JP5618218B2 - Bactericidal water, antiviral agents, cleaning water for sterilization in fresh food processing systems and insecticides for poultry farming - Google Patents

Description

  The present invention relates to a sterilized water, antiviral agent, and fresh food processing system using a calcined calcium powder derived from a shell that exhibits bactericidal and viral inactivation against various viruses that cause food poisoning and pathogens of infectious diseases. The present invention relates to cleaning water for sterilization and insecticide for poultry farming.

  Conventionally, about 400,000 to 500,000 tons of scallop shells are discharged annually, and some of them are used for food additives, fertilizers, etc., but most are still discarded as industrial waste. New technology development for widespread use is expected.

  In recent years, many food poisoning caused by Gram-positive bacteria (such as Staphylococcus aureus) and Gram-negative bacteria (Pathogenic Escherichia coli, Salmonella, Campylobacter, etc.) has occurred. To prevent the occurrence of food poisoning, More hygiene measures are required in poultry processing plants.

  Therefore, livestock that are meat animals based on the slaughterhouse law (in Japan, only five kinds of livestock such as cattle, horses, pigs, sheep and goats, and deer and frogs are not covered by the law) Washed in a chiller tank filled with a weakly acidic hypochlorous acid water (hereinafter also referred to as “chiller water”), which is a sterilizing wash water to which an appropriate amount of dilute hydrochloric acid for adjusting pH is added. After sterilization of various bacteria related to food poisoning, the slaughter inspector who has the license of a meat hygiene inspection laboratory or a veterinarian belonging to the health center undergoes an inspection (biological examination) such as illness.

  However, the presence of organic substances in hypochlorous acid water decreases the sterilizing power, and further contains chlorine compounds, so it generates an unpleasant odor (chlorine odor), and the generation of chlorine gas contributes to human health. There was a problem of harm. In addition, since chlorine compounds are corrosive due to their oxidizing power, there is also a problem that corrosion occurs on facilities and machinery.

  As a result of earnest research, the inventors of the present application have found that a shell-calcined calcium powder obtained by pulverizing and firing shells such as scallops and a shell-calcined calcium mixture containing this shell-calcined calcium powder have a bactericidal effect and antibacterial effect. Acquired various viewpoints that have effects, do not irritate the human body, etc., and do not generate heat, and calcined shell calcium powder such as scallops discharged as industrial waste, shells An antiviral material using a calcined calcium mixture was developed (for example, Patent Document 1).

JP 2012-62257 A

  However, the antiviral material of Patent Document 1 can be expected to have a bactericidal effect only up to about 5 to 10 times as a dilution factor of the stock solution. Therefore, for example, when used as chiller water in a fresh food processing plant such as vegetables, livestock, and seafood, a large amount of antiviral material must be used, and the use is limited from the cost aspect. was there.

  In addition, highly pathogenic avian influenza virus (HPAIV), foot-and-mouth disease, parvovirus (goose parvovirus (GPV), porcine parvovirus (PPV), mouse parvovirus (MPV) having the highest level of physicochemical resistance among viruses) In order to prevent these infectious diseases without using a vaccine, the enhancement of biosecurity is important, and the development of antiviral agents that serve as chemical biosecurity enhancement materials is strongly desired.

  In particular, GPV is a highly contagious disease similar to the French gamoparvovirus (MDPV) as a virus having the strongest level of resistance. It is fatal at less than one month of age and causes tremendous damage in virgin areas. . In addition, depending on the age at which chicks are infected, it takes an acute, subacute, or chronic form, and when chicks younger than 1 week are infected, they see a 100% mortality rate. It is one. GPV and MDPV are physicochemically stable viruses that can survive long in the field, are resistant to disinfectants, and once contaminated, it is impossible to make the farm virus-free .

  Furthermore, since GPV is highly resistant to heat and disinfectants, it takes 30 minutes to reduce the titer to less than the detection limit even after sensitizing for 15 minutes at 80 ° C. In addition, the disinfectant sodium hypochlorite is effective, but sterilization time is long because it requires sensitization at 4000 ppm for 30 minutes to make the titer against GPV less than 1/1000. In addition, a very strong chlorine odor was generated and there was a problem in handling.

  Therefore, the present invention has been made in view of the above-mentioned problems, and it leads to environmental conservation by recycling shells, and also has a bactericidal effect and virus inactivity applicable to various highly efficient and convenient applications. An object of the present invention is to provide a sterilizing water, an antiviral agent, a sterilizing washing water in a fresh food processing system, and a poultry insecticide.

In order to achieve the above-mentioned object, the sterilizing water according to claim 1 of the present invention comprises a shell calcined calcium having an average particle diameter of 25 nm to 10 μm in a range of 0.01 to 30% by weight, of sodium carbonate or potassium carbonate. In the range of 0.01 to 30% by weight of carbonates containing at least one or more , 0 to organic acid salts containing at least one or more of sodium citrate, potassium citrate, sodium malate, sodium ascorbate or sodium acetate .01～30 wt% range, and wherein the dissolved natural porous clay mineral consisting mainly of montmorillonite and vermiculite in pure water at a range of 0.01 to 30 wt%.

The sterilizing water according to claim 2 is characterized in that the sterilizing water according to claim 1 further contains sodium hypochlorite or chlorine dioxide in the range of 0.01 to 30% by weight as chlorines .

The sterilized water according to claim 3 is the sterilized water according to claim 1 or 2, further comprising at least one selected from metroses, casein, gelatin, starch, cellulose, dextrin, and hyaluronic acid as a thickening agent. It is characterized by containing in the range of 0.01 to 30% by weight .

The antiviral agent according to claim 4 is the sterilized water according to claim 1 or 2, or a drug containing the sterilized water .

The cleaning water for sterilization in the fresh food processing system according to claim 5 is the sterilizing water according to claim 1 or 2 or an aqueous solution containing the sterilizing water .

The insecticide for poultry farming according to claim 6 is the sterilized water according to claim 3 or a medicine containing the sterilized water .

  According to the present invention, it contains shell-calcined calcium obtained by firing shells treated as industrial waste, and carbonates, organic acid salts and natural porous clay minerals that are harmless to the human body. It is possible to provide an excellent antiviral agent showing antiviral activity against AIV, GPV and the like, as well as sterilizing water having high sterilizing effect against causative bacteria causing food poisoning, as well as environmental conservation by recycling.

  Further, since the sterilizing effect can be maintained even when the dilution factor is 100 times or more, it can be introduced at a low cost even in a large-scale factory. Furthermore, even when chlorine is included to improve the bactericidal effect, the causative bacteria of food poisoning in the presence of organic substances can be efficiently sterilized even if the content is small, so the effect of preventing the generation of chlorine odor as much as possible Can be played.

  In addition, by using the sterilized water of the present invention as cleaning water for sterilization in fresh food processing systems for livestock meat, seafood, vegetables, fruits, etc., the bacteria causing food poisoning in the fresh food processing plant can be effectively sterilized. can do.

  Furthermore, since the sterilizing water of the present invention contains a thickener or polysaccharide as a thickening agent, it exhibits a repellent effect against pests such as vaccinia mites that live in poultry farms, and safety that exerts a slaughtering (suffocation) effect High pesticides for poultry farming can be provided.

It is a table | surface which shows the test result of the bactericidal effect with respect to colon_bacillus | E._coli and Staphylococcus aureus using the mixing | blending ratio of the sterilizing water based on this invention, and this sterilizing water. It is a table | surface which shows the test result of the bactericidal effect with respect to the mixing ratio of the sterilizing water based on this invention, and Campylobacter using this sterilizing water. It is a table | surface which shows the living microbe used for the sterilization test of the sterilizing water based on this invention, and the living microbe measurement result of coliform group. It is a table | surface which shows the test result of the bactericidal effect with respect to the mixing | blending ratio of the sterilizing water based on this invention, and the living microbe and coliform group shown in FIG. 3 which used this sterilizing water. It is a table | surface which shows the test result of the bactericidal effect with respect to the mixing | blending ratio of the sterilizing water based on this invention, and the living microbe and coliform group shown in FIG. 3 which used this sterilizing water. It is a table | surface which shows the test result of the bactericidal effect with respect to the mixing | blending ratio of the sterilizing water based on this invention, and the living microbe and coliform group shown in FIG. 3 which used this sterilizing water. It is a table | surface which shows the repelling and insecticidal result with respect to the mite which used the germicidal water concerning this invention, and the germicidal water using this germicidal water. It is a table | surface which shows the test result of the metal corrosion experiment by the sterilizing water based on this invention. (A) is a mixing | blending table | surface which shows the compounding ratio at the time of applying the sterilized water which concerns on this invention to a fresh food processing system, (b) is the washing | cleaning for sterilization of a fresh food processing system using the sterilizing water which concerns on this invention It is a table | surface which shows the sterilization result when applied as water. (A) is a mixing | blending table | surface which shows the mixing | blending ratio of the sterilizing water based on this invention, (b) is a table | surface which shows the virus suppression index | exponent with respect to AIV and GPV using the sterilizing water which concerns on this invention.

  Hereinafter, embodiments for carrying out the present invention will be described in detail with reference to the accompanying drawings. It should be noted that the present invention is not limited by this embodiment, and all other forms, examples, operation techniques, etc. that can be implemented by those skilled in the art based on this form are included in the scope of the present invention. .

  The sterilized water of the present invention has a mean particle size of 25 nm to 10 μm in the range of 0.01 to 30% by weight of the calcined shell calcium powder, the carbonate in the range of 0.01 to 30% by weight, and the organic acid salt in the range of 0.1 to 30% by weight. Highly versatile alkaline aqueous solution having a sterilizing, antibacterial and deodorizing function (pH of about 9 to 13) obtained by dissolving 0.01 to 30% by weight of natural porous clay mineral in pure water in the range of 01 to 20% ).

  As pure water, water (RO water, deionized water, distilled water) from which impurities are removed using a reverse osmosis membrane, an electric regenerative ion exchange device, a distiller, or the like, medical purified water, or the like can be used.

  Shell-calcined calcium powder (calcined CaO) is a shell of shellfish (scallops, clams, swordfish, clams, oysters, scallops, etc., preferably scallops with high bactericidal and virus inactivation) Is a calcium oxide powder obtained by firing at a predetermined temperature and passing through an appropriate pulverization step, and is contained in the range of 0.01 to 30% by weight depending on the application.

  As a method for producing shell-calcined calcium, it is important to first remove the edible portion of the shell, remove the deposits attached to the shell, and carefully wash the salt with fresh water. Next, using a pulverizer such as a crusher or a ball mill, a ground pulverized product of about 20 to 30 mm is prepared. Then, this ground pulverized product is baked in a baking pot in the range of about 900 to 1100 ° C. for 1 to 5 hours and cooled for about 12 to 24 hours. Thereafter, a shell-fired calcium powder having a particle size of about 10 to 15 μm is prepared by a dry bead mill or a pulverizer.

  Moreover, as a method for making the shell-calcined calcium powder having the above particle size finer (average particle size: 500 nm to 10 μm), the particles were accelerated by high-pressure gas energy, and a concentric rotating vortex forming machine using a high-pressure jet stream was used. Finer nanoparticles can be obtained by causing collision between particles by the nozzle. Furthermore, as a method for further refined (calculated average particle size of 20 to 500 nm) of shell calcined calcium finely processed by the swirl vortex generator using the high-pressure jet stream, it is generated in a high-frequency heat field using high-frequency thermal plasma having a particle size. The calcium powder supplied to the thermal plasma (center temperature is about 10,000 ° C., outside the center is about 6000 to 8000 ° C.) instantly evaporates, and then undergoes an agglomeration process to produce ultrafine shell calcined calcium powder. Can be obtained.

  In addition, although a high pressure gas energy system or a high frequency thermal plasma system can be arbitrarily selected according to a use, you may obtain shell calcination calcium powder using both systems depending on a use purpose.

  Further, the sterilized water of the present invention is a carbonate / calcium salt in order to improve the dissolution / dispersion function, antibacterial, antiviral, protein and fat decomposition function, deodorizing effect, etc. of the shell calcined calcium powder and to stabilize the aqueous solution. Contains organic acid salts and natural porous clay minerals.

  As carbonates, sodium carbonate, potassium carbonate, etc. are arbitrarily selected and used according to a use, and it contains in 0.01-30 weight%.

  As the organic acid salt, at least one or more kinds selected from sodium citrate, potassium citrate, sodium malate, sodium ascorbate, sodium acetate, etc. depending on the use are used, and 0.01 to 30% by weight In the range of.

  The natural porous clay mineral is a clay mineral mainly composed of montmorillonite and vermiculite such as ULTRA-ION (manufactured by Organic Science Co., Ltd.) marketed as an improved soil for agriculture. It is contained in the range of 01 to 30% by weight.

  In addition, the sterilizing water of the present invention has a viscous property such as thickeners and polysaccharides in order to retain the solution at the time of spraying by giving it a viscous property or to give an effect of suffocation and repelling of pests to be sprayed. An agent can also be added.

  As the thickener, at least one or more kinds of thickeners and gelling agents used as food additives, such as metroise, casein, gelatin, starch, cellulose, dextrin, and hyaluronic acid, can be arbitrarily selected. And contained in the range of 0.01 to 30% by weight depending on the application. The thickening agent is effective for imparting antibacterial properties to foods and measures against plant pests when added to impart consistency to sterilized water.

  Furthermore, the sterilizing water of the present invention uses at least one or more kinds of chlorines such as sodium hypochlorite and chlorine dioxide for the purpose of further improving the sterilizing effect. It can also be contained in the range of 0.01 to 30% by weight.

  Sodium hypochlorite is essentially alkaline, but its bactericidal effect is weak in the alkaline region. Compared to the acid side aqueous solution and neutral aqueous solution, the bactericidal effect is stronger, so in recent years, sodium hypochlorite has been added to dilute hydrochloric acid. The added hypochlorous acid water is used, but this sterilizing water can be expected to have a bactericidal and antiviral effect that is higher than that of hypochlorous acid water even though it is alkaline and contains a small amount of chlorine. , Can significantly reduce the unique chlorine odor.

  Next, the manufacturing method of the sterilizing water of this invention is demonstrated. First, natural porous clay minerals, carbonates, and organic acid salts are mixed and stirred in order in pure water, and the necessary amount of the calcined shell shell calcined calcium powder is mixed and charged, and uniformly dissolved and dispersed using a stirrer. Can be obtained.

  A wet emulsifying disperser is used as a means for improving the dissolution and dispersion of the calcined shell calcium powder. This disperser gives a powerful impact in megahertz units to the aqueous solution by a specially shaped rotor that rotates at high speed and a stator that meshes with it, and it achieves the emulsification and dispersion effect equivalent to that of a high-pressure homogenizer even with a rotary type. It is possible to further stabilize the concentrated mixed aqueous solution. For selection of the stirrer of the concentrated mixed aqueous solution, a wet bead mill, a ball mill, a jet mill, a mechanical device using collision energy of ultrahigh pressure water, or the like can be selected.

  Moreover, according to a use, a thickener and chlorine can also be stirred and mixed according to a required mixing | blending design ratio. Furthermore, in view of the purpose of use, work efficiency during the manufacturing process, and the like, the stirring-in order of the contents can be appropriately changed.

  The sterilized water of the present invention includes a shell-calcined calcium powder obtained by firing a recycled shell, a dissolution / dispersion function of the shell-calcined calcium powder, an antibacterial function, an antiviral function, a protein and fat decomposition function, a deodorizing effect, etc. In order to improve the temperature and stabilize the aqueous solution, it is an aqueous solution produced by containing carbonates, organic acid salts and natural porous clay minerals in pure water.

  Therefore, it has a bactericidal effect and biofilm dissolution / removal effect while being in an alkaline region, and further increases the degree of freedom of the dilution factor, thereby expanding the range of uses from household use to industrial use, as well as the causative bacteria of food poisoning Virus inactivation can be imparted to various viruses that are pathogens for sterilization and infectious diseases.

  In addition, even when used containing chlorines, it has a bactericidal effect in the presence of organic matter despite being alkaline, and further suppresses the generation of chlorine gas since the chlorine content is very small. The effect of greatly reducing the chlorine odor and preventing the corrosion of facilities and equipment can be achieved.

  In addition, since the liquid is alkaline, it is possible to correct acidic soil and to expect repellent effects and insecticidal effects (choking death or drowning) against pests and small organisms in plant cultivation. Furthermore, minerals contained in natural porous clay minerals serve as a nutrient supply source for plants.

  The sterilizing water of the present invention is a sterilizing cleaning water for a sterilizing agent, an antibacterial agent, a cleaning agent, an antiviral agent, an insecticide, or a fresh food product by adjusting the mixing ratio of the above-mentioned compound according to its use. In addition, it can be used for ice for preserving fresh food products, etc., and can be used for the above-mentioned purposes by containing it in another solvent capable of maintaining the effect of the sterilizing water and adjusting the concentration appropriately.

  Examples according to the present invention will be described below. It should be noted that the present invention is not limited to the embodiments described in these examples, and any design changes in light of the gist of the preceding and following descriptions are included in the technical scope of the present invention. .

  First, the sterilizing water according to the present invention was prepared by blending an appropriate amount of an appropriate content in accordance with the test content of each Example shown below along the above production method. The blending ratio of the produced sterilizing water will be described in each example.

[Example 1]
<Purpose of test>
In order to confirm the bactericidal effect of the sterilizing water according to the present invention, a comparative test of the bactericidal effect against Escherichia coli and Staphylococcus aureus that cause food poisoning was performed.
<Adjustment of sterilizing water>
The mixing ratio of the sterilizing water used in this example is as shown in FIG.
<Test strain>
As test strains, E. coli (Etaherichia coli NBRC 3972) and Staphylococcus aureus subsp. Aureus NBRC 12732 were used.
<Bacteria count culture medium and culture conditions>
The 35 ° C. ± 1 ° C. Each test strain in nutrient agar (Eiken Chemical Co., Ltd.), after incubation for 18-24 hours, purified water (Staphylococcus aureus saline) were suspended in the number of bacteria 10 ⁷ It adjusted so that it might become -10 < ⁸ > / ml, and it was set as the test microbe liquid. Further, each strain was cultured by a pour plate culture method (35 ° C. ± 1 ° C., 2 days) using SCDLP agar medium (Nippon Pharmaceutical Co., Ltd.).
<Test operation>
The sterilized water of the present invention is diluted 10, 40, and 50 times using purified medical water, and 0.1 ml of the test bacterial solution is inoculated into 10 ml of each diluted solution to prepare sterilized water at 20 ° C. ± 1 ° C. saved. After 30 minutes, the preserved sterilized water was immediately diluted 10-fold with SCDLP agar medium (Nippon Pharmaceutical Co., Ltd.) and cultured by pour plate culture (35 ° C ± 1 ° C, 2 days). The number of bacteria was measured using a culture medium for measuring the number of bacteria.

<Test results>
As shown in FIG. 1, E. coli was not detected at any of 10-fold, 40-fold and 50-fold dilutions. Neither 10-fold nor 40-fold dilution was detected for Staphylococcus aureus, and it was confirmed that Escherichia coli and Staphylococcus aureus that cause food poisoning were sterilized by this sterilizing water. In addition, even after 6 months, it was confirmed that the sterilized water of the present invention maintained the bactericidal effect against Escherichia coli and Staphylococcus aureus.

[Example 2]
<Purpose of test>
In order to confirm the sterilizing effect of the sterilizing water according to the present invention, a comparative test of the sterilizing effect against Campylobacter, which is a causative agent of food poisoning that is difficult to reduce even with sodium hypochlorite, was performed.
<Adjustment of sterilizing water>
The mixing ratio of the sterilizing water used in this example is as shown in FIG.
<Test strain>
Campylobacter (Campylobacter jejuni subsp. Jejuni ATCC 33560) was used as a test strain.
<Bacteria count culture medium and culture conditions>
Each test strain was microaerobically cultured for 5 days in a plate smear culture method (35 ° C ± 1 ° C) using equine defibrinated blood (Blood Agar Base No.2 (OXOID)) and then suspended in physiological saline. And adjusted to a bacterial count of 10 ⁷ to 10 ⁸ / ml to obtain a test bacterial solution.
<Test operation>
The sterilized water of the present invention is diluted 30-, 50-, and 70-fold with medical purified water, and 0.1 ml of the test bacterial solution is inoculated into 10 ml of each diluted solution to produce sterilized water, saved. After 30 minutes, the preserved sterilized water was immediately diluted 10-fold with SCDLP agar medium (Nippon Pharmaceutical Co., Ltd.) and cultured by pour plate culture (35 ° C ± 1 ° C, 2 days). The number of bacteria was measured using a culture medium for measuring the number of bacteria.

<Test results>
As shown in FIG. 2, Campylobacter was not detected at 30-fold, 50-fold or 70-fold dilution, and it was confirmed that Campylobacter which is a causative agent of food poisoning was sterilized by the sterilized water of the present invention.

  Next, in Examples 3 to 5, a food stamp (registered trademark) “Nissui” (standard agar, X-GAL agar, hereinafter simply referred to as “stamp medium”) manufactured by Nissui Pharmaceutical Co., Ltd. is used. A comparative test was carried out in which the number of bacteria (colony) of the control specimen components was counted.

  The test bacterial solution used in Examples 3 to 5 was prepared by mixing 100 g of commercially available chicken thighs and breasts and 200 ml of medical purified water in a container and storing them in a Franky at 37 ° C. for 48 hours. This is a solution obtained by culturing adhering bacteria (live bacteria, E. coli) and removing solid meat pieces.

In addition, in order to confirm the survival of viable bacteria and coliform bacteria in the prepared test bacterial solution, the number of bacteria was measured by the following method. First, two thin fabrics of clean gauze are cut into 4 cm square (16 cm ² ), and two sheets of gauze for viable bacteria and coliform bacteria are prepared as contact surfaces for the stamp medium. A plastic 150 mm x 100 mm x 5 mm plate Two pieces of cut gauze were placed on the surface of the slab after a commercially available Saran Wrap (registered trademark) was laid. Next, the total amount of the plastic plate, Saran Wrap (registered trademark), and two gauze sheets was adjusted to a zero base point using a measuring instrument and reset, and each test bacterial solution and the diluted sterilized water were taken out with a pipette. Then, 1 g of each solution was uniformly dropped into two sheets of gauze and wetted. Next, two wet gauze and the surface of the stamp medium are brought into contact with each other. After culturing at 37 ° C. for 48 hours with a Franky, the control specimen components are transferred to the surface of the medium using the stamp medium. The number of bacteria (colonies) that grew was counted.

  As shown in FIG. 3, the bacterial solution obtained by diluting the test bacterial solution 10-fold is “10-A”, the bacterial solution obtained by diluting the test bacterial solution 20-fold is “20-A”, and the test bacterial solution diluted 30-fold. The liquid was expressed as “30-A”, and the bacterial liquid obtained by diluting the test bacterial liquid 40 times was expressed as “40-A”. Further, a bacterial solution was prepared by diluting 1 ml of the diluted bacterial solution in 9 ml of medical purified water, and the 10-fold diluted bacterial solution obtained by diluting the “10-A” diluted bacterial solution 10-fold was designated as “10-B”, “20- A "diluted solution 10 times diluted 200 times diluted bacterial solution" 20-B "," 30-A "diluted bacterial solution 10 times diluted 300 times diluted bacterial solution" 30-B "," 40-A " The 400-fold diluted bacterial solution obtained by diluting the diluted bacterial solution 10 times was expressed as “40-B”. Furthermore, 1 ml of purified water was moistened in the gauze, and the control which was tested according to the above test procedure was expressed as “0-C”. As shown in the figure, the survival of viable bacteria and coliforms was confirmed in each bacterial solution.

[Example 3]
<Purpose of test>
A comparative test was conducted to confirm the presence or absence of the bactericidal effect when chlorines were added to the sterilized water of the present invention.
<Adjustment of sterilizing water>
The mixing ratio of the sterilizing water used in this example is as shown in FIG. Moreover, the chlorine water which added sodium hypochlorite 0.025 weight% (30 ppm) to RO water was produced as "4-1" as the conventional chlorine water for disinfection used as a comparison control.
In addition, 0.025 weight% (30 ppm) of sodium hypochlorite is a general concentration area | region currently utilized when sterilizing foodstuffs.
<Adjustment of test bacterial solution>
The prepared “10-B” (100-fold diluted test bacterial solution) was used as the test bacterial solution.
<Test operation>
1 ml of the test bacterial solution “10-B” and 9 ml of the sterilized water were inoculated and mixed in a container. After 5 minutes from the inoculation, 1 ml of each gauze for viable bacteria and coliform bacteria was dripped and wetted, and the surface of the stamp medium was contacted and cultured at 37 ° C. for 48 hours.

<Test results>
As shown in FIG. 4, in “4-1”, viable bacteria were confirmed to be settled in about 75% of the medium surface, whereas in “4-2”, 72 viable colonies were found, “4- In "3", 15 viable colonies were confirmed, and in "4-4", no viable colonies were observed. That is, it was confirmed that the sterilizing effect of the sterilizing water of the present invention is further increased by increasing the concentration from 100 times to 50 times, and further 33 times. It was also confirmed that the bactericidal effect was significantly increased by adding sodium hypochlorite.

[Example 4]
<Purpose of test>
A comparative test was conducted to confirm the presence or absence of the bactericidal effect in the high contamination area when chlorine was added to the sterilized water of the present invention.
<Adjustment of sterilizing water>
The mixing ratio of the sterilizing water used in this example is as shown in FIG. Moreover, as conventional sterilizing chlorine water for comparison, “5-1” is obtained by adding 0.1% by weight (120 ppm) of sodium hypochlorite to RO water, and sodium hypochlorite is added to RO water. Chlorine water added with 0.2 wt% (240 ppm) was prepared as “5-2”.
In addition, 100 ppm or more of sodium hypochlorite is a density | concentration area | region utilized at the time of the sterilization process in the highly contaminated area | regions, such as washing of the sheets and clothes in a hospital, a toilet, and a filth treatment.
<Adjustment of test bacterial solution>
As the test bacterial solution, the above-prepared “10-A” (10-fold diluted test bacterial solution) was used.
<Test operation>
1 ml of the test bacterial solution “10-A” and 9 ml of the sterilized water were inoculated and mixed in a container. After 5 minutes from the inoculation, 1 ml of each gauze for viable bacteria and coliform bacteria was dripped and wetted, and the surface of the stamp medium was contacted and cultured at 37 ° C. for 48 hours.

<Test results>
In “5-1”, viable bacteria were confirmed to be settled in about 95% of the medium surface, whereas in “5-4”, 42 viable colonies were confirmed, and “5-2”, “5” In “-3”, “5-5”, and “5-6”, no colonies were confirmed in both the living bacteria and the coliform group. That is, it was confirmed that the sterilizing effect of the sterilizing water of the present invention is further increased by increasing the concentration from 100 times to 50 times, and further 33 times. It was also confirmed that the bactericidal effect was significantly increased by adding sodium hypochlorite.

[Example 5]
<Purpose of test>
A comparative test was conducted to confirm the presence or absence of the bactericidal effect of the storage ice made by freezing the sterilized water of the present invention.
<Adjustment of sterilizing water>
The mixing ratio of the sterilizing water used in this example is as shown in FIG.
<Adjustment of test bacterial solution>
The prepared “30-B” (300-fold diluted test bacterial solution) was used as the test bacterial solution.
<Test operation>
The produced sterilized water was frozen in a freezer for 2 days, and 9 ml of sterilized water obtained by thawing the ice made and 1 ml of the test bacterial solution “30-B” were inoculated and mixed in a container. After 5 minutes from the inoculation, 1 ml of each gauze for viable bacteria and coliform bacteria was dripped and wetted, and the surface of the stamp medium was contacted and cultured at 37 ° C. for 48 hours.

<Test results>
As shown in FIG. 6, in any sterilized water, viable bacteria and coliforms were not detected, and the sterilizing effect could be confirmed. Therefore, it was proved that the ice obtained by freezing the sterilized water of the present invention can be used for the purpose of sterilizing the captured seafood, maintaining the freshness, or reducing the original odor of the seafood.

[Example 6]
<Purpose of test>
In the poultry industry, organic phosphorus, carbamate, and pyrethreite insecticides are used as a control. Resistance occurs in the spider mite and its effect is lost. Therefore, at present, there is no insecticide superior to the insecticide, and there is a concern that it is difficult to remove the spider mite. In view of such problems, a comparative test for confirming the presence or absence of an insecticidal effect in the sterilized water of the present invention was performed in this example.
<Adjustment of sterilizing water>
The mixing ratio of the sterilizing water used in this example is as shown in FIG.
<Test object>
Spider mite (length of about 1mm)
<Test operation>
Put one of the spider mites into a petri dish container, check when the spider mite has moved to the bottom of the petri dish, remove the lid, spray the sterilized water produced in the petri dish with an atomizer (atomizer), quickly cover and observe . The same test was conducted by dividing this into three times.

<Test results>
The spider mite sprayed with “7-1” and “7-2” to which the thickener is added starts to move on the water droplet surface of 0.2 mm or less attached to the entire surface of the petri dish, and as it moves about 30-50 mm, The lower half of the body and the forefoot part of the skin of the lower body part, and the water drops gradually attached to the back of the epidermis. Wakumodani repeated both left and right movements several times. When the oval water droplets adhering to the spider mite became longer than 1 mm in the middle, the oval water droplets were cut below that, and every time they moved forward, the oval water droplets increased again to 1 mm or more, and this state was repeated several times. In addition, after 3 minutes from the start of observation, the movement gradually became dull and the oval water droplets grew to 1 mm or more or were cut more slowly than before. From 5 minutes after the start of observation, the movement became minute and the elliptical water droplets grew to nearly 2 mm, and then the urticae stopped moving forward. At that time, the water droplets instantly wrapped the whole body from the head of the white mite to the front foot. After that, the stationary state continued, and after about 4 hours had passed until the water droplets wrapped around the mite were dried, the death of the mite was confirmed.

  As described above, by mixing a viscous agent with the sterilized water of the present invention, viscosity is imparted as a molecular group of water. For example, by spraying on mites, water droplets of water molecules, or a lump of water It is presumed that it is formed so as to surround the entire surface of the mite or to wrap the entire surface of the mite, and to cause damage (repellent effect, suffocation effect) to pests and mites. Therefore, the repellent and insecticidal effect with respect to pests, such as a spider mite, can be expected by applying as an insecticide for poultry farming.

[Example 7]
Using the sterilizing water of the present invention to which chlorine was added, a corrosion test for metals (irons) was performed.
<Adjustment of sterilizing water>
The mixing ratio of the sterilizing water used in this example is as shown in FIG. In addition, as conventional sterilizing chlorine water for comparison, sodium hypochlorite in RO water is 0.016 wt% (10 ppm), 0.050 wt% (30 ppm), 0.100 wt% (60 ppm). , 0.200 wt% (120 ppm), 0.400 wt% (240 ppm), and 1.000 wt% (600 ppm).
<Test operation>
An iron nail (with plating, 80 mm length) was used as an object to be corroded, and 20 cc of each sterilizing water and sterilizing chlorine water was put in a test tube, and the iron nail was immersed therein. After 60 days from the start of the test, the corrosion state was visually and visually evaluated. The evaluation criteria were evaluated as “corrosion” when the minute corrosion state was visually confirmed. Moreover, the thing which cannot confirm the minute corrosion state visually was evaluated as "no corrosion."

<Test results>
As shown in FIG. 8, sodium hypochlorite corroded the iron nail even at a low concentration of 10 ppm. Moreover, although corrosion occurred in “8-11” and “8-12”, corrosion could not be confirmed for the other sterilized water. Therefore, it was confirmed that the sterilizing water of the present invention containing chlorines is effective as a means for preventing or reducing the corrosiveness of metals.

[Example 8]
<Purpose of test>
Currently, it is a problem that food poisoning bacteria such as Campylobacter and Salmonella cause food poisoning to humans through livestock products, especially chicken eggs and chicken, but bacterial contamination of chicken such as broilers is not supported at the farm stage. There are reports that 70% of commercial chicken is contaminated, especially for Campylobacter. In addition, in the treatment process at the poultry treatment plant, contamination with sterilizing washing water is regarded as a problem as a cause of cross contamination of bacteria from contaminated chicken flocks.
In view of the above, a comparative experiment was performed to confirm the presence or absence of a sterilizing effect when the sterilizing water of the present invention was applied to the sterilizing cleaning water.
<Adjustment of sterilizing water>
The mixing ratio of the sterilizing water used in this example is as shown in FIG.
<Adjustment of test bacterial solution>
Salmonella Infantis was cultured in BHI medium, and 1.2 × 10 ⁹ CFU / ml of fungus was used.
<Test operation>
The sterilizing water was diluted with pure water so as to be 25 times and 27 times liquid. In addition, 10% organic substance (Fetal Bovine Serum: hereinafter referred to as “FBS”) was added to the 27-fold diluted solution for the test.
-Test for 25-fold diluted solution-
(1) First, 1 ml of S. Infantis (Salmonella) was added to a stomacher bag containing chicken cut into 10 g, and 6 pieces were brought into contact for 5 minutes.
(2) Add 40 ml of sterilized water adjusted to 25-fold dilution, an aqueous solution of sodium hypochlorite (effective chlorine concentration 200 ppm) as a comparative control, and pure water to another stomacher bag prepared in (1) above. Allowed to stand for 10 minutes.
(3) Take out contaminated chicken from three bags out of the bags prepared in (2) above (represented as post-disinfection solution a), rinse with 40 ml of pure water in a stomacher bag, and take out the contaminated chicken (each , Written after rinse a)
(4) In (3) above, 20 ml of phosphate buffered saline (PBS) was placed in a stomacher bag, and an emulsion was prepared with a back mixer (each indicated as Emulsion a).
(5) Contaminated chicken was removed from the remaining three stomacher bags treated up to (2) above, and 10 g of non-contaminated chicken was newly added and allowed to stand for 5 minutes.
(6) Uncontaminated chicken was taken out of the bag, 40 ml of pure water was placed in a stomacher bag and rinsed, and then the non-contaminated chicken was taken out (denoted as post-rinse solution b).
(7) In (6) above, 20 ml of PBS was placed in a stomacher bag, and an emulsion was prepared with a back mixer (each indicated as emulsion b).
(8) Each specimen was serially diluted 10-fold with PBS, inoculated into DHL agar plate medium, cultured at 37 ° C. for 24 hours, and the number of surviving Salmonella in the material was measured from the colonies that appeared.
-Test for 27-fold diluted solution-
(9) 1 ml of S. Infantis (Salmonella) was added to a stomacher bag containing chicken cut into 10 g, and contacted for 5 minutes to prepare 6 pieces.
(10) 27-fold diluted sterilized water, an aqueous solution of sodium hypochlorite (effective chlorine concentration 180 ppm) as a comparative control, and pure water are added to the stomacher bag prepared in (9) above for 10 minutes. Left to stand.
Hereinafter, tests similar to the above (3) to (8) were performed. Further, an experiment was conducted by adding 10% FBS as an organic substance to each test aqueous solution.

<Test results>
As shown in FIG. 9 (b), in the presence of organic matter (10% FBS), hypochlorous acid-based disinfectant was not able to suppress the spread of bacteria to the added meat pieces, but the suppression was confirmed with the sterilized water of the present invention. It was done. Moreover, when each test aqueous solution was put into contaminated chicken meat and meat pieces were put into each test aqueous solution after taking out the contaminated meat, bacteria did not adhere to the added meat (below the detection limit). Thereby, a cross-contamination prevention effect can be produced by using the sterilized water of the present invention as sterilizing washing water in a fresh food processing factory.

[Example 9]
<Purpose of test>
In order to prevent infectious diseases without using vaccines such as highly pathogenic avian influenza virus (HPAIV), foot-and-mouth disease, and parvovirus, it is important to enhance biosecurity. Therefore, in order to demonstrate the application as a new antiviral active agent, a comparative experiment was conducted to evaluate the antiviral activity of avian influenza virus (AIV) and goose parvovirus (GPV) using the sterilized water of the present invention.
<Adjustment of sterilizing water>
The mixing ratio of the sterilizing water used in this example is as shown in FIG. Moreover, the supernatant obtained by centrifuging with the aqueous solution which mixed 2.0% of the produced germicidal water, 2500 ppm of chlorine dioxide, and 0.5% of sodium carbonate each weight ratio was made into test aqueous solution.
<Test object>
As the goose parvovirus, GPV-IHC (DE15DEF25 2011/2/23) and avian influenza virus (AIV: H7N1 subtype) were used.
<Test operation>
450 μl of the test aqueous solution prepared in the sample tube and 100 μl of virus were added and reacted, and 450 μl of 1M Tris-HCl pH 7.2 was further added to adjust the reaction time. Next, the prepared 10 ⁻¹ reaction solution is diluted 10-fold serially (Virus: MM = 60 μl: 540 μl, MM indicates a maintenance medium), and cultivated Muscovy duck embryo fibroblast (MDEF) 96-well plates were inoculated. The cytopathic effect (CPE) was observed under a microscope until 7 days after the inoculation, and the 50% tissue culture infectious amount per ml (TCID ₅₀ / ml) was determined.

  In addition, since disinfectants generally lose their effects in the presence of organic matter, fetal bovine serum (FBS) 20% is used as the organic matter, and the effect of the supernatant test aqueous solution in the presence of FBS is also present. Examined. After adding 200 μl of FBS to 450 μl of the test aqueous solution prepared above, 100 μl of virus was inoculated, and 250 μl of 1M Tris-HCl pH 7.2 was added after a certain sensitization.

A test solution containing virus and MDEF were prepared in the following maintenance medium (MM) or growth medium (GM). As a medium for virus preparation or cell culture, 9.4 g of Eagle's minimum essential medium (manufactured by Nissui Pharmaceutical Co., Ltd.) and 3.0 g of Tryptose Phosphate Broth (TPB: Difco laboratories, Detroit, MI, USA) in 1000 ml of distilled water. After dissolution and autoclaving, 7% NaHCO ₃ , 200 mM L-glutamin, penicillin and streptomycin are added to final concentrations of 3%, 1%, 100 units / ml, and 100 μg / ml, respectively, and a maintenance medium (hereinafter referred to as MM). Then, 5% FBS was added to MM to obtain a growth medium (hereinafter referred to as GM). MDEF (1.0 × 10 6 cells / ml) was seeded at 15 ml in a 75 cm ² tissue culture flask or 200 μl / well in a 96-well tissue culture plate, and cultured at 37 ° C. in a carbon dioxide incubator. Remove GM from MDEF sheeted in 75 cm ² tissue culture flask, inoculate with virus, adsorb for 1 hour, add 15 ml of MM, and infectious culture solution 7 days after cytopathic effect (CPE) is marked Was recovered. The collected infection culture solution was centrifuged at 2500 rpm for 15 minutes at 4 ° C., and the resulting supernatant was used as a test virus.

The virus titer was measured by inoculating a 96-well tissue culture plate with 4 wells of each diluted dilution of the virus solution 10 times with MM. Before inoculation, GM was removed from MDEF, and 100 μl of diluted virus solution was inoculated therein. The CPE at 7 days after the inoculation was observed, and the virus titer (50% tissue culture infectious amount per ml: TCID ₅₀ / ml) was calculated from the number of positive holes at each dilution by the Behrens Kerber method. ₁₀ ).

<Test results>
Assuming that an index difference (inhibition index, ie, control virus titer index-treated virus titer index) of 2.8 or more is effective as compared with the untreated control, as shown in FIG. The anti-AIV activity was confirmed in the sterilized water of 5 minutes after sensitization for 5 seconds. Moreover, the effect was acquired with respect to GPV with high resistance by the sensitization for 30 seconds or more. In general, the antiseptic solution has its resistance suppressed in the presence of an organic substance, so the test was performed in the presence of 20% fetal bovine serum (FBS), but as shown in the figure, no decrease in the antiviral effect was observed. Therefore, it is shown that the sterilized water of the present invention can be used as a biosecurity strengthening material having excellent antiviral activity, and is presumed to be promising as a preventive measure against infectious diseases.

  The disinfectant of the present invention has an antibacterial, antifungal, antiviral and deodorizing action excellent in disinfecting and virus inactivation due to dissolution of biofilm, so that it can be used as a disinfectant in livestock breeding facilities. It can be used as sterilization washing water in a food processing factory. Further, the sterilized water can be made into ice and can be used as storage ice (including sherbet-like) for maintaining freshness. Furthermore, it can also be used as a hygiene control agent for improving and cultivating insects and preventing insects for the purpose of reducing pathogens and supplementing minerals during agriculture and plant cultivation. Moreover, it can be used as a household product as a laundry detergent, an antibacterial / deodorant such as a hand towel or a cloth, a cooking appliance or an antibacterial detergent around the kitchen. Moreover, by adjusting to an appropriate dilution concentration (pH 9.5 to 12.5), it can be used as a hairdressing / beauty treatment solution (makeup remover) or a bathing agent with less irritation without causing rough skin. Furthermore, since it is immediately effective against bacteria and viruses, it can be used as a hand-washing sterilizing aqueous solution without rough skin or odor.

Claims (6)

Sea shell calcined calcium having an average particle size of 25 nm to 10 μm in the range of 0.01 to 30% by weight, carbonates containing at least one of sodium carbonate or potassium carbonate in the range of 0.01 to 30% by weight, citric acid Natural porous organic acid salt containing at least one of sodium, potassium citrate, sodium malate, sodium ascorbate and sodium acetate in the range of 0.01 to 30 % by weight , mainly composed of montmorillonite and vermiculite Bactericidal water characterized by dissolving clay mineral in pure water in the range of 0.01 to 30% by weight. The sterilizing water according to claim 1 , further comprising sodium hypochlorite or chlorine dioxide in the range of 0.01 to 30% by weight as chlorines . Furthermore, at least one selected from the group consisting of metroise, casein, gelatin, starch, cellulose, dextrin and hyaluronic acid is contained as a thickener in the range of 0.01 to 30% by weight. The sterilizing water according to 1 or 2. An antiviral agent, which is the sterilized water according to claim 1 or 2, or a medicine containing the sterilized water . The sterilizing water according to claim 1 or 2, wherein the sterilizing water is an aqueous solution containing the sterilizing water. An insecticide for poultry farming, which is the sterilized water according to claim 3 or a medicine containing the sterilized water .

Images