WO2009149419A3 - Variant alpha-amylases from bacillus subtilis and methods of use, thereof - Google Patents

Variant alpha-amylases from bacillus subtilis and methods of use, thereof Download PDF

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Publication number
WO2009149419A3
WO2009149419A3 PCT/US2009/046506 US2009046506W WO2009149419A3 WO 2009149419 A3 WO2009149419 A3 WO 2009149419A3 US 2009046506 W US2009046506 W US 2009046506W WO 2009149419 A3 WO2009149419 A3 WO 2009149419A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
amylases
bacillus subtilis
variant alpha
amye
Prior art date
Application number
PCT/US2009/046506
Other languages
French (fr)
Other versions
WO2009149419A2 (en
Inventor
Regina Chin
William A. Cuevas
David A. Estell
Sang-Kyu Lee
Michael J. Pepsin
Scott D. Power
Sandra W. Ramer
Carol A. Requadt
Andrew Shaw
Amr R. Toppozada
Louise Wallace
Original Assignee
Danisco Us Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc. filed Critical Danisco Us Inc.
Priority to EP20090759577 priority Critical patent/EP2297313B1/en
Priority to ES09759577.1T priority patent/ES2538406T3/en
Priority to DK09759577.1T priority patent/DK2297313T3/en
Priority to JP2011512723A priority patent/JP2011522546A/en
Priority to CN200980129863.6A priority patent/CN102131924B/en
Priority to MX2010013114A priority patent/MX2010013114A/en
Priority to BRPI0915606-2A priority patent/BRPI0915606A2/en
Priority to MX2014005185A priority patent/MX364987B/en
Priority to PL09759577T priority patent/PL2297313T3/en
Priority to CA 2726803 priority patent/CA2726803A1/en
Publication of WO2009149419A2 publication Critical patent/WO2009149419A2/en
Publication of WO2009149419A3 publication Critical patent/WO2009149419A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N11/00—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

Alpha amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
PCT/US2009/046506 2008-06-06 2009-06-05 Variant alpha-amylases from bacillus subtilis and methods of use, thereof WO2009149419A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
EP20090759577 EP2297313B1 (en) 2008-06-06 2009-06-05 Variant alpha-amylases from bacillus subtilis and methods of use, thereof
ES09759577.1T ES2538406T3 (en) 2008-06-06 2009-06-05 Alpha-amylases variants of Bacillus subtilis and their methods of use
DK09759577.1T DK2297313T3 (en) 2008-06-06 2009-06-05 ALPHA AMYLASE VARIETIES OF BACILLUS SUBTILIS AND METHODS OF USING IT
JP2011512723A JP2011522546A (en) 2008-06-06 2009-06-05 Mutant alpha amylase from Bacillus subtilis and methods of use thereof
CN200980129863.6A CN102131924B (en) 2008-06-06 2009-06-05 Variant alpha amylase and its application method from bacillus subtilis
MX2010013114A MX2010013114A (en) 2008-06-06 2009-06-05 Variant alpha-amylases from bacillus subtilis and methods of use, thereof.
BRPI0915606-2A BRPI0915606A2 (en) 2008-06-06 2009-06-05 ALPHA-AMYLASES VARIANT FROM BACILLUS SUBTILIS AND METHODS OF USE OF THE SAME
MX2014005185A MX364987B (en) 2008-06-06 2009-06-05 Variant alpha-amylases from bacillus subtilis and methods of use, thereof.
PL09759577T PL2297313T3 (en) 2008-06-06 2009-06-05 Variant alpha-amylases from bacillus subtilis and methods of use, thereof
CA 2726803 CA2726803A1 (en) 2008-06-06 2009-06-05 Variant alpha-amylases from bacillus subtilis and methods of use, thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US5951308P 2008-06-06 2008-06-06
US5961808P 2008-06-06 2008-06-06
US61/059,618 2008-06-06
US61/059,513 2008-06-06

Publications (2)

Publication Number Publication Date
WO2009149419A2 WO2009149419A2 (en) 2009-12-10
WO2009149419A3 true WO2009149419A3 (en) 2010-04-22

Family

ID=41217791

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2009/046506 WO2009149419A2 (en) 2008-06-06 2009-06-05 Variant alpha-amylases from bacillus subtilis and methods of use, thereof
PCT/US2009/046480 WO2009149395A2 (en) 2008-06-06 2009-06-05 Variant alpha-amylases from bacillus subtilis and methods of use, thereof

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2009/046480 WO2009149395A2 (en) 2008-06-06 2009-06-05 Variant alpha-amylases from bacillus subtilis and methods of use, thereof

Country Status (12)

Country Link
US (4) US8323945B2 (en)
EP (2) EP2297312B1 (en)
JP (3) JP2011525350A (en)
CN (3) CN102131924B (en)
BR (2) BRPI0913367A2 (en)
CA (2) CA2726274C (en)
DK (2) DK2297312T3 (en)
ES (1) ES2538406T3 (en)
HU (1) HUE025251T2 (en)
MX (4) MX364987B (en)
PL (1) PL2297313T3 (en)
WO (2) WO2009149419A2 (en)

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