WO2009117853A1 - Procédé pour cultiver des plantes ayant une capacité d’absorption d’azote augmentée - Google Patents

Procédé pour cultiver des plantes ayant une capacité d’absorption d’azote augmentée Download PDF

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Publication number
WO2009117853A1
WO2009117853A1 PCT/CN2008/000610 CN2008000610W WO2009117853A1 WO 2009117853 A1 WO2009117853 A1 WO 2009117853A1 CN 2008000610 W CN2008000610 W CN 2008000610W WO 2009117853 A1 WO2009117853 A1 WO 2009117853A1
Authority
WO
WIPO (PCT)
Prior art keywords
sequence
promoter
fragment
dna molecule
plant
Prior art date
Application number
PCT/CN2008/000610
Other languages
English (en)
Chinese (zh)
Inventor
王志兴
刘昱辉
贾士荣
伍祥贵
包骏
Original Assignee
北京优利康生物农业技术有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 北京优利康生物农业技术有限公司 filed Critical 北京优利康生物农业技术有限公司
Priority to PCT/CN2008/000610 priority Critical patent/WO2009117853A1/fr
Publication of WO2009117853A1 publication Critical patent/WO2009117853A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un procédé pour cultiver des plantes ayant une capacité d’absorption d’azote augmentée par transformation de vecteurs d’expression recombinants contenant un promoteur spécifique de racine et le gène NAT3 en des tissus ou des cellules de plante pour obtenir des plantes transgéniques croissant normalement dans des conditions pauvres en azote. Le procédé a un large champ d’application dans l’absorption d’engrais azoté par les plantes et la sélection de variétés de plantes ayant une tolérance à la déficience en azote.
PCT/CN2008/000610 2008-03-27 2008-03-27 Procédé pour cultiver des plantes ayant une capacité d’absorption d’azote augmentée WO2009117853A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2008/000610 WO2009117853A1 (fr) 2008-03-27 2008-03-27 Procédé pour cultiver des plantes ayant une capacité d’absorption d’azote augmentée

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2008/000610 WO2009117853A1 (fr) 2008-03-27 2008-03-27 Procédé pour cultiver des plantes ayant une capacité d’absorption d’azote augmentée

Publications (1)

Publication Number Publication Date
WO2009117853A1 true WO2009117853A1 (fr) 2009-10-01

Family

ID=41112913

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2008/000610 WO2009117853A1 (fr) 2008-03-27 2008-03-27 Procédé pour cultiver des plantes ayant une capacité d’absorption d’azote augmentée

Country Status (1)

Country Link
WO (1) WO2009117853A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102577725A (zh) * 2012-01-16 2012-07-18 四川农业大学 云烟97烤烟品种的施肥方法
US8722072B2 (en) 2010-01-22 2014-05-13 Bayer Intellectual Property Gmbh Acaricidal and/or insecticidal active ingredient combinations
US9265252B2 (en) 2011-08-10 2016-02-23 Bayer Intellectual Property Gmbh Active compound combinations comprising specific tetramic acid derivatives
CN110655563A (zh) * 2019-11-01 2020-01-07 中国海洋大学 一种浒苔中硝酸盐转运蛋白体及其编码基因
CN114561400A (zh) * 2022-03-07 2022-05-31 安徽农业大学 红颜草莓硝酸盐转运蛋白基因FaNRT1.1及其应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5459252A (en) * 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
CN1434869A (zh) * 1999-12-16 2003-08-06 普拉通公司 根特异性启动子
US20050044585A1 (en) * 1996-02-14 2005-02-24 Good Allen G. Plants with enhanced levels of nitrogen utilization proteins in their root epidermis and uses thereof
CN1737148A (zh) * 2005-07-15 2006-02-22 南开大学 高亲和铵转运因子融合基因及其在转基因植物中的应用
CN1887903A (zh) * 2006-07-19 2007-01-03 北京优利康生物农业技术有限公司 硅藻的硝酸盐转运蛋白及其编码基因与应用

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5459252A (en) * 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US20050044585A1 (en) * 1996-02-14 2005-02-24 Good Allen G. Plants with enhanced levels of nitrogen utilization proteins in their root epidermis and uses thereof
CN1434869A (zh) * 1999-12-16 2003-08-06 普拉通公司 根特异性启动子
CN1737148A (zh) * 2005-07-15 2006-02-22 南开大学 高亲和铵转运因子融合基因及其在转基因植物中的应用
CN1887903A (zh) * 2006-07-19 2007-01-03 北京优利康生物农业技术有限公司 硅藻的硝酸盐转运蛋白及其编码基因与应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NITZ, I. ET AL.: "Pykl 0, a seedling and root specific gene and promoter from Arabidopsis thaliana", PLANT SCIENCE, vol. 161, no. 2, July 2001 (2001-07-01), pages 337 - 346, XP001013257, DOI: doi:10.1016/S0168-9452(01)00412-5 *
YAMAMOTO, Y.T. ET AL.: "Characterization of cis-acting sequences regulating root-specific gene expression in tobacco.", PLANT CELL, vol. 3, no. 4, April 1991 (1991-04-01), pages 371 - 382, XP002980317, DOI: doi:10.1105/tpc.3.4.371 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8722072B2 (en) 2010-01-22 2014-05-13 Bayer Intellectual Property Gmbh Acaricidal and/or insecticidal active ingredient combinations
US9265252B2 (en) 2011-08-10 2016-02-23 Bayer Intellectual Property Gmbh Active compound combinations comprising specific tetramic acid derivatives
CN102577725A (zh) * 2012-01-16 2012-07-18 四川农业大学 云烟97烤烟品种的施肥方法
CN110655563A (zh) * 2019-11-01 2020-01-07 中国海洋大学 一种浒苔中硝酸盐转运蛋白体及其编码基因
CN110655563B (zh) * 2019-11-01 2021-03-19 中国海洋大学 一种浒苔中硝酸盐转运蛋白体及其编码基因
CN114561400A (zh) * 2022-03-07 2022-05-31 安徽农业大学 红颜草莓硝酸盐转运蛋白基因FaNRT1.1及其应用

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