WO2010055939A1 - Plaque de puits munie de bouchons - Google Patents

Plaque de puits munie de bouchons Download PDF

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Publication number
WO2010055939A1
WO2010055939A1 PCT/JP2009/069438 JP2009069438W WO2010055939A1 WO 2010055939 A1 WO2010055939 A1 WO 2010055939A1 JP 2009069438 W JP2009069438 W JP 2009069438W WO 2010055939 A1 WO2010055939 A1 WO 2010055939A1
Authority
WO
WIPO (PCT)
Prior art keywords
cap
well
plate
well plate
caps
Prior art date
Application number
PCT/JP2009/069438
Other languages
English (en)
Japanese (ja)
Inventor
精久 彌田
啓男 和田
Original Assignee
信和化工株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 信和化工株式会社 filed Critical 信和化工株式会社
Publication of WO2010055939A1 publication Critical patent/WO2010055939A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation

Definitions

  • the present invention relates to a well plate provided with a plurality of wells, and more specifically, a cap in which a well member provided with a plurality of wells and a plurality of caps individually closing each well are arranged corresponding to the arrangement of the wells.
  • the present invention relates to a well plate with a cap combined with a plate member.
  • a well plate in which a plurality of wells are regularly arranged has been used in order to store a large number of samples used in instruments such as various chromatographic autosamplers and microplate readers.
  • the well plate is a cap plate in which a plurality of caps for individually sealing each well are arranged corresponding to the arrangement of the wells in order to prevent evaporation of the solvent and mixing of foreign substances when storing the contained sample.
  • the well plate in order to efficiently sample with the sampling needle, the well plate preferably sucks the sample by passing the sampling needle through the cap well, and is made of silicon rubber or the like.
  • a sample is accommodated in each well, and a cap plate is arranged at a predetermined position on the well, and the well is closed by the cap by pushing from above.
  • This occlusion increases the internal pressure of the well by pushing the cap or the evaporation of the sample, thereby gradually increasing the internal pressure and pushing up the cap.
  • the pushing up and opening of the cap causes problems such as hindering the operation of the autosampler due to the displacement of the cap position and changes in the sample concentration due to evaporation of the sample solvent.
  • a multi-well plate for storing samples which is a multi-well plate for freezing and storing samples, and the lid used has an internal lid made of an elastomer sheet (
  • the multiwell plate cover and assembly also includes a lid and a gasket, the lid has spring means essential for the force applied uniformly to the face of the gasket to seal the multiwell plate, and the lid is mechanical
  • a configuration having components for connection with a manual operation and a multiwell plate see, for example, Patent Document 2.
  • a lid for the multi-well plate body having at least one channel formed therethrough, the lid positioned adjacent to the gasket member, and the channel positioned to draw the gasket toward the lid
  • a configuration has been proposed that includes applying sufficient negative pressure, placing a lid on the multi-well plate body and releasing the negative pressure from the channel while the negative pressure continues to be applied to the channel (e.g., (See Patent Document 3).
  • Patent Document 4 can suppress an increase in internal pressure caused by pushing an independent cap into each well from above, but the work of fitting caps one by one to each well is extremely complicated. Is not efficient.
  • the problem of cap lifting and removal can be reduced to some extent by increasing the length of the cap.
  • the internal volume of the well is reduced, which is not preferable.
  • the present invention has been made in view of the above-described problems of conventional well plates with caps, and can be closed with a cap with a simple configuration. After the well plate is closed with a cap plate member, the cap plate member is a well plate.
  • An object of the present invention is to provide a well plate with a cap which is less likely to float from the plate.
  • Another object of the present invention is to provide a well plate with a cap that can effectively utilize the internal volume of the well.
  • the present invention further provides a well plate with a cap capable of reliably closing the well with the cap by a simple operation of disposing the cap plate member on the well member and pressing the cap plate with a roller. For the purpose.
  • the first invention is in a well plate with a cap formed by combining a well member provided with a plurality of wells and a flexible cap plate member in which a plurality of caps individually closing each well are arranged corresponding to the arrangement of the wells,
  • the inner diameter of the well and the outer diameter of the cap are formed so that the inner diameter of the well is smaller than the outer diameter of the cap so that the cap can be pushed into the well.
  • the cap member is integrally formed by connecting a plurality of caps by a plate portion,
  • the cap is a well plate with a cap characterized in that the upper end portion has an opening, the lower bottom portion is formed so that a needle can be inserted, and the outer surface has a longitudinal groove extending from the lower end to an intermediate height.
  • Embodiments of the first invention are as follows.
  • the material of the cap member is natural rubber, synthetic rubber, silicon rubber, or thermoplastic plastic.
  • the material of the cap member is a thermoplastic elastomer.
  • the length of the longitudinal groove is not less than 1/2 of the height of the cap and not more than 9/10.
  • the longitudinal groove has a semicircular cross section.
  • the longitudinal groove has a triangular cross section.
  • the length of the longitudinal groove is not less than 1/2 of the height of the cap and not more than 9/10.
  • the surface of the cap is coated with ethylene trifluoride or tetrafluoroethylene.
  • the second invention is A well plate with a cap formed by combining a well member provided with a plurality of wells and a flexible cap plate member in which a plurality of caps individually closing each well are arranged corresponding to the arrangement of the wells.
  • the inner diameter and the outer diameter of the cap are formed so that the inner diameter of the well is smaller than the outer diameter of the cap so that the cap can be pushed into the well, and the cap member is integrally formed by connecting a plurality of caps by the plate portion,
  • the cap has an opening at the upper end, a lower bottom formed so that a needle can be inserted, and a longitudinal groove extending from the lower end to the intermediate height on the outer surface,
  • a method of closing a well plate with a cap wherein the cap plate member is disposed on the well member, and the well is closed with a cap by pressing the cap plate with a roller.
  • the well plate with a cap of the present invention it is possible to close the cap with a simple structure, and to obtain an effect that the cap plate member is not lifted from the well plate after the well plate is closed by the cap plate member. it can. According to the present invention, it is also possible to obtain an effect of configuring a well plate with a cap that can effectively use the internal volume of the well.
  • FIG. 1 It is a perspective view of the well member of the well plate with a cap of one form of the present invention. It is the perspective view seen from the front side of the cap plate member of the well plate with a cap of one form of the present invention. It is the perspective view seen from the back side of the cap plate member of the well plate with a cap of one form of the present invention. It is the partially cutaway enlarged view of the cap plate member of the well plate with a cap of one form of the present invention.
  • the well plate with a cap includes a well member 10 and a cap plate member 30.
  • the well member 10 is made non-flexible by molding a material such as polystyrene, polypropylene, acrylonitrile, butanediene, styrene copolymer (ABS) or the like.
  • the well member 10 has, for example, 96 wells 12 in 8 rows and 12 columns, and the wells 12 are arranged in the well plate portion 14.
  • the well plate part 14 has a side part 16 around the well plate part 14 in order to keep the well plate part 14 at a constant height.
  • the well 12 has an open top cylindrical shape and has a well bottom 18.
  • the inner diameter of the well 12 is, for example, 8 mm, and the depth of the well 12 varies depending on a desired accommodation amount, for example, 11 mm.
  • the cap plate member 30 includes a cap 32 in a cap plate portion 31 and a position corresponding to the position of the well 12.
  • the cap 32 is formed from a flexible material of natural rubber, silicone resin, or thermoplastic elastomer. If desired, the surface of the molded article of the above-described material is coated with ethylene trifluoride or tetrafluoroethylene to enhance the chemical resistance of the cap 32.
  • the cap 32 has an open cylindrical shape and has a cap bottom 34.
  • the lower end portion of the cap 32 is provided with a conical surface 35 in order to facilitate the fitting into the well 12.
  • the cap bottom portion 34 has a V-shaped groove 36 for thinning, and a needle for taking out the liquid contained in the well 12 can be inserted into the V-shaped groove 36.
  • the outer surface of the cap 32 is provided with four vertical grooves 40 at intervals of 90 degrees.
  • the cross section of the vertical groove 40 is semicircular.
  • the cross section of the vertical groove 40 may be trapezoidal, triangular, or the like.
  • the vertical groove 40 extends upward from the lower end.
  • the outer diameter of the cap 32 is 8.6 mm
  • the length (height) of the cap 32 is 2.2 mm
  • the length of the vertical groove 40 is 1.9 mm
  • the maximum length of the vertical groove 40 is The depth is 0.5 mm.
  • the number and position of the vertical grooves 40 are arbitrary, and for example, three vertical grooves can be arranged at equiangular intervals.
  • the cap plate member is arranged on the well member and pressing on the cap plate by the roller, the direction of pressing by the roller is arbitrary, so at least three longitudinal grooves are arranged at equiangular intervals. It is desirable.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne une plaque de puits munie de bouchons qui possède une configuration simple et qui peut être fermée par les bouchons, et pour laquelle il y a peu de possibilité que les éléments de plaques de bouchons se détachent de la plaque de puits une fois celle-ci fermée au moyen des éléments de plaque de bouchons. Cette plaque de puits munie de bouchons est formée par combinaison d'un élément de puits, qui est muni de multiples puits, et d'un élément de plaque de bouchons souple, qui possède de multiples bouchons qui sont agencés dans des positions correspondant aux positions de puits, et qui ferment individuellement les puits respectifs, et la plaque de puits munie de bouchons est caractérisée en ce que le diamètre intérieur d'un puits et le diamètre extérieur d'un capuchon sont formés de telle sorte que le diamètre intérieur de puits est inférieur au diamètre extérieur du capuchon, de sorte que le capuchon puisse être agencé par pression sur le puits ; les éléments de capuchon sont formés sous la forme d'un corps par reliure des multiples bouchons au moyen d'une partie de plaque ; et les bouchons sont formés pour s'ouvrir au niveau de la partie supérieure afin de permettre à une aiguille d'être insérée dans la partie inférieure, et pour posséder sur la surface extérieure des rainures verticales qui s'étendent depuis les bords inférieurs vers un point médian dans la direction de hauteur.
PCT/JP2009/069438 2008-11-14 2009-11-16 Plaque de puits munie de bouchons WO2010055939A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2008-292109 2008-11-14
JP2008292109A JP2010117305A (ja) 2008-11-14 2008-11-14 キャップ付きウエルプレート

Publications (1)

Publication Number Publication Date
WO2010055939A1 true WO2010055939A1 (fr) 2010-05-20

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ID=42170068

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2009/069438 WO2010055939A1 (fr) 2008-11-14 2009-11-16 Plaque de puits munie de bouchons

Country Status (2)

Country Link
JP (1) JP2010117305A (fr)
WO (1) WO2010055939A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110124764A (zh) * 2019-05-30 2019-08-16 天津国科医工科技发展有限公司 用于全自动液体处理的试剂挥发抑制装置及其抑制方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI498167B (zh) * 2013-07-03 2015-09-01 Wistron Corp 用來導引分注器吸取孔盤所容置溶液之導引裝置

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4996355U (fr) * 1972-12-09 1974-08-20
JPH06324052A (ja) * 1993-05-12 1994-11-25 Hitachi Ltd サンプルカップおよびオートサンプラならびにオートサンプラ付分析装置
JP2001281113A (ja) * 2000-03-31 2001-10-10 Shino Test:Kk 蓋体付き容器
JP2002159284A (ja) * 2000-11-27 2002-06-04 Sumitomo Bakelite Co Ltd 試料保存用マルチウェルプレート
JP2004359286A (ja) * 2003-06-04 2004-12-24 Toho Kogyo Kk 蓋部材装着装置

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4996355U (fr) * 1972-12-09 1974-08-20
JPH06324052A (ja) * 1993-05-12 1994-11-25 Hitachi Ltd サンプルカップおよびオートサンプラならびにオートサンプラ付分析装置
JP2001281113A (ja) * 2000-03-31 2001-10-10 Shino Test:Kk 蓋体付き容器
JP2002159284A (ja) * 2000-11-27 2002-06-04 Sumitomo Bakelite Co Ltd 試料保存用マルチウェルプレート
JP2004359286A (ja) * 2003-06-04 2004-12-24 Toho Kogyo Kk 蓋部材装着装置

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MODULE PLATE, SHINWA FRESH FLASH INFORMATION, vol. 36, August 1999 (1999-08-01) *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110124764A (zh) * 2019-05-30 2019-08-16 天津国科医工科技发展有限公司 用于全自动液体处理的试剂挥发抑制装置及其抑制方法

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