WO2009127443A2 - Facteurs de transcription mis en jeu dans un stress dû au sel dans des plantes - Google Patents

Facteurs de transcription mis en jeu dans un stress dû au sel dans des plantes Download PDF

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Publication number
WO2009127443A2
WO2009127443A2 PCT/EP2009/002979 EP2009002979W WO2009127443A2 WO 2009127443 A2 WO2009127443 A2 WO 2009127443A2 EP 2009002979 W EP2009002979 W EP 2009002979W WO 2009127443 A2 WO2009127443 A2 WO 2009127443A2
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Prior art keywords
seq
polynucleotide
plant
loc
sequence
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PCT/EP2009/002979
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WO2009127443A3 (fr
Inventor
Bernd Müller-Röber
Slobodan Ruzicic
Camila Caldana
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Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V
Universität Potsdam
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Publication of WO2009127443A2 publication Critical patent/WO2009127443A2/fr
Publication of WO2009127443A3 publication Critical patent/WO2009127443A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

Definitions

  • Salinity is a major environmental stress, affecting plant growth and development and it represents an increasing threat to plant agriculture. The impact of these factor increases continuously and leads on the global level to a decline of average yields by more than 50%. High salt depositions alter the basic structure of the soil reducing its porosity and consequently its water potential and aeration, making it difficult for plants to acquire water and nutrients, and it also increases the sensitivity to diverse biotic stresses.
  • better agricultural practices i.e., water and soil management
  • the introduction of salt-tolerant varieties in the affected areas have been approached.
  • the improvement of irrigation management practices in salt-affected areas is usually uneconomical and difficult to implement on large scale.
  • desalinization programmes cause environmental injuries.
  • Soil salinity is thus one of the more important variables that determines where a plant may thrive.
  • sizable land areas are uncultivable due to naturally high soil salinity.
  • salination of soils that are used for agricultural production is a significant and increasing problem in regions that rely heavily on agriculture. The latter is compounded by over-utilization, over-fertilization and water shortage, typically caused by climatic change and the demands of increasing population.
  • Salt tolerance is of particular importance early in a plant's lifecycle, since evaporation from the soil surface causes upward water movement, and salt accumulates in the upper soil layer where the seeds are placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt level in the whole soil profile.
  • QTLs quantitative trait loci
  • TF transcription factor
  • Transcription factors are key controlling elements of biological pathways, therefore altering their expression levels can change entire biological pathways in an organism. For example, manipulation of the levels of selected transcription factors may result in increased expression of economically useful proteins or biomolecules in plants or improvement in other agriculturally relevant characteristics. Conversely, blocked or reduced expression of a transcription factor may reduce biosynthesis of unwanted compounds or remove an undesirable trait. Therefore, manipulating transcription factor levels in a plant offers tremendous potential in agricultural biotechnology for modifying a plant's traits, including traits that improve a plant's survival and yield during salt stress other abiotic stresses.
  • the present invention relates to an isolated polynucleotide capable of giving a plant tolerance to abiotic stress, particularly salt stress and/or osmotic stress, which consists of a polynucleotide 5 sequence selected from the group comprising SEQ ID NO. 1 to SEQ ID NO. 253.
  • the present invention also relates to an expression vector comprising the said polynucleotide and/or a promoter capable of giving a plant tolerance to abiotic stress, preferred osmotic stress and/or salt stress, and to a host cell transformed or transfected by the said expression vector.
  • the present invention further relates to a use of said polynucleotide or promoter sequence in improvement of plant tolerance to abiotic 0 stress, preferred osmotic stress and/or salt stress.
  • SEQ ID NO 1 LOC_Os01g08710.2: ATGTTCCCATCACCAGGGAGGGCGGTGATGGCGCTAGGCCACCACGGCGCCGCCCGCCAACCGGCGA
  • SEQ ID NO 18 LOC_Os01g52540.1: ATGGC TGAAGTAATGAGCATATTGGATGTTGACAAAGTGACAGTTGAATTATTCTGTGCAATGCTTGTTTTCTATAAATGGAATGTGGATGCGGTGGCA GAAGACTTTGACATATGTAGGGGCAAACCGCAAATTCAGAACCTGTTTCTGAAGCACAAACTTCAr ⁇ TCAATTTGATATTGTAAAGAGGAAACT
  • SEQ ID NO 66 LOC_Os03g19630 1 CGACCTGTTCCTCGGCCCCGRGCTGGACCTGCTGCTGGACTACCTGGCCGACACCGACCCGAACCGTCAGGGCACGCCGCCGGCCAGGAA
  • LOC_Os03g49880 1 ATGCTTCCTTTCTTCGATTCCCC SEQ ID NO 74: LOC_Os03g50310 1 ATGG
  • SEQ ID NO 102 LOC_Os04g47860 5 ATGGCATC ATGGCATCGAATTCATCGGCTGCAGCTGCGG
  • SEQ ID NO 110 LOC_Os04g51400.4: ATGGATGA
  • SEQ ID NO 111 LOC_Os04g51400.5: ATGGATGATC
  • SEQ ID NO 115 LOC_Os05g03900.1 : ATGGAAAA AATCAACAAAGI I I I I GCTGATGAGAATGAAGCTTTTGACTTCTACAATGGTTATGCTTATATGGTTGGTTTCTCTACATGCAAAGCTAGCAATT
  • LOC_Os06g41060.1 ATGGAGCTTCTCAAG
  • SEQ ID NO 154 LOC_Os07g047005 ATGAACA GTCAGATGTTGAGAAAGAGAAACTTGCTAAAGGAATAAAGCAGCAATACCAAGAGGCATTGATTTTGAACTCGATCAATAAAGGAAGTTCCACC
  • SEQ ID NO 158 LOC_Os07g 2 ATGCAGCCGGTACGGCCATCTGACGGAGTTCGA
  • SEQ ID NO 160 LOC_Os07g37210 1 A
  • LOC_Os08g45110 1 ATGATTCTGATAC
  • LOC_Os09g28210 1 ATGGACTTCGACTTGTTCA AAGCGCCACGTGCTCAAGGTGTGGATGCACAACAACAAGCACACCCTGGGCAAGAAGCTGCCATGA SEQ ID NO 198: LOC_Os09g29930.2:
  • SEQ ID NO 206 LOC_Os10g02910.1: A CAATCACTAGAGCTTGTGTTAAAGTATATGAAGATGAGAAAGAAAAGCTTAAGAAATTTTTTAAGGACAATTGTGTAAGAGTTTGCCTCACAACT
  • LOC_Os11g02530.1 ATGAAGA ATGGTGGAGCCTGTGAAATCGGAGCTTGGCAGC ID NO 225: LOC_Os11gO841O 1 ATGCCGAAGCCGA CTAAGAATCCTTTTGCCGTGCAAATAATGATGGAGTCATATGTCTATGTTGGATTTTTCATGAATATCCCATGTGAATTTGTCCGTGAGTGTCTT GTGCACATCTACAGAGTTGTCCCAGAAATTACTCCGCACAAACTCCGTTCTGACCCGAAGTAA SEQ ID NO 227: LOC_Os11g11220 1 ATGG
  • LOC_Os12g38950 1 ATGGCTTCTGATGTTCCTCAAGATGACGTGCAATGCCATTTTTGTGGC ATACCAACATCTGCTACAAGAGCGACGCATTCAGAAATGGCTTCTGATGTTCCTCAAGGTTACGTGCAATGTCATTTTTGTGACACCTACTTAA
  • ID NO 248 LOC_Os12g40070 1 ATGGGTGATCAAAAA
  • TTCTACTGGGCTAA SEQ ID NO 250: LOC_Os12g424Q02 ATGATGAGCTTCAACAAGAGCCAAGAAGGATTTGGGCAGGTTGCTGCTGTGGC
  • LOC_Os12g42400 3 ATGATGAGCTTCAA CCATTTGCAGAATATAATGGCTGTTTTGAGCTGGGCCTTGGTCAATCTGTGGTTCCCTCTAATTATCCTTATGCTGACCAGCACTATGGCCTAC
  • an isolated polynucleotide selected from the group comprising: (a) polynucleotide consisting of a sequence selected from the group comprising SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 14 to SEQ ID NO. 17, SEQ ID NO. 20 to SEQ ID NO. 23, SEQ ID NO. 25, SEQ ID NO. 27, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 44 to SEQ ID NO. 46, SEQ ID NO. 48, SEQ ID NO. 52, SEQ ID NO. 56 to SEQ ID NO. 58, SEQ ID NO. 61 to SEQ ID NO. 67, SEQ ID NO. 69, SEQ ID NO. 71,
  • a polynucleotide consisting of a sequence having at least 70%, preferred 80%, more preferred 90, especially preferred 98% sequence identity to a sequence selected from the group comprising SEQ DD NO. 1 , SEQ DD NO. 2, SEQ DD NO. 14 ro SEQ DD NO.
  • Polynucleotides of the present invention that are variants of the polynucleotides provided herein will generally demonstrate significant identity with the polynucleotides provided herein.
  • polynucleotide homologs having at least about 60% sequence identity, at least about 70% sequence identity, at least about 80% sequence identity, at least about 85% sequence identity, and 5 more preferably at least about 90%, 95% or even greater, such as 98% or 99% sequence identity with polynucleotide sequences described herein.
  • sequence identity refers to a measure of relatedness between two or more nucleic acids, and is given as a percentage with reference to the total comparison length. The identity calculation takes into account those nucleotide residues that are identical and in the same relative positions in their respective larger sequences.
  • a partially complementary sequence is one that at least partially inhibits (or competes with) a completely complementary sequence from hybridizing to a target nucleic acid.
  • the inhibition 5 of hybridization of the completely complementary sequence to the target sequence may be examined using a hybridization assay (Southern or Northern blot, solution hybridization and the like) under conditions of low stringency.
  • a substantially homologous sequence or probe will compete for and inhibit the binding (in other words, the hybridization) of a sequence which is completely homologous to a target under conditions of low stringency. This is not to say that conditions of low stringency are 0 such that non-specific binding is permitted; low stringency condiii ⁇ ns require that the binding of two sequences to one another be a specific (in other words, selective) interaction.
  • the absence of nonspecific binding may be tested by the use of a second target which lacks even a partial degree of complementarity (for example, less than about 30% identity); in the absence of non-specific binding the probe will not hybridize to the second non-complementary target.
  • the polynucleotides of the present invention may be present in the form of DNA, such as cDNA or genomic DNA, or as RNA, for example mRNA.
  • the polynucleotides of the present invention may be single or double stranded and may represent the coding, or sense strand of a gene, or the non-coding, antisense, strand. i0
  • isolated refers to a polynucleotide or polypeptide molecule separated from substantially all other molecules normally associated with it in its native state. More preferably, a substantially purified molecule is the predominant species present in a preparation. A substantially purified
  • 15 molecule may be greater than 60% free, preferably 75% free, more preferably 90% free, and most preferably 95% free from the other molecules (exclusive of solvent) present in the natural mixture.
  • isolated is also used herein in reference to polynucleotide molecules that are separated from nucleic acids which normally flank the polynucleotide in nature. Thus, polynucleotides fused to regulatory or coding sequences with which they are not normally associated, for example as the result of recombinant techniques, are considered isolated herein. Such molecules are considered isolated even when present, for example in the chromosome of a host cell, or in a nucleic acid solution.
  • isolated and purified as used herein are not intended to encompass molecules present in their native state.
  • abiotic stress is the negative impact of non-living factors on the living organisms.
  • the non-living variable influences the environment beyond its normal range of variation to adversely affect the population performance or individual physiology of the organism.
  • Abiotic stress factors, or stressors are naturally occurring, often intangible, factors such as intense sunlight or wind that may cause harm to the plants and animals in the area affected. Abiotic stress comes in many forms.
  • the stressors include: high winds, extreme temperatures, heat, cold, strong light, water deficit, drought, flood, and other natural disasters, such as tornados and wildfires, poor edaphic conditions like rock content and pH, high radiation, compaction, contamination, non-optimal nutrient or salt levels, non-optimal light levels and other, highly specific conditions like rapid rehydration during seed germination.
  • tolerant or “tolerance” refers to the ability of a plant to overcome, completely or to some degree, the detrimental effect of an environmental stress or other limiting factor.
  • the transgenic plants are preferred tolerant to conditions including, but not limited to osmotic stress, particularly salt stress.
  • “Expression” means the production of a protein or nucleotide sequence in the cell itself or in a cell-free system. It includes transcription into an RNA product, post-transcriptional modification and/or translation to a protein product or polypeptide from a DNA encoding that product, as well as possible post-translational modifications.
  • an isolated polynucleotide selected from the group comprising:
  • nucleotide sequence encoding a polypeptide, wherein said nucleotide sequence is selected from the group consisting of SEQ ID NO. 1 , SEQ ID NO. 2, SEQ ID NO. 14 to SEQ DD NO.
  • SEQ ID NO. 111 SEQ ID NO. 1 17, SEQ ID NO. 120, SEQ ID NO. 122 to SEQ ID NO. 124, SEQ ID NO. 131 to SEQ ID NO. 133, SEQ ID NO. 135, SEQ ID NO. 136, SEQ ID NO. 139 to SEQ ID NO. 141, SEQ ID NO. 144, SEQ ID NO. 146 to SEQ ID NO. 148, SEQ ED NO. 152, SEQ ID NO. 157 to SEQ ID NO. 159, SEQ ID NO. 162, SEQ ID NO. 165 to SEQ ID NO167, SEQ ID NO. 170, SEQ ID NO. 171, SEQ ID NO. 180, SEQ ID NO. 183 to SEQ ID NO.
  • nucleotide sequence encoding a polypeptide, wherein said polypeptide is selected from the group consisting of SEQ ID NO. 254, SEQ ID NO. 255, SEQ ID NO. 267 to SEQ ID NO. 270, SEQ ID NO. 273 to SEQ ID NO. 276, SEQ ID NO. 278, SEQ ID NO. 280,SEQ ID NO. 293, SEQ ID NO. 294, SEQ ID NO. 297 to SEQ ID NO. 299, SEQ ID NO. 301, SEQ ID NO.
  • (c) a variant of any of the nucleotide sequences of (a) or (b) that has at least 70%, preferred 80%, more preferred 90%, especially 98% sequence identity to a sequence of (a) or (b)
  • the invention relates to said isolated polynucleotide, wherein the polynucleotide is selected from the group comprising SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 14 to SEQ ID NO. 17, SEQ ED NO. 20 to SEQ ED NO. 23, SEQ ED NO. 25, SEQ ED NO. 27, SEQ ED NO. 40, SEQ ED NO. 41, SEQ ED NO. 44 to SEQ ED NO. 46, SEQ ID NO. 48, SEQ ED NO. 52, SEQ ED NO. 56 to SEQ ED NO. 58, SEQ ID NO. 61 to SEQ ED NO. 67, SEQ ED NO. 69, SEQ ID NO.
  • these sequences can be used to stabilize the photosynthetic activity during salt stress and/or osmotic stress, which results in a stabilized photosynthetic yield.
  • These sequences were highly responsive and therefore very suitable for transfection.
  • the invention relates to an isolated polynucleotide selected from the group comprising SEQ ID NO. 144, SEQ ID NO. 146 to SEQ ID NO. 148, SEQ ID NO. 152, SEQ ID NO. 157 to SEQ ID NO. 159, SEQ ID NO. 162, SEQ ID NO. 165 to SEQ ID NOl 67, SEQ ID NO. 170, SEQ ID NO. 171, SEQ ID NO. 180, SEQ ID NO. 183 to SEQ ID NO. 186, SEQ ID NO. 190 to SEQ ID NO. 194, SEQ ID NO. 197 to SEQ ID NO. 200, SEQ ID NO. 205, SEQ ID NO. 208, SEQ ID NO.
  • sequences can be used to alter a plants tolerance to salt and osmotic stress particularly well. Plants comprising these sequences or modifications thereof showed a better growth during salt and/or osmotic stress compared to control plants.
  • the polynucleotides) ⁇ f the present invention find particular use in generation ot transgenic plants to provide for increased or decreased expression of the polypeptides encoded by the polynucleotides provided herein.
  • plants, particularly crop plants, having improved properties are obtained.
  • Crop plants of interest in the present invention include, but are not limited to soy, cotton, canola, maize, wheat, sunflower, sorghum, alfalfa, barley, millet, rice, tobacco, fruit and vegetable crops, and turf grass.
  • nucleotide construct comprising said polynucleotide, wherein said polynucleotide is operably linked-to a promoter that drives expression in a plant cell.
  • the polynucleotides of the present invention find particular use in generation of transgenic plants to provide for increased or decreased expression of the polypeptides encoded by the polynucleotides provided herein.
  • plants, particularly crop plants, having improved properties are obtained.
  • Crop plants of interest in the present invention include, but are not limited to soy, cotton, canola, maize, wheat, sunflower, sorghum, alfalfa, barley, millet, rice, tobacco, fruit and vegetable crops, and turf grass.
  • the invention relates to a nucleotide construct comprising a one of the mentioned polynucleotide, wherein said polynucleotide is operably linked to a promoter that drives expression in a plant cell.
  • operably linked is intended a functional linkage between a promoter and a second sequence, wherein the promoter sequence initiates and mediates transcription of the DNA sequence corresponding to the second sequence.
  • operably linked means that the nucleic acid sequences being linked are contiguous and, where necessary to join two protein coding regions, contiguous and in the same reading frame.
  • the cassette may additionally contain at least one 10 additional gene to be cotransformed into the organism. Alternatively, the additional gene(s) can be provided on multiple expression cassettes.
  • Such constructs are useful for production of transgenic plants having at least one improved property as the result of expression of a polypeptide of this invention.
  • Improved properties of interest include 15 stress tolerance, preferred abiotic stress tolerance preferred osmotic stress tolerance and/or salt stress tolerance, yield, disease resistance and growth rate.
  • a construct will generally include a plant promoter to direct transcription of the protein-encoding region or the antisense sequence of choice or gene-specific antisense region of sequence or gene- 20 specific region of sequence appropriate for the design of gene-specific artificial micro RNA.
  • a plant promoter to direct transcription of the protein-encoding region or the antisense sequence of choice or gene-specific antisense region of sequence or gene- 20 specific region of sequence appropriate for the design of gene-specific artificial micro RNA.
  • Numerous promoters which are active in plant cells, have been described in the literature. Further preferred is the nucleotide construct, wherein said promoter is a constitutive promoter., especially preferred a tissue-preferred promoter.
  • nucleotide construct wherein said promoter is an inducible promoter, preferred a stress-inducible promoter.
  • the invention relates to a polypeptide encoded by said polynucleotide and/or said nucleotide construct.
  • polypeptides consisting of a sequences selected from the group comprising SEQ ID NO. 254 to SEQ ID NO. 506.
  • SEQ ID NO. 254 LOC_Os01g08710.2: MFPSPGRAVMALGHHGAARQPPTTMAAAASSSTTSAAAAPATATTTVAFSFQHPTPTPSHHHHHHGVL
  • EGRHVHSPSRDDDDAARASAEMTFIW* SEQ ID NO. 255: LOC_Os01g08710.1: MALGHHGAARQPPTTMAAAASSSTTSAAAAPATATTTVAFSF ⁇ f ⁇ ⁇
  • EQ ID NO. 256 LOC_Os01g14010.1 MATSEAAAISNPFAPLTNHQQEHPPPPPPPAKKKRNLPGTPDPEAEVIALSPRTLMATNRFVCEICGKGFQR
  • LOC_Os01g29840 1 MGEQQQQVERQPDLPPGFRFHPTDEEIITFYLAPKWDSRGFCVAAIGEVDLNKCEPWDLPGKAKMNGEKEWYFYCQKDR SEQ ID NO. 260: LOC_Os01g32920 1 ID NO. 261: LOC_Os01g39020 1 MLKPQTPRARRAA
  • SEQ ID NO. 265 LOC_Os01g45730
  • SEQ ID NO. 274 LOC_O LOC_Os01g56070 2 MENRVGESSATAVDGGGGAKDSG GGLFPLLSFQVHGFPQAAAYGPAAGFPYGYGHSFHGWHGHGFPHQAPQGQHVDVFLKVLLVLVGVLVIASLIVF* SEQ ID NO. 277: LOC_Os01g6 RLYYRCSYREDRQCLASKLVQQENDDDPPLYRVTYTYEHTCNTTPVPTPDWAEQPPPGAAGDAYLLRFGSSAGGGGGGAHQQQTERERQQN
  • GSSYGNTMN SEQ ID NO. 295 LOC_Os01g73460 1 MGRRALPPSSSSSSSSSTTTTSPELRRKRTAAPPPPPSPRRYRSISDVMRRSLPVDAAP
  • SEQ ID NO. 303 LOC_Os02g35560.
  • GALP SEQ ID NO. 316 LOC_Os03g08470 2 MCGGAILAEFIPAPSRAAAATKRVTASHLWPAGSKNAARGKSKSKRQQRSFADVDDFEAAFEQF
  • LOC_Os03g51690 3 MCRGGLQVGAPPEVAARLTAVAQDLELRQRTALGVLGAATEPELDQFMEAYHEMLVKYREELTRPLQEA HYKWPYPSESQKVALAESTGLDLKQINNWFINQRKRHWKPSDEMQFVMMDGYHPTNAAAFYMDGHFINDGGLYRLG* SEQ ID NO. 332 LOCJDs RLRAENRELAARLHAVARHGLAARCQNARLRAEAAALARRLLALQRLARGRHMMITASPPQFSRR'SEQ ID NO. 333 LOC_Os03g59460 1 MASS
  • LOC_Os04g47860 MASNSSAAAAAAFF SMPARADEQ* SEQ ID NO 358 LOC_Os04g47860 7 MASNSSAAAAAAFFGISRDGDQHDQIKPLISHQQHQHQQQQLAASLTGVATAAPTAASS QGAPPAAPPAKKKRNLPGNPSNQPKYPFTISAMHAYISVLRDLVSIDWSLIICFLTVKASYRSHRRA* SEQ ID NO 359 LOC_Os04g48350 1 MEW
  • LOC_Os05g50350 1 MASAAGSKQQQAMMSLPSSRGGGGGGWTQRQNKQFECALAVYDKETPDRWHNIARYM GGAKSADEVRRHFDHLVEDVSRIESGRVPFPRYSSSSSSRGADDGNRSRYLKYQ * SEQ ID NO. 384 LOC_Os05g50700 1 MTITHASSLSRFHPL
  • LOC_Os09g28210 1 MDFDLFNSYPESQLDL TTPVDWQMGADEAGSNQLWDGLQDLMKLDEADTWFPPFSGAASSF* SEQ ID NO. 450 LOC_Os09g29130 1 MDFDDHDDGDEEMPPMPVSS PSGSGSGKKRFRTKFTQEQKDKMLAFAERVGWRIQKHDEAAVQQFCDEVGVKRHVLKVWMHNNKHTLGKKLP * SEQ ID NO.451 LOC_Os09g2 99302 SEQ ID NO.452 LOC_Os09g29930 3 MDSGSRS 1 MGIQGNKATTREHDFLSLYTTAAKDPSLQLH
  • LOC_Os12g42400 1 MMSFNKSQEGFGQ
  • a polypeptide consisting of a sequence selected from the group comprising SEQ ID NO. 254, SEQ ID NO. 255, SEQ ID NO. 267 to SEQ ID NO. 270, SEQ ID NO. 273 to SEQ ID NO. 276, SEQ ID NO. 278, SEQ ID NO. 280,SEQ ID NO. 293, SEQ ID NO. 294, SEQ ID NO. 297 to SEQ ID NO. 299, SEQ ID NO. 301 , SEQ ID NO. 305, SEQ ID NO. 309 to SEQ ID NO.
  • polypeptide consisting of a sequence having at least 70%, preferred 80%, more preferred 90%, especially preferred 98% sequence identity to a sequence selected from the group comprising SFQ ID NO. 254, SEQ ID NO. 255, SEQ ID NO. 267 io SEQ ID NO. 270, SEQ
  • polypeptide means an unbranched chain of amino acid residues that are covalently linked by an amide linkage between the carboxyl group of one amino acid and the amino group of another.
  • polypeptide can encompass whole proteins (i.e. a functional protein encoded by a particular gene), as well as fragments of proteins.
  • proteins Of particular interest are polypeptides of the present invention which represent whole proteins or a sufficient portion of the entire protein to impart the relevant biological activity of the protein.
  • protein also includes molecules consisting of one or more polypeptide chains. Thus, a polypeptide of the present invention may also constitute an entire gene product, but only a portion of a functional oligomeric protein having multiple polypeptide chains.
  • polypeptides involved in one or more important biological properties in plants are polypeptides involved in one or more important biological properties in plants.
  • Such polypeptides may be produced in transgenic plants to provide plants having improved phenotypic properties and/or improved response to stressful environmental conditions.
  • decreased expression of such polypeptides may be desired, such decreased expression being obtained by use of the polynucleotide sequences provided herein, for example in antisense or cosuppression methods.
  • Polypeptides of the present invention that are variants of the polypeptides provided herein will generally demonstrate significant identity with the polypeptides provided herein.
  • polypeptides having amino acid sequences provided herein reference polypeptides
  • functional homologs of such reference polypeptides wherein such functional homologs comprises at least 50 consecutive amino acids having at least 90% identity to a 50 amino acid polypeptide fragment of said reference polypeptide.
  • protein(s) when used herein refer to amino acids in a polymeric form of any length. Said terms also include known amino acid modifications such as disulphide bond formation, cysteinylation, oxidation, glutathionylation, methylation, acetylation, farnesylation, biotinylation, stearoylation, formylation, lipoic acid addition, phosphorylation, sulphation, ubiquitination, myristoylation, palmitoylation, geranylgeranylation, cyclization (e.g. pyroglutamic acid formation), oxidation, deamidation, dehydration, glycosylation (e.g. pentoses, hexosamines, N-acetylhexosamines, deoxyhexoses, hexoses, sialic acid etc.), acylation and radiolabels
  • Polypeptides useful for improved stress tolerance under a variety of stress conditions include polypeptides involved in gene regulation, such as ion antiporters, ion transporters, H + pyrophosphatases, H + ATPases, aquaporines, CNGCs, glutamate receptors, Ca 2+ - ATPases, transcription factors, serine/threonine-protein kinases, MAP kinases, MAP kinase kinases, and MAP kinase kinase kinases; polypeptides that act as receptors for signal transduction and regulation, such as receptor protein kinases; intracellular signaling proteins, such as protein phosphatases, GTP binding proteins, and phospholipid signaling proteins; polypeptides involved in arginine biosynthesis; polypeptides involved in ATP metabolism, including for example ATPase, adenylate transporters, and polypeptides involved in ATP synthesis and transport; polypeptides involved in glycine be
  • polypeptide wherein said polypeptide is a transcription factor.
  • Transcription factors play a key role in plant growth and development by controlling the expression of one or more genes in spatial, temporal and physiological specific patterns. Enhanced or reduced activity of such polypeptides in transgenic plants will provide significant changes in gene transcription patterns and provide a variety of beneficial effects in plant growth, development and response to environmental conditions.
  • Transcription factors of interest include, but are not limited to ABBVPl, C3H, HRT, SBP, Alfin-like, CAMTA, HSF, Sigma70-like, AP2-EREBP, CCAAT, LFY, SRS, ARF, CPP, LM, TAZ, ARR-B, CSD, MADS, TCP, BBR/BPC, DBP, MYB, Trihelix, BESl, E2F-DP, MYB-related, TUB, bHLH, EIL, NAC, ULT, bZIP, FHA, Orphans, VOZ, C2C2-CO-like, G2-like, PBF-2-like, WRKY, C2C2-Dof, GeBP, PLATZ, zf-HD, C2C2-GATA, GRAS, Pseudo ARR-B, ZIM, C2C2-YABBY, GRF, RWP-RK, C2H2, HB, Sl Fa-like, AR
  • any of a variety of polynucleotide sequences are capable of encoding the transcription factors and transcription factor homologue polypeptides of the invention. Due to the degeneracy of the genetic code, many diffident polynucleotides can encode identical and/or substantially similar polypeptides in addition to those sequences illustrated in the Sequence Listing. Nucleic acids having a sequence that differs from the sequences shown in the Sequence Listing, or complementary sequences, that encode functionally equivalent peptides (i.e., peptides having some degree of equivalent or similar biological activity) but differ in sequence from the sequence shown in the sequence listing due to degeneracy in the genetic code, are also within the scope of the invention.
  • amino acids have analogous physicochemical properties so that these amino acids advantageously can be replaced by each other.
  • these include the group of nonpolar (hydrophobic) amino acids (a) glycine, alanine, valine, leucine and/or isoleucine; or the hydroxy amino acids (b) serine, threonine and/or tyrosine, the amides of amino dicarboxylic acids (c) asparagine and glutamine, the amino dicarboxylic acids (d) aspartic acid and glutamic acid; the basic amino acids (e) lysine, arginine and/or ornithine as well as the group of aromatic amino acids (f) phenylalanine, tyrosine and/or tryptophan.
  • nonpolar amino acids a) glycine, alanine, valine, leucine and/or isoleucine
  • hydroxy amino acids b) serine, threonine and/or tyrosine
  • amino acids by structural similar amino acids.
  • this is the case in the group with a ⁇ - functional group (g) cysteine, methionine, serine, ⁇ - aminobutyric acid and selenocysteine as well as the turn-inducing group (h) proline, 1 -amino-2-carboxy cyclohexane, pipecolic acid and an ortho- aminobenzoic acid.
  • a-h the same group
  • peptide sequences will have a sufficient homology to be an analogous to an amino acid sequence of the peptides of the invention.
  • the amino acids can be replaced by modified amino acids or specific enantiomers.
  • the invention relates to a vector comprising said polynucleotide and/or said nucleotide construct.
  • the vector is a viral expression vector, a phage display vector, a bacterial expression vector, a yeast expression vector, a vector for expression in insects cells, a vector for in-vitro expression, a mammalian expression vector, a fungus expression vector, an algae expression vector or a plant expression vector.
  • vector is meant a DNA sequence, which can be introduced in an organism by transformation and can be stably maintained in said organism.
  • Vector maintenance is possible in e.g. cultures of Escherichia coli, Agrobacterium tumefaciens, Saccharomyces cerevisiae or Schizosaccharomyces pombe.
  • Other vectors such as phagemids and cosmid vectors can be maintained and multiplied in bacteria and/or viruses.
  • Vector sequences generally comprise a set of unique sites recognised by restriction enzymes, the multiple cloning site (MCS), wherein one or more non-vector sequence(s) can be inserted.
  • MCS multiple cloning site
  • “Expression vectors” form a subset of vectors which, by virtue of comprising the appropriate regulatory sequences enabling the creation of an expressible format for the inserted non- vector sequence(s), thus allowing expression of the protein encoded by said non-vector sequence(s).
  • Expression vectors are known in the art enabling protein- (gene-) expression in organisms including bacteria (e.g. Escherichia coli), fungi (e.g. Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris), insect cells (e.g. baculoviral expression vectors), animal cells (e.g. COS or CHO cells) and plant cells (e.g. potato virus X-based expression vectors, see e.g. Vance et al. 1998— WO9844097).
  • the current invention clearly includes any vector or expression vector comprising a non- vector DNA sequence encoding a polypeptide of the invention, homologue and/or derivative.
  • Vectors may also include a screenable marker.
  • Screenable markers may be used to monitor transformation.
  • Exemplary screenable markers include antibiotic resistant genes or genes expressing a colored or fluorescent protein such as a luciferase or green fluorescent protein (GFP), a beta- glucuronidase or uidA gene (GUS), which encodes an enzyme for which various chromogenic substrates are known or an R-locus gene, which encodes a product that regulates the production of anthocyanin pigments (red color) in plant tissues.
  • GFP green fluorescent protein
  • GUS beta- glucuronidase
  • uidA gene GUS
  • Other possible selectable and/or screenable marker genes will be apparent to those of skill in the art.
  • the invention relates to a host cell comprising said polynucleotide, said nucleotide construct, said polypeptide and/or said vector.
  • the host cell wherein the host cell is selected from the group comprising a bacterial cell, a yeast cell, a fungus cell, a mammalian cell, an insect cell, an algae cell and/or a plant cell.
  • the invention relates to a transgenic plant cell having stably incorporated into its genome at least one nucleotide construct comprising said polynucleotide, operably linked to a promoter that drives expression in said cell.
  • transgenic organism is one whose genome has been altered by the incorporation of foreign genetic material or additional copies of native genetic material, e.g. by transformation or recombination.
  • a "transgenic plant” refers to a plant that contains genetic material not found in a wild type plant of the same species, variety or cultivar.
  • the genetic material may include a transgene, an insertional mutagenesis event (such as by transposon or T-DNA insertional mutagenesis), an activation tagging sequence, a mutated sequence, a homologous recombination event or a sequence modified by chimeraplasty.
  • the foreign genetic material has been introduced into the plant by human manipulation, but any method can be used as one of skill in the art recognizes.
  • a transgenic plant may contain an expression vector or cassette.
  • the expression cassette typically comprises a polypeptide-encoding sequence operably linked (i.e., under regulatory control of) to appropriate inducible or constitutive regulatory sequences that allow for the expression of polypeptide.
  • the expression cassette can be introduced into a plant by transformation or by breeding after transformation of a parent plant.
  • a plant refers to a whole plant as well as to a plant part, such as seed, fruit, leaf, or root, plant tissue, plant cells or any other plant material, e.g., a plant explant, as well as to progeny thereof, and to in vitro systems that mimic biochemical or cellular components or processes in a cell.
  • Transgenic plant in terms of the invention does also relate to cisgenic plants.
  • Plant or "Plants” comprise all plant species which belong to the superfamily Viridiplantae.
  • the present invention is applicable to any plant, in particular monocotyledonous plants and dicotyledonous plants including a fodder or forage legume, ornamental plant, food crop, tree, or shrub selected from the list comprising Acacia spp., Acer spp., Actinidia spp., Aesculus spp., Agathis australis, Albizia amara, Alsophila tricolor, Andropogon spp., Arachis spp, Areca catechu, Astelia fragrans, Astragalus cicer, Baikiaea plurijuga, Betula spp., Brassica spp., Bruguiera gymnorrhiza, Burkea africana, Butea frondosa, Cadaba farinosa, Calliandra spp., Camellia sinensis, Carina indica, Capsicum
  • Polynucleotides or DNA constructs of the invention may be introduced into the genome of the desired plant host by a variety of conventional techniques.
  • the DNA construct may be introduced directly into the genomic DNA of the plant cell using techniques such as electroporation and microinjection of plant cell protoplasts, or the DNA constructs can be introduced directly to plant tissue using ballistic methods, such as DNA particle bombardment.
  • the DNA constructs may be combined with suitable T-DNA flanking regions and introduced into a conventional
  • Agrobacterium tumefaciens host vector The virulence functions of the Agrobacterium tumefaciens host will direct the insertion of the construct and adjacent marker into the plant cell DNA when the cell is infected by the bacteria.
  • Microinjection techniques are known in the art and well described in the scientific and patent literature.
  • the introduction of DNA constructs using polyethylene glycol precipitation is described in Paszkowski et al. Embo J. 3:2717-2722 (1984).
  • Electroporation techniques are described in Fromm et al Proc. Natl. Acad. Sci. USA 82:5824 (1985).
  • Ballistic transformation techniques are described in Klein et al. Nature 327:70-73 (1987).
  • Agrobacterium tumefaciens-mediated transformation techniques including disarming and use of binary vectors, are well described in the scientific literature. See, for example Horsch et al. Science 233:496-498 (1984), and Fraley et al. Proc. Natl. Acad. Sci. USA 80:4803 (1983).
  • Transformed plant cells which are derived by any of the above transformation techniques can be cultured to regenerate a whole plant which possesses the transformed genotype and thus the desired phenotype.
  • the expression cassette is stably incorporated in transgenic plants and confirmed to be operable, it can be introduced into other plants by sexual crossing. Any of a number of standard breeding techniques can be used, depending upon the species to be crossed.
  • transgenic plant having an altered tolerance to abiotic stress, preferred osmotic stress and/or salt stress compared to a wild-type plant, wherein the transgenic plant comprises at least one modified polynucleotide, wherein the modified polynucleotide is selected from the group comprising an overexpressed polynucleotide, a suppressed polynucleotide and/or a knocked out polynucleotide.
  • transgenic plant having an altered tolerance to abiotic stress preferred osmotic stress and/or salt stress compared to a control plant, wherein the transgenic plant comprises at least one of said polynucleotides, and the control plant does not overexpress a polypeptide encoded by the polynucleotide.
  • transgenic plant comprising at least one said polypeptides.
  • Salt stress tolerance can be assayed according to any of a number of well-know techniques. For example, plants can be grown under conditions in which more than optimum salt concentration is provided to the plant. Salt stress tolerance can be determined by any of a number of standard measures including turgor pressure, growth, yield and the like, yield of photosynthesis, stomatal conductivity, transpiration rate, osmotic potential etc.
  • transgenic plant wherein the transgene comprises a polynucleotide sequence that hybridizes under stringent conditions to the said complement polynucleotide.
  • hybridize refers to the pairing of complementary nucleic acids. Hybridization and the strength of hybridization (in other words, the strength of the association between the nucleic acids) is impacted by such factors as the degree of complementary between the nucleic acids, stringency of the conditions involved, the T n , of the formed hybrid, and the G:C ratio within the nucleic acids.
  • T n refers to the “melting temperature” of a nucleic acid.
  • the melting temperature is the temperature at which a population of double-stranded nucleic acid molecules becomes half dissociated into single strands.
  • the equation for calculating the T m of nucleic acids is well known in the art.
  • stringent conditions refers to the conditions of temperature, ionic strength, and the presence of other compounds such as organic solvents, under which nucleic acid hybridizations are conducted.
  • transgenic plant wherein the plant is selected from the group consisting of poales, preferred poaceae, more preferred ehrhartoideae and/or panicideae, especially preferred rice and/or maize.
  • transgenic plant wherein said plant is a crop plant or a monocot or a cereal, such as maize, wheat, barley, millet, rye, sorghum, oats preferred rice; or a plant cell derived from said transgenic plant.
  • a crop plant or a monocot or a cereal such as maize, wheat, barley, millet, rye, sorghum, oats preferred rice; or a plant cell derived from said transgenic plant.
  • transgenic plant wherein the abiotic stress is osmotic stress, preferred salt stress.
  • Rice is one of the most important alimentary corps.
  • the tolerance to abiotic stress, preferred osmotic stress and/or salt stress is particularly important for rice.
  • transgenic plant wherein the transgenic plant is a cultured host cell.
  • the invention also relates to a seed produced from the transgenic plant, preferred a transformed seed.
  • the invention relates to a seed produced fi ⁇ rn the transgenic plant.
  • the invention also relates to a method for producing one of said plants, said method comprising the steps of transforming a target plant with an expression vector comprising a polynucleotide, encoding a transcription factor polypeptide.
  • the transformed plant has a morphology that is substantially similar to a control plant.
  • a method for altering a plant stress response comprising stably introducing into the genome of a plant at least one nucleotide construct comprising a polynucleotide operably linked to a promoter that drives expression in a plant cell, wherein said polynucleotide is selected from the group comprising:
  • polynucleotide consisting of a sequence selected from the group comprising SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 14 to SEQ ID NO. 17, SEQ ID NO. 20 to SEQ ID NO. 23, SEQ ID NO. 25, SEQ ID NO. 27, SEQ ID NO. 40, SEQ ED NO. 41, SEQ ID NO. 44 to SEQ ID NO. 46, SEQ ID NO. 48, SEQ ID NO. 52, SEQ ID NO. 56 to SEQ ID NO. 58, SEQ ID NO. 61 to SEQ ID NO. 67, SEQ ID NO. 69, SEQ ID NO. 71, SEQ ID NO. 75, SEQ ID NO. 78, SEQ ID NO. 79, SEQ ID NO.
  • SEQ ID NO. 84 SEQ ID NO. 85, SEQ ID NO. 87, SEQ ID NO. 88, SEQ ID NO. 95, SEQ ID NO. 98 to SEQ ID NO. 111, SEQ ID NO. 117, SEQ ID NO. 120, SEQ ID NO. 122 to SEQ ID NO. 124, SEQ ID NO. 131 to SEQ DD NO. 133, SEQ DD NO. 135, SEQ DD NO. 136, SEQ DD NO. 139 to SEQ DD NO. 141, SEQ DD NO. 144, SEQ DD NO. 146 to SEQ DD NO. 148, SEQ DD NO. 152, SEQ DD NO. 157 to SEQ DD NO. 159, SEQ DD NO. 162, SEQ DD NO. 165 to SEQ DD NO167, SEQ ID NO. 170, SEQ DD NO. 171, SEQ DD NO.
  • a method for improving the yield of a plant comprising stably incorporating into the genome of said plant at least one nucleotide construct comprising a polynucleotide operably linked to a promoter that drives expression in a plant cell, wherein said polynucleotide is selected from the group comprising:
  • polynucleotide consisting of a sequence selected from the group comprising SEQ ID • NO. 1, SEQ DD NO. 2, SEQ DD NO. 14 to SEQ DD NO. 17, SEQ DD NO. 20 to SEQ DD NO. 23, SEQ ID NO. 25, SEQ DD NO. 27, SEQ DD NO. 40, SEQ DD NO. 41, SEQ DD NO. 44 to SEQ DD NO. 46, SEQ DD NO. 48, SEQ DD NO. 52, SEQ DD NO. 56 to SEQ
  • Yield refers to increased plant growth, increased crop growth, increased biomass, and/or increased plant product production, and is dependent to some extent on temperature, plant size, organ size, planting density, light, water and nutrient availability, and how the plant copes with various stresses, such as through temperature acclimation and water or nutrient use efficiency.
  • Salt-stressed plants experience a decrease of their photosynthetic efficiency (Munns, 1993). This decrease seems to be associated with the photosystem II (PSII) complex.
  • Salinity stress decreases the PSII activity and promotes the destruction of chlorophyll pigments via accumulation of ions that may inhibit the quantum yield of PSII electron transport (Sudhir and Murthy, 2004).
  • the ratio of F VV F M - i.e., F v >: variable fluorescence from light-adapted material; F M -: the maximum fluorescence signal, when all PSII centres are in the closed state
  • F VV F M - i.e., F v >: variable fluorescence from light-adapted material
  • F M - the maximum fluorescence signal, when all PSII centres are in the closed state
  • Figure 1 shows the photosynthetic rate analysed in wild-type and os02gl 3800-1 plants grown at two different salt stress concentrations, i.e., 5OmM NaCl (left panel) and 10OmM NaCl (right panel). Data are mean ⁇ SE of eight replicates plants eachs.
  • High salt concentration represents a water deficit or osmotic stress because of decreased osmotic potential in the soil or hydroponic solution.
  • solutes are accumulated as a response to falling water potential of the cell's environment.
  • the osmotic potential of the cell is lowered, which in turn attracts water into the cell helping to maintain turgor pressure water deficit (Babu et al., 1999).
  • the effects of osmotic stress caused by high salinity were measured in leaves of plants with reduced expression level of the gene Os02g46030 (ps02g46030- ⁇ ) and wild-type plants. Salt-treated plants (i.e., wild-type and transgenic plants) showed a slight decrease in the osmotic potential three days after the onset of the stress
  • Figure 3 shows the response of transpiration and (B) the stomatal conductance in os02gl 3800-1 transgenic (black bars) and Nipponbare wild-type (grey bars) seedlings upon application of salt stress (5OmM NaCl) for up to three days. Data are mean ⁇ SE of four replicates.
  • Leaves of plants with increased expression level of the gene Os06g41060 (35S::Os06g41060) and Nipponbare wild-type plants were compared employing gas exchange as physiological parameter. Transpiration rate and stomatal conductance were analysed in plants 0 and 48h after start of the salt stress (5OmM NaCl; Figure 4). At 48h, leaves of Nipponbare showed a reduction by 35% of the original transpiration rate, whereas the 35S::Os06g41060 displayed a decline of only 21%. Stomatal conductance was less significantly different between the plants, with a reduction of 30 and 38%, respectively, of the initial rates detected in the mutant and the wild-type, respectively.
  • Figure 4 shows the response of transpiration and (B) the stomatal conductance: 35S::Os06g41060 (black bars) and Nipponbare (grey bars) plants treated by salt stress (5OmM NaCl) for two days. Data are means ⁇ SE of six replicates.

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Abstract

L'invention porte sur des polynucléotides isolés, des facteurs de transcription préférés, des polypeptides codés par les polynucléotides, des vecteurs et des cellules hôtes comprenant le polynucléotide et des plantes transgéniques comprenant le polynucléotide ou le polypeptide. L'invention porte également sur un procédé pour modifier une réponse à un stress dans des plantes, de préférence un stress dû au sel et/ou un stress osmotique.
PCT/EP2009/002979 2008-04-17 2009-04-17 Facteurs de transcription mis en jeu dans un stress dû au sel dans des plantes WO2009127443A2 (fr)

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CN102199610A (zh) * 2011-03-30 2011-09-28 上海市农业生物基因中心 水稻基因OsHSF01的用途
CN103255148A (zh) * 2013-04-28 2013-08-21 中国农业科学院作物科学研究所 水稻转录因子Os01g18440基因的应用
CN106282393A (zh) * 2016-10-27 2017-01-04 中国农业科学院作物科学研究所 特异引物对及其在检测水稻耐盐性中的应用
CN110592100A (zh) * 2019-10-08 2019-12-20 海南大学 一种木薯camta基因及其抑制表达载体的构建和抗病应用
CN112501181A (zh) * 2020-12-04 2021-03-16 福建省亚热带植物研究所 水稻抗逆相关基因OsTZF7及其编码蛋白与应用
CN113512549A (zh) * 2021-03-15 2021-10-19 华南农业大学 一种利用突变OsHsfC2a基因提前水稻生育期及提高产量的方法
CN114940994A (zh) * 2022-04-29 2022-08-26 宁波大学 一类水稻转录因子OsNF-YA在水稻抗病毒中的应用
CN115747225A (zh) * 2022-08-08 2023-03-07 东北林业大学 一种山新杨PdbGRF1基因及其应用
CN116064587A (zh) * 2022-11-09 2023-05-05 广西大学 一种水稻耐盐相关的OsWRKY18基因及在调控耐盐胁迫中的应用

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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102199610A (zh) * 2011-03-30 2011-09-28 上海市农业生物基因中心 水稻基因OsHSF01的用途
CN103255148A (zh) * 2013-04-28 2013-08-21 中国农业科学院作物科学研究所 水稻转录因子Os01g18440基因的应用
CN103255148B (zh) * 2013-04-28 2014-08-13 中国农业科学院作物科学研究所 水稻转录因子Os01g18440基因的应用
CN106282393A (zh) * 2016-10-27 2017-01-04 中国农业科学院作物科学研究所 特异引物对及其在检测水稻耐盐性中的应用
CN110592100B (zh) * 2019-10-08 2022-08-02 海南大学 一种木薯camta基因及其抑制表达载体的构建和抗病应用
CN110592100A (zh) * 2019-10-08 2019-12-20 海南大学 一种木薯camta基因及其抑制表达载体的构建和抗病应用
CN112501181A (zh) * 2020-12-04 2021-03-16 福建省亚热带植物研究所 水稻抗逆相关基因OsTZF7及其编码蛋白与应用
CN113512549A (zh) * 2021-03-15 2021-10-19 华南农业大学 一种利用突变OsHsfC2a基因提前水稻生育期及提高产量的方法
CN114940994A (zh) * 2022-04-29 2022-08-26 宁波大学 一类水稻转录因子OsNF-YA在水稻抗病毒中的应用
CN114940994B (zh) * 2022-04-29 2023-07-07 宁波大学 一类水稻转录因子OsNF-YA在水稻抗病毒中的应用
CN115747225A (zh) * 2022-08-08 2023-03-07 东北林业大学 一种山新杨PdbGRF1基因及其应用
CN115747225B (zh) * 2022-08-08 2024-03-29 东北林业大学 一种山新杨PdbGRF1基因及其应用
CN116064587A (zh) * 2022-11-09 2023-05-05 广西大学 一种水稻耐盐相关的OsWRKY18基因及在调控耐盐胁迫中的应用

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