The concentration of the complex carbohydrate chain is preferably about 0.08 ^v / _v %. For example, N-acetylneuraminic acid is about 0.03 ^v / _v %, and galactose is about 0.05 ^v / _v %. Can be added. Perfume can be added at about 0.20 ^v / _v %.

The air suspension adsorbent according to the present embodiment has an excellent effect of inactivating influenza virus while being a low-concentration ethanol preparation due to mutual recognition of glycoconjugates and viruses. Moreover, as shown in the below-mentioned Example, the air suspension adsorbent according to the present embodiment is effective in inactivating influenza virus even when it does not contain complex carbohydrate sugar chains.

In addition to the influenza virus, the target cell recognition mechanism by neuraminic acid mutually recognizes bacterial cholera toxin, E. coli lytic toxin, tetanus toxin, botulinum toxin, clostridial toxin, Vibrio parahaemolytic thermolytic hemolysin, Shiga Shigella toxin, etc. This is considered to be effective against bacterial toxins adsorbed on neuraminic acid (Shinji Uchitaka, “Sugar II”, Sokkyo Chemical Dojin, Infection and Sugar Chains, p. 173 to 183). .

In addition, as shown in the examples described later, even in the case where complex carbohydrate sugar chains are not added to the air suspension adsorbent according to the present embodiment, there is a remarkable absence of influenza virus compared to ethanol at the same concentration. Shows the activation effect. Since there are no known reports of low-concentration ethanol preparations showing the inactivation effect of influenza virus, even in the case where complex carbohydrate sugar chains are not added in the air suspension adsorbent according to this embodiment, It is clear that it shows an excellent effect. The air suspension adsorbent according to the present embodiment can obtain further improved effects by adding complex carbohydrate sugar chains.

(Embodiment 2)
To inactivate viruses that have fallen on the floor, etc., or those that have adhered to walls or uneven surfaces, using an air suspension adsorbent, directly apply the air suspension adsorbent, soak it in a cloth, etc. However, in this embodiment, a method for spraying the air suspension adsorbent described in Embodiment 1 in the air will be described.

Air suspension particles of 3 microns or less containing microorganisms such as bacteria, molds and viruses are difficult to fall in still air. Air suspension particles flow with the movement of air and come into contact with people and food. When the air suspension adsorbent according to the present embodiment is sprayed, the air suspension particles come into contact with the air suspension adsorbent, and the air suspension adsorbent is absorbed to fall in weight. Bacteria and mold contained in the dropped air suspension particles are sterilized by the air suspension adsorbent, and the virus is inactivated.

In the air suspension adsorbent spraying method according to this embodiment, the air suspension adsorbent is sprayed as fine particles of 16 μm or less, preferably as fine particles of about 3 μm. By spraying the air suspension adsorbent as fine particles, the time for the air suspension adsorbent to float in the air becomes longer, and the contact efficiency with the air suspension particles can be increased. When the above-mentioned air suspension particles of 3 microns or less are targeted, spraying the air suspension adsorbent as fine particles of about 3 μm increases the contact efficiency with the air suspension particles. When sprayed with a general spray, the particle size of the air suspension adsorbent is increased and the residence time in the air is reduced, so that the contact efficiency with the air suspension particles is reduced. Further, by spraying the air suspension adsorbent as dry mist-like fine particles, clothes and objects of people in the sprayed space are not wetted.

In the spray method of the air suspension adsorbent according to the present embodiment, in preparation for the occurrence of new influenza, spraying for the purpose of preventing air infection and droplet infection, to prevent influenza virus infection from person to person Can do. In other words, the environment inside the aircraft can be purified by spraying to ensure the safety of passengers and prevent the entry of influenza viruses from abroad. In Japan, prevent propagation at home, the workplace, medical institutions, schools, and other places where many people gather, and always use it everywhere, such as when going out. You can protect yourself from the influenza virus and make a healthy maintenance until you get an influenza vaccine.

Since the air suspension adsorbent according to the present embodiment is sprayed as fine particles by the influenza virus inactivation method described above, the air suspension adsorbent is efficiently brought into contact with the air suspension particles, and thus is included in the air suspension particles. An excellent effect is that the influenza virus can be inactivated efficiently. In addition, the air suspension adsorbent according to the present embodiment can be sprayed using a device that generates dry mist that has been attracting attention in recent years as a countermeasure for the heat island phenomenon. Since it does not get wet, it can be used in various environments, both indoors and outdoors.

(Embodiment 3)
In the present embodiment, an example in which a two-component spray device is used for spraying the air suspension adsorbent described in the second embodiment will be described.

The aerial suspension adsorbent according to Embodiment 3 is obtained by purifying ethanol, a surfactant, polylysine, and glycine, a solution A obtained by solubilizing ethanol, a food additive polymer, a complex carbohydrate sugar chain, and a fragrance in purified water. Liquid B solubilized in water is respectively ejected by a two-fluid ejection device, and the two solutions collide and mix outside the nozzle and are released into the air. The released air suspension adsorbent mixture inactivates viruses contained in the air suspension.

In addition, the air suspension adsorbent according to Embodiment 1 can be sprayed as liquid A and an aqueous ethanol solution as liquid B more simply. Even when the air suspension adsorbent is sprayed using a two-component spray device, the air suspension adsorbent becomes fine particles of 16 μm or less, preferably about 3 μm, as described in the second embodiment. Spray like so.

According to the spray method of the air suspension adsorbent according to the present invention of the present embodiment, even when a two-component spray device is used, the air suspension adsorbent can inactivate influenza virus efficiently. it can.

The air suspension adsorbent described in Embodiment 1 was subjected to an inactivation test against influenza virus. In the inactivation test, as described below, evaluation was performed by an experiment in which the air suspension adsorbent and the influenza virus solution were directly mixed and a test in which the air suspension adsorbent and the influenza virus were contacted by spraying. In addition, it is clear that the air suspension adsorbent according to the present invention is not limited to the following and can be appropriately changed depending on various use conditions.

(Dilution test of air suspension adsorbent)
A test sample was prepared by serially diluting the air suspension adsorbent by two times, sensitized to the influenza virus solution, and the amount of proliferative influenza virus in the mixture with the influenza virus was evaluated by plaque assay. The details of the test are described below.

(Test sample)
As an example of the one-component air suspension adsorbent described in the second embodiment, the air suspension adsorbent having the composition shown in Table 1 is used as the air suspension adsorbent of Example 1 and Example 1. An air suspension adsorbent to which no acetylneuraminic acid was added was an ethanol solution having the same concentration as in Example 2, Examples 1 and 2, and Comparative Example 1 was used. Each solution was serially diluted two times to obtain a test sample. Here, 95 degree primary unmodified alcohol (Nippon Alcohol Sangyo Co., Ltd., traceable 95 primary) as ethanol, capric acid monoglyceride (Taiyo Chemical Co., Ltd., Sunsoft No. 760) as glycerin fatty acid ester, and diglycerin fatty acid ester Diglycerin monomyristate (RIKEN vitamin, Poem DM-100), ε-polylysine (Nippon Chisso Corporation, 25% solution) as polylysine, glycine as amino acid (Iwata Chemical Co., Ltd.), polydextrose as food additive polymer (Danisco Japan Co., Ltd., Lites (registered trademark) Ultra Powder), N-acetylneuraminic acid (Japan Food & Liquor Alliance Co., Ltd.) Mint essence Te (Koken perfume Co., Ltd., mint essence NO.64145) was used.

(Influenza virus solution)
For the influenza virus solution, A / Aichi / 2/68 strain (subtype: H3N2) was used, and the virus content was adjusted to 2.9 × 10 ⁷ PFU / ml.

(Sensitization to influenza virus solution)
The test samples of Examples 1 and 2 and Comparative Example 1 prepared as described above were mixed with 9 and influenza virus solution at a ratio of 1 and sensitized at room temperature for 1 minute. Moreover, it sensitized using the sterilized purified water as a control group.

(Evaluation by plaque assay)
The virus mixture after sensitization contains a culture medium for virus propagation (containing 0.2% sodium hydrogen carbonate, 3 mM L-glutamine, 0.2% albumin, 10,000 IU / ml penicillin and 10,000 μg titer / ml streptomycin. Dilute serially 10 times with MEM (Minimum Essential Medium) medium. The medium was removed from MDCK (Madin-Darby canine kidney) cells cultured in MEM medium containing fetal calf serum so as to form a monolayer on a 6-well plate, and washed once with PBS (−). Influenza virus was adsorbed by adding 0.1 ml of a diluted mixture per MDCK cell per well and culturing for 60 minutes. Thereafter, the mixed solution is removed, and 2-3 ml of primary layered agar medium (virus culture medium containing 0.1% glucose, 2.5 μg / ml trypsin and 0.8% agarose) is added to solidify the agarose. The cells were cultured at 34 ° C. in a 5% carbon dioxide atmosphere for 2 days. Furthermore, 2 ml of a secondary multi-layered agar medium (1% agarose gel, 0.01% neutral red-containing virus growth medium) was added and solidified, and cultured overnight at 34 ° C. in a 5% carbon dioxide atmosphere. The number of plaques formed in the wells to which the serially diluted mixture was added was counted.

The measured number of plaques is shown in FIG. Moreover, LRV (Log Reduction Value) with respect to a control group was calculated about each test sample, and effectiveness was evaluated. Here, assuming that a test sample having an LRV of 2 or more is effective, Example 1 was effective up to 4-fold dilution, and Example 2 was effective up to 2-fold dilution. On the other hand, Comparative Example 1 consisting of ethanol with the same concentration as in Example 1 and Example 2 did not show the effect on influenza virus even in the stock solution.

From the above results, the air suspension adsorbent according to the present invention of Example 1 is effective for inactivating influenza virus, and is effective for inactivating influenza virus even at an ethanol concentration of only 7.4%. I understand that there is. In the air suspension adsorbent according to the present invention of Example 1, N-acetylneuraminic acid and galactose were added. From this result, N-acetylneuraminic acid and galactose were chemically bonded. Even if a sugar chain is used, it is considered to be effective in inactivating influenza virus. In addition, it can be seen that the air suspension adsorbent according to the present invention of Example 2 that does not contain N-acetylneuraminic acid is also effective in inactivating influenza viruses although it is weaker than Example 1. Therefore, the air suspension adsorbent according to the present invention has an excellent effect of enabling inactivation of influenza virus while ethanol is at a low concentration. Moreover, since the air suspension adsorbent according to the present invention of Example 2 is composed only of substances recognized as food additives, there is little danger to the human body and the environment.

(Influenza virus inactivation effect by spraying air suspension adsorbent)
In the above-mentioned examples, the inactivation effect under the condition in which the air suspension adsorbent and influenza virus solution were directly mixed was verified, but in the examples described below, the air suspension adsorbent and influenza virus were used. The inactivation effect of the air suspension adsorbent under the more practical conditions sprayed with the liquid was verified.

(Test sample)
What sprayed the stock solution of the air suspension adsorbent of Example 1 was set to Example 3, and what sprayed the ethanol solution of Comparative Example 1 was set to Comparative Example 2. 1 ml each was used for spraying.

(Influenza virus solution)
The above influenza virus solution was prepared so that the virus content was 2.5 × 10 ⁸ PFU / ml. The spray amount was 1 ml.

In a plastic box having a width of 800 mm × depth 400 mm × height 400 mm, the air suspension adsorbent and the influenza virus solution of Example 3 were put in a Jackson-type laryngeal anesthesia machine, respectively. The air suspension adsorbent and the influenza virus solution were alternately sprayed several times in this order, and the air suspension adsorbent and the influenza virus solution were contacted and mixed in the air. Six plastic petri dishes with the lid open were placed on the floor of the plastic box, and the influenza virus that fell was collected. When 10 minutes had elapsed after the completion of spraying, the plastic petri dish was covered and the plastic petri dish was collected from the plastic box.

To the collected plastic petri dish, 5 ml of a virus growth medium was added to obtain a virus mixture. The same operation was performed for the ethanol solution of Comparative Example 2, and each virus mixture was serially diluted, and the inactivation effect against influenza virus was evaluated by the plaque assay described above.

In Example 3 and Comparative Example 2, the virus content detected for three petri dishes placed on the floor is shown in Table 2.

In the results shown in Table 2, since the amount of influenza virus detected in Example 3 was reduced by 10 ² PFU / ml compared to Comparative Example 2, influenza virus was inactivated by spraying the air suspension adsorbent of Example 3. You can see that This example is a result of simple spraying of the air suspension adsorbent in the laboratory, but by using the spray device as described in

Embodiments

2 and 3, the air suspension adsorbent is finer. It is possible to inactivate the influenza virus more effectively by improving the spray efficiency and the contact efficiency against the influenza virus by spraying as simple particles.

As described above, according to the spray of the air suspension adsorbent according to the present invention of Example 3, there is an excellent effect capable of inactivating influenza virus that is difficult to inactivate only with an alcohol solution.

Claims

An air suspension adsorbent that inactivates influenza virus, including ethanol, surfactant, polylysine, glycine, and food additive polymer.
The air suspension adsorbent for inactivating influenza virus according to claim 1, wherein water is used as a solvent for the air suspension adsorbent.
The surfactant is selected from any one of glycerin fatty acid ester, citric acid fatty acid monoglyceride, succinic acid fatty acid monoglyceride, diacetyltartaric acid fatty acid monoglyceride, diglycerin fatty acid ester, and sucrose fatty acid ester, or a combination of two or more. An air suspension adsorbent that inactivates the influenza virus according to 1.
The influenza virus according to claim 3, wherein the glycerin fatty acid ester, the citric acid fatty acid monoglyceride, the succinic acid fatty acid monoglyceride, and the diacetyltartaric acid fatty acid monoglyceride are selected from any of 8, 10, 12, and 18 carbon atoms. Inactive air suspension adsorbent.
The air suspension adsorbent for inactivating influenza virus according to claim 3, wherein the diglycerin fatty acid ester has 14 fatty acids.
The air suspension adsorbent for inactivating the influenza virus according to claim 3, wherein the sucrose fatty acid ester is selected from any of 12, 16, and 18 carbon atoms of fatty acid.
The air suspension adsorbent for inactivating influenza virus according to claim 6, wherein the food additive polymer is selected from any of polyacrylic acid sodium salt, carboxymethylcellulose sodium, regenerated cellulose porous membrane, and polydextrose. .
The air suspension adsorbent for inactivating the influenza virus according to claim 7, wherein the air suspension adsorbent further comprises a complex carbohydrate chain.
9. The air suspension adsorbent for inactivating influenza virus according to claim 8, wherein the glycoconjugate sugar chains are N-acetylneuraminic acid and galactose.
The air suspension adsorbent for inactivating the influenza virus according to claim 9, wherein the air suspension adsorbent further contains a fragrance.
A method of inactivating influenza virus by spraying an air suspension adsorbent containing ethanol, a surfactant, polylysine, glycine, and a food additive polymer.
Spraying the first solution containing the ethanol and the food additive polymer and the second solution containing the ethanol, the surfactant, the polylysine and the glycine, respectively;
The method for inactivating influenza virus according to claim 11, wherein the first solution and the second solution are mixed in the air.
The method for inactivating influenza virus according to claim 11, wherein water is used as a solvent for the air suspension adsorbent.
The surfactant is selected from any one of glycerin fatty acid ester, citric acid fatty acid monoglyceride, succinic acid fatty acid monoglyceride, diacetyltartaric acid fatty acid monoglyceride, diglycerin fatty acid ester, and sucrose fatty acid ester, or a combination of two or more. 11. A method for inactivating the influenza virus according to 11.
The influenza virus according to claim 14, wherein the glycerin fatty acid ester, the citric acid fatty acid monoglyceride, the succinic acid fatty acid monoglyceride, and the diacetyltartaric acid fatty acid monoglyceride are selected from any of 8, 10, 12, and 18 carbon atoms. How to inactivate.
The method for inactivating influenza virus according to claim 14, wherein the diglycerin fatty acid ester has 14 fatty acids.
The method for inactivating the influenza virus according to claim 16, wherein the sucrose fatty acid ester is selected from any of 12, 16, and 18 carbon atoms of fatty acid.
The method for inactivating an influenza virus according to claim 17, wherein the food additive polymer is selected from any of polyacrylic acid sodium salt, carboxymethyl cellulose sodium, regenerated cellulose porous membrane, and polydextrose.
The method for inactivating an influenza virus according to claim 18, wherein the air suspension adsorbent further comprises a complex carbohydrate sugar chain.
The method for inactivating an influenza virus according to claim 19, wherein the glycoconjugate sugar chains are N-acetylneuraminic acid and galactose.
The method for inactivating an influenza virus according to claim 20, wherein the air suspension adsorbent further comprises a fragrance.
Water is heated to 46 ° C. or more and 67 ° C. or less, and a food additive polymer, glycine and polylysine are added and dissolved to prepare a first solution,
The ethanol is added to the surfactant and dissolved to prepare a second solution,
A method for producing an air suspension adsorbent that inactivates influenza virus, wherein the second solution is prepared by dissolving the second solution in the first solution.
The method for producing an air suspension adsorbent for inactivating an influenza virus according to claim 22, wherein a complex carbohydrate sugar chain is further added to the first solution.
The surfactant is selected from any one of glycerin fatty acid ester, citric acid fatty acid monoglyceride, succinic acid fatty acid monoglyceride, diacetyltartaric acid fatty acid monoglyceride, diglycerin fatty acid ester, and sucrose fatty acid ester, or a combination of two or more. A method for producing an air suspension adsorbent that inactivates the influenza virus according to 22.
23. The influenza virus according to claim 22, wherein the glycerin fatty acid ester, the citric acid fatty acid monoglyceride, the succinic acid fatty acid monoglyceride, and the diacetyltartaric acid fatty acid monoglyceride are selected from any of 8, 10, 12, and 18 carbon atoms. A method for producing an inactive air suspension adsorbent.
The method for producing an air suspension adsorbent for inactivating the influenza virus according to claim 22, wherein the diglycerin fatty acid ester has 14 fatty acids.
23. The method for producing an air suspension adsorbent for inactivating influenza virus according to claim 22, wherein the sucrose fatty acid ester is selected from any of 12, 16, and 18 carbon atoms of fatty acid.
23. The air suspension adsorbent for inactivating influenza virus according to claim 22, wherein the food additive polymer is selected from any of sodium polyacrylate, sodium carboxymethylcellulose, regenerated cellulose porous membrane, and polydextrose. Manufacturing method.
The method for producing an air suspension adsorbent for inactivating influenza virus according to claim 23, wherein the complex carbohydrate sugar chains are N-acetylneuraminic acid and galactose.
The manufacturing method of the air suspension adsorbent which inactivates the influenza virus of Claim 22 in which the said air suspension adsorbent further contains a fragrance | flavor.