WO2009091168A2 - Procédé destiné à réhabiliter un sol pollué à l'aide d'un corps poreux inorganique - Google Patents

Procédé destiné à réhabiliter un sol pollué à l'aide d'un corps poreux inorganique Download PDF

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Publication number
WO2009091168A2
WO2009091168A2 PCT/KR2009/000165 KR2009000165W WO2009091168A2 WO 2009091168 A2 WO2009091168 A2 WO 2009091168A2 KR 2009000165 W KR2009000165 W KR 2009000165W WO 2009091168 A2 WO2009091168 A2 WO 2009091168A2
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WO
WIPO (PCT)
Prior art keywords
porous body
inorganic porous
contaminated soil
microorganisms
soil
Prior art date
Application number
PCT/KR2009/000165
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English (en)
Korean (ko)
Other versions
WO2009091168A3 (fr
Inventor
Bong Han Lee
Ki Suk Han
Keun Bae Park
Jae Hyun Jung
Original Assignee
Sewavision Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sewavision Co., Ltd. filed Critical Sewavision Co., Ltd.
Publication of WO2009091168A2 publication Critical patent/WO2009091168A2/fr
Publication of WO2009091168A3 publication Critical patent/WO2009091168A3/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier

Definitions

  • the present invention relates to a method for restoring contaminated soil using microorganisms to decompose harmful substances, and more particularly, to restore contaminated soil such as oil by culturing microorganisms at high concentrations and mixing inorganic porous bodies carrying high concentration microorganisms with contaminated soils. It's about how to do it.
  • Chemical treatment is a method of stabilizing contaminants by injecting chemicals into contaminated soil. This method is applicable to contaminated soil sites, but has the disadvantage of damaging biological control of soil by adding chemicals.
  • Thermal treatment is a method to remove highly volatile organic contaminants by applying heat within a temperature not exceeding 600 ° C. Thermal treatment is mainly performed using rotary kiln. The disadvantage is that the soil is biologically dead.
  • Vacuum-extraction is a method to volatilize contaminants by treating the contaminated soil in a vacuum. This method is widely used to treat contaminated soil, especially near auto repair shops, contaminated with fuel or oil.
  • Vitrification is a method of vitrifying soils by treating contaminated soil at high temperature (maximum treatment temperature 1600 °C). Although it can be applied when several treatment methods are not feasible, the energy consumption is very large and expensive.
  • Electrokinetic extraction is the movement and extraction of contaminants in soil and groundwater using electrokinetic phenomena such as electroosmotic and electro-ion transport induced by currents supplied to soil and groundwater.
  • electrokinetic phenomena such as electroosmotic and electro-ion transport induced by currents supplied to soil and groundwater.
  • groundwater in the soil is moved to the cathode by electroosmosis, and at this time, the conditions that are applicable to the technique using the phenomenon of simultaneously adsorbing the soil or existing in the pore water also have limitations. .
  • the inorganic porous body carrying microorganisms having pollution decomposing ability is minimized to minimize the long-term pollution restoration period, and the most effective application conditions are formed. It suggests a method for restoring contaminated soil.
  • the present invention is to solve the problem that the secondary damage occurs or the soil is biologically dying to harm the biological adjustment of the soil in the restoration process of the contaminated soil, and to provide a method for restoring contaminated soil nature friendly.
  • the present invention aims to provide a method for restoring a contaminated soil that maximizes its efficiency in biological treatment and shortens the long-term pollution restoration treatment time.
  • the present invention by activating the microorganisms having the ability to decompose pollutants, carbon (C: Carbon), nitrogen (N: nitrogen), phosphorus (P: phosphorus) and the inorganic porous body on which the microorganisms are supported
  • the method is a method for restoring contaminated soil, characterized in that biologically decomposing contaminants of contaminated soil by mixing the inorganic porous body and the contaminated soil supported by the microorganism.
  • the contaminated soil and the inorganic porous body on which the microorganisms are loaded are stored in respective containers (Hopper) for drawing out separate amounts, and the inorganic porous body on which the contaminated soil and the microorganisms are loaded through the container.
  • Hopper containers for drawing out separate amounts
  • the inorganic porous body on which the contaminated soil and the microorganisms are loaded are loaded through the container.
  • Are each withdrawn at a predetermined ratio so that the contaminated soil and the inorganic porous body on which the microorganisms are loaded may be mixed.
  • the third step may further supply the liquid microbial preparation or nutritional components necessary for the survival of the microorganism through the sparging device.
  • the air and water injecting apparatus is installed in the place where the contaminated soil is excavated, and the soil contaminated with the inorganic porous body in which the microorganisms are loaded is refilled in the place where the contaminated soil is excavated.
  • the method may further include installing a top cover to prevent infiltration of foreign substances such as temperature control and excessive moisture from the outside.
  • the excavated contaminated soil and the inorganic porous body in which the microorganisms are loaded are mixed at a predetermined ratio and loaded at a predetermined ratio, and loaded in a range of 1 to 5 m.
  • the method may further include periodically stirring the soil.
  • the excavated contaminated soil and the inorganic porous body in which the microorganisms are loaded are loaded at a predetermined ratio in a separate place where water and an air injector are installed, and loaded at a rate of 1 to 5 m.
  • the step may further include supplying moisture and air to soil loaded periodically through the moisture and air injector.
  • the mixing moisture content of the organic material and the inorganic porous body is 20 to 75 wt%, and the supported microorganism concentration is 10 5 to 10 12 cfu / g.
  • the inorganic porous body preferably has a pH of 6 ⁇ 10.5.
  • the present invention it is possible to solve the problem that the secondary damage that damages the biological adjustment of the soil or the soil is biologically dead, it is possible to provide a method for restoring the soil contaminated nature.
  • the period for restoring the contaminated soil is reduced by using the inorganic porous body loaded with microorganisms.
  • Figure 1 shows a schematic flowchart of a method for restoring contaminated soil using the inorganic porous body loaded with microorganisms according to the present invention.
  • Figure 2 shows a schematic block diagram of a system for restoring contaminated soil using the inorganic porous body loaded with microorganisms according to the present invention.
  • 4 and 5 are graphs showing the results of experiments on the characteristics of the soil after mixing the porous body in the soil according to the present invention.
  • Figure 1 is a porous microorganism loaded with a microorganism according to the present invention Shows a schematic flow diagram for a method for restoring contaminated soil using.
  • an inorganic porous body is prepared using a siliceous raw material and a lime raw material (S110), and the inorganic porous body is loaded with nutrients (S120), and then the decomposition ability of the contaminants in the inorganic porous body is measured. Carry the microorganism with (S130).
  • the soil to be restored in the contaminated area is excavated (S140), and the inorganic porous body on which the microorganisms are supported and the contaminated soil excavated are mixed at a predetermined ratio (S150).
  • the mixed moisture content of the nutritional substance and the inorganic porous body is 20 to 75 wt%, and the concentration of the microorganism to be supported is 10 5 to 10 12 cfu / g. Also, the inorganic porous body is made to have a pH of 6 ⁇ 10.5 as possible.
  • the microorganism-supported inorganic porous body and the excavated contaminated soil are stored in a separate container (Hopper) 200a and 200b for quantitative extraction, and the contaminated soil and the contaminated soil through the containers 200a and 200b.
  • the inorganic porous body on which microorganisms are supported may be taken out and mixed at a predetermined ratio.
  • the contaminated soil mixed with the porous body may be buried again in an excavated place or loaded in a separate place at 1 to 5 m, in order to maintain the temperature of the loaded contaminated soil and prevent penetration of other impurities or rainwater.
  • a cover may be installed on top of the loaded soil.
  • a device for supplying air and moisture to the place where the contaminated soil is to be loaded in advance can be installed.
  • liquid microbial agents may be additionally supplied through the device to speed up soil remediation.
  • the polluted soil mixed with the porous body may be agitated periodically so that the nutrient, moisture and air can be spread evenly.
  • Figure 2 shows a schematic block diagram of a system for restoring contaminated soil using the inorganic porous body loaded with microorganisms according to the present invention.
  • the system for restoring contaminated soil using the inorganic porous body loaded with microorganisms includes a container 200a, carbon (C: Carbon), and nitrogen (N) for quantitatively extracting contaminated soil 100 to be restored as shown in FIG. 1. : nitrogen), vessel 200b for quantitatively extracting the inorganic porous body 500 loaded with phosphorus (P: phosphorus) and microorganisms, spraying device 400 for supplying nutrients for microorganisms, water, and contamination And a mixer 300 for evenly mixing the soil and the porous body.
  • the spreading device 400 may supply the liquid microbial agent to the contaminated soil in which the porous body is mixed.
  • the contaminated soil is embedded in a place where the contaminated soil is excavated again to restore the contaminated soil or the mixer 300 in a separate place.
  • the microorganisms are loaded with the porous body 500 and the contaminated soil 100, and then loaded, and periodically supply air and moisture to the loaded contaminated soil, or periodically contaminate through a machine such as a tiller. Soil can be restored.
  • a cover may be installed on top of the embedded soil or loaded soil.
  • the inorganic porous body may be manufactured by hydrothermal reaction using a foaming agent and an air bubble stabilizer in a siliceous raw material and a lime raw material, which are various descriptions other than as a description of one embodiment.
  • An inorganic porous body may be produced by the method.
  • the inorganic porous body prepared in this way is mixed with a high concentration of organic material, and is fermented to prepare an inorganic porous body supporting nutrients.
  • Table 1 below shows the amount of nutrients loaded in the porous body that can be used in the present invention.
  • Organic nutrients may be used in various ways, the organic material of Table 1 is disclosed as an embodiment in the present invention, but is not limited thereto.
  • the total nitrogen content (TN), the total phosphorus content (TP) and the ratio of carbon to nitrogen (C / N) and the respective pH values were calculated as the mass percentage of each target organic material. It is used as a substance to produce a porous body loaded with nutrients.
  • the microorganism When a porous body on which such a nutrient is loaded is prepared, the microorganism is supported on the porous body, and the microorganism is cultured in the porous body.
  • an inorganic porous body in which microorganisms are supported is manufactured.
  • Table 2 shows the conditions for experimenting with the same initial oil concentration of the examples according to the present invention and the examples of injecting liquid microorganisms as a general prior art.
  • the initial oil concentration is 22,000 ppm and the nutritional component C: N: P is 100: 10: 1, and the soil restoration method according to the present invention and the soil restoration method according to the prior art are performed under the same conditions. .
  • the microorganisms are loaded on the inorganic porous material and mixed with the contaminated soil.
  • the concentration of microorganisms per contaminated soil is 1.7x10 6 cfu / g ⁇ soil and the microorganisms on the contaminated soil are supported.
  • the mass percentage of the porous body was formed at 5 wt%.
  • the second embodiment and the third are each the concentration of microorganisms per contaminated soil 3.2x10 6 cfu / g ⁇ soil and 4.4x10 6 cfu / g ⁇ soil, the mass percentage of the inorganic porous body microorganisms supported on the contaminated soils are each 10 wt% and 15 wt% were formed.
  • the initial oil concentration which is substantially the same condition as the example according to the present invention, was formed at a condition of 22,000 ppm and the nutrient component C: N: P degree of 100: 10: 1,
  • experiments were conducted by forming conditions at 10 6 cfu / g ⁇ soil, which are almost similar in concentrations of microorganisms per contaminated soil.
  • the population of microorganisms is rapidly increased by supporting the microorganisms in the inorganic porous body and mixed with the contaminated soil, so that the contaminated soil can be restored within a faster period than the conventional art.
  • the efficiency is increased.
  • This effect is caused by mixing the inorganic porous body loaded with microorganisms with the contaminated soil, as in the present invention, and the inorganic porous body has better water retention than ordinary soils, so it absorbs more water and also has less amount of outflowed water. do.
  • the moisture retention capacity of the inorganic porous body can keep a large amount of water necessary for long-term microbial activity for a long time, and since the porous body absorbs moisture generated when microorganisms decompose contaminants such as oil, the effect of suppressing leachate is generated. .
  • the inorganic porous body according to the present invention has sufficient nutrients such as C, N, P necessary for microbial metabolism, and the contaminated soil in which the porous body is mixed due to the porous body increases porosity, thereby smoothly supplying oxygen.
  • the number of microorganisms decomposing contaminants rapidly increases over time, thereby maximizing the restoration efficiency of the contaminated soil.
  • the y-axis of FIG. 3 represents the total petroleum hydrocarbon (THP) on a log scale, and the x-axis represents the course of days.
  • THP total petroleum hydrocarbon
  • K -0.0034
  • the restoration method of the contaminated soil using the microorganism-supported inorganic porous body according to the present invention can restore the contaminated soil in a much faster time than the conventional method of restoring contaminated soil according to the prior art, and thus, the efficiency of restoring contaminated soil It can be seen that this is high.
  • 4 and 5 are graphs showing the results of experiments on the characteristics of the soil after mixing the inorganic porous body in the soil.
  • the clay was used as clay soil, and the experiment was carried out using a sample formed by passing a sieve of 2.36 mm and having a water content of 2 wt% or less.
  • 5 is a graph for improving the water holding capacity in the soil, where the y-axis shows the permeation rate and the x-axis shows the amount of the inorganic porous body.
  • the permeation rate is much faster than the permeation rate of the soil mixed with the inorganic porous body in the case of the soil without mixing the inorganic porous body, and the permeation rate is lowered as the amount of the inorganic porous body is increased. You can check it.
  • the inorganic porous body has better water retention than the general soil, so the soil mixed with the porous body may contain much more water than when only the soil exists.
  • the soil mixed with the inorganic porous body has better air permeability and a lower permeation rate than the soil without the soil.
  • the soil mixed with the inorganic porous body can supply the nutrients evenly to the microorganisms spread throughout and increase the water content of the soil, thereby obtaining much more effective results.

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Soil Sciences (AREA)
  • Environmental & Geological Engineering (AREA)
  • Processing Of Solid Wastes (AREA)
  • Soil Conditioners And Soil-Stabilizing Materials (AREA)

Abstract

L'invention concerne un procédé destiné à réhabiliter un sol pollué à l'aide d'un corps poreux inorganique. L'invention est caractérisée en ce que le corps poreux inorganique activé par du carbone (C), de l'azote (N), du phosphore (P) et des microorganismes, est fabriqué par activation desdits microorganismes qui présentent la capacité de dégrader les contaminants; et en ce que les contaminants du sol pollué sont biodégradés par mélange du corps poreux inorganique activé par les microorganismes et du sol pollué. Selon l'invention, une période de réhabilitation du sol pollué est raccourcie grâce à l'utilisation du corps poreux inorganique activé par les microorganismes, dans le but de résoudre les problèmes concernant une réhabilitation à long terme du sol pollué, qui surviennent pendant un traitement biologique classique.
PCT/KR2009/000165 2008-01-16 2009-01-13 Procédé destiné à réhabiliter un sol pollué à l'aide d'un corps poreux inorganique WO2009091168A2 (fr)

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KR20080005023A KR100992505B1 (ko) 2008-01-16 2008-01-16 무기질 다공체를 이용한 오염토양 복원 방법
KR10-2008-0005023 2008-01-16

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WO2009091168A2 true WO2009091168A2 (fr) 2009-07-23
WO2009091168A3 WO2009091168A3 (fr) 2009-10-22

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111440741A (zh) * 2020-04-03 2020-07-24 中国科学院沈阳应用生态研究所 一种适用于直流电场阳极区中石油污染土壤修复的功能菌及应用

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170016223A (ko) 2015-08-03 2017-02-13 현대엔지니어링 주식회사 오염토양 정화용 조성물 및 이를 이용한 생물학적 원위치 오염토양 정화방법
KR101896424B1 (ko) * 2016-12-29 2018-10-18 이균 다공성 미네랄 제조방법 및 이를 이용한 수용화된 다공성 미네랄 조성물 제조방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0646642A2 (fr) * 1993-09-30 1995-04-05 Canon Kabushiki Kaisha Support des microorganismes et méthode pour l'amélioration du sol en utilisant ce support
KR20030073555A (ko) * 2002-03-12 2003-09-19 동양물산기업 주식회사 미생물 고정화용 다공성 세라믹 담체
JP2004148212A (ja) * 2002-10-30 2004-05-27 Okumura Engineering Corp 汚染土壌用浄化材、及びそれを用いた浄化方法
KR20050080651A (ko) * 2004-02-10 2005-08-17 에스케이 주식회사 유효미생물의 토양집적에 의한 유류오염토양의 생물학적복원방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0646642A2 (fr) * 1993-09-30 1995-04-05 Canon Kabushiki Kaisha Support des microorganismes et méthode pour l'amélioration du sol en utilisant ce support
KR20030073555A (ko) * 2002-03-12 2003-09-19 동양물산기업 주식회사 미생물 고정화용 다공성 세라믹 담체
JP2004148212A (ja) * 2002-10-30 2004-05-27 Okumura Engineering Corp 汚染土壌用浄化材、及びそれを用いた浄化方法
KR20050080651A (ko) * 2004-02-10 2005-08-17 에스케이 주식회사 유효미생물의 토양집적에 의한 유류오염토양의 생물학적복원방법

Non-Patent Citations (1)

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Title
CHO DAE-CHUL ET AL.: 'Remediation ofPAH-Polluted Soil by Pseudomonas sp. Adhered on PU Foam' JOURNAL OF THE KOREAN ACADEMIC INDUSTRIAL SOCIETY vol. 7, no. 3, 2006, pages 458 - 464 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111440741A (zh) * 2020-04-03 2020-07-24 中国科学院沈阳应用生态研究所 一种适用于直流电场阳极区中石油污染土壤修复的功能菌及应用
CN111440741B (zh) * 2020-04-03 2022-05-31 中国科学院沈阳应用生态研究所 一种适用于直流电场阳极区中石油污染土壤修复的功能菌及应用

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KR20090079072A (ko) 2009-07-21
WO2009091168A3 (fr) 2009-10-22

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