WO2010058294A1 - Souche hypocholestérolémiante de lactobacillus delbrueckii - Google Patents
Souche hypocholestérolémiante de lactobacillus delbrueckii Download PDFInfo
- Publication number
- WO2010058294A1 WO2010058294A1 PCT/IB2009/007681 IB2009007681W WO2010058294A1 WO 2010058294 A1 WO2010058294 A1 WO 2010058294A1 IB 2009007681 W IB2009007681 W IB 2009007681W WO 2010058294 A1 WO2010058294 A1 WO 2010058294A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- strain
- lactobacillus
- cholesterol
- lactobacillus delbrueckii
- cncm
- Prior art date
Links
- 241000186673 Lactobacillus delbrueckii Species 0.000 title claims abstract description 16
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 10
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 6
- 241000894007 species Species 0.000 claims description 6
- 235000014655 lactic acid Nutrition 0.000 claims description 5
- 239000004310 lactic acid Substances 0.000 claims description 5
- 238000008214 LDL Cholesterol Methods 0.000 claims description 4
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 4
- 239000008280 blood Substances 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 4
- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 claims 1
- 235000013336 milk Nutrition 0.000 abstract description 12
- 239000008267 milk Substances 0.000 abstract description 12
- 210000004080 milk Anatomy 0.000 abstract description 12
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 description 9
- 241000699670 Mus sp. Species 0.000 description 8
- 235000013365 dairy product Nutrition 0.000 description 8
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- 235000014048 cultured milk product Nutrition 0.000 description 6
- 241000186660 Lactobacillus Species 0.000 description 4
- 240000001046 Lactobacillus acidophilus Species 0.000 description 4
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 4
- 235000012000 cholesterol Nutrition 0.000 description 4
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000006872 mrs medium Substances 0.000 description 4
- 235000013618 yogurt Nutrition 0.000 description 4
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 3
- 244000199866 Lactobacillus casei Species 0.000 description 3
- 235000013958 Lactobacillus casei Nutrition 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000020183 skimmed milk Nutrition 0.000 description 3
- 101150037123 APOE gene Proteins 0.000 description 2
- 102100029470 Apolipoprotein E Human genes 0.000 description 2
- 101710095339 Apolipoprotein E Proteins 0.000 description 2
- 241001134770 Bifidobacterium animalis Species 0.000 description 2
- 241001608472 Bifidobacterium longum Species 0.000 description 2
- 241000186015 Bifidobacterium longum subsp. infantis Species 0.000 description 2
- 101100216294 Danio rerio apoeb gene Proteins 0.000 description 2
- 241000194031 Enterococcus faecium Species 0.000 description 2
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 2
- 241000186840 Lactobacillus fermentum Species 0.000 description 2
- 241000218588 Lactobacillus rhamnosus Species 0.000 description 2
- 241000194034 Lactococcus lactis subsp. cremoris Species 0.000 description 2
- 235000014962 Streptococcus cremoris Nutrition 0.000 description 2
- 239000008186 active pharmaceutical agent Substances 0.000 description 2
- 229940118852 bifidobacterium animalis Drugs 0.000 description 2
- 229940004120 bifidobacterium infantis Drugs 0.000 description 2
- 229940009291 bifidobacterium longum Drugs 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 230000002550 fecal effect Effects 0.000 description 2
- 230000002440 hepatic effect Effects 0.000 description 2
- 229940017800 lactobacillus casei Drugs 0.000 description 2
- 229940012969 lactobacillus fermentum Drugs 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 102000013918 Apolipoproteins E Human genes 0.000 description 1
- 108010025628 Apolipoproteins E Proteins 0.000 description 1
- 241000304886 Bacilli Species 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 241000186018 Bifidobacterium adolescentis Species 0.000 description 1
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 description 1
- 241000186016 Bifidobacterium bifidum Species 0.000 description 1
- 241000186012 Bifidobacterium breve Species 0.000 description 1
- RFSUNEUAIZKAJO-VRPWFDPXSA-N D-Fructose Natural products OC[C@H]1OC(O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-VRPWFDPXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 241000520134 Enterococcus mundtii Species 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000186713 Lactobacillus amylovorus Species 0.000 description 1
- 240000001929 Lactobacillus brevis Species 0.000 description 1
- 235000013957 Lactobacillus brevis Nutrition 0.000 description 1
- 241000218492 Lactobacillus crispatus Species 0.000 description 1
- 241001134659 Lactobacillus curvatus Species 0.000 description 1
- 241001647786 Lactobacillus delbrueckii subsp. delbrueckii Species 0.000 description 1
- 241000186606 Lactobacillus gasseri Species 0.000 description 1
- 240000002605 Lactobacillus helveticus Species 0.000 description 1
- 235000013967 Lactobacillus helveticus Nutrition 0.000 description 1
- 241001468157 Lactobacillus johnsonii Species 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 241000186604 Lactobacillus reuteri Species 0.000 description 1
- 241000186869 Lactobacillus salivarius Species 0.000 description 1
- 241000577554 Lactobacillus zeae Species 0.000 description 1
- 241000194041 Lactococcus lactis subsp. lactis Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- 241000191998 Pediococcus acidilactici Species 0.000 description 1
- 241000191996 Pediococcus pentosaceus Species 0.000 description 1
- 235000014969 Streptococcus diacetilactis Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000003816 axenic effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 229940002008 bifidobacterium bifidum Drugs 0.000 description 1
- 229940009289 bifidobacterium lactis Drugs 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229940054346 lactobacillus helveticus Drugs 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 229940001882 lactobacillus reuteri Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 235000015192 vegetable juice Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
Definitions
- the invention relates to a novel strain of Lactobacillus delbrueckii having blood-cholesterol-lowering properties .
- Lactobacillus casei (Brashears et al . , J Dairy Sci, 81, 2103-10, 1998; Kapila & Sinha, Indian J Med Sci, 60, 361-70, 2006; Kawase et al., J Dairy Sci, 83, 255-63, 2000), Lactobacillus fermentum (Gilliland & Walker, J Dairy Sci, 73, 905-11, 1990; Pereira et al., Appl Environ Microbiol, 69, 4743-52, 2003) , and especially Lactobacillus acidophilus (Gilliland et al .
- Lactobacillus delbrueckii which is one of the species most widely used in the manufacture of fermented milk products since it constitutes, in combination with Streptococcus thermophilus, the conventional "yoghurt symbiosis", was until now considered to be devoid of blood- cholesterol-lowering effects.
- Lactobacillus delbrueckii was administered to mice deficient in apolipoprotein E (ApoE KO mice) , no effect was observed, whether for fecal, hepatic or blood cholesterol (Portugal et al., Braz J Med Biol Res, 39, 629-35, 2006) .
- the inventors have now isolated a strain of Lactobacillus delbrueckii which, surprisingly, has blood- cholesterol-lowering properties.
- This strain also has the capacity for growth on milk and the propagation capacity of the Lactobacillus delbrueckii strains conventionally used in the manufacture of yoghurts, and can therefore be conveniently used in the manufacture of fermented milk products, and in particular of yoghurts.
- the present invention provides the Lactobacillus delbrueckii subspecies lactis strain deposited on 6 April 2007 under number 1-3741 with the CNCM (Collection Nationale de Cultures de Microorganismes) [French National Microorganism Culture Collection] , 25 rue du Do Budapest Roux, in Paris .
- CNCM Collection Nationale de Cultures de Microorganismes
- Morphology Gram-positive microorganism, more or less long, granular bacilli which are quite small.
- the present invention also provides a lactic ferment comprising the Lactobacillus delbrueckii subspecies lactis strain CNCM 1-3741 combined with one or more other strain (s) of lactic acid bacteria.
- Said other strain (s) may in particular belong to the following species: Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus casei subsp. casei, Lactobacillus casei subsp.
- Lactobacillus zeae Lactobacillus salivarius
- Lactobacillus lactis Lactobacillus helveticus
- Lactobacillus casei Lactobacillus paracasein Lactobacillus cremoris
- Lactobacillus rhamnosus Lactobacillus gasseri , Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus delbrueckii subsp. lactis, Lactobacillus delbrueckii subsp. delbrueckii, Lactobacillus delbrueckii subsp.
- Lactobacillus reuteri Lactobacillus amylovorus
- Lactobacillus crispatus Lactobacillus curvatus
- Lactobacillus johnsonii Lactobacillus fermentum
- Lactobacillus brevis Streptococcus thermophilus
- cremoris Enterococcus faecium, Pediococcus pentosaceus, Pediococcus acidilactici, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium lactis, Bifidobacterium animalis, Bifidobacterium animalis sp. lactis, Bifidobacterium infantis, Bifidobacterium adolescentis .
- the Lactobacillus delbrueckii subspecies lactis strain CNCM 1-3741 is combined with at least one strain of Lactobacillus delbrueckii bulgaricus and at least one strain of Streptococcus thermophilus .
- the present invention also provides a method for preparing a fermented product, which method is characterized in that it comprises the fermentation of a suitable medium with the CNCM 1-3741 strain, or with a lactic ferment in accordance with the invention, containing said strain.
- the medium used for the fermentation is milk or a milk-based medium, or a plant juice (fruit and/or vegetable juice) or a mixture of these media.
- the present invention also provides fermented products, in particular fermented milk products, containing bacteria of the CNCM 1-3741 strain, and optionally bacteria of one or more other strains of lactic acid bacteria.
- a fermented product in accordance with the invention contains, after fermentation, at least IxIO 6 CFU (colony- forming units) , preferably at least IxIO 7 CFU, and more preferably between IxIO 7 and IxIO 9 CFU, per ml of bacteria of the CNCM 1-3741 strain.
- IxIO 6 CFU colony- forming units
- the present invention also relates to the use of the CNCM 1-3741 strain, or of lactic ferments or of fermented products containing said strain, for obtaining products which induce a decrease in the blood cholesterol level, in particular the LDL cholesterol level.
- the 1-2273 strain is a strain which has a blood-cholesterol-lowering activity in vitro, but which cannot be readily used for obtaining fermented milk products since it grows very poorly on milk.
- the experimental protocol is the following:
- mice Forty apoE KO mice, 9 to 10 weeks old, were divided up into 4 groups of 10 mice. These mice were provided by Charles River Laboratories, L'Arbresle (France) .
- mice received, for 28 days, a lipid- enriched diet supplemented, by gavage, with: -300 ⁇ l/day of a culture containing 6 x 10 8 CFU of the DN 111 244 strain, obtained under the culture conditions on milk-MRS medium (group 1) ;
- control milk the composition of which is the following: skimmed milk (control) .
- the DN 112 001 and DN 111 244 (CNCM 1-3741) strains were cultured on MRS medium. They were subsequently resuspended in milk so as to obtain a milk containing 6 x 10 8 CFU of bacteria.
- the daily intake of the animals in the three groups was identical.
- the animals were weighed at the beginning of the experiment, and then at 5-day intervals over the course of the treatment. No significant difference between the various groups was observed.
- a medium made up of reconstituted skimmed milk 120 g of skimmed milk powder in one litre of distilled water, supplemented with 2 g/1 of yeast extract
- the fermentative activity of the strains, linked to their growth, is measured by continuously managing the pH of the growth medium.
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Epidemiology (AREA)
- Obesity (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dairy Products (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
L'invention porte sur une nouvelle souche de Lactobacillus delbrueckii présentant des propriétés hypocholestérolémiante et sur l'utilisation de ladite souche pour la fabrication de produits fermentés hypocholestérolémiants, en particulier de produits laitiers.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009801461515A CN102216444B (zh) | 2008-11-19 | 2009-11-19 | 德氏乳杆菌的降低血胆固醇菌株 |
| US13/129,255 US8652461B2 (en) | 2008-11-19 | 2009-11-19 | Blood-cholesterol-lowering strain of Lactobacillus delbrueckii |
| RU2011124924/10A RU2539785C2 (ru) | 2008-11-19 | 2009-11-19 | ШТАММ Lactobacillus delbrueckii, СНИЖАЮЩИЙ СОДЕРЖАНИЕ ХОЛЕСТЕРИНА В КРОВИ |
| ES09796798T ES2396501T3 (es) | 2008-11-19 | 2009-11-19 | Cepa de Lactobacillus delbrueckii que disminuye el colesterol en sangre |
| EP09796798A EP2366017B1 (fr) | 2008-11-19 | 2009-11-19 | Souche hypocholestérolémiante de lactobacillus delbrueckii |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0806467A FR2938552B1 (fr) | 2008-11-19 | 2008-11-19 | Souche hypocholesterolemiante de lactobacillus delbrueckii |
| FR0806467 | 2008-11-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2010058294A1 true WO2010058294A1 (fr) | 2010-05-27 |
Family
ID=40457356
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/IB2009/007681 WO2010058294A1 (fr) | 2008-11-19 | 2009-11-19 | Souche hypocholestérolémiante de lactobacillus delbrueckii |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8652461B2 (fr) |
| EP (1) | EP2366017B1 (fr) |
| CN (1) | CN102216444B (fr) |
| ES (1) | ES2396501T3 (fr) |
| FR (1) | FR2938552B1 (fr) |
| RU (1) | RU2539785C2 (fr) |
| WO (1) | WO2010058294A1 (fr) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286407A (zh) * | 2011-08-03 | 2011-12-21 | 甘肃农业大学 | 低温发酵的德氏保加利亚乳杆菌及用其制备酸奶的方法 |
| WO2015093997A1 (fr) * | 2013-12-20 | 2015-06-25 | Instytut Biotechnologii Przemysłu Rolno- Spożywczego Im. Prof.Wacława Dąbrowskiego | Nouvelle souche de bactérie lactobacillus delbrueckii et son utilisation pour la production de pollen d'abeille |
| WO2016029210A1 (fr) * | 2014-08-22 | 2016-02-25 | Grech Co. Llc | Produit nutraceutique diminuant le taux de cholestérol comprenant du blé noir brut et boisson lactée de culture |
| US9312863B2 (en) | 2011-08-17 | 2016-04-12 | Broadcom Corporation | Wave clocking |
| WO2019121698A1 (fr) | 2017-12-19 | 2019-06-27 | Compagnie Gervais Danone | Compositions laitières fermentées et procédés de préparation de celles-ci |
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| EP3006555A4 (fr) * | 2013-05-31 | 2017-01-11 | Meiji Co., Ltd. | Lait fermenté qui ne subit pas d'augmentation du taux d'acide, et son procédé de production |
| CN109666600B (zh) * | 2018-12-03 | 2021-01-26 | 杭州娃哈哈科技有限公司 | 一株产功能氨基酸的德氏乳杆菌乳亚种及其用途 |
| CN110331111A (zh) * | 2019-07-26 | 2019-10-15 | 浙江一鸣食品股份有限公司 | 一种辅助降血脂功能的乳酸菌组合物及其应用 |
| KR102128098B1 (ko) * | 2020-03-23 | 2020-06-30 | 주식회사 종근당바이오 | 락토바실러스 델브루키 subsp. 락티스 CKDB001 균주, 및 이를 포함하는 비알코올성 지방간의 예방, 개선, 또는 치료용 조성물 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0101209A2 (fr) | 1982-08-06 | 1984-02-22 | Kabushiki Kaisya Advance | Produits ayant une activité du type hypocholestérol et/ou hypotriglycéride |
| EP0858501A1 (fr) | 1995-10-31 | 1998-08-19 | Compagnie Gervais-Danone | Ferments lactiques, et leur utilisation pour l'obtention de produits hypocholesterolemiants |
| EP1251860A1 (fr) | 1999-12-20 | 2002-10-30 | Compagnie Gervais Danone | Activite somnogenique de bacteries lactiques non pathogenes |
| WO2007029773A1 (fr) | 2005-09-08 | 2007-03-15 | Kabushiki Kaisha Yakult Honsha | Inhibiteur d’absorption du cholestérol |
| WO2007108583A1 (fr) | 2006-03-22 | 2007-09-27 | Heuyn Kil Shin | Bactérie lactique permettant la réduction de cholestérol dans le sang et procédé de production d'oeufs à cholestérol réduit utilisant la bactérie |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2072692C1 (ru) * | 1994-04-13 | 1997-01-27 | Московский научно-исследовательский институт эпидемиологии и микробиологии им.Г.Н.Габричевского | Штамм бактерий lactobacillus helveticus, обладающий способностью снижать уровень холестерина в крови и используемый для получения препаратов и продуктов питания для профилактики и лечения гиперхолестеринемий |
| JP2001097870A (ja) * | 1999-09-30 | 2001-04-10 | Snow Brand Milk Prod Co Ltd | 乳酸菌を利用した胆汁酸の吸着方法 |
| CN1317385C (zh) * | 2003-05-30 | 2007-05-23 | 上海光明乳业股份有限公司 | 干酪乳杆菌Bd－II菌株及其在降低血脂方面的应用 |
-
2008
- 2008-11-19 FR FR0806467A patent/FR2938552B1/fr not_active Expired - Fee Related
-
2009
- 2009-11-19 CN CN2009801461515A patent/CN102216444B/zh not_active Expired - Fee Related
- 2009-11-19 EP EP09796798A patent/EP2366017B1/fr not_active Not-in-force
- 2009-11-19 WO PCT/IB2009/007681 patent/WO2010058294A1/fr active Application Filing
- 2009-11-19 RU RU2011124924/10A patent/RU2539785C2/ru not_active IP Right Cessation
- 2009-11-19 ES ES09796798T patent/ES2396501T3/es active Active
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0101209A2 (fr) | 1982-08-06 | 1984-02-22 | Kabushiki Kaisya Advance | Produits ayant une activité du type hypocholestérol et/ou hypotriglycéride |
| EP0858501A1 (fr) | 1995-10-31 | 1998-08-19 | Compagnie Gervais-Danone | Ferments lactiques, et leur utilisation pour l'obtention de produits hypocholesterolemiants |
| EP1251860A1 (fr) | 1999-12-20 | 2002-10-30 | Compagnie Gervais Danone | Activite somnogenique de bacteries lactiques non pathogenes |
| WO2007029773A1 (fr) | 2005-09-08 | 2007-03-15 | Kabushiki Kaisha Yakult Honsha | Inhibiteur d’absorption du cholestérol |
| WO2007108583A1 (fr) | 2006-03-22 | 2007-09-27 | Heuyn Kil Shin | Bactérie lactique permettant la réduction de cholestérol dans le sang et procédé de production d'oeufs à cholestérol réduit utilisant la bactérie |
Non-Patent Citations (16)
| Title |
|---|
| A. SIBEL ALKALIN ET AL: "Influence of yogurt and acidophilus yogurt on", J. DAIRY SCI., vol. 80, 1997, pages 2721 - 2725, XP002521452 * |
| AKALIN ET AL., J DAIRY SCI, vol. 80, 1997, pages 2721 - 5 |
| ANDERSON; GILLILAND, J AM COLL NUTR, vol. 18, 1999, pages 43 - 50 |
| BRASHEARS ET AL., J DAIRY SCI, vol. 81, 1998, pages 2103 - 10 |
| GILLILAND ET AL., APPL ENVIRON MICROBIOL, vol. 49, 1985, pages 377 - 81 |
| GILLILAND; WALKER, J DAIRY SCI, vol. 73, 1990, pages 905 - 11 |
| GUPTA ET AL., INT J FOOD MICROBIOL, vol. 29, 1996, pages 105 - 9 |
| KAPILA; SINHA, INDIAN J MED SCI, vol. 60, 2006, pages 361 - 70 |
| KAWASE ET AL., J DAIRY SCI, vol. 83, 2000, pages 255 - 63 |
| L.R. PORTUGAL ET AL,: "Effect of Lactobacillus delbrueckii on cholesterol metabolism in germ-free mice and on atherogenesis in apolipoprotein E knock-out mice", BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, vol. 39, 2006, pages 629 - 635, XP002521455 * |
| LIN ET AL., J DAIRY SCI, vol. 72, 1989, pages 2885 - 99 |
| MASKI TERAHARA ET AL: "Preventive effect of Lactobacillus delbrueckii subsp. bulgaricus on teh oxidation of LDL.", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 64, no. 9, 2000, pages 1868 - 1873, XP002521454 * |
| PEREIRA ET AL., APPL ENVIRON MICROBIOL, vol. 69, 2003, pages 4743 - 52 |
| PORTUGAL ET AL., BRAZ J MED BIOL RES, vol. 39, 2006, pages 629 - 35 |
| XIAO ET AL., J DAIRY SCI, vol. 86, 2003, pages 2452 - 61 |
| YOUITCHI TAMAI ET AL.: "effects of milk fermented by culturing with various lactic acid bacteria and a yeast on serum cholesterol level in rats", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 81, no. 2, 1996, pages 181 - 182, XP002521453 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286407A (zh) * | 2011-08-03 | 2011-12-21 | 甘肃农业大学 | 低温发酵的德氏保加利亚乳杆菌及用其制备酸奶的方法 |
| US9312863B2 (en) | 2011-08-17 | 2016-04-12 | Broadcom Corporation | Wave clocking |
| WO2015093997A1 (fr) * | 2013-12-20 | 2015-06-25 | Instytut Biotechnologii Przemysłu Rolno- Spożywczego Im. Prof.Wacława Dąbrowskiego | Nouvelle souche de bactérie lactobacillus delbrueckii et son utilisation pour la production de pollen d'abeille |
| JP2016508037A (ja) * | 2013-12-20 | 2016-03-17 | インスティテュト、バイオテクノロジー、プシェミスウ、ロルノ−スポジチェゴ、イム．プロフ．バツワバ、ドンブロフスキエゴＩｎｓｔｙｔｕｔ Ｂｉｏｔｅｃｈｎｏｌｏｇｉｉ Ｐｒｚｅｍｙｓｌｕ Ｒｏｌｎｏ−Ｓｐｏｚｙｗｃｚｅｇｏ Ｉｍ． Ｐｒｏｆ． Ｗａｃｌａｗａ Ｄａｂｒｏｗｓｋｉｅｇｏ | ラクトバチルス・デルブリッキー菌の新規株、およびビーポーレンの生産のためのその使用 |
| WO2016029210A1 (fr) * | 2014-08-22 | 2016-02-25 | Grech Co. Llc | Produit nutraceutique diminuant le taux de cholestérol comprenant du blé noir brut et boisson lactée de culture |
| WO2019121698A1 (fr) | 2017-12-19 | 2019-06-27 | Compagnie Gervais Danone | Compositions laitières fermentées et procédés de préparation de celles-ci |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2539785C2 (ru) | 2015-01-27 |
| ES2396501T3 (es) | 2013-02-22 |
| FR2938552B1 (fr) | 2010-12-03 |
| CN102216444B (zh) | 2013-06-19 |
| RU2011124924A (ru) | 2012-12-27 |
| EP2366017A1 (fr) | 2011-09-21 |
| EP2366017B1 (fr) | 2012-09-26 |
| CN102216444A (zh) | 2011-10-12 |
| FR2938552A1 (fr) | 2010-05-21 |
| US8652461B2 (en) | 2014-02-18 |
| US20110305679A1 (en) | 2011-12-15 |
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