WO2009083648A3 - Instrument de cyclage thermique et procédés pour conduire la pcr - Google Patents

Instrument de cyclage thermique et procédés pour conduire la pcr Download PDF

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Publication number
WO2009083648A3
WO2009083648A3 PCT/FI2008/050781 FI2008050781W WO2009083648A3 WO 2009083648 A3 WO2009083648 A3 WO 2009083648A3 FI 2008050781 W FI2008050781 W FI 2008050781W WO 2009083648 A3 WO2009083648 A3 WO 2009083648A3
Authority
WO
WIPO (PCT)
Prior art keywords
samples
sample
carrying
methods
spaces
Prior art date
Application number
PCT/FI2008/050781
Other languages
English (en)
Other versions
WO2009083648A2 (fr
Inventor
David A. Cohen
Michael J. Mortillaro
Charles Patrick André
Original Assignee
Finnzymes Instruments Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FI20075960A external-priority patent/FI20075960A0/fi
Priority claimed from US11/965,660 external-priority patent/US20090165574A1/en
Application filed by Finnzymes Instruments Oy filed Critical Finnzymes Instruments Oy
Priority to DE112008003552T priority Critical patent/DE112008003552T9/de
Publication of WO2009083648A2 publication Critical patent/WO2009083648A2/fr
Publication of WO2009083648A3 publication Critical patent/WO2009083648A3/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/142—Preventing evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates

Abstract

La présente invention concerne un processus de réaction de polymérase en chaîne (PCR) et un cycleur thermique. Dans le procédé, des échantillons biologiques sont contenus dans un porte-échantillon ayant une pluralité d'espaces d'échantillon ayant chacun des extrémités supérieure et inférieure et les échantillons sont séquentiellement chauffés et refroidis. Le cycleur thermique selon l'invention comprend des moyens de transfert thermique pour le chauffage et le refroidissement automatiques des échantillons dans le porte-échantillon, des moyens de fermeture pouvant être chauffés au-dessus des extrémités supérieures des espaces d'échantillon pour prévenir la condensation de vapeur d'échantillon pendant le processus, et des moyens pour ajuster un paramètre de processus, de préférence la température des moyens de fermeture pouvant être chauffés, en fonction d'au moins un autre desdits paramètres de processus. L'invention contribue à diminuer le nombre d'essais PCR défaillants, en particulier en raison de changements de volume d'échantillon.
PCT/FI2008/050781 2007-12-27 2008-12-22 Instrument de cyclage thermique et procédés pour conduire la pcr WO2009083648A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE112008003552T DE112008003552T9 (de) 2007-12-27 2008-12-22 Thermozykler-Instrument und Verfahren zur Durchführung von PCR

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11/965,660 2007-12-27
FI20075960A FI20075960A0 (fi) 2007-12-27 2007-12-27 Uusi laite ja menetelmä
US11/965,660 US20090165574A1 (en) 2007-12-27 2007-12-27 Instrument and method for nucleic acid amplification
FI20075960 2007-12-27

Publications (2)

Publication Number Publication Date
WO2009083648A2 WO2009083648A2 (fr) 2009-07-09
WO2009083648A3 true WO2009083648A3 (fr) 2009-09-03

Family

ID=40642088

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI2008/050781 WO2009083648A2 (fr) 2007-12-27 2008-12-22 Instrument de cyclage thermique et procédés pour conduire la pcr

Country Status (3)

Country Link
DE (1) DE112008003552T9 (fr)
GB (1) GB2468993A (fr)
WO (1) WO2009083648A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8252581B2 (en) 2007-01-22 2012-08-28 Wafergen, Inc. Apparatus for high throughput chemical reactions
US9228933B2 (en) 2004-05-28 2016-01-05 Wafergen, Inc. Apparatus and method for multiplex analysis

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6620160B2 (ja) 2015-02-20 2019-12-11 タカラ バイオ ユーエスエー， インコーポレイテッド 単一細胞の迅速かつ正確な分注、視覚化及び解析のための方法
EP3487616B1 (fr) 2016-07-21 2023-08-09 Takara Bio USA, Inc. Imagerie multi-z des puits de dispositifs multipuits et distribution de liquide dans les puits
CN114367326A (zh) * 2022-01-11 2022-04-19 无锡科智达科技有限公司 一种带精密温度补偿的变温模块及补偿方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
EP1409137A2 (fr) 2001-07-13 2004-04-21 University of British Columbia Procedes et appareils de cyclage thermique
US6730883B2 (en) 2002-10-02 2004-05-04 Stratagene Flexible heating cover assembly for thermal cycling of samples of biological material

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BIO-RAD LABORATORIES, INC.: "MJ Mini Gradient Thermal Cycler. Operations Manual", 2005, pages 1 - 96, XP002530917, Retrieved from the Internet <URL:http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_10968.pdf> [retrieved on 20090614] *
FINNZYMES INSTRUMENT: "Piko Thermal Cycler. User Manual.", October 2007 (2007-10-01), pages 1 - 14, XP002530918, Retrieved from the Internet <URL:http://www.finnzymes.com/pdf/piko_thermal_cycler_usermanual.pdf> [retrieved on 20090614] *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9228933B2 (en) 2004-05-28 2016-01-05 Wafergen, Inc. Apparatus and method for multiplex analysis
US8252581B2 (en) 2007-01-22 2012-08-28 Wafergen, Inc. Apparatus for high throughput chemical reactions
US9132427B2 (en) 2007-01-22 2015-09-15 Wafergen, Inc. Apparatus for high throughput chemical reactions

Also Published As

Publication number Publication date
DE112008003552T5 (de) 2010-10-28
GB2468993A (en) 2010-09-29
DE112008003552T9 (de) 2011-03-10
WO2009083648A2 (fr) 2009-07-09
GB201012081D0 (en) 2010-09-01

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