WO2010025374A3 - Manipulation de l’activité de la protéine kinase snf1 pour modifier la teneur en huile des organismes oléagineux - Google Patents

Manipulation de l’activité de la protéine kinase snf1 pour modifier la teneur en huile des organismes oléagineux Download PDF

Info

Publication number
WO2010025374A3
WO2010025374A3 PCT/US2009/055376 US2009055376W WO2010025374A3 WO 2010025374 A3 WO2010025374 A3 WO 2010025374A3 US 2009055376 W US2009055376 W US 2009055376W WO 2010025374 A3 WO2010025374 A3 WO 2010025374A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein kinase
snf1 protein
subunit
fatty acids
genes encoding
Prior art date
Application number
PCT/US2009/055376
Other languages
English (en)
Other versions
WO2010025374A2 (fr
Inventor
Seung-Pyo Hong
John E. Seip
Qun Zhu
Original Assignee
E. I. Du Pont De Nemours And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E. I. Du Pont De Nemours And Company filed Critical E. I. Du Pont De Nemours And Company
Priority to CN200980143092.6A priority Critical patent/CN102216464B/zh
Priority to JP2011525234A priority patent/JP5656836B2/ja
Priority to DK09810662.8T priority patent/DK2324120T3/en
Priority to BRPI0913138A priority patent/BRPI0913138A2/pt
Priority to EP09810662.8A priority patent/EP2324120B1/fr
Priority to AU2009285590A priority patent/AU2009285590B2/en
Priority to CA2734264A priority patent/CA2734264A1/fr
Publication of WO2010025374A2 publication Critical patent/WO2010025374A2/fr
Publication of WO2010025374A3 publication Critical patent/WO2010025374A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne des procédés permettant d’augmenter la teneur totale en lipides dans une cellule eucaryote, la teneur totale en acides gras polyinsaturés [PUFA], et/ou le rapport entre les acides gras  désaturés et les acides gras saturés par réduction de l’activité de la protéine kinase hétérotrimérique SNF1. De préférence, les gènes chromosomiques codant pour la sous-unité Snf1 χ, la sous-unité Gal83 β ou la sous-unité Snf4 γ de la protéine kinase SNF1, les gènes régulateurs en amont codant pour Sak1, Hxk2, Glk1 ou Reg1, ou les gènes en aval codant pour Rme1, Cbr1 ou Snf3 sont manipulés dans une souche de la levure oléagineuse Yarrowia lipolytica produisant des PUFA, aboutissant à une augmentation de la teneur totale en lipides, par comparaison avec la souche parente comprenant la protéine kinase hétérotrimérique SNF1 dont l’activité n’a pas été réduite.
PCT/US2009/055376 2008-08-29 2009-08-28 Manipulation de l’activité de la protéine kinase snf1 pour modifier la teneur en huile des organismes oléagineux WO2010025374A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CN200980143092.6A CN102216464B (zh) 2008-08-29 2009-08-28 操纵snf1蛋白激酶活性用于改变含油生物中的油含量
JP2011525234A JP5656836B2 (ja) 2008-08-29 2009-08-28 油性生物中の含油量の変更のためのｓｎｆ１タンパク質キナーゼ活性の操作
DK09810662.8T DK2324120T3 (en) 2008-08-29 2009-08-28 Manipulating SNF1 protein kinase OF REVISION OF OIL CONTENT IN OLEAGINOUS ORGANISMS
BRPI0913138A BRPI0913138A2 (pt) 2008-08-29 2009-08-28 célula hospedeira eucariótica oleaginosa transgênica, célula de levedura eucariótica oleaginosa transgênica, óleo ou lipídeos, método para aumentar o teor de lipídeo total de uma célula hospedeira eucariótica oleaginosa e método para aumentar o teor total de ácidos graxos poliinsaturados no óleo microbiano
EP09810662.8A EP2324120B1 (fr) 2008-08-29 2009-08-28 Manipulation de l activité de la protéine kinase snf1 pour modifier la teneur en huile des organismes oléagineux
AU2009285590A AU2009285590B2 (en) 2008-08-29 2009-08-28 Manipulation of SNF1 protein kinase activity for altered oil content in oleaginous organisms
CA2734264A CA2734264A1 (fr) 2008-08-29 2009-08-28 Manipulation de l'activite de la proteine kinase snf1 pour modifier la teneur en huile des organismes oleagineux

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9300708P 2008-08-29 2008-08-29
US61/093,007 2008-08-29

Publications (2)

Publication Number Publication Date
WO2010025374A2 WO2010025374A2 (fr) 2010-03-04
WO2010025374A3 true WO2010025374A3 (fr) 2010-05-27

Family

ID=41722312

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/055376 WO2010025374A2 (fr) 2008-08-29 2009-08-28 Manipulation de l’activité de la protéine kinase snf1 pour modifier la teneur en huile des organismes oléagineux

Country Status (9)

Country Link
US (1) US8435758B2 (fr)
EP (1) EP2324120B1 (fr)
JP (1) JP5656836B2 (fr)
CN (1) CN102216464B (fr)
AU (1) AU2009285590B2 (fr)
BR (1) BRPI0913138A2 (fr)
CA (1) CA2734264A1 (fr)
DK (1) DK2324120T3 (fr)
WO (1) WO2010025374A2 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8906650B2 (en) 2011-04-01 2014-12-09 E I Du Pont De Nemours And Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
US8735094B2 (en) 2011-04-05 2014-05-27 E I Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome P450 promoter regions for gene expression in yeast
EP2702148A1 (fr) 2011-04-07 2014-03-05 E.I. Du Pont De Nemours And Company Regions promotrices de 2,4-dienoyl-coa reductase peroxisomale de yarrowia pour l'expression genique dans la levure
EP2768954B1 (fr) * 2011-10-19 2018-12-05 Massachusetts Institute of Technology Microbes obtenus par génie génétique et procédés pour la production d'huiles microbiennes
CN102586289A (zh) * 2011-12-02 2012-07-18 华中农业大学 粘红酵母乙酰辅酶a羧化酶基因及其克隆、功能验证方法和应用
WO2015184277A1 (fr) * 2014-05-29 2015-12-03 Novogy, Inc. Augmentation de la production de lipides et optimisation de la composition lipidique
EP4174186A1 (fr) 2014-05-29 2023-05-03 Ginkgo Bioworks, Inc. Augmentation de la production de lipides dans des levures oléagineuses
US20170145449A1 (en) * 2014-06-11 2017-05-25 Institut National De La Recherche Agronomique Improved lipid accumulation in Yarrowia lipolytica strains by overexpression of hexokinase and new strains thereof
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
CN108624600B (zh) * 2018-05-22 2021-06-18 昆明理工大学 锌指转录因子基因RkMsn4的用途
CA3149454A1 (fr) * 2019-08-30 2021-03-04 Lygos, Inc. Cellules hotes recombinees pour la production de malonate
KR102335426B1 (ko) * 2020-01-07 2021-12-06 삼성전기주식회사 코일 부품
CN111321175B (zh) * 2020-03-09 2021-08-10 山东省农业科学院生物技术研究中心 一种调控集胞藻中不饱和脂肪酸合成的方法及其应用
CN112662688B (zh) * 2021-01-25 2022-04-22 山东省果树研究所 核桃SnRK1蛋白激酶编码基因JrSnRK1在油脂合成与积累中的应用
CN114317466B (zh) * 2022-01-04 2023-10-27 中山大学附属第三医院 一种突变acc1蛋白及其用途
CN116790647B (zh) * 2023-02-06 2024-04-16 森瑞斯生物科技(深圳)有限公司 一种低背景、高信号强度的2-吡咯烷酮生物传感器及应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040199940A1 (en) * 1999-07-12 2004-10-07 Balasulojini Karunanandaa Nucleic acid molecules and other molecules associated with sterol synthesis and metabolism
US20060263864A1 (en) * 1999-10-20 2006-11-23 Robert Busby Methods for improving secondary metabolite production of fungi
US20070067869A1 (en) * 2005-03-16 2007-03-22 Metabolix, Inc. Chemically inducible expression of biosynthetic pathways

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6949356B1 (en) * 1999-10-20 2005-09-27 Microbia, Inc. Methods for improving secondary metabolite production in fungi
US7238482B2 (en) * 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
CA2542564A1 (fr) * 2003-11-12 2005-05-26 E.I. Du Pont De Nemours And Company Delta-15 desaturases appropriees pour modifier les niveaux d'acides gras polyinsatures dans des plantes a graines oleagineuses et de la levure oleagineuse
US7504259B2 (en) * 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
US7202356B2 (en) 2003-11-14 2007-04-10 E. I. Du Pont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
AU2005263730B2 (en) * 2004-07-16 2011-04-28 Cropdesign N.V. Plants having improved growth characteristics and method for making the same
US7465564B2 (en) 2004-08-10 2008-12-16 E.I. Du Pont De Nemours And Company Production of γ-linolenic acid in oleaginous yeast
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US7273746B2 (en) 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7943365B2 (en) 2007-05-03 2011-05-17 E.I. Du Pont De Nemours And Company Δ-5 desaturases and their use in making polyunsaturated fatty acids
US20090117253A1 (en) 2007-10-03 2009-05-07 E. I. Du Pont De Nemours And Company Peroxisome biogenesis factor protein (pex) disruptions for altering polyunsaturated fatty acids and total lipid content in oleaginous eukaryotic organisms

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040199940A1 (en) * 1999-07-12 2004-10-07 Balasulojini Karunanandaa Nucleic acid molecules and other molecules associated with sterol synthesis and metabolism
US20060263864A1 (en) * 1999-10-20 2006-11-23 Robert Busby Methods for improving secondary metabolite production of fungi
US20070067869A1 (en) * 2005-03-16 2007-03-22 Metabolix, Inc. Chemically inducible expression of biosynthetic pathways

Also Published As

Publication number Publication date
EP2324120A2 (fr) 2011-05-25
DK2324120T3 (en) 2016-05-02
CN102216464A (zh) 2011-10-12
BRPI0913138A2 (pt) 2016-05-31
CA2734264A1 (fr) 2010-03-04
EP2324120B1 (fr) 2016-01-27
US8435758B2 (en) 2013-05-07
AU2009285590B2 (en) 2015-02-05
JP2012501185A (ja) 2012-01-19
EP2324120A4 (fr) 2013-08-14
US20100062502A1 (en) 2010-03-11
AU2009285590A1 (en) 2010-03-04
JP5656836B2 (ja) 2015-01-21
WO2010025374A2 (fr) 2010-03-04
CN102216464B (zh) 2014-11-26

Similar Documents

Publication Publication Date Title
WO2010025374A3 (fr) Manipulation de l’activité de la protéine kinase snf1 pour modifier la teneur en huile des organismes oléagineux
Economou et al. Single cell oil production from rice hulls hydrolysate
Hui et al. Direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of Aspergillus oryzae A-4 in solid-state fermentation
Huang et al. Improving lipid production from bagasse hydrolysate with Trichosporon fermentans by response surface methodology
WO2006052914A3 (fr) Acyltransferases de diacylglycerol pour la modification d'acides gras poly-insatures et de la teneur en huile dans des organismes oleagineux
dos Santos Oliveira et al. Changes in lipid, fatty acids and phospholipids composition of whole rice bran after solid-state fungal fermentation
WO2006052870A3 (fr) Souches produisant de l'acide eicosapentanoique de yarrowia lipolytica
Santos et al. Low linolenic soybeans for biodiesel: characteristics, performance and advantages
Wen et al. Optimization of nitrogen sources for heterotrophic production of eicosapentaenoic acid by the diatom Nitzschia laevis
WO2006069936A3 (fr) Procede de production d'acides gras a longues chaines multi-insatures dans des organismes transgeniques
TW200617173A (en) Process for production of microbial fat/oil containing discretional amount of diacylglycerol and said fat/oil
WO2011022229A3 (fr) Procédé de production durable améliorée de produits biologiques d'algue, comprenant l'utilisation d'une co-culture à stress atténué de diazotrophe symbiotique
WO2005003322A3 (fr) Acyltransférases destinées à modifier les acides gras polyinsaturés et la teneur en huile des levures oléagineuses
WO2010004141A3 (fr) Nouvelles souches de levure mutantes capables d'accumuler une grande quantité de lipides
WO2004076617A3 (fr) Procede de production d'acides gras polyinsatures
Pedneault et al. Fatty acid profiles of polar and non-polar lipids of Pleurotus ostreatus and P. cornucopiae var.‘citrino-pileatus’ grown at different temperatures
WO2008148845A3 (fr) Procédé de préparation d'un produit à base de pâte
Cho et al. Enhanced biomass and lipid production by supplement of myo-inositol with oceanic microalga Dunaliella salina
EP1748068A4 (fr) Procédé d'élevage d'une souche produisant des lipides et utilisation de celui-ci
EP1685239A4 (fr) delta-15 DESATURASES APPROPRIEES POUR MODIFIER LES NIVEAUX D'ACIDES GRAS POLYINSATURES DANS DES PLANTES A GRAINES OLEAGINEUSES ET DE LA LEVURE OLEAGINEUSE
WO2010104575A3 (fr) Émulsions de nutrition parentérale huile dans eau à base de poisson enrichies en oméga-3
Capusoni et al. Characterization of lipid accumulation and lipidome analysis in the oleaginous yeasts Rhodosporidium azoricum and Trichosporon oleaginosus
MX2010009652A (es) Glucoamilasa y fitasa de buttiauxella durante la sacarificacion.
AU7406201A (en) Preparation of a blend of triglycerides
MY145175A (en) Method of producing a fatty acid alkyl ester, and fuel

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200980143092.6

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09810662

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2009285590

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2734264

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2009285590

Country of ref document: AU

Date of ref document: 20090828

Kind code of ref document: A

ENP Entry into the national phase

Ref document number: 2011525234

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 2011000412

Country of ref document: CL

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2009810662

Country of ref document: EP

ENP Entry into the national phase

Ref document number: PI0913138

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20110225