WO2009154375A3 - 특정 렉틴 친화 크로마토그래피를 이용하여 혈액 유래 당단백질을 동정하는 방법 - Google Patents

특정 렉틴 친화 크로마토그래피를 이용하여 혈액 유래 당단백질을 동정하는 방법 Download PDF

Info

Publication number
WO2009154375A3
WO2009154375A3 PCT/KR2009/003166 KR2009003166W WO2009154375A3 WO 2009154375 A3 WO2009154375 A3 WO 2009154375A3 KR 2009003166 W KR2009003166 W KR 2009003166W WO 2009154375 A3 WO2009154375 A3 WO 2009154375A3
Authority
WO
WIPO (PCT)
Prior art keywords
affinity chromatography
lectin affinity
glycoprotein
specific lectin
derived glycoprotein
Prior art date
Application number
PCT/KR2009/003166
Other languages
English (en)
French (fr)
Other versions
WO2009154375A2 (ko
Inventor
고정헌
김용삼
유향숙
유종신
오세정
김철호
Original Assignee
한국생명공학연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국생명공학연구원 filed Critical 한국생명공학연구원
Publication of WO2009154375A2 publication Critical patent/WO2009154375A2/ko
Publication of WO2009154375A3 publication Critical patent/WO2009154375A3/ko

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57469—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving tumor associated glycolinkage, i.e. TAG
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 진단 대상자의 혈액에서 종양 발생 및 전이과정에서 당질변화를 보이는 당단백질을 검출해내는 방법에 관한 것으로서, 좀더 상세하게는 당단백질의 N-연결형 당쇄 중 종양의 발생과 전이에 관여한다고 알려진 특정 당쇄를 이용하여 질량분석 방법으로 당단백질을 찾아내는 방법 및 암 진단키트에 관한 것이다.
PCT/KR2009/003166 2008-06-19 2009-06-12 특정 렉틴 친화 크로마토그래피를 이용하여 혈액 유래 당단백질을 동정하는 방법 WO2009154375A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020080057652A KR20090131745A (ko) 2008-06-19 2008-06-19 특정 렉틴 친화 크로마토그래피를 이용하여 혈액 유래당단백질을 동정하는 방법
KR10-2008-0057652 2008-06-19

Publications (2)

Publication Number Publication Date
WO2009154375A2 WO2009154375A2 (ko) 2009-12-23
WO2009154375A3 true WO2009154375A3 (ko) 2010-03-18

Family

ID=41434536

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/003166 WO2009154375A2 (ko) 2008-06-19 2009-06-12 특정 렉틴 친화 크로마토그래피를 이용하여 혈액 유래 당단백질을 동정하는 방법

Country Status (2)

Country Link
KR (1) KR20090131745A (ko)
WO (1) WO2009154375A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101311412B1 (ko) * 2011-05-04 2013-09-25 한국기초과학지원연구원 당 동정을 위한 새로운 생물정보처리 분석 방법
US10697983B2 (en) 2015-09-08 2020-06-30 Merck Patent Gmbh Methods of evaluating quality of media suitable for removing anti-A or anti-B antibodies
US10697982B2 (en) 2015-09-08 2020-06-30 Merck Patent Gmbh Methods of evaluating quality of a chromatography media which binds anti-A or anti-B antibodies

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100475642B1 (ko) * 2001-12-29 2005-03-10 한국생명공학연구원 암 발생 및 전이에 관여하는 단백질의 당쇄 변화를측정하여 암을 진단하는 방법 및 이를 이용한 진단킷트
KR100771139B1 (ko) * 2005-11-22 2007-10-29 한국생명공학연구원 단백질 티로신 포스파타아제 카파를 분석하는 방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100475642B1 (ko) * 2001-12-29 2005-03-10 한국생명공학연구원 암 발생 및 전이에 관여하는 단백질의 당쇄 변화를측정하여 암을 진단하는 방법 및 이를 이용한 진단킷트
KR100771139B1 (ko) * 2005-11-22 2007-10-29 한국생명공학연구원 단백질 티로신 포스파타아제 카파를 분석하는 방법

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
SHINJI IHARA ET AL.: "Prometastatic effect of N-acetylglucosaminyltransferase V is due to modification and stabilization of active matriptase by adding beta1-6 GlcNAc branching", J. BIOL. CHEM., vol. 277, no. 19, 2002, pages 16960 - 16967 *
YINGHUA QIU ET AL.: "Plasma glycoprotein profiling for colorectal cancer biomarker identification by lectin glycoarray and lectin blot", J. PROTEOME RES., vol. 7, no. 4, April 2008 (2008-04-01), pages 1693 - 1703 *
YONG-SAM KIM ET AL.: "Functional proteomics study reveals that N- acetylglucosaminyltransferase V reinforces the invasive/metastatic potential of colon cancer through aberrant glycosylation on tissue inhibitor of metalloproteinase-1", MOL. CELL. PROTEOMICS, vol. 7, no. 1, January 2008 (2008-01-01), pages 1 - 14 *
YONG-SAM KIM ET AL.: "Identification of target proteins of N- acetylglucosaminyltransferase V in human colon cancer and implications of protein tyrosine phosphatase kappa in enhanced cancer cell migration", PROTEOMICS, vol. 6, no. 4, 2006, pages 1187 - 1191 *

Also Published As

Publication number Publication date
KR20090131745A (ko) 2009-12-30
WO2009154375A2 (ko) 2009-12-23

Similar Documents

Publication Publication Date Title
EP4249605A3 (en) Methods for analyte detection
WO2011062803A3 (en) Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms
WO2013059293A9 (en) Single, direct detection of hemoglobin a1c percentage using enzyme triggered redox altering chemical elimination (e-trace) immunoassay
WO2007002264A3 (en) Biomarker for ovarian cancer: ctap3-related proteins
WO2010135574A3 (en) Systems and methods for determining the percentage of glycated hemoglobin
NZ602056A (en) Methods and compositions for diagnosis and prognosis of renal injury and renal failure
BR112013031284A2 (pt) composições, métodos, e kits para quantificar analitos alvo em uma amostra
WO2009076139A3 (en) Thyroglobulin quantitation by mass spectrometry
WO2007100506A3 (en) Mass tags for quantitative analyses
WO2007018843A3 (en) Methods and compositions for diagnosis and treatment of influenza
WO2009103843A3 (en) Method and device for detection of an analyte
WO2012045012A3 (en) Sandwich assays in droplets
WO2010132453A3 (en) Methods and compositions for analyte detection
WO2008030305A3 (en) Methods and kits for detecting cerebrospinal fluid in a sample
WO2013066369A3 (en) Methods for detecting graft-versus-host disease
WO2010090471A3 (ko) 제1형 당뇨병의 신규한 진단 마커
WO2009129173A3 (en) Methods, antibodies and kits for detecting cerebrospinal fluid in a sample
EP1984726A4 (en) METHODS, MIXTURES, KITS AND COMPOSITIONS BELONGING TO A DETERMINATION OF SUBSTANCES TO BE ANALYZED
EP2583100A4 (en) ROTORS FOR IMMUNOASSAYS
EA200802169A1 (ru) Способы анализа гидроксикарбоната лантана
WO2010107816A3 (en) Nucleic acid binding dyes and uses therefor
WO2010092164A3 (en) Bacterial dna as markers of cardiovascular and/or metabolic disease
EP2295962A4 (en) METHOD FOR PRODUCING A SUGAR CHAIN SAMPLE, SUGAR CHAIN SAMPLE AND METHOD FOR ANALYZING THE SUGAR CHAIN
WO2010075360A3 (en) Methods for detection of sepsis
HK1165001A1 (zh) 利用免疫法測定生物樣品中的雌馬酚的方法、用於該測定的試劑盒、以及判定受試者的雌馬酚產生能力的方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09766800

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 09766800

Country of ref document: EP

Kind code of ref document: A2