WO2010047533A2 - Activateur de la pousse de cheveux obtenu par un procédé de nano-extraction à basse température, et ce procédé de fabrication - Google Patents

Activateur de la pousse de cheveux obtenu par un procédé de nano-extraction à basse température, et ce procédé de fabrication Download PDF

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WO2010047533A2
WO2010047533A2 PCT/KR2009/006099 KR2009006099W WO2010047533A2 WO 2010047533 A2 WO2010047533 A2 WO 2010047533A2 KR 2009006099 W KR2009006099 W KR 2009006099W WO 2010047533 A2 WO2010047533 A2 WO 2010047533A2
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extract
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weight
parts
hair growth
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WO2010047533A3 (fr
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박영호
김준한
박진홍
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(주)제이더블유바이오
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/673—Vitamin B group
    • A61K8/675—Vitamin B3 or vitamin B3 active, e.g. nicotinamide, nicotinic acid, nicotinyl aldehyde
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/368—Carboxylic acids; Salts or anhydrides thereof with carboxyl groups directly bound to carbon atoms of aromatic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
    • A61K8/4926—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having six membered rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706—Algae
    • A61K8/9711—Phaeophycota or Phaeophyta [brown algae], e.g. Fucus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9728—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9755—Gymnosperms [Coniferophyta]
    • A61K8/9761—Cupressaceae [Cypress family], e.g. juniper or cypress
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9755—Gymnosperms [Coniferophyta]
    • A61K8/9767—Pinaceae [Pine family], e.g. pine or cedar
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/12—Preparations containing hair conditioners
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/41—Particular ingredients further characterized by their size
    • A61K2800/413—Nanosized, i.e. having sizes below 100 nm

Definitions

  • the present invention relates to a hair growth promoter using a low temperature nano-extraction method and a manufacturing method thereof, and more specifically, the hair growth promoting agent using the low-temperature nano extraction method extracted with a nano-size of 1000 ⁇ 3000 ⁇ at 15 ⁇ 60 °C using an organic solvent.
  • the present invention relates to a hair growth promoting agent using a low-temperature nano-extraction method, including amide, salicylic acid, Bongyeong extract, lettuce extract, jinjin mugwort extract, seaweed extract, and kelp extract.
  • Human hair has a cosmetic function in addition to functional aspects such as protecting the head from sunlight, absorbing shock from external shock, and maintaining body temperature.
  • the number of female hair loss patients has increased due to changes in lifestyles such as westernized eating habits and frequent hair pollution such as perm and dyeing, and adolescent hair loss due to the fact that smoking, academic stress, and puberty itself are accelerated by an average of three years. Patients are also increasing.
  • Alopecia is a disease that is lost in the area where hair is normally present. It occurs mainly in the head and is classified into non-alopecia areata and alopecia areata according to the damage of hair follicles. No specific drug has been found. Minoxidil has been developed and used as an antihypertensive agent, and since 1977 Dargie et al. First reported the occurrence of hirsutism after oral administration of minoxidil, the topical coating of minoxidil began to be used as a treatment for alopecia.
  • an object of the present invention is a hair growth accelerator using a low-temperature nano extraction method that solves the problems of side effects caused by conventional unchecked hair loss preventing agent and hair growth promoter by manufacturing a hair growth promoter extracted from natural herbs and seaweeds at low temperature nanoscale And the purpose is to provide a method for producing the same.
  • the present invention as a hair growth promoter, nicotinic acid amide, salicylic acid, bongyeong extract, lettuce extract, jinjin mugwort extract, seaweed extract, and kelp extracted at a nano size at 15 ⁇ 60 °C using an organic solvent It provides a hair growth promoting agent using a low-temperature nano extraction method comprising an extract.
  • the present invention is a method for producing a hair growth promoter, the main solution (A) in which nicotinic acid amide is added to ethanol, and benzoic acid and lavender oil are sequentially added to salicylic acid to dissolve the ethanol, purified water, and seaweed in seaweed, kelp, pine needles, Boiled with baekbaek leaf, black soybean, woobangeun, black jima, sewage, Cheongung, Bongyeong, Sangbaekpi, Injin mugwort, and Chihwang, and extracted with 20 ⁇ 50% alcoholic water as an extraction solvent at 15 ⁇ 60 °C for 24 ⁇ 48 hours.
  • the resultant was filtered with an ultrafilter, again filtered with a nanofilter, separated by centrifugation, placed in a vacuum depressurizer, and concentrated at a temperature of 30-50 ° C. for 1-5 hours to evaporate ethanol. After the addition, and then added to the extraction solution (B) to lower the alcohol concentration to 0.5 to 5%, to provide a method for producing a hair growth promoter using a low-temperature nano extraction method comprising obtaining a uniform solution of greenish brown.
  • the hair growth promoter using the cold nano extraction method of the present invention and a method for producing the same are used for the low temperature nano extraction method, so that the nano-sized hair growth promoter is well absorbed into the hair follicles, thereby inhibiting the expression of TGF- ⁇ 2, a hair growth cell degenerative protein, hair loss.
  • TGF- ⁇ 2 a hair growth cell degenerative protein, hair loss.
  • herbal medicines and seaweeds are used as raw materials, various side effects of hormone therapy are prevented and shedding phenomenon is overcome.
  • it contributes to the creation of employment and the local economy, as well as the vitalization of R & D in the bio industry, which can replace the local industry, and the effect of replacing hair loss, wool, hair growth, and hair growth-related products.
  • FIG. 1 is a process chart showing a method for preparing hair growth promoter using a low temperature nano-extraction method of the present invention.
  • Figure 2 is a process chart showing the technical standards of cold nano extraction, separation and concentration products of the present invention.
  • Figure 3 is a graph showing the change in organic acid according to the storage period of the extract of the present invention.
  • Figure 4 is a graph showing the change in phenolic acid according to the storage period of the extract of the present invention.
  • Figure 5 is a graph measuring the proliferation effect of HHDPC hair-forming cells by the hair growth promoter JW12 of the present invention.
  • Figure 6 is an electrophoresis picture showing the suppression of the expression of TGF- ⁇ 2 hair growth cell induction phase in HaCaT hair-forming cells by the hair growth promoter JW12 of the present invention.
  • Figure 7 is an electrophoresis picture showing the suppression of the expression of hair growth cell degeneration phase protein TGF- ⁇ 2 in HHDPC hair-forming cells by the hair growth promoter JW12 of the present invention.
  • FIG. 8 is a photograph showing the regeneration of the hair growth cycle by the hair promoter JW12 of the present invention.
  • FIG. 9 is an animal photograph showing the hair growth promoting effect of the C57BL / 6 mouse model by the hair growth promoter JW12 of the present invention.
  • 10 to 16 are hair pictures of subjects whose hair growth is promoted through the hair loss prevention program of the present invention.
  • the present invention is a hair growth promoting agent using a low-temperature nano-extraction method, nicotinic acid amide, salicylic acid, bongyeong extract, lettuce extract, jinjin mugwort extract, seaweed extract, and kelp extract extracted at a nano size of 1000 ⁇ 3000 ⁇ at 15 ⁇ 60 °C using an organic solvent It includes.
  • the hair growth promoting agent using the low temperature nano-extraction method further includes pine needle extract, cypress leaf extract, black soybean extract, and right root extract.
  • the hair growth promoting agent using the low-temperature nano extraction method further includes black jima extract, sewage extract, cheongung extract, and goji extract.
  • the organic solvent includes ethanol or ether.
  • the hair growth promoting agent using the low temperature nano-extraction method is added to the nicotinic acid amide to ethanol, benzoic acid as a preservative and lavender oil as a flavoring agent to the salicylic acid in order to dissolve the main solution (A) is the ethanol, the purified water, And seaweed, seaweed, pine needles, pine needles, cypress leaves, black soybeans, woobangeun, black jima, sewage, cheongung, bongyeong, baekbaekpi, injin mugwort and jihwang after boiling with 30% alcoholic water as an extraction solvent, and less than 3%
  • the alcohol concentration to the extraction solution (B) was added to make a uniform solution and then separated by centrifugation, filtered by nanometer, sterilized and put in a container.
  • the size of each functional extract is a nano size of 1000 ⁇ 3000 ⁇ .
  • the hair growth promoter is well absorbed by hair follicles and inhibits the expression of TGF- ⁇ 2, a hair growth cell degenerative protein, to prevent hair loss, and is useful for wool, hair growth, and hair growth.
  • the nano-sized hair growth promoter is well absorbed by hair follicles and is useful for hair loss prevention, wool, hair growth, and hair growth.
  • the hair growth promoter using the low temperature nano-extraction method overcomes the shedding phenomenon. Shading is a phenomenon in which newly growing healthy hair pushes out resting hair which should be removed anyway, which is a normal process that occurs between two to six weeks after hair loss treatment, and hair falls out more than usual at this time.
  • the nicotinic acid amide is one of the water-soluble vitamin B complexes, like nicotinic acid, and exists in vivo as nicotinamide nucleotides that are coenzymes of this vitamin, namely NAD + and NADP +, and is involved in many oxidation and reduction reactions as coenzymes of dehydrogenase.
  • Another function of nicotinamide nucleotides is the reduction of flavincoenzyme, in which case proton addition or desorption is involved in the 4-membered pyridine ring carbon atom.
  • the nicotinic acid amide is hydrolyzed by nicotinamidase and the resulting nicotinic acid is reused for NAD synthesis.
  • the nicotinic acid amide is methylated in the liver to form n-methylnicotinic acid amide, which is mixed with urine and discharged.
  • salicylic acid Since salicylic acid has the action of antipyretic and analgesic as o-oxybenzoic acid, it is internalized with sodium salt or calcium salt, and is used to improve solubility, stability, pharmacology, etc. by combining salicylic acid with various drugs.
  • the order is to protect the intestines and dried the fungal nucleus of Bokryeong, diuretic and lowering blood sugar, sedation and the like.
  • the baekbaekpi is a mulberry bark anti-inflammatory action, antioxidant action, skin soothing action, to prevent aging.
  • the jinjin mugwort is called cheonjae wormwood or Injincho and jaundice, soy sauce, cold cold, anti-inflammatory diuretics and the like to take.
  • the phosphorus mugwort contains dimethylescholetin, scopoline, essential oils, aromatic oxycarboxylic acids, flavonoids, oxycoumarins and the like in the outpost.
  • the seaweed is rich in dietary fiber and potassium, calcium, iodine, etc., to promote metabolism, postpartum cooking, constipation, obesity prevention, iron and calcium supplements are excellent.
  • the kelp contains a lot of carbohydrates, such as manit and laminarin, which are made by carbon assimilation, and alginic acid, which are components of cell walls, and iodine, vitamin B2, glutamic acid, and the like. Contains amino acids The kelp contains laminin, which lowers blood pressure.
  • the pine needles are an ingredient of oxyl palmitic acid is effective for skin, beauty and heart strengthening.
  • the white leaf has a hemostatic action.
  • the right root is the root of burdock soothes the sore face and treats sore throat, boil, mange and ringworm. It has been reported that pharmacological action also acts against the various filamentous fungi that cause cancer, activate liver function, purulent bacteria and skin disease.
  • the black jima is a black sesame to promote the recovery of the skin mucous membranes, greatly reduce the cholesterol level in the blood, and activate the bowel movements.
  • the sewage is tonic action, hematopoietic function strengthening, fatigue recovery action.
  • the uterus has a sedation, blood pressure lowering action, antibacterial action.
  • the sulfur is lowering blood sugar, diuretic, antibacterial action.
  • the content of the nicotinic acid amide of the hair growth promoter is 0.03 to 5.0 parts by weight
  • the content of the salicylic acid is 0.01 to 5 parts by weight
  • the content of the Bongyeong extract is 0.05 to 10 parts by weight
  • the content of the lettuce extract is 0.03 to 8 parts by weight.
  • the content of the jinjinjin mugwort extract is 0.05 to 10 parts by weight
  • the content of the seaweed extract is 0.5 to 30 parts by weight
  • the content of the kelp extract is 0.5 to 30 parts by weight.
  • the content of the pine needle extract of the hair growth promoter is 0.5 to 10 parts by weight
  • the content of the cypress leaf extract is 0.5 to 10 parts by weight
  • the content of the black soybean extract is 0.05 to 8 parts by weight
  • the content of woobangeun extract 0.03 to 5 parts by weight.
  • the content of the black jima extract of the hair growth promoter is 0.05 to 8 parts by weight
  • the content of the sewage extract is 0.03 to 5 parts by weight
  • the content of the cheongung extract is 0.03 to 5 parts by weight
  • the content of the sulfuric acid extract is 0.03-7 weight part.
  • a method for preparing a hair growth promoting agent using the low-temperature nano extraction method includes adding nicotinic acid amide to ethanol, and sequentially adding benzoic acid and lavender oil to salicylic acid to dissolve the main solution (A) into the ethanol, purified water, and seawater. Boil it with kelp, pine needles, cypress leaves, black soybeans, right roots, black jima, sewage, Cheongung, Bongyeong, Sangbaekpi, Phosphorus mugwort and zucchini, and then extract with 20 ⁇ 50% alcohol water for 15 ⁇ 60 °C for 24 ⁇ 48 hours.
  • Each extract was prepared by extracting from the filtrate, followed by filtration with an ultrafilter, followed by filtration with a nanofilter, followed by separation through centrifugation, and then into a vacuum vacuum concentrator, followed by concentration at a temperature of 30 to 50 ° C. for 1 to 5 hours. After evaporation of ethanol, the resultant is added to the extraction solution (B) having the alcohol concentration lowered to 0.5-5%, to obtain a greenish brown uniform solution.
  • the content of the nicotinic acid amide is 0.03 to 5.0 parts by weight
  • the content of the salicylic acid is 0.01 to 5 parts by weight
  • the content of the seaweed extract is 0.5 to 30 parts by weight
  • the content of the kelp extract is 0.5 to 30 parts by weight
  • the content of pine needle extract is 0.5 to 10 parts by weight
  • the content of the cypress leaf extract is 0.5 to 10 parts by weight
  • the content of the black soybean extract is 0.05 to 8 parts by weight
  • the content of the right root extract is 0.03 to 5 parts by weight
  • the content of the black jima extract is 0.05 to 8 parts by weight
  • the content of the sewage extract is 0.03 to 5 parts by weight
  • the content of the cheongung extract is 0.03 to 5 parts by weight
  • the content of the extract is 0.05 to 10 parts by weight
  • the content of the turmeric extract is 0.03 to 7 parts by weight.
  • the method for preparing a hair growth promoter using the low temperature nano extraction method is to perform the sorting, washing, drying, or shredding of the raw materials as preparation before extraction.
  • the method for producing hair growth promoter using the low temperature nano extraction method is to extract the raw material by applying a step low temperature nano extraction method to the target object using ultrasonic waves after the screening and washing process using natural herbal ingredients and seaweeds to prevent hair loss prevention products It is intended to be manufactured in a way that maximizes the effective and extraction of specific components.
  • FIG. 1 is a process chart showing a method for preparing hair growth promoter using a low temperature nano-extraction method of the present invention.
  • the preparation work before extraction such as sorting, washing, drying, crushing.
  • each raw material is extracted with an organic solvent containing ethanol and distilled water.
  • the ultrafiltration process is performed, and the extract which is difficult to be separated by normal filtration is filtered by using dialysis paper using a sulfate sheet, a bladder membrane, and colloidion.
  • the extraction solution (B) is added with the nicotinic acid amide to the ethanol, and the benzoic acid and the lavender oil are sequentially added to the salicylic acid and added to the dissolved main solution (A). Produces a solution, a product.
  • the hair loss management system of the present invention consultation and shooting; Primary sampu; Scalp steamer; Scalp peeling and oxygen supply; Infrared radiation; Ampoule application; Scalp massage; And secondary sampu; sequentially.
  • a person consults about eating habits and hair hygiene, and photographs a bald area with a digital camera. At this time, shoot with the hair enlarger.
  • the scalp steamer step facilitates the removal of fine contaminants remaining in the scalp (mogu) by steam and absorption of the hair growth promoter ampoule.
  • oxygen is supplied to the scalp and the contaminant filling and scalp such as foreign matter in the scalp (mogu) relaxed by steam.
  • infrared irradiation step helps to circulate blood using infrared rays.
  • the hair growth promoter ampoule for nourishing the scalp is finely sprayed with an oxygen jet spray.
  • the final shampoo after absorbing the hair growth promoter ampoule creates a personal style.
  • each material Natural herbal medicine and algae
  • it is prepared by washing, drying, crushing, etc. for detailed selective extraction according to the functional material of each raw material. It was made to include the extract.
  • the extracted active ingredients are again subjected to primary extraction and concentration using ultrafiltration (ultrafiltration, MWCO, 20,000), followed by extraction and concentration according to molecular weight in the second order through nanofiltration (nanofiltration, MWCO, 10,000).
  • MWCO ultrafiltration
  • nanofiltration nanofiltration
  • composition and content of the extract in 100 ml of hair growth promoter (product name: JW12) using the low temperature nano extraction method of the present invention are as follows.
  • the content of nicotinic acid amide is 0.1g
  • the content of salicylic acid is 0.1g
  • the content of seaweed extract is 3g
  • the content of seaweed extract is 4g
  • the content of pine needle extract is 2.5g
  • the content of cypress leaf extract is 2.5g
  • the content of Insam mugwort extract is 0.5g
  • the content of the sulfuric acid extract contains 0.3g, 0.2g of benzoic acid as a preservative, 0.1g of lavender oil as a flavoring agent, 30g of ethanol, and the rest is purified water and seawater Put it.
  • ICP Inductively Coupled Plasma Emission Spectrometer, Thermo Jarrel Ash Co., IRIS / AP
  • Sample (3 g) was added H 2 O 2 2 to HN 3 10 and digested with Microwave (Malstone Co.,) and filled up with standard amount 20 with 2-3% HNO 3 .
  • HPLC High Performance Liquid Chromatography, Waters Co., Sugarpak Icolumn
  • Sample 1 was filtered through a microfilter and used as the sample.
  • An automatic amino acid analyzer (Automatic Amio Acid Analyzer, Pharmacia Biotech Co., Biochrom 20, Na form column) was used to investigate the amino acid content and content of the sample.
  • the sample was hydrolyzed with 6N hydrochloric acid (HCl), filtered through a microfilter, and used as a sample.
  • HCl 6N hydrochloric acid
  • Vitamin component of the product JW12; hair growth promoter (unit: mg / 100g)
  • JW12 was divided into 1st (2007/02), 2nd (2007/04), 3rd (2007/06), and 4th (2007/08), and the change of organic acid and phenolic acid were tested at two-month intervals. Table 7, Figure 3; Table 8, Figure 4).
  • HHDPC Human dermal papilla cells
  • the final concentration of JW12, a natural product extract was used at a final concentration of 0.05%, 0.1%, and 0.5%, and H 2 O was used as a negative control.
  • Multi- [4,5-dimethylthiazol-2-yl] -2 and 5-diphenyltetraolium bromide test (MTT) method was used to quantify the formation of the formazan (formazan) pigment by viable mitochondria. was carried out.
  • the HHDPC cells were adjusted to an initial cell concentration of about 1 ⁇ 10 4 cells / well per well using a 96-hole plate incubator.
  • the medium was adjusted to an amount of about 200ml per well and incubated in 37 ° C. and 5% CO 2 incubator for 3 days.
  • the absorbance value was measured at 540 nm using an ELISA reader, and the cell number was measured according to a standard sample curve prepared in advance.
  • Human keratinocyte HaCaT was used as hair-forming cells, and cell culture medium was cultured using RPMI, and subcultured once every 3 days.
  • HHDPC Human dermal papilla cells
  • MSC Human dermal papilla cells
  • JW12 a natural product extract
  • a final concentration of 0.1% or 1% was used.
  • RNA Isolate total RNA from each cell by chromoform extraction and isopropanol precipitation method and quantify RNA.
  • TGF- ⁇ 2 cDNA Synthesize TGF- ⁇ 2 cDNA using reverse transcriptase from total RNA isolated using TGF- ⁇ 2 specific primer using human TGF- ⁇ 2 gene sequence and electrophores TGF- ⁇ 2 DNA generated by PCR method.
  • FIG. 6 (A) shows Total RNA from HaCaT treated with JW12;
  • Figure 6 (B) is RT-PCR products by specific primer for TGF- ⁇ 2.
  • Figure 7 (A) is Total RNA from HHDPC treated with JW12;
  • Figure 7 (B) is RT-PCR products by specific primer for TGF- ⁇ 2.
  • RT-PCR was performed using each group of RNA isolated from each group in HaCaT and HHDPC cells as TGF- ⁇ 2 primer. The results showed that the expression of TGF- ⁇ 2 was higher in both HaCaT and HHDPC cells than in the group treated with JW12.
  • the group treated with 0.1% JW had higher expression of TGF- ⁇ 2 than the group treated with 1% JW12.
  • JW12 has an effect of delaying the entry time of Anagen to Catagen in the hair cycle cycle (see FIG. 8).
  • 1 HE accelerated the hair growth cycle for about 2 days compared to the trypsin treatment group which delayed the hair growth phase.
  • HE treatment group promoted hair growth stage about 1 day than mice with normal hair growth cycle.
  • the HE-1 and HE-2 medicinal treatment groups showed about 11.4% more hair growth promoting effect than the hair growth delay group.
  • hair follicle cells were cultured in a culture medium containing JW12 in the cytotoxic effect on dermal papilla cells, which are hair-forming cells.
  • JW12 showed no toxicity in the hair follicle cell group with 0.05% and 0.1% added, and the cell growth rate of about 16% was observed in 0.05%.
  • TGF- ⁇ 2 gene expression a protein that induces GI, was isolated from total hair follicle cells treated with JW12, and RT-PCR was performed using a TGF- ⁇ 2 specific primer. In, it was observed that the expression level of TGF- ⁇ 2 was significantly decreased compared to the control group not treated with JW12.
  • 10 to 16 are hair pictures of subjects whose hair growth is promoted through the hair loss prevention program of the present invention. 10 to 16 subjects were successfully hair growth through the hair loss prevention program.
  • the present invention described above uses low-temperature nano-extraction, so that the nano-sized hair growth promoter is well absorbed by hair follicles and is useful for hair loss prevention, wool, hair growth, and hair growth, and prevents various side effects of hormonal therapeutic agents as raw materials of herbal medicine and seaweed And overcome the shedding phenomenon.
  • it contributes to the creation of employment and the local economy, as well as the vitalization of R & D in the bio industry, which can replace the local industry, and the effect of replacing hair loss, wool, hair growth, and hair growth-related products.

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Abstract

Cette invention concerne un activateur de la pousse des cheveux obtenu par un procédé de nano-extraction à basse température et son procédé de fabrication. Plus particulièrement, l’invention concerne un activateur de la pousse de cheveux fabriqué selon un procédé de nano-extraction à basse température, ledit activateur contenant un amide d’acide nicotinique, un acide salicylique, de l’extrait de cocos Poria, de l’extrait de Morus alba, de l’extrait d’Artemisia capillaries, de l’extrait de varech et de l’extrait de kombu, qui sont extraits à un niveau nanométrique de 1000~3000Å à une température comprise entre 15 et 60°C au moyen d’un solvant organique. L’invention concerne également un procédé de fabrication dudit activateur. Cet activateur obtenu par nano-extraction à basse température peut être facilement absorbé dans les follicules pileux et peut donc être utilisé pour prévenir la chute des cheveux ainsi que pour le traitement et la pousse des cheveux. Comme il est par ailleurs obtenu à partir de plantes médicinales et de varech, le dit activateur permet d’éviter divers effets secondaires provoqués par des agents d’hormonothérapie et de réduire la mue pilaire. De plus, cette invention peut stimuler la recherche et le développement  d’une bioindustrie venant se substituer à des industries locales, stimuler l’emploi et l’économie tout en s’affranchissant sensiblement de l’importation de produits en rapport avec la prévention de la chute des cheveux et avec le traitement et la pousse de cheveux.
PCT/KR2009/006099 2008-10-21 2009-10-21 Activateur de la pousse de cheveux obtenu par un procédé de nano-extraction à basse température, et ce procédé de fabrication WO2010047533A2 (fr)

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KR1020080103200A KR100908745B1 (ko) 2008-10-21 2008-10-21 저온나노추출법을 이용한 발모촉진제 및 그 제조방법
KR10-2008-0103200 2008-10-21

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WO2010047533A3 WO2010047533A3 (fr) 2010-08-19

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GB2461037A (en) * 2008-06-17 2009-12-23 Elias Bouras A method of treating alopecia using a composition comprising a cardiac glycoside
KR101110236B1 (ko) * 2009-07-13 2012-02-15 박영호 비타민 함유 저온나노추출 발모촉진제 및 그 제조방법
KR101121579B1 (ko) * 2009-07-13 2012-03-06 박영호 유기용매와 물의 혼합용매 또는 물을 사용하여 저온에서 나노추출한 발모촉진제 및 그 제조방법
KR101150373B1 (ko) 2009-07-13 2012-06-08 박영호 구리-펩타이드 함유 저온나노추출 발모촉진제 및 그 제조방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR970032802A (ko) * 1995-12-05 1997-07-22 양병일 발모촉진제 조성물
KR100202834B1 (ko) * 1997-05-31 1999-06-15 남휘추 탈모 예방 및 발모촉진 머릿기름의 제조방법
KR20020084718A (ko) * 2001-05-02 2002-11-11 박의석 발모촉진과 탈모 예방 및 두피 세정을 목적으로 하는 헤어토닉의 제조 방법
KR20070031978A (ko) * 2007-02-28 2007-03-20 (주)우솔홀딩스 발모 촉진용 조성물과 제조방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR970032802A (ko) * 1995-12-05 1997-07-22 양병일 발모촉진제 조성물
KR100202834B1 (ko) * 1997-05-31 1999-06-15 남휘추 탈모 예방 및 발모촉진 머릿기름의 제조방법
KR20020084718A (ko) * 2001-05-02 2002-11-11 박의석 발모촉진과 탈모 예방 및 두피 세정을 목적으로 하는 헤어토닉의 제조 방법
KR20070031978A (ko) * 2007-02-28 2007-03-20 (주)우솔홀딩스 발모 촉진용 조성물과 제조방법

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YONHAP NEWS PRESS INFORMATION 10 July 2006, *

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