WO2010039180A3 - Détermination ultra-sensible de la concentration de molécules ou de particules d'analyte dans un échantillon fluidique - Google Patents

Détermination ultra-sensible de la concentration de molécules ou de particules d'analyte dans un échantillon fluidique Download PDF

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Publication number
WO2010039180A3
WO2010039180A3 PCT/US2009/005250 US2009005250W WO2010039180A3 WO 2010039180 A3 WO2010039180 A3 WO 2010039180A3 US 2009005250 W US2009005250 W US 2009005250W WO 2010039180 A3 WO2010039180 A3 WO 2010039180A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte molecules
substrate
particles
fluid sample
labeling agent
Prior art date
Application number
PCT/US2009/005250
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English (en)
Other versions
WO2010039180A2 (fr
Inventor
David C. Duffy
David M. Rissin
Linan Song
David R. Walt
Original Assignee
Quanterix Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quanterix Corporation filed Critical Quanterix Corporation
Priority to EP09736701A priority Critical patent/EP2352842A2/fr
Publication of WO2010039180A2 publication Critical patent/WO2010039180A2/fr
Publication of WO2010039180A3 publication Critical patent/WO2010039180A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing

Abstract

L'invention concerne des procédés, des systèmes et des trousses pour détecter, quantifier et/ou analyser un échantillon fluidique comprenant des molécules ou des particules d'analyte en faible concentration. Dans certains modes de la réalisation de l'invention, les procédés pour détecter et/ou pour quantifier les molécules d'analyte dans un échantillon consistent à capturer plusieurs molécules d'analyte sur un substrat (par exemple, un réseau comprenant plusieurs contenants de réaction). Le substrat peut ensuite être exposé à des composés réactionnels supplémentaires comme au moins un ligand de liaison. Le substrat peut éventuellement être également exposé à une molécule d'agent de marquage précurseur, cette molécule étant, dans certains cas, convertie en une molécule d'agent de marquage qui peut être détectée soit directement, soit indirectement, cette détermination pouvant être liée à la présence des molécules d'analyte et/ou pouvant être utilisée pour quantifier lesdites molécules d'analyte. Bien que des aspects variés de l'invention peuvent faire intervenir un certain nombre de formats d'épreuves différents, dans un mode de réalisation, les épreuves biologiques sont réalisées dans plusieurs contenants de réaction définis, au moins en partie, par les extrémités distales de brins de fibre optique.
PCT/US2009/005250 2008-09-23 2009-09-22 Détermination ultra-sensible de la concentration de molécules ou de particules d'analyte dans un échantillon fluidique WO2010039180A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP09736701A EP2352842A2 (fr) 2008-09-23 2009-09-22 Détermination ultra-sensible de la concentration de molécules ou de particules d'analyte dans un échantillon fluidique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/236,484 2008-09-23
US12/236,484 US20100075862A1 (en) 2008-09-23 2008-09-23 High sensitivity determination of the concentration of analyte molecules or particles in a fluid sample

Publications (2)

Publication Number Publication Date
WO2010039180A2 WO2010039180A2 (fr) 2010-04-08
WO2010039180A3 true WO2010039180A3 (fr) 2010-05-27

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PCT/US2009/005250 WO2010039180A2 (fr) 2008-09-23 2009-09-22 Détermination ultra-sensible de la concentration de molécules ou de particules d'analyte dans un échantillon fluidique

Country Status (3)

Country Link
US (1) US20100075862A1 (fr)
EP (1) EP2352842A2 (fr)
WO (1) WO2010039180A2 (fr)

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WO2010039180A2 (fr) 2010-04-08
US20100075862A1 (en) 2010-03-25
EP2352842A2 (fr) 2011-08-10

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