WO2010039241A1 - Methods for isolating very small embryonic-like (vsel) stem cells - Google Patents

Methods for isolating very small embryonic-like (vsel) stem cells Download PDF

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Publication number
WO2010039241A1
WO2010039241A1 PCT/US2009/005414 US2009005414W WO2010039241A1 WO 2010039241 A1 WO2010039241 A1 WO 2010039241A1 US 2009005414 W US2009005414 W US 2009005414W WO 2010039241 A1 WO2010039241 A1 WO 2010039241A1
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WIPO (PCT)
Prior art keywords
cells
vsels
population
enriched
cell
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PCT/US2009/005414
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English (en)
French (fr)
Inventor
Ewa K. Zuba-Surma
Mariusz Ratajczak
Janina Ratajczak
Magdalena Kucia
Original Assignee
University Of Louisville Research Foundation, Inc.
Neostem, Inc.
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Publication date
Application filed by University Of Louisville Research Foundation, Inc., Neostem, Inc. filed Critical University Of Louisville Research Foundation, Inc.
Priority to CN2009801481025A priority Critical patent/CN102333861A/zh
Priority to US13/121,913 priority patent/US20120021482A1/en
Publication of WO2010039241A1 publication Critical patent/WO2010039241A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
PCT/US2009/005414 2008-09-30 2009-09-30 Methods for isolating very small embryonic-like (vsel) stem cells WO2010039241A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN2009801481025A CN102333861A (zh) 2008-09-30 2009-09-30 用于分离非常小的胚胎样(vsel)干细胞的方法
US13/121,913 US20120021482A1 (en) 2008-09-30 2009-09-30 Methods for isolating very small embryonic-like (vsel) stem cells

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US19471908P 2008-09-30 2008-09-30
US61/194,719 2008-09-30
US19892008P 2008-11-12 2008-11-12
US61/198,920 2008-11-12
US24565009P 2009-09-24 2009-09-24
US61/245,650 2009-09-24

Publications (1)

Publication Number Publication Date
WO2010039241A1 true WO2010039241A1 (en) 2010-04-08

Family

ID=42073776

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/005414 WO2010039241A1 (en) 2008-09-30 2009-09-30 Methods for isolating very small embryonic-like (vsel) stem cells

Country Status (3)

Country Link
US (1) US20120021482A1 (zh)
CN (1) CN102333861A (zh)
WO (1) WO2010039241A1 (zh)

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101948802A (zh) * 2010-08-31 2011-01-19 中国人民解放军第三军医大学第一附属医院 免疫磁珠分选人关节软骨cd105+/cd166+间充质干细胞的方法
CN102373176A (zh) * 2010-08-23 2012-03-14 杨跃进 一种极小类胚胎样干细胞的制备方法
WO2012019002A3 (en) * 2010-08-04 2012-05-03 StemBios Technologies, Inc. Somatic stem cells
WO2013049459A3 (en) * 2011-09-28 2013-06-06 StemBios Technologies, Inc. Somatic stem cells
WO2013181642A1 (en) * 2012-05-31 2013-12-05 Neostem, Inc. Human very small embryonic-like (vsel) stem cells for treatment of ocular disease
WO2014004409A2 (en) * 2012-06-24 2014-01-03 Neostem, Inc Stem cell treatment for radiation exposure
EP2694641A1 (en) * 2011-04-08 2014-02-12 The Regents Of The University Of Michigan Autologous human adult pluripotent very small embryonic-like (hvsel) stem cell regeneration of bone and cartilage
JP2014073084A (ja) * 2012-10-02 2014-04-24 Kansai Medical Univ 微小幹細胞の分離方法
WO2014125277A1 (en) 2013-02-12 2014-08-21 Reneuron Limited Method of producing microparticles
US9415073B2 (en) 2010-08-04 2016-08-16 StemBios Technologies, Inc. Somatic stem cells
US9770473B2 (en) 2009-02-24 2017-09-26 StemBios Technologies, Inc. Treatment of immunosuppression-related disorders
US9810684B2 (en) 2012-12-21 2017-11-07 StemBios Technologies, Inc. Method for increasing number of stem cells in human or animal bodies
US9856452B2 (en) 2012-12-06 2018-01-02 StemBios Technologies, Inc. Lgr5+ somatic stem cells
US9987310B2 (en) 2013-11-27 2018-06-05 University Of Louisville Research Foundation, Inc. Cardiac progenitor cells and methods of use therefor
EP3220929A4 (en) * 2014-11-19 2018-06-27 Stembios Technologies, Inc. Somatic stem cells for treating bone defects
EP3370740A4 (en) * 2015-11-04 2019-05-22 The Board of Regents of The University of Texas System ENRICHMENT AND REINFORCEMENT OF HIGH POTENTIAL HUMAN MESENCHYMAL STEM CELLS FROM OLD CELL POPULATIONS
US11072777B2 (en) 2016-03-04 2021-07-27 University Of Louisville Research Foundation, Inc. Methods and compositions for ex vivo expansion of very small embryonic-like stem cells (VSELs)
US11312940B2 (en) 2015-08-31 2022-04-26 University Of Louisville Research Foundation, Inc. Progenitor cells and methods for preparing and using the same

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012174225A2 (en) 2011-06-14 2012-12-20 The University Of North Carolina At Chapel Hill Isolation, expansion and use of autologous pluripotent stem cells
TW201433296A (zh) * 2013-02-21 2014-09-01 Univ Nat Taiwan 一種血球之觀察鑑測方法
CA2915540C (en) * 2013-03-14 2021-08-31 Irvine Scientific Sales Company, Inc. A method and quality control molecular based mouse embryo assay for use with in vitro fertilization technology
EP3068872B1 (en) * 2013-11-14 2020-04-01 The University of Miami Non-expanded post-natal multilineage-inducible cells
JP2016080563A (ja) 2014-10-20 2016-05-16 日本光電工業株式会社 分析システム、分析装置

Citations (2)

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Publication number Priority date Publication date Assignee Title
US20050255588A1 (en) * 1999-09-24 2005-11-17 Young Henry E Pluripotent embryonic-like stem cells, compositions, methods and uses thereof
WO2007067280A2 (en) * 2005-12-08 2007-06-14 University Of Louisville Research Foundation, Inc. Very small embryonic-like (vsel) stem cells and methods of isolating and using the same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050255588A1 (en) * 1999-09-24 2005-11-17 Young Henry E Pluripotent embryonic-like stem cells, compositions, methods and uses thereof
WO2007067280A2 (en) * 2005-12-08 2007-06-14 University Of Louisville Research Foundation, Inc. Very small embryonic-like (vsel) stem cells and methods of isolating and using the same

Non-Patent Citations (2)

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Title
"BD Pharm Lyse 555899 - MSDS.", BD BIOSCIENCES, 2006, Retrieved from the Internet <URL:http://www.bdbiosciences.com/externaltiles/pm/doclmsds/livelwebenabled/555899_MSDS_US_EN.02.pdf> [retrieved on 20100208] *
RATAJCZAK ET AL.: "Very Small Embryonic-Like (VSEL) Stem Cells: Purification from Adult Organs", CHARACTERIZATION, AND BIOLOGICAL SIGNIFICANCE. STEM CELL RESEARCH, vol. 4, May 2008 (2008-05-01), pages 89 - 99 *

Cited By (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9770473B2 (en) 2009-02-24 2017-09-26 StemBios Technologies, Inc. Treatment of immunosuppression-related disorders
TWI686202B (zh) * 2010-08-04 2020-03-01 幹細胞生物科技公司 體幹細胞
CN103097519A (zh) * 2010-08-04 2013-05-08 干细胞生物科技公司 体干细胞
CN106148274A (zh) * 2010-08-04 2016-11-23 干细胞生物科技公司 体干细胞
TWI635177B (zh) * 2010-08-04 2018-09-11 幹細胞生物科技公司 體幹細胞
JP2013535215A (ja) * 2010-08-04 2013-09-12 ステムバイオス テクノロジーズ，インコーポレイテッド 体性幹細胞
JP2019010121A (ja) * 2010-08-04 2019-01-24 ステムバイオス テクノロジーズ，インコーポレイテッド 体性幹細胞
US10143710B2 (en) 2010-08-04 2018-12-04 StemBios Technologies, Inc. Somatic stem cells
TWI465569B (zh) * 2010-08-04 2014-12-21 Stembios Technologies Inc 體幹細胞
CN103097519B (zh) * 2010-08-04 2016-08-03 干细胞生物科技公司 体干细胞
WO2012019002A3 (en) * 2010-08-04 2012-05-03 StemBios Technologies, Inc. Somatic stem cells
US9415073B2 (en) 2010-08-04 2016-08-16 StemBios Technologies, Inc. Somatic stem cells
JP2017046702A (ja) * 2010-08-04 2017-03-09 ステムバイオス テクノロジーズ，インコーポレイテッド 体性幹細胞
TWI596211B (zh) * 2010-08-04 2017-08-21 幹細胞生物科技公司 體幹細胞
CN102373176A (zh) * 2010-08-23 2012-03-14 杨跃进 一种极小类胚胎样干细胞的制备方法
CN101948802A (zh) * 2010-08-31 2011-01-19 中国人民解放军第三军医大学第一附属医院 免疫磁珠分选人关节软骨cd105+/cd166+间充质干细胞的方法
EP2694641A4 (en) * 2011-04-08 2014-11-19 Univ Michigan REGENERATION OF VERY SMALL HUMAN EMBRYONIC STRAIN CELLS (HVSEL) PLURIPOTENT ADULTS AUTOLOGUES OF BONE AND CARTILAGE
EP2694641A1 (en) * 2011-04-08 2014-02-12 The Regents Of The University Of Michigan Autologous human adult pluripotent very small embryonic-like (hvsel) stem cell regeneration of bone and cartilage
CN103748215A (zh) * 2011-04-08 2014-04-23 密执安大学评议会 骨和软骨的自体人成熟多能性极小胚胎样(hvsel)干细胞的细胞再生
CN108611318A (zh) * 2011-09-28 2018-10-02 干细胞生物科技公司 成体干细胞
JP2014524258A (ja) * 2011-09-28 2014-09-22 ステムバイオス テクノロジーズ，インコーポレイテッド 体性幹細胞
WO2013049459A3 (en) * 2011-09-28 2013-06-06 StemBios Technologies, Inc. Somatic stem cells
CN103827295A (zh) * 2011-09-28 2014-05-28 干细胞生物科技公司 成体干细胞
US9777259B2 (en) 2011-09-28 2017-10-03 StemBios Technologies, Inc. Somatic stem cells
JP2017225450A (ja) * 2011-09-28 2017-12-28 ステムバイオス テクノロジーズ，インコーポレイテッド 体性幹細胞
JP2018198610A (ja) * 2011-09-28 2018-12-20 ステムバイオス テクノロジーズ，インコーポレイテッド 体性幹細胞
TWI614340B (zh) * 2011-09-28 2018-02-11 幹細胞生物科技股份有限公司 體幹細胞及其製備方法
CN103827295B (zh) * 2011-09-28 2018-03-06 干细胞生物科技公司 成体干细胞
US20160022743A1 (en) * 2012-05-31 2016-01-28 The Schepens Eye Research Institute, Inc. Human very small embryonic-like (vsel) stem cells for treatment of ocular disease
WO2013181642A1 (en) * 2012-05-31 2013-12-05 Neostem, Inc. Human very small embryonic-like (vsel) stem cells for treatment of ocular disease
WO2014004409A2 (en) * 2012-06-24 2014-01-03 Neostem, Inc Stem cell treatment for radiation exposure
WO2014004409A3 (en) * 2012-06-24 2014-02-20 Neostem, Inc Stem cell treatment for radiation exposure
JP2014073084A (ja) * 2012-10-02 2014-04-24 Kansai Medical Univ 微小幹細胞の分離方法
US9856452B2 (en) 2012-12-06 2018-01-02 StemBios Technologies, Inc. Lgr5+ somatic stem cells
US11492592B2 (en) 2012-12-06 2022-11-08 StemBios Technologies, Inc. Lgr5+ somatic stem cells
US9810684B2 (en) 2012-12-21 2017-11-07 StemBios Technologies, Inc. Method for increasing number of stem cells in human or animal bodies
WO2014125277A1 (en) 2013-02-12 2014-08-21 Reneuron Limited Method of producing microparticles
US9987310B2 (en) 2013-11-27 2018-06-05 University Of Louisville Research Foundation, Inc. Cardiac progenitor cells and methods of use therefor
EP3220929A4 (en) * 2014-11-19 2018-06-27 Stembios Technologies, Inc. Somatic stem cells for treating bone defects
US20190105353A1 (en) * 2014-11-19 2019-04-11 StemBios Technologies, Inc. Somatic stem cells for treating bone defects
US11312940B2 (en) 2015-08-31 2022-04-26 University Of Louisville Research Foundation, Inc. Progenitor cells and methods for preparing and using the same
EP3370740A4 (en) * 2015-11-04 2019-05-22 The Board of Regents of The University of Texas System ENRICHMENT AND REINFORCEMENT OF HIGH POTENTIAL HUMAN MESENCHYMAL STEM CELLS FROM OLD CELL POPULATIONS
US11802272B2 (en) 2015-11-04 2023-10-31 The Board Of Regents Of The University Of Texas System Enrich and amplify highly potent human mesenchymal stem cells from elderly cell populations
US11072777B2 (en) 2016-03-04 2021-07-27 University Of Louisville Research Foundation, Inc. Methods and compositions for ex vivo expansion of very small embryonic-like stem cells (VSELs)

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Publication number Publication date
US20120021482A1 (en) 2012-01-26
CN102333861A (zh) 2012-01-25

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