WO2009072006A2 - A centrifuge for separating a sample into at least two components - Google Patents
A centrifuge for separating a sample into at least two components Download PDFInfo
- Publication number
- WO2009072006A2 WO2009072006A2 PCT/IB2008/003845 IB2008003845W WO2009072006A2 WO 2009072006 A2 WO2009072006 A2 WO 2009072006A2 IB 2008003845 W IB2008003845 W IB 2008003845W WO 2009072006 A2 WO2009072006 A2 WO 2009072006A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sample
- chamber
- separation
- centrifuge
- progress
- Prior art date
Links
- 238000000926 separation method Methods 0.000 claims abstract description 41
- 239000000523 sample Substances 0.000 claims description 107
- 238000005119 centrifugation Methods 0.000 claims description 31
- 210000004027 cell Anatomy 0.000 claims description 20
- -1 polysterene Polymers 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 10
- 229920002223 polystyrene Polymers 0.000 claims description 8
- 239000004698 Polyethylene Substances 0.000 claims description 6
- 239000004433 Thermoplastic polyurethane Substances 0.000 claims description 6
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 6
- 229920000573 polyethylene Polymers 0.000 claims description 6
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 6
- 229920002803 thermoplastic polyurethane Polymers 0.000 claims description 6
- 238000005253 cladding Methods 0.000 claims description 5
- 210000001185 bone marrow Anatomy 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 229920000058 polyacrylate Polymers 0.000 claims description 4
- 229920000515 polycarbonate Polymers 0.000 claims description 4
- 239000004417 polycarbonate Substances 0.000 claims description 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical class CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 2
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims description 2
- 229920002101 Chitin Polymers 0.000 claims description 2
- 102000008186 Collagen Human genes 0.000 claims description 2
- 108010035532 Collagen Proteins 0.000 claims description 2
- 229940072056 alginate Drugs 0.000 claims description 2
- 235000010443 alginic acid Nutrition 0.000 claims description 2
- 239000012472 biological sample Substances 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 claims description 2
- 239000008280 blood Substances 0.000 claims description 2
- 239000001506 calcium phosphate Substances 0.000 claims description 2
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 2
- 235000011010 calcium phosphates Nutrition 0.000 claims description 2
- 230000001413 cellular effect Effects 0.000 claims description 2
- 239000000919 ceramic Substances 0.000 claims description 2
- 229920001436 collagen Polymers 0.000 claims description 2
- 229920001577 copolymer Polymers 0.000 claims description 2
- 229920003023 plastic Polymers 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- 229920001296 polysiloxane Polymers 0.000 claims description 2
- 239000004800 polyvinyl chloride Substances 0.000 claims description 2
- 229920000915 polyvinyl chloride Polymers 0.000 claims description 2
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 2
- 229920000615 alginic acid Polymers 0.000 claims 1
- 238000012545 processing Methods 0.000 description 39
- 238000001514 detection method Methods 0.000 description 9
- 239000012530 fluid Substances 0.000 description 9
- 238000004113 cell culture Methods 0.000 description 8
- 239000007789 gas Substances 0.000 description 8
- 239000012528 membrane Substances 0.000 description 8
- 230000003287 optical effect Effects 0.000 description 7
- 230000005540 biological transmission Effects 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 239000006143 cell culture medium Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000007885 magnetic separation Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011038 discontinuous diafiltration by volume reduction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 239000013020 final formulation Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/461—Cellular immunotherapy characterised by the cell type used
- A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/461—Cellular immunotherapy characterised by the cell type used
- A61K39/4615—Dendritic cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/462—Cellular immunotherapy characterized by the effect or the function of the cells
- A61K39/4621—Cellular immunotherapy characterized by the effect or the function of the cells immunosuppressive or immunotolerising
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/462—Cellular immunotherapy characterized by the effect or the function of the cells
- A61K39/4622—Antigen presenting cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
- A61K39/4643—Vertebrate antigens
- A61K39/46433—Antigens related to auto-immune diseases; Preparations to induce self-tolerance
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
- A61K39/4643—Vertebrate antigens
- A61K39/46434—Antigens related to induction of tolerance to non-self
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
- A61K39/4643—Vertebrate antigens
- A61K39/4644—Cancer antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
- A61K39/464838—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/02—Blood transfusion apparatus
- A61M1/0209—Multiple bag systems for separating or storing blood components
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3618—Magnetic separation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3621—Extra-corporeal blood circuits
- A61M1/3627—Degassing devices; Buffer reservoirs; Drip chambers; Blood filters
- A61M1/3633—Blood component filters, e.g. leukocyte filters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3679—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits by absorption
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3692—Washing or rinsing blood or blood constituents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
- A61M1/3696—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3861—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36 using an external stimulus
- B01D15/3885—Using electrical or magnetic means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D21/00—Separation of suspended solid particles from liquids by sedimentation
- B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/002—High gradient magnetic separation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/027—High gradient magnetic separators with reciprocating canisters
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/031—Component parts; Auxiliary operations
- B03C1/032—Matrix cleaning systems
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/031—Component parts; Auxiliary operations
- B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
- B03C1/0332—Component parts; Auxiliary operations characterised by the magnetic circuit using permanent magnets
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/031—Component parts; Auxiliary operations
- B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
- B03C1/0335—Component parts; Auxiliary operations characterised by the magnetic circuit using coils
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/031—Component parts; Auxiliary operations
- B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
- B03C1/034—Component parts; Auxiliary operations characterised by the magnetic circuit characterised by the matrix elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B1/00—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles
- B04B1/10—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles with discharging outlets in the plane of the maximum diameter of the bowl
- B04B1/12—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles with discharging outlets in the plane of the maximum diameter of the bowl with continuous discharge
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B13/00—Control arrangements specially designed for centrifuges; Programme control of centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/10—Centrifuges combined with other apparatus, e.g. electrostatic separators; Sets or systems of several centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
- B04B7/08—Rotary bowls
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0646—Natural killers cells [NK], NKT cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0669—Bone marrow stromal cells; Whole bone marrow
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3621—Extra-corporeal blood circuits
- A61M1/3623—Means for actively controlling temperature of blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/0071—Special media to be introduced, removed or treated product to be retained or harvested, e.g. by pheresis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/04—Liquids
- A61M2202/0413—Blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/04—Liquids
- A61M2202/0413—Blood
- A61M2202/0427—Platelets; Thrombocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/10—Bone-marrow
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D21/00—Separation of suspended solid particles from liquids by sedimentation
- B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
- B01D21/262—Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
- B04B2005/0464—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation with hollow or massive core in centrifuge bowl
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B13/00—Control arrangements specially designed for centrifuges; Programme control of centrifuges
- B04B2013/006—Interface detection or monitoring of separated components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/998—Proteins not provided for elsewhere
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705
Definitions
- the invention relates to a centrifuge for separating a sample into at least two components and to a method for separating a sample into at least two components in which this centrifuge is used.
- centrifugation can be used in a physical method to separate a sample into at least two of its components.
- centrifugation is used in the art to separate samples of biological origin into two or more components. These components can then separately be further processed.
- the problem underlying the present invention was to provide a means of performing a centrifugation that allowed for monitoring the progress of the separation process.
- the problem underlying the present invention is solved both by a centrifuge and a method of using this centrifuge, both as described herein.
- a centrifuge for separating a sample into at least two components.
- a centrifuge comprises a chamber (or processing chamber or rotating container) for receiving a sample to be centrifuged and a means for controlling the progress of the sample separation located at the chamber.
- the saparation of the sample results in at least a first component and a second component that are separated from each other.
- the components therefore form layers in the centrifuge chamber that can be detected.
- the means for controlling the progress of the sample separation is a window, a mirror or a prism that is located such that light from a light source can be transmitted through at least a part of the sample and that the light leaving the prism is detectable by a light detector.
- the means for controlling the progress of the sample separation can be a double prism, with two prism sections aligned in a mirror symmetrical fashion.
- the chamber of the centrifuge comprises a circular base plate, the center of which is oriented substantially perpendicular to a rotational axis; a cladding or wall which is oriented substantially perpendicular to the base plate such that base plate and cladding together form a pot-like structure (lower portion of the chamber); and a circular cover plate (lid; upper portion of the chamber), which is positionable on the edge of the cladding that is remote from or opposite the base plate and the center of which is oriented substantially perpendicular to the rotational axis.
- a closed centrifugation chamber is formed, consisting of a pot-like bottom part and an upper part in the form of a lid.
- the means for controlling the progress of the sample separation is positioned at the base plate or the cover plate of the chamber. It is preferred that the means for controlling the progress of the sample separation (e.g. the prism or double prism) is positioned at the cover plate of the chamber.
- the means for controlling the progress of the sample separation is preferably positioned at a channel or at a gap which is located in the base plate or the cover plate of the chamber such that the sample can enter the channel or gap during the centrifugation such that the sample becomes detectable.
- the channel or gap is configured such that at least a part of the sample can flow into it during centrifugation.
- the separation of the sample becomes detectable, since light can at least in part penetrate the different components of the sample. Thereby, a signal is generated that allows for determining when the sample separation is complete.
- the chamber can comprise an outlet opening that allows for a component of the sample to be drained from the chamber during centrifugation.
- the saparation of the sample can also be detected using the pH value and/or temperature. This will be explained in more detail with reference to the figures.
- the channel is oriented such that it stretches radially in a linear fashion from an area located at the rotation axis to an area located at a perimeter of the base plate or the cover plate, depending on where the detection means is located.
- the channel is positioned such that at either side light can pass from the prism or double prism into the channel which holds sample during centrifugation.
- the detection of the light passing through the sample allows for determining how far the separation of the sample has proceeded and also for determining the position of the borders between different components of the sample. Based on the knowledge of the position of the borders between different components, it becomes possible to drain certain components from the chamber through at least one outlet port, which is located at the chamber.
- the chamber is configured such that it can serve as or accommodate a container for the cultivation of cells.
- the centrifugation chamber can be used both for cell culture purposes and for processing of the cells grown therein.
- the chamber allows a large range of cell culture methods to be performed, such as growing of cells, separating, washing, enriching the cells or different kinds of cells, or others.
- the chamber may comprise further inlet/outlet openings, e.g. for gas, cell culture media or alike. Cell culture conditions are known in the art.
- a method for separating a sample into at least two components comprises the steps of providing a sample, and centrifuging the sample in a centrifuge as described above and herein.
- the sample is preferably a biological sample, such as blood, bone marrow, cells, compositions comprising cells or cellular components or alike.
- the centrifuge can be part of a sample processing unit.
- This sample processing unit may comprise an input port and an output port coupled to centrifuge chamber as described herein having at least one sample chamber, wherein the sample processing unit is configured to provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample.
- the sample processing unit can be coupled to a sam- pie separation unit to form a system.
- the sample separation unit can be coupled to the output port of the sample processing unit, wherein the sample separation unit comprises a separation column holder, a pump, and a plurality of valves configured to at least partially control fluid flow through a fluid circuitry and a separation column positioned in the holder, wherein the separation column is configured to separate labeled and unlabeled components of sample flowed through the column.
- the sample separation unit comprises a separation column holder, a pump, and a plurality of valves configured to at least partially control fluid flow through a fluid circuitry and a separation column positioned in the holder, wherein the separation column is configured to separate labeled and unlabeled components of sample flowed through the column.
- Figure 1 illustrates a chamber of a centrifuge, according to an embodiment of the present invention.
- Figure IA illustrates a chamber of a centrifuge according to an embodiment of the present invention with a light source and a detector for detecting the progress of the sample separation in the chamber.
- Figure 2 shows a cross-sectional view of a processing chamber of a centrifuge, according to an embodiment of the present invention.
- Figure 2A illustrates a top plan view of a processing chamber, according to an embodiment of the present invention.
- Figure 3 shows a cross-sectional view of a processing chamber, according to another embodiment of the present invention.
- Figure 3A Illustrates a focused view of a portion of a processing chamber as shown in Figure
- Figure 4 shows a cross-sectional view of a processing chamber, according to yet another embodiment of the present invention.
- Figure 4 A shows a view over the bottom of the process chamber with opening for gas delivery and a bonded hydrophobic membrane.
- Figure 4B shows an embodiment of the bottom of the chamber with spiral channels for aeration through membrane bonded to the bottom of the chamber.
- Figure 5 shows a view of the inside of a lid for a rotating chamber with a channel or gap in which the sample flows during centrifugation, with a means for detecting the progress of separation of the sample in the form of a prism.
- Figure 6 shows the path of light through the sample by means of a prism.
- the prism double prism is configured such that light from a light source can at least partially penetrate through at least a part of the sample that is being separated through centrifugation, and light passing through at least a part of the sample can be detected by a light detector.
- Figure 7 shows a double prism that is part of a rib located at the lid of the rotating chamber.
- Figure 8 illustrates a system with a centrifuge according to an embodiment of the present invention.
- the present invention provides a centrifuge for separating a sample into at least two components.
- a centrifuge comprises a chamber or processing chamber for receiving a sample to be centrifuged and a means for controlling the progress of the sample separation is located at the chamber.
- sample chamber or chamber which may also be part of a processing unit (see below), is now further described with reference to Figures 1 through 4.
- the chamber 170 includes an upper portion 172 and a lower portion 174, with a rotational axis 176 and fluid ports or line connect 176, 178 fluidly connected to one or more internal compartments of the chamber 170.
- the upper portion 172 includes a support structure 182 that is oriented substantially radially, stretching from an area located at the rotation axis 176 to an area located at a perimeter of the upper portion 172, as well as structure 184 including a channel 186 that can include at least a portion that is visible through a window or prism 188.
- the channel 186 can be fluidly coupled to a sample containment compartment in the chamber 170 and configured for external monitoring or detection of sample processing.
- a component e.g., cells
- Rotational means or bearing 180 provides rotational movement of the chamber 170 about the axis 176.
- the chamber 170 may further comprise at least one vent, preferentially comprising a sterile, hydrophobic membrane or tampon.
- these membranes or tampons may be located at the top or bottom of the chamber.
- the at least one vent in the chamber has the particular advantage that the volume in the chamber can be changed easily without changing the pressure in the chamber or providing further inlet and/or outlet ports for the exchange of air or gas.
- the centrifuges known in the art allow batch-wise centrifugation, i.e. if the volume of the sample to be reduced or concentrated is larger than the chamber, several centrifugation steps are necessary to receive the concentrated product.
- the system allows continuous centrifugation: sample, media, gases and other materials can enter and leave the system e.g. through inlet and outlet ports (e.g. Fig. 1: inlet port 178 and outlet port 180) without stopping the centrifugation process and refilling the centrifuge (batch- wise centrifugation). This allows a continuous concentration of the sample and the product may be removed only once at the end of the centrifugation, thus avoiding potential contamination due to additional handling.
- a rotating container or centrifugation chamber 500 is shown.
- a microscope focus area 505 is positioned which comprises at least one sensor pad 504.
- a microscope camera module 503 is located below the rotating chamber 500 that comprises a microscope optics 501 and a microscope drive motor 502 for focusing the optics.
- the microscope optics 501 is configured such that is can focus automatically to detect the sample that is being separated into at least two components during centrifugation. Thereby, the microscope camera module 503 can be used to detect different layers formed by the separated sample in the chamber 500 due to centrifugal forces.
- the pH value of the sample components can be measured.
- an indicator is used in the chamber 500 that changes its color dependent on the pH value that is present.
- the temperature of the sample in the chamber be measured using liquid crystals that are position in the chamber such that their position can be detected with a microscope camera module 503 from the outside. Thereby, the temperature in the chamber 500 can be determined.
- the microscope camera module 503 can be mounted in a movable fashion, such that the mod- ule 503 can be directed with its microscope optics 501 at different sensor pads 504 located in the wall of the chamber 500. This facilitates the detection of various layers formed in the chamber 500 or the detection of the pH or the temperature at different positions within the chamber 500.
- FIG. 2 illustrates a cross-sectional view of a sample processing chamber according to an embodiment of the present invention.
- the chamber 190 includes an upper portion 192 and a base portion 194, and one or more internal compartments.
- the chamber 190 is configured to rotate about an axis so as to apply a centrifugal force to sample disposed in one or more compartments ' in the chamber, thereby separating at least two components of the sample.
- the chamber includes central line 196 fluidly connected to at least one compartment of the chamber.
- Components of the chamber 190 further include outer line 198; rotational bearing 200, rotational seals 202, 204, 206; outer entry line to the chamber 205; lower radial channel 208; inner line entry 210 to a chamber compartment; slant 212, and deflector 214.
- Chamber retainer 216 is included and configured for secure positioning/coupling of the chamber 190 with other components of a system of the invention.
- the centrifugation chamber 190 preferably comprises a rotating seal, optionally with two fluid lines, preferably with two fluid lines.
- the fluid lines can enter the chamber 190 at different position. For example, it is possible to position a first fluid line at the outer perimeter of the upper portion 192 (lid). A second fluid line could be positioned further inward, e.g. 2 mm to 20 mm further towards the center of the chamber 190.
- a vent can be located at the upper portion 192, e.g. in the form of a membrane.
- the position of openings such as holes or line entries in the centrifugation chamber can be configured such that they are best suited for the centrifugation of a particular sample.
- the openings can be positioned so that the removal and/or detection of a particular component can be achieved.
- Figure 2 A illustrates a top plan view of a chamber 201.
- the chamber 201 includes an inner line 203, lower radial channel 205, inner line entry 207 to the chamber, optionally a deflector 209, slant 211 and a light pass.
- Figure 3 illustrates a cross-sectional view of a chamber according to another embodiment of the present invention.
- the chamber 220 includes an axis about which the chamber rotates, a central line connect 222 and a outer line connect 224, and one or more internal compartments. Further illustrated are rotational bearing 226, as well as rotational seals 228, 230, 232; inner channel 234, optical detection channel 236 (similar to described above); inner line entry 238 to the chamber; inner line 240, and lower radial channel 242.
- the chamber further includes an inner reinforcement 246 and a chamber retainer 248.
- Figure 3A illustrates a focused view of portion of a chamber 220 is described above.
- an optical detection channel 236, a prism 237, and light pass 239 Shown are an optical detection channel 236, a prism 237, and light pass 239 (further indicated by arrows). It can be seen that light from a light source (not shown) is directed by the prism 237such that is goes through the channel 234 which is filled with sample during centrifugation. The light exiting from the channel 234 is redirected by the prism 237 and can be detected by a detector (not shown).
- the bottom of the chamber of the centrifuge can possess one or more openings 291 that may be covered with a hy- drophobic membrane 292. These openings 291 are used for delivery of gases into the chamber, for instance for cell culture processes (such as CO 2 , N 2 , O 2 , etc.).
- the membrane can be glued, or bonded thermally or by ultrasound or by other means bonded to the bottom of the chamber in a way to assure sterile connection with the chamber.
- the bottom of the chamber can possess a system of channels for the gas flow, for instance channels assembled as a spiral system 293, which assures a large contact area between the gases and a membrane bonded over the channels (not shown).
- the channel system possesses at least one input (opening) 294 and an optional output (opening) 295 for the gases.
- the entries or ports of the channels of Figs. 1-4A may vary in number and location within the channel. ;
- Figure 4 illustrates a cross-sectional view of a chamber according to another embodiment of the present invention.
- Construction of chamber 250 is similar in many regards to chambers as described above, but further includes a plurality of layered structures 252.
- the layered structures 252 can be configured to provide cell culture structures or layers.
- sample including cells can be introduced into the chamber and flowed over layers 252.
- Separation process- ing can include rotation of the chamber such that cells adhering to the layers are separated from those with lesser affinity for the layers. Intermittent rotation and/or breaking during rotation can further disconnect cultured cells from the surface of the layered structures 252 for separation processing.
- the chamber further includes an illustrated central line 251 , outer line 253, bearing 255, rotational seals 257, outer line entry 259 to the chamber, upper portion 261, inner channel 263, base portion 265, retainer 267, lower radial channel 269, and inner line entry 271 to the chamber.
- the chamber as described herein may comprise or may be made of various materials.
- transparent materials are used like plastics, polystyrol (PS), polysterene, polyvinylchloride, polycarbonate, glass, polyacrylate, polyacrylamide, polymethylmethacrylate (PMMA), and/or polyethylenterephtala (PET).
- PS polystyrol
- PMMA polymethylmethacrylate
- PET polyethylenterephtala
- PTFE polytetrafluorethylen
- TPU thermoplastic polyurethane
- silicone silicone or compositions comprising one or more of the above mentioned materials.
- the chamber can also be made of polyethylene (PE).
- the layers in the chamber comprise or are made of collagen, chitin, algi- nate, and/or hyaluronic acid derivatives. Possible are also polyactide (PLA), olyglycolida (PGA) and their copolymers, which are biodegradable.
- PLA polyactide
- PGA olyglycolida
- non-biodegradable materials can be used, such as polystyrol (PS), polysterene, polycarbonate, polyacrylate, polyethylene (PE), polymethylmethacrylate (PMMA), and/or polyethylenterephtala (PET).
- PS polystyrol
- PTFE polytetrafluorethylen
- TPU thermoplastic polyurethane
- Other alternatives include ceramics and glass materials, like hydroxylapatite (HA) or calcium phosphate.
- the layers in the chamber can be of solid material or porous.
- the chamber has a size of 2 cm to 50 cm in diameter and a height of 5 mm to 50 cm. Centrifugation is preferentially carried out up to 1000 xg. The number of the layers and the distance between the layers is variable.
- the chamber can be heated and cooled to provide for a temperature appropriate for the sample to be centrifuged.
- a heating and/or cooling means can be located at the chamber or surrounding the chamber. The detection of optical layers in the centrifugal chambers is shown in detail in Figures 5-7.
- the cylindrical shaped centrifuge chamber shown in figure 5 is limited on its upper side by a lid 800, which may carry one or more some stabilizing ribs 805 on the flat top surface. At least one of these radial ribs 805 covers a narrow gap or channel 801, open to the centrifuges inner volume when the lid 800 is tied on the centrifuge chamber.
- the gap 801 extends in axial direction from the inner lid surface passing the lid 800 some millimeters into the rib 805.
- the gap 801 reaches from near the center up to the cylindrical centri- fuge wall (Fig. 5).
- the ring shaped neighbored suspension layers extend parallel into the gap 801 and are displayed as axial standing neighbored thin areas, like a thin layers cross cut, well detect- ible by external optical sensors.
- the gap 801 width can be determined freely, but need to be small enough for a transmitted light analysis of all layer-associated areas in the gap. Thereby, it is possible to quantify the optical densities and colors of all layers of the suspension in the centrifuge chamber in a "touchless" manner from the outside through optical transmission measurements.
- a prism can be added to a rib, e.g. on both rib sides, which may be preformed by the transparent housing material itself.
- the prism 810 refracts the vertical generated illuminating beam through the gap (horizontal) and back to the top, vertical again (Fig. 6).
- a synchronous position triggered electronic flash light can transmit light into one side of the prism 810, e.g. the left prism, illuminating the gap by refraction.
- the transmission result is refracted by the other side of the prism 810, e.g. the right prism, back to a vertical mounted camera, possibly in the neighborhood to the upper flash source on the top.
- the resulting optical sensor unit is easy to handle like a reflex sensor but at the same time allows for full-scale transmission measurements.
- the centrifuge of the present invention can be part of a sample processing system, such as known from EP 0 869 838 Bl, which is hereby incorporated by reference.
- sample processing systems that integrate both sample separation systems and sample processing techniques.
- a system can include a sample processing unit configured to perform certain processing steps prior to separation methods, such as magnetic based separation.
- the present invention can include a combined sample processing system and sample separation system.
- Sample processing systems or units can provide sample processing such as cell culturing, washing, preparation, incubation, labeling and the like.
- sample processing systems/units can include centrifugation based separation techniques, where a centrifugal force is applied to a sample so as to separate at least a first component and a second component from a sample.
- a system of the present invention will typically include both a sample processing unit and a sample separation unit.
- the combined processing/separation system of the invention can include a closed system that can programmed to automatically perform a variety of complex cell processing steps including density based separations, immunoaffinity separation, magnetic including immuno-magnetic separations, cell cultivation/ stimulation/ activation, washing or final formulation steps.
- the invention provides a system that minimizes errors of the user, maintains sterility, performs complex cell processing steps with little or no manual interaction, minimizes operator exposure when processing infectious material. Processing at bedside or in surgical room is possible.
- the device can be operated patient connected e. g. bone marrow obtained from a patient may be processed directly into an input bag of the tubing set. From there, the e.g. bone marrow can be processed, i.e. separated into at least two components.
- FIG. 8 An embodiment of such a sample processing system is described with reference to figure 8.
- a processing system including various coupled components, flow channels, buffers, reagents, etc. It will be recognized that numerous configurations are avail- able and that the current configuration is provided for illustrative purposes.
- components include a system buffer 300, spike port 301, sterile filter 302, plasma/in process bag 303, magnetic labeling reagent container 304, spike port 305, magnetic reagent sterile filter 306, sterile filter 307, buffer/media bag 308, cell culture media port, auxiliary port 309, single direction valve downwards 310, single direction valve upwards 311, sample bag 312, sample bag connector 313, sample filter 314, sample port 315, filter 316, pre-separation filter 320, in process storage bag 321, magnetic separation column 322, waste bag 323, volume reduction unit 324, positive fraction bag 325, negative fraction bag 326, sterile air filter 327, pump 328, air filter to pressure sensorl 329, air filter to pressure sensor2 330, sample/cell processing unit 332.
- the centrifugation chamber of the present invention can be used for culturing of cells, similarly to cell culture flasks or bags.
- 3.2E5/ml of the human cell line K562 have been applied to a centrifugation chamber in a volume of 30 ml RPMIl 640 cell culture medium supplemented with 10 % fetal calf serum.
- the chamber was placed in a CO 2 incubator at 5% CO 2 . Aliquots of the content have been removed from the chamber for cell counting and viability assessment after 24, 48, and 70 hours. Seeded cells expanded to 4.1E5/ml, 6.4E5/ml and 9.2E5/ml viable cells at 80 %, 95 % and 95 % viability.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Heart & Thoracic Surgery (AREA)
- Biotechnology (AREA)
- Vascular Medicine (AREA)
- Hematology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Anesthesiology (AREA)
- Cardiology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Developmental Biology & Embryology (AREA)
- Urology & Nephrology (AREA)
- Oncology (AREA)
- Virology (AREA)
Abstract
The invention relates to a centrifuge for separating a sample into at least two components, comprising a chamber for receiving a sample to be centrifuged. According to the invention, the centrifuge further comprises a means for controlling the progress of the sample separation is located at the chamber.
Description
A Centrifuge for Separating a Sample into At Least Two Components
The invention relates to a centrifuge for separating a sample into at least two components and to a method for separating a sample into at least two components in which this centrifuge is used.
Background of the Invention
A centrifugation can be used in a physical method to separate a sample into at least two of its components. In particular, centrifugation is used in the art to separate samples of biological origin into two or more components. These components can then separately be further processed.
When performing a centrifugation, it is advantageous to be able to monitor the progress of the separation of the components that the sample consists of.
Accordingly, the problem underlying the present invention was to provide a means of performing a centrifugation that allowed for monitoring the progress of the separation process.
Brief Summary of the Invention
The problem underlying the present invention is solved both by a centrifuge and a method of using this centrifuge, both as described herein.
In one aspect of the invention, a centrifuge for separating a sample into at least two components is provided. Such a centrifuge comprises a chamber (or processing chamber or rotating container) for receiving a sample to be centrifuged and a means for controlling the progress of the sample separation located at the chamber. The saparation of the sample results in at least a first component and a second component that are separated from each other. The components therefore form layers in the centrifuge chamber that can be detected.
In a preferred embodiment of the invention, the means for controlling the progress of the sample separation is a window, a mirror or a prism that is located such that light from a light source can be transmitted through at least a part of the sample and that the light leaving the prism is detectable by a light detector. Further, the means for controlling the progress of the
sample separation can be a double prism, with two prism sections aligned in a mirror symmetrical fashion.
The chamber of the centrifuge comprises a circular base plate, the center of which is oriented substantially perpendicular to a rotational axis; a cladding or wall which is oriented substantially perpendicular to the base plate such that base plate and cladding together form a pot-like structure (lower portion of the chamber); and a circular cover plate (lid; upper portion of the chamber), which is positionable on the edge of the cladding that is remote from or opposite the base plate and the center of which is oriented substantially perpendicular to the rotational axis. Thereby, a closed centrifugation chamber is formed, consisting of a pot-like bottom part and an upper part in the form of a lid.
In one embodiment, the means for controlling the progress of the sample separation is positioned at the base plate or the cover plate of the chamber. It is preferred that the means for controlling the progress of the sample separation (e.g. the prism or double prism) is positioned at the cover plate of the chamber.
The means for controlling the progress of the sample separation is preferably positioned at a channel or at a gap which is located in the base plate or the cover plate of the chamber such that the sample can enter the channel or gap during the centrifugation such that the sample becomes detectable. In other words, the channel or gap is configured such that at least a part of the sample can flow into it during centrifugation. In particular, the separation of the sample becomes detectable, since light can at least in part penetrate the different components of the sample. Thereby, a signal is generated that allows for determining when the sample separation is complete. In addition, the chamber can comprise an outlet opening that allows for a component of the sample to be drained from the chamber during centrifugation.
Besides the formation of layers, the saparation of the sample can also be detected using the pH value and/or temperature. This will be explained in more detail with reference to the figures.
Preferably, the channel is oriented such that it stretches radially in a linear fashion from an area located at the rotation axis to an area located at a perimeter of the base plate or the cover plate, depending on where the detection means is located. The channel is positioned such that at either side light can pass from the prism or double prism into the channel which holds sample during centrifugation. The detection of the light passing through the sample allows for determining how far the separation of the sample has proceeded and also for determining the position of the borders between different components of the sample. Based on the knowledge of the position of the borders between different components, it becomes possible to drain
certain components from the chamber through at least one outlet port, which is located at the chamber.
In a preferred embodiment, the chamber is configured such that it can serve as or accommodate a container for the cultivation of cells. Thereby, the centrifugation chamber can be used both for cell culture purposes and for processing of the cells grown therein. The chamber allows a large range of cell culture methods to be performed, such as growing of cells, separating, washing, enriching the cells or different kinds of cells, or others. For this purpose, the chamber may comprise further inlet/outlet openings, e.g. for gas, cell culture media or alike. Cell culture conditions are known in the art.
In another aspect of the invention, a method for separating a sample into at least two components is provided. Such a method comprises the steps of providing a sample, and centrifuging the sample in a centrifuge as described above and herein.
As described above and herein, the sample is preferably a biological sample, such as blood, bone marrow, cells, compositions comprising cells or cellular components or alike.
The centrifuge can be part of a sample processing unit. This sample processing unit, may comprise an input port and an output port coupled to centrifuge chamber as described herein having at least one sample chamber, wherein the sample processing unit is configured to provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample. The sample processing unit can be coupled to a sam- pie separation unit to form a system. The sample separation unit can be coupled to the output port of the sample processing unit, wherein the sample separation unit comprises a separation column holder, a pump, and a plurality of valves configured to at least partially control fluid flow through a fluid circuitry and a separation column positioned in the holder, wherein the separation column is configured to separate labeled and unlabeled components of sample flowed through the column.
Brief Description of the Drawings
Figure 1 illustrates a chamber of a centrifuge, according to an embodiment of the present invention.
Figure IA illustrates a chamber of a centrifuge according to an embodiment of the present invention with a light source and a detector for detecting the progress of the sample separation in the chamber.
Figure 2 shows a cross-sectional view of a processing chamber of a centrifuge, according to an embodiment of the present invention.
Figure 2Aillustrates a top plan view of a processing chamber, according to an embodiment of the present invention.
Figure 3 shows a cross-sectional view of a processing chamber, according to another embodiment of the present invention.
Figure 3Aillustrates a focused view of a portion of a processing chamber as shown in Figure
3.
Figure 4 shows a cross-sectional view of a processing chamber, according to yet another embodiment of the present invention.
Figure 4 A shows a view over the bottom of the process chamber with opening for gas delivery and a bonded hydrophobic membrane.
Figure 4B shows an embodiment of the bottom of the chamber with spiral channels for aeration through membrane bonded to the bottom of the chamber.
Figure 5 shows a view of the inside of a lid for a rotating chamber with a channel or gap in which the sample flows during centrifugation, with a means for detecting the progress of separation of the sample in the form of a prism.
Figure 6 shows the path of light through the sample by means of a prism. The prism (double prism) is configured such that light from a light source can at least partially penetrate through at least a part of the sample that is being separated through centrifugation, and light passing through at least a part of the sample can be detected by a light detector.
Figure 7 shows a double prism that is part of a rib located at the lid of the rotating chamber.
Figure 8 illustrates a system with a centrifuge according to an embodiment of the present invention.
DETAILED DESCRIPTION OF THE INVENTION
The present invention provides a centrifuge for separating a sample into at least two components. Such a centrifuge comprises a chamber or processing chamber for receiving a sample to be centrifuged and a means for controlling the progress of the sample separation is located at the chamber.
The sample chamber or chamber, which may also be part of a processing unit (see below), is now further described with reference to Figures 1 through 4.
A processing chamber of a centrifuge according to an embodiment of the present invention is described with reference to Figure 1. The chamber 170 includes an upper portion 172 and a lower portion 174, with a rotational axis 176 and fluid ports or line connect 176, 178 fluidly connected to one or more internal compartments of the chamber 170. The upper portion 172 includes a support structure 182 that is oriented substantially radially, stretching from an area located at the rotation axis 176 to an area located at a perimeter of the upper portion 172, as well as structure 184 including a channel 186 that can include at least a portion that is visible through a window or prism 188. The channel 186 can be fluidly coupled to a sample containment compartment in the chamber 170 and configured for external monitoring or detection of sample processing. For example, a component (e.g., cells) in fluid in the channel 186 may become visibly separated during processing steps, thereby indicating separation of cells or sample components in one or more internal compartments of the chamber. Rotational means or bearing 180 provides rotational movement of the chamber 170 about the axis 176.
The chamber 170 may further comprise at least one vent, preferentially comprising a sterile, hydrophobic membrane or tampon. Preferably, these membranes or tampons may be located at the top or bottom of the chamber. The at least one vent in the chamber has the particular advantage that the volume in the chamber can be changed easily without changing the pressure in the chamber or providing further inlet and/or outlet ports for the exchange of air or gas.
The centrifuges known in the art allow batch-wise centrifugation, i.e. if the volume of the sample to be reduced or concentrated is larger than the chamber, several centrifugation steps are necessary to receive the concentrated product. In one embodiment of the present invention the system allows continuous centrifugation: sample, media, gases and other materials can enter and leave the system e.g. through inlet and outlet ports (e.g. Fig. 1: inlet port 178 and outlet port 180) without stopping the centrifugation process and refilling the centrifuge (batch- wise centrifugation). This allows a continuous concentration of the sample and the product
may be removed only once at the end of the centrifugation, thus avoiding potential contamination due to additional handling.
In figure Ia, a rotating container or centrifugation chamber 500 is shown. At the bottom of the rotating chamber 500 a microscope focus area 505 is positioned which comprises at least one sensor pad 504. Below the rotating chamber 500, a microscope camera module 503 is located that comprises a microscope optics 501 and a microscope drive motor 502 for focusing the optics. The microscope optics 501 is configured such that is can focus automatically to detect the sample that is being separated into at least two components during centrifugation. Thereby, the microscope camera module 503 can be used to detect different layers formed by the separated sample in the chamber 500 due to centrifugal forces. In addition, the pH value of the sample components can be measured. For this purpose, an indicator is used in the chamber 500 that changes its color dependent on the pH value that is present. Moreover, it is possible that the temperature of the sample in the chamber be measured using liquid crystals that are position in the chamber such that their position can be detected with a microscope camera module 503 from the outside. Thereby, the temperature in the chamber 500 can be determined.1
The microscope camera module 503 can be mounted in a movable fashion, such that the mod- ule 503 can be directed with its microscope optics 501 at different sensor pads 504 located in the wall of the chamber 500. This facilitates the detection of various layers formed in the chamber 500 or the detection of the pH or the temperature at different positions within the chamber 500.
Figure 2 illustrates a cross-sectional view of a sample processing chamber according to an embodiment of the present invention. The chamber 190 includes an upper portion 192 and a base portion 194, and one or more internal compartments. The chamber 190 is configured to rotate about an axis so as to apply a centrifugal force to sample disposed in one or more compartments' in the chamber, thereby separating at least two components of the sample. The chamber includes central line 196 fluidly connected to at least one compartment of the chamber. Components of the chamber 190 further include outer line 198; rotational bearing 200, rotational seals 202, 204, 206; outer entry line to the chamber 205; lower radial channel 208; inner line entry 210 to a chamber compartment; slant 212, and deflector 214. Chamber retainer 216 is included and configured for secure positioning/coupling of the chamber 190 with other components of a system of the invention.
The centrifugation chamber 190 preferably comprises a rotating seal, optionally with two fluid lines, preferably with two fluid lines. The fluid lines can enter the chamber 190 at different position. For example, it is possible to position a first fluid line at the outer perimeter of
the upper portion 192 (lid). A second fluid line could be positioned further inward, e.g. 2 mm to 20 mm further towards the center of the chamber 190. Optionally, a vent can be located at the upper portion 192, e.g. in the form of a membrane.
Generally, the position of openings such as holes or line entries in the centrifugation chamber can be configured such that they are best suited for the centrifugation of a particular sample. Depending on the components of a particular sample, and the relative volume of each component in the sample, the openings can be positioned so that the removal and/or detection of a particular component can be achieved.
Figure 2 A illustrates a top plan view of a chamber 201. The chamber 201 includes an inner line 203, lower radial channel 205, inner line entry 207 to the chamber, optionally a deflector 209, slant 211 and a light pass.
Figure 3 illustrates a cross-sectional view of a chamber according to another embodiment of the present invention. The chamber 220 includes an axis about which the chamber rotates, a central line connect 222 and a outer line connect 224, and one or more internal compartments. Further illustrated are rotational bearing 226, as well as rotational seals 228, 230, 232; inner channel 234, optical detection channel 236 (similar to described above); inner line entry 238 to the chamber; inner line 240, and lower radial channel 242. The chamber further includes an inner reinforcement 246 and a chamber retainer 248. Figure 3A illustrates a focused view of portion of a chamber 220 is described above. Shown are an optical detection channel 236, a prism 237, and light pass 239 (further indicated by arrows). It can be seen that light from a light source (not shown) is directed by the prism 237such that is goes through the channel 234 which is filled with sample during centrifugation. The light exiting from the channel 234 is redirected by the prism 237 and can be detected by a detector (not shown).
In another embodiment of the present invention shown in Figure 4A, the bottom of the chamber of the centrifuge can possess one or more openings 291 that may be covered with a hy- drophobic membrane 292. These openings 291 are used for delivery of gases into the chamber, for instance for cell culture processes (such as CO2, N2, O2, etc.). The membrane can be glued, or bonded thermally or by ultrasound or by other means bonded to the bottom of the chamber in a way to assure sterile connection with the chamber. t In another embodiment of the present invention (Fig. 4B), the bottom of the chamber can possess a system of channels for the gas flow, for instance channels assembled as a spiral system 293, which assures a large contact area between the gases and a membrane bonded over the channels (not shown). The channel system possesses at least one input (opening) 294 and an optional output (opening) 295 for the gases.
The entries or ports of the channels of Figs. 1-4A may vary in number and location within the channel. ;
Figure 4 illustrates a cross-sectional view of a chamber according to another embodiment of the present invention. Construction of chamber 250 is similar in many regards to chambers as described above, but further includes a plurality of layered structures 252. The layered structures 252 can be configured to provide cell culture structures or layers. In use, sample including cells can be introduced into the chamber and flowed over layers 252. Separation process- ing can include rotation of the chamber such that cells adhering to the layers are separated from those with lesser affinity for the layers. Intermittent rotation and/or breaking during rotation can further disconnect cultured cells from the surface of the layered structures 252 for separation processing. The chamber further includes an illustrated central line 251 , outer line 253, bearing 255, rotational seals 257, outer line entry 259 to the chamber, upper portion 261, inner channel 263, base portion 265, retainer 267, lower radial channel 269, and inner line entry 271 to the chamber.
The chamber as described herein may comprise or may be made of various materials. In a preferred embodiment, transparent materials are used like plastics, polystyrol (PS), polysterene, polyvinylchloride, polycarbonate, glass, polyacrylate, polyacrylamide, polymethylmethacrylate (PMMA), and/or polyethylenterephtala (PET). Polytetrafluorethylen (PTFE) and /or thermoplastic polyurethane (TPU), silicone or compositions comprising one or more of the above mentioned materials. The chamber can also be made of polyethylene (PE). In a preferred embodiment, the layers in the chamber comprise or are made of collagen, chitin, algi- nate, and/or hyaluronic acid derivatives. Possible are also polyactide (PLA), olyglycolida (PGA) and their copolymers, which are biodegradable. Alternatively, non-biodegradable materials can be used, such as polystyrol (PS), polysterene, polycarbonate, polyacrylate, polyethylene (PE), polymethylmethacrylate (PMMA), and/or polyethylenterephtala (PET). Polytetrafluorethylen (PTFE) and /or thermoplastic polyurethane (TPU) can also be used. Other alternatives include ceramics and glass materials, like hydroxylapatite (HA) or calcium phosphate. The layers in the chamber can be of solid material or porous.
In a preferred embodiment, the chamber has a size of 2 cm to 50 cm in diameter and a height of 5 mm to 50 cm. Centrifugation is preferentially carried out up to 1000 xg. The number of the layers and the distance between the layers is variable. In a preferred embodiment, the chamber can be heated and cooled to provide for a temperature appropriate for the sample to be centrifuged. For this purpose, a heating and/or cooling means can be located at the chamber or surrounding the chamber.
The detection of optical layers in the centrifugal chambers is shown in detail in Figures 5-7.
The cylindrical shaped centrifuge chamber shown in figure 5 is limited on its upper side by a lid 800, which may carry one or more some stabilizing ribs 805 on the flat top surface. At least one of these radial ribs 805 covers a narrow gap or channel 801, open to the centrifuges inner volume when the lid 800 is tied on the centrifuge chamber. The gap 801 extends in axial direction from the inner lid surface passing the lid 800 some millimeters into the rib 805.
Therefore, it may be visible from the outside within the rib 805 when transparent material is used. In radial extension, the gap 801 reaches from near the center up to the cylindrical centri- fuge wall (Fig. 5).
During centrifugation, the same forces take effect in the gap 801 as in the whole centrifuge chamber. The ring shaped neighbored suspension layers extend parallel into the gap 801 and are displayed as axial standing neighbored thin areas, like a thin layers cross cut, well detect- ible by external optical sensors.
The gap 801 width can be determined freely, but need to be small enough for a transmitted light analysis of all layer-associated areas in the gap. Thereby, it is possible to quantify the optical densities and colors of all layers of the suspension in the centrifuge chamber in a "touchless" manner from the outside through optical transmission measurements.
To enable a vertical illumination and sensor position to watch the layers movements in the gap, a prism can be added to a rib, e.g. on both rib sides, which may be preformed by the transparent housing material itself.
The prism 810 refracts the vertical generated illuminating beam through the gap (horizontal) and back to the top, vertical again (Fig. 6). During centrifugation, a synchronous position triggered electronic flash light can transmit light into one side of the prism 810, e.g. the left prism, illuminating the gap by refraction. The transmission result is refracted by the other side of the prism 810, e.g. the right prism, back to a vertical mounted camera, possibly in the neighborhood to the upper flash source on the top. The resulting optical sensor unit is easy to handle like a reflex sensor but at the same time allows for full-scale transmission measurements.
The arrangement of the prism's angles ensures the "total reflection" on its inner prism surface for the illuminating flash beams and avoids direct reflections on its outer surfaces between light source and camera. Therefore, there is no need for mirror coatings and injection molding technologies can be used without rework of the facilities being required (Fig. 7).
In one embodiment, the centrifuge of the present invention can be part of a sample processing system, such as known from EP 0 869 838 Bl, which is hereby incorporated by reference.
Such sample processing systems that integrate both sample separation systems and sample processing techniques. A system can include a sample processing unit configured to perform certain processing steps prior to separation methods, such as magnetic based separation. As such, the present invention can include a combined sample processing system and sample separation system. Sample processing systems or units can provide sample processing such as cell culturing, washing, preparation, incubation, labeling and the like. Additionally, sample processing systems/units can include centrifugation based separation techniques, where a centrifugal force is applied to a sample so as to separate at least a first component and a second component from a sample.
Thus, a system of the present invention will typically include both a sample processing unit and a sample separation unit. The combined processing/separation system of the invention can include a closed system that can programmed to automatically perform a variety of complex cell processing steps including density based separations, immunoaffinity separation, magnetic including immuno-magnetic separations, cell cultivation/ stimulation/ activation, washing or final formulation steps. The invention provides a system that minimizes errors of the user, maintains sterility, performs complex cell processing steps with little or no manual interaction, minimizes operator exposure when processing infectious material. Processing at bedside or in surgical room is possible. The device can be operated patient connected e. g. bone marrow obtained from a patient may be processed directly into an input bag of the tubing set. From there, the e.g. bone marrow can be processed, i.e. separated into at least two components.
Accordingly, an embodiment of such a sample processing system is described with reference to figure 8. As illustrated, a processing system including various coupled components, flow channels, buffers, reagents, etc. It will be recognized that numerous configurations are avail- able and that the current configuration is provided for illustrative purposes. Referring to figure 8, components include a system buffer 300, spike port 301, sterile filter 302, plasma/in process bag 303, magnetic labeling reagent container 304, spike port 305, magnetic reagent sterile filter 306, sterile filter 307, buffer/media bag 308, cell culture media port, auxiliary port 309, single direction valve downwards 310, single direction valve upwards 311, sample bag 312, sample bag connector 313, sample filter 314, sample port 315, filter 316, pre-separation filter 320, in process storage bag 321, magnetic separation column 322, waste bag 323, volume reduction unit 324, positive fraction bag 325, negative fraction bag 326, sterile air filter 327, pump 328, air filter to pressure sensorl 329, air filter to pressure sensor2 330, sample/cell processing unit 332.
Example: Cell Culture
The centrifugation chamber of the present invention can be used for culturing of cells, similarly to cell culture flasks or bags.
3.2E5/ml of the human cell line K562 have been applied to a centrifugation chamber in a volume of 30 ml RPMIl 640 cell culture medium supplemented with 10 % fetal calf serum. The chamber was placed in a CO2 incubator at 5% CO2. Aliquots of the content have been removed from the chamber for cell counting and viability assessment after 24, 48, and 70 hours. Seeded cells expanded to 4.1E5/ml, 6.4E5/ml and 9.2E5/ml viable cells at 80 %, 95 % and 95 % viability.
Claims
1. A centrifuge for separating a sample into at least two components, comprising: a chamber for receiving a sample to be centrifuged, characterized in that a means for controlling the progress of the sample separation is located at the chamber.
2. The centrifuge according to claim 1, characterized in that the chamber comprises a circular base plate, the center of which is oriented substantially perpendicular to a rotational axis, a cladding which is oriented substantially perpendicular to the base plate, and - a circular cover plate, which is positionable on the edge of the cladding that is remote from the base plate.
3. The centrifuge according to claim 1 or 2, characterized in that the means for controlling the progress of the sample separation is positioned at the base plate or the cover plate.
4. The centrifuge according to claims 1 to 3, characterized in that the means for detecting the progress of separation is located such that
- light from a light source can at least partially penetrate through at least a part of the sample that is being separated, and
- light passing through at least a part of the sample can be detected by a light detector.
5. The centrifuge according to claims 1 to 4, characterized in that the means for controlling the progress of the sample separation is positioned at a channel or gap located in the base plate or the cover plate, into which the sample can enter during centrifugation such that it becomes detectable.
6. The centrifuge according to claim 1 to 5, characterized in that the means for detecting the progress of separation is located at the rotating container essentially perpendicular to a rotation axis of the rotating container.
5. The centrifuge according to claim 4, characterized in that the channel or gap is oriented such that it extends radially from an area located at the rotation axis of the base plate or the cover plate to an area located at a perimeter of the base plate or the cover plate.
6. The centrifuge according to claim 1 to 5, characterized in that the means for controlling the progress of the sample separation is a window, a mirror, or a prism that is located such that light from a light source can be transmitted through at least a part of the sample and that the light leaving the window, mirror, or prism, respectively, is detectable by a light detector.
7. The system according to claims 1 to 6, characterized in that the window, prism, or mirror is positioned to cover the channel or gap.
8. The centrifuge according to claims 1 to 7, characterized in that the chamber is configured such that it can serve as a container for the cultivation of cells.
9. The system according to claims 1 to 8, characterized in that the chamber comprises at least one layer for growing cells thereon.
10. The system according to claims 1 to 9, characterized in that the chamber is disposable and/or can be sterilized.
11. The system according to claims 1 to 10, characterized in that the chamber comprises or can be made of a material chosen from the group consisting of: plastics, polystyrol (PS), polysterene, polyvinylchloride, polycarbonate, glass, polyacrylate, polyacryla- mide, polymethylmethacrylate (PMMA), polyethylenterephtala (PET), polytetrafluore- thylen (PTFE), thermoplastic polyurethane (TPU), silicone The chamber can also be made of polyethylene (PE), collagen, chitin, alginate, hyaluronic acid derivatives, polyactide (PLA), olyglycolida (PGA) and their copolymers, polystyrol (PS), polysterene, polycarbonate, polyacrylate, ceramics, glass materials, like hydorxylapatite (HA), and calcium phosphate, and compositions comprising one or more of the above mentioned materials.
12. A method for separating a sample into at least two components, comprising
- providing a sample that can be separated into at least two components, and
- centrifuging the sample in a centrifuge according to claims 1 to 11.
13. The method according to claim 12, further comprising: detecting the progress of separation, in particular by detecting the formation of layers of the sample, the change of pH value, and/or the change of temperature.
14. The method according to claim 12 or 13, whereby the sample is a biological sample, such as blood, bone marrow, cells, a composition comprising cells and/or cellular components, or alike.
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES08858277T ES2374863T3 (en) | 2007-12-07 | 2008-12-08 | CENTRIFUGAL TO SEPARATE A SAMPLE IN AT LEAST TWO COMPONENTS. |
EP08858277A EP2227334B1 (en) | 2007-12-07 | 2008-12-08 | A centrifuge for separating a sample into at least two components |
AT08858277T ATE528077T1 (en) | 2007-12-07 | 2008-12-08 | CENTRIFUGE FOR SEPARATING A SAMPLE INTO AT LEAST TWO COMPONENTS |
US12/745,689 US8747290B2 (en) | 2007-12-07 | 2008-12-08 | Centrifuge for separating a sample into at least two components |
JP2010536549A JP5456689B2 (en) | 2007-12-07 | 2008-12-08 | A centrifuge that separates a sample into at least two components |
US14/201,744 US9714945B2 (en) | 2007-12-07 | 2014-03-07 | Centrifuge for separating a sample into at least two components |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US1236107P | 2007-12-07 | 2007-12-07 | |
US61/012,361 | 2007-12-07 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/745,689 A-371-Of-International US8747290B2 (en) | 2007-12-07 | 2008-12-08 | Centrifuge for separating a sample into at least two components |
US14/201,744 Division US9714945B2 (en) | 2007-12-07 | 2014-03-07 | Centrifuge for separating a sample into at least two components |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009072006A2 true WO2009072006A2 (en) | 2009-06-11 |
WO2009072006A3 WO2009072006A3 (en) | 2009-08-06 |
Family
ID=40637746
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2008/003845 WO2009072006A2 (en) | 2007-12-07 | 2008-12-08 | A centrifuge for separating a sample into at least two components |
PCT/IB2008/003823 WO2009072003A2 (en) | 2007-12-07 | 2008-12-08 | Sample processing system and methods |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2008/003823 WO2009072003A2 (en) | 2007-12-07 | 2008-12-08 | Sample processing system and methods |
Country Status (9)
Country | Link |
---|---|
US (13) | US8747290B2 (en) |
EP (6) | EP3338895B1 (en) |
JP (5) | JP5456689B2 (en) |
AT (1) | ATE528077T1 (en) |
DK (3) | DK2227271T3 (en) |
ES (5) | ES2660180T3 (en) |
HU (1) | HUE034888T2 (en) |
NO (1) | NO2227271T3 (en) |
WO (2) | WO2009072006A2 (en) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2664383A1 (en) | 2012-05-15 | 2013-11-20 | Miltenyi Biotec GmbH | Centrifugation chamber with deflector shields |
EP2821145A1 (en) | 2013-07-02 | 2015-01-07 | Miltenyi Biotec GmbH | Centrifugation chamber with gas-permeable membranes layers for cell cultivation |
EP2821144A1 (en) | 2013-07-02 | 2015-01-07 | Miltenyi Biotec GmbH | Centrifugation chamber with gas-permeable membrane for cell cultivation |
US9682185B2 (en) | 2010-11-23 | 2017-06-20 | Haemonetics Corporation | Apheresis bowl with improved vibration characteristics |
US10006840B2 (en) | 2011-11-25 | 2018-06-26 | Miltenyi Biotec Gmbh | Technology for purifying NK cells and other cell types by concurrent gravity sedimentation and magnetic separation |
EP3342855A1 (en) | 2016-12-27 | 2018-07-04 | Miltenyi Biotec GmbH | Cell composition depleted from tcrab and cd45ra positive cells |
US10053663B2 (en) | 2013-07-02 | 2018-08-21 | Miltenyi Biotec, Gmbh | Centrifugation chamber with gas permeable membrane layers for cell cultivation |
CN108680430A (en) * | 2018-03-15 | 2018-10-19 | 水利部交通运输部国家能源局南京水利科学研究院 | A kind of tail residues centrifugal model seepage flow with consolidation control system and test method |
CN109843317A (en) * | 2016-08-29 | 2019-06-04 | 哈肯萨克大学医学中心 | The composition and method for by the stemness rich in blood platelet fraction of the blood containing platelets-like cell in people being used to that adult cell to be made to reprogram |
CN113046321A (en) * | 2014-04-23 | 2021-06-29 | 朱诺治疗学股份有限公司 | Methods of isolating, culturing and genetically engineering immune cell populations for adoptive therapy |
US12066365B2 (en) | 2012-02-23 | 2024-08-20 | Juno Therapeutics Gmbh | Chromatographic isolation of cells and other complex biological materials |
Families Citing this family (284)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ITBO20050481A1 (en) | 2005-07-19 | 2007-01-20 | Silicon Biosystems S R L | METHOD AND APPARATUS FOR THE HANDLING AND / OR IDENTIFICATION OF PARTICLES |
ITBO20050646A1 (en) | 2005-10-26 | 2007-04-27 | Silicon Biosystem S R L | METHOD AND APPARATUS FOR CHARACTERIZATION AND COUNTING OF PARTICLES |
ITTO20060226A1 (en) | 2006-03-27 | 2007-09-28 | Silicon Biosystem S P A | METHOD AND APPARATUS FOR PROCESSING AND OR ANALYSIS AND OR SELECTION OF PARTICLES, IN PARTICULAR BIOLOGICAL PARTICLES |
ITTO20070771A1 (en) | 2007-10-29 | 2009-04-30 | Silicon Biosystems Spa | METHOD AND APPARATUS FOR IDENTIFICATION AND HANDLING OF PARTICLES |
JP5456689B2 (en) | 2007-12-07 | 2014-04-02 | ミルテンイ バイオテック ゲーエムベーハー | A centrifuge that separates a sample into at least two components |
IT1391619B1 (en) | 2008-11-04 | 2012-01-11 | Silicon Biosystems Spa | METHOD FOR THE IDENTIFICATION, SELECTION AND ANALYSIS OF TUMOR CELLS |
US10895575B2 (en) | 2008-11-04 | 2021-01-19 | Menarini Silicon Biosystems S.P.A. | Method for identification, selection and analysis of tumour cells |
JP5834001B2 (en) * | 2009-03-17 | 2015-12-16 | シリコン・バイオシステムズ・ソシエタ・ペル・アチオニ | Microfluidic device for separating cells |
US9476812B2 (en) | 2010-04-21 | 2016-10-25 | Dna Electronics, Inc. | Methods for isolating a target analyte from a heterogeneous sample |
US20110262989A1 (en) * | 2010-04-21 | 2011-10-27 | Nanomr, Inc. | Isolating a target analyte from a body fluid |
US8852532B2 (en) * | 2010-06-18 | 2014-10-07 | Roche Diagnostics Operations, Inc. | G-force sensitive label and corresponding sample tube, method and analytical system |
US8870733B2 (en) * | 2010-11-19 | 2014-10-28 | Kensey Nash Corporation | Centrifuge |
AU2011343735A1 (en) | 2010-12-15 | 2013-07-18 | Cytosed, Inc. | Antibody-linked immuno-sedimentation agent and method of isolating a target from a sample using same |
US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
EP2739587B1 (en) | 2011-08-01 | 2020-05-27 | Denovo Sciences | Cell capture system |
US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
DE102011109332A1 (en) | 2011-08-03 | 2013-02-07 | Eppendorf Ag | Laboratory apparatus and method for treating laboratory samples |
ITTO20110990A1 (en) | 2011-10-28 | 2013-04-29 | Silicon Biosystems Spa | METHOD AND APPARATUS FOR OPTICAL ANALYSIS OF LOW TEMPERATURE PARTICLES |
US9075039B2 (en) | 2011-11-08 | 2015-07-07 | Becton, Dickinson And Company | Container and cap for a biological specimen |
EP2594632A1 (en) | 2011-11-18 | 2013-05-22 | Miltenyi Biotec GmbH | Method and device for cell modification |
EP2795289B1 (en) * | 2011-12-21 | 2022-08-31 | Becton Dickinson and Company | Flow cytometric systems for sterile separation of magnetically labeled sample components |
ITBO20110766A1 (en) | 2011-12-28 | 2013-06-29 | Silicon Biosystems Spa | DEVICES, EQUIPMENT, KITS AND METHOD FOR THE TREATMENT OF A BIOLOGICAL SAMPLE |
CH706420A1 (en) * | 2012-04-19 | 2013-10-31 | Valerie Soulie | Method for the viral inactivation of a biological fluid, device and container for carrying & oelig; piece of such a method. |
WO2013168767A1 (en) * | 2012-05-10 | 2013-11-14 | コニカミノルタ株式会社 | Process for removing red blood cells and centrifugal tube for blood collection |
EP2711418B1 (en) * | 2012-09-25 | 2017-08-23 | Miltenyi Biotec GmbH | Method for polyclonal stimulation of T cells by flexible nanomatrices |
US9265925B2 (en) * | 2012-10-16 | 2016-02-23 | Acist Medical Systems, Inc. | Controlling flow in a medical injection system |
US9551704B2 (en) | 2012-12-19 | 2017-01-24 | Dna Electronics, Inc. | Target detection |
US9434940B2 (en) | 2012-12-19 | 2016-09-06 | Dna Electronics, Inc. | Methods for universal target capture |
US9599610B2 (en) | 2012-12-19 | 2017-03-21 | Dnae Group Holdings Limited | Target capture system |
US9995742B2 (en) | 2012-12-19 | 2018-06-12 | Dnae Group Holdings Limited | Sample entry |
US10000557B2 (en) | 2012-12-19 | 2018-06-19 | Dnae Group Holdings Limited | Methods for raising antibodies |
US9606102B2 (en) * | 2013-01-26 | 2017-03-28 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
GB2525562B (en) * | 2013-02-15 | 2017-10-11 | T Gjerde Douglas | Methods for purification of biological cells |
US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
US9856535B2 (en) | 2013-05-31 | 2018-01-02 | Denovo Sciences, Inc. | System for isolating cells |
CN105339090B (en) * | 2013-06-28 | 2017-03-08 | 独立行政法人产业技术综合研究所 | Magnetic separator medium and magnetic separator |
CN103333799A (en) * | 2013-07-09 | 2013-10-02 | 广州一代医药科技有限公司 | Magnetic cell sorting apparatus based on nanoferritin adsorption |
ES2612918T3 (en) | 2013-07-10 | 2017-05-19 | Miltenyi Biotec Gmbh | Method to induce the proliferation of natural killer cells by mobile nanomatrices |
WO2015160320A1 (en) * | 2014-04-15 | 2015-10-22 | Promedart Biyoteknoloji Ve Ozel Saglik Hizmetleri Sanayi Ticaret Limited Sirketi | A separation system |
PL3132247T3 (en) | 2014-04-16 | 2022-01-03 | Juno Therapeutics Gmbh | Methods, kits and apparatus for expanding a population of cells |
US9493789B2 (en) * | 2014-04-24 | 2016-11-15 | Miltenyi Biotec Gmbh | Automated generation of genetically modified stem cells |
US10131876B2 (en) * | 2014-04-24 | 2018-11-20 | Miltenyi Biotec Gmbh | Method for automated generation of genetically modified T cells |
SG11201610017SA (en) | 2014-05-30 | 2016-12-29 | Finesse Solutions Inc | Aseptic connectors for bio-processing containers |
US9346578B2 (en) * | 2014-05-30 | 2016-05-24 | Finesse Solutions, Inc. | Aseptic connectors for bio-processing containers |
KR20170032406A (en) | 2014-07-15 | 2017-03-22 | 주노 쎄러퓨티크스 인코퍼레이티드 | Engineered cells for adoptive cell therapy |
ES2767399T3 (en) | 2014-08-19 | 2020-06-17 | Miltenyi Biotec Bv & Co Kg | Chimeric antigen receptor specific for the SSEA4 antigen |
TWI751102B (en) | 2014-08-28 | 2022-01-01 | 美商奇諾治療有限公司 | Antibodies and chimeric antigen receptors specific for cd19 |
CN107106610A (en) | 2014-10-20 | 2017-08-29 | 朱诺治疗学股份有限公司 | The composition and method of administration in being treated for adoptive cellular |
KR20240023204A (en) * | 2014-11-05 | 2024-02-20 | 주노 쎄러퓨티크스 인코퍼레이티드 | Methods for transduction and cell processing |
ES2819553T3 (en) | 2014-12-03 | 2021-04-16 | Juno Therapeutics Inc | Methods and compositions for adoptive cell therapy |
US10421081B2 (en) | 2014-12-19 | 2019-09-24 | National Technology & Engineering Solutions Of Sandia, Llc | Centrifuge with vector-seeking linkage |
EP3533876B1 (en) | 2014-12-19 | 2024-05-22 | Biosafe S.A. | Sequential processing of biological fluids |
US10655097B2 (en) | 2014-12-22 | 2020-05-19 | Saint-Gobain Performance Plastics Corporation | T-cell culture double bag assembly |
US10280390B2 (en) | 2014-12-22 | 2019-05-07 | Saint-Gobain Performance Plastics Corporation | System for culture of cells in a controlled environment |
US9926524B2 (en) | 2014-12-22 | 2018-03-27 | Saint-Gobain Performance Plastics Corporation | Gas permeable material |
MA41346A (en) | 2015-01-12 | 2017-11-21 | Juno Therapeutics Inc | POST-TRANSCRIPTIONAL REGULATORY ELEMENTS OF MODIFIED HEPATITIS |
EP3245231B1 (en) | 2015-01-16 | 2020-08-12 | Juno Therapeutics, Inc. | Antibodies and chimeric antigen receptors specific for ror1 |
JP6734283B2 (en) * | 2015-01-21 | 2020-08-05 | フレッド ハッチンソン キャンサー リサーチ センター | Point of care and/or portable platform for gene therapy |
EP3061809B1 (en) | 2015-02-27 | 2019-01-30 | Miltenyi Biotec GmbH | Method for generation of a cell composition of mesencephalic dopaminergic progenitor cells |
WO2016166568A1 (en) | 2015-04-16 | 2016-10-20 | Juno Therapeutics Gmbh | Methods, kits and apparatus for expanding a population of cells |
EP3091032A1 (en) | 2015-05-08 | 2016-11-09 | Miltenyi Biotec GmbH | Humanized antibody or fragment thereof specific for cd3 |
EP3998335A3 (en) | 2015-05-08 | 2022-07-27 | Biomagnetic Solutions LLC | Apparatus and method for immunomagnetic cell separation |
JP6457338B2 (en) * | 2015-05-28 | 2019-01-23 | 株式会社日立製作所 | Liquid reflux container, cell concentrator and cell concentrator system |
JP6949728B2 (en) | 2015-05-29 | 2021-10-13 | ジュノー セラピューティクス インコーポレイテッド | Compositions and Methods for Modulating Inhibitory Interactions in Genetically Engineered Cells |
TWI821751B (en) * | 2015-06-05 | 2023-11-11 | 瑞士商諾華公司 | Flow-through magnetic separation/debeading module and method of paramagnetic particle-bound cell processing |
GB201511129D0 (en) * | 2015-06-24 | 2015-08-05 | Linea Ab Q | Method of determining antimicrobial susceptibility of a microorganism |
MA42895A (en) | 2015-07-15 | 2018-05-23 | Juno Therapeutics Inc | MODIFIED CELLS FOR ADOPTIVE CELL THERAPY |
EP3138905A1 (en) | 2015-09-04 | 2017-03-08 | Miltenyi Biotec GmbH | Method for natural killer cell expansion |
EP3138920B1 (en) | 2015-09-07 | 2018-06-13 | Miltenyi Biotec GmbH | Disposable cartridge for electroporation |
US10077422B2 (en) * | 2015-09-28 | 2018-09-18 | The University Of Toledo | Microalgae harvesting using stimuli-sensitive hydrogels |
EP3365453A2 (en) | 2015-10-22 | 2018-08-29 | Juno Therapeutics GmbH | Methods, kits, agents and apparatuses for transduction |
MA45489A (en) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | CELL CULTURE PROCESSES, ASSOCIATED KITS AND APPARATUS |
MA45488A (en) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | CELL CULTURE PROCESSES, KITS AND APPARATUS |
EP3371295A1 (en) * | 2015-11-04 | 2018-09-12 | Northeastern University | Systems for producing cellular immunotherapeutics and methods of use thereof |
US11020429B2 (en) | 2015-11-05 | 2021-06-01 | Juno Therapeutics, Inc. | Vectors and genetically engineered immune cells expressing metabolic pathway modulators and uses in adoptive cell therapy |
MA44314A (en) | 2015-11-05 | 2018-09-12 | Juno Therapeutics Inc | CHEMERICAL RECEPTORS CONTAINING TRAF-INDUCING DOMAINS, AND ASSOCIATED COMPOSITIONS AND METHODS |
AU2016363025B2 (en) | 2015-12-03 | 2021-04-08 | Juno Therapeutics, Inc. | Modified chimeric receptors and related compositions and methods |
EP3383419B1 (en) | 2015-12-03 | 2022-08-03 | Juno Therapeutics, Inc. | Compositions and methods for reducing immune responses against chimeric antigen receptors |
EP3384294B1 (en) | 2015-12-04 | 2021-10-13 | Juno Therapeutics, Inc. | Methods and compositions related to toxicity associated with cell therapy |
RU2729116C2 (en) | 2015-12-16 | 2020-08-04 | Гритстоун Онколоджи, Инк. | Identification, production and use of neoantigens |
EP3184548A1 (en) | 2015-12-23 | 2017-06-28 | Miltenyi Biotec GmbH | Chimeric antigen receptor with cytokine receptor activating or blocking domain |
JP2019509763A (en) * | 2016-03-07 | 2019-04-11 | ヒタチ ケミカル アドヴァンスド セラピューティクス ソリューションズ リミテッド ライアビリティ カンパニー | Closed system for labeling and selecting live cells |
EP3430549A1 (en) | 2016-03-16 | 2019-01-23 | Juno Therapeutics, Inc. | Methods for adaptive design of a treatment regimen and related treatments |
US20190287013A1 (en) | 2016-03-16 | 2019-09-19 | Juno Therapeutics, Inc. | Methods for determining dosing of a therapeutic agent and related treatments |
SG11201808014SA (en) | 2016-03-18 | 2018-10-30 | Qt Holdings Corp | Compositions, devices, and methods for cell separation |
EP3433276B1 (en) | 2016-03-22 | 2021-12-22 | Seattle Children's Hospital (DBA Seattle Children's Research Institute) | Early intervention methods to prevent or ameliorate toxicity |
AU2017249049A1 (en) | 2016-04-15 | 2018-10-25 | Becton, Dickinson And Company | Enclosed droplet sorter and methods of using the same |
ES2885833T3 (en) | 2016-04-20 | 2021-12-15 | Centro De Investig Energeticas Medioambientales Y Tecnologicas O A M P | Composition and methods to enhance PKLR gene expression |
EP3448564B1 (en) | 2016-04-29 | 2021-06-09 | Creoptix AG | Methods and assemblies for molecule recovery |
US10449283B2 (en) | 2016-04-29 | 2019-10-22 | Fenwal, Inc. | System and method for selecting and culturing cells |
US10251990B2 (en) | 2016-04-29 | 2019-04-09 | Fenwal, Inc. | System and method for processing, incubating, and/or selecting biological cells |
EP3241905A1 (en) | 2016-05-06 | 2017-11-08 | Miltenyi Biotec GmbH | Method for introducing nucleic acids into a cell |
CA3026318C (en) | 2016-06-01 | 2023-04-04 | Miltenyi Biotec Gmbh | Process for generation, identification and isolation of human pluripotent stem cell-derived cardiomyocytes and cardiomyocyte subpopulations |
AU2017274733A1 (en) | 2016-06-03 | 2018-12-20 | Memorial Sloan-Kettering Cancer Center | Adoptive cell therapies as early treatment options |
MA45341A (en) | 2016-06-06 | 2019-04-10 | Hutchinson Fred Cancer Res | METHODS FOR TREATING B-LYMPHOCYTE MALIGNITIES USING ADOPTIVE CELL THERAPY |
US20200182884A1 (en) | 2016-06-27 | 2020-06-11 | Juno Therapeutics, Inc. | Method of identifying peptide epitopes, molecules that bind such epitopes and related uses |
MA45491A (en) | 2016-06-27 | 2019-05-01 | Juno Therapeutics Inc | CMH-E RESTRICTED EPITOPES, BINDING MOLECULES AND RELATED METHODS AND USES |
AU2017299804B2 (en) * | 2016-07-22 | 2019-10-31 | Alfa Wassermann, Inc. | Fluid handling systems and method for ultracentrifuges |
WO2018023094A1 (en) | 2016-07-29 | 2018-02-01 | Juno Therapeutics, Inc. | Methods for assessing the presence or absence of replication competent virus |
EP3490585B1 (en) | 2016-07-29 | 2023-05-17 | Juno Therapeutics, Inc. | Immunomodulatory polypeptides and related compositions and methods |
MX2019001184A (en) | 2016-07-29 | 2019-09-26 | Juno Therapeutics Inc | Anti-idiotypic antibodies against anti-cd19 antibodies. |
DE102016114043B3 (en) | 2016-07-29 | 2017-08-10 | Technische Universität Dresden | Device for isolating stem cells from fetal tissues |
CN109863399B (en) | 2016-08-26 | 2022-12-20 | 朱诺治疗学股份有限公司 | Method for counting particles present in a cell composition |
RU2755725C2 (en) | 2016-09-12 | 2021-09-20 | Джуно Терапьютикс, Инк. | Assembly units of perfusion bioreactor bags |
CA3037086A1 (en) | 2016-10-03 | 2018-04-12 | Juno Therapeutics, Inc. | Hpv-specific binding molecules |
US12024702B2 (en) * | 2016-10-10 | 2024-07-02 | Biomerieux | Method and device for preparing samples |
CA3037387A1 (en) | 2016-10-13 | 2018-04-19 | Juno Therapeutics, Inc. | Immunotherapy methods and compositions involving tryptophan metabolic pathway modulators |
US10617720B2 (en) | 2016-10-20 | 2020-04-14 | Miltenyi Biotech, GmbH | Chimeric antigen receptor specific for tumor cells |
EP3315511A1 (en) | 2016-10-29 | 2018-05-02 | Miltenyi Biotec GmbH | Adapter chimeric antigen receptor expressing cells for targeting of multiple antigens |
CN110139669A (en) | 2016-11-03 | 2019-08-16 | 朱诺治疗学股份有限公司 | The combination treatment of T cell therapy and BTK inhibitor |
CA3040914A1 (en) | 2016-11-03 | 2018-05-24 | Juno Therapeutics, Inc. | Combination therapy of a cell based therapy and a microglia inhibitor |
MY192618A (en) * | 2016-11-23 | 2022-08-29 | Illumina Inc | Flow cell assembly securement system and method |
US10718786B2 (en) | 2016-11-23 | 2020-07-21 | Illumina, Inc. | Flow cell assembly securement system and method |
CA3045323A1 (en) | 2016-12-02 | 2018-06-07 | Juno Therapeutics, Inc. | Engineered b cells and related compositions and methods |
KR20190104528A (en) | 2016-12-03 | 2019-09-10 | 주노 쎄러퓨티크스 인코퍼레이티드 | How to Determine CAR-T Cells Administration |
MX2019006288A (en) | 2016-12-03 | 2020-10-01 | Juno Therapeutics Inc | Methods and compositions for use of therapeutic t cells in combination with kinase inhibitors. |
EP3548083A1 (en) | 2016-12-03 | 2019-10-09 | Juno Therapeutics, Inc. | Methods for modulation of car-t cells |
US20190350978A1 (en) | 2016-12-05 | 2019-11-21 | Juno Therapeutics, Inc. | Production of engineered cells for adoptive cell therapy |
EP3336107A1 (en) | 2016-12-15 | 2018-06-20 | Miltenyi Biotec GmbH | Immune cells expressing an antigen binding receptor and a chimeric costimulatory receptor |
US10274495B2 (en) | 2016-12-21 | 2019-04-30 | Fenwal, Inc. | System and method for separating cells incorporating magnetic separation |
CN110382014A (en) * | 2017-01-08 | 2019-10-25 | 赛斯卡医疗有限公司 | Device and method for biological processing cell sample |
CN110691792A (en) | 2017-01-10 | 2020-01-14 | 朱诺治疗学股份有限公司 | Epigenetic analysis of cell therapies and related methods |
EP3571221A2 (en) | 2017-01-20 | 2019-11-27 | Juno Therapeutics GmbH | Cell surface conjugates and related cell compositions and methods |
KR20240063204A (en) | 2017-02-17 | 2024-05-10 | 프레드 허친슨 캔서 센터 | Combination therapies for treatment of bcma-related cancers and autoimmune disorders |
WO2018157171A2 (en) | 2017-02-27 | 2018-08-30 | Juno Therapeutics, Inc. | Compositions, articles of manufacture and methods related to dosing in cell therapy |
JP7359751B2 (en) | 2017-03-14 | 2023-10-11 | ジュノー セラピューティクス インコーポレイテッド | Methods for cryogenic storage |
KR102609624B1 (en) | 2017-03-15 | 2023-12-05 | 옥스포드 바이오메디카(유케이) 리미티드 | method |
MX2019012017A (en) | 2017-04-07 | 2020-02-12 | Juno Therapeutics Inc | Engineered cells expressing prostate-specific membrane antigen (psma) or a modified form thereof and related methods. |
AU2018251188A1 (en) | 2017-04-14 | 2019-10-31 | Juno Therapeutics, Inc. | Methods for assessing cell surface glycosylation |
CN111032850B (en) | 2017-04-27 | 2024-08-13 | 朱诺治疗学有限公司 | Oligomeric particle reagents and methods of use thereof |
FI3618842T3 (en) | 2017-05-01 | 2023-12-15 | Juno Therapeutics Inc | Combination of a cell therapy and an immunomodulatory compound |
WO2018223101A1 (en) | 2017-06-02 | 2018-12-06 | Juno Therapeutics, Inc. | Articles of manufacture and methods for treatment using adoptive cell therapy |
CN111201438A (en) | 2017-06-02 | 2020-05-26 | 朱诺治疗学股份有限公司 | Articles and methods relating to toxicity associated with cell therapy |
US20210147807A1 (en) | 2017-06-14 | 2021-05-20 | Helmholtz Zentrum Munchen - Deutsches Forschungszentrum Fur Gesundheit Und Umwelt (Gmbh) | Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells |
CN111050545A (en) | 2017-06-29 | 2020-04-21 | 朱诺治疗学股份有限公司 | Mouse model for evaluating toxicity associated with immunotherapy |
JP7337773B2 (en) | 2017-07-29 | 2023-09-04 | ジュノー セラピューティクス インコーポレイテッド | Reagents for expanding cells expressing recombinant receptors |
MA49981A (en) | 2017-08-09 | 2020-06-17 | Juno Therapeutics Inc | PROCESSES AND COMPOSITIONS FOR THE PREPARATION OF GENETICALLY MODIFIED CELLS |
AU2018313950A1 (en) | 2017-08-09 | 2020-02-13 | Juno Therapeutics, Inc. | Methods for producing genetically engineered cell compositions and related compositions |
US10391493B2 (en) | 2017-08-29 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
JP2020528284A (en) | 2017-09-01 | 2020-09-24 | ロンザ ウォーカーズヴィル,インコーポレーテッド | Automation of end-to-end cell therapy |
AU2018323875A1 (en) | 2017-09-01 | 2020-04-23 | Gpb Scientific, Inc. | Methods for preparing therapeutically active cells using microfluidics |
EP3676403A1 (en) | 2017-09-01 | 2020-07-08 | Juno Therapeutics, Inc. | Gene expression and assessment of risk of developing toxicity following cell therapy |
MA50079A (en) | 2017-09-07 | 2020-07-15 | Juno Therapeutics Inc | METHODS FOR IDENTIFYING CELLULAR CHARACTERISTICS RELATING TO RESPONSES ASSOCIATED WITH CELL THERAPY |
KR102677029B1 (en) * | 2017-09-21 | 2024-06-24 | 리플리겐 코포레이션 | Method and system for removing pressure and air from a chromatography column |
CA3080546A1 (en) | 2017-10-03 | 2019-04-11 | Juno Therapeutics, Inc. | Hpv-specific binding molecules |
KR20200087143A (en) | 2017-10-10 | 2020-07-20 | 그릿스톤 온콜로지, 인코포레이티드 | Identification of new antigens using hot spots |
JP7403461B2 (en) | 2017-10-16 | 2023-12-22 | セントロ デ インベスティガシオンス エネルジェチカス メディオアンビエンタゥス イェ テクノロジカス オー.エイ. エム.ピー. | Lentiviral vectors for PKLR delivery to treat pyruvate kinase deficiency |
JP7258899B2 (en) | 2017-11-01 | 2023-04-17 | ジュノー セラピューティクス インコーポレイテッド | Methods for making T cell compositions |
US12031975B2 (en) | 2017-11-01 | 2024-07-09 | Juno Therapeutics, Inc. | Methods of assessing or monitoring a response to a cell therapy |
CN111542596A (en) | 2017-11-01 | 2020-08-14 | 朱诺治疗学股份有限公司 | Methods of producing therapeutic compositions of engineered cells |
US11564946B2 (en) | 2017-11-01 | 2023-01-31 | Juno Therapeutics, Inc. | Methods associated with tumor burden for assessing response to a cell therapy |
SG11202003501XA (en) | 2017-11-01 | 2020-05-28 | Juno Therapeutics Inc | Antibodies and chimeric antigen receptors specific for b-cell maturation antigen |
CN111902159A (en) | 2017-11-01 | 2020-11-06 | 朱诺治疗学股份有限公司 | Chimeric antigen receptor specific for B Cell Maturation Antigen (BCMA) |
US20200289565A1 (en) | 2017-11-06 | 2020-09-17 | Juno Therapeutics, Inc. | Combination of a cell therapy and a gamma secretase inhibitor |
KR20200095487A (en) | 2017-11-10 | 2020-08-10 | 주노 쎄러퓨티크스 인코퍼레이티드 | Closed-system cryogenic vessel |
US11885815B2 (en) | 2017-11-22 | 2024-01-30 | Gritstone Bio, Inc. | Reducing junction epitope presentation for neoantigens |
JP7170042B2 (en) * | 2017-11-30 | 2022-11-11 | ヤンタイ・アウスビオ・ラボラトリーズ・カンパニー・リミテッド | Methods, systems and filtration units for isolating particles from biological samples |
JP7350741B2 (en) * | 2017-12-01 | 2023-09-26 | グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー | Methods for cell enrichment and isolation |
WO2019109053A1 (en) | 2017-12-01 | 2019-06-06 | Juno Therapeutics, Inc. | Methods for dosing and for modulation of genetically engineered cells |
GB201720405D0 (en) | 2017-12-07 | 2018-01-24 | Biosafe Sa | A bioprocessing system |
CN112004824B (en) | 2017-12-08 | 2024-09-13 | 朱诺治疗学股份有限公司 | Process for producing engineered T cell compositions |
CN112203680A (en) | 2017-12-08 | 2021-01-08 | 朱诺治疗学股份有限公司 | Phenotypic markers for cell therapy and related methods |
SG11202005228YA (en) | 2017-12-08 | 2020-07-29 | Juno Therapeutics Inc | Serum-free media formulation for culturing cells and methods of use thereof |
US12006356B2 (en) | 2017-12-15 | 2024-06-11 | Juno Therapeutics, Inc. | Anti-CCT5 binding molecules and chimeric antigen receptors comprising the same |
CN111566221B (en) | 2017-12-20 | 2023-07-25 | 美天施生物科技有限两合公司 | Methods for NK cell transduction |
US11701387B2 (en) | 2018-01-04 | 2023-07-18 | Miltenyi Biotec B.V. & Co. KG | Chimeric antigen receptor specific for BDCA2 antigen |
JP2021511041A (en) | 2018-01-19 | 2021-05-06 | ミルテニイ バイオテック ベー.ファオ. ウント コー.カーゲー | Regulatory T cells expressing chimeric antigen receptor |
MA54118A (en) | 2018-01-31 | 2021-09-15 | Celgene Corp | MULTIPLE THERAPY USING ADOPTIVE CELL THERAPY AND A CHECKPOINT INHIBITOR |
JP2021511802A (en) | 2018-01-31 | 2021-05-13 | ジュノー セラピューティクス インコーポレイテッド | Methods and Reagents for Assessing the Presence or Absence of Replicate Virus |
MY197176A (en) | 2018-02-01 | 2023-05-30 | Nkmax Co Ltd | Method of producing natural killer cells and composition for treating cancer |
US11920119B2 (en) | 2018-02-09 | 2024-03-05 | Global Life Sciences Solutions Usa Llc | Systems and methods for bioprocessing |
US11932842B2 (en) | 2018-02-09 | 2024-03-19 | Global Life Sciences Solutions Usa Llc | Bioprocessing apparatus |
US12077743B2 (en) | 2018-02-09 | 2024-09-03 | Global Life Sciences Solutions Usa Llc | Apparatus for fluid line management in a bioprocessing system |
KR20200136409A (en) | 2018-02-28 | 2020-12-07 | 주노 쎄러퓨티크스 인코퍼레이티드 | Method for detecting particles present in cell composition |
WO2019170845A1 (en) | 2018-03-09 | 2019-09-12 | Ospedale San Raffaele S.R.L. | Il-1 antagonist and toxicity induced by cell therapy |
US20210015869A1 (en) | 2018-04-05 | 2021-01-21 | Juno Therapeutics, Inc. | T cells expressing a recombinant receptor, related polynucleotides and methods |
TWI840351B (en) | 2018-04-05 | 2024-05-01 | 美商奇諾治療有限公司 | T cell receptors and engineered cells expressing same |
CA3094468A1 (en) | 2018-04-05 | 2019-10-10 | Juno Therapeutics, Inc. | Methods of producing cells expressing a recombinant receptor and related compositions |
CN112136034A (en) | 2018-04-27 | 2020-12-25 | 贝克顿·迪金森公司 | Collection system for flow cytometry sorting samples and methods of use thereof |
WO2019209714A1 (en) | 2018-04-27 | 2019-10-31 | Becton, Dickinson And Company | Flow cytometers having enclosed droplet sorters with controlled aerosol content and methods of using the same |
CA3098497A1 (en) | 2018-05-03 | 2019-11-07 | Juno Therapeutics, Inc. | Combination therapy of a chimeric antigen receptor (car) t cell therapy and a kinase inhibitor |
US11413378B2 (en) | 2018-05-08 | 2022-08-16 | Biomagnetic Solutions Llc | Rigid chamber for cell separation from a flexible disposable bag |
IL278630B1 (en) | 2018-05-11 | 2024-06-01 | Lupagen Inc | Systems and methods for closed loop, real-time modifications of patient cells |
WO2019234033A1 (en) | 2018-06-06 | 2019-12-12 | Pierson Yann | Apparatus and process for the automated manufacturing of genetically engineered cells from biological fluids |
SG11202012299SA (en) | 2018-06-13 | 2021-01-28 | Akron Biotechnology Llc | Method to prepare therapeutically active aldesleukin highly stable in liquid pharmaceutical compositions |
KR20210069032A (en) * | 2018-07-27 | 2021-06-10 | 유니버셀스 테크놀로지스 에스.에이. | Biomolecule production systems and methods |
EP3833759A1 (en) | 2018-08-09 | 2021-06-16 | Juno Therapeutics, Inc. | Methods for assessing integrated nucleic acids |
AU2019318560A1 (en) | 2018-08-09 | 2021-02-25 | Juno Therapeutics, Inc. | Processes for generating engineered cells and compositions thereof |
US10988726B2 (en) | 2018-08-19 | 2021-04-27 | Cytera Cellworks Limited | Systems and methods for automated cell culturing |
WO2020043899A1 (en) | 2018-08-31 | 2020-03-05 | Invectys | Chimeric antigen receptors against multiple hla-g isoforms |
US20220050114A1 (en) | 2018-09-11 | 2022-02-17 | Juno Therapeutics, Inc. | Methods for mass spectrometry analysis of engineered cell compositions |
WO2020091866A1 (en) | 2018-10-30 | 2020-05-07 | Becton, Dickinson And Company | Particle sorting module with alignment window, systems and methods of use thereof |
MX2021005024A (en) | 2018-11-01 | 2021-07-21 | Juno Therapeutics Inc | Methods for treatment using chimeric antigen receptors specific for b-cell maturation antigen. |
TW202021981A (en) | 2018-11-01 | 2020-06-16 | 美商奇諾治療有限公司 | Chimeric antigen receptors specific for g protein-coupled receptor class c group 5 member d (gprc5d) |
MX2021005021A (en) | 2018-11-06 | 2021-08-11 | Juno Therapeutics Inc | Process for producing genetically engineered t cells. |
KR20210111247A (en) | 2018-11-08 | 2021-09-10 | 주노 쎄러퓨티크스 인코퍼레이티드 | Methods and Combinations for Treatment and Modulation of T Cells |
US20220008465A1 (en) | 2018-11-16 | 2022-01-13 | Juno Therapeutics, Inc. | Methods of dosing engineered t cells for the treatment of b cell malignancies |
FI3886894T3 (en) | 2018-11-30 | 2024-05-24 | Juno Therapeutics Inc | Methods for dosing and treatment of b cell malignancies in adoptive cell therapy |
PT3886875T (en) | 2018-11-30 | 2024-06-27 | Juno Therapeutics Inc | Methods for treatment using adoptive cell therapy |
WO2020124231A1 (en) | 2018-12-21 | 2020-06-25 | Octane Biotech Inc. | Carousel for modular biologic production units |
EP3898994A4 (en) | 2018-12-21 | 2022-12-14 | Lonza Walkersville, Inc. | Automated production of viral vectors |
WO2020132743A1 (en) | 2018-12-28 | 2020-07-02 | Octane Biotech Inc. | Cell culture and tissue engineering systems with controlled environmental zones |
US20220096651A1 (en) | 2019-01-29 | 2022-03-31 | Juno Therapeutics, Inc. | Antibodies and chimeric antigen receptors specific for receptor tyrosine kinase like orphan receptor 1 (ror1) |
SG11202108473XA (en) | 2019-02-08 | 2021-09-29 | Lonza Walkersville Inc | Cell concentration methods and devices for use in automated bioreactors |
DE102019106651A1 (en) | 2019-03-15 | 2020-09-17 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Treatment device and method for extracorporeal, immune tolerance-increasing blood treatment |
US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
WO2020223535A1 (en) | 2019-05-01 | 2020-11-05 | Juno Therapeutics, Inc. | Cells expressing a recombinant receptor from a modified tgfbr2 locus, related polynucleotides and methods |
JP2022531577A (en) | 2019-05-01 | 2022-07-07 | ジュノー セラピューティクス インコーポレイテッド | Cells expressing chimeric receptors from the modified CD247 locus, related polynucleotides, and methods. |
CN114072490A (en) | 2019-05-07 | 2022-02-18 | 伯乐实验室有限公司 | System and method for automated single cell processing |
US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
CA3142108A1 (en) | 2019-05-28 | 2020-12-03 | Miltenyi Biotec B.V. & Co. KG | Method for generation of genetically modified t cells |
MX2021015317A (en) | 2019-06-12 | 2022-03-11 | Juno Therapeutics Inc | Combination therapy of a cell-mediated cytotoxic therapy and an inhibitor of a prosurvival bcl2 family protein. |
CA3143241A1 (en) | 2019-06-14 | 2020-12-17 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing and analyses |
JP2022544851A (en) | 2019-08-22 | 2022-10-21 | マイケル・イアノッティ | High-throughput analysis and sorting, and sampling interfaces and assemblies for high-throughput analysis and sorting |
KR20220066892A (en) | 2019-08-22 | 2022-05-24 | 주노 쎄러퓨티크스 인코퍼레이티드 | Combination therapy of T cell therapy and Zest homologue 2 enhancer (EH2) inhibitor and related methods |
CN114600172A (en) | 2019-08-30 | 2022-06-07 | 朱诺治疗学股份有限公司 | Machine learning method for classifying cells |
US20220315894A1 (en) | 2019-09-11 | 2022-10-06 | Miltenyi Biotec B.V. & Co. KG | Method for Transduction of T Cells in the Presence of Malignant Cells |
ES2960527T3 (en) | 2019-09-13 | 2024-03-05 | Miltenyi Biotec Bv & Co Kg | Method for the generation of a cellular composition of dopaminergic progenitor cells of the ventral midbrain |
CN115087868A (en) | 2019-11-05 | 2022-09-20 | 朱诺治疗学股份有限公司 | Methods of determining attributes of therapeutic T cell compositions |
EP4054623A1 (en) | 2019-11-07 | 2022-09-14 | Juno Therapeutics, Inc. | Combination of a t cell therapy and (s)-3-[4-(4-morpholin-4 ylmethyl-benzyloxy)-l-oxo-l,3-dihydro-isoindol-2-yl]- piperidine-2,6-dione |
US11000780B1 (en) | 2019-12-06 | 2021-05-11 | AnuCell Biosystems Limited | Torus reactor for a combined cell isolator and bioreactor |
WO2021113780A1 (en) | 2019-12-06 | 2021-06-10 | Juno Therapeutics, Inc. | Anti-idiotypic antibodies to gprc5d-targeted binding domains and related compositions and methods |
WO2021113776A1 (en) | 2019-12-06 | 2021-06-10 | Juno Therapeutics, Inc. | Anti-idiotypic antibodies to bcma-targeted binding domains and related compositions and methods |
BR112022010310A2 (en) | 2019-12-06 | 2022-08-16 | Juno Therapeutics Inc | METHODS RELATED TO TOXICITY AND RESPONSE ASSOCIATED WITH CELL THERAPY FOR TREATMENT OF B-CELL MAGNITIES |
US20230071910A1 (en) | 2020-01-24 | 2023-03-09 | Juno Therapeutics, Inc. | Methods for dosing and treatment of follicular lymphoma and marginal zone lymphoma in adoptive cell therapy |
EP4097218A1 (en) | 2020-01-28 | 2022-12-07 | Juno Therapeutics, Inc. | Methods for t cell transduction |
US20210260600A1 (en) * | 2020-02-01 | 2021-08-26 | Sunil Mehta | Automated device and method to purify biomaterials from a mixture by using magnetic particles and disposable product-contact materials |
WO2021163389A1 (en) | 2020-02-12 | 2021-08-19 | Juno Therapeutics, Inc. | Bcma-directed chimeric antigen receptor t cell compositions and methods and uses thereof |
CN115768443A (en) | 2020-02-12 | 2023-03-07 | 朱诺治疗学股份有限公司 | CD19 directed chimeric antigen receptor T cell compositions and methods and uses thereof |
CA3175039A1 (en) | 2020-03-10 | 2021-09-16 | Cellares Corporation | Systems, devices, and methods for cell processing |
EP4127127A1 (en) * | 2020-03-27 | 2023-02-08 | Broadley-James Corporation | Single-use bioreactor assembly with integrated pump heads |
CN115916223A (en) | 2020-04-10 | 2023-04-04 | 朱诺治疗学股份有限公司 | Methods and uses related to cell therapy engineered with chimeric antigen receptors targeting B cell maturation antigens |
AU2021263765A1 (en) | 2020-04-28 | 2022-12-01 | Juno Therapeutics, Inc. | Combination of BCMA-directed T cell therapy and an immunomodulatory compound |
CN115803824A (en) | 2020-05-13 | 2023-03-14 | 朱诺治疗学股份有限公司 | Methods of identifying characteristics associated with clinical response and uses thereof |
US20230181641A1 (en) | 2020-05-13 | 2023-06-15 | Juno Therapeutics, Inc. | Process for producing donor-batched cells expressing a recombinant receptor |
WO2021262147A1 (en) * | 2020-06-23 | 2021-12-30 | Qualigen Inc. | Devices and methods for on-line whole blood treatment |
JP2023531531A (en) | 2020-06-26 | 2023-07-24 | ジュノ セラピューティクス ゲーエムベーハー | Engineered T Cells Conditionally Expressing Recombinant Receptors, Related Polynucleotides, and Methods |
CA3186849A1 (en) | 2020-07-23 | 2022-01-27 | Stefan Robbert BRAAM | Closed manufacturing processes for large scale manufacturing of pluripotent stem cell derived cells |
CN111992339B (en) * | 2020-07-28 | 2022-02-11 | 刘肖琳 | Centrifugal separation device, and method for separating and culturing mixed liquid |
WO2022029660A1 (en) | 2020-08-05 | 2022-02-10 | Juno Therapeutics, Inc. | Anti-idiotypic antibodies to ror1-targeted binding domains and related compositions and methods |
KR102581230B1 (en) | 2020-10-16 | 2023-09-21 | 의료법인 성광의료재단 | Natural killer cells with regulated gene expression of anti-cancer effect and uses thereof |
US20220127558A1 (en) * | 2020-10-23 | 2022-04-28 | Applied Materials, Inc. | Automated closed system for cell therapy manufacturing |
JP2023549780A (en) | 2020-11-04 | 2023-11-29 | ジュノー セラピューティクス インコーポレイテッド | Cells expressing chimeric receptors from engineered invariant CD3 immunoglobulin superfamily chain loci and related polynucleotides and methods |
WO2022096425A2 (en) | 2020-11-04 | 2022-05-12 | Miltenyi Biotec B.V. & Co. KG | Chimeric antigen receptor comprising an antigen binding domain specific for msln having a specificity for tumor cells |
WO2022133030A1 (en) | 2020-12-16 | 2022-06-23 | Juno Therapeutics, Inc. | Combination therapy of a cell therapy and a bcl2 inhibitor |
KR20230137402A (en) | 2021-02-20 | 2023-10-04 | 카이트 파마 인코포레이티드 | Genetic markers for immunotherapy selection |
CN117693508A (en) | 2021-03-03 | 2024-03-12 | 朱诺治疗学股份有限公司 | Combination of T cell therapy and DGK inhibitors |
EP4310478A1 (en) * | 2021-03-16 | 2024-01-24 | Sony Group Corporation | Closed-system automatic sample sorting system |
AU2022241654A1 (en) | 2021-03-22 | 2023-09-28 | Juno Therapeutics, Inc. | Methods of determining potency of a therapeutic cell composition |
JP2024514245A (en) | 2021-03-29 | 2024-03-29 | ジュノー セラピューティクス インコーポレイテッド | Methods for dosing and treatment using a combination of checkpoint inhibitor therapy and CAR T cell therapy |
AU2022252220A1 (en) | 2021-03-29 | 2023-10-12 | Juno Therapeutics, Inc. | Combination of a car t cell therapy and an immunomodulatory compound for treatment of lymphoma |
US20240207310A1 (en) | 2021-04-16 | 2024-06-27 | Celgene Corporation | Combination therapies with bcma-directed t cell therapy |
IL308227A (en) | 2021-05-14 | 2024-01-01 | Kite Pharma Inc | Chimeric antigen receptor t cell therapy |
EP4413031A1 (en) | 2021-10-06 | 2024-08-14 | Miltenyi Biotec B.V. & Co. KG | Method for targeted gene insertion into immune cells |
WO2023062113A1 (en) | 2021-10-15 | 2023-04-20 | Miltenyi Biotec B.V. & Co. KG | Method for the generation of genetically modified nk cells |
WO2023081735A1 (en) | 2021-11-03 | 2023-05-11 | Celgene Corporation | Chimeric antigen receptors specific for b-cell maturation antigen for use in treating myeloma |
WO2023081900A1 (en) | 2021-11-08 | 2023-05-11 | Juno Therapeutics, Inc. | Engineered t cells expressing a recombinant t cell receptor (tcr) and related systems and methods |
WO2023147515A1 (en) | 2022-01-28 | 2023-08-03 | Juno Therapeutics, Inc. | Methods of manufacturing cellular compositions |
WO2023150285A1 (en) | 2022-02-04 | 2023-08-10 | Elixirgen Therapeutics, Inc. | Cell therapy protocol systems and methods |
US20230296610A1 (en) | 2022-02-15 | 2023-09-21 | Kite Pharma, Inc. | Predicting adverse events from immunotherapy |
AU2023223404A1 (en) | 2022-02-22 | 2024-08-29 | Juno Therapeutics, Inc. | Proteinase 3 (pr3) chimeric autoantibody receptor t cells and related methods and uses |
WO2023209175A1 (en) | 2022-04-28 | 2023-11-02 | Bio-Recell Ltd. | Method for separating target cells |
WO2023220655A1 (en) | 2022-05-11 | 2023-11-16 | Celgene Corporation | Methods to overcome drug resistance by re-sensitizing cancer cells to treatment with a prior therapy via treatment with a t cell therapy |
WO2023225048A1 (en) * | 2022-05-17 | 2023-11-23 | Synthego Corporation | Systems and methods for engageable platforms and modules for cell processing |
WO2023230581A1 (en) | 2022-05-25 | 2023-11-30 | Celgene Corporation | Methods of manufacturing t cell therapies |
WO2023230548A1 (en) | 2022-05-25 | 2023-11-30 | Celgene Corporation | Method for predicting response to a t cell therapy |
WO2023230276A1 (en) | 2022-05-27 | 2023-11-30 | Kite Pharma, Inc. | Compositions and methods for preparing engineered lymphocytes for cell therapy |
WO2023250400A1 (en) | 2022-06-22 | 2023-12-28 | Juno Therapeutics, Inc. | Treatment methods for second line therapy of cd19-targeted car t cells |
DE102022115737B3 (en) | 2022-06-24 | 2023-07-06 | Imstec Gmbh | Device and method for automated cell cultivation |
WO2024006960A1 (en) | 2022-06-29 | 2024-01-04 | Juno Therapeutics, Inc. | Lipid nanoparticles for delivery of nucleic acids |
US20240041929A1 (en) | 2022-08-05 | 2024-02-08 | Juno Therapeutics, Inc. | Chimeric antigen receptors specific for gprc5d and bcma |
WO2024044779A2 (en) | 2022-08-26 | 2024-02-29 | Juno Therapeutics, Inc. | Antibodies and chimeric antigen receptors specific for delta-like ligand 3 (dll3) |
WO2024054944A1 (en) | 2022-09-08 | 2024-03-14 | Juno Therapeutics, Inc. | Combination of a t cell therapy and continuous or intermittent dgk inhibitor dosing |
WO2024078995A1 (en) | 2022-10-15 | 2024-04-18 | Miltenyi Biotec B.V. & Co. KG | Transduction of gammadelta t cells with pseudotyped retroviral vectors |
WO2024092227A1 (en) | 2022-10-28 | 2024-05-02 | Kite Pharma, Inc. | Factors for optimizing immunotherapy |
WO2024092145A1 (en) | 2022-10-28 | 2024-05-02 | Kite Pharma, Inc. | Expedited administration of engineered lymphocytes |
WO2024097905A1 (en) | 2022-11-02 | 2024-05-10 | Celgene Corporation | Methods of treatment with t cell therapy and immunomodulatory agent maintenance therapy |
WO2024097992A2 (en) | 2022-11-04 | 2024-05-10 | Umoja Biopharma, Inc. | Particles displaying adhesion-molecule fusions |
WO2024098028A2 (en) | 2022-11-04 | 2024-05-10 | Umoja Biopharma, Inc. | Lentiviral particles displaying fusion molecules and uses thereof |
WO2024129778A2 (en) | 2022-12-13 | 2024-06-20 | Juno Therapeutics, Inc. | Chimeric antigen receptors specific for baff-r and cd19 and methods and uses thereof |
US20240285762A1 (en) | 2023-02-28 | 2024-08-29 | Juno Therapeutics, Inc. | Cell therapy for treating systemic autoimmune diseases |
US11939565B1 (en) * | 2023-06-28 | 2024-03-26 | Trisk Bio Ltd. | Methods and systems for performing biotechnological processes |
US12013066B1 (en) | 2023-11-03 | 2024-06-18 | Trisk Bio Ltd. | Methods and systems for reversibly consummating fluidic connections |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4632908A (en) * | 1984-05-03 | 1986-12-30 | Abbott Laboratories | Heating system for rotating members |
US5260598A (en) * | 1991-10-04 | 1993-11-09 | Fresenius Ag | Device for separation of media into their components having means for detection and adjustment of the phase boundary |
EP0654669A2 (en) * | 1993-11-19 | 1995-05-24 | Bristol-Myers Squibb Company | Liquid separation apparatus and method |
US6709377B1 (en) * | 1999-04-09 | 2004-03-23 | Haemonetics Corporation | System and method for quick disconnect centrifuge unit |
Family Cites Families (45)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3586413A (en) * | 1969-03-25 | 1971-06-22 | Dale A Adams | Apparatus for providing energy communication between a moving and a stationary terminal |
US3955755A (en) * | 1975-04-25 | 1976-05-11 | The United States Of America As Represented By The United States Energy Research And Development Administration | Closed continuous-flow centrifuge rotor |
US4230685A (en) | 1979-02-28 | 1980-10-28 | Northwestern University | Method of magnetic separation of cells and the like, and microspheres for use therein |
US4321330A (en) * | 1980-04-04 | 1982-03-23 | Baker Fraser L | Tissue culture device |
US4452773A (en) | 1982-04-05 | 1984-06-05 | Canadian Patents And Development Limited | Magnetic iron-dextran microspheres |
JPS614554A (en) * | 1984-06-15 | 1986-01-10 | Hitachi Koki Co Ltd | Continuous centrifugal rotor |
US5266476A (en) * | 1985-06-18 | 1993-11-30 | Yeda Research & Development Co., Ltd. | Fibrous matrix for in vitro cell cultivation |
US4795698A (en) | 1985-10-04 | 1989-01-03 | Immunicon Corporation | Magnetic-polymer particles |
CA1335181C (en) * | 1988-10-11 | 1995-04-11 | R. Alan Hardwick | System for selective cell separation from cell concentrate |
EP0452342B1 (en) | 1988-12-28 | 1994-11-30 | MILTENYI, Stefan | Methods and materials for high gradient magnetic separation of biological materials |
US5316667A (en) * | 1989-05-26 | 1994-05-31 | Baxter International Inc. | Time based interface detection systems for blood processing apparatus |
US6143508A (en) * | 1989-06-29 | 2000-11-07 | Rhone-Poulenc Rorer Pharmaceuticals Inc. | Device and process for cell capture and recovery |
US5242606A (en) * | 1990-06-04 | 1993-09-07 | Abaxis, Incorporated | Sample metering port for analytical rotor having overflow chamber |
US5200084A (en) | 1990-09-26 | 1993-04-06 | Immunicon Corporation | Apparatus and methods for magnetic separation |
AU5283393A (en) * | 1992-10-14 | 1994-05-09 | Andrew George Bosanquet | Method and apparatus for conducting tests, particularly comparative tests |
WO1994008689A1 (en) * | 1992-10-22 | 1994-04-28 | Baxter International Inc. | Blood processing systems with improved data transfer between stationary and rotating elements |
US5705059A (en) | 1995-02-27 | 1998-01-06 | Miltenyi; Stefan | Magnetic separation apparatus |
DE69834397T2 (en) * | 1997-05-20 | 2007-06-06 | Zymequest, Inc., Beverly | Device for expelling fluids |
US5935051A (en) * | 1997-08-29 | 1999-08-10 | Beckman Instruments, Inc. | Blood separation device |
US5904840A (en) * | 1998-04-06 | 1999-05-18 | Dibella; Alberto | Apparatus for accurate centrifugal separation of miscible and immiscible media |
US6080581A (en) * | 1998-07-02 | 2000-06-27 | Charles Daniel Anderson | Culture vessel for growing or culturing cells, cellular aggregates, tissues and organoids and methods for using same |
KR20010099700A (en) * | 1998-10-15 | 2001-11-09 | 추후제출 | Method for production of stroma-free hemoglobin |
EP1043072A1 (en) * | 1999-04-09 | 2000-10-11 | Jean-Denis Rochat | Centrifuging device and use of the device |
US6537593B2 (en) * | 2000-10-30 | 2003-03-25 | Council Of Scientific And Industrial Research | Plant phenol as new hepatoprotective agents |
US20020144939A1 (en) | 2001-04-09 | 2002-10-10 | Dolecek Victor D. | Miniaturized blood centrifuge having side mounted motor with belt drive |
US20020173034A1 (en) * | 2001-05-21 | 2002-11-21 | Emilio Barbera-Guillem | Centrifuge apparatus and methods for separating components from a cell culture device |
DE10127068A1 (en) | 2001-05-23 | 2002-11-28 | Bio Medical Apherese Systeme G | Apparatus for the separation of leukocytes, from blood, has a number of stations to separate marked components with magnetic particles without any adverse effects on the blood |
US6673595B2 (en) * | 2001-08-27 | 2004-01-06 | Biocrystal, Ltd | Automated cell management system for growth and manipulation of cultured cells |
ATE479490T1 (en) * | 2001-10-11 | 2010-09-15 | Aviva Biosciences Corp | METHOD FOR SEPARATING RARE CELLS FROM FLUID SAMPLES |
JP2003250882A (en) * | 2001-12-28 | 2003-09-09 | Katsutoshi Naruse | Artificial organ system using new perfusion method |
US7118522B2 (en) * | 2003-04-15 | 2006-10-10 | Beckman Coulter, Inc. | Centrifuge adapter |
BRPI0411798A (en) * | 2003-06-25 | 2006-08-08 | Macropore Biosurgery Inc | systems and methods for separating and concentrating regenerative cells from tissue |
CA2799683C (en) * | 2003-07-02 | 2014-03-25 | Terumo Bct, Inc. | Monitoring and control system for blood processing |
US7456019B2 (en) * | 2004-06-30 | 2008-11-25 | Regenetech, Inc. | Three-dimensional cell to tissue development process |
AU2005299765A1 (en) * | 2004-10-22 | 2006-05-04 | Cryofacets, Inc. | System, chamber, and method for fractionation and elutriation of fluids containing particulate components |
JP4368810B2 (en) * | 2005-01-28 | 2009-11-18 | テルモ株式会社 | Filter device and blood component collection device |
WO2007024518A2 (en) * | 2005-08-22 | 2007-03-01 | Gambro Inc. | Apparatus and method for separating a composite liquid into at least two components |
JP4665673B2 (en) * | 2005-09-02 | 2011-04-06 | パナソニック株式会社 | Optical analyzer |
US20070102374A1 (en) * | 2005-11-04 | 2007-05-10 | Gambro, Inc. | Blood processing apparatus with controlled cell capture chamber and method background of the invention |
JP5456689B2 (en) | 2007-12-07 | 2014-04-02 | ミルテンイ バイオテック ゲーエムベーハー | A centrifuge that separates a sample into at least two components |
KR101622231B1 (en) | 2014-04-18 | 2016-05-18 | 엘지전자 주식회사 | Refrigerator |
JP6734283B2 (en) | 2015-01-21 | 2020-08-05 | フレッド ハッチンソン キャンサー リサーチ センター | Point of care and/or portable platform for gene therapy |
JP2020528284A (en) | 2017-09-01 | 2020-09-24 | ロンザ ウォーカーズヴィル,インコーポレーテッド | Automation of end-to-end cell therapy |
CN112752847A (en) | 2018-09-28 | 2021-05-04 | 奥克泰生物科技股份有限公司 | Magnetic separation |
WO2020132743A1 (en) | 2018-12-28 | 2020-07-02 | Octane Biotech Inc. | Cell culture and tissue engineering systems with controlled environmental zones |
-
2008
- 2008-12-08 JP JP2010536549A patent/JP5456689B2/en active Active
- 2008-12-08 EP EP17180590.6A patent/EP3338895B1/en active Active
- 2008-12-08 US US12/745,689 patent/US8747290B2/en not_active Expired - Fee Related
- 2008-12-08 EP EP11193362.8A patent/EP2433713B1/en active Active
- 2008-12-08 EP EP12178904.4A patent/EP2520643B8/en active Active
- 2008-12-08 AT AT08858277T patent/ATE528077T1/en not_active IP Right Cessation
- 2008-12-08 WO PCT/IB2008/003845 patent/WO2009072006A2/en active Application Filing
- 2008-12-08 US US12/745,688 patent/US8727132B2/en active Active
- 2008-12-08 NO NO08857318A patent/NO2227271T3/no unknown
- 2008-12-08 ES ES08857318.3T patent/ES2660180T3/en active Active
- 2008-12-08 DK DK08857318.3T patent/DK2227271T3/en active
- 2008-12-08 EP EP17202816.9A patent/EP3338896A1/en active Pending
- 2008-12-08 JP JP2010536548A patent/JP5670197B2/en not_active Expired - Fee Related
- 2008-12-08 DK DK12178904.4T patent/DK2520643T3/en active
- 2008-12-08 DK DK11193362.8T patent/DK2433713T3/en active
- 2008-12-08 HU HUE11193362A patent/HUE034888T2/en unknown
- 2008-12-08 ES ES11193362.8T patent/ES2640216T3/en active Active
- 2008-12-08 EP EP08858277A patent/EP2227334B1/en active Active
- 2008-12-08 ES ES12178904T patent/ES2764077T3/en active Active
- 2008-12-08 ES ES08858277T patent/ES2374863T3/en active Active
- 2008-12-08 EP EP08857318.3A patent/EP2227271B1/en active Active
- 2008-12-08 ES ES17180590T patent/ES2928557T3/en active Active
- 2008-12-08 WO PCT/IB2008/003823 patent/WO2009072003A2/en active Application Filing
-
2013
- 2013-07-25 JP JP2013154975A patent/JP2013240797A/en not_active Withdrawn
-
2014
- 2014-01-07 JP JP2014000746A patent/JP5968345B2/en active Active
- 2014-02-14 US US14/181,023 patent/US9625463B2/en active Active
- 2014-03-07 US US14/201,744 patent/US9714945B2/en active Active
-
2016
- 2016-01-21 US US15/003,069 patent/US10705090B2/en active Active
- 2016-05-12 JP JP2016095870A patent/JP2016153124A/en not_active Withdrawn
- 2016-12-30 US US15/395,215 patent/US10119970B2/en active Active
-
2017
- 2017-09-15 US US15/706,517 patent/US10620212B2/en active Active
-
2019
- 2019-11-05 US US16/675,072 patent/US10705091B2/en active Active
-
2020
- 2020-06-01 US US16/889,471 patent/US11499976B2/en active Active
-
2022
- 2022-04-20 US US17/725,388 patent/US11499977B2/en active Active
- 2022-04-20 US US17/725,509 patent/US11531031B2/en active Active
- 2022-12-16 US US18/083,279 patent/US11933787B2/en active Active
-
2024
- 2024-03-05 US US18/596,432 patent/US20240219392A1/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4632908A (en) * | 1984-05-03 | 1986-12-30 | Abbott Laboratories | Heating system for rotating members |
US5260598A (en) * | 1991-10-04 | 1993-11-09 | Fresenius Ag | Device for separation of media into their components having means for detection and adjustment of the phase boundary |
EP0654669A2 (en) * | 1993-11-19 | 1995-05-24 | Bristol-Myers Squibb Company | Liquid separation apparatus and method |
US6709377B1 (en) * | 1999-04-09 | 2004-03-23 | Haemonetics Corporation | System and method for quick disconnect centrifuge unit |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10155083B2 (en) | 2010-11-23 | 2018-12-18 | Haemonetics Corporation | Apheresis bowl with improved vibration characteristics |
US9682185B2 (en) | 2010-11-23 | 2017-06-20 | Haemonetics Corporation | Apheresis bowl with improved vibration characteristics |
EP2643091B1 (en) * | 2010-11-23 | 2019-01-09 | Haemonetics Corporation | Apheresis bowl with improved vibration characteristics |
US10006840B2 (en) | 2011-11-25 | 2018-06-26 | Miltenyi Biotec Gmbh | Technology for purifying NK cells and other cell types by concurrent gravity sedimentation and magnetic separation |
US12066365B2 (en) | 2012-02-23 | 2024-08-20 | Juno Therapeutics Gmbh | Chromatographic isolation of cells and other complex biological materials |
EP2664384A1 (en) | 2012-05-15 | 2013-11-20 | Miltenyi Biotec GmbH | Centrifugation chamber with deflector shields |
US20130310241A1 (en) * | 2012-05-15 | 2013-11-21 | Miltenyi Biotec Gmbh | Centrifugation chamber with deflectors |
EP2664383A1 (en) | 2012-05-15 | 2013-11-20 | Miltenyi Biotec GmbH | Centrifugation chamber with deflector shields |
US9586213B2 (en) | 2012-05-15 | 2017-03-07 | Miltenyi Biotec Gmbh | Centrifugation chamber with deflectors |
EP2821144A1 (en) | 2013-07-02 | 2015-01-07 | Miltenyi Biotec GmbH | Centrifugation chamber with gas-permeable membrane for cell cultivation |
US10053663B2 (en) | 2013-07-02 | 2018-08-21 | Miltenyi Biotec, Gmbh | Centrifugation chamber with gas permeable membrane layers for cell cultivation |
EP2821145A1 (en) | 2013-07-02 | 2015-01-07 | Miltenyi Biotec GmbH | Centrifugation chamber with gas-permeable membranes layers for cell cultivation |
CN113046321A (en) * | 2014-04-23 | 2021-06-29 | 朱诺治疗学股份有限公司 | Methods of isolating, culturing and genetically engineering immune cell populations for adoptive therapy |
CN109843317A (en) * | 2016-08-29 | 2019-06-04 | 哈肯萨克大学医学中心 | The composition and method for by the stemness rich in blood platelet fraction of the blood containing platelets-like cell in people being used to that adult cell to be made to reprogram |
EP3342855A1 (en) | 2016-12-27 | 2018-07-04 | Miltenyi Biotec GmbH | Cell composition depleted from tcrab and cd45ra positive cells |
CN108680430A (en) * | 2018-03-15 | 2018-10-19 | 水利部交通运输部国家能源局南京水利科学研究院 | A kind of tail residues centrifugal model seepage flow with consolidation control system and test method |
CN108680430B (en) * | 2018-03-15 | 2020-11-24 | 水利部交通运输部国家能源局南京水利科学研究院 | Tailing centrifugal model consolidation seepage control system and testing method |
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US9714945B2 (en) | Centrifuge for separating a sample into at least two components | |
CA2382371C (en) | Medical system, method and apparatus employing mems | |
JP4002720B2 (en) | Single cell long-term culture microscope | |
EP0654669B1 (en) | Liquid separation apparatus and method | |
CN103620373B (en) | Using the method for analyte of interest in filter and microstructured surface detection sample | |
US9101926B2 (en) | Method for separating a sample into density specific fractions | |
CN111514950A (en) | System and method for sample concentration and detection using separation fluid | |
US11084035B2 (en) | Apparatus for cell preparation | |
JP2005279507A (en) | Method of extracting microorganism by density gradient centrifugal separation | |
CN111683751B (en) | Sample rack | |
CN112126588A (en) | Separation capture system for specific cells in liquid sample | |
GB2624632A (en) | Sampling module for a bioreactor container |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 2010536549 Country of ref document: JP |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2008858277 Country of ref document: EP |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 08858277 Country of ref document: EP Kind code of ref document: A2 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 12745689 Country of ref document: US |