WO2009147693A2 - Procédé intégré de récupération d'une fraction polyphénol et de digestion anaérobie de déchets de moulins à olives - Google Patents

Procédé intégré de récupération d'une fraction polyphénol et de digestion anaérobie de déchets de moulins à olives Download PDF

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WO2009147693A2
WO2009147693A2 PCT/IT2009/000246 IT2009000246W WO2009147693A2 WO 2009147693 A2 WO2009147693 A2 WO 2009147693A2 IT 2009000246 W IT2009000246 W IT 2009000246W WO 2009147693 A2 WO2009147693 A2 WO 2009147693A2
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Prior art keywords
column
production
biomass
anaerobic
biogas
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PCT/IT2009/000246
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English (en)
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WO2009147693A3 (fr
Inventor
Annalisa Romani
Domenico Pangia
Luisa Marchionni
Alessandro Marchionni
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R.A.C.E. S.R.L.
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Application filed by R.A.C.E. S.R.L. filed Critical R.A.C.E. S.R.L.
Priority to EP20090758032 priority Critical patent/EP2297051A2/fr
Publication of WO2009147693A2 publication Critical patent/WO2009147693A2/fr
Publication of WO2009147693A3 publication Critical patent/WO2009147693A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00—Multistage treatment of water, waste water or sewage
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L3/00—Gaseous fuels; Natural gas; Synthetic natural gas obtained by processes not covered by subclass C10G, C10K; Liquefied petroleum gas
    • C10L3/06—Natural gas; Synthetic natural gas obtained by processes not covered by C10G, C10K3/02 or C10K3/04
    • C10L3/08—Production of synthetic natural gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/023—Methane
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/42—Treatment of water, waste water, or sewage by ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
    • C02F2103/322—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters from vegetable oil production, e.g. olive oil production
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/28—Anaerobic digestion processes
    • C02F3/286—Anaerobic digestion processes including two or more steps
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/30—Aerobic and anaerobic processes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/10—Process efficiency
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/30—Wastewater or sewage treatment systems using renewable energies

Definitions

  • the present invention concerns an integrated process for recovery of a polyphenol fraction and anaerobic digestion of olive mill wastes. More specifically, the invention concerns a treatment for the integral recovery of components and chemical compounds present in the olive mill waste waters and in the olive husks by means of membrane technologies and resins, followed by treatments of the dephenolised olive mill wastes on aerobic and anaerobic columns, as a preliminary step for the treatment thereof for biogas and high quality fertilizers having a good bioavailability in the soil.
  • the invention provides a process having the following steps: pre-dilution, centrifuga- tion; selective fractionation for the recovery of polyphenol compounds through a membrane process and adsorption on resins; pretreatment in aerobic and anaerobic attached-biomass columns and tray separation column; anaerobic biodigestion for biogas and fertilisers production; cogeneration for the production of a thermal and electrical power vector; production of water reusable in the process, having hydrochemical characteristics suitable to civil use.
  • the OMW contain mineral salts - mainly potassium and phosphate salts - and organic substances such as fats, proteins, sugars, pectins, gums and polyphenols. They have a high microbial load and consist, on average, of 83.4% water, 1.8% mineral salts and 14.8% organic compounds.
  • the organic compounds are actually responsible for the polluting potential of OMW: as a matter of fact, these are substances subject to oxidation, which may alter the environmental conditions of a possible receiving body. They can cause acidification, saturation, stagnation, impermeabilisation, inhibition and intoxication; therefore, the olive mill wastewaters, if directly introduced in the water streams, would in very short times induce anoxia phenomena due to a depletion of oxygen dissolved in water, thus damaging not only the water fauna, but also the aerobic microorganisms, that need oxygen to perform their self-depurating action.
  • any direct disposal of such wastes in the sewage system is not allowed, while only some limited amounts thereof can be employed for fertilizing irrigation (50 m 3 /ha for OMW coming from traditional discontinuous pressing oil mills and 80 m 3 /ha for OMW coming from the continuous cycle.)
  • the quality and quantity of the various OMW components is different depending on the type of apparatus employed for oil extraction.
  • the microbial transformation processes are inhibited by the presence of polyphenols; in particular, the acetogenic and methanogenic bacteria are sensitive to concentrations >0.25 g/l of caffeic acid and >0.12 g/l of p-coumaric acid.
  • Polyphenol compounds such as tyrosol and hydroxytyrosol, oleu- ropein, aglycon and the relevant hydrolysis and/or rearrangement products, phenolic acids, apigenin and luteolin (Romani et al., 2002), being transferred only in minor amounts in the oil during the oil production process (150-400 mg/l), are water-soluble, and thus they remain on large amounts in the olive mill wastes.
  • the foregoing suggests a possible way for an interesting eco- nomical recovery, deriving from valorisation and marketing of such molecules, which may be isolated from the concerned waste material.
  • the foregoing allows regarding the OMW as a "secondary starting materia! 1 for innovative productions rather than a waste product.
  • the plant may treat the following elements: ⁇ olive mill wastewaters;
  • Such substances are transformed and efficientlysed into the following products: olive oil (food quality); biodiesel (fuel); olive stones (combustible); pellets (combustible, fertilizer, animal feed); biogas (electrical power, heath, cold, and steam); irrigation water; compost.
  • the main production lines are the following ones:
  • ⁇ wastewater treatment fluid phase: physical/biological process and methanogen fermentation of the organic substances in the waste water with biogas production
  • ⁇ biogas valorisation gaseous phase: the biogas resulting from the treatment of the waste waters is transformed into thermal energy and covers the internal heath requirements
  • Figure 1 of the attached drawings shows a three-dimensional schematic representation of a platform according to the cited prior art. If, on one hand, the bio-refinery described above combines the treatment of the OMW with the recovery of matter and power, on the other hand it presents some inefficiencies in terms of productive continuity and energetic yield.
  • an object of the present invention is to provide an integrated platform, efficient and multifunctional, which allows to carry out in a preliminary phase the extraction and recovery of bioactive com- ponents from the olive mill wastes and, at the same time, to eliminate in an effective manner the polluting load of the residual materials, with production of energy in the form of biogas and high-quality fertilizers.
  • an integrated plant complex which comprises in an innovative way the following phases: pre-dilution, centrifugation, selective fractionation for the recovery of polyphenol compounds by means of a membrane process and adsorption on resins; pretreatment with aerobic and anaerobic attached-biomass columns and tray separation column; anaerobic biodigestion for biogas and fertilizer production; cogeneration for the production of a thermal and electrical power vector; pro- duction of water reusable in the process, having hydro-chemical characteristics suitable to civil use.
  • Such platform comprises a phase of anaerobic biodigestion of the OMW for the production of biogas, while including however a waste water pretreatment step, which allows the standardisation of the incoming biomass and of the inoculum necessary to start the fermentation.
  • the pretreatment process allows, in addition, to obtain an optimised energetic yield, the productive continuity and high-quality fertilizers, thus resulting in a technological innovation with respect to already existing platforms.
  • the production of polyphenol extracts from the OMW is based on a refining process taking advantage from the use of membrane separation technologies and resins.
  • the extraction technology allows to obtain the following products: ultrapure water, to be recycled in the productive process o to be used for the market of functional beverages; a raw fraction deriving from the microfiltration retentate, valorisable for the biogas production together with preliminary phase by-products; more different types of extracts, differing both in the title and in the presence of molecules of different activity and biological effectiveness, and therefore endowed with different possibilities of exploitation.
  • Hydroxytyrosol is a powerful antioxidant and cardio-protective agent (Covas M.I., et al., Nutr. Rev. 2006; 64:S20; Visioli et al., Biochem. Biophys. Res. Commun., 1998; 247:60), and is the compound having the highest functional activity in extra-virgin olive oil. It delays the LDL oxidation (Visioli F., GaIIi C, Life Sci.
  • the literature provides several studies concerning the biological activi- ties of molecules having phenilpropanoid structure, in connection with their antioxidant, anti-inflammatory, antiviral and anti-mycotic activity. Most of such studies are mainly focused to evaluate the effects of verbascoside or acteo- side, that is one of presently most studied phenilpropanoids. Verbascoside has also evidenced an in vitro immunomodulatory activity, in particular it in- creases the chemotactic activity of neutrophils; an opposed effect has been observed on increasing the dose, mainly due to a suppression of the killer activity of the neutrophil lymphocytes (Akbay P. et al., Phytother. Res. 2002; 16:593).
  • the mixture of olive industries wastes with whey, animal excrement and with about 10% of solid organic municipal waste refers to a standard composition of the OMW and olive husks (substrate), obtained separately or (as in the case the two-phases process) in a mixture.
  • Such compositions may vary depending on:
  • composition variability does not change the treatment technology and the particular features of the technological solutions; it only influences the mass and energy balance of the system:
  • Figure 1 represents an example of multifunctional platform for the production of biogas from waste materials of Olea europea L. according to the integrated process of the cited prior art:
  • Figure 2 shows a block diagram of the central section of the inte- grated process of the OMW treatment according to the invention
  • Figure 3 shows a simplified process scheme with the first section of the integrated plant according to the invention, comprising the central part of Figure 2 and a preliminary section for the separation of polyphenol compounds
  • Figure 4 shows a simplified process scheme with the second section of the integrated plant according to the invention, used for cogeneration and fertilizer production;
  • Figures 5 and 6 show the calibration curves for the analytic control techniques used in the process. DETAILED DESCRIPTION OF THE INVENTION Waste treatment before the anaerobic digestion
  • the pH of incoming olive mill wastewaters is adjusted to values in the range from 3 to 4.5, in order to inactivate the polyphenol oxidase enzyme, which would tend to oxidize the useful polyphenol compounds.
  • The, the OMW or the waste products from the two-phase olive oil extraction undergo a preliminary treatment as shown in Figure 3: pre-dilution, pre-oxidation and centri- fugation, selective fractionation for the recovery of polyphenol compounds by means of a membrane process followed by and adsorption on resins; pre- treatment in aerobic and anaerobic attached-biomass columns and in a tray separation column.
  • the pre-dilution and pre-oxidation step is intended to oxidize part of the excess polyphenols in order to prevent them from causing process blockages in the subsequent fermentation and, by means of the addition of acidifying agents or proteolytic enzymes, is aimed at potentiating the release of polyphenol compounds from the solid particles, besides catalyzing the hydrolysis of secoiridoids and of some complex molecules;
  • the centrifugation step is applied both to OMW from three-phase mill and in the case that OMW from two-phase mill are treated (in this case this step is preceded by a pre-dilution of the moist olive husks with process water, as previously described for the pre-dilution and pre-oxidation step); tangential microfiltration (MF) of the surnatant, followed by a tan- gential ultrafiltration (UF) treatment carried out on the microfiltration permeate; the retentate coming from the MF partly undergoes again the pre- dilution and partly, mixed with the UF reten
  • the previous treatment phases i.e.: pre-dilution and pre-oxidation, centrifugation, microfiltration and ultrafiltration, treatment on polymeric resins and with water, analysis before entering the pretreatment columns for the control of total polyphenols and ortho-diphenols, are subsequently repeated on the dilution and washing waters of the husks after first centrifugation of the waste and possible mixture with the MF retentate.
  • dilution and washing waters for the solid obtained from first centrifugation the waters obtained from percolation from resins ( Figure 3) are used, or those from reverse osmosis concentration, as described in the further phases of the process ( Figure 4).
  • the solid residue i.e., Fp, primary sludge, in Figures 2 and 3
  • DG1 first anaerobic digestor
  • a concentration gradient of suspended solids decreasing from the bottom to the top is created.
  • An elementary section of the column is represented by a zone comprised between two trays: the clarified fluid is transferred towards the top (i.e. at the following tray) by gravity.
  • the single trays are slightly at a slope and present an aperture in the higher part: the various apertures are aligned on the same axis.
  • the gap between one tray and the other represent the settling zone, with a hole for the discharge for the sludge discharge.
  • the said holes are in communication with the main reactor DG3.
  • the geome- trical features of the trays are as follows: a) The holes have a variable diameter, not lower than 1 cm and not higher than 10 cm; b) The ratio of the fluid rates through the apertures for the up-flowing fluid (clarified fluid) and those through the holes for the sludge is not lower than 5.
  • Such column is a multilayer packed column, with the following packing
  • the function of the inert packing is to supply an anchoring to the biomass and thus create some zones (biofilm) wherein a very high concentration of biomass occurs; this results in minimising the inhibition factors.
  • the activated carbon in addition, performs a selective adsorption action, mainly in respect of enzyme complexes, while the bricks have a more complex function:
  • the fluid dynamic effect is made evident by the separation of sus- pended solids and by their deposition on the brick walls, and it is due to acceleration and deceleration phenomenon that the fluid particles undergo while passing through the channel system of the brick.
  • the bricks packing has a complex function, that may be synthe- sised as follows: - it causes the formation of a superficial layer which serves to accumulate and hold biomass and bacteria; the rates of bacterial catabolism and anabolism are increased in the biomass layer between the bricks and the fluid, since an exchange of matter is established: the brick supplies inorganic microelements and adsorbs macromolecular components.
  • the fluid exiting the column is returned in part to the methanogenic reactor, and in part is fed the following aerobic column (CAA).
  • CAA aerobic column
  • CAA Fluidized bed aerobic attached biomass column
  • the clarified fluid exiting the previous column goes to a fluid- ized bed combined aeration column fed with the dephenolised OMW and husks (AVSd).
  • the fluidized bed is made out by sand and activated carbon.
  • the fluidized bed has the function of a biomass fixator, while the function of activated carbon is not fully clear. Very likely, the activated carbon: - exerts an effective action of biomass accumulation; and
  • the microorganisms pool to be periodically inoculated in the reactors, and precisely in the CAA and CAN columns and in the DG2 digestor may consist of the following microorganisms: 1. methanogen bacteria: isolated from the CAN column medium; adapted collection microorganisms: Methanosaeta concihi Klebisella oxytoca Clostridium propionicum Leuconostoc mesenteroides Clostridium butyricum Desulfovibriovulgaria Methanobacterium formicicum Metahanosarcina barkeri
  • hydrolytic and hydrogen-productive bacteria isolated from the DG2 anaerobic digestor medium
  • aerobic microorganisms isolated from the CAA column medium adapted collection microorganisms Candida rugosa Candida tropicalis Phanerochete chrysosporium Pleurotus ostreatus Laeptiporus sulphureus Pleurotus sajor-caju Funalia trogii Paecilomyces variotii Lentinus tigrinus Anaerobic digestion phase and biogas production
  • the process starts from dephenolised OMW and husks (AVSd), and refers to a standard composition referred to average values as defined in the scientific literature, and to substrates actually employed in the tests.
  • composition (AVd) OMW resulting from the extraction of antioxidants, dyes
  • the anaerobic digestion is a process carried out over liquid or semi- solid fractions (substrate), on which anaerobic microorganisms grow. Due to such microbial activity the organic substance is degraded and methane and other minor gases (carbon dioxide, hydrogen, hydrogen sulphide, etc.) are produced.
  • the proposed process according to the invention is carried out, preferably, in two digestion stages: a preliminary anaerobic stage (DG1 ), a second stage (DG2) carried out in anaerobic conditions and with an inoculums of hydrolytic and hydrogen producing microorganisms, previously listed under item 2 (acidophilic stage) and a third stage (DG3) anaerobic as well, wherein the biogas production mostly takes place (methanogenic stage).
  • DG1 preliminary anaerobic stage
  • DG2 second stage
  • DG3 anaerobic as well, wherein the biogas production mostly takes place
  • the second stage is characterised by biochemical reactions leading to the reduction of the molecular masses through enzymatic hydrolysis of complex substances (cellulosic and polymeric substances, and high molecular weight organic molecules) and acidophilic reactions with formation of simple substances; this stage is characterised by the formation of low molecular weight organic acid (acetic, succinic, propionic acid, etc.
  • the third stage is defined "methanogenic stage", in that it is based on the development of a microbial flora which uses the low molecular weight organic acid formed in the previous stage to form biogas.
  • the relationship between the second and the third reaction stages is of a fundamental importance for the working stability of the process; actually, in the third stage the methanogenic biochemical reactions are characterised by the transformation of short chain acids in methane: said reaction is thermo- dynamically unfavoured, as in "standard” conditions it has a positive free energy variation ( ⁇ G).
  • ⁇ G free energy variation
  • the proposed tech- nology results from a thorough search on laboratory scale and on a pilot plant, aiming at the following objects:
  • the abatement of the organic load represents the substrate quantity used for the production of biogas. Such abatement is measured usually through two parameters:
  • the produced biogas is a gaseous mixture that is expressed, on aver- age, by the following composition : methane % v/v 65 % w/w 42 .50 hydrogen % v/v 2 % w/w 0 .17 carbon dioxide % v/v 30 % w/w 53 .91 ammonia %v/v 1 % w/w 0 .70 hydrogen sulphide %v/v 1 % w/w 1 .40 oxygen % v/v 1 % w/w 1 .32 density kg/m 3 1.092 density relative to air 0.843 average molecular weight t PM m 24.47 lower calorific value kcal/nmc 5500
  • a SSV concentration of the OMW (unitary %)
  • the biogas with 65% methane produced will fluctuate by a +/- 50% with respect to the average values
  • the results do not change in terms of biogas productivity; only the amount of aqueous residue editing the digestor is modified g.
  • Chemical energy-electric power conversion The produced biogas with 65% methane feeds an internal combustion engine for the production of electric power. The following data are assumed:
  • the thermal Energy is used for:
  • the fluids involved in the combustion and produced by it may be characterised in that they are involved in the design of downstream heat exchangers. h. Liquid fraction
  • the fermented substrate from the anaerobic digestion for the part thereof which is not re-circulated to the process, is filtered on a belt filter press o on a filter press and a solid fraction and a liquid fraction are separated.
  • the liquid fraction contains elements essential to the soil fertility, and in particular it contains carbon and nitrogen in soluble form, mostly of biological origin: this enormously increases the fertilizing value of the product.
  • the liquid fraction contains the basic elements for fertility: - soluble phosphorus
  • the liquid fraction is concentrated by means of a reverse osmosis process carried out on tubular membranes. Then, in order to reach the con- centration provided for by the legislative Decree of April 26 2006 N. 217 of the
  • Organomineral NPK fertilizer Title 12% % (N+P 2 O 5 + K 2 O) + 3,0% organic carbon Possibly, additions of substances based on N, P and K may be foreseen in order to reach the title.
  • Organomineral NPK fertilizer Title 15% (N+P 2 O 5 + K 2 O) + 7,5% organic carbon
  • N, P and K may be foreseen in order to reach the title.
  • Formulation with different concentration of fertilizing unit may be foreseen: this is obtained by varying the concentration process of the liquid or solid material.
  • the calibration curve, in gallic acid, is shown in the enclosed Figure 5.
  • 10 mg in 10 ml in hydro-alcoholic solution are weighted, dilutions are carried out so as to obtain ranges from 10 to 150 ⁇ g/ml and the curve is constructed considering that in the procedure dilution is made 10 times.
  • the spectrophotometric reading of the sample must fall in the linearity interval of the calibration line, so that the starting sample will have to be suita- bly diluted.
  • reactive B sodium molybdate and sodium nitrite (10 g of Na 2 MoO 4 * 2H2O + 10g of NaNO 2 dissolved in 100 ml of H 2 O)
  • reactive C NaOH 1 N (4 g dissolved in 100 ml of distilled H 2 O)
  • the mixture is stirred and centrifuged for 30 minutes, and then it is read at 500 m.
  • the enclosed Figure 6 shows an example of curve with 3,4- dihydroxybenzoic acid.
  • growth factor unknown growth factor
  • the invention solves the serious problems of the treatment of the olive mill waste waters, at level of single olive mill or consortia, thus reducing the problems of transportation of huge volumes of wastes, with a reduction of transportation costs and apparent environmental benefits, namely avoiding the spreading on the soils of a phytotoxic industrial effluent, contaminating for the water tables.
  • Simple substances such as simple perphosphate, ammonium sulphate, potassium sulphate, ammonium nitrate.
  • the system for the production of natural biofertilizers, liquid and solid opens a new productive scenarios, also extended to other wastes of the agro- industry.
  • the amounts involved may be relevant so as to substitute a remark- able percentage of the present use of synthesis fertilizers, thus enhancing the reduction of the use of chemistry in agriculture.

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Abstract

L'invention concerne un procédé utilisant des technologies à membrane pour récupérer de manière intégrée des éléments et des composants chimiques d'eaux usées de moulins à olives et de grignons d'olives, des résines et des traitements du produit de matière déphénolisée à l'aide de colonnes de biomasse anaérobie et aérobie associées et d'une colonne de séparation à plateau aérobie, comme phase préliminaire en vue de l'exploitation desdits déchets pour la production de biogaz et d'engrais de qualité supérieure et à disponibilité supérieure dans le sol. Le traitement décrit est caractérisé en ce qu'il comporte les étapes suivantes: prédilution, centrifugation; fractionnement sélectif pour la récupération des composés de polyphénol au moyen d'un procédé à membrane et d'une adsorption sur résines; prétraitement dans des colonnes de biomasse anaérobie et aérobie associées et une colonne de séparation à plateau; biodigestion anaérobie en vue de la production de biogaz et d'engrais; cogénération en vue de la production d'un vecteur d'énergie thermique et électrique; production d'eau réutilisable dans le procédé et qui présente des caractéristiques hydrochimiques convenant pour un usage civil.
PCT/IT2009/000246 2008-06-05 2009-06-05 Procédé intégré de récupération d'une fraction polyphénol et de digestion anaérobie de déchets de moulins à olives WO2009147693A2 (fr)

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Application Number Priority Date Filing Date Title
EP20090758032 EP2297051A2 (fr) 2008-06-05 2009-06-05 Procédé intégré de récupération d'une fraction polyphénol et de digestion anaérobie de déchets de moulins à olives

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Application Number Priority Date Filing Date Title
IT000004A ITFG20080004A1 (it) 2008-06-05 2008-06-05 Trattamento per digestione anaerobica delle acque di vegetazione da frantoio oleario e delle sanse da molitura delle olive dopo estrazione dalle stesse della frazione polifenolica, del siero di latte con produzione di biogas e concimi organici comple
ITFG2008A000004 2008-06-05

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WO2009147693A2 true WO2009147693A2 (fr) 2009-12-10
WO2009147693A3 WO2009147693A3 (fr) 2010-06-10

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WO2011095805A1 (fr) * 2010-02-03 2011-08-11 Cpi Innovation Services Limited Procédés et installation de traitement pour le traitement de flux de déchets organiques aqueux
EP2390235A1 (fr) * 2010-05-31 2011-11-30 André Holzer Procédé et installation pour le traitement de matériau de déchet organique liquide
ITBR20100003A1 (it) * 2010-06-08 2011-12-09 Planungsburo Rossow "procedimento integrato per produrre biogas e per ottenere acqua libera da sostanze inquinanti da acque di vegetazione in uscita dai frantoi oleari"
DE102012107396A1 (de) * 2012-08-13 2014-02-13 4Biogas Gmbh & Co. Kg Verfahren zum Anfahren einer Biogasanlage
WO2014193466A1 (fr) * 2013-05-29 2014-12-04 Oberon Fmr, Inc. Traitement des eaux usées pour la production de biomasse microbienne
CN113849776A (zh) * 2021-11-19 2021-12-28 中译语通科技（青岛）有限公司 一种用于厨余废弃物处理厌氧工艺的优化方法
EP4011854A1 (fr) 2020-12-11 2022-06-15 Consejo Superior de Investigaciones Científicas (CSIC) Utilisation de la fraction liquide du digestat des déchets solides d'un moulin à huile dans les traitements de fertirrigation
GR1010388B (el) * 2018-11-07 2023-01-26 Ανεστης Αποστολου Βλυσιδης Μεθοδος εξαγωγης φαινολικων ενωσεων απο τα υπολειμματα της παραγωγικης διαδικασιας εξαγωγης ελαιολαδου
GR1010529B (el) * 2022-05-19 2023-08-29 Αποστολος Γεωργιου Βλυσιδης Μεθοδος βιωσιμης και ολοκληρωμενης επεξεργασιας και αξιοποιησης των υπολειμματων που προκυπτουν απο την εξαγωγη του ελαιολαδου, με παραγωγη προϊοντων υψηλης προστιθεμενης αξιας

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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011095805A1 (fr) * 2010-02-03 2011-08-11 Cpi Innovation Services Limited Procédés et installation de traitement pour le traitement de flux de déchets organiques aqueux
EP2390235A1 (fr) * 2010-05-31 2011-11-30 André Holzer Procédé et installation pour le traitement de matériau de déchet organique liquide
ITBR20100003A1 (it) * 2010-06-08 2011-12-09 Planungsburo Rossow "procedimento integrato per produrre biogas e per ottenere acqua libera da sostanze inquinanti da acque di vegetazione in uscita dai frantoi oleari"
DE102012107396A1 (de) * 2012-08-13 2014-02-13 4Biogas Gmbh & Co. Kg Verfahren zum Anfahren einer Biogasanlage
EP2698424A1 (fr) * 2012-08-13 2014-02-19 4Biogas GmbH & Co. KG Procédé de démarrage d'une installation de biogaz
US20160108480A1 (en) * 2013-05-29 2016-04-21 Oberon Fmr, Inc. Wastewater treatment for the production of microbial biomass
WO2014193466A1 (fr) * 2013-05-29 2014-12-04 Oberon Fmr, Inc. Traitement des eaux usées pour la production de biomasse microbienne
US10214786B2 (en) * 2013-05-29 2019-02-26 Procell Investments Limited Wastewater treatment for the production of microbial biomass
GR1010388B (el) * 2018-11-07 2023-01-26 Ανεστης Αποστολου Βλυσιδης Μεθοδος εξαγωγης φαινολικων ενωσεων απο τα υπολειμματα της παραγωγικης διαδικασιας εξαγωγης ελαιολαδου
EP4011854A1 (fr) 2020-12-11 2022-06-15 Consejo Superior de Investigaciones Científicas (CSIC) Utilisation de la fraction liquide du digestat des déchets solides d'un moulin à huile dans les traitements de fertirrigation
CN113849776A (zh) * 2021-11-19 2021-12-28 中译语通科技（青岛）有限公司 一种用于厨余废弃物处理厌氧工艺的优化方法
CN113849776B (zh) * 2021-11-19 2022-03-11 中译语通科技（青岛）有限公司 一种用于厨余废弃物处理厌氧工艺的优化方法
GR1010529B (el) * 2022-05-19 2023-08-29 Αποστολος Γεωργιου Βλυσιδης Μεθοδος βιωσιμης και ολοκληρωμενης επεξεργασιας και αξιοποιησης των υπολειμματων που προκυπτουν απο την εξαγωγη του ελαιολαδου, με παραγωγη προϊοντων υψηλης προστιθεμενης αξιας

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