WO2010059862A2 - Compositions et procédés de génération d’unités pilo-sébacées - Google Patents

Compositions et procédés de génération d’unités pilo-sébacées Download PDF

Info

Publication number
WO2010059862A2
WO2010059862A2 PCT/US2009/065202 US2009065202W WO2010059862A2 WO 2010059862 A2 WO2010059862 A2 WO 2010059862A2 US 2009065202 W US2009065202 W US 2009065202W WO 2010059862 A2 WO2010059862 A2 WO 2010059862A2
Authority
WO
WIPO (PCT)
Prior art keywords
cells
composition
hair
dermal
skin
Prior art date
Application number
PCT/US2009/065202
Other languages
English (en)
Other versions
WO2010059862A3 (fr
Inventor
Lily Lee
Cheng Ming Chuong
Warren Garner
Ting Xin Jiang
Original Assignee
University Of Southern California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Southern California filed Critical University Of Southern California
Priority to US13/130,296 priority Critical patent/US20110321180A1/en
Publication of WO2010059862A2 publication Critical patent/WO2010059862A2/fr
Publication of WO2010059862A3 publication Critical patent/WO2010059862A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • C12N5/0628—Hair stem cells; Hair progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/092—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells hair cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/04—Screening or testing on artificial tissues
    • C12N2503/06—Screening or testing on artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Abstract

L'invention porte sur des compositions et des procédés de génération d’unités pilo-sébacées. Sous un aspect, l'invention comporte un échafaudage biocompatible et une quantité efficace de cellules précurseurs dermiques et épidermiques.
PCT/US2009/065202 2008-11-20 2009-11-19 Compositions et procédés de génération d’unités pilo-sébacées WO2010059862A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/130,296 US20110321180A1 (en) 2008-11-20 2009-11-19 Compositions and methods to generate pilosebaceous units

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11662008P 2008-11-20 2008-11-20
US61/116,620 2008-11-20

Publications (2)

Publication Number Publication Date
WO2010059862A2 true WO2010059862A2 (fr) 2010-05-27
WO2010059862A3 WO2010059862A3 (fr) 2010-08-19

Family

ID=41503722

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/065202 WO2010059862A2 (fr) 2008-11-20 2009-11-19 Compositions et procédés de génération d’unités pilo-sébacées

Country Status (2)

Country Link
US (1) US20110321180A1 (fr)
WO (1) WO2010059862A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9402876B2 (en) 2013-11-27 2016-08-02 Industrial Technology Research Institute Method and pharmaceutical composition for hair growth
US9827181B2 (en) 2013-06-05 2017-11-28 Industrial Technology Research Institute Method and pharmaceutical composition for hair growth
KR101807727B1 (ko) 2017-10-23 2018-01-18 차의과학대학교 산학협력단 인간-유래의 체세포로부터 역분화 만능 줄기세포의 제조방법

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9655930B2 (en) 2012-10-01 2017-05-23 Aderans Research Institute, Inc. Compositions and methods for producing reconstituted skin
CN103785064B (zh) * 2013-08-26 2015-02-25 济南磐升生物技术有限公司 一种皮肤模型及应用
US9592257B2 (en) * 2013-10-11 2017-03-14 MWV Cell, LLC Complete human skin organ generated from culture-expanded cells
US11021688B2 (en) 2015-10-21 2021-06-01 Indiana University Research Annd Technology Corporation Derivation of human skin organoids from pluripotent stem cells
US20200121760A1 (en) * 2017-01-04 2020-04-23 The Trustees Of The University Ofpennsylvania Methods for scar reduction by converting scar fibroblasts into adipocytes with hair follicle-derived signals
CN112294849B (zh) * 2020-11-04 2024-05-03 陕西中鸿科瑞再生医学研究院有限公司 用于生发的脱细胞基质-细胞复合颗粒及制备方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008288260B2 (en) * 2007-08-14 2014-10-02 Smith & Nephew Plc Scaffolds

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BOEHNKE ET AL: "Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents" EUROPEAN JOURNAL OF CELL BIOLOGY, WISSENSCHAFLICHE VERLAGSGESELLSCHAFT, STUTTGART, DE, vol. 86, no. 11-12, 13 November 2007 (2007-11-13), pages 731-746, XP022342142 ISSN: 0171-9335 *
HE LIJUAN ET AL: "Full-thickness tissue engineered skin constructed with autogenic bone marrow mesenchymal stem cells" SCIENCE IN CHINA SERIES C LIFE SCIENCES, vol. 50, no. 4, August 2007 (2007-08), pages 429-437, XP002563452 ISSN: 1006-9305 *
POWELL ET AL: "Influence of electrospun collagen on wound contraction of engineered skin substitutes" BIOMATERIALS, ELSEVIER SCIENCE PUBLISHERS BV., BARKING, GB, vol. 29, no. 7, 3 December 2007 (2007-12-03), pages 834-843, XP022394092 ISSN: 0142-9612 *
WILKINS LEON M ET AL: "Development of a bilayered living skin construct for clinical applications" BIOTECHNOLOGY AND BIOENGINEERING, vol. 43, no. 8, 1994, pages 747-756, XP002563453 ISSN: 0006-3592 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9827181B2 (en) 2013-06-05 2017-11-28 Industrial Technology Research Institute Method and pharmaceutical composition for hair growth
US9402876B2 (en) 2013-11-27 2016-08-02 Industrial Technology Research Institute Method and pharmaceutical composition for hair growth
KR101807727B1 (ko) 2017-10-23 2018-01-18 차의과학대학교 산학협력단 인간-유래의 체세포로부터 역분화 만능 줄기세포의 제조방법

Also Published As

Publication number Publication date
WO2010059862A3 (fr) 2010-08-19
US20110321180A1 (en) 2011-12-29

Similar Documents

Publication Publication Date Title
US20110321180A1 (en) Compositions and methods to generate pilosebaceous units
JP5600671B2 (ja) 毛小嚢及びデノボ乳頭の作製方法並びにインビトロ試験及びインビボ移植のためのそれらの使用
Mistriotis et al. Hair follicle: a novel source of multipotent stem cells for tissue engineering and regenerative medicine
KR20110022713A (ko) 인간 만능줄기세포로부터 인간 피부 대체물을 제조하는 방법
KR102251129B1 (ko) 모발 성장을 조정하기 위한 방법 및 조성물
US9592257B2 (en) Complete human skin organ generated from culture-expanded cells
US20120148541A1 (en) Compositions and methods to generate pilosebaceous units
US10398735B2 (en) Compositions and methods for producing reconstituted skin
US20220002678A1 (en) Composition for reconstituting human skin tissue having hair follicles, human skin tissue model animal, and production method thereof
EP1337624B1 (fr) Constructions cellulaires obtenues a partir de cellules souches mesenchymateuses, cellules pouvant deriver de celles-ci et leur utilisation
JP5301146B2 (ja) 特定の方法で培養されたケラチノサイト前駆細胞及びその製造方法
Malise Induction of hair follicles using neonatal mouse dermis and human keratinocytes: relevance for improved burn wound treatments
Chen K15 & Id3 expression in intact and regenerating adult vibrissae rodent hair follicles

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09764131

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 13130296

Country of ref document: US

122 Ep: pct application non-entry in european phase

Ref document number: 09764131

Country of ref document: EP

Kind code of ref document: A2